Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0006623

Physical Location & Seq

Genomic Coordinates Forward (+)

19245755 .. 19259395

Gene Structure 13641 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g25390.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2175 bp

ATGGCGTTTCTTCTTCTTCGAGCTCATCCTCCTCCTACTCCTCCTCCTCCTTATCTTCGTTCCATGGCCTTGCGCTTCATCTCGCACTCTTGTCACTTGTCCACTTGTGCTCCTCGTCCCTCCCTCTTCTCCAGCAGCGCTCCCCCGCCGCCGCCACCACCCAGGATTGGGCTTCGATTGAAGGACGTTCCTTGCTGCCTCCGAAATGTGGTCTCCCCGAGAGCTTTCATGTCGGCAACCGAGGCTTATGGGGCGGATCAGATTCAGGTTCTAGAAGGCCTAGAGGCTGTTAGAAAGAGGCCCGGAATGTATATTGGGGACACCGGATTTCGCGGTCTTCATCACTTGGTCTATGAAATACTAGATAATGCTGTTGATGAGGCTCAAGCGGGATATGCTTCAAAGGTTGAGGTCATTTTACTAGCGGACAATTCTGTGAGCATCACAGACAATGGCCGTGGGATACCTACTGAAATGCACCCTGTAACCAAGAAATCTACATTGGAGACTGTGTTGACGGTTTTGCATGCCGGTGGAAAGTTTGGTGGTTCAAGTAGTGGCTATAGTGTGTCCGGTGGATTACATGGTGTGGGTTTATCCGTCGTTAATGCCTTATCTGAGGCACTAGAAGTTACTATTTGGCGTGATGGAATGGAATATCGGCAAAAGTATTCTCATGGAAAGCCTGTGACAACTCTCTCTTGTCATGTGCTTCCAGTTGAATATAAAGAAAGACAAGGAACACGAATCAGATTCTTGCCTGACAAACAAGTATTTAAAACTCTTGATCAGTTGGACTACCACACAATTGCTGGAAGAATTAGGGAGCTGGCATTTTTAAACCCTAATCTCATGATCACTCTAAGACAAGAGGATACGAATCCAGAGAAAGTCCAGCAAAATGAGTACTTCTATGTGGGGGGTTTGGTTGAATATGTTAAATGGCTAAATACTGATAAGAAAGCTCTTCATGAAGTGGTGGGTTTCAGAAAAGAAGTAGATGGAATCACTATTAATGTAGCTCTCCAGTGGTGTTCTGATGCATATTCAGATACAATGCTAGGGTATGCGAATAGCATACGTACTGCTGATGGAGGAACCCATATTGATGGACTGAAAGCTTCATTAACAAGAACACTGAATAACCTTGGGAAGAAGTCGAAGCTTATCAAGGATAAAGATATTACCTTAAATGGTGAGCATGTAAGAGAAGGACTGACATGTGTAGTTTCAGTCAAGGTTCCCAATCCAGAGTTTGAAGGCCAGACAAAGACTAGGTTGGGAAATCCGGAGGTGCGGAGAGTAGTTGATCAATCTCTTCAAGAGTATCTTACAGAGTACTTGGAGTTACATCCTGATGTACTGGATTCAATCCTTTCCAAGTCCTTAAATGCTCTCAAGGCAGCTCTTGCTGCAAAGAGGGCAAGGGAACTAGTCAGACAAAAGAGTGTTTTGCGTTCATCTTCTCTCCCAGGAAAGCTAGCTGATTGCTCATCAACCAATCCTGAAGAATCTGAAATCTTTATAGTTGAAGGAGATTCAGCTGGTGGAAGTGCAAAGCAAGGTCGCGATAGGCGCTTTCAGGCAATTCTTCCACTGAGGGGTAAAATCCTGAACATTGAAAGGAGGGACGAGGCAGCAATGTATAAAAATGAAGAGATTCAAAATCTGATTCTTGGACTTGGACTTGGGGTGAAGGGCGAGGACTTTAAAATGGAGGCTCTGCGTTACCATAAGATTATCATCTTAACAGATGCCGATGTGGATGGTGCTCATATCCGAACTCTGCTACTGACATTTTTCTTCAGATATCAGAGAGCCTTATTTGATGAGGGTTGCATATATGTTGGTGTTCCACCCTTTTACAAGGTCGAAAGGGGAAAGCAAGCATATTATTGCTATGATGATGCTGAACTTAAAAAGCTTCAGAGTTCCTTCCCTTCAAATGCATCGTATAATATACAACGGTTCAAAGGCTTGGGAGAAATGATGCCCACGCAATTATGGGAAACAACAATGGATCCTCAAAAAAGGCTGCTGAAACAATTAGGTGTGGATGATGCTGCTGAGGCTAACTTTGTCTTTTCCTCCCTTATGGGTTCTCGGGTGGATTTTCGGAAGGAGCTCATTCAAAATGCTGCACGCATGATCAACCTTGATCAGTTAGATATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003916 GO:0003918 GO:0005575 GO:0006139 GO:0006259 GO:0006265 GO:0006725 GO:0006807 GO:0006996 GO:0007059 GO:0008094 GO:0008150 GO:0008152 GO:0009295 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0017111 GO:0034641 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0051276 GO:0061505 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_gyraseB DNA_gyraseB_C HATPase_c Toprim

Protein Analysis

725

Amino Acids

80.71

Weight (kDa)

8.32

Isoelectric Point (pI)

41.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HATPase_c	PF02518	36 - 179	9.2e-18	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
DNA_gyraseB	PF00204	232 - 401	1.3e-55	DNA gyrase B
Toprim	PF01751	430 - 530	5.7e-18	Toprim domain
DNA_gyraseB_C	PF00986	574 - 635	1.1e-24	DNA gyrase B subunit, carboxyl terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10270 AT3G10270 AT5G04130
fragaria_vesca	FvH4_6g25390 FvH4_6g25390 FvH4_6g25390
malus_domestica	MD12G1095800.v1.1 MD14G1090600.v1.1
prunus_persica	Prupe.7G033800_v2.0.a1
pyrus_communis	pycom12g08270
rosa_chinensis	RchiOBHm_Chr2g0125831
rosa_laevigata	RLG00000018881
rosa_multiflora	Rmu_co8103446.1_g000001 Rmu_sc0009079.1_g000002
rosa_roxburghii	Rroxscaffold_2G00117620 Rroxscaffold_2G00117680 Rroxscaffold_2G00125340 Rroxscaffold_2G00125370 Rroxscaffold_3G00238600 Rroxscaffold_4G00318520 Rroxscaffold_4G00324150 Rroxscaffold_5G00337460
rosa_rugosa	Rorug02G0259400
rosa_samantha	Rh2AG317700 Rh2BG326100
rosa_wichuraiana	Rw2G025700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	333, 1569
AccIII	TCCGGA	1 cut(s)	1288
AciI	CCGC	8 cut(s)	146, 149, 152, 254, 333, 389, 425, 1297
AclWI	GGATC	3 cut(s)	264, 2015, 2028
AcoI	YGGCCR	1 cut(s)	454
AcuI	CTGAAG	3 cut(s)	1527, 1789, 1910
AfaI	GTAC	4 cut(s)	908, 1084, 1340, 1362
AfeI	AGCGCT	1 cut(s)	139
AfiI	CCNNNNNNNGG	4 cut(s)	167, 168, 208, 1601
AflIII	ACRYGT	1 cut(s)	1220
AhlI	ACTAGT	1 cut(s)	1432
AjnI	CCWGG	2 cut(s)	161, 1471
AjuI	GAANNNNNNNTTGG	2 cut(s)	485, 517
Alw21I	GWGCWC	4 cut(s)	25, 112, 1774, 2127
Alw26I	GTCTC	2 cut(s)	217, 500
AlwI	GGATC	3 cut(s)	264, 2015, 2028
Ama87I	CYCGRG	2 cut(s)	217, 2103
Aor13HI	TCCGGA	1 cut(s)	1288
Aor51HI	AGCGCT	1 cut(s)	139
AoxI	GGCC	5 cut(s)	66, 277, 299, 454, 1261
ApeKI	GCWGC	8 cut(s)	135, 195, 1403, 1412, 1637, 2035, 2063, 2138
AseI	ATTAAT	1 cut(s)	1014
Asp700I	GAANNNNTTC	1 cut(s)	1659
AspLEI	GCGC	3 cut(s)	75, 140, 1578
AspS9I	GGNCC	1 cut(s)	300
AsuC2I	CCSGG	1 cut(s)	303
AsuHPI	GGTGA	2 cut(s)	1208, 1705
AsuNHI	GCTAGC	1 cut(s)	1480
AvaI	CYCGRG	2 cut(s)	217, 2103
BamHI	GGATCC	1 cut(s)	2020
BanII	GRGCYC	2 cut(s)	25, 2127
BbsI	GAAGAC	1 cut(s)	329
Bbv12I	GWGCWC	4 cut(s)	25, 112, 1774, 2127
BbvCI	CCTCAGC	1 cut(s)	2067
BbvI	GCAGC	8 cut(s)	147, 182, 1399, 1415, 1649, 2022, 2050, 2125
BccI	CCATC	5 cut(s)	641, 995, 1085, 1103, 1760
BceAI	ACGGC	1 cut(s)	441
BciT130I	CCWGG	2 cut(s)	163, 1473
BciVI	GTATCC	2 cut(s)	456, 868
BclI	TGATCA	5 cut(s)	787, 855, 1309, 2148, 2158
BcnI	CCSGG	1 cut(s)	303
BcoDI	GTCTC	2 cut(s)	217, 500
BcuI	ACTAGT	1 cut(s)	1432
BfaI	CTAG	9 cut(s)	272, 281, 362, 422, 626, 1061, 1275, 1433, 1481
BfmI	CTRYAG	1 cut(s)	562
BfoI	RGCGCY	2 cut(s)	141, 1579
BfuI	GTATCC	2 cut(s)	456, 868
BmcAI	AGTACT	2 cut(s)	908, 1340
Bme1390I	CCNGG	3 cut(s)	163, 303, 1473
BmeT110I	CYCGRG	2 cut(s)	217, 2103
BmgT120I	GGNCC	1 cut(s)	300
BmiI	GGNNCC	3 cut(s)	1099, 1242, 2022
BmrFI	CCNGG	3 cut(s)	163, 303, 1473
BmsI	GCATC	7 cut(s)	450, 1030, 1744, 1897, 1958, 1980, 2050
BmtI	GCTAGC	1 cut(s)	1484
BpiI	GAAGAC	1 cut(s)	329
BpmI	CTGGAG	2 cut(s)	115, 1010
Bpu10I	CCTNAGC	1 cut(s)	2067
BpuEI	CTTGAG	2 cut(s)	369, 1382
BpuMI	CCSGG	1 cut(s)	303
BsaAI	YACGTR	1 cut(s)	1082
BsaBI	GATNNNNATC	2 cut(s)	879, 1742
BsaI	GGTCTC	1 cut(s)	217
BsaJI	CCNNGG	6 cut(s)	63, 161, 240, 457, 1147, 1471
BsaWI	WCCGGW	3 cut(s)	323, 572, 1288
BsaXI	ACNNNNNCTCC	6 cut(s)	28, 58, 94, 124, 497, 527
Bsc4I	CCNNNNNNNGG	4 cut(s)	167, 168, 208, 1601
Bse118I	RCCGGY	1 cut(s)	530
Bse1I	ACTGG	3 cut(s)	716, 1027, 1368
Bse3DI	GCAATG	1 cut(s)	1647
Bse8I	GATNNNNATC	2 cut(s)	879, 1742
BseAI	TCCGGA	1 cut(s)	1288
BseBI	CCWGG	2 cut(s)	163, 1473
BseDI	CCNNGG	6 cut(s)	63, 161, 240, 457, 1147, 1471
BseGI	GGATG	4 cut(s)	25, 1351, 1771, 2062
BseJI	GATNNNNATC	2 cut(s)	879, 1742
BseLI	CCNNNNNNNGG	4 cut(s)	167, 168, 208, 1601
BseMI	GCAATG	1 cut(s)	1647
BseMII	CTCAG	3 cut(s)	609, 1589, 2058
BseNI	ACTGG	3 cut(s)	716, 1027, 1368
BseRI	GAGGAG	5 cut(s)	21, 30, 33, 36, 102
BseXI	GCAGC	8 cut(s)	147, 182, 1399, 1415, 1649, 2022, 2050, 2125
BsgI	GTGCAG	1 cut(s)	2124
Bsh1236I	CGCG	2 cut(s)	333, 1569
BshFI	GGCC	5 cut(s)	68, 279, 301, 456, 1263
BsiHKAI	GWGCWC	4 cut(s)	25, 112, 1774, 2127
BsiHKCI	CYCGRG	2 cut(s)	217, 2103
BsiSI	CCGG	5 cut(s)	303, 324, 531, 573, 1289
BslFI	GGGAC	3 cut(s)	102, 332, 1643
BslI	CCNNNNNNNGG	4 cut(s)	167, 168, 208, 1601
BsmAI	GTCTC	2 cut(s)	217, 500
BsmFI	GGGAC	3 cut(s)	102, 332, 1643
BsnI	GGCC	5 cut(s)	68, 279, 301, 456, 1263
Bso31I	GGTCTC	1 cut(s)	217
BsoBI	CYCGRG	2 cut(s)	217, 2103
Bsp1286I	GDGCHC	4 cut(s)	25, 112, 1774, 2127
Bsp13I	TCCGGA	1 cut(s)	1288
Bsp143I	GATC	7 cut(s)	256, 787, 855, 1309, 2020, 2148, 2158
Bsp19I	CCATGG	1 cut(s)	63
Bsp68I	TCGCGA	1 cut(s)	1569
BspACI	CCGC	8 cut(s)	146, 149, 152, 254, 333, 389, 425, 1297
BspANI	GGCC	5 cut(s)	68, 279, 301, 456, 1263
BspCNI	CTCAG	3 cut(s)	610, 1590, 2059
BspEI	TCCGGA	1 cut(s)	1288
BspFNI	CGCG	2 cut(s)	333, 1569
BspHI	TCATGA	2 cut(s)	852, 970
BspLI	GGNNCC	3 cut(s)	1099, 1242, 2022
BspOI	GCTAGC	1 cut(s)	1484
BspPI	GGATC	3 cut(s)	264, 2015, 2028
BspQI	GCTCTTC	1 cut(s)	972
BspTNI	GGTCTC	1 cut(s)	217
BsrDI	GCAATG	1 cut(s)	1647
BsrFI	RCCGGY	1 cut(s)	530
BsrI	ACTGG	3 cut(s)	716, 1027, 1368
BssAI	RCCGGY	1 cut(s)	530
BssECI	CCNNGG	6 cut(s)	63, 161, 240, 457, 1147, 1471
BssMI	GATC	7 cut(s)	256, 787, 855, 1309, 2020, 2148, 2158
BssT1I	CCWWGG	2 cut(s)	63, 1147
Bst2UI	CCWGG	2 cut(s)	163, 1473
Bst4CI	ACNGT	3 cut(s)	511, 520, 1968
Bst6I	CTCTTC	4 cut(s)	131, 972, 1323, 1650
BstAPI	GCANNNNNTGC	1 cut(s)	1409
BstBAI	YACGTR	1 cut(s)	1082
BstC8I	GCNNGC	5 cut(s)	528, 831, 1482, 1887, 2143
BstDEI	CTNAG	4 cut(s)	618, 863, 1598, 2067
BstDSI	CCRYGG	2 cut(s)	63, 457
BstF5I	GGATG	4 cut(s)	25, 1351, 1771, 2062
BstFNI	CGCG	2 cut(s)	333, 1569
BstH2I	RGCGCY	2 cut(s)	141, 1579
BstHHI	GCGC	3 cut(s)	75, 140, 1578
BstKTI	GATC	7 cut(s)	259, 790, 858, 1312, 2023, 2151, 2161
BstMAI	GTCTC	2 cut(s)	217, 500
BstMBI	GATC	7 cut(s)	256, 787, 855, 1309, 2020, 2148, 2158
BstMWI	GCNNNNNNNGC	9 cut(s)	242, 251, 395, 1400, 1409, 1412, 1421, 1575, 2069
BstNI	CCWGG	2 cut(s)	163, 1473
BstNSI	RCATGY	3 cut(s)	530, 1205, 1224
BstSCI	CCNGG	3 cut(s)	161, 301, 1471
BstSFI	CTRYAG	1 cut(s)	562
BstSNI	TACGTA	1 cut(s)	1082
BstUI	CGCG	2 cut(s)	333, 1569
BstV1I	GCAGC	8 cut(s)	147, 182, 1399, 1415, 1649, 2022, 2050, 2125
BstV2I	GAAGAC	1 cut(s)	329
BstX2I	RGATCY	1 cut(s)	2020
BstXI	CCANNNNNNTGG	1 cut(s)	1109
BstYI	RGATCY	1 cut(s)	2020
BsuI	GTATCC	2 cut(s)	456, 868
BsuRI	GGCC	5 cut(s)	68, 279, 301, 456, 1263
BtgI	CCRYGG	2 cut(s)	63, 457
BtsCI	GGATG	4 cut(s)	25, 1351, 1771, 2062
BtsIMutI	CAGTG	3 cut(s)	1034, 1136, 1595
BtuMI	TCGCGA	1 cut(s)	1569
Cac8I	GCNNGC	5 cut(s)	528, 831, 1482, 1887, 2143
CciI	TCATGA	2 cut(s)	852, 970
CfoI	GCGC	3 cut(s)	75, 140, 1578
Cfr10I	RCCGGY	1 cut(s)	530
Cfr13I	GGNCC	1 cut(s)	300
Csp6I	GTAC	4 cut(s)	907, 1083, 1339, 1361
CspCI	CAANNNNNGTGG	2 cut(s)	439, 474
CviQI	GTAC	4 cut(s)	907, 1083, 1339, 1361
DdeI	CTNAG	4 cut(s)	618, 863, 1598, 2067
DpnI	GATC	7 cut(s)	258, 789, 857, 1311, 2022, 2150, 2160
DpnII	GATC	7 cut(s)	256, 787, 855, 1309, 2020, 2148, 2158
DraI	TTTAAA	3 cut(s)	778, 840, 1711
EaeI	YGGCCR	1 cut(s)	454
Eam1104I	CTCTTC	4 cut(s)	131, 972, 1323, 1650
EarI	CTCTTC	4 cut(s)	131, 972, 1323, 1650
EciI	GGCGGA	1 cut(s)	269
Ecl136II	GAGCTC	2 cut(s)	23, 2125
Eco105I	TACGTA	1 cut(s)	1082
Eco130I	CCWWGG	2 cut(s)	63, 1147
Eco147I	AGGCCT	1 cut(s)	279
Eco24I	GRGCYC	2 cut(s)	25, 2127
Eco31I	GGTCTC	1 cut(s)	217
Eco32I	GATATC	1 cut(s)	1811
Eco47III	AGCGCT	1 cut(s)	139
Eco53kI	GAGCTC	2 cut(s)	23, 2125
Eco57I	CTGAAG	3 cut(s)	1527, 1789, 1910
Eco88I	CYCGRG	2 cut(s)	217, 2103
EcoICRI	GAGCTC	2 cut(s)	23, 2125
EcoRII	CCWGG	2 cut(s)	161, 1471
EcoRV	GATATC	1 cut(s)	1811
EcoT14I	CCWWGG	2 cut(s)	63, 1147
EcoT22I	ATGCAT	2 cut(s)	1045, 1951
EcoT38I	GRGCYC	2 cut(s)	25, 2127
ErhI	CCWWGG	2 cut(s)	63, 1147
FalI	AAGNNNNNCTT	2 cut(s)	1314, 1346
FaqI	GGGAC	3 cut(s)	102, 332, 1643
FauI	CCCGC	2 cut(s)	153, 382
FbaI	TGATCA	5 cut(s)	787, 855, 1309, 2148, 2158
FokI	GGATG	4 cut(s)	12, 1338, 1778, 2069
FriOI	GRGCYC	2 cut(s)	25, 2127
FspBI	CTAG	9 cut(s)	272, 281, 362, 422, 626, 1061, 1275, 1433, 1481
GlaI	GCGC	3 cut(s)	74, 139, 1577
GsuI	CTGGAG	2 cut(s)	115, 1010
HaeII	RGCGCY	2 cut(s)	141, 1579
HaeIII	GGCC	5 cut(s)	68, 279, 301, 456, 1263
HapII	CCGG	5 cut(s)	303, 324, 531, 573, 1289
HhaI	GCGC	3 cut(s)	75, 140, 1578
Hin6I	GCGC	3 cut(s)	73, 138, 1576
HinP1I	GCGC	3 cut(s)	73, 138, 1576
HincII	GTYRAC	1 cut(s)	516
HindII	GTYRAC	1 cut(s)	516
HindIII	AAGCTT	3 cut(s)	1119, 1163, 1922
HpaII	CCGG	5 cut(s)	303, 324, 531, 573, 1289
HphI	GGTGA	2 cut(s)	1208, 1705
Hpy166II	GTNNAC	2 cut(s)	102, 516
Hpy8I	GTNNAC	2 cut(s)	102, 516
Hpy99I	CGWCG	1 cut(s)	605
HpyAV	CCTTC	9 cut(s)	175, 269, 1205, 1253, 1526, 1690, 1945, 1950, 2113
HpyCH4III	ACNGT	3 cut(s)	511, 520, 1968
HpyCH4IV	ACGT	2 cut(s)	186, 1081
HpyCH4V	TGCA	8 cut(s)	478, 526, 1043, 1415, 1556, 1839, 1949, 2141
HpyF10VI	GCNNNNNNNGC	9 cut(s)	242, 251, 395, 1400, 1409, 1412, 1421, 1575, 2069
HpyF3I	CTNAG	4 cut(s)	618, 863, 1598, 2067
HpySE526I	ACGT	2 cut(s)	186, 1081
HspAI	GCGC	3 cut(s)	73, 138, 1576
Kpn2I	TCCGGA	1 cut(s)	1288
Ksp22I	TGATCA	5 cut(s)	787, 855, 1309, 2148, 2158
Kzo9I	GATC	7 cut(s)	256, 787, 855, 1309, 2020, 2148, 2158
LguI	GCTCTTC	1 cut(s)	972
LmnI	GCTCC	4 cut(s)	115, 145, 826, 2122
Lsp1109I	GCAGC	8 cut(s)	147, 182, 1399, 1415, 1649, 2022, 2050, 2125
LweI	GCATC	7 cut(s)	450, 1030, 1744, 1897, 1958, 1980, 2050
MaeI	CTAG	9 cut(s)	272, 281, 362, 422, 626, 1061, 1275, 1433, 1481
MaeII	ACGT	2 cut(s)	186, 1081
MaeIII	GTNAC	6 cut(s)	92, 484, 631, 688, 1347, 1727
MalI	GATC	7 cut(s)	258, 789, 857, 1311, 2022, 2150, 2160
MboI	GATC	7 cut(s)	256, 787, 855, 1309, 2020, 2148, 2158
MfeI	CAATTG	1 cut(s)	807
MflI	RGATCY	1 cut(s)	2020
MhlI	GDGCHC	4 cut(s)	25, 112, 1774, 2127
MluCI	AATT	6 cut(s)	430, 807, 819, 1587, 2000, 2045
MmeI	TCCRAC	1 cut(s)	774
Mph1103I	ATGCAT	2 cut(s)	1045, 1951
MroI	TCCGGA	1 cut(s)	1288
MroXI	GAANNNNTTC	1 cut(s)	1659
MslI	CAYNNNNRTG	3 cut(s)	531, 1107, 1356
MspA1I	CMGCKG	1 cut(s)	1544
MspI	CCGG	5 cut(s)	303, 324, 531, 573, 1289
MspR9I	CCNGG	3 cut(s)	163, 303, 1473
MunI	CAATTG	1 cut(s)	807
MvaI	CCWGG	2 cut(s)	163, 1473
MvnI	CGCG	2 cut(s)	333, 1569
MwoI	GCNNNNNNNGC	9 cut(s)	242, 251, 395, 1400, 1409, 1412, 1421, 1575, 2069
NciI	CCSGG	1 cut(s)	303
NcoI	CCATGG	1 cut(s)	63
NdeII	GATC	7 cut(s)	256, 787, 855, 1309, 2020, 2148, 2158
NheI	GCTAGC	1 cut(s)	1480
NlaIV	GGNNCC	3 cut(s)	1099, 1242, 2022
NmuCI	GTSAC	2 cut(s)	92, 688
NruI	TCGCGA	1 cut(s)	1569
NsiI	ATGCAT	2 cut(s)	1045, 1951
NspI	RCATGY	3 cut(s)	530, 1205, 1224
PaeI	GCATGC	1 cut(s)	530
PagI	TCATGA	2 cut(s)	852, 970
PceI	AGGCCT	1 cut(s)	279
PciI	ACATGT	1 cut(s)	1220
PciSI	GCTCTTC	1 cut(s)	972
PdmI	GAANNNNTTC	1 cut(s)	1659
Ppu21I	YACGTR	1 cut(s)	1082
PscI	ACATGT	1 cut(s)	1220
PshBI	ATTAAT	1 cut(s)	1014
Psp124BI	GAGCTC	2 cut(s)	25, 2127
Psp6I	CCWGG	2 cut(s)	161, 1471
PspGI	CCWGG	2 cut(s)	161, 1471
PspN4I	GGNNCC	3 cut(s)	1099, 1242, 2022
PspPI	GGNCC	1 cut(s)	300
PsuI	RGATCY	1 cut(s)	2020
PvuII	CAGCTG	1 cut(s)	1544
RruI	TCGCGA	1 cut(s)	1569
RsaI	GTAC	4 cut(s)	908, 1084, 1340, 1362
RsaNI	GTAC	4 cut(s)	907, 1083, 1339, 1361
RseI	CAYNNNNRTG	3 cut(s)	531, 1107, 1356
SacI	GAGCTC	2 cut(s)	25, 2127
SapI	GCTCTTC	1 cut(s)	972
Sau3AI	GATC	7 cut(s)	256, 787, 855, 1309, 2020, 2148, 2158
Sau96I	GGNCC	1 cut(s)	300
ScaI	AGTACT	2 cut(s)	908, 1340
ScrFI	CCNGG	3 cut(s)	163, 303, 1473
SduI	GDGCHC	4 cut(s)	25, 112, 1774, 2127
SfaNI	GCATC	7 cut(s)	450, 1030, 1744, 1897, 1958, 1980, 2050
SfcI	CTRYAG	1 cut(s)	562
SmiMI	CAYNNNNRTG	3 cut(s)	531, 1107, 1356
SmlI	CTYRAG	2 cut(s)	384, 1397
SmoI	CTYRAG	2 cut(s)	384, 1397
SnaBI	TACGTA	1 cut(s)	1082
SpeI	ACTAGT	1 cut(s)	1432
SphI	GCATGC	1 cut(s)	530
Sse9I	AATT	6 cut(s)	430, 807, 819, 1587, 2000, 2045
SseBI	AGGCCT	1 cut(s)	279
SsiI	CCGC	8 cut(s)	146, 149, 152, 254, 333, 389, 425, 1297
SspMI	CTAG	9 cut(s)	272, 281, 362, 422, 626, 1061, 1275, 1433, 1481
SstI	GAGCTC	2 cut(s)	25, 2127
StuI	AGGCCT	1 cut(s)	279
StyD4I	CCNGG	3 cut(s)	161, 301, 1471
StyI	CCWWGG	2 cut(s)	63, 1147
TaaI	ACNGT	3 cut(s)	511, 520, 1968
TaiI	ACGT	2 cut(s)	189, 1084
TaqI	TCGA	4 cut(s)	19, 175, 1160, 1872
TasI	AATT	6 cut(s)	430, 807, 819, 1587, 2000, 2045
TatI	WGTACW	3 cut(s)	906, 1338, 1360
TauI	GCSGC	2 cut(s)	151, 154
TscAI	CASTG	3 cut(s)	1034, 1143, 1602
TseFI	GTSAC	2 cut(s)	92, 688
TseI	GCWGC	8 cut(s)	135, 195, 1403, 1412, 1637, 2035, 2063, 2138
Tsp45I	GTSAC	2 cut(s)	92, 688
TspDTI	ATGAA	9 cut(s)	67, 217, 329, 369, 959, 987, 1113, 1449, 1668
TspGWI	ACGGA	1 cut(s)	589
TspRI	CASTG	3 cut(s)	1034, 1143, 1602
VspI	ATTAAT	1 cut(s)	1014
XbaI	TCTAGA	1 cut(s)	271
XceI	RCATGY	3 cut(s)	530, 1205, 1224
XcmI	CCANNNNNNNNNTGG	1 cut(s)	809
XmnI	GAANNNNTTC	1 cut(s)	1659
XspI	CTAG	9 cut(s)	272, 281, 362, 422, 626, 1061, 1275, 1433, 1481
ZrmI	AGTACT	2 cut(s)	908, 1340
Zsp2I	ATGCAT	2 cut(s)	1045, 1951

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.