AT5G27210

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004430
Physical Location & Seq
Reverse (-)
9572066 .. 9574135
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27210.1

Sequence Viewer

Length: 894 bp
ATGCGAGTTCTCGACGAAATTGCAGAGTCACCATTTCTGATTTCACCGCTAAACCCTAATTCGACAGCCAATGGATATAGATGGGTCGATAAGTGCCATGGATTCTTACATAACACGGTTCTCGTTGCGGCTTCTCTCTTCTTCGTCGCTTACTTAGCTTACGAAGCGAAGAAGAGTCTGTCTAAACTCTCGAATCGGAGATCTTATATCATGATTGCTTATTATGGTTGTCTTTGGCTTGTTAGCTTGCTCAATCTTGCTTGGTGCTGTCTCCAGGGTTGGGAATGTACTCCTGGGAAAGAAGTTGTTTGGAATCTGTTAACTTTGTTCACAACATCTGGAATGTTGTTTCTGGAAGTAAGCTTGGTGGCTTTTCTCTTCCAAGGAAACTATGCAAGCGGTGCAGAAGCATTAACTAGAACGTTCCTAATCTCGGGTTTTATTGTTGCTCTTGATTTGCTTCTCAAGGCAATTTTTCTCTTTGGATTTGGAGTGCCATTGTTTATTGACAACAATGAAAATGGAAAAACGTTCAAATGGGGCTTATGGATCATTCATAAGCTTCTACTCACTGGCGTTTATGGGATGGTGTTCTTGATGTACAACTCTAGGTGGAGAGAAAAATTGCCTGCAAGGCCTGCATTCTACAACTACATAATCATCATGTTCGCCTTATACAGCCTTTACCTAGTTGCATCTGCGTTTACCGCAAACAACGCGCATTTTGGTTTCTGGTTATATGGGATCATGAGTGTTTGCTACCACGCATTATACCTTCCTCTTCTTTACATAACGTTCCTCGCAGACTTTTTCCAGGAAGAAGATTTGAACTTGGAGAATGTATACTACTCAGAGATGAAGGATGCTGGATTTTTCGACTCTGATTGGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

297

Amino Acids

34.14

Weight (kDa)

5.75

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_40 PF10160 31 - 284 3.6e-117 Predicted membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 843
AccII CGCG 1 cut(s) 719
AciI CCGC 4 cut(s) 47, 128, 399, 708
AclI AACGTT 3 cut(s) 422, 530, 794
AclWI GGATC 2 cut(s) 557, 752
AfaI GTAC 2 cut(s) 289, 602
AfiI CCNNNNNNNGG 2 cut(s) 280, 433
AgsI TTSAA 2 cut(s) 535, 829
AjnI CCWGG 3 cut(s) 273, 292, 813
AluBI AGCT 4 cut(s) 158, 246, 363, 562
AluI AGCT 4 cut(s) 158, 246, 363, 562
Alw26I GTCTC 1 cut(s) 275
AlwI GGATC 2 cut(s) 557, 752
Ama87I CYCGRG 1 cut(s) 433
AoxI GGCC 1 cut(s) 635
AspLEI GCGC 1 cut(s) 721
AsuHPI GGTGA 2 cut(s) 21, 36
AvaI CYCGRG 1 cut(s) 433
BccI CCATC 2 cut(s) 75, 580
BcgI CGANNNNNNTGC 1 cut(s) 36
BciT130I CCWGG 3 cut(s) 275, 294, 815
BcoDI GTCTC 1 cut(s) 275
BfaI CTAG 3 cut(s) 417, 609, 689
BglI GCCNNNNNGGC 1 cut(s) 634
BglII AGATCT 1 cut(s) 200
BisI GCNGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 130
Bme1390I CCNGG 3 cut(s) 275, 294, 815
BmeT110I CYCGRG 1 cut(s) 433
BmrFI CCNGG 3 cut(s) 275, 294, 815
BmsI GCATC 2 cut(s) 704, 853
BpmI CTGGAG 1 cut(s) 257
BpuEI CTTGAG 1 cut(s) 449
BsaJI CCNNGG 4 cut(s) 97, 274, 293, 382
Bsc4I CCNNNNNNNGG 2 cut(s) 280, 433
Bse1I ACTGG 1 cut(s) 577
BseBI CCWGG 3 cut(s) 275, 294, 815
BseDI CCNNGG 4 cut(s) 97, 274, 293, 382
BseGI GGATG 2 cut(s) 591, 868
BseLI CCNNNNNNNGG 2 cut(s) 280, 433
BseMII CTCAG 1 cut(s) 864
BseNI ACTGG 1 cut(s) 577
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 1 cut(s) 719
BshFI GGCC 1 cut(s) 637
BsiHKCI CYCGRG 1 cut(s) 433
BslI CCNNNNNNNGG 2 cut(s) 280, 433
BsmAI GTCTC 1 cut(s) 275
BsmI GAATGC 1 cut(s) 641
BsnI GGCC 1 cut(s) 637
BsoBI CYCGRG 1 cut(s) 433
Bsp1407I TGTACA 1 cut(s) 600
Bsp143I GATC 3 cut(s) 200, 549, 744
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 4 cut(s) 47, 128, 399, 708
BspANI GGCC 1 cut(s) 637
BspCNI CTCAG 1 cut(s) 863
BspFNI CGCG 1 cut(s) 719
BspHI TCATGA 2 cut(s) 210, 747
BspPI GGATC 2 cut(s) 557, 752
BsrGI TGTACA 1 cut(s) 600
BsrI ACTGG 1 cut(s) 577
BssECI CCNNGG 4 cut(s) 97, 274, 293, 382
BssMI GATC 3 cut(s) 200, 549, 744
BssNAI GTATAC 1 cut(s) 844
BssT1I CCWWGG 2 cut(s) 97, 382
Bst1107I GTATAC 1 cut(s) 844
Bst2UI CCWGG 3 cut(s) 275, 294, 815
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 4 cut(s) 143, 167, 383, 786
BstAPI GCANNNNNTGC 2 cut(s) 401, 638
BstAUI TGTACA 1 cut(s) 600
BstC8I GCNNGC 4 cut(s) 248, 397, 630, 639
BstDEI CTNAG 2 cut(s) 154, 850
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 2 cut(s) 591, 868
BstFNI CGCG 1 cut(s) 719
BstHHI GCGC 1 cut(s) 721
BstKTI GATC 3 cut(s) 203, 552, 747
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 3 cut(s) 200, 549, 744
BstMWI GCNNNNNNNGC 7 cut(s) 155, 164, 401, 634, 638, 707, 716
BstNI CCWGG 3 cut(s) 275, 294, 815
BstSCI CCNGG 3 cut(s) 273, 292, 813
BstUI CGCG 1 cut(s) 719
BstX2I RGATCY 1 cut(s) 200
BstYI RGATCY 1 cut(s) 200
BstZ17I GTATAC 1 cut(s) 844
BsuRI GGCC 1 cut(s) 637
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 2 cut(s) 591, 868
BtsIMutI CAGTG 1 cut(s) 570
Cac8I GCNNGC 4 cut(s) 248, 397, 630, 639
CciI TCATGA 2 cut(s) 210, 747
CfoI GCGC 1 cut(s) 721
Csp6I GTAC 2 cut(s) 288, 601
CviAII CATG 4 cut(s) 98, 211, 664, 748
CviQI GTAC 2 cut(s) 288, 601
DdeI CTNAG 2 cut(s) 154, 850
DpnI GATC 3 cut(s) 202, 551, 746
DpnII GATC 3 cut(s) 200, 549, 744
Eam1104I CTCTTC 4 cut(s) 143, 167, 383, 786
EarI CTCTTC 4 cut(s) 143, 167, 383, 786
Eco130I CCWWGG 2 cut(s) 97, 382
Eco147I AGGCCT 1 cut(s) 637
Eco88I CYCGRG 1 cut(s) 433
EcoRII CCWGG 3 cut(s) 273, 292, 813
EcoT14I CCWWGG 2 cut(s) 97, 382
ErhI CCWWGG 2 cut(s) 97, 382
FaeI CATG 4 cut(s) 101, 214, 667, 751
FatI CATG 4 cut(s) 97, 210, 663, 747
FblI GTMKAC 1 cut(s) 843
Fnu4HI GCNGC 1 cut(s) 129
FokI GGATG 2 cut(s) 598, 875
Fsp4HI GCNGC 1 cut(s) 129
FspBI CTAG 3 cut(s) 417, 609, 689
GlaI GCGC 1 cut(s) 720
GluI GCNGC 1 cut(s) 129
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 1 cut(s) 637
HhaI GCGC 1 cut(s) 721
Hin1II CATG 4 cut(s) 101, 214, 667, 751
Hin6I GCGC 1 cut(s) 719
HinP1I GCGC 1 cut(s) 719
HincII GTYRAC 1 cut(s) 321
HindII GTYRAC 1 cut(s) 321
HindIII AAGCTT 2 cut(s) 361, 560
HinfI GANTC 6 cut(s) 26, 102, 175, 193, 313, 878
HpaI GTTAAC 1 cut(s) 321
HphI GGTGA 2 cut(s) 21, 36
Hpy166II GTNNAC 4 cut(s) 321, 330, 705, 844
Hpy188I TCNGA 4 cut(s) 39, 198, 853, 883
Hpy188III TCNNGA 8 cut(s) 11, 190, 211, 339, 353, 452, 595, 748
Hpy8I GTNNAC 4 cut(s) 321, 330, 705, 844
Hpy99I CGWCG 2 cut(s) 17, 149
HpyAV CCTTC 2 cut(s) 785, 853
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4IV ACGT 3 cut(s) 422, 530, 794
HpyCH4V TGCA 6 cut(s) 23, 395, 404, 632, 641, 695
HpyF10VI GCNNNNNNNGC 7 cut(s) 155, 164, 401, 634, 638, 707, 716
HpyF3I CTNAG 2 cut(s) 154, 850
HpySE526I ACGT 3 cut(s) 422, 530, 794
Hsp92II CATG 4 cut(s) 101, 214, 667, 751
HspAI GCGC 1 cut(s) 719
KspAI GTTAAC 1 cut(s) 321
Kzo9I GATC 3 cut(s) 200, 549, 744
LweI GCATC 2 cut(s) 704, 853
MaeI CTAG 3 cut(s) 417, 609, 689
MaeII ACGT 3 cut(s) 422, 530, 794
MaeIII GTNAC 1 cut(s) 27
MalI GATC 3 cut(s) 202, 551, 746
MboI GATC 3 cut(s) 200, 549, 744
MboII GAAGA 8 cut(s) 130, 133, 181, 184, 370, 773, 830, 833
MflI RGATCY 1 cut(s) 200
MluCI AATT 4 cut(s) 18, 58, 471, 623
MlyI GAGTC 3 cut(s) 35, 184, 872
MnlI CCTC 2 cut(s) 789, 809
MseI TTAA 2 cut(s) 320, 413
MspR9I CCNGG 3 cut(s) 275, 294, 815
Mva1269I GAATGC 1 cut(s) 641
MvaI CCWGG 3 cut(s) 275, 294, 815
MvnI CGCG 1 cut(s) 719
MwoI GCNNNNNNNGC 7 cut(s) 155, 164, 401, 634, 638, 707, 716
NcoI CCATGG 1 cut(s) 97
NdeII GATC 3 cut(s) 200, 549, 744
NlaIII CATG 4 cut(s) 101, 214, 667, 751
NmuCI GTSAC 1 cut(s) 27
PagI TCATGA 2 cut(s) 210, 747
PceI AGGCCT 1 cut(s) 637
PctI GAATGC 1 cut(s) 641
PfeI GAWTC 3 cut(s) 102, 193, 313
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 1 cut(s) 130
PleI GAGTC 3 cut(s) 34, 183, 872
PpsI GAGTC 3 cut(s) 34, 183, 872
Psp1406I AACGTT 3 cut(s) 422, 530, 794
Psp6I CCWGG 3 cut(s) 273, 292, 813
PspGI CCWGG 3 cut(s) 273, 292, 813
PsuI RGATCY 1 cut(s) 200
RsaI GTAC 2 cut(s) 289, 602
RsaNI GTAC 2 cut(s) 288, 601
SaqAI TTAA 2 cut(s) 320, 413
SatI GCNGC 1 cut(s) 129
Sau3AI GATC 3 cut(s) 200, 549, 744
SchI GAGTC 3 cut(s) 35, 184, 872
ScrFI CCNGG 3 cut(s) 275, 294, 815
SfaNI GCATC 2 cut(s) 704, 853
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 4 cut(s) 18, 58, 471, 623
SseBI AGGCCT 1 cut(s) 637
SsiI CCGC 4 cut(s) 47, 128, 399, 708
SspMI CTAG 3 cut(s) 417, 609, 689
StuI AGGCCT 1 cut(s) 637
StyD4I CCNGG 3 cut(s) 273, 292, 813
StyI CCWWGG 2 cut(s) 97, 382
TaaI ACNGT 1 cut(s) 118
TaiI ACGT 3 cut(s) 425, 533, 797
TaqI TCGA 5 cut(s) 12, 62, 87, 191, 876
TasI AATT 4 cut(s) 18, 58, 471, 623
TatI WGTACW 2 cut(s) 287, 600
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 3 cut(s) 102, 193, 313
Tru1I TTAA 2 cut(s) 320, 413
Tru9I TTAA 2 cut(s) 320, 413
TscAI CASTG 1 cut(s) 577
TseFI GTSAC 1 cut(s) 27
Tsp45I GTSAC 1 cut(s) 27
TspDTI ATGAA 3 cut(s) 531, 545, 872
TspRI CASTG 1 cut(s) 577
XmiI GTMKAC 1 cut(s) 843
XspI CTAG 3 cut(s) 417, 609, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.