Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0006623

Physical Location & Seq

Genomic Coordinates Reverse (-)

48027064 .. 48040404

Gene Structure 13341 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00117680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGGCGCTTCTTCTTCTTCTGAGAGCTCATTCTCCTAGTCCTCCTTATCTTCTTCGCTTCATGGCCTTACGCTTCATCTCACACTCATGTCTCCACCCTCACTCTTCCTCCCTCTTCTTCAGCCCACGGCCACCGCCACCACCTCTTTGTGGTCACAGGATTGGGCTTCGATTGAAGGACGTTCCTAGCTGCTTTGCGGTTCGAAATGTGAGAGCTTTCATGTCGTCCAGCTCGGTAACCGAGGCTTTTCAAGGAAGCACTGCCTCCACGGCTTATGGCGCTGATCAGATTCAGGTTTTAGAAGGCTTAGAGGCCGTTAGAAAGAGGCCCGGAATGTATATTGGGGACACCGGATTTCGTGGTCTTCATCATCTGGTTTATGAAATACTAGACAATGCTGTTGATGAGGCTCAAGGCGGACATGCTTCCAAGGTTGAGGTCATTTTACTGGCCGACAATTCTGTCAGCATCACAGACAACGGCCGTGGGATACCTACTGATATGCATCCTGTAACCAAGAAATCTACATTAGAGACTGTGTTGACGGTTTTGCATGCTGGTGGCAAGTTTGGTGGTTCAAGTAGTGGCTATAGTGTGTCTGGTGGATTACATGGTGTGGGTTTATCAGTCGTTAATGCCTTGTCTGAGGCATTAGAAGTTACTATTTGGCGTGATGGAATGGAATACCAGCAAAAGTATTCTCGTGGAAAGCCTGTGACAACTCTCTCTTGTCATGTGCTTCCAATTGAATTTAAAGATAGACAAGGAACACGAATCAGATTCTTGCCTGACAAACAAGTATTTAAAACTCTTAATCAGTTCGACTACCACACAATTGCTGGAAGAATTAGGGAGCTAGCTTTTTTAAACCCTAATCTTATGATCACTCTTAGACAAGAGGATACGGATCCAGAGAAAATCCAGCACAATGAATACTTCTATGCAGGGGGTTTGGTTGAATATGTTAGATGGCTAAATACTGATAAGAAACCTCTTCATGAAGTGGTGGGTTTCAGAAAAGAAGTAGATGGAATCACTATTAATGTAGCTCTCCAGTGGTGTTCTGATGCATATTCAGATACAGTGCTAGGGTATGCTAATAGCATACGTACTGCTGACGGAGGAACCCATATTGATGGACTGAAAGCTTCGTTAACAAGAACGCTGAATAACCTTGGGAAGAAATCAAAGCTTATCAAGGATAAAGATATTACCTTAAGTGGTGAGCATGTAAGAGAAGGGCTGACATGTGTAGTTTCAGTCAAAGTTCCCAATCCAGAGTTCGAAGGCCAGACAAAGACTAGGTTGGGAAATCCAGAGGTGCGAAGAGTGGTTGATAATTCTCTTCAAGAGTATCTTACAGAATACTTGGAGTTACATCCTGATGTACTGGATTCAATCCTTTCCAAGTCCTTAAATGCTCTCAAGGCAGCTCTGGCTGCAAAGAGGGCAAGGGAACTAGTCAGACAAAAGAGTGTTTTGCGTTCGTCTTCTCTCCCAGGAAAGCTAGCTGATTGCTCATCAACCAATCCTGAAGAATCTGAAATCTTTATAGTTGAAGGAGATTCAGCTGGAGGAAGTGCAAAGCAAGGTCGTGATAGACGCTTTCAGGCAATTCTTCCACTAAGGGGTAAAATTCTGAACATTGAAAGGAGGGACGAGGCAGCGATGTATAAAAATGAAGAAATTCAAAATCTGATTCTTGGACTTGGACTTGGGGTGAAGGGAGAGGACTTTAAAAAGGAAGCTCTGCGTTACCATAAGATTATCATCTTAACTGATGCTGATGTAGATGGTGCTCACATCCGAACTCTGCTACTGACATTTTTCTTCAGATATCAGAGAGCCTTATTTGATGAGGGTTGCATATATGTTGGTGTTCCACCCTTTTACAAGGGGAAGCAAGCATATTATTGCTATGATGATGCTGAACTTAAAAAGCTTCAGAGTTCCTTCCCTTCAAATGCATCATACAGCATTCAAAGGTTCAAAGGACAACCTAGGGGTATCTGTGTTTTAGGACTTAGGGGTGGACTTAGGGTCGTAGTGTGTGGGGTGAAGGGGACTGTGATTAAAAGTCTGGGTTTTAAGGGAGAAGAAAGGGGAAATCTGGAATCACACCAGATTGGCCTGGGAGAAATGATGCCCACACAATTATGGGAAACAACAATGGATCCTCAGAAAAGGCTGCTGAAACAATTAAGTGTGGATGATGCTGCTGAGGCTAACTTTGTCTTTTCCTCCCTTATGGGTTCTCGGGTGGATTTCCGGAAGGAACTCATTCAAAATGCTGCACGCATGATCAACCTGGATCAGTTAGATATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003916 GO:0003918 GO:0005575 GO:0006139 GO:0006259 GO:0006265 GO:0006725 GO:0006807 GO:0006996 GO:0007059 GO:0008094 GO:0008150 GO:0008152 GO:0009295 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0017111 GO:0034641 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0051276 GO:0061505 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_gyraseB DNA_gyraseB_C HATPase_c Toprim

Protein Analysis

775

Amino Acids

86.0

Weight (kDa)

8.77

Isoelectric Point (pI)

39.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HATPase_c	PF02518	121 - 263	3e-17	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
DNA_gyraseB	PF00204	317 - 486	4e-55	DNA gyrase B
Toprim	PF01751	515 - 615	5.9e-18	Toprim domain
DNA_gyraseB_C	PF00986	710 - 762	9.7e-19	DNA gyrase B subunit, carboxyl terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10270 AT3G10270 AT5G04130
fragaria_vesca	FvH4_6g25390 FvH4_6g25390 FvH4_6g25390
malus_domestica	MD12G1095800.v1.1 MD14G1090600.v1.1
prunus_persica	Prupe.7G033800_v2.0.a1
pyrus_communis	pycom12g08270
rosa_chinensis	RchiOBHm_Chr2g0125831
rosa_laevigata	RLG00000018881
rosa_multiflora	Rmu_co8103446.1_g000001 Rmu_sc0009079.1_g000002
rosa_roxburghii	Rroxscaffold_2G00117620 Rroxscaffold_2G00117680 Rroxscaffold_2G00125340 Rroxscaffold_2G00125370 Rroxscaffold_3G00238600 Rroxscaffold_4G00318520 Rroxscaffold_4G00324150 Rroxscaffold_5G00337460
rosa_rugosa	Rorug02G0259400
rosa_samantha	Rh2AG317700 Rh2BG326100
rosa_wichuraiana	Rw2G025700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	461
AccIII	TCCGGA	1 cut(s)	2268
AciI	CCGC	3 cut(s)	134, 197, 417
AclWI	GGATC	5 cut(s)	902, 915, 2168, 2181, 2319
AcoI	YGGCCR	3 cut(s)	128, 450, 481
AcsI	RAATTY	3 cut(s)	749, 1635, 1686
AcuI	CTGAAG	4 cut(s)	103, 1554, 1816, 1928
AfaI	GTAC	2 cut(s)	1111, 1389
AfiI	CCNNNNNNNGG	2 cut(s)	149, 1628
AflII	CTTAAG	1 cut(s)	1216
AflIII	ACRYGT	1 cut(s)	1247
AhdI	GACNNNNNGTC	1 cut(s)	2039
AhlI	ACTAGT	1 cut(s)	1459
AjnI	CCWGG	3 cut(s)	1498, 2130, 2307
AjuI	GAANNNNNNNTTGG	2 cut(s)	1265, 1297
Alw21I	GWGCWC	2 cut(s)	28, 1801
Alw26I	GTCTC	2 cut(s)	95, 527
AlwI	GGATC	5 cut(s)	902, 915, 2168, 2181, 2319
Ama87I	CYCGRG	1 cut(s)	2256
Aor13HI	TCCGGA	1 cut(s)	2268
AoxI	GGCC	8 cut(s)	63, 128, 312, 326, 450, 481, 1288, 2128
ApeKI	GCWGC	7 cut(s)	189, 1430, 1439, 1664, 2188, 2216, 2291
ApoI	RAATTY	3 cut(s)	749, 1635, 1686
AseI	ATTAAT	1 cut(s)	1041
Asp700I	GAANNNNTTC	2 cut(s)	1686, 2280
AspA2I	CCTAGG	1 cut(s)	2000
AspLEI	GCGC	2 cut(s)	7, 281
AspS9I	GGNCC	1 cut(s)	327
AsuC2I	CCSGG	1 cut(s)	330
AsuHPI	GGTGA	3 cut(s)	1235, 1732, 2068
AsuII	TTCGAA	2 cut(s)	202, 1284
AsuNHI	GCTAGC	2 cut(s)	856, 1507
AvaI	CYCGRG	1 cut(s)	2256
AvrII	CCTAGG	1 cut(s)	2000
BamHI	GGATCC	2 cut(s)	907, 2173
BanII	GRGCYC	1 cut(s)	28
BauI	CACGAG	1 cut(s)	702
BbsI	GAAGAC	2 cut(s)	356, 1482
Bbv12I	GWGCWC	2 cut(s)	28, 1801
BbvCI	CCTCAGC	1 cut(s)	2220
BbvI	GCAGC	7 cut(s)	176, 1426, 1442, 1676, 2175, 2203, 2278
BccI	CCATC	5 cut(s)	668, 963, 1022, 1130, 1787
BceAI	ACGGC	5 cut(s)	143, 285, 299, 468, 496
BciT130I	CCWGG	3 cut(s)	1500, 2132, 2309
BciVI	GTATCC	2 cut(s)	483, 895
BclI	TGATCA	3 cut(s)	283, 882, 2301
BcnI	CCSGG	1 cut(s)	330
BcoDI	GTCTC	2 cut(s)	95, 527
BcuI	ACTAGT	1 cut(s)	1459
BfaI	CTAG	9 cut(s)	36, 186, 389, 857, 1088, 1302, 1460, 1508, 2001
BfmI	CTRYAG	1 cut(s)	589
BfoI	RGCGCY	2 cut(s)	8, 282
BfrI	CTTAAG	1 cut(s)	1216
BfuI	GTATCC	2 cut(s)	483, 895
BglI	GCCNNNNNGGC	1 cut(s)	269
BisI	GCNGC	7 cut(s)	190, 1431, 1440, 1665, 2189, 2217, 2292
BlnI	CCTAGG	1 cut(s)	2000
BlsI	GCNGC	7 cut(s)	191, 1432, 1441, 1666, 2190, 2218, 2293
Bme1390I	CCNGG	4 cut(s)	330, 1500, 2132, 2309
BmeRI	GACNNNNNGTC	1 cut(s)	2039
BmeT110I	CYCGRG	1 cut(s)	2256
BmgT120I	GGNCC	1 cut(s)	327
BmiI	GGNNCC	3 cut(s)	909, 1126, 2175
BmrFI	CCNGG	4 cut(s)	330, 1500, 2132, 2309
BmsI	GCATC	8 cut(s)	477, 514, 1057, 1771, 1915, 1976, 2133, 2203
BmtI	GCTAGC	2 cut(s)	860, 1511
BpiI	GAAGAC	2 cut(s)	356, 1482
BpmI	CTGGAG	2 cut(s)	1037, 1593
Bpu10I	CCTNAGC	1 cut(s)	2220
Bpu14I	TTCGAA	2 cut(s)	202, 1284
BpuEI	CTTGAG	2 cut(s)	396, 1409
BpuMI	CCSGG	1 cut(s)	330
BsaAI	YACGTR	1 cut(s)	1109
BsaBI	GATNNNNATC	3 cut(s)	504, 906, 1769
BsaJI	CCNNGG	9 cut(s)	125, 240, 267, 429, 484, 1174, 1498, 2000, 2131
BsaWI	WCCGGW	2 cut(s)	350, 2268
BsaXI	ACNNNNNCTCC	2 cut(s)	92, 122
Bsc4I	CCNNNNNNNGG	2 cut(s)	149, 1628
Bse1I	ACTGG	3 cut(s)	453, 1054, 1395
Bse8I	GATNNNNATC	3 cut(s)	504, 906, 1769
BseAI	TCCGGA	1 cut(s)	2268
BseBI	CCWGG	3 cut(s)	1500, 2132, 2309
BseDI	CCNNGG	9 cut(s)	125, 240, 267, 429, 484, 1174, 1498, 2000, 2131
BseGI	GGATG	4 cut(s)	505, 1378, 1803, 2215
BseJI	GATNNNNATC	3 cut(s)	504, 906, 1769
BseLI	CCNNNNNNNGG	2 cut(s)	149, 1628
BseMII	CTCAG	4 cut(s)	11, 636, 2192, 2211
BseNI	ACTGG	3 cut(s)	453, 1054, 1395
BseX3I	CGGCCG	1 cut(s)	481
BseXI	GCAGC	7 cut(s)	176, 1426, 1442, 1676, 2175, 2203, 2278
BsgI	GTGCAG	1 cut(s)	2277
Bsh1285I	CGRYCG	1 cut(s)	484
BshFI	GGCC	8 cut(s)	65, 130, 314, 328, 452, 483, 1290, 2130
BsiEI	CGRYCG	1 cut(s)	484
BsiHKAI	GWGCWC	2 cut(s)	28, 1801
BsiHKCI	CYCGRG	1 cut(s)	2256
BsiSI	CCGG	3 cut(s)	330, 351, 2269
BslFI	GGGAC	3 cut(s)	359, 1670, 2077
BslI	CCNNNNNNNGG	2 cut(s)	149, 1628
BsmAI	GTCTC	2 cut(s)	95, 527
BsmFI	GGGAC	3 cut(s)	359, 1670, 2077
BsmI	GAATGC	1 cut(s)	1977
BsnI	GGCC	8 cut(s)	65, 130, 314, 328, 452, 483, 1290, 2130
BsoBI	CYCGRG	1 cut(s)	2256
Bsp119I	TTCGAA	2 cut(s)	202, 1284
Bsp1286I	GDGCHC	2 cut(s)	28, 1801
Bsp13I	TCCGGA	1 cut(s)	2268
Bsp143I	GATC	6 cut(s)	283, 882, 907, 2173, 2301, 2311
BspACI	CCGC	3 cut(s)	134, 197, 417
BspANI	GGCC	8 cut(s)	65, 130, 314, 328, 452, 483, 1290, 2130
BspCNI	CTCAG	4 cut(s)	12, 637, 2191, 2212
BspEI	TCCGGA	1 cut(s)	2268
BspHI	TCATGA	1 cut(s)	997
BspLI	GGNNCC	3 cut(s)	909, 1126, 2175
BspOI	GCTAGC	2 cut(s)	860, 1511
BspPI	GGATC	5 cut(s)	902, 915, 2168, 2181, 2319
BspT104I	TTCGAA	2 cut(s)	202, 1284
BspTI	CTTAAG	1 cut(s)	1216
BsrI	ACTGG	3 cut(s)	453, 1054, 1395
BssECI	CCNNGG	9 cut(s)	125, 240, 267, 429, 484, 1174, 1498, 2000, 2131
BssMI	GATC	6 cut(s)	283, 882, 907, 2173, 2301, 2311
BssSI	CACGAG	1 cut(s)	702
BssT1I	CCWWGG	3 cut(s)	429, 1174, 2000
Bst2BI	CACGAG	1 cut(s)	702
Bst2UI	CCWGG	3 cut(s)	1500, 2132, 2309
Bst4CI	ACNGT	4 cut(s)	538, 547, 1084, 2068
Bst6I	CTCTTC	5 cut(s)	109, 119, 999, 1321, 1350
BstAFI	CTTAAG	1 cut(s)	1216
BstBAI	YACGTR	1 cut(s)	1109
BstBI	TTCGAA	2 cut(s)	202, 1284
BstC8I	GCNNGC	5 cut(s)	555, 858, 1509, 1905, 2296
BstDEI	CTNAG	9 cut(s)	20, 307, 645, 890, 1625, 2024, 2036, 2178, 2220
BstDSI	CCRYGG	3 cut(s)	125, 267, 484
BstEII	GGTNACC	1 cut(s)	235
BstF5I	GGATG	4 cut(s)	505, 1378, 1803, 2215
BstH2I	RGCGCY	2 cut(s)	8, 282
BstHHI	GCGC	2 cut(s)	7, 281
BstKTI	GATC	6 cut(s)	286, 885, 910, 2176, 2304, 2314
BstMAI	GTCTC	2 cut(s)	95, 527
BstMBI	GATC	6 cut(s)	283, 882, 907, 2173, 2301, 2311
BstMCI	CGRYCG	1 cut(s)	484
BstMWI	GCNNNNNNNGC	7 cut(s)	269, 278, 1427, 1436, 1439, 1448, 2222
BstNI	CCWGG	3 cut(s)	1500, 2132, 2309
BstNSI	RCATGY	4 cut(s)	425, 557, 1232, 1251
BstPI	GGTNACC	1 cut(s)	235
BstSCI	CCNGG	4 cut(s)	328, 1498, 2130, 2307
BstSFI	CTRYAG	1 cut(s)	589
BstSNI	TACGTA	1 cut(s)	1109
BstV1I	GCAGC	7 cut(s)	176, 1426, 1442, 1676, 2175, 2203, 2278
BstV2I	GAAGAC	2 cut(s)	356, 1482
BstX2I	RGATCY	2 cut(s)	907, 2173
BstXI	CCANNNNNNTGG	1 cut(s)	1136
BstYI	RGATCY	2 cut(s)	907, 2173
BstZI	CGGCCG	1 cut(s)	481
BsuI	GTATCC	2 cut(s)	483, 895
BsuRI	GGCC	8 cut(s)	65, 130, 314, 328, 452, 483, 1290, 2130
BtgI	CCRYGG	3 cut(s)	125, 267, 484
BtgZI	GCGATG	1 cut(s)	1682
BtsCI	GGATG	4 cut(s)	505, 1378, 1803, 2215
BtsI	GCAGTG	1 cut(s)	258
BtsIMutI	CAGTG	3 cut(s)	258, 1061, 1089
Cac8I	GCNNGC	5 cut(s)	555, 858, 1509, 1905, 2296
CciI	TCATGA	1 cut(s)	997
CfoI	GCGC	2 cut(s)	7, 281
Cfr13I	GGNCC	1 cut(s)	327
CseI	GACGC	1 cut(s)	1611
Csp6I	GTAC	2 cut(s)	1110, 1388
CspCI	CAANNNNNGTGG	4 cut(s)	466, 501, 553, 588
CviQI	GTAC	2 cut(s)	1110, 1388
DdeI	CTNAG	9 cut(s)	20, 307, 645, 890, 1625, 2024, 2036, 2178, 2220
DpnI	GATC	6 cut(s)	285, 884, 909, 2175, 2303, 2313
DpnII	GATC	6 cut(s)	283, 882, 907, 2173, 2301, 2311
DraI	TTTAAA	4 cut(s)	754, 805, 867, 1738
DrdI	GACNNNNNNGTC	1 cut(s)	461
DriI	GACNNNNNGTC	1 cut(s)	2039
DseDI	GACNNNNNNGTC	1 cut(s)	461
EaeI	YGGCCR	3 cut(s)	128, 450, 481
EagI	CGGCCG	1 cut(s)	481
Eam1104I	CTCTTC	5 cut(s)	109, 119, 999, 1321, 1350
Eam1105I	GACNNNNNGTC	1 cut(s)	2039
EarI	CTCTTC	5 cut(s)	109, 119, 999, 1321, 1350
EciI	GGCGGA	1 cut(s)	432
Ecl136II	GAGCTC	1 cut(s)	26
EclXI	CGGCCG	1 cut(s)	481
Eco105I	TACGTA	1 cut(s)	1109
Eco130I	CCWWGG	3 cut(s)	429, 1174, 2000
Eco24I	GRGCYC	1 cut(s)	28
Eco32I	GATATC	1 cut(s)	1838
Eco52I	CGGCCG	1 cut(s)	481
Eco53kI	GAGCTC	1 cut(s)	26
Eco57I	CTGAAG	4 cut(s)	103, 1554, 1816, 1928
Eco88I	CYCGRG	1 cut(s)	2256
Eco91I	GGTNACC	1 cut(s)	235
EcoICRI	GAGCTC	1 cut(s)	26
EcoO65I	GGTNACC	1 cut(s)	235
EcoRII	CCWGG	3 cut(s)	1498, 2130, 2307
EcoRV	GATATC	1 cut(s)	1838
EcoT14I	CCWWGG	3 cut(s)	429, 1174, 2000
EcoT22I	ATGCAT	3 cut(s)	507, 1072, 1969
EcoT38I	GRGCYC	1 cut(s)	28
ErhI	CCWWGG	3 cut(s)	429, 1174, 2000
FalI	AAGNNNNNCTT	2 cut(s)	1341, 1373
FaqI	GGGAC	3 cut(s)	359, 1670, 2077
FbaI	TGATCA	3 cut(s)	283, 882, 2301
Fnu4HI	GCNGC	7 cut(s)	190, 1431, 1440, 1665, 2189, 2217, 2292
FokI	GGATG	4 cut(s)	492, 1365, 1790, 2222
FriOI	GRGCYC	1 cut(s)	28
Fsp4HI	GCNGC	7 cut(s)	190, 1431, 1440, 1665, 2189, 2217, 2292
FspBI	CTAG	9 cut(s)	36, 186, 389, 857, 1088, 1302, 1460, 1508, 2001
GlaI	GCGC	2 cut(s)	6, 280
GluI	GCNGC	7 cut(s)	190, 1431, 1440, 1665, 2189, 2217, 2292
GsuI	CTGGAG	2 cut(s)	1037, 1593
HaeII	RGCGCY	2 cut(s)	8, 282
HaeIII	GGCC	8 cut(s)	65, 130, 314, 328, 452, 483, 1290, 2130
HapII	CCGG	3 cut(s)	330, 351, 2269
HgaI	GACGC	1 cut(s)	1611
HhaI	GCGC	2 cut(s)	7, 281
Hin6I	GCGC	2 cut(s)	5, 279
HinP1I	GCGC	2 cut(s)	5, 279
HincII	GTYRAC	2 cut(s)	543, 1155
HindII	GTYRAC	2 cut(s)	543, 1155
HindIII	AAGCTT	3 cut(s)	1146, 1190, 1940
HinfI	GANTC	9 cut(s)	289, 774, 780, 1032, 1394, 1538, 1565, 1699, 2114
HpaI	GTTAAC	1 cut(s)	1155
HpaII	CCGG	3 cut(s)	330, 351, 2269
HphI	GGTGA	3 cut(s)	1235, 1732, 2068
Hpy166II	GTNNAC	3 cut(s)	543, 1155, 2033
Hpy8I	GTNNAC	3 cut(s)	543, 1155, 2033
HpyCH4III	ACNGT	4 cut(s)	538, 547, 1084, 2068
HpyCH4IV	ACGT	2 cut(s)	180, 1108
HpyCH4V	TGCA	9 cut(s)	505, 553, 944, 1070, 1442, 1583, 1866, 1967, 2294
HpyF10VI	GCNNNNNNNGC	7 cut(s)	269, 278, 1427, 1436, 1439, 1448, 2222
HpyF3I	CTNAG	9 cut(s)	20, 307, 645, 890, 1625, 2024, 2036, 2178, 2220
HpySE526I	ACGT	2 cut(s)	180, 1108
HspAI	GCGC	2 cut(s)	5, 279
Kpn2I	TCCGGA	1 cut(s)	2268
Ksp22I	TGATCA	3 cut(s)	283, 882, 2301
KspAI	GTTAAC	1 cut(s)	1155
Kzo9I	GATC	6 cut(s)	283, 882, 907, 2173, 2301, 2311
LmnI	GCTCC	1 cut(s)	853
Lsp1109I	GCAGC	7 cut(s)	176, 1426, 1442, 1676, 2175, 2203, 2278
LweI	GCATC	8 cut(s)	477, 514, 1057, 1771, 1915, 1976, 2133, 2203
MaeI	CTAG	9 cut(s)	36, 186, 389, 857, 1088, 1302, 1460, 1508, 2001
MaeII	ACGT	2 cut(s)	180, 1108
MaeIII	GTNAC	7 cut(s)	152, 235, 511, 658, 715, 1374, 1754
MalI	GATC	6 cut(s)	285, 884, 909, 2175, 2303, 2313
MboI	GATC	6 cut(s)	283, 882, 907, 2173, 2301, 2311
MfeI	CAATTG	2 cut(s)	744, 834
MflI	RGATCY	2 cut(s)	907, 2173
MhlI	GDGCHC	2 cut(s)	28, 1801
Mph1103I	ATGCAT	3 cut(s)	507, 1072, 1969
MroI	TCCGGA	1 cut(s)	2268
MroXI	GAANNNNTTC	2 cut(s)	1686, 2280
MslI	CAYNNNNRTG	5 cut(s)	85, 558, 1134, 1383, 2155
MspA1I	CMGCKG	1 cut(s)	1571
MspCI	CTTAAG	1 cut(s)	1216
MspI	CCGG	3 cut(s)	330, 351, 2269
MspR9I	CCNGG	4 cut(s)	330, 1500, 2132, 2309
MunI	CAATTG	2 cut(s)	744, 834
Mva1269I	GAATGC	1 cut(s)	1977
MvaI	CCWGG	3 cut(s)	1500, 2132, 2309
MwoI	GCNNNNNNNGC	7 cut(s)	269, 278, 1427, 1436, 1439, 1448, 2222
NciI	CCSGG	1 cut(s)	330
NdeII	GATC	6 cut(s)	283, 882, 907, 2173, 2301, 2311
NheI	GCTAGC	2 cut(s)	856, 1507
NlaIV	GGNNCC	3 cut(s)	909, 1126, 2175
NmuCI	GTSAC	2 cut(s)	152, 715
NsiI	ATGCAT	3 cut(s)	507, 1072, 1969
NspI	RCATGY	4 cut(s)	425, 557, 1232, 1251
NspV	TTCGAA	2 cut(s)	202, 1284
PaeI	GCATGC	1 cut(s)	557
PagI	TCATGA	1 cut(s)	997
PciI	ACATGT	1 cut(s)	1247
PctI	GAATGC	1 cut(s)	1977
PdmI	GAANNNNTTC	2 cut(s)	1686, 2280
PfeI	GAWTC	9 cut(s)	289, 774, 780, 1032, 1394, 1538, 1565, 1699, 2114
PkrI	GCNGC	7 cut(s)	191, 1432, 1441, 1666, 2190, 2218, 2293
Ppu21I	YACGTR	1 cut(s)	1109
PscI	ACATGT	1 cut(s)	1247
PshBI	ATTAAT	1 cut(s)	1041
Psp124BI	GAGCTC	1 cut(s)	28
Psp6I	CCWGG	3 cut(s)	1498, 2130, 2307
PspEI	GGTNACC	1 cut(s)	235
PspGI	CCWGG	3 cut(s)	1498, 2130, 2307
PspN4I	GGNNCC	3 cut(s)	909, 1126, 2175
PspPI	GGNCC	1 cut(s)	327
PsuI	RGATCY	2 cut(s)	907, 2173
PvuII	CAGCTG	1 cut(s)	1571
RsaI	GTAC	2 cut(s)	1111, 1389
RsaNI	GTAC	2 cut(s)	1110, 1388
RseI	CAYNNNNRTG	5 cut(s)	85, 558, 1134, 1383, 2155
SacI	GAGCTC	1 cut(s)	28
SatI	GCNGC	7 cut(s)	190, 1431, 1440, 1665, 2189, 2217, 2292
Sau3AI	GATC	6 cut(s)	283, 882, 907, 2173, 2301, 2311
Sau96I	GGNCC	1 cut(s)	327
ScrFI	CCNGG	4 cut(s)	330, 1500, 2132, 2309
SduI	GDGCHC	2 cut(s)	28, 1801
SfaNI	GCATC	8 cut(s)	477, 514, 1057, 1771, 1915, 1976, 2133, 2203
SfcI	CTRYAG	1 cut(s)	589
SfuI	TTCGAA	2 cut(s)	202, 1284
SmiMI	CAYNNNNRTG	5 cut(s)	85, 558, 1134, 1383, 2155
SmlI	CTYRAG	3 cut(s)	411, 1216, 1424
SmoI	CTYRAG	3 cut(s)	411, 1216, 1424
SnaBI	TACGTA	1 cut(s)	1109
SpeI	ACTAGT	1 cut(s)	1459
SphI	GCATGC	1 cut(s)	557
SsiI	CCGC	3 cut(s)	134, 197, 417
SspMI	CTAG	9 cut(s)	36, 186, 389, 857, 1088, 1302, 1460, 1508, 2001
SstI	GAGCTC	1 cut(s)	28
StyD4I	CCNGG	4 cut(s)	328, 1498, 2130, 2307
StyI	CCWWGG	3 cut(s)	429, 1174, 2000
TaaI	ACNGT	4 cut(s)	538, 547, 1084, 2068
TaiI	ACGT	2 cut(s)	183, 1111
TaqI	TCGA	4 cut(s)	169, 202, 822, 1284
TatI	WGTACW	1 cut(s)	1387
TfiI	GAWTC	9 cut(s)	289, 774, 780, 1032, 1394, 1538, 1565, 1699, 2114
TscAI	CASTG	3 cut(s)	265, 1061, 1089
TseFI	GTSAC	2 cut(s)	152, 715
TseI	GCWGC	7 cut(s)	189, 1430, 1439, 1664, 2188, 2216, 2291
Tsp45I	GTSAC	2 cut(s)	152, 715
TspDTI	ATGAA	9 cut(s)	49, 64, 208, 356, 396, 945, 986, 1014, 1695
TspGWI	ACGGA	2 cut(s)	920, 1134
TspRI	CASTG	3 cut(s)	265, 1061, 1089
Vha464I	CTTAAG	1 cut(s)	1216
VspI	ATTAAT	1 cut(s)	1041
XapI	RAATTY	3 cut(s)	749, 1635, 1686
XceI	RCATGY	4 cut(s)	425, 557, 1232, 1251
XcmI	CCANNNNNNNNNTGG	1 cut(s)	836
XmaJI	CCTAGG	1 cut(s)	2000
XmnI	GAANNNNTTC	2 cut(s)	1686, 2280
XspI	CTAG	9 cut(s)	36, 186, 389, 857, 1088, 1302, 1460, 1508, 2001
Zsp2I	ATGCAT	3 cut(s)	507, 1072, 1969

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.