Rh3BG048600

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0004430
Physical Location & Seq
Reverse (-)
3219558 .. 3222716
3159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG048600.1

Sequence Viewer

Length: 780 bp
ATGGCAGCTCCAGGTCCAGGAGAAGCTGCGTCGCGACCTCTGCTTCCAATTCTACTTCCCAAGGGCACAAATTCAACCCTAGGCGAAGCGATTTCGGGTCCCAGACTCTACAGCTGGCTCGGCCAGTGCCATGGGTTCTGGCACAATTTGGCCCTGATCACGTCCTCTCTTCTCTTTGCTCTCTACTTGGCCTATCAATCAAGGAAGAGCTTTTCCAAGCTCTATCATGGCCGCTCGTATATTATGATGGCCTACTATGGCTGCCTTTGGCTCGTTAGCTTGCTCAATCTCGCTTGGTGCTTTCTTCAGGCATGGGAATGCACACCTGATAAAGAAATGGCATGGAATGTCTTATCTTTGTTCACAACTTCCGGAATGTTATTTTTGGAAGTAAGCTTGCTGGCATTTTTACTCCAAGGGAATTATGCAAGTGGACTGGAAGCGTTGACACGAACTTTTGTTATCTCAGGGATTCTCGTTGGTTTGGATTTACTTCTGAAGGCAATTTATATGTTTGGATTTGGAATTCCGCTGTTTGTCGACGACACTGATCATACACATCAGATGAAGTGGAACTTGTGGTCTGTCCATAGGCTAGTACTAACTGCAGTTTATGGTTTCATATTGTTCATGTACCATTCCAAGTGGAGAGAAAGGCTACCCGCGAGACCTGCATTCTACAATTATACAGCTATCATGTTTCTGATGAATGTACTAGCAATGTTTGCTTGTGGACTTACAGGAAATGGAGGAGCTGGATTTGGGTTTTGGTATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

259

Amino Acids

29.15

Weight (kDa)

8.77

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_40 PF10160 42 - 258 4.3e-96 Predicted membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 679
AccBSI CCGCTC 1 cut(s) 234
AccI GTMKAC 1 cut(s) 540
AccII CGCG 2 cut(s) 34, 665
AccIII TCCGGA 1 cut(s) 371
AciI CCGC 3 cut(s) 232, 530, 663
AcoI YGGCCR 2 cut(s) 121, 229
AcsI RAATTY 2 cut(s) 70, 525
AcuI CTGAAG 2 cut(s) 290, 518
AfaI GTAC 3 cut(s) 600, 635, 714
AfiI CCNNNNNNNGG 1 cut(s) 17
AgsI TTSAA 1 cut(s) 75
AjiI CACGTC 1 cut(s) 162
AjnI CCWGG 2 cut(s) 10, 16
AluBI AGCT 9 cut(s) 8, 26, 114, 210, 220, 279, 396, 692, 755
AluI AGCT 9 cut(s) 8, 26, 114, 210, 220, 279, 396, 692, 755
Alw26I GTCTC 1 cut(s) 661
Aor13HI TCCGGA 1 cut(s) 371
AoxI GGCC 5 cut(s) 121, 150, 189, 229, 249
ApeKI GCWGC 3 cut(s) 5, 26, 261
ApoI RAATTY 2 cut(s) 70, 525
AspA2I CCTAGG 1 cut(s) 79
AspS9I GGNCC 3 cut(s) 14, 98, 151
AvaII GGWCC 2 cut(s) 14, 98
AvrII CCTAGG 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 68
BbvI GCAGC 3 cut(s) 13, 17, 248
BccI CCATC 1 cut(s) 241
BciT130I CCWGG 2 cut(s) 12, 18
BclI TGATCA 2 cut(s) 156, 550
BcoDI GTCTC 1 cut(s) 661
BfaI CTAG 3 cut(s) 80, 596, 716
BfmI CTRYAG 2 cut(s) 109, 606
BfuAI ACCTGC 1 cut(s) 679
BisI GCNGC 4 cut(s) 6, 27, 232, 262
BlnI CCTAGG 1 cut(s) 79
BlsI GCNGC 4 cut(s) 7, 28, 233, 263
BmcAI AGTACT 1 cut(s) 600
Bme1390I CCNGG 2 cut(s) 12, 18
Bme18I GGWCC 2 cut(s) 14, 98
BmgBI CACGTC 1 cut(s) 162
BmgT120I GGNCC 3 cut(s) 14, 98, 151
BmiI GGNNCC 2 cut(s) 99, 100
BmrFI CCNGG 2 cut(s) 12, 18
BsaI GGTCTC 1 cut(s) 661
BsaJI CCNNGG 4 cut(s) 60, 79, 130, 415
BsaWI WCCGGW 1 cut(s) 371
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse1I ACTGG 2 cut(s) 124, 441
Bse3DI GCAATG 1 cut(s) 726
BseAI TCCGGA 1 cut(s) 371
BseBI CCWGG 2 cut(s) 12, 18
BseDI CCNNGG 4 cut(s) 60, 79, 130, 415
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMI GCAATG 1 cut(s) 726
BseMII CTCAG 1 cut(s) 480
BseNI ACTGG 2 cut(s) 124, 441
BseRI GAGGAG 1 cut(s) 765
BseSI GKGCMC 1 cut(s) 68
BseXI GCAGC 3 cut(s) 13, 17, 248
Bsh1236I CGCG 2 cut(s) 34, 665
BshFI GGCC 5 cut(s) 123, 152, 191, 231, 251
BsiSI CCGG 1 cut(s) 372
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 661
BsmFI GGGAC 1 cut(s) 84
BsmI GAATGC 2 cut(s) 323, 674
BsnI GGCC 5 cut(s) 123, 152, 191, 231, 251
Bso31I GGTCTC 1 cut(s) 661
Bsp1286I GDGCHC 1 cut(s) 68
Bsp13I TCCGGA 1 cut(s) 371
Bsp143I GATC 2 cut(s) 156, 550
Bsp19I CCATGG 1 cut(s) 130
Bsp68I TCGCGA 1 cut(s) 34
BspACI CCGC 3 cut(s) 232, 530, 663
BspANI GGCC 5 cut(s) 123, 152, 191, 231, 251
BspCNI CTCAG 1 cut(s) 479
BspEI TCCGGA 1 cut(s) 371
BspFNI CGCG 2 cut(s) 34, 665
BspLI GGNNCC 2 cut(s) 99, 100
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 1 cut(s) 679
BspQI GCTCTTC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 661
BsrBI CCGCTC 1 cut(s) 234
BsrDI GCAATG 1 cut(s) 726
BsrI ACTGG 2 cut(s) 124, 441
BssECI CCNNGG 4 cut(s) 60, 79, 130, 415
BssMI GATC 2 cut(s) 156, 550
BssT1I CCWWGG 4 cut(s) 60, 79, 130, 415
Bst2UI CCWGG 2 cut(s) 12, 18
Bst6I CTCTTC 2 cut(s) 174, 200
BstAPI GCANNNNNTGC 1 cut(s) 725
BstC8I GCNNGC 4 cut(s) 116, 281, 398, 402
BstDEI CTNAG 1 cut(s) 466
BstDSI CCRYGG 1 cut(s) 130
BstFNI CGCG 2 cut(s) 34, 665
BstKTI GATC 2 cut(s) 159, 553
BstMAI GTCTC 1 cut(s) 661
BstMBI GATC 2 cut(s) 156, 550
BstMWI GCNNNNNNNGC 4 cut(s) 40, 120, 671, 725
BstNI CCWGG 2 cut(s) 12, 18
BstSCI CCNGG 2 cut(s) 10, 16
BstSFI CTRYAG 2 cut(s) 109, 606
BstSLI GKGCMC 1 cut(s) 68
BstUI CGCG 2 cut(s) 34, 665
BstV1I GCAGC 3 cut(s) 13, 17, 248
BstXI CCANNNNNNTGG 1 cut(s) 131
BsuRI GGCC 5 cut(s) 123, 152, 191, 231, 251
BtgI CCRYGG 1 cut(s) 130
BtrI CACGTC 1 cut(s) 162
BtsIMutI CAGTG 2 cut(s) 131, 546
BtuMI TCGCGA 1 cut(s) 34
BveI ACCTGC 1 cut(s) 679
Cac8I GCNNGC 4 cut(s) 116, 281, 398, 402
Cfr13I GGNCC 3 cut(s) 14, 98, 151
CseI GACGC 1 cut(s) 18
Csp6I GTAC 3 cut(s) 599, 634, 713
CviAII CATG 6 cut(s) 131, 227, 312, 342, 631, 697
CviQI GTAC 3 cut(s) 599, 634, 713
DdeI CTNAG 1 cut(s) 466
DpnI GATC 2 cut(s) 158, 552
DpnII GATC 2 cut(s) 156, 550
EaeI YGGCCR 2 cut(s) 121, 229
Eam1104I CTCTTC 2 cut(s) 174, 200
EarI CTCTTC 2 cut(s) 174, 200
Eco130I CCWWGG 4 cut(s) 60, 79, 130, 415
Eco31I GGTCTC 1 cut(s) 661
Eco47I GGWCC 2 cut(s) 14, 98
Eco57I CTGAAG 2 cut(s) 290, 518
EcoO109I RGGNCCY 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 525
EcoRII CCWGG 2 cut(s) 10, 16
EcoT14I CCWWGG 4 cut(s) 60, 79, 130, 415
EcoT22I ATGCAT 1 cut(s) 778
ErhI CCWWGG 4 cut(s) 60, 79, 130, 415
FaeI CATG 6 cut(s) 134, 230, 315, 345, 634, 700
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 1 cut(s) 84
FatI CATG 6 cut(s) 130, 226, 311, 341, 630, 696
FauI CCCGC 1 cut(s) 670
FbaI TGATCA 2 cut(s) 156, 550
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 4 cut(s) 6, 27, 232, 262
Fsp4HI GCNGC 4 cut(s) 6, 27, 232, 262
FspBI CTAG 3 cut(s) 80, 596, 716
GluI GCNGC 4 cut(s) 6, 27, 232, 262
HaeIII GGCC 5 cut(s) 123, 152, 191, 231, 251
HapII CCGG 1 cut(s) 372
HgaI GACGC 1 cut(s) 18
Hin1II CATG 6 cut(s) 134, 230, 315, 345, 634, 700
HincII GTYRAC 2 cut(s) 447, 541
HindII GTYRAC 2 cut(s) 447, 541
HindIII AAGCTT 1 cut(s) 394
HinfI GANTC 2 cut(s) 105, 472
HpaII CCGG 1 cut(s) 372
Hpy166II GTNNAC 5 cut(s) 363, 434, 447, 541, 734
Hpy188I TCNGA 3 cut(s) 498, 564, 705
Hpy188III TCNNGA 2 cut(s) 33, 372
Hpy8I GTNNAC 5 cut(s) 363, 434, 447, 541, 734
Hpy99I CGWCG 2 cut(s) 34, 545
HpyAV CCTTC 1 cut(s) 493
HpyCH4IV ACGT 1 cut(s) 161
HpyCH4V TGCA 5 cut(s) 321, 428, 608, 674, 776
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 120, 671, 725
HpyF3I CTNAG 1 cut(s) 466
HpySE526I ACGT 1 cut(s) 161
Hsp92II CATG 6 cut(s) 134, 230, 315, 345, 634, 700
KflI GGGWCCC 1 cut(s) 98
Kpn2I TCCGGA 1 cut(s) 371
Ksp22I TGATCA 2 cut(s) 156, 550
Kzo9I GATC 2 cut(s) 156, 550
LguI GCTCTTC 1 cut(s) 200
LmnI GCTCC 2 cut(s) 13, 752
Lsp1109I GCAGC 3 cut(s) 13, 17, 248
MaeI CTAG 3 cut(s) 80, 596, 716
MaeII ACGT 1 cut(s) 161
MalI GATC 2 cut(s) 158, 552
MbiI CCGCTC 1 cut(s) 234
MboI GATC 2 cut(s) 156, 550
MboII GAAGA 3 cut(s) 161, 217, 296
MhlI GDGCHC 1 cut(s) 68
MluCI AATT 7 cut(s) 48, 70, 145, 421, 504, 525, 682
MlyI GAGTC 1 cut(s) 99
MnlI CCTC 3 cut(s) 48, 175, 743
Mph1103I ATGCAT 1 cut(s) 778
MroI TCCGGA 1 cut(s) 371
MslI CAYNNNNRTG 1 cut(s) 316
MspA1I CMGCKG 2 cut(s) 114, 532
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 2 cut(s) 12, 18
Mva1269I GAATGC 2 cut(s) 323, 674
MvaI CCWGG 2 cut(s) 12, 18
MvnI CGCG 2 cut(s) 34, 665
MwoI GCNNNNNNNGC 4 cut(s) 40, 120, 671, 725
NcoI CCATGG 1 cut(s) 130
NdeII GATC 2 cut(s) 156, 550
NlaIII CATG 6 cut(s) 134, 230, 315, 345, 634, 700
NlaIV GGNNCC 2 cut(s) 99, 100
NmeAIII GCCGAG 1 cut(s) 99
NruI TCGCGA 1 cut(s) 34
NsiI ATGCAT 1 cut(s) 778
PciSI GCTCTTC 1 cut(s) 200
PctI GAATGC 2 cut(s) 323, 674
PfeI GAWTC 1 cut(s) 472
PfoI TCCNGGA 1 cut(s) 16
PkrI GCNGC 4 cut(s) 7, 28, 233, 263
PleI GAGTC 1 cut(s) 99
PpsI GAGTC 1 cut(s) 99
PpuMI RGGWCCY 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 10, 16
PspGI CCWGG 2 cut(s) 10, 16
PspN4I GGNNCC 2 cut(s) 99, 100
PspPI GGNCC 3 cut(s) 14, 98, 151
PspPPI RGGWCCY 1 cut(s) 98
PstI CTGCAG 1 cut(s) 610
PvuII CAGCTG 1 cut(s) 114
RruI TCGCGA 1 cut(s) 34
RsaI GTAC 3 cut(s) 600, 635, 714
RsaNI GTAC 3 cut(s) 599, 634, 713
RseI CAYNNNNRTG 1 cut(s) 316
SalI GTCGAC 1 cut(s) 539
SapI GCTCTTC 1 cut(s) 200
SatI GCNGC 4 cut(s) 6, 27, 232, 262
Sau3AI GATC 2 cut(s) 156, 550
Sau96I GGNCC 3 cut(s) 14, 98, 151
ScaI AGTACT 1 cut(s) 600
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 2 cut(s) 12, 18
SduI GDGCHC 1 cut(s) 68
SfcI CTRYAG 2 cut(s) 109, 606
SinI GGWCC 2 cut(s) 14, 98
SmiMI CAYNNNNRTG 1 cut(s) 316
Sse9I AATT 7 cut(s) 48, 70, 145, 421, 504, 525, 682
SsiI CCGC 3 cut(s) 232, 530, 663
SspMI CTAG 3 cut(s) 80, 596, 716
StyD4I CCNGG 2 cut(s) 10, 16
StyI CCWWGG 4 cut(s) 60, 79, 130, 415
TaiI ACGT 1 cut(s) 164
TaqI TCGA 1 cut(s) 540
TasI AATT 7 cut(s) 48, 70, 145, 421, 504, 525, 682
TatI WGTACW 2 cut(s) 598, 712
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 1 cut(s) 472
TscAI CASTG 2 cut(s) 131, 553
TseI GCWGC 3 cut(s) 5, 26, 261
TspDTI ATGAA 4 cut(s) 581, 610, 619, 722
TspRI CASTG 2 cut(s) 131, 553
VpaK11BI GGWCC 2 cut(s) 14, 98
XapI RAATTY 2 cut(s) 70, 525
XmaJI CCTAGG 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 540
XspI CTAG 3 cut(s) 80, 596, 716
ZrmI AGTACT 1 cut(s) 600
Zsp2I ATGCAT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.