MD14G1210800.v1.1

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004430
Physical Location & Seq
Forward (+)
29635412 .. 29637591
2180 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1210800.v1.1.491

Sequence Viewer

Length: 867 bp
ATGGCGACGGACCTTCAGGAACACTTCAACACGTCGGTCAACTCGCCGATCCCGAGCCTCCCAGCTATAATCAACGGTCAAGATCAGAGCTTTTCCGTCGTCATCTTGCCGGAGTGCCATGGTATCTGGTACGACGCCGTTCTGGTGGTTCCTGCAGTCGTTTTCGTGGTTTACTTAGCGTTCCACGCCAAGAAGAACGTCATCAAGCTCTCCAATGGAAGATCATACATCATGATTTCCTATTATGCCCTTGTGTGGGTCGCAAGCCTTCTCAACCTCGCCTGGTGCGCTCTTCAGGTTCGCATGGCAGTGCTCAGGTGGGAAGGAGGTCGCTTGGAATTTGTTGTCGTTGTTTACGGGTTCTGCAATGCTGGGATTGAGATTAGCTTGGTGGGTTTCTTGCTCCAGGGGAATTATGCAAGTGGGGTTGAAGCTTTAGCTCATACTTTCCTTGTTTCTGGGATTGCTGTTGGTGTAGACACATTGCTTAAGGTAATCTATGTATTCGGGTTTGGGGTACCGCTGTTCATCGTTGGCATCGGAAGTACATTTGGAGTGAAGTGGGGCTTGTGGCTCATCCACAAATTATTGCTTACTGCACTTTATGGCTTCATATTGTTTGTGCATTTTTCAAAATCGAGAGAGAAGTTACCTCCCAGACCAGCATCCTACAACTACATCGTCGTAATGTTTGTACTTTGTGCTCTGGCGTCGTTTGGTTGCGGGCTTGCTGGAATTGGAGCTAGTTTCGGAATCTGGTTGTACAATTTGACAGATATATGCTACCACACACTCTATCTTCCTTCTCTGTACATGATTTTTGTAGCCGATTTTTTCCAGGCGATCATTCTTCTCAATAATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

289

Amino Acids

31.74

Weight (kDa)

7.63

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_40 PF10160 38 - 280 3.4e-84 Predicted membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 517
AccB1I GGYRCC 1 cut(s) 517
AccI GTMKAC 1 cut(s) 477
AciI CCGC 2 cut(s) 521, 723
AclWI GGATC 1 cut(s) 43
AcsI RAATTY 1 cut(s) 338
AcuI CTGAAG 1 cut(s) 278
AcyI GRCGYC 2 cut(s) 135, 710
AfaI GTAC 6 cut(s) 131, 519, 547, 696, 764, 812
AfiI CCNNNNNNNGG 2 cut(s) 255, 256
AflII CTTAAG 1 cut(s) 488
AflIII ACRYGT 1 cut(s) 30
AgsI TTSAA 3 cut(s) 28, 431, 633
AjiI CACGTC 1 cut(s) 33
AjnI CCWGG 3 cut(s) 281, 405, 837
AluBI AGCT 7 cut(s) 65, 90, 208, 387, 434, 440, 743
AluI AGCT 7 cut(s) 65, 90, 208, 387, 434, 440, 743
Alw21I GWGCWC 2 cut(s) 315, 706
AlwI GGATC 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 52
ApoI RAATTY 1 cut(s) 338
Asp718I GGTACC 1 cut(s) 517
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 10
AvaI CYCGRG 1 cut(s) 52
AvaII GGWCC 1 cut(s) 10
BaeI ACNNNNGTAYC 2 cut(s) 509, 542
BanI GGYRCC 1 cut(s) 517
Bbv12I GWGCWC 2 cut(s) 315, 706
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 3 cut(s) 283, 407, 839
BfaI CTAG 1 cut(s) 744
BfmI CTRYAG 1 cut(s) 153
BfrI CTTAAG 1 cut(s) 488
Bme1390I CCNGG 3 cut(s) 283, 407, 839
Bme18I GGWCC 1 cut(s) 10
BmeT110I CYCGRG 1 cut(s) 52
BmgBI CACGTC 1 cut(s) 33
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 2 cut(s) 150, 519
BmrFI CCNGG 3 cut(s) 283, 407, 839
BmsI GCATC 2 cut(s) 546, 674
BpmI CTGGAG 1 cut(s) 389
Bpu10I CCTNAGC 1 cut(s) 314
BsaHI GRCGYC 2 cut(s) 135, 710
BsaJI CCNNGG 2 cut(s) 118, 406
Bsc4I CCNNNNNNNGG 2 cut(s) 255, 256
Bse3DI GCAATG 2 cut(s) 373, 482
BseBI CCWGG 3 cut(s) 283, 407, 839
BseDI CCNNGG 2 cut(s) 118, 406
BseGI GGATG 2 cut(s) 576, 665
BseLI CCNNNNNNNGG 2 cut(s) 255, 256
BseMI GCAATG 2 cut(s) 373, 482
BseMII CTCAG 1 cut(s) 328
BseYI CCCAGC 2 cut(s) 61, 371
BsgI GTGCAG 1 cut(s) 582
BshNI GGYRCC 1 cut(s) 517
BsiHKAI GWGCWC 2 cut(s) 315, 706
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 255, 256
BsoBI CYCGRG 1 cut(s) 52
Bsp1286I GDGCHC 2 cut(s) 315, 706
Bsp1407I TGTACA 2 cut(s) 762, 810
Bsp143I GATC 4 cut(s) 48, 82, 221, 843
Bsp19I CCATGG 1 cut(s) 118
BspACI CCGC 2 cut(s) 521, 723
BspCNI CTCAG 1 cut(s) 327
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 2 cut(s) 150, 519
BspMAI CTGCAG 1 cut(s) 157
BspPI GGATC 1 cut(s) 43
BspQI GCTCTTC 1 cut(s) 297
BspT107I GGYRCC 1 cut(s) 517
BspTI CTTAAG 1 cut(s) 488
BsrDI GCAATG 2 cut(s) 373, 482
BsrGI TGTACA 2 cut(s) 762, 810
BssECI CCNNGG 2 cut(s) 118, 406
BssMI GATC 4 cut(s) 48, 82, 221, 843
BssNI GRCGYC 2 cut(s) 135, 710
BssT1I CCWWGG 1 cut(s) 118
Bst2UI CCWGG 3 cut(s) 283, 407, 839
Bst4CI ACNGT 1 cut(s) 77
Bst6I CTCTTC 1 cut(s) 297
BstACI GRCGYC 2 cut(s) 135, 710
BstAFI CTTAAG 1 cut(s) 488
BstAUI TGTACA 2 cut(s) 762, 810
BstC8I GCNNGC 3 cut(s) 265, 725, 729
BstDEI CTNAG 2 cut(s) 175, 314
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 2 cut(s) 576, 665
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 4 cut(s) 51, 85, 224, 846
BstMBI GATC 4 cut(s) 48, 82, 221, 843
BstMWI GCNNNNNNNGC 2 cut(s) 185, 287
BstNI CCWGG 3 cut(s) 283, 407, 839
BstSCI CCNGG 3 cut(s) 281, 405, 837
BstSFI CTRYAG 1 cut(s) 153
BtgI CCRYGG 1 cut(s) 118
BtrI CACGTC 1 cut(s) 33
BtsCI GGATG 2 cut(s) 576, 665
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 3 cut(s) 265, 725, 729
CciI TCATGA 1 cut(s) 231
CfoI GCGC 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 10
CseI GACGC 2 cut(s) 143, 699
Csp6I GTAC 6 cut(s) 130, 518, 546, 695, 763, 811
CviAII CATG 4 cut(s) 119, 232, 304, 814
CviQI GTAC 6 cut(s) 130, 518, 546, 695, 763, 811
DdeI CTNAG 2 cut(s) 175, 314
DpnI GATC 4 cut(s) 50, 84, 223, 845
DpnII GATC 4 cut(s) 48, 82, 221, 843
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 278
Eco88I CYCGRG 1 cut(s) 52
EcoRII CCWGG 3 cut(s) 281, 405, 837
EcoT14I CCWWGG 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 4 cut(s) 122, 235, 307, 817
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FatI CATG 4 cut(s) 118, 231, 303, 813
FauI CCCGC 1 cut(s) 716
FblI GTMKAC 1 cut(s) 477
FokI GGATG 2 cut(s) 563, 652
FspBI CTAG 1 cut(s) 744
GlaI GCGC 1 cut(s) 289
GsaI CCCAGC 2 cut(s) 65, 375
GsuI CTGGAG 1 cut(s) 389
HapII CCGG 1 cut(s) 110
HgaI GACGC 2 cut(s) 143, 699
HhaI GCGC 1 cut(s) 290
Hin1I GRCGYC 2 cut(s) 135, 710
Hin1II CATG 4 cut(s) 122, 235, 307, 817
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 432
HinfI GANTC 1 cut(s) 753
HpaII CCGG 1 cut(s) 110
Hpy166II GTNNAC 4 cut(s) 40, 172, 355, 478
Hpy188I TCNGA 3 cut(s) 87, 542, 752
Hpy188III TCNNGA 5 cut(s) 17, 52, 80, 232, 639
Hpy8I GTNNAC 4 cut(s) 40, 172, 355, 478
Hpy99I CGWCG 6 cut(s) 10, 37, 101, 137, 686, 715
HpyAV CCTTC 4 cut(s) 23, 278, 317, 813
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4IV ACGT 2 cut(s) 32, 198
HpyCH4V TGCA 5 cut(s) 155, 366, 419, 599, 625
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 287
HpyF3I CTNAG 2 cut(s) 175, 314
HpySE526I ACGT 2 cut(s) 32, 198
Hsp92I GRCGYC 2 cut(s) 135, 710
Hsp92II CATG 4 cut(s) 122, 235, 307, 817
HspAI GCGC 1 cut(s) 288
KpnI GGTACC 1 cut(s) 521
Kzo9I GATC 4 cut(s) 48, 82, 221, 843
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 2 cut(s) 408, 740
LweI GCATC 2 cut(s) 546, 674
MaeI CTAG 1 cut(s) 744
MaeII ACGT 2 cut(s) 32, 198
MaeIII GTNAC 1 cut(s) 648
MalI GATC 4 cut(s) 50, 84, 223, 845
MboI GATC 4 cut(s) 48, 82, 221, 843
MboII GAAGA 5 cut(s) 205, 231, 284, 791, 842
MhlI GDGCHC 2 cut(s) 315, 706
MluCI AATT 5 cut(s) 338, 412, 584, 735, 766
MnlI CCTC 4 cut(s) 68, 287, 320, 663
MseI TTAA 2 cut(s) 489, 865
MslI CAYNNNNRTG 1 cut(s) 308
MspA1I CMGCKG 1 cut(s) 523
MspCI CTTAAG 1 cut(s) 488
MspI CCGG 1 cut(s) 110
MspR9I CCNGG 3 cut(s) 283, 407, 839
MvaI CCWGG 3 cut(s) 283, 407, 839
MwoI GCNNNNNNNGC 2 cut(s) 185, 287
NcoI CCATGG 1 cut(s) 118
NdeII GATC 4 cut(s) 48, 82, 221, 843
NlaIII CATG 4 cut(s) 122, 235, 307, 817
NlaIV GGNNCC 2 cut(s) 150, 519
PagI TCATGA 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PfeI GAWTC 1 cut(s) 753
Psp6I CCWGG 3 cut(s) 281, 405, 837
PspFI CCCAGC 2 cut(s) 61, 371
PspGI CCWGG 3 cut(s) 281, 405, 837
PspN4I GGNNCC 2 cut(s) 150, 519
PspPI GGNCC 1 cut(s) 10
PstI CTGCAG 1 cut(s) 157
RsaI GTAC 6 cut(s) 131, 519, 547, 696, 764, 812
RsaNI GTAC 6 cut(s) 130, 518, 546, 695, 763, 811
RseI CAYNNNNRTG 1 cut(s) 308
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 2 cut(s) 489, 865
Sau3AI GATC 4 cut(s) 48, 82, 221, 843
Sau96I GGNCC 1 cut(s) 10
ScrFI CCNGG 3 cut(s) 283, 407, 839
SduI GDGCHC 2 cut(s) 315, 706
SfaNI GCATC 2 cut(s) 546, 674
SfcI CTRYAG 1 cut(s) 153
SinI GGWCC 1 cut(s) 10
SmiMI CAYNNNNRTG 1 cut(s) 308
SmlI CTYRAG 1 cut(s) 488
SmoI CTYRAG 1 cut(s) 488
Sse9I AATT 5 cut(s) 338, 412, 584, 735, 766
SsiI CCGC 2 cut(s) 521, 723
SspMI CTAG 1 cut(s) 744
StyD4I CCNGG 3 cut(s) 281, 405, 837
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 1 cut(s) 77
TaiI ACGT 2 cut(s) 35, 201
TaqI TCGA 1 cut(s) 638
TaqII GACCGA 1 cut(s) 25
TasI AATT 5 cut(s) 338, 412, 584, 735, 766
TatI WGTACW 4 cut(s) 545, 694, 762, 810
TfiI GAWTC 1 cut(s) 753
Tru1I TTAA 2 cut(s) 489, 865
Tru9I TTAA 2 cut(s) 489, 865
TscAI CASTG 1 cut(s) 315
TspDTI ATGAA 2 cut(s) 517, 601
TspGWI ACGGA 2 cut(s) 23, 85
TspRI CASTG 1 cut(s) 315
Vha464I CTTAAG 1 cut(s) 488
VpaK11BI GGWCC 1 cut(s) 10
XapI RAATTY 1 cut(s) 338
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.