Rw3G003620

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0004430
Physical Location & Seq
Reverse (-)
3065046 .. 3067399
2354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G003620.1

Sequence Viewer

Length: 954 bp
ATGGCAGCTCCAGGTCCAGGAGAAGCTGCGTCGCGACCTCTGCTTCCAATTCTACTTCCCAAGGGCACAAATTCAACCCTAGGCGAAGCGATTTCGGGTCCCAGACTCTACAGCTGGCTCGGCCAGTGCCATGGGTTCTGGCACAATTTGGCCCTGATCACGTCCTCGCTTCTCTTTACTCTATACTTGGCCTATCAATCAAGGAAGAGCTTTTCGAAGCTCTATCATGGCCGCTCGTATATTATGATGGCCTACTATGGCTGCCTTGGCTCGTTAGCTTGCTCAATCTCGCTTGGTGCTTTCTTCAGGTTCAAAAATTTTCCTACTTTTGAAGCATGGGAATGCACACCTGATAAAGAAATGGCATGGAATGTCTTATCTTTGTTCACAACTTCCGGAATGTTATTTTTGGAAGTAAGCTTGCTGGCATTTTTACTCCAAGGGAATTATGCAAGTGGACTGGAAGCGTTGACACGAACTTTTGTTATCTCAGGGATCCTCGTTGGTTTGGATTTACTTCTGAAGGCAATTTATATGTTTGGATTTGGAATTCCGCTGTTTGTCGACGACACTGATCATACACATCAGATGAAGTGGAACTTGTGGTCTGTCCATAGGCTAGTACTAACTGCAGTTTATGGTTTCATATTGTTCATGTACCATTCCAAGTGGAGAGAAAGGCTACCCGCGAGACCTGCATTCTACAATTATACAGCTATTATGTTTCTGATGAATGTACTAGCAATGTTTGCTTGTGGACTTACAGGAAATGGAGGAGCTGGATTTGGGTTTTGGTTATATGGCGCCACTATTGTCTGTTACCATGCCTTCTACCTTCCTCTTTTATATGTAACATTTCTGGCAGACTTCTTCCAGGAGGATGATTTACACTTGGAGAATGTATATTATTCAGAGATGAAAGATGCTGGTTTCTTTGATGCTGATTGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

317

Amino Acids

35.88

Weight (kDa)

6.13

Isoelectric Point (pI)

34.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_40 PF10160 42 - 304 1.1e-107 Predicted membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 703
AccB1I GGYRCC 1 cut(s) 803
AccBSI CCGCTC 1 cut(s) 234
AccI GTMKAC 1 cut(s) 564
AccII CGCG 2 cut(s) 34, 689
AccIII TCCGGA 1 cut(s) 395
AciI CCGC 3 cut(s) 232, 554, 687
AclWI GGATC 2 cut(s) 490, 503
AcoI YGGCCR 2 cut(s) 121, 229
AcsI RAATTY 3 cut(s) 70, 316, 549
AcuI CTGAAG 2 cut(s) 289, 542
AcyI GRCGYC 1 cut(s) 804
AfaI GTAC 3 cut(s) 624, 659, 738
AfiI CCNNNNNNNGG 1 cut(s) 17
AgsI TTSAA 3 cut(s) 75, 313, 332
AjiI CACGTC 1 cut(s) 162
AjnI CCWGG 3 cut(s) 10, 16, 873
AluBI AGCT 9 cut(s) 8, 26, 114, 210, 220, 278, 420, 716, 779
AluI AGCT 9 cut(s) 8, 26, 114, 210, 220, 278, 420, 716, 779
Alw26I GTCTC 1 cut(s) 685
AlwI GGATC 2 cut(s) 490, 503
Aor13HI TCCGGA 1 cut(s) 395
AoxI GGCC 5 cut(s) 121, 150, 189, 229, 249
ApeKI GCWGC 3 cut(s) 5, 26, 261
ApoI RAATTY 3 cut(s) 70, 316, 549
AspA2I CCTAGG 1 cut(s) 79
AspLEI GCGC 1 cut(s) 806
AspS9I GGNCC 3 cut(s) 14, 98, 151
AsuII TTCGAA 1 cut(s) 215
AvaII GGWCC 2 cut(s) 14, 98
AvrII CCTAGG 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 68
BamHI GGATCC 1 cut(s) 495
BanI GGYRCC 1 cut(s) 803
BbvI GCAGC 3 cut(s) 13, 17, 248
BccI CCATC 1 cut(s) 241
BciT130I CCWGG 3 cut(s) 12, 18, 875
BclI TGATCA 2 cut(s) 156, 574
BcoDI GTCTC 1 cut(s) 685
BfaI CTAG 3 cut(s) 80, 620, 740
BfmI CTRYAG 2 cut(s) 109, 630
BfoI RGCGCY 1 cut(s) 807
BfuAI ACCTGC 1 cut(s) 703
BisI GCNGC 4 cut(s) 6, 27, 232, 262
BlnI CCTAGG 1 cut(s) 79
BlsI GCNGC 4 cut(s) 7, 28, 233, 263
BmcAI AGTACT 1 cut(s) 624
Bme1390I CCNGG 3 cut(s) 12, 18, 875
Bme18I GGWCC 2 cut(s) 14, 98
BmgBI CACGTC 1 cut(s) 162
BmgT120I GGNCC 3 cut(s) 14, 98, 151
BmiI GGNNCC 4 cut(s) 99, 100, 497, 805
BmrFI CCNGG 3 cut(s) 12, 18, 875
BmsI GCATC 2 cut(s) 913, 928
Bpu14I TTCGAA 1 cut(s) 215
BsaHI GRCGYC 1 cut(s) 804
BsaI GGTCTC 1 cut(s) 685
BsaJI CCNNGG 5 cut(s) 60, 79, 130, 265, 439
BsaWI WCCGGW 1 cut(s) 395
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse1I ACTGG 2 cut(s) 124, 465
Bse3DI GCAATG 1 cut(s) 750
BseAI TCCGGA 1 cut(s) 395
BseBI CCWGG 3 cut(s) 12, 18, 875
BseDI CCNNGG 5 cut(s) 60, 79, 130, 265, 439
BseGI GGATG 1 cut(s) 886
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMI GCAATG 1 cut(s) 750
BseMII CTCAG 1 cut(s) 504
BseNI ACTGG 2 cut(s) 124, 465
BseRI GAGGAG 1 cut(s) 789
BseSI GKGCMC 1 cut(s) 68
BseXI GCAGC 3 cut(s) 13, 17, 248
Bsh1236I CGCG 2 cut(s) 34, 689
BshFI GGCC 5 cut(s) 123, 152, 191, 231, 251
BshNI GGYRCC 1 cut(s) 803
BsiSI CCGG 1 cut(s) 396
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 685
BsmFI GGGAC 1 cut(s) 84
BsmI GAATGC 2 cut(s) 347, 698
BsnI GGCC 5 cut(s) 123, 152, 191, 231, 251
Bso31I GGTCTC 1 cut(s) 685
Bsp119I TTCGAA 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 68
Bsp13I TCCGGA 1 cut(s) 395
Bsp143I GATC 3 cut(s) 156, 495, 574
Bsp19I CCATGG 1 cut(s) 130
Bsp68I TCGCGA 1 cut(s) 34
BspACI CCGC 3 cut(s) 232, 554, 687
BspANI GGCC 5 cut(s) 123, 152, 191, 231, 251
BspCNI CTCAG 1 cut(s) 503
BspEI TCCGGA 1 cut(s) 395
BspFNI CGCG 2 cut(s) 34, 689
BspLI GGNNCC 4 cut(s) 99, 100, 497, 805
BspMAI CTGCAG 1 cut(s) 634
BspMI ACCTGC 1 cut(s) 703
BspPI GGATC 2 cut(s) 490, 503
BspQI GCTCTTC 1 cut(s) 200
BspT104I TTCGAA 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 803
BspTNI GGTCTC 1 cut(s) 685
BsrBI CCGCTC 1 cut(s) 234
BsrDI GCAATG 1 cut(s) 750
BsrI ACTGG 2 cut(s) 124, 465
BssECI CCNNGG 5 cut(s) 60, 79, 130, 265, 439
BssMI GATC 3 cut(s) 156, 495, 574
BssNI GRCGYC 1 cut(s) 804
BssT1I CCWWGG 5 cut(s) 60, 79, 130, 265, 439
Bst2UI CCWGG 3 cut(s) 12, 18, 875
Bst6I CTCTTC 1 cut(s) 200
BstACI GRCGYC 1 cut(s) 804
BstAPI GCANNNNNTGC 1 cut(s) 749
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 4 cut(s) 116, 280, 422, 426
BstDEI CTNAG 1 cut(s) 490
BstDSI CCRYGG 1 cut(s) 130
BstF5I GGATG 1 cut(s) 886
BstFNI CGCG 2 cut(s) 34, 689
BstH2I RGCGCY 1 cut(s) 807
BstHHI GCGC 1 cut(s) 806
BstKTI GATC 3 cut(s) 159, 498, 577
BstMAI GTCTC 1 cut(s) 685
BstMBI GATC 3 cut(s) 156, 495, 574
BstMWI GCNNNNNNNGC 5 cut(s) 40, 120, 267, 695, 749
BstNI CCWGG 3 cut(s) 12, 18, 875
BstSCI CCNGG 3 cut(s) 10, 16, 873
BstSFI CTRYAG 2 cut(s) 109, 630
BstSLI GKGCMC 1 cut(s) 68
BstUI CGCG 2 cut(s) 34, 689
BstV1I GCAGC 3 cut(s) 13, 17, 248
BstX2I RGATCY 1 cut(s) 495
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 1 cut(s) 495
BsuRI GGCC 5 cut(s) 123, 152, 191, 231, 251
BtgI CCRYGG 1 cut(s) 130
BtrI CACGTC 1 cut(s) 162
BtsCI GGATG 1 cut(s) 886
BtsIMutI CAGTG 2 cut(s) 131, 570
BtuMI TCGCGA 1 cut(s) 34
BveI ACCTGC 1 cut(s) 703
Cac8I GCNNGC 4 cut(s) 116, 280, 422, 426
CfoI GCGC 1 cut(s) 806
Cfr13I GGNCC 3 cut(s) 14, 98, 151
CseI GACGC 1 cut(s) 18
Csp6I GTAC 3 cut(s) 623, 658, 737
CviAII CATG 6 cut(s) 131, 227, 336, 366, 655, 824
CviQI GTAC 3 cut(s) 623, 658, 737
DdeI CTNAG 1 cut(s) 490
DinI GGCGCC 1 cut(s) 805
DpnI GATC 3 cut(s) 158, 497, 576
DpnII GATC 3 cut(s) 156, 495, 574
EaeI YGGCCR 2 cut(s) 121, 229
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
Eco130I CCWWGG 5 cut(s) 60, 79, 130, 265, 439
Eco31I GGTCTC 1 cut(s) 685
Eco47I GGWCC 2 cut(s) 14, 98
Eco57I CTGAAG 2 cut(s) 289, 542
EcoO109I RGGNCCY 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 549
EcoRII CCWGG 3 cut(s) 10, 16, 873
EcoT14I CCWWGG 5 cut(s) 60, 79, 130, 265, 439
EgeI GGCGCC 1 cut(s) 805
EheI GGCGCC 1 cut(s) 805
ErhI CCWWGG 5 cut(s) 60, 79, 130, 265, 439
FaeI CATG 6 cut(s) 134, 230, 339, 369, 658, 827
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FaqI GGGAC 1 cut(s) 84
FatI CATG 6 cut(s) 130, 226, 335, 365, 654, 823
FauI CCCGC 1 cut(s) 694
FbaI TGATCA 2 cut(s) 156, 574
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 4 cut(s) 6, 27, 232, 262
FokI GGATG 1 cut(s) 893
Fsp4HI GCNGC 4 cut(s) 6, 27, 232, 262
FspBI CTAG 3 cut(s) 80, 620, 740
GlaI GCGC 1 cut(s) 805
GluI GCNGC 4 cut(s) 6, 27, 232, 262
HaeII RGCGCY 1 cut(s) 807
HaeIII GGCC 5 cut(s) 123, 152, 191, 231, 251
HapII CCGG 1 cut(s) 396
HgaI GACGC 1 cut(s) 18
HhaI GCGC 1 cut(s) 806
Hin1I GRCGYC 1 cut(s) 804
Hin1II CATG 6 cut(s) 134, 230, 339, 369, 658, 827
Hin6I GCGC 1 cut(s) 804
HinP1I GCGC 1 cut(s) 804
HincII GTYRAC 2 cut(s) 471, 565
HindII GTYRAC 2 cut(s) 471, 565
HindIII AAGCTT 1 cut(s) 418
HinfI GANTC 1 cut(s) 105
HpaII CCGG 1 cut(s) 396
Hpy166II GTNNAC 5 cut(s) 387, 458, 471, 565, 758
Hpy188I TCNGA 4 cut(s) 522, 588, 729, 913
Hpy188III TCNNGA 2 cut(s) 33, 396
Hpy8I GTNNAC 5 cut(s) 387, 458, 471, 565, 758
Hpy99I CGWCG 2 cut(s) 34, 569
HpyAV CCTTC 3 cut(s) 517, 838, 845
HpyCH4IV ACGT 1 cut(s) 161
HpyCH4V TGCA 4 cut(s) 345, 452, 632, 698
HpyF10VI GCNNNNNNNGC 5 cut(s) 40, 120, 267, 695, 749
HpyF3I CTNAG 1 cut(s) 490
HpySE526I ACGT 1 cut(s) 161
Hsp92I GRCGYC 1 cut(s) 804
Hsp92II CATG 6 cut(s) 134, 230, 339, 369, 658, 827
HspAI GCGC 1 cut(s) 804
KasI GGCGCC 1 cut(s) 803
KflI GGGWCCC 1 cut(s) 98
Kpn2I TCCGGA 1 cut(s) 395
Ksp22I TGATCA 2 cut(s) 156, 574
Kzo9I GATC 3 cut(s) 156, 495, 574
LguI GCTCTTC 1 cut(s) 200
LmnI GCTCC 2 cut(s) 13, 776
Lsp1109I GCAGC 3 cut(s) 13, 17, 248
LweI GCATC 2 cut(s) 913, 928
MaeI CTAG 3 cut(s) 80, 620, 740
MaeII ACGT 1 cut(s) 161
MaeIII GTNAC 2 cut(s) 818, 850
MalI GATC 3 cut(s) 158, 497, 576
MbiI CCGCTC 1 cut(s) 234
MboI GATC 3 cut(s) 156, 495, 574
MboII GAAGA 3 cut(s) 217, 295, 862
MflI RGATCY 1 cut(s) 495
MhlI GDGCHC 1 cut(s) 68
MluCI AATT 8 cut(s) 48, 70, 145, 316, 445, 528, 549, 706
Mly113I GGCGCC 1 cut(s) 804
MlyI GAGTC 1 cut(s) 99
MnlI CCTC 6 cut(s) 48, 175, 509, 767, 849, 871
MroI TCCGGA 1 cut(s) 395
MslI CAYNNNNRTG 1 cut(s) 340
MspA1I CMGCKG 2 cut(s) 114, 556
MspI CCGG 1 cut(s) 396
MspR9I CCNGG 3 cut(s) 12, 18, 875
Mva1269I GAATGC 2 cut(s) 347, 698
MvaI CCWGG 3 cut(s) 12, 18, 875
MvnI CGCG 2 cut(s) 34, 689
MwoI GCNNNNNNNGC 5 cut(s) 40, 120, 267, 695, 749
NarI GGCGCC 1 cut(s) 804
NcoI CCATGG 1 cut(s) 130
NdeII GATC 3 cut(s) 156, 495, 574
NlaIII CATG 6 cut(s) 134, 230, 339, 369, 658, 827
NlaIV GGNNCC 4 cut(s) 99, 100, 497, 805
NmeAIII GCCGAG 1 cut(s) 99
NruI TCGCGA 1 cut(s) 34
NspV TTCGAA 1 cut(s) 215
PciSI GCTCTTC 1 cut(s) 200
PctI GAATGC 2 cut(s) 347, 698
PfoI TCCNGGA 2 cut(s) 16, 873
PkrI GCNGC 4 cut(s) 7, 28, 233, 263
PleI GAGTC 1 cut(s) 99
PluTI GGCGCC 1 cut(s) 807
PpsI GAGTC 1 cut(s) 99
PpuMI RGGWCCY 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 98
Psp6I CCWGG 3 cut(s) 10, 16, 873
PspGI CCWGG 3 cut(s) 10, 16, 873
PspN4I GGNNCC 4 cut(s) 99, 100, 497, 805
PspPI GGNCC 3 cut(s) 14, 98, 151
PspPPI RGGWCCY 1 cut(s) 98
PstI CTGCAG 1 cut(s) 634
PsuI RGATCY 1 cut(s) 495
PvuII CAGCTG 1 cut(s) 114
RruI TCGCGA 1 cut(s) 34
RsaI GTAC 3 cut(s) 624, 659, 738
RsaNI GTAC 3 cut(s) 623, 658, 737
RseI CAYNNNNRTG 1 cut(s) 340
SalI GTCGAC 1 cut(s) 563
SapI GCTCTTC 1 cut(s) 200
SatI GCNGC 4 cut(s) 6, 27, 232, 262
Sau3AI GATC 3 cut(s) 156, 495, 574
Sau96I GGNCC 3 cut(s) 14, 98, 151
ScaI AGTACT 1 cut(s) 624
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 3 cut(s) 12, 18, 875
SduI GDGCHC 1 cut(s) 68
SfaNI GCATC 2 cut(s) 913, 928
SfcI CTRYAG 2 cut(s) 109, 630
SfoI GGCGCC 1 cut(s) 805
SfuI TTCGAA 1 cut(s) 215
SinI GGWCC 2 cut(s) 14, 98
SmiMI CAYNNNNRTG 1 cut(s) 340
Sse9I AATT 8 cut(s) 48, 70, 145, 316, 445, 528, 549, 706
SsiI CCGC 3 cut(s) 232, 554, 687
SspDI GGCGCC 1 cut(s) 803
SspMI CTAG 3 cut(s) 80, 620, 740
StyD4I CCNGG 3 cut(s) 10, 16, 873
StyI CCWWGG 5 cut(s) 60, 79, 130, 265, 439
TaiI ACGT 1 cut(s) 164
TaqI TCGA 2 cut(s) 215, 564
TasI AATT 8 cut(s) 48, 70, 145, 316, 445, 528, 549, 706
TatI WGTACW 2 cut(s) 622, 736
TauI GCSGC 1 cut(s) 234
TscAI CASTG 2 cut(s) 131, 577
TseI GCWGC 3 cut(s) 5, 26, 261
TspDTI ATGAA 5 cut(s) 605, 634, 643, 746, 932
TspRI CASTG 2 cut(s) 131, 577
VpaK11BI GGWCC 2 cut(s) 14, 98
XapI RAATTY 3 cut(s) 70, 316, 549
XmaJI CCTAGG 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 564
XspI CTAG 3 cut(s) 80, 620, 740
ZrmI AGTACT 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.