Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006623

Physical Location & Seq

Genomic Coordinates Forward (+)

13257122 .. 13274252

Gene Structure 17131 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1095800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2235 bp

ATGGCGCTTCTTCTTCTTCGACCTCCTCCTCCTCCTCCGCCCTCTTATCTTCGCTTCATGGCCTTACGCTTCCTCTCCCACTCATCTCTCCGCCCTCACTTCCCCTCTCCTTCTTCTTCTTCTTCTCCGTCTTCGTCTTCCTCGCTTGCTCGTCCCTCTCTCCTCTTCAACCCCCAACCCAGGGCGGAATTTCCATTGAAGGGCGTTGGTAGCTGCTGTGCGGTTCGAAATGTGGTTTCTGCAAGAGCTTTTATGTCCTCTACACCTGCAACCGAGGCTTTTCAAGGCACTACTGGCTCCAATGCTTATGGGTCCGATCAGATTCAGGTATTGGAAGGCTTAGACCCTGTTAGAAAGAGGCCAGGAATGTACATTGGGAGCACGGGACTTCGTGGTCTTCACCATTTGGTATATGAAATATTAGATAATGCAGTTGACGAGGCTCAAGCCGGATTTGCTTCAAAGGTTGAGGTCATTTTGCTAGCAGATGGTTCTGTGAGCATCACTGACAATGGTCGTGGGATTCCGACTGATATGCACCCAGCAACTAAGAAATCTTCCTTGGAGACTGTGTTGACGGTTTTACATGCCGGTGGCAAGTTTGGTGGTTCAAGTAGTGGTTATAGTGTGTCTGGTGGATTGCATGGTGTGGGGTTATCAGTCGTCAATGCCTTGTCTGAGGCGTTAGAAGTTACTGTTTGGCGTGATCGAATGGAATATCAGCAGAAGTATTCTCGTGGAAAACCTGTGACAACTCTATCCTGTCATGTGCTTCCTGTTGAATTCCAAGATCGGCAAGGAACACGCATCAGATTTTGGCCTGACAAAGAAGTCTTCACAACTAATATTCAGTTTGACTACAACACGATAGCTGGACGAATTAGGGAACTAGCTTTTCTAAACCCAAACCTCATGATCACTCTTAGGCAAGAGGATATCAATCCAGAGAAGAACCACCACAATGAATACTTTTATGCAGGGGGTTTGGTTGAATATGTAAAATGGCTAAATACTGATAAGAAACCGCTTCATGATGTGGTTGGCTTTAGAAAAGAAGTAGATGGAATCACTATTGATGTAGCTCTTCAGTGGTGTTCTGATGCATATTCAGATACAATGCTTGGGTATGCTAATAGCATACGAACTGTTGATGGAGGAACCCATATTGATGGAACGAAGGCTTCATTAACAAGAACTCTAAATAGTCTTGGAAAGAAGTCAAAACTTATCAAGGAAAAGGATATTACTTTGAGTGGTGAGCATGTGAGAGAAGGGTTGACGTGCGTCATCTCGGTCAAAGTTCCCAATCCGGAATTTGAAGGCCAAACAAAGACTAGGTTGGGAAATCCAGAGGTGCGGAGAGTGGTTGATCAATCACTTCAAGAGTATCTTACAGAATACTTGGAGTTGCATCCGGATGTACTGGATTCAATCCTTTCAAAGTCTTTGAATGCTCTCAAGGCAGCTCTGGCTGCAAAGAGGGCAAGAGAACTAGTCAGACAAAAAAGCGTGTTGAGATCATCTTCTCTGCCAGGAAAACTATCTGATTGCTCATCAACAAATCCTGAAGAATCAGAAATCTTTATAGTTGAAGGAGACTCAGCTGGTGGAAGTGCAAAACAGGGTCGTGATAGGCGCTTTCAGGCCATTCTGCCACTGAGGGGTAAAATTCTGAACATTGAAAGGAAGGATGAGGCAGCAATGTATAAAAATGAAGAGATTCAAAATCTAATTCTTGGTCTTGGACTTGGGGTGAAGGGTGAGGACTTTAAGAAGGAGGCTCTACGTTACCATAAGATTATCATTTTAACAGATGCTGATATAGATGGTGCTCACATCCGTACTCTGCTTCTGACATTTTTCTTCAGATATCAGAGAGCCTTATTTGATGAGGGCTACATATATGTTGGCGTTCCACCGCTTTACAAGGTTGAGAGGGGAAAGCAAGCCAAATATTGCTATGATGAAGCTGAACTTAAAATGCTTCAGAGCTCCTTCCCTTCGAATGCGTCTTACAACATTCAGAGGTTCAAAGGCCTGGGGGAAATGATGCCTGCCCAGTTATGGGAAACAACAATGAATCCTGAGCAGAGGCTTCTTAAACGATTAGGGGTTGAGGATGCTGCTGAGGCAAACATTGTGTTTTCATCCCTTATGGGTGCTCGGGTGGATTACCGGAAGGAGCTCATAAAGAATTCTGCGAGCATGATCGACCTCGATAAGTTGGATATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003916 GO:0003918 GO:0005575 GO:0006139 GO:0006259 GO:0006265 GO:0006725 GO:0006807 GO:0006996 GO:0007059 GO:0008094 GO:0008150 GO:0008152 GO:0009295 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0017111 GO:0034641 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0051276 GO:0061505 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_gyraseB DNA_gyraseB_C HATPase_c Toprim

Protein Analysis

745

Amino Acids

82.47

Weight (kDa)

8.4

Isoelectric Point (pI)

43.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HATPase_c	PF02518	131 - 276	3.3e-17	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
DNA_gyraseB	PF00204	328 - 497	7.6e-55	DNA gyrase B
Toprim	PF01751	526 - 627	1.4e-17	Toprim domain
DNA_gyraseB_C	PF00986	670 - 730	1.2e-24	DNA gyrase B subunit, carboxyl terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10270 AT3G10270 AT5G04130
fragaria_vesca	FvH4_6g25390 FvH4_6g25390 FvH4_6g25390
malus_domestica	MD12G1095800.v1.1 MD14G1090600.v1.1
prunus_persica	Prupe.7G033800_v2.0.a1
pyrus_communis	pycom12g08270
rosa_chinensis	RchiOBHm_Chr2g0125831
rosa_laevigata	RLG00000018881
rosa_multiflora	Rmu_co8103446.1_g000001 Rmu_sc0009079.1_g000002
rosa_roxburghii	Rroxscaffold_2G00117620 Rroxscaffold_2G00117680 Rroxscaffold_2G00125340 Rroxscaffold_2G00125370 Rroxscaffold_3G00238600 Rroxscaffold_4G00318520 Rroxscaffold_4G00324150 Rroxscaffold_5G00337460
rosa_rugosa	Rorug02G0259400
rosa_samantha	Rh2AG317700 Rh2BG326100
rosa_wichuraiana	Rw2G025700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	274
AasI	GACNNNNNNGTC	2 cut(s)	393, 830
Acc36I	ACCTGC	1 cut(s)	274
AccIII	TCCGGA	2 cut(s)	1309, 1414
AciI	CCGC	7 cut(s)	38, 91, 185, 221, 1025, 1357, 1919
AcsI	RAATTY	5 cut(s)	188, 782, 1313, 1668, 2194
AcuI	CTGAAG	4 cut(s)	1070, 1587, 1849, 1970
AfaI	GTAC	3 cut(s)	371, 1422, 1843
AfiI	CCNNNNNNNGG	6 cut(s)	180, 181, 200, 1661, 2064, 2065
AhlI	ACTAGT	1 cut(s)	1492
AjiI	CACGTC	1 cut(s)	1281
AjnI	CCWGG	4 cut(s)	179, 361, 1531, 2037
AjuI	GAANNNNNNNTTGG	2 cut(s)	545, 577
Alw21I	GWGCWC	5 cut(s)	383, 1834, 1994, 2164, 2187
Alw26I	GTCTC	2 cut(s)	560, 1590
AlwNI	CAGNNNCTG	1 cut(s)	1817
Ama87I	CYCGRG	1 cut(s)	2163
Aor13HI	TCCGGA	2 cut(s)	1309, 1414
AoxI	GGCC	6 cut(s)	60, 359, 818, 1321, 1644, 2035
ApeKI	GCWGC	5 cut(s)	213, 1463, 1472, 1697, 2123
ApoI	RAATTY	5 cut(s)	188, 782, 1313, 1668, 2194
Asp700I	GAANNNNTTC	1 cut(s)	1719
AspLEI	GCGC	2 cut(s)	7, 1638
AspS9I	GGNCC	1 cut(s)	312
AsuHPI	GGTGA	4 cut(s)	392, 1268, 1765, 1772
AsuII	TTCGAA	2 cut(s)	226, 2003
AsuNHI	GCTAGC	1 cut(s)	481
AvaI	CYCGRG	1 cut(s)	2163
AvaII	GGWCC	1 cut(s)	312
BanII	GRGCYC	2 cut(s)	1994, 2187
BauI	CACGAG	1 cut(s)	735
BbsI	GAAGAC	4 cut(s)	123, 129, 389, 826
Bbv12I	GWGCWC	5 cut(s)	383, 1834, 1994, 2164, 2187
BbvCI	CCTCAGC	1 cut(s)	2127
BbvI	GCAGC	5 cut(s)	200, 1459, 1475, 1709, 2110
BccI	CCATC	5 cut(s)	482, 1055, 1145, 1163, 1820
BcgI	CGANNNNNNTGC	2 cut(s)	517, 551
BciT130I	CCWGG	4 cut(s)	181, 363, 1533, 2039
BclI	TGATCA	2 cut(s)	915, 1369
BcoDI	GTCTC	2 cut(s)	560, 1590
BcuI	ACTAGT	1 cut(s)	1492
BfaI	CTAG	4 cut(s)	482, 890, 1335, 1493
BfoI	RGCGCY	2 cut(s)	8, 1639
BfuAI	ACCTGC	1 cut(s)	274
BisI	GCNGC	5 cut(s)	214, 1464, 1473, 1698, 2124
BlsI	GCNGC	5 cut(s)	215, 1465, 1474, 1699, 2125
Bme1390I	CCNGG	4 cut(s)	181, 363, 1533, 2039
Bme18I	GGWCC	1 cut(s)	312
BmeT110I	CYCGRG	1 cut(s)	2163
BmgBI	CACGTC	1 cut(s)	1281
BmgT120I	GGNCC	1 cut(s)	312
BmiI	GGNNCC	3 cut(s)	298, 313, 1159
BmrFI	CCNGG	4 cut(s)	181, 363, 1533, 2039
BmrI	ACTGGG	1 cut(s)	2053
BmsI	GCATC	7 cut(s)	510, 816, 1090, 1420, 1804, 2040, 2110
BmtI	GCTAGC	1 cut(s)	485
BmuI	ACTGGG	1 cut(s)	2053
BoxI	GACNNNNGTC	2 cut(s)	513, 1283
BpiI	GAAGAC	4 cut(s)	123, 129, 389, 826
Bpu10I	CCTNAGC	2 cut(s)	2085, 2127
Bpu14I	TTCGAA	2 cut(s)	226, 2003
BpuEI	CTTGAG	2 cut(s)	429, 1442
BsaBI	GATNNNNATC	1 cut(s)	939
BsaJI	CCNNGG	5 cut(s)	179, 180, 273, 561, 2038
BsaWI	WCCGGW	3 cut(s)	1309, 1414, 2175
BsaXI	ACNNNNNCTCC	2 cut(s)	557, 587
Bsc4I	CCNNNNNNNGG	6 cut(s)	180, 181, 200, 1661, 2064, 2065
Bse118I	RCCGGY	1 cut(s)	590
Bse1I	ACTGG	3 cut(s)	298, 1428, 2059
Bse3DI	GCAATG	1 cut(s)	1707
Bse8I	GATNNNNATC	1 cut(s)	939
BseAI	TCCGGA	2 cut(s)	1309, 1414
BseBI	CCWGG	4 cut(s)	181, 363, 1533, 2039
BseDI	CCNNGG	5 cut(s)	179, 180, 273, 561, 2038
BseGI	GGATG	6 cut(s)	1411, 1423, 1696, 1836, 2125, 2147
BseJI	GATNNNNATC	1 cut(s)	939
BseLI	CCNNNNNNNGG	6 cut(s)	180, 181, 200, 1661, 2064, 2065
BseMI	GCAATG	1 cut(s)	1707
BseMII	CTCAG	5 cut(s)	669, 1614, 1649, 2076, 2118
BseNI	ACTGG	3 cut(s)	298, 1428, 2059
BseRI	GAGGAG	5 cut(s)	15, 18, 21, 24, 152
BseXI	GCAGC	5 cut(s)	200, 1459, 1475, 1709, 2110
BseYI	CCCAGC	1 cut(s)	541
BshFI	GGCC	6 cut(s)	62, 361, 820, 1323, 1646, 2037
BsiHKAI	GWGCWC	5 cut(s)	383, 1834, 1994, 2164, 2187
BsiHKCI	CYCGRG	1 cut(s)	2163
BsiSI	CCGG	5 cut(s)	450, 591, 1310, 1415, 2176
BslFI	GGGAC	2 cut(s)	138, 399
BslI	CCNNNNNNNGG	6 cut(s)	180, 181, 200, 1661, 2064, 2065
BsmAI	GTCTC	2 cut(s)	560, 1590
BsmFI	GGGAC	2 cut(s)	138, 399
BsmI	GAATGC	2 cut(s)	1456, 2011
BsnI	GGCC	6 cut(s)	62, 361, 820, 1323, 1646, 2037
BsoBI	CYCGRG	1 cut(s)	2163
Bsp119I	TTCGAA	2 cut(s)	226, 2003
Bsp1286I	GDGCHC	5 cut(s)	383, 1834, 1994, 2164, 2187
Bsp13I	TCCGGA	2 cut(s)	1309, 1414
Bsp1407I	TGTACA	1 cut(s)	369
Bsp143I	GATC	7 cut(s)	316, 706, 790, 915, 1369, 1517, 2208
BspACI	CCGC	7 cut(s)	38, 91, 185, 221, 1025, 1357, 1919
BspANI	GGCC	6 cut(s)	62, 361, 820, 1323, 1646, 2037
BspCNI	CTCAG	5 cut(s)	670, 1613, 1650, 2077, 2119
BspEI	TCCGGA	2 cut(s)	1309, 1414
BspHI	TCATGA	2 cut(s)	912, 1030
BspLI	GGNNCC	3 cut(s)	298, 313, 1159
BspMI	ACCTGC	1 cut(s)	274
BspOI	GCTAGC	1 cut(s)	485
BspQI	GCTCTTC	1 cut(s)	1089
BspT104I	TTCGAA	2 cut(s)	226, 2003
BsrDI	GCAATG	1 cut(s)	1707
BsrFI	RCCGGY	1 cut(s)	590
BsrGI	TGTACA	1 cut(s)	369
BsrI	ACTGG	3 cut(s)	298, 1428, 2059
BssAI	RCCGGY	1 cut(s)	590
BssECI	CCNNGG	5 cut(s)	179, 180, 273, 561, 2038
BssMI	GATC	7 cut(s)	316, 706, 790, 915, 1369, 1517, 2208
BssSI	CACGAG	1 cut(s)	735
BssT1I	CCWWGG	1 cut(s)	561
Bst2BI	CACGAG	1 cut(s)	735
Bst2UI	CCWGG	4 cut(s)	181, 363, 1533, 2039
Bst4CI	ACNGT	4 cut(s)	571, 580, 697, 1147
Bst6I	CTCTTC	3 cut(s)	170, 1089, 1710
BstAUI	TGTACA	1 cut(s)	369
BstBI	TTCGAA	2 cut(s)	226, 2003
BstC8I	GCNNGC	5 cut(s)	147, 483, 1947, 2055, 2203
BstDEI	CTNAG	8 cut(s)	340, 549, 678, 923, 1600, 1658, 2085, 2127
BstF5I	GGATG	6 cut(s)	1411, 1423, 1696, 1836, 2125, 2147
BstH2I	RGCGCY	2 cut(s)	8, 1639
BstHHI	GCGC	2 cut(s)	7, 1638
BstKTI	GATC	7 cut(s)	319, 709, 793, 918, 1372, 1520, 2211
BstMAI	GTCTC	2 cut(s)	560, 1590
BstMBI	GATC	7 cut(s)	316, 706, 790, 915, 1369, 1517, 2208
BstMWI	GCNNNNNNNGC	9 cut(s)	210, 275, 294, 455, 1460, 1469, 1472, 1481, 2129
BstNI	CCWGG	4 cut(s)	181, 363, 1533, 2039
BstNSI	RCATGY	2 cut(s)	590, 1265
BstPAI	GACNNNNGTC	2 cut(s)	513, 1283
BstSCI	CCNGG	4 cut(s)	179, 361, 1531, 2037
BstV1I	GCAGC	5 cut(s)	200, 1459, 1475, 1709, 2110
BstV2I	GAAGAC	4 cut(s)	123, 129, 389, 826
BstXI	CCANNNNNNTGG	1 cut(s)	1169
BsuRI	GGCC	6 cut(s)	62, 361, 820, 1323, 1646, 2037
BtrI	CACGTC	1 cut(s)	1281
BtsCI	GGATG	6 cut(s)	1411, 1423, 1696, 1836, 2125, 2147
BtsIMutI	CAGTG	3 cut(s)	504, 1094, 1655
BveI	ACCTGC	1 cut(s)	274
Cac8I	GCNNGC	5 cut(s)	147, 483, 1947, 2055, 2203
CaiI	CAGNNNCTG	1 cut(s)	1817
CciI	TCATGA	2 cut(s)	912, 1030
CfoI	GCGC	2 cut(s)	7, 1638
Cfr10I	RCCGGY	1 cut(s)	590
Cfr13I	GGNCC	1 cut(s)	312
CseI	GACGC	2 cut(s)	1273, 1998
Csp6I	GTAC	3 cut(s)	370, 1421, 1842
CspCI	CAANNNNNGTGG	4 cut(s)	499, 534, 586, 621
CviAII	CATG	8 cut(s)	58, 587, 644, 767, 913, 1031, 1262, 2206
CviQI	GTAC	3 cut(s)	370, 1421, 1842
DdeI	CTNAG	8 cut(s)	340, 549, 678, 923, 1600, 1658, 2085, 2127
DpnI	GATC	7 cut(s)	318, 708, 792, 917, 1371, 1519, 2210
DpnII	GATC	7 cut(s)	316, 706, 790, 915, 1369, 1517, 2208
DrdI	GACNNNNNNGTC	2 cut(s)	393, 830
DseDI	GACNNNNNNGTC	2 cut(s)	393, 830
Eam1104I	CTCTTC	3 cut(s)	170, 1089, 1710
EarI	CTCTTC	3 cut(s)	170, 1089, 1710
EciI	GGCGGA	3 cut(s)	27, 80, 200
Ecl136II	GAGCTC	2 cut(s)	1992, 2185
Eco130I	CCWWGG	1 cut(s)	561
Eco147I	AGGCCT	1 cut(s)	2037
Eco24I	GRGCYC	2 cut(s)	1994, 2187
Eco32I	GATATC	2 cut(s)	937, 1871
Eco47I	GGWCC	1 cut(s)	312
Eco53kI	GAGCTC	2 cut(s)	1992, 2185
Eco57I	CTGAAG	4 cut(s)	1070, 1587, 1849, 1970
Eco88I	CYCGRG	1 cut(s)	2163
EcoICRI	GAGCTC	2 cut(s)	1992, 2185
EcoRI	GAATTC	2 cut(s)	782, 2194
EcoRII	CCWGG	4 cut(s)	179, 361, 1531, 2037
EcoRV	GATATC	2 cut(s)	937, 1871
EcoT14I	CCWWGG	1 cut(s)	561
EcoT22I	ATGCAT	1 cut(s)	1105
EcoT38I	GRGCYC	2 cut(s)	1994, 2187
ErhI	CCWWGG	1 cut(s)	561
FaeI	CATG	8 cut(s)	61, 590, 647, 770, 916, 1034, 1265, 2209
FalI	AAGNNNNNCTT	4 cut(s)	1374, 1406, 1959, 1991
FaqI	GGGAC	2 cut(s)	138, 399
FatI	CATG	8 cut(s)	57, 586, 643, 766, 912, 1030, 1261, 2205
FbaI	TGATCA	2 cut(s)	915, 1369
Fnu4HI	GCNGC	5 cut(s)	214, 1464, 1473, 1698, 2124
FokI	GGATG	6 cut(s)	1398, 1430, 1703, 1823, 2132, 2134
FriOI	GRGCYC	2 cut(s)	1994, 2187
Fsp4HI	GCNGC	5 cut(s)	214, 1464, 1473, 1698, 2124
FspBI	CTAG	4 cut(s)	482, 890, 1335, 1493
GlaI	GCGC	2 cut(s)	6, 1637
GluI	GCNGC	5 cut(s)	214, 1464, 1473, 1698, 2124
GsaI	CCCAGC	1 cut(s)	545
HaeII	RGCGCY	2 cut(s)	8, 1639
HaeIII	GGCC	6 cut(s)	62, 361, 820, 1323, 1646, 2037
HapII	CCGG	5 cut(s)	450, 591, 1310, 1415, 2176
HgaI	GACGC	2 cut(s)	1273, 1998
HhaI	GCGC	2 cut(s)	7, 1638
Hin1II	CATG	8 cut(s)	61, 590, 647, 770, 916, 1034, 1265, 2209
Hin6I	GCGC	2 cut(s)	5, 1636
HinP1I	GCGC	2 cut(s)	5, 1636
HincII	GTYRAC	3 cut(s)	436, 576, 1278
HindII	GTYRAC	3 cut(s)	436, 576, 1278
HinfI	GANTC	8 cut(s)	322, 523, 1065, 1427, 1571, 1598, 1720, 2080
HpaII	CCGG	5 cut(s)	450, 591, 1310, 1415, 2176
HphI	GGTGA	4 cut(s)	392, 1268, 1765, 1772
Hpy166II	GTNNAC	3 cut(s)	436, 576, 1278
Hpy8I	GTNNAC	3 cut(s)	436, 576, 1278
HpyCH4III	ACNGT	4 cut(s)	571, 580, 697, 1147
HpyCH4IV	ACGT	2 cut(s)	1280, 1786
HpyF10VI	GCNNNNNNNGC	9 cut(s)	210, 275, 294, 455, 1460, 1469, 1472, 1481, 2129
HpyF3I	CTNAG	8 cut(s)	340, 549, 678, 923, 1600, 1658, 2085, 2127
HpySE526I	ACGT	2 cut(s)	1280, 1786
Hsp92II	CATG	8 cut(s)	61, 590, 647, 770, 916, 1034, 1265, 2209
HspAI	GCGC	2 cut(s)	5, 1636
Kpn2I	TCCGGA	2 cut(s)	1309, 1414
Ksp22I	TGATCA	2 cut(s)	915, 1369
Kzo9I	GATC	7 cut(s)	316, 706, 790, 915, 1369, 1517, 2208
LguI	GCTCTTC	1 cut(s)	1089
LmnI	GCTCC	4 cut(s)	302, 378, 1997, 2182
Lsp1109I	GCAGC	5 cut(s)	200, 1459, 1475, 1709, 2110
LweI	GCATC	7 cut(s)	510, 816, 1090, 1420, 1804, 2040, 2110
MaeI	CTAG	4 cut(s)	482, 890, 1335, 1493
MaeII	ACGT	2 cut(s)	1280, 1786
MaeIII	GTNAC	3 cut(s)	691, 748, 1787
MalI	GATC	7 cut(s)	318, 708, 792, 917, 1371, 1519, 2210
MboI	GATC	7 cut(s)	316, 706, 790, 915, 1369, 1517, 2208
MhlI	GDGCHC	5 cut(s)	383, 1834, 1994, 2164, 2187
MluCI	AATT	7 cut(s)	188, 782, 879, 1313, 1668, 1731, 2194
MlyI	GAGTC	1 cut(s)	1592
MmeI	TCCRAC	2 cut(s)	551, 2205
Mph1103I	ATGCAT	1 cut(s)	1105
MroI	TCCGGA	2 cut(s)	1309, 1414
MroXI	GAANNNNTTC	1 cut(s)	1719
MseI	TTAA	6 cut(s)	1187, 1770, 1808, 1977, 2100, 2233
MslI	CAYNNNNRTG	4 cut(s)	591, 960, 1167, 1416
MspA1I	CMGCKG	1 cut(s)	1604
MspI	CCGG	5 cut(s)	450, 591, 1310, 1415, 2176
MspR9I	CCNGG	4 cut(s)	181, 363, 1533, 2039
Mva1269I	GAATGC	2 cut(s)	1456, 2011
MvaI	CCWGG	4 cut(s)	181, 363, 1533, 2039
MwoI	GCNNNNNNNGC	9 cut(s)	210, 275, 294, 455, 1460, 1469, 1472, 1481, 2129
NdeII	GATC	7 cut(s)	316, 706, 790, 915, 1369, 1517, 2208
NheI	GCTAGC	1 cut(s)	481
NlaIII	CATG	8 cut(s)	61, 590, 647, 770, 916, 1034, 1265, 2209
NlaIV	GGNNCC	3 cut(s)	298, 313, 1159
NmuCI	GTSAC	1 cut(s)	748
NsiI	ATGCAT	1 cut(s)	1105
NspI	RCATGY	2 cut(s)	590, 1265
NspV	TTCGAA	2 cut(s)	226, 2003
PagI	TCATGA	2 cut(s)	912, 1030
PaqCI	CACCTGC	1 cut(s)	274
PasI	CCCWGGG	1 cut(s)	180
PceI	AGGCCT	1 cut(s)	2037
PciSI	GCTCTTC	1 cut(s)	1089
PctI	GAATGC	2 cut(s)	1456, 2011
PdmI	GAANNNNTTC	1 cut(s)	1719
PfeI	GAWTC	7 cut(s)	322, 523, 1065, 1427, 1571, 1720, 2080
PkrI	GCNGC	5 cut(s)	215, 1465, 1474, 1699, 2125
PleI	GAGTC	1 cut(s)	1592
PpsI	GAGTC	1 cut(s)	1592
PshAI	GACNNNNGTC	2 cut(s)	513, 1283
Psp124BI	GAGCTC	2 cut(s)	1994, 2187
Psp6I	CCWGG	4 cut(s)	179, 361, 1531, 2037
PspFI	CCCAGC	1 cut(s)	541
PspGI	CCWGG	4 cut(s)	179, 361, 1531, 2037
PspN4I	GGNNCC	3 cut(s)	298, 313, 1159
PspPI	GGNCC	1 cut(s)	312
PstNI	CAGNNNCTG	1 cut(s)	1817
PvuII	CAGCTG	1 cut(s)	1604
RsaI	GTAC	3 cut(s)	371, 1422, 1843
RsaNI	GTAC	3 cut(s)	370, 1421, 1842
RseI	CAYNNNNRTG	4 cut(s)	591, 960, 1167, 1416
SacI	GAGCTC	2 cut(s)	1994, 2187
SapI	GCTCTTC	1 cut(s)	1089
SaqAI	TTAA	6 cut(s)	1187, 1770, 1808, 1977, 2100, 2233
SatI	GCNGC	5 cut(s)	214, 1464, 1473, 1698, 2124
Sau3AI	GATC	7 cut(s)	316, 706, 790, 915, 1369, 1517, 2208
Sau96I	GGNCC	1 cut(s)	312
SchI	GAGTC	1 cut(s)	1592
ScrFI	CCNGG	4 cut(s)	181, 363, 1533, 2039
SduI	GDGCHC	5 cut(s)	383, 1834, 1994, 2164, 2187
SfaNI	GCATC	7 cut(s)	510, 816, 1090, 1420, 1804, 2040, 2110
SfuI	TTCGAA	2 cut(s)	226, 2003
SinI	GGWCC	1 cut(s)	312
SmiMI	CAYNNNNRTG	4 cut(s)	591, 960, 1167, 1416
SmlI	CTYRAG	2 cut(s)	444, 1457
SmoI	CTYRAG	2 cut(s)	444, 1457
SpeI	ACTAGT	1 cut(s)	1492
Sse9I	AATT	7 cut(s)	188, 782, 879, 1313, 1668, 1731, 2194
SseBI	AGGCCT	1 cut(s)	2037
SsiI	CCGC	7 cut(s)	38, 91, 185, 221, 1025, 1357, 1919
SspI	AATATT	3 cut(s)	420, 847, 1955
SspMI	CTAG	4 cut(s)	482, 890, 1335, 1493
SstI	GAGCTC	2 cut(s)	1994, 2187
StuI	AGGCCT	1 cut(s)	2037
StyD4I	CCNGG	4 cut(s)	179, 361, 1531, 2037
StyI	CCWWGG	1 cut(s)	561
TaaI	ACNGT	4 cut(s)	571, 580, 697, 1147
TaiI	ACGT	2 cut(s)	1283, 1789
TaqI	TCGA	6 cut(s)	19, 226, 709, 2003, 2211, 2217
TaqII	GACCGA	1 cut(s)	1282
TasI	AATT	7 cut(s)	188, 782, 879, 1313, 1668, 1731, 2194
TatI	WGTACW	2 cut(s)	369, 1420
TfiI	GAWTC	7 cut(s)	322, 523, 1065, 1427, 1571, 1720, 2080
Tru1I	TTAA	6 cut(s)	1187, 1770, 1808, 1977, 2100, 2233
Tru9I	TTAA	6 cut(s)	1187, 1770, 1808, 1977, 2100, 2233
TscAI	CASTG	3 cut(s)	511, 1094, 1662
TseFI	GTSAC	1 cut(s)	748
TseI	GCWGC	5 cut(s)	213, 1463, 1472, 1697, 2123
Tsp45I	GTSAC	1 cut(s)	748
TspDTI	ATGAA	9 cut(s)	46, 429, 978, 1019, 1173, 1728, 1980, 2093, 2136
TspGWI	ACGGA	2 cut(s)	117, 1829
TspRI	CASTG	3 cut(s)	511, 1094, 1662
VpaK11BI	GGWCC	1 cut(s)	312
XapI	RAATTY	5 cut(s)	188, 782, 1313, 1668, 2194
XceI	RCATGY	2 cut(s)	590, 1265
XmnI	GAANNNNTTC	1 cut(s)	1719
XspI	CTAG	4 cut(s)	482, 890, 1335, 1493
Zsp2I	ATGCAT	1 cut(s)	1105

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.