MD00G1051800.v1.1

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004430
Physical Location & Seq
Forward (+)
9703375 .. 9705894
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1051800.v1.1.491

Sequence Viewer

Length: 921 bp
ATGCTAGCTCTCCAAGAAGCTCCCTCGCAATCCGGACTCCCAATTCCACTCACCCAGGCCCCAAATTCAAGCCTAGGTGACGGCGTTTCAGGATCCCGATTCTACAGCTGGCTCGGCGAGTGCCATGGGTTCTTCCACAATGTGGCTCTGATCGTGCCCTCCCTTCTCTTCGTCCTGTACTTGGCGTTTCAATCGAAAAAAAGTTTCTCGAAGCTCTCCCATGGCCGCTCTTATATCATGATTGCGTACTACGGGTGCCTCTGGCTTGTTAGCTTGCTCAACCTTGCTTGGTGCTGTTTTCAGGCATGGGAGTGCAGTCCTGGAAAACAACTAACATGGAATGTCTTATCTTTGTTCACAACCTCCGGGATGCTATTTTTGGAAATAAGCTTGCTGGCATTTTTGCTTCAAGGGAATTATGCAAGTGGGAGGGAAGCTTTGACACAGACTTTTGTCATCTCAGGGATCCTTGTTGTTTTGGATATACTTCTCAAGGCAATTTATCTATTTGGATTTGGCATTCCATTGTTCATTGACAATAATGACCATGCACATCGAACGAAGTGGGACTTGTGGGTTGTCCACAGGCTAGTGATTACTGCAATTTACGGCTTTATATTGTTCATGTACCATTCCAAGTGGAGAGAGAGGTTACCGGCAAGACCTGCATTCTACAAGTATACCGCTATCATGTTTACGTTAAACGTACTATCACTGTTTGCTTGCTTGCTTACTGGAAATGGGGCTGGATTTGGATTCTGGCTATATGGTGCTACTATTGTCTGTTATCACGCCTTTTATCTTCCGCTTTTGTATGTAACATTTCTGGCAGACTTCTTCCAGGAGGAAGATTTACATTTGGAGAATGTGTACTATTCAGAGATGAAAGATGCTGGTTTCTTTGACGCTGATTGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

307

Amino Acids

34.83

Weight (kDa)

6.12

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_40 PF10160 40 - 293 1.4e-122 Predicted membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 673
AccB1I GGYRCC 1 cut(s) 255
AccB7I CCANNNNNTGG 1 cut(s) 142
AccBSI CCGCTC 1 cut(s) 228
AccI GTMKAC 1 cut(s) 680
AccIII TCCGGA 1 cut(s) 32
AciI CCGC 3 cut(s) 226, 684, 806
AclWI GGATC 4 cut(s) 87, 100, 460, 473
AcoI YGGCCR 1 cut(s) 223
AcsI RAATTY 1 cut(s) 64
AdeI CACNNNGTG 1 cut(s) 142
AfaI GTAC 5 cut(s) 179, 248, 629, 708, 872
AfiI CCNNNNNNNGG 2 cut(s) 142, 181
AgsI TTSAA 3 cut(s) 69, 191, 410
AjnI CCWGG 3 cut(s) 54, 319, 840
AluBI AGCT 7 cut(s) 8, 20, 108, 214, 273, 390, 437
AluI AGCT 7 cut(s) 8, 20, 108, 214, 273, 390, 437
AlwI GGATC 4 cut(s) 87, 100, 460, 473
Aor13HI TCCGGA 1 cut(s) 32
AoxI GGCC 2 cut(s) 57, 223
ApoI RAATTY 1 cut(s) 64
AspA2I CCTAGG 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 58
AsuC2I CCSGG 1 cut(s) 367
AsuHPI GGTGA 2 cut(s) 43, 89
AsuNHI GCTAGC 1 cut(s) 4
AvrII CCTAGG 1 cut(s) 73
BaeGI GKGCMC 1 cut(s) 159
BamHI GGATCC 2 cut(s) 92, 465
BanI GGYRCC 1 cut(s) 255
BceAI ACGGC 2 cut(s) 97, 625
BciT130I CCWGG 3 cut(s) 56, 321, 842
BcnI CCSGG 1 cut(s) 367
BfaI CTAG 3 cut(s) 5, 74, 590
BfmI CTRYAG 1 cut(s) 103
BfuAI ACCTGC 1 cut(s) 673
BisI GCNGC 1 cut(s) 226
BlnI CCTAGG 1 cut(s) 73
BlsI GCNGC 1 cut(s) 227
Bme1390I CCNGG 4 cut(s) 56, 321, 367, 842
BmgT120I GGNCC 1 cut(s) 58
BmiI GGNNCC 4 cut(s) 60, 94, 257, 467
BmrFI CCNGG 4 cut(s) 56, 321, 367, 842
BmsI GCATC 2 cut(s) 360, 880
BmtI GCTAGC 1 cut(s) 8
BoxI GACNNNNGTC 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 367
BsaJI CCNNGG 4 cut(s) 54, 73, 124, 220
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 2 cut(s) 854, 884
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 181
Bse118I RCCGGY 1 cut(s) 655
Bse1I ACTGG 1 cut(s) 739
BseAI TCCGGA 1 cut(s) 32
BseBI CCWGG 3 cut(s) 56, 321, 842
BseDI CCNNGG 4 cut(s) 54, 73, 124, 220
BseGI GGATG 1 cut(s) 375
BseLI CCNNNNNNNGG 2 cut(s) 142, 181
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 1 cut(s) 739
BseSI GKGCMC 1 cut(s) 159
BsgI GTGCAG 1 cut(s) 334
BshFI GGCC 2 cut(s) 59, 225
BshNI GGYRCC 1 cut(s) 255
BsiSI CCGG 3 cut(s) 33, 366, 656
BslFI GGGAC 1 cut(s) 581
BslI CCNNNNNNNGG 2 cut(s) 142, 181
BsmFI GGGAC 1 cut(s) 581
BsmI GAATGC 2 cut(s) 519, 668
BsnI GGCC 2 cut(s) 59, 225
Bsp1286I GDGCHC 1 cut(s) 159
Bsp13I TCCGGA 1 cut(s) 32
Bsp143I GATC 3 cut(s) 92, 150, 465
Bsp19I CCATGG 2 cut(s) 124, 220
BspACI CCGC 3 cut(s) 226, 684, 806
BspANI GGCC 2 cut(s) 59, 225
BspCNI CTCAG 1 cut(s) 473
BspEI TCCGGA 1 cut(s) 32
BspHI TCATGA 1 cut(s) 237
BspLI GGNNCC 4 cut(s) 60, 94, 257, 467
BspMI ACCTGC 1 cut(s) 673
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 4 cut(s) 87, 100, 460, 473
BspT107I GGYRCC 1 cut(s) 255
BsrBI CCGCTC 1 cut(s) 228
BsrFI RCCGGY 1 cut(s) 655
BsrI ACTGG 1 cut(s) 739
BssAI RCCGGY 1 cut(s) 655
BssECI CCNNGG 4 cut(s) 54, 73, 124, 220
BssMI GATC 3 cut(s) 92, 150, 465
BssNAI GTATAC 1 cut(s) 681
BssT1I CCWWGG 3 cut(s) 73, 124, 220
Bst1107I GTATAC 1 cut(s) 681
Bst2UI CCWGG 3 cut(s) 56, 321, 842
Bst4CI ACNGT 1 cut(s) 717
Bst6I CTCTTC 1 cut(s) 173
BstAPI GCANNNNNTGC 1 cut(s) 665
BstC8I GCNNGC 7 cut(s) 6, 110, 275, 392, 396, 724, 728
BstDEI CTNAG 1 cut(s) 460
BstDSI CCRYGG 2 cut(s) 124, 220
BstEII GGTNACC 1 cut(s) 651
BstF5I GGATG 1 cut(s) 375
BstKTI GATC 3 cut(s) 95, 153, 468
BstMBI GATC 3 cut(s) 92, 150, 465
BstMWI GCNNNNNNNGC 2 cut(s) 114, 665
BstNI CCWGG 3 cut(s) 56, 321, 842
BstPAI GACNNNNGTC 1 cut(s) 452
BstPI GGTNACC 1 cut(s) 651
BstSCI CCNGG 4 cut(s) 54, 319, 365, 840
BstSFI CTRYAG 1 cut(s) 103
BstSLI GKGCMC 1 cut(s) 159
BstX2I RGATCY 2 cut(s) 92, 465
BstYI RGATCY 2 cut(s) 92, 465
BstZ17I GTATAC 1 cut(s) 681
BsuRI GGCC 2 cut(s) 59, 225
BtgI CCRYGG 2 cut(s) 124, 220
BtsCI GGATG 1 cut(s) 375
BtsIMutI CAGTG 1 cut(s) 713
BveI ACCTGC 1 cut(s) 673
Cac8I GCNNGC 7 cut(s) 6, 110, 275, 392, 396, 724, 728
CciI TCATGA 1 cut(s) 237
Cfr10I RCCGGY 1 cut(s) 655
Cfr13I GGNCC 1 cut(s) 58
CseI GACGC 1 cut(s) 914
Csp6I GTAC 5 cut(s) 178, 247, 628, 707, 871
CviAII CATG 8 cut(s) 125, 221, 238, 306, 336, 548, 625, 691
CviQI GTAC 5 cut(s) 178, 247, 628, 707, 871
DdeI CTNAG 1 cut(s) 460
DpnI GATC 3 cut(s) 94, 152, 467
DpnII GATC 3 cut(s) 92, 150, 465
DraIII CACNNNGTG 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
Eco130I CCWWGG 3 cut(s) 73, 124, 220
Eco91I GGTNACC 1 cut(s) 651
EcoO109I RGGNCCY 1 cut(s) 58
EcoO65I GGTNACC 1 cut(s) 651
EcoRII CCWGG 3 cut(s) 54, 319, 840
EcoT14I CCWWGG 3 cut(s) 73, 124, 220
ErhI CCWWGG 3 cut(s) 73, 124, 220
FaeI CATG 8 cut(s) 128, 224, 241, 309, 339, 551, 628, 694
FalI AAGNNNNNCTT 2 cut(s) 554, 586
FaqI GGGAC 1 cut(s) 581
FatI CATG 8 cut(s) 124, 220, 237, 305, 335, 547, 624, 690
FblI GTMKAC 1 cut(s) 680
Fnu4HI GCNGC 1 cut(s) 226
FokI GGATG 1 cut(s) 382
Fsp4HI GCNGC 1 cut(s) 226
FspBI CTAG 3 cut(s) 5, 74, 590
GluI GCNGC 1 cut(s) 226
HaeIII GGCC 2 cut(s) 59, 225
HapII CCGG 3 cut(s) 33, 366, 656
HgaI GACGC 1 cut(s) 914
Hin1II CATG 8 cut(s) 128, 224, 241, 309, 339, 551, 628, 694
HindIII AAGCTT 2 cut(s) 388, 435
HinfI GANTC 3 cut(s) 36, 99, 756
HpaII CCGG 3 cut(s) 33, 366, 656
HphI GGTGA 2 cut(s) 43, 89
Hpy166II GTNNAC 5 cut(s) 357, 583, 681, 696, 871
Hpy188I TCNGA 2 cut(s) 150, 880
Hpy188III TCNNGA 5 cut(s) 33, 90, 96, 208, 238
Hpy8I GTNNAC 5 cut(s) 357, 583, 681, 696, 871
HpyAV CCTTC 1 cut(s) 173
HpyCH4III ACNGT 1 cut(s) 717
HpyCH4IV ACGT 2 cut(s) 698, 705
HpyCH4V TGCA 5 cut(s) 315, 422, 551, 602, 668
HpyF10VI GCNNNNNNNGC 2 cut(s) 114, 665
HpyF3I CTNAG 1 cut(s) 460
HpySE526I ACGT 2 cut(s) 698, 705
Hsp92II CATG 8 cut(s) 128, 224, 241, 309, 339, 551, 628, 694
Kpn2I TCCGGA 1 cut(s) 32
Kzo9I GATC 3 cut(s) 92, 150, 465
LmnI GCTCC 1 cut(s) 25
LweI GCATC 2 cut(s) 360, 880
MaeI CTAG 3 cut(s) 5, 74, 590
MaeII ACGT 2 cut(s) 698, 705
MaeIII GTNAC 3 cut(s) 77, 651, 817
MalI GATC 3 cut(s) 94, 152, 467
MbiI CCGCTC 1 cut(s) 228
MboI GATC 3 cut(s) 92, 150, 465
MboII GAAGA 5 cut(s) 124, 160, 794, 829, 860
MflI RGATCY 2 cut(s) 92, 465
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 5 cut(s) 42, 64, 415, 498, 603
MlyI GAGTC 1 cut(s) 30
MnlI CCTC 7 cut(s) 34, 169, 269, 373, 423, 642, 838
MroI TCCGGA 1 cut(s) 32
MseI TTAA 1 cut(s) 701
MslI CAYNNNNRTG 1 cut(s) 310
MspA1I CMGCKG 1 cut(s) 108
MspI CCGG 3 cut(s) 33, 366, 656
MspR9I CCNGG 4 cut(s) 56, 321, 367, 842
Mva1269I GAATGC 2 cut(s) 519, 668
MvaI CCWGG 3 cut(s) 56, 321, 842
MwoI GCNNNNNNNGC 2 cut(s) 114, 665
NciI CCSGG 1 cut(s) 367
NcoI CCATGG 2 cut(s) 124, 220
NdeII GATC 3 cut(s) 92, 150, 465
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 8 cut(s) 128, 224, 241, 309, 339, 551, 628, 694
NlaIV GGNNCC 4 cut(s) 60, 94, 257, 467
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 1 cut(s) 77
PagI TCATGA 1 cut(s) 237
PctI GAATGC 2 cut(s) 519, 668
PfeI GAWTC 2 cut(s) 99, 756
PflMI CCANNNNNTGG 1 cut(s) 142
PfoI TCCNGGA 3 cut(s) 319, 365, 840
PkrI GCNGC 1 cut(s) 227
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
PshAI GACNNNNGTC 1 cut(s) 452
Psp6I CCWGG 3 cut(s) 54, 319, 840
PspEI GGTNACC 1 cut(s) 651
PspGI CCWGG 3 cut(s) 54, 319, 840
PspN4I GGNNCC 4 cut(s) 60, 94, 257, 467
PspPI GGNCC 1 cut(s) 58
PsuI RGATCY 2 cut(s) 92, 465
PvuII CAGCTG 1 cut(s) 108
RsaI GTAC 5 cut(s) 179, 248, 629, 708, 872
RsaNI GTAC 5 cut(s) 178, 247, 628, 707, 871
RseI CAYNNNNRTG 1 cut(s) 310
SaqAI TTAA 1 cut(s) 701
SatI GCNGC 1 cut(s) 226
Sau3AI GATC 3 cut(s) 92, 150, 465
Sau96I GGNCC 1 cut(s) 58
SchI GAGTC 1 cut(s) 30
ScrFI CCNGG 4 cut(s) 56, 321, 367, 842
SduI GDGCHC 1 cut(s) 159
SfaNI GCATC 2 cut(s) 360, 880
SfcI CTRYAG 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 310
SmlI CTYRAG 1 cut(s) 491
SmoI CTYRAG 1 cut(s) 491
Sse9I AATT 5 cut(s) 42, 64, 415, 498, 603
SsiI CCGC 3 cut(s) 226, 684, 806
SspMI CTAG 3 cut(s) 5, 74, 590
StyD4I CCNGG 4 cut(s) 54, 319, 365, 840
StyI CCWWGG 3 cut(s) 73, 124, 220
TaaI ACNGT 1 cut(s) 717
TaiI ACGT 2 cut(s) 701, 708
TaqI TCGA 3 cut(s) 194, 209, 556
TasI AATT 5 cut(s) 42, 64, 415, 498, 603
TatI WGTACW 2 cut(s) 177, 870
TauI GCSGC 1 cut(s) 228
TfiI GAWTC 2 cut(s) 99, 756
Tru1I TTAA 1 cut(s) 701
Tru9I TTAA 1 cut(s) 701
TscAI CASTG 1 cut(s) 720
TseFI GTSAC 1 cut(s) 77
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 3 cut(s) 520, 613, 899
TspRI CASTG 1 cut(s) 720
Van91I CCANNNNNTGG 1 cut(s) 142
XapI RAATTY 1 cut(s) 64
XmaJI CCTAGG 1 cut(s) 73
XmiI GTMKAC 1 cut(s) 680
XspI CTAG 3 cut(s) 5, 74, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.