FvH4_6g04200

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004430
Physical Location & Seq
Reverse (-)
2280358 .. 2282681
2324 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04200.t1

Sequence Viewer

Length: 912 bp
ATGGCAGCTCCGGTCCCGCACCCTCTGCTCCCAATTCCACTCCCGCAGGCCCCAAATTCAACCCTAGGCGAACCCATTTCGGGTCACAGATTGTACAGCTGGCTGGGTCAGTGCCATGGGCTCTGGCACAACGTGGCGCTCATCATGTCCTCGCTTCTCTTTGCTCTCTACTTGGGCTATCAATCCAGGAAGAGCTTTTCGAAGCTATCTCATGGCCGCTCGTATATCATGATGGCCTACTACGGCTGCCTCTGGCTCGTTAGCTTGCTCAATCTCGCTTGGTGCTTTCTTCAGGCATGGGAATGCACACCTGATAAAGAACTGGCATGGAATGTCTTATCTTTGTTTACAACTTCCGGAATGTTATTTTTGGAAGTAAGCTTGCTGGCATTTTTACTCCAAGGGAATTATGCAAGTGGACTGGAAGCGTTGACACGAACATTTGTTGTCTCAGGAATCCTTGTTGGTGTGGATATACTTCTGAAGGCAATTTATATGTTTGGATTTGGAATTCCGCTGTTTGTCGATGACAATGATCATACGCATCGGATGAAGTGGAACCTGTGGTCTGTCCATAGGCTAGTACTTACTGCAGTTTATGGTTTCATATTGTTCATGTACCATTCCAAGTGGAGAGACAGGTTACCTGCAAGACCTGCGTTCTACAACTATACAGCTATCATGTTTCTGATGAATGTGCTGGCATTGTTTGCTTGTGGACTCACGGGAAATGGAGCTGGATTTGCGTATTGGTTATATGGGGCCACTATTGTCTGTTACCATGCCTTCTATCTTCCTCTTTTATATGTAACATTTCTAGCAGACTTCTTCCAGGAGGACGATTTACATTTGGAGAATGTATATTATTCAGAGATGAAAGATGCTGGGTTCTTTGATGCTGATTGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

304

Amino Acids

34.61

Weight (kDa)

6.1

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_40 PF10160 37 - 290 9.7e-122 Predicted membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 655, 664
AccBSI CCGCTC 1 cut(s) 219
AccIII TCCGGA 1 cut(s) 356
AciI CCGC 4 cut(s) 17, 44, 217, 515
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 2 cut(s) 55, 510
AcuI CTGAAG 2 cut(s) 275, 503
AdeI CACNNNGTG 1 cut(s) 133
AfaI GTAC 3 cut(s) 95, 585, 620
AfiI CCNNNNNNNGG 1 cut(s) 80
AgsI TTSAA 1 cut(s) 60
AjnI CCWGG 2 cut(s) 185, 831
AluBI AGCT 8 cut(s) 8, 99, 195, 205, 264, 381, 677, 737
AluI AGCT 8 cut(s) 8, 99, 195, 205, 264, 381, 677, 737
Alw26I GTCTC 2 cut(s) 454, 630
Aor13HI TCCGGA 1 cut(s) 356
AoxI GGCC 4 cut(s) 48, 214, 234, 762
ApeKI GCWGC 2 cut(s) 5, 246
ApoI RAATTY 2 cut(s) 55, 510
AspA2I CCTAGG 1 cut(s) 64
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 3 cut(s) 13, 49, 762
AsuII TTCGAA 1 cut(s) 200
AvaII GGWCC 1 cut(s) 13
AvrII CCTAGG 1 cut(s) 64
BanII GRGCYC 1 cut(s) 123
BbvI GCAGC 2 cut(s) 17, 233
BccI CCATC 1 cut(s) 226
BceAI ACGGC 1 cut(s) 259
BciT130I CCWGG 2 cut(s) 187, 833
BclI TGATCA 1 cut(s) 535
BcoDI GTCTC 2 cut(s) 454, 630
BfaI CTAG 3 cut(s) 65, 581, 818
BfmI CTRYAG 1 cut(s) 591
BfoI RGCGCY 1 cut(s) 140
BfuAI ACCTGC 2 cut(s) 655, 664
BisI GCNGC 3 cut(s) 6, 217, 247
BlnI CCTAGG 1 cut(s) 64
BlsI GCNGC 3 cut(s) 7, 218, 248
BmcAI AGTACT 1 cut(s) 585
Bme1390I CCNGG 2 cut(s) 187, 833
Bme18I GGWCC 1 cut(s) 13
BmgT120I GGNCC 3 cut(s) 13, 49, 762
BmiI GGNNCC 4 cut(s) 15, 51, 560, 763
BmrFI CCNGG 2 cut(s) 187, 833
BmsI GCATC 3 cut(s) 553, 871, 886
Bpu14I TTCGAA 1 cut(s) 200
BsaJI CCNNGG 3 cut(s) 64, 115, 400
BsaWI WCCGGW 2 cut(s) 10, 356
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse1I ACTGG 2 cut(s) 327, 426
BseAI TCCGGA 1 cut(s) 356
BseBI CCWGG 2 cut(s) 187, 833
BseDI CCNNGG 3 cut(s) 64, 115, 400
BseGI GGATG 1 cut(s) 555
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMII CTCAG 1 cut(s) 465
BseNI ACTGG 2 cut(s) 327, 426
BseXI GCAGC 2 cut(s) 17, 233
BseYI CCCAGC 2 cut(s) 103, 884
BshFI GGCC 4 cut(s) 50, 216, 236, 764
BsiSI CCGG 2 cut(s) 11, 357
BslI CCNNNNNNNGG 1 cut(s) 80
BsmAI GTCTC 2 cut(s) 454, 630
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 4 cut(s) 50, 216, 236, 764
Bsp119I TTCGAA 1 cut(s) 200
Bsp1286I GDGCHC 1 cut(s) 123
Bsp13I TCCGGA 1 cut(s) 356
Bsp1407I TGTACA 1 cut(s) 93
Bsp143I GATC 1 cut(s) 535
Bsp19I CCATGG 1 cut(s) 115
BspACI CCGC 4 cut(s) 17, 44, 217, 515
BspANI GGCC 4 cut(s) 50, 216, 236, 764
BspCNI CTCAG 1 cut(s) 464
BspEI TCCGGA 1 cut(s) 356
BspHI TCATGA 1 cut(s) 228
BspLI GGNNCC 4 cut(s) 15, 51, 560, 763
BspMAI CTGCAG 1 cut(s) 595
BspMI ACCTGC 2 cut(s) 655, 664
BspQI GCTCTTC 1 cut(s) 185
BspT104I TTCGAA 1 cut(s) 200
BsrBI CCGCTC 1 cut(s) 219
BsrGI TGTACA 1 cut(s) 93
BsrI ACTGG 2 cut(s) 327, 426
BssECI CCNNGG 3 cut(s) 64, 115, 400
BssMI GATC 1 cut(s) 535
BssT1I CCWWGG 3 cut(s) 64, 115, 400
Bst2UI CCWGG 2 cut(s) 187, 833
Bst6I CTCTTC 1 cut(s) 185
BstAPI GCANNNNNTGC 3 cut(s) 25, 656, 710
BstAUI TGTACA 1 cut(s) 93
BstBI TTCGAA 1 cut(s) 200
BstC8I GCNNGC 6 cut(s) 48, 101, 266, 383, 387, 702
BstDEI CTNAG 1 cut(s) 451
BstDSI CCRYGG 1 cut(s) 115
BstEII GGTNACC 1 cut(s) 642
BstF5I GGATG 1 cut(s) 555
BstH2I RGCGCY 1 cut(s) 140
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 1 cut(s) 538
BstMAI GTCTC 2 cut(s) 454, 630
BstMBI GATC 1 cut(s) 535
BstMWI GCNNNNNNNGC 4 cut(s) 25, 656, 710, 743
BstNI CCWGG 2 cut(s) 187, 833
BstPI GGTNACC 1 cut(s) 642
BstSCI CCNGG 2 cut(s) 185, 831
BstSFI CTRYAG 1 cut(s) 591
BstV1I GCAGC 2 cut(s) 17, 233
BsuRI GGCC 4 cut(s) 50, 216, 236, 764
BtgI CCRYGG 1 cut(s) 115
BtsCI GGATG 1 cut(s) 555
BtsIMutI CAGTG 1 cut(s) 116
BveI ACCTGC 2 cut(s) 655, 664
Cac8I GCNNGC 6 cut(s) 48, 101, 266, 383, 387, 702
CciI TCATGA 1 cut(s) 228
CfoI GCGC 1 cut(s) 139
Cfr13I GGNCC 3 cut(s) 13, 49, 762
Csp6I GTAC 3 cut(s) 94, 584, 619
CviAII CATG 9 cut(s) 116, 145, 212, 229, 297, 327, 616, 682, 782
CviQI GTAC 3 cut(s) 94, 584, 619
DdeI CTNAG 1 cut(s) 451
DpnI GATC 1 cut(s) 537
DpnII GATC 1 cut(s) 535
DraIII CACNNNGTG 1 cut(s) 133
EaeI YGGCCR 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
Eco130I CCWWGG 3 cut(s) 64, 115, 400
Eco24I GRGCYC 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 13
Eco57I CTGAAG 2 cut(s) 275, 503
Eco91I GGTNACC 1 cut(s) 642
EcoO109I RGGNCCY 1 cut(s) 49
EcoO65I GGTNACC 1 cut(s) 642
EcoRI GAATTC 1 cut(s) 510
EcoRII CCWGG 2 cut(s) 185, 831
EcoT14I CCWWGG 3 cut(s) 64, 115, 400
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 3 cut(s) 64, 115, 400
FaeI CATG 9 cut(s) 119, 148, 215, 232, 300, 330, 619, 685, 785
FatI CATG 9 cut(s) 115, 144, 211, 228, 296, 326, 615, 681, 781
FauI CCCGC 2 cut(s) 24, 51
FbaI TGATCA 1 cut(s) 535
Fnu4HI GCNGC 3 cut(s) 6, 217, 247
FokI GGATG 1 cut(s) 562
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 3 cut(s) 6, 217, 247
FspBI CTAG 3 cut(s) 65, 581, 818
GlaI GCGC 1 cut(s) 138
GluI GCNGC 3 cut(s) 6, 217, 247
GsaI CCCAGC 2 cut(s) 107, 888
HaeII RGCGCY 1 cut(s) 140
HaeIII GGCC 4 cut(s) 50, 216, 236, 764
HapII CCGG 2 cut(s) 11, 357
HhaI GCGC 1 cut(s) 139
Hin1II CATG 9 cut(s) 119, 148, 215, 232, 300, 330, 619, 685, 785
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HincII GTYRAC 1 cut(s) 432
HindII GTYRAC 1 cut(s) 432
HindIII AAGCTT 1 cut(s) 379
HinfI GANTC 2 cut(s) 456, 720
HpaII CCGG 2 cut(s) 11, 357
Hpy166II GTNNAC 4 cut(s) 348, 419, 432, 719
Hpy188I TCNGA 4 cut(s) 483, 549, 690, 871
Hpy188III TCNNGA 3 cut(s) 229, 357, 453
Hpy8I GTNNAC 4 cut(s) 348, 419, 432, 719
HpyAV CCTTC 2 cut(s) 478, 796
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 4 cut(s) 306, 413, 593, 650
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 656, 710, 743
HpyF3I CTNAG 1 cut(s) 451
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 9 cut(s) 119, 148, 215, 232, 300, 330, 619, 685, 785
HspAI GCGC 1 cut(s) 137
Kpn2I TCCGGA 1 cut(s) 356
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 1 cut(s) 535
LguI GCTCTTC 1 cut(s) 185
LmnI GCTCC 3 cut(s) 13, 33, 734
Lsp1109I GCAGC 2 cut(s) 17, 233
LweI GCATC 3 cut(s) 553, 871, 886
MaeI CTAG 3 cut(s) 65, 581, 818
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 4 cut(s) 83, 642, 776, 808
MalI GATC 1 cut(s) 537
MbiI CCGCTC 1 cut(s) 219
MboI GATC 1 cut(s) 535
MboII GAAGA 4 cut(s) 202, 281, 785, 820
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 5 cut(s) 33, 55, 406, 489, 510
MlyI GAGTC 1 cut(s) 714
MnlI CCTC 5 cut(s) 33, 160, 260, 807, 829
MroI TCCGGA 1 cut(s) 356
MslI CAYNNNNRTG 1 cut(s) 301
MspA1I CMGCKG 2 cut(s) 99, 517
MspI CCGG 2 cut(s) 11, 357
MspR9I CCNGG 2 cut(s) 187, 833
Mva1269I GAATGC 1 cut(s) 308
MvaI CCWGG 2 cut(s) 187, 833
MwoI GCNNNNNNNGC 4 cut(s) 25, 656, 710, 743
NcoI CCATGG 1 cut(s) 115
NdeII GATC 1 cut(s) 535
NlaIII CATG 9 cut(s) 119, 148, 215, 232, 300, 330, 619, 685, 785
NlaIV GGNNCC 4 cut(s) 15, 51, 560, 763
NmuCI GTSAC 1 cut(s) 83
NspV TTCGAA 1 cut(s) 200
PagI TCATGA 1 cut(s) 228
PciSI GCTCTTC 1 cut(s) 185
PctI GAATGC 1 cut(s) 308
PfeI GAWTC 1 cut(s) 456
PfoI TCCNGGA 2 cut(s) 185, 831
PkrI GCNGC 3 cut(s) 7, 218, 248
PleI GAGTC 1 cut(s) 714
PpsI GAGTC 1 cut(s) 714
Psp6I CCWGG 2 cut(s) 185, 831
PspEI GGTNACC 1 cut(s) 642
PspFI CCCAGC 2 cut(s) 103, 884
PspGI CCWGG 2 cut(s) 185, 831
PspN4I GGNNCC 4 cut(s) 15, 51, 560, 763
PspPI GGNCC 3 cut(s) 13, 49, 762
PstI CTGCAG 1 cut(s) 595
PvuII CAGCTG 1 cut(s) 99
RsaI GTAC 3 cut(s) 95, 585, 620
RsaNI GTAC 3 cut(s) 94, 584, 619
RseI CAYNNNNRTG 1 cut(s) 301
SapI GCTCTTC 1 cut(s) 185
SatI GCNGC 3 cut(s) 6, 217, 247
Sau3AI GATC 1 cut(s) 535
Sau96I GGNCC 3 cut(s) 13, 49, 762
ScaI AGTACT 1 cut(s) 585
SchI GAGTC 1 cut(s) 714
ScrFI CCNGG 2 cut(s) 187, 833
SduI GDGCHC 1 cut(s) 123
SfaNI GCATC 3 cut(s) 553, 871, 886
SfcI CTRYAG 1 cut(s) 591
SfuI TTCGAA 1 cut(s) 200
SinI GGWCC 1 cut(s) 13
SmiMI CAYNNNNRTG 1 cut(s) 301
Sse9I AATT 5 cut(s) 33, 55, 406, 489, 510
SsiI CCGC 4 cut(s) 17, 44, 217, 515
SspMI CTAG 3 cut(s) 65, 581, 818
StyD4I CCNGG 2 cut(s) 185, 831
StyI CCWWGG 3 cut(s) 64, 115, 400
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 200, 525
TasI AATT 5 cut(s) 33, 55, 406, 489, 510
TatI WGTACW 2 cut(s) 93, 583
TauI GCSGC 1 cut(s) 219
TfiI GAWTC 1 cut(s) 456
TscAI CASTG 1 cut(s) 116
TseFI GTSAC 1 cut(s) 83
TseI GCWGC 2 cut(s) 5, 246
Tsp45I GTSAC 1 cut(s) 83
TspDTI ATGAA 5 cut(s) 566, 595, 604, 707, 890
TspRI CASTG 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 13
XapI RAATTY 2 cut(s) 55, 510
XmaJI CCTAGG 1 cut(s) 64
XspI CTAG 3 cut(s) 65, 581, 818
ZrmI AGTACT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.