FvH4_5g14000

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004430
Physical Location & Seq
Reverse (-)
7927344 .. 7929930
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14000.t2

Sequence Viewer

Length: 918 bp
ATGTCAACAACACCAAACCTTACCCAATACCTGAACGCCTCGGTCAACGCGCCGACGATCCCGAGCCTTCCCGCCACCGTCGACGGCGACGATCGGAGCTCTTCCACCTCATTAAACTGCCATGGCCTCTGGTACGACGCCGTCCTCGTCGTCTCTTCCCTCCTTTTCCTGCTCTACTTGGCCTTCCACGCCAAGAAGAACCTCAAGAAACTCTCTAATGGCAGCTCGTACATCATGATTTCCTATTATGCCCTCGTCTGGGTCGCCGCGCTTCTCAACCTCGCCTGGTCCGCTCTTCAGGGTTGGCAGTGCTCTGGTGGAAAGGCGGTGGCTTGGAATGTGCTGTGTTTGTTCACAGCTTCTGCGATGATGTGCTTGGAGATTAGTTTGGTGGCTTTTTTGCTCCAGGATAATTACACAAGTGGGGTTGATGCTTTGGCTCAGACTTTCCTTATCTCCGGGATCGTTGTGAGTGTGGATATGCTGCTCAAGGCAATCTATGTATTTGGATTTGGCGTCCCTCTCTTCATGGTTGGTATTGGAGGTACAACTGGAGCCTTGTGGGGCCTATGGTTCTTCCACAAATTGTTGCTCACTGCAGTTTATGGCTTCATATTGTTTGTGCATTTCTCTAAATGGAGAGAAAAGCTTCCACCCAGACCAGCCTTTTACAACTACATTCTCATAATGTTTGCGTTTGGTGCTGTGACATTGTTGGCTTGTGGGCTTGCTGGAATCGGGGCTGGTTTTGGCATTTGGTTGTACAATTTGACAGATATATGTTACCACTCTCTCTATCTTCCATTTCTATATGTAACCTTTCTGGCAGATTTTTTCCAGGAAGAAGAATTTCTTCTGGATAATGCATATTACTCTGAGATGAAGGATGCAGGGTTCTTTGATGCTGATTGGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

306

Amino Acids

33.82

Weight (kDa)

5.08

Isoelectric Point (pI)

32.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_40 PF10160 39 - 280 2.7e-100 Predicted membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 293
AccI GTMKAC 1 cut(s) 81
AccII CGCG 2 cut(s) 50, 269
AciI CCGC 4 cut(s) 72, 267, 291, 326
AclWI GGATC 2 cut(s) 52, 470
AcsI RAATTY 1 cut(s) 848
AcuI CTGAAG 1 cut(s) 281
AcyI GRCGYC 2 cut(s) 138, 516
AfaI GTAC 4 cut(s) 134, 230, 547, 764
AfiI CCNNNNNNNGG 2 cut(s) 258, 259
AjnI CCWGG 3 cut(s) 284, 405, 837
AloI GAACNNNNNNTCC 2 cut(s) 878, 910
AluBI AGCT 4 cut(s) 99, 225, 359, 649
AluI AGCT 4 cut(s) 99, 225, 359, 649
Alw21I GWGCWC 2 cut(s) 101, 314
Alw26I GTCTC 1 cut(s) 157
AlwI GGATC 2 cut(s) 52, 470
AlwNI CAGNNNCTG 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 61
AoxI GGCC 3 cut(s) 124, 180, 565
ApeKI GCWGC 2 cut(s) 222, 484
ApoI RAATTY 1 cut(s) 848
Asp700I GAANNNNTTC 3 cut(s) 648, 849, 852
AspLEI GCGC 2 cut(s) 52, 271
AspS9I GGNCC 2 cut(s) 288, 565
AsuC2I CCSGG 1 cut(s) 460
AvaI CYCGRG 1 cut(s) 61
AvaII GGWCC 1 cut(s) 288
BanII GRGCYC 1 cut(s) 101
Bbv12I GWGCWC 2 cut(s) 101, 314
BbvI GCAGC 2 cut(s) 234, 471
BceAI ACGGC 2 cut(s) 100, 125
BciT130I CCWGG 3 cut(s) 286, 407, 839
BcnI CCSGG 1 cut(s) 460
BcoDI GTCTC 1 cut(s) 157
BfmI CTRYAG 1 cut(s) 597
BisI GCNGC 3 cut(s) 223, 267, 485
BlsI GCNGC 3 cut(s) 224, 268, 486
Bme1390I CCNGG 4 cut(s) 286, 407, 460, 839
Bme18I GGWCC 1 cut(s) 288
BmeT110I CYCGRG 1 cut(s) 61
BmgT120I GGNCC 2 cut(s) 288, 565
BmiI GGNNCC 2 cut(s) 556, 566
BmrFI CCNGG 4 cut(s) 286, 407, 460, 839
BmsI GCATC 3 cut(s) 421, 877, 892
BpmI CTGGAG 2 cut(s) 389, 573
BpuEI CTTGAG 2 cut(s) 188, 473
BpuMI CCSGG 1 cut(s) 460
BsaHI GRCGYC 2 cut(s) 138, 516
BsaJI CCNNGG 2 cut(s) 39, 121
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 259
Bse1I ACTGG 1 cut(s) 556
BseBI CCWGG 3 cut(s) 286, 407, 839
BseDI CCNNGG 2 cut(s) 39, 121
BseGI GGATG 1 cut(s) 892
BseLI CCNNNNNNNGG 2 cut(s) 258, 259
BseMII CTCAG 2 cut(s) 455, 867
BseNI ACTGG 1 cut(s) 556
BseXI GCAGC 2 cut(s) 234, 471
Bsh1236I CGCG 2 cut(s) 50, 269
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 3 cut(s) 126, 182, 567
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 2 cut(s) 101, 314
BsiHKCI CYCGRG 1 cut(s) 61
BsiSI CCGG 1 cut(s) 459
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 2 cut(s) 258, 259
BsmAI GTCTC 1 cut(s) 157
BsmBI CGTCTC 1 cut(s) 157
BsmFI GGGAC 1 cut(s) 503
BsnI GGCC 3 cut(s) 126, 182, 567
BsoBI CYCGRG 1 cut(s) 61
Bsp1286I GDGCHC 2 cut(s) 101, 314
Bsp1407I TGTACA 1 cut(s) 762
Bsp143I GATC 3 cut(s) 57, 91, 462
Bsp19I CCATGG 1 cut(s) 121
BspACI CCGC 4 cut(s) 72, 267, 291, 326
BspANI GGCC 3 cut(s) 126, 182, 567
BspCNI CTCAG 2 cut(s) 454, 868
BspFNI CGCG 2 cut(s) 50, 269
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 556, 566
BspMAI CTGCAG 1 cut(s) 601
BspPI GGATC 2 cut(s) 52, 470
BspQI GCTCTTC 2 cut(s) 106, 300
BsrBI CCGCTC 1 cut(s) 293
BsrGI TGTACA 1 cut(s) 762
BsrI ACTGG 1 cut(s) 556
BssECI CCNNGG 2 cut(s) 39, 121
BssMI GATC 3 cut(s) 57, 91, 462
BssNI GRCGYC 2 cut(s) 138, 516
BssT1I CCWWGG 1 cut(s) 121
Bst2UI CCWGG 3 cut(s) 286, 407, 839
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 4 cut(s) 106, 160, 300, 530
BstACI GRCGYC 2 cut(s) 138, 516
BstAUI TGTACA 1 cut(s) 762
BstC8I GCNNGC 1 cut(s) 729
BstDEI CTNAG 2 cut(s) 441, 876
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 1 cut(s) 892
BstFNI CGCG 2 cut(s) 50, 269
BstHHI GCGC 2 cut(s) 52, 271
BstKTI GATC 3 cut(s) 60, 94, 465
BstMAI GTCTC 1 cut(s) 157
BstMBI GATC 3 cut(s) 57, 91, 462
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 3 cut(s) 188, 290, 701
BstNI CCWGG 3 cut(s) 286, 407, 839
BstSCI CCNGG 4 cut(s) 284, 405, 458, 837
BstSFI CTRYAG 1 cut(s) 597
BstUI CGCG 2 cut(s) 50, 269
BstV1I GCAGC 2 cut(s) 234, 471
BsuRI GGCC 3 cut(s) 126, 182, 567
BtgI CCRYGG 1 cut(s) 121
BtgZI GCGATG 1 cut(s) 380
BtsCI GGATG 1 cut(s) 892
BtsI GCAGTG 2 cut(s) 314, 594
BtsIMutI CAGTG 2 cut(s) 314, 594
Cac8I GCNNGC 1 cut(s) 729
CaiI CAGNNNCTG 1 cut(s) 362
CciI TCATGA 1 cut(s) 234
CfoI GCGC 2 cut(s) 52, 271
Cfr13I GGNCC 2 cut(s) 288, 565
CseI GACGC 2 cut(s) 146, 505
Csp6I GTAC 4 cut(s) 133, 229, 546, 763
CviAII CATG 3 cut(s) 122, 235, 529
CviQI GTAC 4 cut(s) 133, 229, 546, 763
DdeI CTNAG 2 cut(s) 441, 876
DpnI GATC 3 cut(s) 59, 93, 464
DpnII GATC 3 cut(s) 57, 91, 462
Eam1104I CTCTTC 4 cut(s) 106, 160, 300, 530
EarI CTCTTC 4 cut(s) 106, 160, 300, 530
Ecl136II GAGCTC 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 101
Eco47I GGWCC 1 cut(s) 288
Eco53kI GAGCTC 1 cut(s) 99
Eco57I CTGAAG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 61
EcoICRI GAGCTC 1 cut(s) 99
EcoO109I RGGNCCY 1 cut(s) 565
EcoRII CCWGG 3 cut(s) 284, 405, 837
EcoT14I CCWWGG 1 cut(s) 121
EcoT22I ATGCAT 1 cut(s) 868
EcoT38I GRGCYC 1 cut(s) 101
ErhI CCWWGG 1 cut(s) 121
Esp3I CGTCTC 1 cut(s) 157
FaeI CATG 3 cut(s) 125, 238, 532
FalI AAGNNNNNCTT 2 cut(s) 837, 869
FaqI GGGAC 1 cut(s) 503
FatI CATG 3 cut(s) 121, 234, 528
FauI CCCGC 1 cut(s) 79
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 3 cut(s) 223, 267, 485
FokI GGATG 1 cut(s) 899
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 3 cut(s) 223, 267, 485
GlaI GCGC 2 cut(s) 51, 270
GluI GCNGC 3 cut(s) 223, 267, 485
GsuI CTGGAG 2 cut(s) 389, 573
HaeIII GGCC 3 cut(s) 126, 182, 567
HapII CCGG 1 cut(s) 459
HgaI GACGC 2 cut(s) 146, 505
HhaI GCGC 2 cut(s) 52, 271
Hin1I GRCGYC 2 cut(s) 138, 516
Hin1II CATG 3 cut(s) 125, 238, 532
Hin6I GCGC 2 cut(s) 50, 269
HinP1I GCGC 2 cut(s) 50, 269
HincII GTYRAC 3 cut(s) 6, 46, 82
HindII GTYRAC 3 cut(s) 6, 46, 82
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 1 cut(s) 735
HpaII CCGG 1 cut(s) 459
Hpy166II GTNNAC 4 cut(s) 6, 46, 82, 354
Hpy188I TCNGA 3 cut(s) 96, 444, 877
Hpy188III TCNNGA 4 cut(s) 61, 205, 235, 857
Hpy8I GTNNAC 4 cut(s) 6, 46, 82, 354
Hpy99I CGWCG 6 cut(s) 58, 83, 86, 92, 140, 152
HpyAV CCTTC 3 cut(s) 77, 193, 877
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4V TGCA 4 cut(s) 599, 625, 866, 890
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 290, 701
HpyF3I CTNAG 2 cut(s) 441, 876
Hsp92I GRCGYC 2 cut(s) 138, 516
Hsp92II CATG 3 cut(s) 125, 238, 532
HspAI GCGC 2 cut(s) 50, 269
Kzo9I GATC 3 cut(s) 57, 91, 462
LguI GCTCTTC 2 cut(s) 106, 300
LmnI GCTCC 3 cut(s) 96, 408, 554
Lsp1109I GCAGC 2 cut(s) 234, 471
LweI GCATC 3 cut(s) 421, 877, 892
MaeIII GTNAC 3 cut(s) 706, 782, 814
MalI GATC 3 cut(s) 59, 93, 464
MbiI CCGCTC 1 cut(s) 293
MboI GATC 3 cut(s) 57, 91, 462
MhlI GDGCHC 2 cut(s) 101, 314
MluCI AATT 4 cut(s) 412, 584, 766, 848
Mph1103I ATGCAT 1 cut(s) 868
MroXI GAANNNNTTC 3 cut(s) 648, 849, 852
MseI TTAA 1 cut(s) 113
MspI CCGG 1 cut(s) 459
MspR9I CCNGG 4 cut(s) 286, 407, 460, 839
MvaI CCWGG 3 cut(s) 286, 407, 839
MvnI CGCG 2 cut(s) 50, 269
MwoI GCNNNNNNNGC 3 cut(s) 188, 290, 701
NciI CCSGG 1 cut(s) 460
NcoI CCATGG 1 cut(s) 121
NdeII GATC 3 cut(s) 57, 91, 462
NlaIII CATG 3 cut(s) 125, 238, 532
NlaIV GGNNCC 2 cut(s) 556, 566
NmuCI GTSAC 1 cut(s) 706
NsiI ATGCAT 1 cut(s) 868
PagI TCATGA 1 cut(s) 234
PciSI GCTCTTC 2 cut(s) 106, 300
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 144
PdmI GAANNNNTTC 3 cut(s) 648, 849, 852
PfeI GAWTC 1 cut(s) 735
PflFI GACNNNGTC 1 cut(s) 140
PfoI TCCNGGA 3 cut(s) 405, 458, 837
PkrI GCNGC 3 cut(s) 224, 268, 486
Ple19I CGATCG 1 cut(s) 94
Psp124BI GAGCTC 1 cut(s) 101
Psp6I CCWGG 3 cut(s) 284, 405, 837
PspGI CCWGG 3 cut(s) 284, 405, 837
PspN4I GGNNCC 2 cut(s) 556, 566
PspPI GGNCC 2 cut(s) 288, 565
PstI CTGCAG 1 cut(s) 601
PstNI CAGNNNCTG 1 cut(s) 362
PsyI GACNNNGTC 1 cut(s) 140
PvuI CGATCG 1 cut(s) 94
RsaI GTAC 4 cut(s) 134, 230, 547, 764
RsaNI GTAC 4 cut(s) 133, 229, 546, 763
SacI GAGCTC 1 cut(s) 101
SalI GTCGAC 1 cut(s) 80
SapI GCTCTTC 2 cut(s) 106, 300
SaqAI TTAA 1 cut(s) 113
SatI GCNGC 3 cut(s) 223, 267, 485
Sau3AI GATC 3 cut(s) 57, 91, 462
Sau96I GGNCC 2 cut(s) 288, 565
ScrFI CCNGG 4 cut(s) 286, 407, 460, 839
SduI GDGCHC 2 cut(s) 101, 314
SfaNI GCATC 3 cut(s) 421, 877, 892
SfcI CTRYAG 1 cut(s) 597
SgrDI CGTCGACG 1 cut(s) 80
SinI GGWCC 1 cut(s) 288
SmlI CTYRAG 2 cut(s) 203, 488
SmoI CTYRAG 2 cut(s) 203, 488
Sse9I AATT 4 cut(s) 412, 584, 766, 848
SsiI CCGC 4 cut(s) 72, 267, 291, 326
SstI GAGCTC 1 cut(s) 101
StyD4I CCNGG 4 cut(s) 284, 405, 458, 837
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 1 cut(s) 79
TaqI TCGA 1 cut(s) 81
TaqII GACCGA 1 cut(s) 31
TasI AATT 4 cut(s) 412, 584, 766, 848
TatI WGTACW 1 cut(s) 762
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 1 cut(s) 735
Tru1I TTAA 1 cut(s) 113
Tru9I TTAA 1 cut(s) 113
TscAI CASTG 2 cut(s) 314, 601
TseFI GTSAC 1 cut(s) 706
TseI GCWGC 2 cut(s) 222, 484
Tsp45I GTSAC 1 cut(s) 706
TspDTI ATGAA 3 cut(s) 517, 601, 896
TspRI CASTG 2 cut(s) 314, 601
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 288
XapI RAATTY 1 cut(s) 848
XmiI GTMKAC 1 cut(s) 81
XmnI GAANNNNTTC 3 cut(s) 648, 849, 852
Zsp2I ATGCAT 1 cut(s) 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.