RLG00000005094

Predicted membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004430
Physical Location & Seq
Reverse (-)
64975373 .. 64977410
2038 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005094

Sequence Viewer

Length: 909 bp
ATGGCAACAAATCTCCTACAACACCTGAACGCCTCGGTCAACGCGCCGAGGATCCCAAGCCTTCCCGCCACCGTCGACGGCGAGGATCGGAGGTTGTCCAGCTCAGACTGCCATGGTCTCTGGTACGACGCCGTTCTCGTCGTCCCTTCCGTCGTTTTCTTGGTCTACTTGGCCTTCCACGCCAAGAAGAACCTCAAGAAGCTCTCCAATGGCAGCTCTTATATCATGATTTCCTATTATGCCCTCGTCTGGGTCGCCGCCCTCCTCAACCTCGCCTGGTGCGCTCTTCAGGGCTGGCAATGCTCTGGTGGAAAGGCGGTGGCTTGGAATCTGTTGACTTTGTTCACAGCTTCTGCAATGATGTGTTTGGAGGTTAGCTTGGTGGCTTTTTTGCTCCAGGACAATTACTCAAGTGGAGTTGAAGCTCTAGCTCAGACTTTCCTTTTCTCAGGGATCGTTGTGAGCGTGGATTCACTGCTCAAGGCAATCTATGTATTCGGATTTGGGGTCCCTCTCTTCATGGTTGGTGTTGGAGGTACAACTGGAGTGAAATGGGGCCTATGGTTCTTCCACAAATCATTGCTTACTGCAGTCTATGGCTTCATATTGTTTGTGCATTTCTCCAAATGGAGAGAAAAGCTTCCGCCCAGACCAGCCTTCTACAACTACATTGTCGTGATGTTTGTATTTGGTGCTGTGACAATGTTTGCTTGTGGGCTTGCTGGAATCGGGGCTGGTTTTGGCATTTGGTTGTACAATTTGACAGAAATATGTTACCACGCTCTCTATCTTCCTTTTCTATATGTAACCTTCCTGGCAGATTTTTTCCAGGAAGAACAATTTCTTCTGGATAATGCATATTACTCTGAGATGAAGGATGCAGGGTTCTTTGATGCTGATTGGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_40

Protein Analysis

303

Amino Acids

33.65

Weight (kDa)

5.83

Isoelectric Point (pI)

34.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_40 PF10160 36 - 289 6.1e-109 Predicted membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 75, 165
AccII CGCG 1 cut(s) 44
AciI CCGC 4 cut(s) 66, 258, 317, 644
AclWI GGATC 4 cut(s) 46, 59, 93, 461
AcuI CTGAAG 1 cut(s) 272
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 3 cut(s) 125, 538, 755
AfiI CCNNNNNNNGG 2 cut(s) 249, 250
AgsI TTSAA 1 cut(s) 422
AjnI CCWGG 4 cut(s) 275, 396, 813, 828
AloI GAACNNNNNNTCC 2 cut(s) 869, 901
AluBI AGCT 8 cut(s) 102, 202, 216, 350, 378, 425, 431, 640
AluI AGCT 8 cut(s) 102, 202, 216, 350, 378, 425, 431, 640
Alw26I GTCTC 1 cut(s) 122
AlwI GGATC 4 cut(s) 46, 59, 93, 461
AlwNI CAGNNNCTG 1 cut(s) 353
AoxI GGCC 2 cut(s) 171, 556
ApeKI GCWGC 1 cut(s) 213
Asp700I GAANNNNTTC 2 cut(s) 639, 840
AspLEI GCGC 2 cut(s) 46, 284
AspS9I GGNCC 2 cut(s) 508, 556
AvaII GGWCC 1 cut(s) 508
BamHI GGATCC 1 cut(s) 51
BbvI GCAGC 1 cut(s) 225
BceAI ACGGC 2 cut(s) 94, 116
BciT130I CCWGG 4 cut(s) 277, 398, 815, 830
BcoDI GTCTC 1 cut(s) 122
BfaI CTAG 1 cut(s) 428
BfmI CTRYAG 1 cut(s) 588
BisI GCNGC 2 cut(s) 214, 258
BlsI GCNGC 2 cut(s) 215, 259
Bme1390I CCNGG 4 cut(s) 277, 398, 815, 830
Bme18I GGWCC 1 cut(s) 508
BmgT120I GGNCC 2 cut(s) 508, 556
BmiI GGNNCC 4 cut(s) 53, 509, 510, 557
BmrFI CCNGG 4 cut(s) 277, 398, 815, 830
BmsI GCATC 2 cut(s) 868, 883
BpmI CTGGAG 2 cut(s) 380, 564
BpuEI CTTGAG 3 cut(s) 179, 394, 464
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 3 cut(s) 33, 47, 112
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 250
Bse1I ACTGG 1 cut(s) 547
Bse3DI GCAATG 3 cut(s) 305, 363, 578
BseBI CCWGG 4 cut(s) 277, 398, 815, 830
BseDI CCNNGG 3 cut(s) 33, 47, 112
BseGI GGATG 1 cut(s) 883
BseLI CCNNNNNNNGG 2 cut(s) 249, 250
BseMI GCAATG 3 cut(s) 305, 363, 578
BseMII CTCAG 4 cut(s) 117, 446, 462, 858
BseNI ACTGG 1 cut(s) 547
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 1 cut(s) 225
Bsh1236I CGCG 1 cut(s) 44
BshFI GGCC 2 cut(s) 173, 558
BslFI GGGAC 2 cut(s) 128, 494
BslI CCNNNNNNNGG 2 cut(s) 249, 250
BsmAI GTCTC 1 cut(s) 122
BsmFI GGGAC 2 cut(s) 128, 494
BsnI GGCC 2 cut(s) 173, 558
Bso31I GGTCTC 1 cut(s) 122
Bsp1407I TGTACA 1 cut(s) 753
Bsp143I GATC 3 cut(s) 51, 85, 453
Bsp19I CCATGG 1 cut(s) 112
BspACI CCGC 4 cut(s) 66, 258, 317, 644
BspANI GGCC 2 cut(s) 173, 558
BspCNI CTCAG 4 cut(s) 116, 445, 461, 859
BspFNI CGCG 1 cut(s) 44
BspHI TCATGA 1 cut(s) 225
BspLI GGNNCC 4 cut(s) 53, 509, 510, 557
BspMAI CTGCAG 1 cut(s) 592
BspPI GGATC 4 cut(s) 46, 59, 93, 461
BspQI GCTCTTC 1 cut(s) 291
BspTNI GGTCTC 1 cut(s) 122
BsrDI GCAATG 3 cut(s) 305, 363, 578
BsrGI TGTACA 1 cut(s) 753
BsrI ACTGG 1 cut(s) 547
BssECI CCNNGG 3 cut(s) 33, 47, 112
BssMI GATC 3 cut(s) 51, 85, 453
BssNI GRCGYC 1 cut(s) 129
BssT1I CCWWGG 1 cut(s) 112
Bst2UI CCWGG 4 cut(s) 277, 398, 815, 830
Bst4CI ACNGT 1 cut(s) 73
Bst6I CTCTTC 2 cut(s) 291, 521
BstACI GRCGYC 1 cut(s) 129
BstAUI TGTACA 1 cut(s) 753
BstC8I GCNNGC 2 cut(s) 296, 720
BstDEI CTNAG 4 cut(s) 103, 432, 448, 867
BstDSI CCRYGG 1 cut(s) 112
BstF5I GGATG 1 cut(s) 883
BstFNI CGCG 1 cut(s) 44
BstHHI GCGC 2 cut(s) 46, 284
BstKTI GATC 3 cut(s) 54, 88, 456
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 3 cut(s) 51, 85, 453
BstMWI GCNNNNNNNGC 4 cut(s) 108, 179, 281, 300
BstNI CCWGG 4 cut(s) 277, 398, 815, 830
BstSCI CCNGG 4 cut(s) 275, 396, 813, 828
BstSFI CTRYAG 1 cut(s) 588
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 1 cut(s) 225
BstX2I RGATCY 1 cut(s) 51
BstYI RGATCY 1 cut(s) 51
BsuRI GGCC 2 cut(s) 173, 558
BtgI CCRYGG 1 cut(s) 112
BtsCI GGATG 1 cut(s) 883
BtsI GCAGTG 1 cut(s) 473
BtsIMutI CAGTG 1 cut(s) 473
Cac8I GCNNGC 2 cut(s) 296, 720
CaiI CAGNNNCTG 1 cut(s) 353
CciI TCATGA 1 cut(s) 225
CfoI GCGC 2 cut(s) 46, 284
Cfr13I GGNCC 2 cut(s) 508, 556
CseI GACGC 1 cut(s) 137
Csp6I GTAC 3 cut(s) 124, 537, 754
CviAII CATG 3 cut(s) 113, 226, 520
CviQI GTAC 3 cut(s) 124, 537, 754
DdeI CTNAG 4 cut(s) 103, 432, 448, 867
DpnI GATC 3 cut(s) 53, 87, 455
DpnII GATC 3 cut(s) 51, 85, 453
Eam1104I CTCTTC 2 cut(s) 291, 521
EarI CTCTTC 2 cut(s) 291, 521
EciI GGCGGA 1 cut(s) 633
Eco130I CCWWGG 1 cut(s) 112
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 508
Eco57I CTGAAG 1 cut(s) 272
EcoO109I RGGNCCY 2 cut(s) 508, 556
EcoRII CCWGG 4 cut(s) 275, 396, 813, 828
EcoT14I CCWWGG 1 cut(s) 112
EcoT22I ATGCAT 1 cut(s) 859
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 3 cut(s) 116, 229, 523
FaqI GGGAC 2 cut(s) 128, 494
FatI CATG 3 cut(s) 112, 225, 519
FauI CCCGC 1 cut(s) 73
FblI GTMKAC 2 cut(s) 75, 165
Fnu4HI GCNGC 2 cut(s) 214, 258
FokI GGATG 1 cut(s) 890
Fsp4HI GCNGC 2 cut(s) 214, 258
FspBI CTAG 1 cut(s) 428
GlaI GCGC 2 cut(s) 45, 283
GluI GCNGC 2 cut(s) 214, 258
GsuI CTGGAG 2 cut(s) 380, 564
HaeIII GGCC 2 cut(s) 173, 558
HgaI GACGC 1 cut(s) 137
HhaI GCGC 2 cut(s) 46, 284
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 3 cut(s) 116, 229, 523
Hin6I GCGC 2 cut(s) 44, 282
HinP1I GCGC 2 cut(s) 44, 282
HincII GTYRAC 3 cut(s) 40, 76, 336
HindII GTYRAC 3 cut(s) 40, 76, 336
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 3 cut(s) 328, 470, 726
Hpy166II GTNNAC 5 cut(s) 40, 76, 166, 336, 345
Hpy188I TCNGA 5 cut(s) 90, 106, 435, 500, 868
Hpy188III TCNNGA 4 cut(s) 196, 226, 676, 848
Hpy8I GTNNAC 5 cut(s) 40, 76, 166, 336, 345
Hpy99I CGWCG 5 cut(s) 77, 80, 131, 143, 155
HpyAV CCTTC 6 cut(s) 71, 156, 184, 667, 820, 868
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4V TGCA 5 cut(s) 356, 590, 616, 857, 881
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 179, 281, 300
HpyF3I CTNAG 4 cut(s) 103, 432, 448, 867
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 3 cut(s) 116, 229, 523
HspAI GCGC 2 cut(s) 44, 282
KflI GGGWCCC 1 cut(s) 508
Kzo9I GATC 3 cut(s) 51, 85, 453
LguI GCTCTTC 1 cut(s) 291
LmnI GCTCC 1 cut(s) 399
Lsp1109I GCAGC 1 cut(s) 225
LweI GCATC 2 cut(s) 868, 883
MaeI CTAG 1 cut(s) 428
MaeIII GTNAC 3 cut(s) 697, 773, 805
MalI GATC 3 cut(s) 53, 87, 455
MboI GATC 3 cut(s) 51, 85, 453
MboII GAAGA 7 cut(s) 199, 278, 508, 559, 782, 836, 845
MflI RGATCY 1 cut(s) 51
MluCI AATT 3 cut(s) 403, 757, 839
MmeI TCCRAC 1 cut(s) 511
Mph1103I ATGCAT 1 cut(s) 859
MroXI GAANNNNTTC 2 cut(s) 639, 840
MslI CAYNNNNRTG 1 cut(s) 674
MspR9I CCNGG 4 cut(s) 277, 398, 815, 830
MvaI CCWGG 4 cut(s) 277, 398, 815, 830
MvnI CGCG 1 cut(s) 44
MwoI GCNNNNNNNGC 4 cut(s) 108, 179, 281, 300
NcoI CCATGG 1 cut(s) 112
NdeII GATC 3 cut(s) 51, 85, 453
NlaIII CATG 3 cut(s) 116, 229, 523
NlaIV GGNNCC 4 cut(s) 53, 509, 510, 557
NmeAIII GCCGAG 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 697
NsiI ATGCAT 1 cut(s) 859
PagI TCATGA 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 291
PcsI WCGNNNNNNNCGW 3 cut(s) 135, 147, 462
PdmI GAANNNNTTC 2 cut(s) 639, 840
PfeI GAWTC 3 cut(s) 328, 470, 726
PfoI TCCNGGA 2 cut(s) 396, 828
PkrI GCNGC 2 cut(s) 215, 259
PpuMI RGGWCCY 1 cut(s) 508
Psp5II RGGWCCY 1 cut(s) 508
Psp6I CCWGG 4 cut(s) 275, 396, 813, 828
PspGI CCWGG 4 cut(s) 275, 396, 813, 828
PspN4I GGNNCC 4 cut(s) 53, 509, 510, 557
PspPI GGNCC 2 cut(s) 508, 556
PspPPI RGGWCCY 1 cut(s) 508
PstI CTGCAG 1 cut(s) 592
PstNI CAGNNNCTG 1 cut(s) 353
PsuI RGATCY 1 cut(s) 51
RsaI GTAC 3 cut(s) 125, 538, 755
RsaNI GTAC 3 cut(s) 124, 537, 754
RseI CAYNNNNRTG 1 cut(s) 674
SalI GTCGAC 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 291
SatI GCNGC 2 cut(s) 214, 258
Sau3AI GATC 3 cut(s) 51, 85, 453
Sau96I GGNCC 2 cut(s) 508, 556
ScrFI CCNGG 4 cut(s) 277, 398, 815, 830
SfaNI GCATC 2 cut(s) 868, 883
SfcI CTRYAG 1 cut(s) 588
SgrDI CGTCGACG 1 cut(s) 74
SinI GGWCC 1 cut(s) 508
SmiMI CAYNNNNRTG 1 cut(s) 674
SmlI CTYRAG 3 cut(s) 194, 409, 479
SmoI CTYRAG 3 cut(s) 194, 409, 479
Sse9I AATT 3 cut(s) 403, 757, 839
SsiI CCGC 4 cut(s) 66, 258, 317, 644
SspMI CTAG 1 cut(s) 428
StyD4I CCNGG 4 cut(s) 275, 396, 813, 828
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 73
TaqI TCGA 1 cut(s) 75
TaqII GACCGA 1 cut(s) 25
TasI AATT 3 cut(s) 403, 757, 839
TatI WGTACW 1 cut(s) 753
TauI GCSGC 1 cut(s) 260
TfiI GAWTC 3 cut(s) 328, 470, 726
TscAI CASTG 1 cut(s) 480
TseFI GTSAC 1 cut(s) 697
TseI GCWGC 1 cut(s) 213
Tsp45I GTSAC 1 cut(s) 697
TspDTI ATGAA 3 cut(s) 508, 592, 887
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 1 cut(s) 480
VpaK11BI GGWCC 1 cut(s) 508
XmiI GTMKAC 2 cut(s) 75, 165
XmnI GAANNNNTTC 2 cut(s) 639, 840
XspI CTAG 1 cut(s) 428
Zsp2I ATGCAT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.