RchiOBHm_Chr7g0182971

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004430
Physical Location & Seq
Forward (+)
4018832 .. 4021411
2580 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16321

Sequence Viewer

Length: 906 bp
ATGGCAACAGATCTCCTACAACACCTGAACGCCTCGGTCAACCCGCCGATCCCAAGCCTTCCCGCCACCGTCGACGGCGAGGATCGGAGGTTGTCCAGCTCAAACTGCCATGGCCTCTGGTACAGCGCCGTTCTCGTCGTCCCTTCCGTCATTTTCTTGGTCTACTTGGCCTTCCACGCCAAGAAGAACCTCAAGAAGCTCTCAAATGGCAGCTCTTATATCATGATTTCCTATTATGCCCTCGTCTGGGTCGCCGCCCTCCTCAACCTCGCCTGGTGCGCTCTTCAGGGTTGGCAATGCTCTGGTGGAAAGGCGGTGGCTTGGAATCTGTTGACTTTGTTCACAGCTTCTGCAATGATGTGTTTGGAGATTAGCTTGGTGGCTTTTTTGCTCCAGGACAATTACTCGACTGGAGTTGAAGCTCTAGCTCAGACTTTCCTTTTCTCAGGGATCGTTGTGAGCGTGGATACACTGCTCAAGGCAATCTATGTATTTGGATTTGGGGTCCCTCTCTTCATGGTTGGTGTTGGAGGTACGACTGGAGTGAAATGGGGCCTATGGTTCTTCCACAAATCATTGCTTACTGCAGTCTATGGCTTCATATTGTTTGTGCATTTCTCCAAATGGAGAGAAAAGCTTCCGCCCAGACCAGCCTTCTACAACTACATTGTCGTGATGTTTGTATTTGGTGCTGTGACAATGTTTGCTTGTGGGCTTGCTGGAATCGGGGCTGGTTTTGGCATTTGGTTGTACAATTTGACAGAAATATGTTACCACGCTCTCTATCTTCCTTTTCTATATGTAACCTTCCTGGCAGATTTTTTCCAGGAAGAACAATTTCTTCTGGATAATGCATATTACTCTGAGATGAAGGATGCAGGGTTCTTCGATGCTGATTGGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

301

Amino Acids

33.55

Weight (kDa)

5.83

Isoelectric Point (pI)

32.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_40 PF10160 35 - 288 1.6e-107 Predicted membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 72, 162
AciI CCGC 5 cut(s) 44, 63, 255, 314, 641
AclWI GGATC 3 cut(s) 43, 90, 458
AcuI CTGAAG 1 cut(s) 269
AfaI GTAC 3 cut(s) 122, 535, 752
AfiI CCNNNNNNNGG 2 cut(s) 246, 247
AgsI TTSAA 1 cut(s) 419
AjnI CCWGG 4 cut(s) 272, 393, 810, 825
AloI GAACNNNNNNTCC 2 cut(s) 866, 898
AluBI AGCT 8 cut(s) 99, 199, 213, 347, 375, 422, 428, 637
AluI AGCT 8 cut(s) 99, 199, 213, 347, 375, 422, 428, 637
AlwI GGATC 3 cut(s) 43, 90, 458
AlwNI CAGNNNCTG 1 cut(s) 350
AoxI GGCC 3 cut(s) 112, 168, 553
ApeKI GCWGC 1 cut(s) 210
Asp700I GAANNNNTTC 2 cut(s) 636, 837
AspLEI GCGC 2 cut(s) 128, 281
AspS9I GGNCC 2 cut(s) 505, 553
AvaII GGWCC 1 cut(s) 505
BbvI GCAGC 1 cut(s) 222
BceAI ACGGC 2 cut(s) 91, 113
BciT130I CCWGG 4 cut(s) 274, 395, 812, 827
BciVI GTATCC 1 cut(s) 460
BfaI CTAG 1 cut(s) 425
BfmI CTRYAG 1 cut(s) 585
BfoI RGCGCY 1 cut(s) 129
BfuI GTATCC 1 cut(s) 460
BglII AGATCT 1 cut(s) 10
BisI GCNGC 2 cut(s) 211, 255
BlsI GCNGC 2 cut(s) 212, 256
Bme1390I CCNGG 4 cut(s) 274, 395, 812, 827
Bme18I GGWCC 1 cut(s) 505
BmgT120I GGNCC 2 cut(s) 505, 553
BmiI GGNNCC 3 cut(s) 506, 507, 554
BmrFI CCNGG 4 cut(s) 274, 395, 812, 827
BmsI GCATC 2 cut(s) 865, 880
BpmI CTGGAG 3 cut(s) 377, 432, 561
BpuEI CTTGAG 2 cut(s) 176, 461
BsaJI CCNNGG 2 cut(s) 33, 109
Bsc4I CCNNNNNNNGG 2 cut(s) 246, 247
Bse1I ACTGG 2 cut(s) 415, 544
Bse3DI GCAATG 3 cut(s) 302, 360, 575
BseBI CCWGG 4 cut(s) 274, 395, 812, 827
BseDI CCNNGG 2 cut(s) 33, 109
BseGI GGATG 1 cut(s) 880
BseLI CCNNNNNNNGG 2 cut(s) 246, 247
BseMI GCAATG 3 cut(s) 302, 360, 575
BseMII CTCAG 3 cut(s) 443, 459, 855
BseNI ACTGG 2 cut(s) 415, 544
BseRI GAGGAG 1 cut(s) 251
BseXI GCAGC 1 cut(s) 222
BshFI GGCC 3 cut(s) 114, 170, 555
BslFI GGGAC 2 cut(s) 125, 491
BslI CCNNNNNNNGG 2 cut(s) 246, 247
BsmFI GGGAC 2 cut(s) 125, 491
BsnI GGCC 3 cut(s) 114, 170, 555
Bsp1407I TGTACA 1 cut(s) 750
Bsp143I GATC 4 cut(s) 10, 48, 82, 450
Bsp19I CCATGG 1 cut(s) 109
BspACI CCGC 5 cut(s) 44, 63, 255, 314, 641
BspANI GGCC 3 cut(s) 114, 170, 555
BspCNI CTCAG 3 cut(s) 442, 458, 856
BspHI TCATGA 1 cut(s) 222
BspLI GGNNCC 3 cut(s) 506, 507, 554
BspMAI CTGCAG 1 cut(s) 589
BspPI GGATC 3 cut(s) 43, 90, 458
BspQI GCTCTTC 1 cut(s) 288
BsrDI GCAATG 3 cut(s) 302, 360, 575
BsrGI TGTACA 1 cut(s) 750
BsrI ACTGG 2 cut(s) 415, 544
BssECI CCNNGG 2 cut(s) 33, 109
BssMI GATC 4 cut(s) 10, 48, 82, 450
BssT1I CCWWGG 1 cut(s) 109
Bst2UI CCWGG 4 cut(s) 274, 395, 812, 827
Bst4CI ACNGT 1 cut(s) 70
Bst6I CTCTTC 2 cut(s) 288, 518
BstAUI TGTACA 1 cut(s) 750
BstC8I GCNNGC 1 cut(s) 717
BstDEI CTNAG 3 cut(s) 429, 445, 864
BstDSI CCRYGG 1 cut(s) 109
BstF5I GGATG 1 cut(s) 880
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 2 cut(s) 128, 281
BstKTI GATC 4 cut(s) 13, 51, 85, 453
BstMBI GATC 4 cut(s) 10, 48, 82, 450
BstMWI GCNNNNNNNGC 3 cut(s) 105, 176, 278
BstNI CCWGG 4 cut(s) 274, 395, 812, 827
BstSCI CCNGG 4 cut(s) 272, 393, 810, 825
BstSFI CTRYAG 1 cut(s) 585
BstV1I GCAGC 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 10
BstYI RGATCY 1 cut(s) 10
BsuI GTATCC 1 cut(s) 460
BsuRI GGCC 3 cut(s) 114, 170, 555
BtgI CCRYGG 1 cut(s) 109
BtsCI GGATG 1 cut(s) 880
BtsI GCAGTG 1 cut(s) 470
BtsIMutI CAGTG 1 cut(s) 470
Cac8I GCNNGC 1 cut(s) 717
CaiI CAGNNNCTG 1 cut(s) 350
CciI TCATGA 1 cut(s) 222
CfoI GCGC 2 cut(s) 128, 281
Cfr13I GGNCC 2 cut(s) 505, 553
Csp6I GTAC 3 cut(s) 121, 534, 751
CviAII CATG 3 cut(s) 110, 223, 517
CviQI GTAC 3 cut(s) 121, 534, 751
DdeI CTNAG 3 cut(s) 429, 445, 864
DpnI GATC 4 cut(s) 12, 50, 84, 452
DpnII GATC 4 cut(s) 10, 48, 82, 450
Eam1104I CTCTTC 2 cut(s) 288, 518
EarI CTCTTC 2 cut(s) 288, 518
EciI GGCGGA 1 cut(s) 630
Eco130I CCWWGG 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 269
EcoO109I RGGNCCY 2 cut(s) 505, 553
EcoRII CCWGG 4 cut(s) 272, 393, 810, 825
EcoT14I CCWWGG 1 cut(s) 109
EcoT22I ATGCAT 1 cut(s) 856
ErhI CCWWGG 1 cut(s) 109
FaeI CATG 3 cut(s) 113, 226, 520
FaqI GGGAC 2 cut(s) 125, 491
FatI CATG 3 cut(s) 109, 222, 516
FauI CCCGC 2 cut(s) 51, 70
FblI GTMKAC 2 cut(s) 72, 162
Fnu4HI GCNGC 2 cut(s) 211, 255
FokI GGATG 1 cut(s) 887
Fsp4HI GCNGC 2 cut(s) 211, 255
FspBI CTAG 1 cut(s) 425
GlaI GCGC 2 cut(s) 127, 280
GluI GCNGC 2 cut(s) 211, 255
GsuI CTGGAG 3 cut(s) 377, 432, 561
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 3 cut(s) 114, 170, 555
HhaI GCGC 2 cut(s) 128, 281
Hin1II CATG 3 cut(s) 113, 226, 520
Hin6I GCGC 2 cut(s) 126, 279
HinP1I GCGC 2 cut(s) 126, 279
HincII GTYRAC 3 cut(s) 40, 73, 333
HindII GTYRAC 3 cut(s) 40, 73, 333
HindIII AAGCTT 1 cut(s) 635
HinfI GANTC 2 cut(s) 325, 723
Hpy166II GTNNAC 5 cut(s) 40, 73, 163, 333, 342
Hpy188I TCNGA 3 cut(s) 87, 432, 865
Hpy188III TCNNGA 4 cut(s) 193, 223, 673, 845
Hpy8I GTNNAC 5 cut(s) 40, 73, 163, 333, 342
Hpy99I CGWCG 3 cut(s) 74, 77, 140
HpyAV CCTTC 6 cut(s) 68, 153, 181, 664, 817, 865
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4V TGCA 5 cut(s) 353, 587, 613, 854, 878
HpyF10VI GCNNNNNNNGC 3 cut(s) 105, 176, 278
HpyF3I CTNAG 3 cut(s) 429, 445, 864
Hsp92II CATG 3 cut(s) 113, 226, 520
HspAI GCGC 2 cut(s) 126, 279
KflI GGGWCCC 1 cut(s) 505
Kzo9I GATC 4 cut(s) 10, 48, 82, 450
LguI GCTCTTC 1 cut(s) 288
LmnI GCTCC 1 cut(s) 396
Lsp1109I GCAGC 1 cut(s) 222
LweI GCATC 2 cut(s) 865, 880
MaeI CTAG 1 cut(s) 425
MaeIII GTNAC 3 cut(s) 694, 770, 802
MalI GATC 4 cut(s) 12, 50, 84, 452
MboI GATC 4 cut(s) 10, 48, 82, 450
MboII GAAGA 8 cut(s) 196, 275, 505, 556, 779, 833, 842, 877
MflI RGATCY 1 cut(s) 10
MluCI AATT 3 cut(s) 400, 754, 836
MmeI TCCRAC 1 cut(s) 508
Mph1103I ATGCAT 1 cut(s) 856
MroXI GAANNNNTTC 2 cut(s) 636, 837
MslI CAYNNNNRTG 1 cut(s) 671
MspR9I CCNGG 4 cut(s) 274, 395, 812, 827
MvaI CCWGG 4 cut(s) 274, 395, 812, 827
MwoI GCNNNNNNNGC 3 cut(s) 105, 176, 278
NcoI CCATGG 1 cut(s) 109
NdeII GATC 4 cut(s) 10, 48, 82, 450
NlaIII CATG 3 cut(s) 113, 226, 520
NlaIV GGNNCC 3 cut(s) 506, 507, 554
NmuCI GTSAC 1 cut(s) 694
NsiI ATGCAT 1 cut(s) 856
PagI TCATGA 1 cut(s) 222
PciSI GCTCTTC 1 cut(s) 288
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 459
PdmI GAANNNNTTC 2 cut(s) 636, 837
PfeI GAWTC 2 cut(s) 325, 723
PfoI TCCNGGA 2 cut(s) 393, 825
PkrI GCNGC 2 cut(s) 212, 256
PpuMI RGGWCCY 1 cut(s) 505
Psp5II RGGWCCY 1 cut(s) 505
Psp6I CCWGG 4 cut(s) 272, 393, 810, 825
PspGI CCWGG 4 cut(s) 272, 393, 810, 825
PspN4I GGNNCC 3 cut(s) 506, 507, 554
PspPI GGNCC 2 cut(s) 505, 553
PspPPI RGGWCCY 1 cut(s) 505
PstI CTGCAG 1 cut(s) 589
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 1 cut(s) 10
RsaI GTAC 3 cut(s) 122, 535, 752
RsaNI GTAC 3 cut(s) 121, 534, 751
RseI CAYNNNNRTG 1 cut(s) 671
SalI GTCGAC 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 288
SatI GCNGC 2 cut(s) 211, 255
Sau3AI GATC 4 cut(s) 10, 48, 82, 450
Sau96I GGNCC 2 cut(s) 505, 553
ScrFI CCNGG 4 cut(s) 274, 395, 812, 827
SfaNI GCATC 2 cut(s) 865, 880
SfcI CTRYAG 1 cut(s) 585
SgrDI CGTCGACG 1 cut(s) 71
SinI GGWCC 1 cut(s) 505
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 2 cut(s) 191, 476
SmoI CTYRAG 2 cut(s) 191, 476
Sse9I AATT 3 cut(s) 400, 754, 836
SsiI CCGC 5 cut(s) 44, 63, 255, 314, 641
SspMI CTAG 1 cut(s) 425
StyD4I CCNGG 4 cut(s) 272, 393, 810, 825
StyI CCWWGG 1 cut(s) 109
TaaI ACNGT 1 cut(s) 70
TaqI TCGA 3 cut(s) 72, 407, 888
TaqII GACCGA 1 cut(s) 25
TasI AATT 3 cut(s) 400, 754, 836
TatI WGTACW 1 cut(s) 750
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 2 cut(s) 325, 723
TscAI CASTG 1 cut(s) 477
TseFI GTSAC 1 cut(s) 694
TseI GCWGC 1 cut(s) 210
Tsp45I GTSAC 1 cut(s) 694
TspDTI ATGAA 3 cut(s) 505, 589, 884
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 1 cut(s) 477
VpaK11BI GGWCC 1 cut(s) 505
XmiI GTMKAC 2 cut(s) 72, 162
XmnI GAANNNNTTC 2 cut(s) 636, 837
XspI CTAG 1 cut(s) 425
Zsp2I ATGCAT 1 cut(s) 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.