Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006623

Physical Location & Seq

Genomic Coordinates Reverse (-)

3173299 .. 3179786

Gene Structure 6488 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G10270.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2193 bp

ATGGCTCTAGTTCAGAGACAACACTCTTATCTTCTCCGTTATTTTCGTCTCATGGCTTCTCGTCCTCGTCCTCGTCTCTTCTCTCACTCTCTATATCCTTCACTTCACCGTCACTCCTCAGCTCTCAGCTCTTCAACACCCAGGATAAAGTTTCAGTTAGCCAATGTTTTTAGTCAAAGACTTGTACAAAGAAATGCGGTCTCACCTAAAAGTTTTATGTCATCGACCATGGAGTCTCTCCAAGAGAGCTCTACCTCTAAGGATTATAGTTCCGAGCACATCCAAGTGTTGGAAGGCTTAGACCCTGTCAGGAAAAGACCTGGGATGTATATTGGTAGCACTGGATCTCGTGGTTTGCACCATCTGGTTTATGAGATCCTTGACAACGCAATTGACGAGGCCCAAGCTGGTTTTGCTTCAAAGATCGACGTTGTTTTACATTCAGATGATTCAGTCAGCATTTCAGACAATGGACGTGGGATACCCACAGATTTGCATCCTGCAACGGGAAAATCGTCTTTGGAAACTGTCCTTACGGTCTTACATGCAGGTGGTAAGTTTGGTGGCAAGAGTAGTGGGTACAGCGTGTCTGGTGGATTGCATGGCGTAGGTTTATCGGTTGTGAATGCATTGTCAGAGGCATTGGAGGTTATAGTTCGCAGAGATGGAATGGAGTTTCAGCAGAAGTATTCTCGTGGAAAGCCCGTAACAACACTTACATGTCATGTCCTACCACCAGAGTCAAGGGGGACTCAAGGGACATGCATCCGGTTCTGGCCTGACAAAGAAGTATTCACAACTGCAATTCAATTCGACCATAACACTATTGCTGGACGGATCAGGGAGCTTGCTTTTCTCAATCCAAAGGTTACAATCTCTCTAAAAAAGGAGGATGATGATCCAGAAAGGGACGTGTATAGTGAATATTTTTATGCTGGAGGGTTAACTGAATATGTTAGCTGGCTAAATACTGATAAGAAACCGCTTCATGATGTGCTGGGTTTCCGGAAAGAGATAAATGGCTCCACCGTTGACGTTTCACTTCAGTGGTGCTCAGATGCCTATTCAGACACGATGCTGGGATACGCGAATAGTATTCGGACCATAGATGGTGGGACACATATAGAAGGTGTGAAGGCTTCATTAACAAGAACTCTAAATTCCCTTGCAAAAAAGTTAAAGGTTATCAAGGAAAAGGATATTAGCTTAAGTGGGGAACATGTTAGGGAAGGATTGACCTGCATTGTCTCAGTCAAGGTTCCGAACCCTGAGTTTGAAGGTCAAACAAAGACAAGACTAGGAAATCCAGAGGTGAGAAAAATTGTTGACCAATCAGTCCAGGAGTACCTCACGGAGTACTTGGAATTGCATCCGGATGTACTTGAAAGCATTATTTCCAAATCCTTGAATGCGTACAAGGCTGCTCTGGCTGCCAAGAGGGCTAGGGAGTTGGTAAGATCAAAAAGCGTTTTGAAGTCATCCTCACTTCCTGGGAAACTTGCAGATTGCTCATCTACAGATCCTGCAGAATCTGAGATTTTCATTGTCGAAGGAGATTCGGCTGGTGGCAGCGCGAAACAGGGTCGTGACAGGCGTTTCCAGGCAATTCTTCCTTTGAGAGGCAAAATTTTGAATATTGAGAGAAAGGATGAAGCAGCAATGTATAAGAACGAAGAAATTCAAAATCTTATTCTTGGCCTTGGTCTTGGAGTTAAGGGAGAAGATTTTAACAAGGAGAATCTGCGTTACCATAAAATTATCATCTTAACAGATGCAGATGTTGATGGTGCACATATCCGGACACTTCTGTTGACCTTTTTCTTCAGATATCAGAGAGCCTTGTTTGACGCGGGTTGCATATACGTTGGTGTTCCACCTCTTTTCAAGGTTGAGAGGGGGAAACAGGCGCACTACTGCTACGATGATGCAGCTCTTAAAAAAATCACTGCTTCGTTCCCTGGAAACGCATCCTACAACATTCAGAGGTTCAAAGGTTTGGGTGAGATGATGCCTGCGCAGCTATGGGAAACAACGATGAATCCAGATACAAGGATATTGAAGCAACTAGTTGTTGACGACGCAGCAGAAACGAATATGGTCTTCTCATCTCTGATGGGTGCTCGGGTCGATGTCAGGAAGGAACTCATCAAAAGTGCTGCGACTAGGATGAATCTGGAGAATCTCGACATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003916 GO:0003918 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006265 GO:0006725 GO:0006807 GO:0006996 GO:0007059 GO:0008094 GO:0008150 GO:0008152 GO:0009295 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0017111 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0051276 GO:0061505 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_gyraseB DNA_gyraseB_C HATPase_c Toprim

Protein Analysis

730

Amino Acids

81.05

Weight (kDa)

8.64

Isoelectric Point (pI)

34.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HATPase_c	PF02518	126 - 272	7.8e-17	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
DNA_gyraseB	PF00204	324 - 493	2.2e-55	DNA gyrase B
Toprim	PF01751	522 - 622	3.6e-17	Toprim domain
DNA_gyraseB_C	PF00986	666 - 726	1.5e-25	DNA gyrase B subunit, carboxyl terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10270 AT3G10270 AT5G04130
fragaria_vesca	FvH4_6g25390 FvH4_6g25390 FvH4_6g25390
malus_domestica	MD12G1095800.v1.1 MD14G1090600.v1.1
prunus_persica	Prupe.7G033800_v2.0.a1
pyrus_communis	pycom12g08270
rosa_chinensis	RchiOBHm_Chr2g0125831
rosa_laevigata	RLG00000018881
rosa_multiflora	Rmu_co8103446.1_g000001 Rmu_sc0009079.1_g000002
rosa_roxburghii	Rroxscaffold_2G00117620 Rroxscaffold_2G00117680 Rroxscaffold_2G00125340 Rroxscaffold_2G00125370 Rroxscaffold_3G00238600 Rroxscaffold_4G00318520 Rroxscaffold_4G00324150 Rroxscaffold_5G00337460
rosa_rugosa	Rorug02G0259400
rosa_samantha	Rh2AG317700 Rh2BG326100
rosa_wichuraiana	Rw2G025700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	539
AasI	GACNNNNNNGTC	2 cut(s)	232, 1334
Acc16I	TGCGCA	1 cut(s)	2016
Acc36I	ACCTGC	2 cut(s)	539, 1247
AccB7I	CCANNNNNTGG	1 cut(s)	289
AccII	CGCG	3 cut(s)	1088, 1574, 1850
AccIII	TCCGGA	3 cut(s)	1005, 1372, 1797
AciI	CCGC	3 cut(s)	197, 983, 1850
AclWI	GGATC	5 cut(s)	352, 370, 845, 893, 1514
AcsI	RAATTY	3 cut(s)	1159, 1626, 1677
AcuI	CTGAAG	2 cut(s)	1028, 1807
AfaI	GTAC	6 cut(s)	186, 581, 1346, 1358, 1380, 1415
AfiI	CCNNNNNNNGG	3 cut(s)	289, 506, 1619
AflII	CTTAAG	1 cut(s)	1207
AflIII	ACRYGT	3 cut(s)	719, 912, 1219
AhlI	ACTAGT	1 cut(s)	2065
AjiI	CACGTC	2 cut(s)	476, 913
AjnI	CCWGG	6 cut(s)	140, 319, 1338, 1489, 1599, 1957
AleI	CACNNNNGTG	1 cut(s)	1045
AluBI	AGCT	9 cut(s)	122, 129, 249, 407, 847, 960, 1206, 1931, 2020
AluI	AGCT	9 cut(s)	122, 129, 249, 407, 847, 960, 1206, 1931, 2020
Alw21I	GWGCWC	5 cut(s)	251, 279, 1055, 1792, 2122
Alw26I	GTCTC	6 cut(s)	10, 53, 80, 205, 240, 1252
Alw44I	GTGCAC	1 cut(s)	1788
AlwI	GGATC	5 cut(s)	352, 370, 845, 893, 1514
AlwNI	CAGNNNCTG	2 cut(s)	1523, 1532
Ama87I	CYCGRG	1 cut(s)	2121
Aor13HI	TCCGGA	3 cut(s)	1005, 1372, 1797
AoxI	GGCC	3 cut(s)	399, 776, 1696
ApaLI	GTGCAC	1 cut(s)	1788
ApeKI	GCWGC	8 cut(s)	1421, 1430, 1569, 1655, 1928, 2017, 2081, 2156
ApoI	RAATTY	3 cut(s)	1159, 1626, 1677
Asp700I	GAANNNNTTC	1 cut(s)	1677
AspLEI	GCGC	3 cut(s)	1574, 1909, 2017
AspS9I	GGNCC	2 cut(s)	400, 1101
AsuHPI	GGTGA	4 cut(s)	98, 195, 1324, 2012
AvaI	CYCGRG	1 cut(s)	2121
AvaII	GGWCC	1 cut(s)	1101
BaeGI	GKGCMC	1 cut(s)	1792
BanII	GRGCYC	1 cut(s)	251
BauI	CACGAG	2 cut(s)	348, 693
BbsI	GAAGAC	1 cut(s)	2092
Bbv12I	GWGCWC	5 cut(s)	251, 279, 1055, 1792, 2122
BbvCI	CCTCAGC	1 cut(s)	118
BbvI	GCAGC	8 cut(s)	1408, 1417, 1581, 1667, 1940, 2029, 2093, 2143
BccI	CCATC	5 cut(s)	369, 659, 1103, 1778, 2107
BciT130I	CCWGG	6 cut(s)	142, 321, 1340, 1491, 1601, 1959
BciVI	GTATCC	2 cut(s)	474, 1076
BcoDI	GTCTC	6 cut(s)	10, 53, 80, 205, 240, 1252
BcuI	ACTAGT	1 cut(s)	2065
BfaI	CTAG	5 cut(s)	8, 1298, 1443, 2066, 2163
BfmI	CTRYAG	2 cut(s)	1515, 1524
BfrI	CTTAAG	1 cut(s)	1207
BfuAI	ACCTGC	2 cut(s)	539, 1247
BfuI	GTATCC	2 cut(s)	474, 1076
BglI	GCCNNNNNGGC	1 cut(s)	1439
BisI	GCNGC	8 cut(s)	1422, 1431, 1570, 1656, 1929, 2018, 2082, 2157
BlsI	GCNGC	8 cut(s)	1423, 1432, 1571, 1657, 1930, 2019, 2083, 2158
BmcAI	AGTACT	1 cut(s)	1358
Bme1390I	CCNGG	6 cut(s)	142, 321, 1340, 1491, 1601, 1959
Bme18I	GGWCC	1 cut(s)	1101
BmeT110I	CYCGRG	1 cut(s)	2121
BmgBI	CACGTC	2 cut(s)	476, 913
BmgT120I	GGNCC	2 cut(s)	400, 1101
BmiI	GGNNCC	2 cut(s)	1024, 1260
BmrFI	CCNGG	6 cut(s)	142, 321, 1340, 1491, 1601, 1959
BmsI	GCATC	9 cut(s)	505, 774, 1048, 1065, 1378, 1762, 1915, 1976, 1998
BpiI	GAAGAC	1 cut(s)	2092
BpmI	CTGGAG	1 cut(s)	957
Bpu10I	CCTNAGC	1 cut(s)	118
BpuEI	CTTGAG	1 cut(s)	738
BsaBI	GATNNNNATC	2 cut(s)	495, 897
BsaI	GGTCTC	1 cut(s)	205
BsaJI	CCNNGG	6 cut(s)	140, 228, 320, 1490, 1699, 1957
BsaWI	WCCGGW	4 cut(s)	768, 1005, 1372, 1797
BsaXI	ACNNNNNCTCC	2 cut(s)	98, 128
Bsc4I	CCNNNNNNNGG	3 cut(s)	289, 506, 1619
Bse1I	ACTGG	1 cut(s)	346
Bse3DI	GCAATG	1 cut(s)	1665
Bse8I	GATNNNNATC	2 cut(s)	495, 897
BseAI	TCCGGA	3 cut(s)	1005, 1372, 1797
BseBI	CCWGG	6 cut(s)	142, 321, 1340, 1491, 1601, 1959
BseDI	CCNNGG	6 cut(s)	140, 228, 320, 1490, 1699, 1957
BseJI	GATNNNNATC	2 cut(s)	495, 897
BseLI	CCNNNNNNNGG	3 cut(s)	289, 506, 1619
BseMI	GCAATG	1 cut(s)	1665
BseMII	CTCAG	6 cut(s)	132, 139, 1068, 1260, 1263, 1524
BseNI	ACTGG	1 cut(s)	346
BseRI	GAGGAG	1 cut(s)	106
BseSI	GKGCMC	1 cut(s)	1792
BseXI	GCAGC	8 cut(s)	1408, 1417, 1581, 1667, 1940, 2029, 2093, 2143
BseYI	CCCAGC	2 cut(s)	997, 1078
Bsh1236I	CGCG	3 cut(s)	1088, 1574, 1850
BshFI	GGCC	3 cut(s)	401, 778, 1698
BsiHKAI	GWGCWC	5 cut(s)	251, 279, 1055, 1792, 2122
BsiHKCI	CYCGRG	1 cut(s)	2121
BsiSI	CCGG	4 cut(s)	769, 1006, 1373, 1798
BslFI	GGGAC	4 cut(s)	763, 772, 923, 1129
BslI	CCNNNNNNNGG	3 cut(s)	289, 506, 1619
BsmAI	GTCTC	6 cut(s)	10, 53, 80, 205, 240, 1252
BsmBI	CGTCTC	2 cut(s)	53, 80
BsmFI	GGGAC	4 cut(s)	763, 772, 923, 1129
BsmI	GAATGC	2 cut(s)	631, 1414
BsnI	GGCC	3 cut(s)	401, 778, 1698
Bso31I	GGTCTC	1 cut(s)	205
BsoBI	CYCGRG	1 cut(s)	2121
Bsp1286I	GDGCHC	5 cut(s)	251, 279, 1055, 1792, 2122
Bsp13I	TCCGGA	3 cut(s)	1005, 1372, 1797
Bsp1407I	TGTACA	1 cut(s)	184
Bsp143I	GATC	7 cut(s)	344, 375, 423, 837, 898, 1457, 1519
Bsp19I	CCATGG	1 cut(s)	228
BspACI	CCGC	3 cut(s)	197, 983, 1850
BspANI	GGCC	3 cut(s)	401, 778, 1698
BspCNI	CTCAG	6 cut(s)	131, 138, 1067, 1261, 1262, 1525
BspEI	TCCGGA	3 cut(s)	1005, 1372, 1797
BspFNI	CGCG	3 cut(s)	1088, 1574, 1850
BspHI	TCATGA	1 cut(s)	988
BspLI	GGNNCC	2 cut(s)	1024, 1260
BspMAI	CTGCAG	1 cut(s)	1528
BspMI	ACCTGC	2 cut(s)	539, 1247
BspPI	GGATC	5 cut(s)	352, 370, 845, 893, 1514
BspQI	GCTCTTC	1 cut(s)	136
BspTI	CTTAAG	1 cut(s)	1207
BspTNI	GGTCTC	1 cut(s)	205
BsrDI	GCAATG	1 cut(s)	1665
BsrGI	TGTACA	1 cut(s)	184
BsrI	ACTGG	1 cut(s)	346
BssECI	CCNNGG	6 cut(s)	140, 228, 320, 1490, 1699, 1957
BssMI	GATC	7 cut(s)	344, 375, 423, 837, 898, 1457, 1519
BssSI	CACGAG	2 cut(s)	348, 693
BssT1I	CCWWGG	2 cut(s)	228, 1699
Bst2BI	CACGAG	2 cut(s)	348, 693
Bst2UI	CCWGG	6 cut(s)	142, 321, 1340, 1491, 1601, 1959
Bst4CI	ACNGT	4 cut(s)	110, 529, 538, 1030
Bst6I	CTCTTC	2 cut(s)	83, 136
BstAFI	CTTAAG	1 cut(s)	1207
BstAUI	TGTACA	1 cut(s)	184
BstC8I	GCNNGC	3 cut(s)	849, 962, 2013
BstDEI	CTNAG	8 cut(s)	118, 125, 258, 298, 1054, 1249, 1269, 1533
BstDSI	CCRYGG	1 cut(s)	228
BstENI	CCTNNNNNAGG	1 cut(s)	1617
BstFNI	CGCG	3 cut(s)	1088, 1574, 1850
BstHHI	GCGC	3 cut(s)	1574, 1909, 2017
BstKTI	GATC	7 cut(s)	347, 378, 426, 840, 901, 1460, 1522
BstMAI	GTCTC	6 cut(s)	10, 53, 80, 205, 240, 1252
BstMBI	GATC	7 cut(s)	344, 375, 423, 837, 898, 1457, 1519
BstMWI	GCNNNNNNNGC	6 cut(s)	413, 1418, 1427, 1430, 1439, 2017
BstNI	CCWGG	6 cut(s)	142, 321, 1340, 1491, 1601, 1959
BstNSI	RCATGY	4 cut(s)	548, 723, 765, 1223
BstSCI	CCNGG	6 cut(s)	140, 319, 1338, 1489, 1599, 1957
BstSFI	CTRYAG	2 cut(s)	1515, 1524
BstSLI	GKGCMC	1 cut(s)	1792
BstUI	CGCG	3 cut(s)	1088, 1574, 1850
BstV1I	GCAGC	8 cut(s)	1408, 1417, 1581, 1667, 1940, 2029, 2093, 2143
BstV2I	GAAGAC	1 cut(s)	2092
BstX2I	RGATCY	3 cut(s)	344, 375, 1519
BstYI	RGATCY	3 cut(s)	344, 375, 1519
BsuI	GTATCC	2 cut(s)	474, 1076
BsuRI	GGCC	3 cut(s)	401, 778, 1698
BtgI	CCRYGG	1 cut(s)	228
BtrI	CACGTC	2 cut(s)	476, 913
BtsI	GCAGTG	1 cut(s)	1944
BtsIMutI	CAGTG	3 cut(s)	339, 1052, 1944
BveI	ACCTGC	2 cut(s)	539, 1247
Cac8I	GCNNGC	3 cut(s)	849, 962, 2013
CaiI	CAGNNNCTG	2 cut(s)	1523, 1532
CciI	TCATGA	1 cut(s)	988
CfoI	GCGC	3 cut(s)	1574, 1909, 2017
Cfr13I	GGNCC	2 cut(s)	400, 1101
CseI	GACGC	2 cut(s)	1856, 2087
Csp6I	GTAC	6 cut(s)	185, 580, 1345, 1357, 1379, 1414
CspCI	CAANNNNNGTGG	4 cut(s)	457, 492, 556, 591
CviAII	CATG	9 cut(s)	52, 229, 545, 602, 720, 725, 762, 989, 1220
CviQI	GTAC	6 cut(s)	185, 580, 1345, 1357, 1379, 1414
DdeI	CTNAG	8 cut(s)	118, 125, 258, 298, 1054, 1249, 1269, 1533
DpnI	GATC	7 cut(s)	346, 377, 425, 839, 900, 1459, 1521
DpnII	GATC	7 cut(s)	344, 375, 423, 837, 898, 1457, 1519
DrdI	GACNNNNNNGTC	2 cut(s)	232, 1334
DseDI	GACNNNNNNGTC	2 cut(s)	232, 1334
Eam1104I	CTCTTC	2 cut(s)	83, 136
EarI	CTCTTC	2 cut(s)	83, 136
Ecl136II	GAGCTC	1 cut(s)	249
Eco130I	CCWWGG	2 cut(s)	228, 1699
Eco24I	GRGCYC	1 cut(s)	251
Eco31I	GGTCTC	1 cut(s)	205
Eco32I	GATATC	1 cut(s)	1829
Eco47I	GGWCC	1 cut(s)	1101
Eco53kI	GAGCTC	1 cut(s)	249
Eco57I	CTGAAG	2 cut(s)	1028, 1807
Eco88I	CYCGRG	1 cut(s)	2121
EcoICRI	GAGCTC	1 cut(s)	249
EcoNI	CCTNNNNNAGG	1 cut(s)	1617
EcoRII	CCWGG	6 cut(s)	140, 319, 1338, 1489, 1599, 1957
EcoRV	GATATC	1 cut(s)	1829
EcoT14I	CCWWGG	2 cut(s)	228, 1699
EcoT22I	ATGCAT	2 cut(s)	631, 767
EcoT38I	GRGCYC	1 cut(s)	251
ErhI	CCWWGG	2 cut(s)	228, 1699
Esp3I	CGTCTC	2 cut(s)	53, 80
FaeI	CATG	9 cut(s)	55, 232, 548, 605, 723, 728, 765, 992, 1223
FaqI	GGGAC	4 cut(s)	763, 772, 923, 1129
FatI	CATG	9 cut(s)	51, 228, 544, 601, 719, 724, 761, 988, 1219
FauI	CCCGC	1 cut(s)	1843
Fnu4HI	GCNGC	8 cut(s)	1422, 1431, 1570, 1656, 1929, 2018, 2082, 2157
FriOI	GRGCYC	1 cut(s)	251
Fsp4HI	GCNGC	8 cut(s)	1422, 1431, 1570, 1656, 1929, 2018, 2082, 2157
FspBI	CTAG	5 cut(s)	8, 1298, 1443, 2066, 2163
FspI	TGCGCA	1 cut(s)	2016
GlaI	GCGC	3 cut(s)	1573, 1908, 2016
GluI	GCNGC	8 cut(s)	1422, 1431, 1570, 1656, 1929, 2018, 2082, 2157
GsaI	CCCAGC	2 cut(s)	1001, 1082
GsuI	CTGGAG	1 cut(s)	957
HaeIII	GGCC	3 cut(s)	401, 778, 1698
HapII	CCGG	4 cut(s)	769, 1006, 1373, 1798
HgaI	GACGC	2 cut(s)	1856, 2087
HhaI	GCGC	3 cut(s)	1574, 1909, 2017
Hin1II	CATG	9 cut(s)	55, 232, 548, 605, 723, 728, 765, 992, 1223
Hin6I	GCGC	3 cut(s)	1572, 1907, 2015
HinP1I	GCGC	3 cut(s)	1572, 1907, 2015
HincII	GTYRAC	5 cut(s)	945, 1033, 1327, 1812, 2074
HindII	GTYRAC	5 cut(s)	945, 1033, 1327, 1812, 2074
HpaI	GTTAAC	1 cut(s)	945
HpaII	CCGG	4 cut(s)	769, 1006, 1373, 1798
HphI	GGTGA	4 cut(s)	98, 195, 1324, 2012
Hpy166II	GTNNAC	6 cut(s)	945, 1033, 1327, 1790, 1812, 2074
Hpy8I	GTNNAC	6 cut(s)	945, 1033, 1327, 1790, 1812, 2074
Hpy99I	CGWCG	2 cut(s)	431, 2081
HpyAV	CCTTC	8 cut(s)	108, 287, 1121, 1129, 1223, 1271, 1544, 2131
HpyCH4III	ACNGT	4 cut(s)	110, 529, 538, 1030
HpyCH4IV	ACGT	5 cut(s)	429, 475, 912, 1035, 1863
HpyF10VI	GCNNNNNNNGC	6 cut(s)	413, 1418, 1427, 1430, 1439, 2017
HpyF3I	CTNAG	8 cut(s)	118, 125, 258, 298, 1054, 1249, 1269, 1533
HpySE526I	ACGT	5 cut(s)	429, 475, 912, 1035, 1863
Hsp92II	CATG	9 cut(s)	55, 232, 548, 605, 723, 728, 765, 992, 1223
HspAI	GCGC	3 cut(s)	1572, 1907, 2015
Kpn2I	TCCGGA	3 cut(s)	1005, 1372, 1797
KspAI	GTTAAC	1 cut(s)	945
Kzo9I	GATC	7 cut(s)	344, 375, 423, 837, 898, 1457, 1519
LguI	GCTCTTC	1 cut(s)	136
LmnI	GCTCC	2 cut(s)	844, 1028
Lsp1109I	GCAGC	8 cut(s)	1408, 1417, 1581, 1667, 1940, 2029, 2093, 2143
LweI	GCATC	9 cut(s)	505, 774, 1048, 1065, 1378, 1762, 1915, 1976, 1998
MaeI	CTAG	5 cut(s)	8, 1298, 1443, 2066, 2163
MaeII	ACGT	5 cut(s)	429, 475, 912, 1035, 1863
MaeIII	GTNAC	5 cut(s)	110, 706, 868, 1586, 1745
MalI	GATC	7 cut(s)	346, 377, 425, 839, 900, 1459, 1521
MboI	GATC	7 cut(s)	344, 375, 423, 837, 898, 1457, 1519
MboII	GAAGA	8 cut(s)	23, 70, 123, 1601, 1685, 1733, 1813, 2092
MfeI	CAATTG	1 cut(s)	390
MflI	RGATCY	3 cut(s)	344, 375, 1519
MhlI	GDGCHC	5 cut(s)	251, 279, 1055, 1792, 2122
MlyI	GAGTC	3 cut(s)	242, 745, 749
MmeI	TCCRAC	1 cut(s)	270
Mph1103I	ATGCAT	2 cut(s)	631, 767
MroI	TCCGGA	3 cut(s)	1005, 1372, 1797
MroXI	GAANNNNTTC	1 cut(s)	1677
MseI	TTAA	8 cut(s)	944, 1145, 1178, 1208, 1713, 1728, 1766, 1935
MslI	CAYNNNNRTG	6 cut(s)	284, 444, 549, 718, 1045, 1374
MspCI	CTTAAG	1 cut(s)	1207
MspI	CCGG	4 cut(s)	769, 1006, 1373, 1798
MspR9I	CCNGG	6 cut(s)	142, 321, 1340, 1491, 1601, 1959
MunI	CAATTG	1 cut(s)	390
Mva1269I	GAATGC	2 cut(s)	631, 1414
MvaI	CCWGG	6 cut(s)	142, 321, 1340, 1491, 1601, 1959
MvnI	CGCG	3 cut(s)	1088, 1574, 1850
MwoI	GCNNNNNNNGC	6 cut(s)	413, 1418, 1427, 1430, 1439, 2017
NcoI	CCATGG	1 cut(s)	228
NdeII	GATC	7 cut(s)	344, 375, 423, 837, 898, 1457, 1519
NlaIII	CATG	9 cut(s)	55, 232, 548, 605, 723, 728, 765, 992, 1223
NlaIV	GGNNCC	2 cut(s)	1024, 1260
NmuCI	GTSAC	2 cut(s)	110, 1586
NsbI	TGCGCA	1 cut(s)	2016
NsiI	ATGCAT	2 cut(s)	631, 767
NspI	RCATGY	4 cut(s)	548, 723, 765, 1223
OliI	CACNNNNGTG	1 cut(s)	1045
PagI	TCATGA	1 cut(s)	988
PaqCI	CACCTGC	1 cut(s)	539
PciI	ACATGT	2 cut(s)	719, 1219
PciSI	GCTCTTC	1 cut(s)	136
PcsI	WCGNNNNNNNCGW	3 cut(s)	393, 512, 1591
PctI	GAATGC	2 cut(s)	631, 1414
PdmI	GAANNNNTTC	1 cut(s)	1677
PfeI	GAWTC	7 cut(s)	449, 1529, 1556, 1738, 2038, 2170, 2179
PflFI	GACNNNGTC	1 cut(s)	305
PflMI	CCANNNNNTGG	1 cut(s)	289
PfoI	TCCNGGA	1 cut(s)	1338
PkrI	GCNGC	8 cut(s)	1423, 1432, 1571, 1657, 1930, 2019, 2083, 2158
PleI	GAGTC	3 cut(s)	241, 745, 748
PpsI	GAGTC	3 cut(s)	241, 745, 748
PscI	ACATGT	2 cut(s)	719, 1219
Psp124BI	GAGCTC	1 cut(s)	251
Psp6I	CCWGG	6 cut(s)	140, 319, 1338, 1489, 1599, 1957
PspFI	CCCAGC	2 cut(s)	997, 1078
PspGI	CCWGG	6 cut(s)	140, 319, 1338, 1489, 1599, 1957
PspN4I	GGNNCC	2 cut(s)	1024, 1260
PspPI	GGNCC	2 cut(s)	400, 1101
PstI	CTGCAG	1 cut(s)	1528
PstNI	CAGNNNCTG	2 cut(s)	1523, 1532
PsuI	RGATCY	3 cut(s)	344, 375, 1519
PsyI	GACNNNGTC	1 cut(s)	305
RsaI	GTAC	6 cut(s)	186, 581, 1346, 1358, 1380, 1415
RsaNI	GTAC	6 cut(s)	185, 580, 1345, 1357, 1379, 1414
RseI	CAYNNNNRTG	6 cut(s)	284, 444, 549, 718, 1045, 1374
SacI	GAGCTC	1 cut(s)	251
SapI	GCTCTTC	1 cut(s)	136
SaqAI	TTAA	8 cut(s)	944, 1145, 1178, 1208, 1713, 1728, 1766, 1935
SatI	GCNGC	8 cut(s)	1422, 1431, 1570, 1656, 1929, 2018, 2082, 2157
Sau3AI	GATC	7 cut(s)	344, 375, 423, 837, 898, 1457, 1519
Sau96I	GGNCC	2 cut(s)	400, 1101
ScaI	AGTACT	1 cut(s)	1358
SchI	GAGTC	3 cut(s)	242, 745, 749
ScrFI	CCNGG	6 cut(s)	142, 321, 1340, 1491, 1601, 1959
SduI	GDGCHC	5 cut(s)	251, 279, 1055, 1792, 2122
SfaNI	GCATC	9 cut(s)	505, 774, 1048, 1065, 1378, 1762, 1915, 1976, 1998
SfcI	CTRYAG	2 cut(s)	1515, 1524
SinI	GGWCC	1 cut(s)	1101
SmiMI	CAYNNNNRTG	6 cut(s)	284, 444, 549, 718, 1045, 1374
SmlI	CTYRAG	2 cut(s)	753, 1207
SmoI	CTYRAG	2 cut(s)	753, 1207
SpeI	ACTAGT	1 cut(s)	2065
SsiI	CCGC	3 cut(s)	197, 983, 1850
SspI	AATATT	2 cut(s)	926, 1636
SspMI	CTAG	5 cut(s)	8, 1298, 1443, 2066, 2163
SstI	GAGCTC	1 cut(s)	251
StyD4I	CCNGG	6 cut(s)	140, 319, 1338, 1489, 1599, 1957
StyI	CCWWGG	2 cut(s)	228, 1699
TaaI	ACNGT	4 cut(s)	110, 529, 538, 1030
TaiI	ACGT	5 cut(s)	432, 478, 915, 1038, 1866
TaqI	TCGA	6 cut(s)	224, 426, 813, 1548, 2127, 2184
TatI	WGTACW	3 cut(s)	184, 1356, 1378
TfiI	GAWTC	7 cut(s)	449, 1529, 1556, 1738, 2038, 2170, 2179
Tru1I	TTAA	8 cut(s)	944, 1145, 1178, 1208, 1713, 1728, 1766, 1935
Tru9I	TTAA	8 cut(s)	944, 1145, 1178, 1208, 1713, 1728, 1766, 1935
TscAI	CASTG	3 cut(s)	346, 1052, 1951
TseFI	GTSAC	2 cut(s)	110, 1586
TseI	GCWGC	8 cut(s)	1421, 1430, 1569, 1655, 1928, 2017, 2081, 2156
Tsp45I	GTSAC	2 cut(s)	110, 1586
TspDTI	ATGAA	6 cut(s)	977, 1131, 1531, 1665, 2051, 2183
TspGWI	ACGGA	3 cut(s)	26, 850, 1367
TspRI	CASTG	3 cut(s)	346, 1052, 1951
Tth111I	GACNNNGTC	1 cut(s)	305
Van91I	CCANNNNNTGG	1 cut(s)	289
Vha464I	CTTAAG	1 cut(s)	1207
VneI	GTGCAC	1 cut(s)	1788
VpaK11BI	GGWCC	1 cut(s)	1101
XagI	CCTNNNNNAGG	1 cut(s)	1617
XapI	RAATTY	3 cut(s)	1159, 1626, 1677
XceI	RCATGY	4 cut(s)	548, 723, 765, 1223
XmnI	GAANNNNTTC	1 cut(s)	1677
XspI	CTAG	5 cut(s)	8, 1298, 1443, 2066, 2163
ZrmI	AGTACT	1 cut(s)	1358
Zsp2I	ATGCAT	2 cut(s)	631, 767

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.