MD12G1231400.v1.1

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004430
Physical Location & Seq
Reverse (-)
30644486 .. 30646773
2288 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1231400.v1.1.491

Sequence Viewer

Length: 921 bp
ATGCAAGCTCCCCAAGAAGCTTCCTCGCAATCCGGACTCCCAATTCCACTCACCCAGGCCCCAAATTCAACCATAGACGACGGCGTTTCGGGCTCCGAATTCTACAGCTGGCTCGGCGAATGTCATGGGTTCTTCCACAATGTGGCTCTGATGGTGCCCTCGCTTCTCTTCGTCCTGTATTTGGCGTTTCAATCGAGAAAAAGCTTCTCGAAGCTCTCCCATGGCCGCTCTTATATCATGATTGCCTACTACGGGTGCCTCTGGCTTGTTAGCTTGCTCAATCTTGCTTGGTGCTGTTTTCAGGCATGGGAGTGCAGTCCTGGAAAAGAAGTAACATGGAATATATTATCTTTGTTCACAACCTCCGGGATGCTATTTTTGGAAGTAAGCTTGCTGGCATTTTTGCTTCAGGGGAATTATGCAAGTGGACTGGAAGCTTTGACACGGACTTTTTTTATCTCAGGGATCCTTGTTGTTTTGGATATACTTCTCAAGGCAATATATCTATTTGGACTTGGCATTCCATTGTTCATTGACAATAATGACCGTGCACATCGAACAAAGTGGGCCTTGTGGGTTGTCCACAGGCTAGTGATTACTGCAATTTACGGCTTCATATTGTTCATGTACCATTCCAAGTGGAGAGAGAGGTTACCAGCAAGACCTGCATTCTACAAGTATACCGCTATCATGTTTACGTTAAACGTACTAGTACTGTTTGCTTGCTTGCTTACTGGAAATGGGGCTGGATTTGGATTCTGGCTATATAGTGCTACTATTGTCTGTTATCACGCCTTTTATCTTCCACTTCTGTACGTAACATTTCTGGCAGACTTCTTCCAGGAGGAAGATTTAAATTTGGAGAATGTGTACTATTCAGAAATGAAAGATGCCGGTTTCTTTGACGCGGATTGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

307

Amino Acids

34.89

Weight (kDa)

5.63

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_40 PF10160 40 - 293 2e-120 Predicted membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 673
AccB1I GGYRCC 2 cut(s) 154, 255
AccB7I CCANNNNNTGG 1 cut(s) 142
AccBSI CCGCTC 1 cut(s) 228
AccI GTMKAC 1 cut(s) 680
AccII CGCG 1 cut(s) 908
AccIII TCCGGA 1 cut(s) 32
AciI CCGC 3 cut(s) 226, 684, 908
AclWI GGATC 2 cut(s) 460, 473
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 3 cut(s) 64, 98, 856
AcuI CTGAAG 1 cut(s) 392
AdeI CACNNNGTG 1 cut(s) 142
AfaI GTAC 5 cut(s) 629, 708, 714, 815, 872
AfiI CCNNNNNNNGG 3 cut(s) 142, 181, 252
AgsI TTSAA 2 cut(s) 69, 191
AhlI ACTAGT 1 cut(s) 709
AjnI CCWGG 3 cut(s) 54, 319, 840
AluBI AGCT 8 cut(s) 8, 20, 108, 204, 214, 273, 390, 437
AluI AGCT 8 cut(s) 8, 20, 108, 204, 214, 273, 390, 437
Alw21I GWGCWC 1 cut(s) 553
Alw44I GTGCAC 1 cut(s) 549
AlwI GGATC 2 cut(s) 460, 473
Aor13HI TCCGGA 1 cut(s) 32
AoxI GGCC 3 cut(s) 57, 223, 567
ApaLI GTGCAC 1 cut(s) 549
ApoI RAATTY 3 cut(s) 64, 98, 856
AspS9I GGNCC 2 cut(s) 58, 567
AsuC2I CCSGG 1 cut(s) 367
AsuHPI GGTGA 1 cut(s) 43
BaeGI GKGCMC 2 cut(s) 159, 553
BamHI GGATCC 1 cut(s) 465
BanI GGYRCC 2 cut(s) 154, 255
BanII GRGCYC 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 553
BccI CCATC 1 cut(s) 145
BceAI ACGGC 2 cut(s) 97, 625
BciT130I CCWGG 3 cut(s) 56, 321, 842
BcnI CCSGG 1 cut(s) 367
BcuI ACTAGT 1 cut(s) 709
BfaI CTAG 2 cut(s) 590, 710
BfmI CTRYAG 1 cut(s) 103
BfuAI ACCTGC 1 cut(s) 673
BisI GCNGC 1 cut(s) 226
BlsI GCNGC 1 cut(s) 227
BmcAI AGTACT 1 cut(s) 714
Bme1390I CCNGG 4 cut(s) 56, 321, 367, 842
BmgT120I GGNCC 2 cut(s) 58, 567
BmiI GGNNCC 5 cut(s) 60, 94, 156, 257, 467
BmrFI CCNGG 4 cut(s) 56, 321, 367, 842
BmsI GCATC 2 cut(s) 360, 880
BpuEI CTTGAG 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 367
BsaAI YACGTR 1 cut(s) 817
BsaJI CCNNGG 2 cut(s) 54, 220
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 2 cut(s) 854, 884
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 181, 252
Bse118I RCCGGY 1 cut(s) 893
Bse1I ACTGG 2 cut(s) 435, 739
BseAI TCCGGA 1 cut(s) 32
BseBI CCWGG 3 cut(s) 56, 321, 842
BseDI CCNNGG 2 cut(s) 54, 220
BseGI GGATG 1 cut(s) 375
BseLI CCNNNNNNNGG 3 cut(s) 142, 181, 252
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 2 cut(s) 435, 739
BseSI GKGCMC 2 cut(s) 159, 553
BsgI GTGCAG 1 cut(s) 334
Bsh1236I CGCG 1 cut(s) 908
BshFI GGCC 3 cut(s) 59, 225, 569
BshNI GGYRCC 2 cut(s) 154, 255
BsiHKAI GWGCWC 1 cut(s) 553
BsiSI CCGG 3 cut(s) 33, 366, 894
BslI CCNNNNNNNGG 3 cut(s) 142, 181, 252
BsmI GAATGC 2 cut(s) 519, 668
BsnI GGCC 3 cut(s) 59, 225, 569
Bsp1286I GDGCHC 3 cut(s) 95, 159, 553
Bsp13I TCCGGA 1 cut(s) 32
Bsp143I GATC 1 cut(s) 465
Bsp19I CCATGG 1 cut(s) 220
BspACI CCGC 3 cut(s) 226, 684, 908
BspANI GGCC 3 cut(s) 59, 225, 569
BspCNI CTCAG 1 cut(s) 473
BspEI TCCGGA 1 cut(s) 32
BspFNI CGCG 1 cut(s) 908
BspHI TCATGA 1 cut(s) 237
BspLI GGNNCC 5 cut(s) 60, 94, 156, 257, 467
BspMI ACCTGC 1 cut(s) 673
BspPI GGATC 2 cut(s) 460, 473
BspT107I GGYRCC 2 cut(s) 154, 255
BsrBI CCGCTC 1 cut(s) 228
BsrFI RCCGGY 1 cut(s) 893
BsrI ACTGG 2 cut(s) 435, 739
BssAI RCCGGY 1 cut(s) 893
BssECI CCNNGG 2 cut(s) 54, 220
BssMI GATC 1 cut(s) 465
BssNAI GTATAC 1 cut(s) 681
BssT1I CCWWGG 1 cut(s) 220
Bst1107I GTATAC 1 cut(s) 681
Bst2UI CCWGG 3 cut(s) 56, 321, 842
Bst4CI ACNGT 2 cut(s) 548, 717
Bst6I CTCTTC 1 cut(s) 173
BstAPI GCANNNNNTGC 1 cut(s) 665
BstBAI YACGTR 1 cut(s) 817
BstC8I GCNNGC 7 cut(s) 6, 110, 275, 392, 396, 724, 728
BstDEI CTNAG 1 cut(s) 460
BstDSI CCRYGG 1 cut(s) 220
BstEII GGTNACC 1 cut(s) 651
BstF5I GGATG 1 cut(s) 375
BstFNI CGCG 1 cut(s) 908
BstKTI GATC 1 cut(s) 468
BstMBI GATC 1 cut(s) 465
BstMWI GCNNNNNNNGC 3 cut(s) 90, 114, 665
BstNI CCWGG 3 cut(s) 56, 321, 842
BstPI GGTNACC 1 cut(s) 651
BstSCI CCNGG 4 cut(s) 54, 319, 365, 840
BstSFI CTRYAG 1 cut(s) 103
BstSLI GKGCMC 2 cut(s) 159, 553
BstSNI TACGTA 1 cut(s) 817
BstUI CGCG 1 cut(s) 908
BstX2I RGATCY 1 cut(s) 465
BstYI RGATCY 1 cut(s) 465
BstZ17I GTATAC 1 cut(s) 681
BsuRI GGCC 3 cut(s) 59, 225, 569
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 1 cut(s) 375
BveI ACCTGC 1 cut(s) 673
Cac8I GCNNGC 7 cut(s) 6, 110, 275, 392, 396, 724, 728
CciI TCATGA 1 cut(s) 237
Cfr10I RCCGGY 1 cut(s) 893
Cfr13I GGNCC 2 cut(s) 58, 567
CseI GACGC 1 cut(s) 914
Csp6I GTAC 5 cut(s) 628, 707, 713, 814, 871
CviAII CATG 7 cut(s) 125, 221, 238, 306, 336, 625, 691
CviQI GTAC 5 cut(s) 628, 707, 713, 814, 871
DdeI CTNAG 1 cut(s) 460
DpnI GATC 1 cut(s) 467
DpnII GATC 1 cut(s) 465
DraI TTTAAA 1 cut(s) 855
DraIII CACNNNGTG 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
Eco105I TACGTA 1 cut(s) 817
Eco130I CCWWGG 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 392
Eco91I GGTNACC 1 cut(s) 651
EcoO109I RGGNCCY 1 cut(s) 58
EcoO65I GGTNACC 1 cut(s) 651
EcoRI GAATTC 1 cut(s) 98
EcoRII CCWGG 3 cut(s) 54, 319, 840
EcoT14I CCWWGG 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 95
ErhI CCWWGG 1 cut(s) 220
FaeI CATG 7 cut(s) 128, 224, 241, 309, 339, 628, 694
FalI AAGNNNNNCTT 2 cut(s) 554, 586
FatI CATG 7 cut(s) 124, 220, 237, 305, 335, 624, 690
FblI GTMKAC 1 cut(s) 680
Fnu4HI GCNGC 1 cut(s) 226
FokI GGATG 1 cut(s) 382
FriOI GRGCYC 1 cut(s) 95
Fsp4HI GCNGC 1 cut(s) 226
FspBI CTAG 2 cut(s) 590, 710
GluI GCNGC 1 cut(s) 226
HaeIII GGCC 3 cut(s) 59, 225, 569
HapII CCGG 3 cut(s) 33, 366, 894
HgaI GACGC 1 cut(s) 914
Hin1II CATG 7 cut(s) 128, 224, 241, 309, 339, 628, 694
HindIII AAGCTT 4 cut(s) 18, 202, 388, 435
HinfI GANTC 2 cut(s) 36, 756
HpaII CCGG 3 cut(s) 33, 366, 894
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 7 cut(s) 357, 428, 551, 583, 681, 696, 871
Hpy188I TCNGA 3 cut(s) 97, 150, 880
Hpy188III TCNNGA 4 cut(s) 33, 195, 208, 238
Hpy8I GTNNAC 7 cut(s) 357, 428, 551, 583, 681, 696, 871
Hpy99I CGWCG 1 cut(s) 83
HpyCH4III ACNGT 2 cut(s) 548, 717
HpyCH4IV ACGT 3 cut(s) 698, 705, 816
HpyCH4V TGCA 6 cut(s) 4, 315, 422, 551, 602, 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 114, 665
HpyF3I CTNAG 1 cut(s) 460
HpySE526I ACGT 3 cut(s) 698, 705, 816
Hsp92II CATG 7 cut(s) 128, 224, 241, 309, 339, 628, 694
Kpn2I TCCGGA 1 cut(s) 32
Kzo9I GATC 1 cut(s) 465
LmnI GCTCC 2 cut(s) 13, 98
LweI GCATC 2 cut(s) 360, 880
MaeI CTAG 2 cut(s) 590, 710
MaeII ACGT 3 cut(s) 698, 705, 816
MaeIII GTNAC 3 cut(s) 331, 651, 817
MalI GATC 1 cut(s) 467
MbiI CCGCTC 1 cut(s) 228
MboI GATC 1 cut(s) 465
MboII GAAGA 5 cut(s) 124, 160, 794, 829, 860
MflI RGATCY 1 cut(s) 465
MhlI GDGCHC 3 cut(s) 95, 159, 553
MluCI AATT 6 cut(s) 42, 64, 98, 415, 603, 856
MlyI GAGTC 1 cut(s) 30
MnlI CCTC 6 cut(s) 34, 169, 269, 373, 642, 838
MroI TCCGGA 1 cut(s) 32
MseI TTAA 2 cut(s) 701, 854
MslI CAYNNNNRTG 1 cut(s) 310
MspA1I CMGCKG 1 cut(s) 108
MspI CCGG 3 cut(s) 33, 366, 894
MspR9I CCNGG 4 cut(s) 56, 321, 367, 842
Mva1269I GAATGC 2 cut(s) 519, 668
MvaI CCWGG 3 cut(s) 56, 321, 842
MvnI CGCG 1 cut(s) 908
MwoI GCNNNNNNNGC 3 cut(s) 90, 114, 665
NciI CCSGG 1 cut(s) 367
NcoI CCATGG 1 cut(s) 220
NdeII GATC 1 cut(s) 465
NlaIII CATG 7 cut(s) 128, 224, 241, 309, 339, 628, 694
NlaIV GGNNCC 5 cut(s) 60, 94, 156, 257, 467
NmeAIII GCCGAG 1 cut(s) 93
PagI TCATGA 1 cut(s) 237
PctI GAATGC 2 cut(s) 519, 668
PfeI GAWTC 1 cut(s) 756
PflMI CCANNNNNTGG 1 cut(s) 142
PfoI TCCNGGA 3 cut(s) 319, 365, 840
PkrI GCNGC 1 cut(s) 227
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
Ppu21I YACGTR 1 cut(s) 817
Psp6I CCWGG 3 cut(s) 54, 319, 840
PspEI GGTNACC 1 cut(s) 651
PspGI CCWGG 3 cut(s) 54, 319, 840
PspN4I GGNNCC 5 cut(s) 60, 94, 156, 257, 467
PspPI GGNCC 2 cut(s) 58, 567
PsuI RGATCY 1 cut(s) 465
PvuII CAGCTG 1 cut(s) 108
RsaI GTAC 5 cut(s) 629, 708, 714, 815, 872
RsaNI GTAC 5 cut(s) 628, 707, 713, 814, 871
RseI CAYNNNNRTG 1 cut(s) 310
SaqAI TTAA 2 cut(s) 701, 854
SatI GCNGC 1 cut(s) 226
Sau3AI GATC 1 cut(s) 465
Sau96I GGNCC 2 cut(s) 58, 567
ScaI AGTACT 1 cut(s) 714
SchI GAGTC 1 cut(s) 30
ScrFI CCNGG 4 cut(s) 56, 321, 367, 842
SduI GDGCHC 3 cut(s) 95, 159, 553
SfaNI GCATC 2 cut(s) 360, 880
SfcI CTRYAG 1 cut(s) 103
SmiI ATTTAAAT 1 cut(s) 855
SmiMI CAYNNNNRTG 1 cut(s) 310
SmlI CTYRAG 1 cut(s) 491
SmoI CTYRAG 1 cut(s) 491
SnaBI TACGTA 1 cut(s) 817
SpeI ACTAGT 1 cut(s) 709
Sse9I AATT 6 cut(s) 42, 64, 98, 415, 603, 856
SsiI CCGC 3 cut(s) 226, 684, 908
SspMI CTAG 2 cut(s) 590, 710
StyD4I CCNGG 4 cut(s) 54, 319, 365, 840
StyI CCWWGG 1 cut(s) 220
SwaI ATTTAAAT 1 cut(s) 855
TaaI ACNGT 2 cut(s) 548, 717
TaiI ACGT 3 cut(s) 701, 708, 819
TaqI TCGA 3 cut(s) 194, 209, 556
TasI AATT 6 cut(s) 42, 64, 98, 415, 603, 856
TatI WGTACW 2 cut(s) 712, 870
TauI GCSGC 1 cut(s) 228
TfiI GAWTC 1 cut(s) 756
Tru1I TTAA 2 cut(s) 701, 854
Tru9I TTAA 2 cut(s) 701, 854
TspDTI ATGAA 4 cut(s) 520, 604, 613, 899
TspGWI ACGGA 1 cut(s) 460
Van91I CCANNNNNTGG 1 cut(s) 142
VneI GTGCAC 1 cut(s) 549
XapI RAATTY 3 cut(s) 64, 98, 856
XmiI GTMKAC 1 cut(s) 680
XspI CTAG 2 cut(s) 590, 710
ZrmI AGTACT 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.