Rroxscaffold_6G00429890

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004430
Physical Location & Seq
Reverse (-)
50058982 .. 50061734
2753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00429890.1

Sequence Viewer

Length: 930 bp
ATGGCAGCTCCAGGTCCAGGAGAAGCTGTGTCGCGACCTCTGCTCCCAATTCCAATTCCACAGGGCACAAATTCAACCCTAGGCGAAGCGATTTCGGGTCCCAGATTCTACAGCTGGCTCGGCCAGTGCCATGGGTTCTGGCACAATTTGGCCCTGATCACGTCCTCGCTTCTCTTTGCTCTATACTTGGCCTATCAATCAAGGAAAAGCTTTTCGAAGCTCTATCATGGCCGCTCGTATATTATGATGGCCTACTATGGGTGCCTTTGGCTTGTTAGCTTGCTCAATCTCGCTTGGTGCTTTCTTCAGGCATGGGAATGCACACCTGATAAAGAAATGGCATGGAATGTCTTATCTTTGTTCACAACTTCCGGAATGTTATTTTTGGAAGTAAGCTTGCTGGCATTTTTACTCCAAGGGAATTATGCAAGTGGACTGGAAGCGTTGACACGAACTTTTGTTATCTCAGGGATCCTCGTTGGTTTGGATTTACTTCTGAAGGCAATTTATATGTTTGGATTTGGAATTCCACTGTTTGTCGACGACACTGATCATACACATCAGATGAAGTGGAACTTGTGGTCTGTCCATAGGCTAGTACTAACTGCAGTTTATGGGTTCATATTGTTCATGTACCATTCCAAGTGGAGAGAAAGGCTACCCGCGAGACCTGCATTCTACAATTATACAGCTATCATGTTTCTGATGAATGTACTAGCAATGTTTGCTTGTGGACTTACAGGAAATGGAGGAGCTGGATTTGGGTTTTGGTTATATGGCGCCACTATTGTCTGTTACCATGCCTTCTATCTTCCTCTTTTATATGTAACATTTCTGGCAGACTTCTTCCAGGAGGATGATTTACATTTGGAGAATGTATATTATTCAGAGATGAAAGATGCTGGTTTCTTTGATGCTGATTGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

309

Amino Acids

35.16

Weight (kDa)

5.81

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_40 PF10160 42 - 296 1.5e-119 Predicted membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 679
AccB1I GGYRCC 2 cut(s) 261, 779
AccBSI CCGCTC 1 cut(s) 234
AccI GTMKAC 1 cut(s) 540
AccII CGCG 2 cut(s) 34, 665
AccIII TCCGGA 1 cut(s) 371
AciI CCGC 2 cut(s) 232, 663
AclWI GGATC 2 cut(s) 466, 479
AcoI YGGCCR 2 cut(s) 121, 229
AcsI RAATTY 2 cut(s) 70, 525
AcuI CTGAAG 2 cut(s) 290, 518
AcyI GRCGYC 1 cut(s) 780
AfaI GTAC 3 cut(s) 600, 635, 714
AfiI CCNNNNNNNGG 2 cut(s) 17, 258
AgsI TTSAA 1 cut(s) 75
AjiI CACGTC 1 cut(s) 162
AjnI CCWGG 3 cut(s) 10, 16, 849
AluBI AGCT 9 cut(s) 8, 26, 114, 210, 220, 279, 396, 692, 755
AluI AGCT 9 cut(s) 8, 26, 114, 210, 220, 279, 396, 692, 755
Alw26I GTCTC 1 cut(s) 661
AlwI GGATC 2 cut(s) 466, 479
Aor13HI TCCGGA 1 cut(s) 371
AoxI GGCC 5 cut(s) 121, 150, 189, 229, 249
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 70, 525
AspA2I CCTAGG 1 cut(s) 79
AspLEI GCGC 1 cut(s) 782
AspS9I GGNCC 3 cut(s) 14, 98, 151
AsuII TTCGAA 1 cut(s) 215
AvaII GGWCC 2 cut(s) 14, 98
AvrII CCTAGG 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 68
BamHI GGATCC 1 cut(s) 471
BanI GGYRCC 2 cut(s) 261, 779
BbvI GCAGC 1 cut(s) 17
BccI CCATC 1 cut(s) 241
BciT130I CCWGG 3 cut(s) 12, 18, 851
BclI TGATCA 2 cut(s) 156, 550
BcoDI GTCTC 1 cut(s) 661
BfaI CTAG 3 cut(s) 80, 596, 716
BfmI CTRYAG 2 cut(s) 109, 606
BfoI RGCGCY 1 cut(s) 783
BfuAI ACCTGC 1 cut(s) 679
BisI GCNGC 2 cut(s) 6, 232
BlnI CCTAGG 1 cut(s) 79
BlsI GCNGC 2 cut(s) 7, 233
BmcAI AGTACT 1 cut(s) 600
Bme1390I CCNGG 3 cut(s) 12, 18, 851
Bme18I GGWCC 2 cut(s) 14, 98
BmgBI CACGTC 1 cut(s) 162
BmgT120I GGNCC 3 cut(s) 14, 98, 151
BmiI GGNNCC 5 cut(s) 99, 100, 263, 473, 781
BmrFI CCNGG 3 cut(s) 12, 18, 851
BmsI GCATC 2 cut(s) 889, 904
Bpu14I TTCGAA 1 cut(s) 215
BsaHI GRCGYC 1 cut(s) 780
BsaI GGTCTC 1 cut(s) 661
BsaJI CCNNGG 3 cut(s) 79, 130, 415
BsaWI WCCGGW 1 cut(s) 371
BsaXI ACNNNNNCTCC 4 cut(s) 12, 27, 42, 57
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 258
Bse1I ACTGG 2 cut(s) 124, 441
Bse3DI GCAATG 1 cut(s) 726
BseAI TCCGGA 1 cut(s) 371
BseBI CCWGG 3 cut(s) 12, 18, 851
BseDI CCNNGG 3 cut(s) 79, 130, 415
BseGI GGATG 1 cut(s) 862
BseLI CCNNNNNNNGG 2 cut(s) 17, 258
BseMI GCAATG 1 cut(s) 726
BseMII CTCAG 1 cut(s) 480
BseNI ACTGG 2 cut(s) 124, 441
BseRI GAGGAG 1 cut(s) 765
BseSI GKGCMC 1 cut(s) 68
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 2 cut(s) 34, 665
BshFI GGCC 5 cut(s) 123, 152, 191, 231, 251
BshNI GGYRCC 2 cut(s) 261, 779
BsiSI CCGG 1 cut(s) 372
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 2 cut(s) 17, 258
BsmAI GTCTC 1 cut(s) 661
BsmFI GGGAC 1 cut(s) 84
BsmI GAATGC 2 cut(s) 323, 674
BsnI GGCC 5 cut(s) 123, 152, 191, 231, 251
Bso31I GGTCTC 1 cut(s) 661
Bsp119I TTCGAA 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 68
Bsp13I TCCGGA 1 cut(s) 371
Bsp143I GATC 3 cut(s) 156, 471, 550
Bsp19I CCATGG 1 cut(s) 130
Bsp68I TCGCGA 1 cut(s) 34
BspACI CCGC 2 cut(s) 232, 663
BspANI GGCC 5 cut(s) 123, 152, 191, 231, 251
BspCNI CTCAG 1 cut(s) 479
BspEI TCCGGA 1 cut(s) 371
BspFNI CGCG 2 cut(s) 34, 665
BspLI GGNNCC 5 cut(s) 99, 100, 263, 473, 781
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 1 cut(s) 679
BspPI GGATC 2 cut(s) 466, 479
BspT104I TTCGAA 1 cut(s) 215
BspT107I GGYRCC 2 cut(s) 261, 779
BspTNI GGTCTC 1 cut(s) 661
BsrBI CCGCTC 1 cut(s) 234
BsrDI GCAATG 1 cut(s) 726
BsrI ACTGG 2 cut(s) 124, 441
BssECI CCNNGG 3 cut(s) 79, 130, 415
BssMI GATC 3 cut(s) 156, 471, 550
BssNI GRCGYC 1 cut(s) 780
BssT1I CCWWGG 3 cut(s) 79, 130, 415
Bst2UI CCWGG 3 cut(s) 12, 18, 851
Bst4CI ACNGT 1 cut(s) 534
BstACI GRCGYC 1 cut(s) 780
BstAPI GCANNNNNTGC 1 cut(s) 725
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 4 cut(s) 116, 281, 398, 402
BstDEI CTNAG 1 cut(s) 466
BstDSI CCRYGG 1 cut(s) 130
BstF5I GGATG 1 cut(s) 862
BstFNI CGCG 2 cut(s) 34, 665
BstH2I RGCGCY 1 cut(s) 783
BstHHI GCGC 1 cut(s) 782
BstKTI GATC 3 cut(s) 159, 474, 553
BstMAI GTCTC 1 cut(s) 661
BstMBI GATC 3 cut(s) 156, 471, 550
BstMWI GCNNNNNNNGC 4 cut(s) 40, 120, 671, 725
BstNI CCWGG 3 cut(s) 12, 18, 851
BstSCI CCNGG 3 cut(s) 10, 16, 849
BstSFI CTRYAG 2 cut(s) 109, 606
BstSLI GKGCMC 1 cut(s) 68
BstUI CGCG 2 cut(s) 34, 665
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 471
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 1 cut(s) 471
BsuRI GGCC 5 cut(s) 123, 152, 191, 231, 251
BtgI CCRYGG 1 cut(s) 130
BtrI CACGTC 1 cut(s) 162
BtsCI GGATG 1 cut(s) 862
BtsIMutI CAGTG 3 cut(s) 131, 530, 546
BtuMI TCGCGA 1 cut(s) 34
BveI ACCTGC 1 cut(s) 679
Cac8I GCNNGC 4 cut(s) 116, 281, 398, 402
CfoI GCGC 1 cut(s) 782
Cfr13I GGNCC 3 cut(s) 14, 98, 151
Csp6I GTAC 3 cut(s) 599, 634, 713
CviAII CATG 7 cut(s) 131, 227, 312, 342, 631, 697, 800
CviQI GTAC 3 cut(s) 599, 634, 713
DdeI CTNAG 1 cut(s) 466
DinI GGCGCC 1 cut(s) 781
DpnI GATC 3 cut(s) 158, 473, 552
DpnII GATC 3 cut(s) 156, 471, 550
EaeI YGGCCR 2 cut(s) 121, 229
Eco130I CCWWGG 3 cut(s) 79, 130, 415
Eco31I GGTCTC 1 cut(s) 661
Eco47I GGWCC 2 cut(s) 14, 98
Eco57I CTGAAG 2 cut(s) 290, 518
EcoO109I RGGNCCY 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 525
EcoRII CCWGG 3 cut(s) 10, 16, 849
EcoT14I CCWWGG 3 cut(s) 79, 130, 415
EgeI GGCGCC 1 cut(s) 781
EheI GGCGCC 1 cut(s) 781
ErhI CCWWGG 3 cut(s) 79, 130, 415
FaeI CATG 7 cut(s) 134, 230, 315, 345, 634, 700, 803
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 1 cut(s) 84
FatI CATG 7 cut(s) 130, 226, 311, 341, 630, 696, 799
FauI CCCGC 1 cut(s) 670
FbaI TGATCA 2 cut(s) 156, 550
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 2 cut(s) 6, 232
FokI GGATG 1 cut(s) 869
Fsp4HI GCNGC 2 cut(s) 6, 232
FspBI CTAG 3 cut(s) 80, 596, 716
GlaI GCGC 1 cut(s) 781
GluI GCNGC 2 cut(s) 6, 232
HaeII RGCGCY 1 cut(s) 783
HaeIII GGCC 5 cut(s) 123, 152, 191, 231, 251
HapII CCGG 1 cut(s) 372
HhaI GCGC 1 cut(s) 782
Hin1I GRCGYC 1 cut(s) 780
Hin1II CATG 7 cut(s) 134, 230, 315, 345, 634, 700, 803
Hin6I GCGC 1 cut(s) 780
HinP1I GCGC 1 cut(s) 780
HincII GTYRAC 2 cut(s) 447, 541
HindII GTYRAC 2 cut(s) 447, 541
HindIII AAGCTT 2 cut(s) 208, 394
HinfI GANTC 1 cut(s) 105
HpaII CCGG 1 cut(s) 372
Hpy166II GTNNAC 5 cut(s) 363, 434, 447, 541, 734
Hpy188I TCNGA 4 cut(s) 498, 564, 705, 889
Hpy188III TCNNGA 2 cut(s) 33, 372
Hpy8I GTNNAC 5 cut(s) 363, 434, 447, 541, 734
Hpy99I CGWCG 1 cut(s) 545
HpyAV CCTTC 2 cut(s) 493, 814
HpyCH4III ACNGT 1 cut(s) 534
HpyCH4IV ACGT 1 cut(s) 161
HpyCH4V TGCA 4 cut(s) 321, 428, 608, 674
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 120, 671, 725
HpyF3I CTNAG 1 cut(s) 466
HpySE526I ACGT 1 cut(s) 161
Hsp92I GRCGYC 1 cut(s) 780
Hsp92II CATG 7 cut(s) 134, 230, 315, 345, 634, 700, 803
HspAI GCGC 1 cut(s) 780
KasI GGCGCC 1 cut(s) 779
KflI GGGWCCC 1 cut(s) 98
Kpn2I TCCGGA 1 cut(s) 371
Ksp22I TGATCA 2 cut(s) 156, 550
Kzo9I GATC 3 cut(s) 156, 471, 550
LmnI GCTCC 3 cut(s) 13, 48, 752
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 2 cut(s) 889, 904
MaeI CTAG 3 cut(s) 80, 596, 716
MaeII ACGT 1 cut(s) 161
MaeIII GTNAC 2 cut(s) 794, 826
MalI GATC 3 cut(s) 158, 473, 552
MbiI CCGCTC 1 cut(s) 234
MboI GATC 3 cut(s) 156, 471, 550
MboII GAAGA 3 cut(s) 296, 803, 838
MflI RGATCY 1 cut(s) 471
MhlI GDGCHC 1 cut(s) 68
MluCI AATT 8 cut(s) 48, 54, 70, 145, 421, 504, 525, 682
Mly113I GGCGCC 1 cut(s) 780
MnlI CCTC 6 cut(s) 48, 175, 485, 743, 825, 847
MroI TCCGGA 1 cut(s) 371
MslI CAYNNNNRTG 1 cut(s) 316
MspA1I CMGCKG 1 cut(s) 114
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 3 cut(s) 12, 18, 851
Mva1269I GAATGC 2 cut(s) 323, 674
MvaI CCWGG 3 cut(s) 12, 18, 851
MvnI CGCG 2 cut(s) 34, 665
MwoI GCNNNNNNNGC 4 cut(s) 40, 120, 671, 725
NarI GGCGCC 1 cut(s) 780
NcoI CCATGG 1 cut(s) 130
NdeII GATC 3 cut(s) 156, 471, 550
NlaIII CATG 7 cut(s) 134, 230, 315, 345, 634, 700, 803
NlaIV GGNNCC 5 cut(s) 99, 100, 263, 473, 781
NmeAIII GCCGAG 1 cut(s) 99
NruI TCGCGA 1 cut(s) 34
NspV TTCGAA 1 cut(s) 215
PctI GAATGC 2 cut(s) 323, 674
PfeI GAWTC 1 cut(s) 105
PfoI TCCNGGA 2 cut(s) 16, 849
PkrI GCNGC 2 cut(s) 7, 233
PluTI GGCGCC 1 cut(s) 783
PpuMI RGGWCCY 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 98
Psp6I CCWGG 3 cut(s) 10, 16, 849
PspGI CCWGG 3 cut(s) 10, 16, 849
PspN4I GGNNCC 5 cut(s) 99, 100, 263, 473, 781
PspPI GGNCC 3 cut(s) 14, 98, 151
PspPPI RGGWCCY 1 cut(s) 98
PstI CTGCAG 1 cut(s) 610
PsuI RGATCY 1 cut(s) 471
PvuII CAGCTG 1 cut(s) 114
RruI TCGCGA 1 cut(s) 34
RsaI GTAC 3 cut(s) 600, 635, 714
RsaNI GTAC 3 cut(s) 599, 634, 713
RseI CAYNNNNRTG 1 cut(s) 316
SalI GTCGAC 1 cut(s) 539
SatI GCNGC 2 cut(s) 6, 232
Sau3AI GATC 3 cut(s) 156, 471, 550
Sau96I GGNCC 3 cut(s) 14, 98, 151
ScaI AGTACT 1 cut(s) 600
ScrFI CCNGG 3 cut(s) 12, 18, 851
SduI GDGCHC 1 cut(s) 68
SfaNI GCATC 2 cut(s) 889, 904
SfcI CTRYAG 2 cut(s) 109, 606
SfoI GGCGCC 1 cut(s) 781
SfuI TTCGAA 1 cut(s) 215
SinI GGWCC 2 cut(s) 14, 98
SmiMI CAYNNNNRTG 1 cut(s) 316
Sse9I AATT 8 cut(s) 48, 54, 70, 145, 421, 504, 525, 682
SsiI CCGC 2 cut(s) 232, 663
SspDI GGCGCC 1 cut(s) 779
SspMI CTAG 3 cut(s) 80, 596, 716
StyD4I CCNGG 3 cut(s) 10, 16, 849
StyI CCWWGG 3 cut(s) 79, 130, 415
TaaI ACNGT 1 cut(s) 534
TaiI ACGT 1 cut(s) 164
TaqI TCGA 2 cut(s) 215, 540
TasI AATT 8 cut(s) 48, 54, 70, 145, 421, 504, 525, 682
TatI WGTACW 2 cut(s) 598, 712
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 1 cut(s) 105
TscAI CASTG 3 cut(s) 131, 537, 553
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 5 cut(s) 581, 610, 619, 722, 908
TspRI CASTG 3 cut(s) 131, 537, 553
VpaK11BI GGWCC 2 cut(s) 14, 98
XapI RAATTY 2 cut(s) 70, 525
XmaJI CCTAGG 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 540
XspI CTAG 3 cut(s) 80, 596, 716
ZrmI AGTACT 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.