Rorug06G0451300

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004430
Physical Location & Seq
Forward (+)
58064444 .. 58067084
2641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0451300.1

Sequence Viewer

Length: 1023 bp
ATGGAGCGGAATTCAGCTGCGGTTCACTCCCAAATGACGCCGTTGATGTTCGGGTCATCGTGGCAAGGTGATCACATGAAGCGAAGCCCGCCGCAGTTGGAAATCGACGAGTGGATCAACCATGGGGTCGCAGCTGATAACCACGGCGAGTTTCGGCCGGACCGGAGTTTCGCCGAAACGGACGGCTTTTTCGGCGACGTTTGCTCCGGTGACCTCAGCTTCGCTTTCAAGAACCGGGACATGATGAATGGTTTTTCAAGCAGTGGACTGACTGAAACCCTACTAAGCCCTCAGAATCTCACCCCCAAGAATTCCAGCCTCTCAGCAACCATGGATTCCCAGTCATCGATATGTGTTGGAACTCCCACATCGGCCGCTAACCCAATTGGTAGAGATAACCAAGCAAGAGGAGCCACCAGTGGTTCCTCTGGAGATCAATCAGATGACGATGATTTTGAGATAGAAGCCGGTCCATGTGGAGACAGTACAGACCCTCTTGATATTAAACGTATCAGAAGGATGGTCTCGAACAGGGAGTCTGCTAGGCGATCAAGAAGAAGAAAGCAACAACATTTGCAAGAACTTGAGGGGCAGGTTGATATACTGAGAGTAGAAAATTCAACCTTATTAATTCAGCTTACAGATGCTTCTCAACAGTACCGTGATGCTGATACAAACAATAGAGTGCTGAAATCAGATGTGGAAGCTTTGAGAGCTAAGGTGAAGCTAGCTGAAGATATGGTTGCTCGCGGCTCTTTAACCTCTAGCCTTAACCAACTTCTTCAAGGTCATTTAACCACACCACAACCACTTAATCCTCATACTAATTTGCGCGGTGTAGCACATGTGTCGCCAACCATTACCATTCATGGAGAGGATACCTCATATGGTGGAATAGCAGTCTCTGGTCAGAACTCGGAAATGCTTGTTGGACTCGGAAATGCTGCTGGTTTGACTAATAGCAATATGAGTACCAGGATCATGAGTGATGCTGTAAGCTGTGTGTCGACTGATATGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

340

Amino Acids

36.87

Weight (kDa)

5.19

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bZIP_1 PF00170 168 - 219 7.8e-14 bZIP transcription factor
bZIP_2 PF07716 169 - 214 6.7e-08 Basic region leucine zipper
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 583
AccBSI CCGCTC 1 cut(s) 7
AccI GTMKAC 1 cut(s) 1007
AccII CGCG 2 cut(s) 750, 834
AciI CCGC 7 cut(s) 7, 20, 89, 92, 375, 750, 834
AclWI GGATC 2 cut(s) 122, 986
AcoI YGGCCR 2 cut(s) 155, 372
AcsI RAATTY 3 cut(s) 10, 310, 616
AcuI CTGAAG 1 cut(s) 753
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 3 cut(s) 487, 659, 973
AflIII ACRYGT 1 cut(s) 844
AgsI TTSAA 4 cut(s) 229, 258, 621, 785
AjnI CCWGG 1 cut(s) 974
AjuI GAANNNNNNNTTGG 2 cut(s) 912, 944
AluBI AGCT 9 cut(s) 17, 134, 219, 637, 707, 716, 727, 731, 999
AluI AGCT 9 cut(s) 17, 134, 219, 637, 707, 716, 727, 731, 999
Alw26I GTCTC 3 cut(s) 474, 529, 907
AlwI GGATC 2 cut(s) 122, 986
AlwNI CAGNNNCTG 1 cut(s) 905
AoxI GGCC 2 cut(s) 155, 372
ApeKI GCWGC 3 cut(s) 17, 131, 944
ApoI RAATTY 3 cut(s) 10, 310, 616
AseI ATTAAT 1 cut(s) 629
AspLEI GCGC 1 cut(s) 834
AspS9I GGNCC 2 cut(s) 160, 470
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 4 cut(s) 80, 221, 292, 733
AsuNHI GCTAGC 1 cut(s) 727
AvaII GGWCC 2 cut(s) 160, 470
BbvCI CCTCAGC 1 cut(s) 215
BbvI GCAGC 3 cut(s) 4, 143, 931
BccI CCATC 1 cut(s) 514
BceAI ACGGC 3 cut(s) 25, 160, 199
BcgI CGANNNNNNTGC 2 cut(s) 831, 865
BciT130I CCWGG 1 cut(s) 976
BciVI GTATCC 1 cut(s) 871
BclI TGATCA 1 cut(s) 70
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 3 cut(s) 474, 529, 907
BfaI CTAG 3 cut(s) 543, 728, 765
BfuAI ACCTGC 1 cut(s) 583
BfuI GTATCC 1 cut(s) 871
BisI GCNGC 6 cut(s) 18, 92, 132, 375, 751, 945
BlsI GCNGC 6 cut(s) 19, 93, 133, 376, 752, 946
Bme1390I CCNGG 2 cut(s) 236, 976
Bme18I GGWCC 2 cut(s) 160, 470
BmgT120I GGNCC 2 cut(s) 160, 470
BmiI GGNNCC 2 cut(s) 412, 424
BmrFI CCNGG 2 cut(s) 236, 976
BmrI ACTGGG 1 cut(s) 334
BmsI GCATC 3 cut(s) 634, 655, 979
BmtI GCTAGC 1 cut(s) 731
BmuI ACTGGG 1 cut(s) 334
BplI GAGNNNNNCTC 2 cut(s) 866, 898
BpmI CTGGAG 1 cut(s) 450
Bpu10I CCTNAGC 2 cut(s) 215, 717
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 1 cut(s) 236
Bsa29I ATCGAT 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 38
BsaI GGTCTC 1 cut(s) 529
BsaJI CCNNGG 3 cut(s) 121, 142, 330
BsaWI WCCGGW 2 cut(s) 162, 206
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 2 cut(s) 340, 417
BseBI CCWGG 1 cut(s) 976
BseCI ATCGAT 1 cut(s) 347
BseDI CCNNGG 3 cut(s) 121, 142, 330
BseGI GGATG 1 cut(s) 525
BseMII CTCAG 4 cut(s) 229, 305, 336, 596
BseNI ACTGG 2 cut(s) 340, 417
BseRI GAGGAG 1 cut(s) 423
BseX3I CGGCCG 2 cut(s) 155, 372
BseXI GCAGC 3 cut(s) 4, 143, 931
Bsh1236I CGCG 2 cut(s) 750, 834
Bsh1285I CGRYCG 2 cut(s) 158, 375
BshFI GGCC 2 cut(s) 157, 374
BshVI ATCGAT 1 cut(s) 347
BsiEI CGRYCG 2 cut(s) 158, 375
BsiSI CCGG 5 cut(s) 158, 163, 207, 235, 468
BslFI GGGAC 1 cut(s) 251
BsmAI GTCTC 3 cut(s) 474, 529, 907
BsmFI GGGAC 1 cut(s) 251
BsnI GGCC 2 cut(s) 157, 374
Bso31I GGTCTC 1 cut(s) 529
Bsp143I GATC 5 cut(s) 70, 114, 433, 548, 978
Bsp19I CCATGG 2 cut(s) 121, 330
BspACI CCGC 7 cut(s) 7, 20, 89, 92, 375, 750, 834
BspANI GGCC 2 cut(s) 157, 374
BspCNI CTCAG 4 cut(s) 228, 304, 335, 597
BspDI ATCGAT 1 cut(s) 347
BspFNI CGCG 2 cut(s) 750, 834
BspHI TCATGA 1 cut(s) 981
BspLI GGNNCC 2 cut(s) 412, 424
BspMI ACCTGC 1 cut(s) 583
BspOI GCTAGC 1 cut(s) 731
BspPI GGATC 2 cut(s) 122, 986
BspTNI GGTCTC 1 cut(s) 529
BsrBI CCGCTC 1 cut(s) 7
BsrFI RCCGGY 1 cut(s) 467
BsrI ACTGG 2 cut(s) 340, 417
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 3 cut(s) 121, 142, 330
BssMI GATC 5 cut(s) 70, 114, 433, 548, 978
BssNI GRCGYC 1 cut(s) 38
BssT1I CCWWGG 2 cut(s) 121, 330
Bst2UI CCWGG 1 cut(s) 976
Bst4CI ACNGT 3 cut(s) 485, 657, 662
BstACI GRCGYC 1 cut(s) 38
BstC8I GCNNGC 3 cut(s) 89, 729, 748
BstDEI CTNAG 6 cut(s) 215, 284, 291, 322, 605, 717
BstDSI CCRYGG 3 cut(s) 121, 142, 330
BstEII GGTNACC 1 cut(s) 209
BstF5I GGATG 1 cut(s) 525
BstFNI CGCG 2 cut(s) 750, 834
BstHHI GCGC 1 cut(s) 834
BstKTI GATC 5 cut(s) 73, 117, 436, 551, 981
BstMAI GTCTC 3 cut(s) 474, 529, 907
BstMBI GATC 5 cut(s) 70, 114, 433, 548, 978
BstMCI CGRYCG 2 cut(s) 158, 375
BstMWI GCNNNNNNNGC 5 cut(s) 88, 192, 201, 410, 713
BstNI CCWGG 1 cut(s) 976
BstNSI RCATGY 1 cut(s) 848
BstPI GGTNACC 1 cut(s) 209
BstSCI CCNGG 2 cut(s) 234, 974
BstUI CGCG 2 cut(s) 750, 834
BstV1I GCAGC 3 cut(s) 4, 143, 931
BstZI CGGCCG 2 cut(s) 155, 372
Bsu15I ATCGAT 1 cut(s) 347
BsuI GTATCC 1 cut(s) 871
BsuRI GGCC 2 cut(s) 157, 374
BsuTUI ATCGAT 1 cut(s) 347
BtgI CCRYGG 3 cut(s) 121, 142, 330
BtsCI GGATG 1 cut(s) 525
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 2 cut(s) 268, 424
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 3 cut(s) 89, 729, 748
CaiI CAGNNNCTG 1 cut(s) 905
CciI TCATGA 1 cut(s) 981
CfoI GCGC 1 cut(s) 834
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 2 cut(s) 160, 470
ClaI ATCGAT 1 cut(s) 347
CpoI CGGWCCG 1 cut(s) 160
CseI GACGC 1 cut(s) 46
Csp6I GTAC 3 cut(s) 486, 658, 972
CspI CGGWCCG 1 cut(s) 160
CviAII CATG 8 cut(s) 76, 122, 241, 331, 474, 845, 869, 982
CviQI GTAC 3 cut(s) 486, 658, 972
DdeI CTNAG 6 cut(s) 215, 284, 291, 322, 605, 717
DpnI GATC 5 cut(s) 72, 116, 435, 550, 980
DpnII GATC 5 cut(s) 70, 114, 433, 548, 978
EaeI YGGCCR 2 cut(s) 155, 372
EagI CGGCCG 2 cut(s) 155, 372
EclXI CGGCCG 2 cut(s) 155, 372
Eco130I CCWWGG 2 cut(s) 121, 330
Eco31I GGTCTC 1 cut(s) 529
Eco47I GGWCC 2 cut(s) 160, 470
Eco52I CGGCCG 2 cut(s) 155, 372
Eco57I CTGAAG 1 cut(s) 753
Eco91I GGTNACC 1 cut(s) 209
EcoO65I GGTNACC 1 cut(s) 209
EcoRI GAATTC 2 cut(s) 10, 310
EcoRII CCWGG 1 cut(s) 974
EcoT14I CCWWGG 2 cut(s) 121, 330
ErhI CCWWGG 2 cut(s) 121, 330
FaeI CATG 8 cut(s) 79, 125, 244, 334, 477, 848, 872, 985
FaqI GGGAC 1 cut(s) 251
FatI CATG 8 cut(s) 75, 121, 240, 330, 473, 844, 868, 981
FauI CCCGC 1 cut(s) 96
FauNDI CATATG 1 cut(s) 886
FbaI TGATCA 1 cut(s) 70
FblI GTMKAC 1 cut(s) 1007
Fnu4HI GCNGC 6 cut(s) 18, 92, 132, 375, 751, 945
FokI GGATG 1 cut(s) 532
Fsp4HI GCNGC 6 cut(s) 18, 92, 132, 375, 751, 945
FspBI CTAG 3 cut(s) 543, 728, 765
GlaI GCGC 1 cut(s) 833
GluI GCNGC 6 cut(s) 18, 92, 132, 375, 751, 945
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 2 cut(s) 157, 374
HapII CCGG 5 cut(s) 158, 163, 207, 235, 468
HgaI GACGC 1 cut(s) 46
HhaI GCGC 1 cut(s) 834
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 8 cut(s) 79, 125, 244, 334, 477, 848, 872, 985
Hin6I GCGC 1 cut(s) 832
HinP1I GCGC 1 cut(s) 832
HincII GTYRAC 1 cut(s) 1008
HindII GTYRAC 1 cut(s) 1008
HindIII AAGCTT 1 cut(s) 705
HinfI GANTC 4 cut(s) 295, 335, 536, 933
HpaII CCGG 5 cut(s) 158, 163, 207, 235, 468
HphI GGTGA 4 cut(s) 80, 221, 292, 733
Hpy166II GTNNAC 3 cut(s) 25, 266, 1008
Hpy188I TCNGA 7 cut(s) 294, 442, 515, 697, 912, 919, 938
Hpy188III TCNNGA 6 cut(s) 229, 429, 497, 526, 552, 982
Hpy8I GTNNAC 3 cut(s) 25, 266, 1008
Hpy99I CGWCG 2 cut(s) 110, 200
HpyAV CCTTC 1 cut(s) 510
HpyCH4III ACNGT 3 cut(s) 485, 657, 662
HpyCH4IV ACGT 2 cut(s) 198, 508
HpyCH4V TGCA 1 cut(s) 577
HpyF10VI GCNNNNNNNGC 5 cut(s) 88, 192, 201, 410, 713
HpyF3I CTNAG 6 cut(s) 215, 284, 291, 322, 605, 717
HpySE526I ACGT 2 cut(s) 198, 508
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 8 cut(s) 79, 125, 244, 334, 477, 848, 872, 985
HspAI GCGC 1 cut(s) 832
Ksp22I TGATCA 1 cut(s) 70
Kzo9I GATC 5 cut(s) 70, 114, 433, 548, 978
LmnI GCTCC 3 cut(s) 4, 209, 410
Lsp1109I GCAGC 3 cut(s) 4, 143, 931
LweI GCATC 3 cut(s) 634, 655, 979
MaeI CTAG 3 cut(s) 543, 728, 765
MaeII ACGT 2 cut(s) 198, 508
MaeIII GTNAC 1 cut(s) 209
MalI GATC 5 cut(s) 72, 116, 435, 550, 980
MbiI CCGCTC 1 cut(s) 7
MboI GATC 5 cut(s) 70, 114, 433, 548, 978
MboII GAAGA 4 cut(s) 567, 570, 746, 773
MfeI CAATTG 1 cut(s) 384
MluCI AATT 6 cut(s) 10, 310, 384, 616, 630, 826
MlyI GAGTC 2 cut(s) 545, 927
MmeI TCCRAC 3 cut(s) 78, 337, 910
MseI TTAA 6 cut(s) 504, 629, 758, 771, 794, 813
MslI CAYNNNNRTG 1 cut(s) 349
MspA1I CMGCKG 2 cut(s) 17, 134
MspI CCGG 5 cut(s) 158, 163, 207, 235, 468
MspR9I CCNGG 2 cut(s) 236, 976
MunI CAATTG 1 cut(s) 384
MvaI CCWGG 1 cut(s) 976
MvnI CGCG 2 cut(s) 750, 834
MwoI GCNNNNNNNGC 5 cut(s) 88, 192, 201, 410, 713
NciI CCSGG 1 cut(s) 236
NcoI CCATGG 2 cut(s) 121, 330
NdeI CATATG 1 cut(s) 886
NdeII GATC 5 cut(s) 70, 114, 433, 548, 978
NheI GCTAGC 1 cut(s) 727
NlaIII CATG 8 cut(s) 79, 125, 244, 334, 477, 848, 872, 985
NlaIV GGNNCC 2 cut(s) 412, 424
NmuCI GTSAC 1 cut(s) 209
NspI RCATGY 1 cut(s) 848
PagI TCATGA 1 cut(s) 981
PciI ACATGT 1 cut(s) 844
PfeI GAWTC 2 cut(s) 295, 335
PkrI GCNGC 6 cut(s) 19, 93, 133, 376, 752, 946
PleI GAGTC 2 cut(s) 544, 927
PpsI GAGTC 2 cut(s) 544, 927
PscI ACATGT 1 cut(s) 844
PshBI ATTAAT 1 cut(s) 629
Psp6I CCWGG 1 cut(s) 974
PspEI GGTNACC 1 cut(s) 209
PspGI CCWGG 1 cut(s) 974
PspN4I GGNNCC 2 cut(s) 412, 424
PspPI GGNCC 2 cut(s) 160, 470
PstNI CAGNNNCTG 1 cut(s) 905
PvuII CAGCTG 2 cut(s) 17, 134
RsaI GTAC 3 cut(s) 487, 659, 973
RsaNI GTAC 3 cut(s) 486, 658, 972
RseI CAYNNNNRTG 1 cut(s) 349
Rsr2I CGGWCCG 1 cut(s) 160
RsrII CGGWCCG 1 cut(s) 160
SalI GTCGAC 1 cut(s) 1006
SaqAI TTAA 6 cut(s) 504, 629, 758, 771, 794, 813
SatI GCNGC 6 cut(s) 18, 92, 132, 375, 751, 945
Sau3AI GATC 5 cut(s) 70, 114, 433, 548, 978
Sau96I GGNCC 2 cut(s) 160, 470
SchI GAGTC 2 cut(s) 545, 927
ScrFI CCNGG 2 cut(s) 236, 976
SfaNI GCATC 3 cut(s) 634, 655, 979
SinI GGWCC 2 cut(s) 160, 470
SmiMI CAYNNNNRTG 1 cut(s) 349
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
Sse9I AATT 6 cut(s) 10, 310, 384, 616, 630, 826
SsiI CCGC 7 cut(s) 7, 20, 89, 92, 375, 750, 834
SspMI CTAG 3 cut(s) 543, 728, 765
StyD4I CCNGG 2 cut(s) 234, 974
StyI CCWWGG 2 cut(s) 121, 330
TaaI ACNGT 3 cut(s) 485, 657, 662
TaiI ACGT 2 cut(s) 201, 511
TaqI TCGA 4 cut(s) 105, 347, 527, 1007
TasI AATT 6 cut(s) 10, 310, 384, 616, 630, 826
TatI WGTACW 1 cut(s) 485
TauI GCSGC 3 cut(s) 94, 377, 753
TfiI GAWTC 2 cut(s) 295, 335
Tru1I TTAA 6 cut(s) 504, 629, 758, 771, 794, 813
Tru9I TTAA 6 cut(s) 504, 629, 758, 771, 794, 813
TscAI CASTG 2 cut(s) 268, 424
TseFI GTSAC 1 cut(s) 209
TseI GCWGC 3 cut(s) 17, 131, 944
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 3 cut(s) 92, 260, 857
TspGWI ACGGA 1 cut(s) 194
TspRI CASTG 2 cut(s) 268, 424
VpaK11BI GGWCC 2 cut(s) 160, 470
VspI ATTAAT 1 cut(s) 629
XapI RAATTY 3 cut(s) 10, 310, 616
XceI RCATGY 1 cut(s) 848
XmiI GTMKAC 1 cut(s) 1007
XspI CTAG 3 cut(s) 543, 728, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.