MD06G1201800.v1.1

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004430
Physical Location & Seq
Forward (+)
33505620 .. 33508240
2621 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1201800.v1.1.491

Sequence Viewer

Length: 915 bp
ATGGCGACGAACCTTCGAGAACACTTCAACAAGCCGGTCAACTCGCCGATCCCGAGCCACCCAGCTACAATCGGCGGTCAAGATCAGAGCTTGTCCGTCGTCATCTCGCCGGAGTGCCATGGATTCTGGTACGACGCCGTTCTAGTGGTTCCCGCAGTCGTTTTCGTGGTGTACTTGGCGTTTCAAGCCAAGAAGAACGTCATCAAGCTCTCCAATGGAAGATCATACATCATGATATCCTATTATGCCCTACTCTGGGTCGCCAGCCTTCTCAACCTCGCCTGGTGCGCTCTTCAGGCATGGCAGTGCTCAGGTGGGAAGGAGGTTGCTTGGAATGTGTTGTCATTGTTTACGGGTTCTGCAATGCTGGGATTGGAGATTAGCTTGGTGGGTTTCTTGCTCCAAGAGGATTATGCAAGTGGGGTTGAAGCTTTAGCTCATACTTTCTTGGTCTCTGGGATTGTTGTTGGTGTAGACATACTGCTTAAGGTCATCTATGTATTCGGGTTTGGGGTACCGCTGTTCACTGTTGGCATTGGAAGTACATATGGAGTGAAATGGGGCTTGTGGCTCATCCACAAATTATTGCTTACTGCAGTTTATGGCTTCATATTGTTTGTGCATTTCTCAAAGTGGAGAGAGAAGTTACCTCCTAGACCAGCATTCTACAACTACATCGTCATAATGTTTGTACTAAGTGCTTTGGCATCATTTGGTTGCGGGCTTGCTGGAATTGGAGCTGGTTTTGGAATCTGGTTGTACAATTTGACGGACATATGCTATCACACTCTTTATCTTCCGTTTCTGTACGTGATTTTTCTAGCGGATTTTTTCCAGGAAGAAAGTTTTCTCCTGGAGAATGCGTATTACTCAGAGATGAAGGATGCTGGGTTCTTTGATGCCGATTGGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

305

Amino Acids

33.86

Weight (kDa)

5.6

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_40 PF10160 38 - 291 8.7e-110 Predicted membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 514
AccB1I GGYRCC 1 cut(s) 514
AccI GTMKAC 1 cut(s) 474
AciI CCGC 5 cut(s) 75, 153, 518, 720, 824
AclWI GGATC 1 cut(s) 43
AcuI CTGAAG 1 cut(s) 278
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 7 cut(s) 131, 173, 516, 544, 693, 761, 809
AfiI CCNNNNNNNGG 2 cut(s) 255, 256
AflII CTTAAG 1 cut(s) 485
AgsI TTSAA 3 cut(s) 28, 185, 428
AjnI CCWGG 3 cut(s) 281, 834, 852
AloI GAACNNNNNNTCC 2 cut(s) 875, 907
AluBI AGCT 7 cut(s) 65, 90, 208, 384, 431, 437, 740
AluI AGCT 7 cut(s) 65, 90, 208, 384, 431, 437, 740
Alw21I GWGCWC 1 cut(s) 311
Alw26I GTCTC 1 cut(s) 457
AlwI GGATC 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 52
Asp700I GAANNNNTTC 1 cut(s) 846
Asp718I GGTACC 1 cut(s) 514
AspLEI GCGC 1 cut(s) 290
AvaI CYCGRG 1 cut(s) 52
BaeI ACNNNNGTAYC 2 cut(s) 506, 539
BanI GGYRCC 1 cut(s) 514
Bbv12I GWGCWC 1 cut(s) 311
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 3 cut(s) 283, 836, 854
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 3 cut(s) 143, 654, 821
BfmI CTRYAG 1 cut(s) 594
BfrI CTTAAG 1 cut(s) 485
Bme1390I CCNGG 3 cut(s) 283, 836, 854
BmeT110I CYCGRG 1 cut(s) 52
BmiI GGNNCC 2 cut(s) 150, 516
BmrFI CCNGG 3 cut(s) 283, 836, 854
BmsI GCATC 3 cut(s) 716, 874, 889
BpmI CTGGAG 1 cut(s) 875
Bpu10I CCTNAGC 1 cut(s) 310
BsaAI YACGTR 1 cut(s) 811
BsaHI GRCGYC 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 457
BsaJI CCNNGG 1 cut(s) 118
BsaXI ACNNNNNCTCC 2 cut(s) 848, 878
Bsc4I CCNNNNNNNGG 2 cut(s) 255, 256
Bse118I RCCGGY 1 cut(s) 34
Bse3DI GCAATG 1 cut(s) 369
BseBI CCWGG 3 cut(s) 283, 836, 854
BseDI CCNNGG 1 cut(s) 118
BseGI GGATG 2 cut(s) 573, 889
BseLI CCNNNNNNNGG 2 cut(s) 255, 256
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 2 cut(s) 324, 885
BseYI CCCAGC 3 cut(s) 61, 367, 887
BshNI GGYRCC 1 cut(s) 514
BsiHKAI GWGCWC 1 cut(s) 311
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 2 cut(s) 35, 110
BslI CCNNNNNNNGG 2 cut(s) 255, 256
BsmAI GTCTC 1 cut(s) 457
BsmI GAATGC 2 cut(s) 662, 865
Bso31I GGTCTC 1 cut(s) 457
BsoBI CYCGRG 1 cut(s) 52
Bsp1286I GDGCHC 1 cut(s) 311
Bsp1407I TGTACA 1 cut(s) 759
Bsp143I GATC 3 cut(s) 48, 82, 221
Bsp19I CCATGG 1 cut(s) 118
BspACI CCGC 5 cut(s) 75, 153, 518, 720, 824
BspCNI CTCAG 2 cut(s) 323, 884
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 2 cut(s) 150, 516
BspMAI CTGCAG 1 cut(s) 598
BspPI GGATC 1 cut(s) 43
BspQI GCTCTTC 1 cut(s) 297
BspT107I GGYRCC 1 cut(s) 514
BspTI CTTAAG 1 cut(s) 485
BspTNI GGTCTC 1 cut(s) 457
BsrDI GCAATG 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 759
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 3 cut(s) 48, 82, 221
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 118
Bst2UI CCWGG 3 cut(s) 283, 836, 854
Bst4CI ACNGT 1 cut(s) 529
Bst6I CTCTTC 1 cut(s) 297
BstACI GRCGYC 1 cut(s) 135
BstAFI CTTAAG 1 cut(s) 485
BstAUI TGTACA 1 cut(s) 759
BstBAI YACGTR 1 cut(s) 811
BstC8I GCNNGC 3 cut(s) 265, 722, 726
BstDEI CTNAG 3 cut(s) 310, 695, 871
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 2 cut(s) 573, 889
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 3 cut(s) 51, 85, 224
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 3 cut(s) 48, 82, 221
BstMWI GCNNNNNNNGC 3 cut(s) 185, 287, 296
BstNI CCWGG 3 cut(s) 283, 836, 854
BstSCI CCNGG 3 cut(s) 281, 834, 852
BstSFI CTRYAG 1 cut(s) 594
BtgI CCRYGG 1 cut(s) 118
BtsCI GGATG 2 cut(s) 573, 889
BtsI GCAGTG 1 cut(s) 311
BtsIMutI CAGTG 2 cut(s) 311, 525
Cac8I GCNNGC 3 cut(s) 265, 722, 726
CciI TCATGA 1 cut(s) 231
CfoI GCGC 1 cut(s) 290
Cfr10I RCCGGY 1 cut(s) 34
CseI GACGC 1 cut(s) 143
Csp6I GTAC 7 cut(s) 130, 172, 515, 543, 692, 760, 808
CviAII CATG 3 cut(s) 119, 232, 300
CviQI GTAC 7 cut(s) 130, 172, 515, 543, 692, 760, 808
DdeI CTNAG 3 cut(s) 310, 695, 871
DpnI GATC 3 cut(s) 50, 84, 223
DpnII GATC 3 cut(s) 48, 82, 221
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 118
Eco31I GGTCTC 1 cut(s) 457
Eco32I GATATC 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 278
Eco88I CYCGRG 1 cut(s) 52
EcoRII CCWGG 3 cut(s) 281, 834, 852
EcoRV GATATC 1 cut(s) 237
EcoT14I CCWWGG 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 3 cut(s) 122, 235, 303
FatI CATG 3 cut(s) 118, 231, 299
FauI CCCGC 2 cut(s) 160, 713
FauNDI CATATG 2 cut(s) 547, 776
FblI GTMKAC 1 cut(s) 474
FokI GGATG 2 cut(s) 560, 896
FspBI CTAG 3 cut(s) 143, 654, 821
GlaI GCGC 1 cut(s) 289
GsaI CCCAGC 3 cut(s) 65, 371, 891
GsuI CTGGAG 1 cut(s) 875
HapII CCGG 2 cut(s) 35, 110
HgaI GACGC 1 cut(s) 143
HhaI GCGC 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 3 cut(s) 122, 235, 303
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 429
HinfI GANTC 2 cut(s) 123, 750
HpaII CCGG 2 cut(s) 35, 110
Hpy166II GTNNAC 5 cut(s) 40, 172, 351, 475, 525
Hpy188I TCNGA 2 cut(s) 87, 874
Hpy188III TCNNGA 4 cut(s) 17, 52, 80, 232
Hpy8I GTNNAC 5 cut(s) 40, 172, 351, 475, 525
Hpy99I CGWCG 3 cut(s) 10, 101, 137
HpyAV CCTTC 4 cut(s) 23, 278, 313, 874
HpyCH4III ACNGT 1 cut(s) 529
HpyCH4IV ACGT 2 cut(s) 198, 810
HpyCH4V TGCA 4 cut(s) 362, 416, 596, 622
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 287, 296
HpyF3I CTNAG 3 cut(s) 310, 695, 871
HpySE526I ACGT 2 cut(s) 198, 810
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 3 cut(s) 122, 235, 303
HspAI GCGC 1 cut(s) 288
KpnI GGTACC 1 cut(s) 518
Kzo9I GATC 3 cut(s) 48, 82, 221
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 2 cut(s) 405, 737
LweI GCATC 3 cut(s) 716, 874, 889
MaeI CTAG 3 cut(s) 143, 654, 821
MaeII ACGT 2 cut(s) 198, 810
MaeIII GTNAC 1 cut(s) 645
MalI GATC 3 cut(s) 50, 84, 223
MboI GATC 3 cut(s) 48, 82, 221
MboII GAAGA 5 cut(s) 205, 231, 284, 788, 851
MhlI GDGCHC 1 cut(s) 311
MluCI AATT 3 cut(s) 581, 732, 763
MnlI CCTC 4 cut(s) 287, 316, 400, 660
MroXI GAANNNNTTC 1 cut(s) 846
MseI TTAA 1 cut(s) 486
MslI CAYNNNNRTG 1 cut(s) 304
MspA1I CMGCKG 1 cut(s) 520
MspCI CTTAAG 1 cut(s) 485
MspI CCGG 2 cut(s) 35, 110
MspR9I CCNGG 3 cut(s) 283, 836, 854
Mva1269I GAATGC 2 cut(s) 662, 865
MvaI CCWGG 3 cut(s) 283, 836, 854
MwoI GCNNNNNNNGC 3 cut(s) 185, 287, 296
NcoI CCATGG 1 cut(s) 118
NdeI CATATG 2 cut(s) 547, 776
NdeII GATC 3 cut(s) 48, 82, 221
NlaIII CATG 3 cut(s) 122, 235, 303
NlaIV GGNNCC 2 cut(s) 150, 516
PagI TCATGA 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PctI GAATGC 2 cut(s) 662, 865
PdmI GAANNNNTTC 1 cut(s) 846
PfeI GAWTC 2 cut(s) 123, 750
PfoI TCCNGGA 2 cut(s) 834, 852
Ppu21I YACGTR 1 cut(s) 811
Psp6I CCWGG 3 cut(s) 281, 834, 852
PspFI CCCAGC 3 cut(s) 61, 367, 887
PspGI CCWGG 3 cut(s) 281, 834, 852
PspN4I GGNNCC 2 cut(s) 150, 516
PstI CTGCAG 1 cut(s) 598
RsaI GTAC 7 cut(s) 131, 173, 516, 544, 693, 761, 809
RsaNI GTAC 7 cut(s) 130, 172, 515, 543, 692, 760, 808
RseI CAYNNNNRTG 1 cut(s) 304
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 486
Sau3AI GATC 3 cut(s) 48, 82, 221
ScrFI CCNGG 3 cut(s) 283, 836, 854
SduI GDGCHC 1 cut(s) 311
SfaNI GCATC 3 cut(s) 716, 874, 889
SfcI CTRYAG 1 cut(s) 594
SmiMI CAYNNNNRTG 1 cut(s) 304
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 3 cut(s) 581, 732, 763
SsiI CCGC 5 cut(s) 75, 153, 518, 720, 824
SspMI CTAG 3 cut(s) 143, 654, 821
StyD4I CCNGG 3 cut(s) 281, 834, 852
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 1 cut(s) 529
TaiI ACGT 2 cut(s) 201, 813
TaqI TCGA 1 cut(s) 16
TasI AATT 3 cut(s) 581, 732, 763
TatI WGTACW 4 cut(s) 171, 542, 691, 759
TfiI GAWTC 2 cut(s) 123, 750
Tru1I TTAA 1 cut(s) 486
Tru9I TTAA 1 cut(s) 486
TscAI CASTG 2 cut(s) 311, 532
TspDTI ATGAA 2 cut(s) 598, 893
TspGWI ACGGA 3 cut(s) 85, 785, 789
TspRI CASTG 2 cut(s) 311, 532
Vha464I CTTAAG 1 cut(s) 485
XmiI GTMKAC 1 cut(s) 474
XmnI GAANNNNTTC 1 cut(s) 846
XspI CTAG 3 cut(s) 143, 654, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.