Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0006623

Physical Location & Seq

Genomic Coordinates Forward (+)

9395585 .. 9405746

Gene Structure 10162 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g08270.16

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGGCGCTTCTTCTTCTTCGACCTCCTCCTCCTCCTCCGCCCTCTTATCTTCGCTTCATGGCCTTACGCTTCCTCTCCCACTCCTCTCTCCGCCCTCACTTCCCCTCTCCTTCTTTTTCTTCTTCTTCTTCGTCTTCGTCTTCCTCGCTTCCTCGTCCCTCTCTCCTCTTCAACCCCCCACCAAGGGCGGAATTTCCATTGAAGGGCGTTGGTAGCTGCTTTGCGGTTCGAAATGTGGTTTCTGCAAGAGCTTTTATGTCCTCTACACCTGCAACCGAGGCTTTTCAAGGCACTACTGGCTCCAGTGCTTATGGGTCCGATCAGATTCAGGTATTGGAAGGCTTAGACCCTGTTAGAAAGAGGCCAGGAATGTATATTGGGAGCACGGGACTTCGTGGTCTTCACCATTTGGTATATGAAATATTAGATAATGCAGTTGATGAGGCTCAAGCCGGATTTGCTTCAAAGGTTGAGGTCATTTTGCTAGCAGATGGTTCTGTGAGCATCACTGACAATGGTCGTGGGATTCCGACTGATATGCACCCAGCAACCAAGAAATCTTCCTTGGAGACTGTGTTGACGGTTTTACATGCTGGCGGCAAGTTTGGTGGTTCAAGTAGTGGCTATAGTGTGTCTGGTGGATTGCATGGTGTGGGGTTATCAGTCGTCAATGCCTTGTCTGAGGCATTAGAAGTTACTGTTTGGCGTGATAGAATGGAATATCAGCAGAAGTATTCTCGTGGAAAACCTGTGACAACTCTATCCTGTCATGTGCTTCCAGTTGAATTCCAAGATCGGCAAGGAACACGCATCAGATTTTGGCCTGACAAAGAAGTCTTCACAACTAATATTCAGTTTGACTACAACACGATAGCTGGACGAATTAGGGAACTAGCTTTTCTAAACCCAAACCTCATGATCACTCTTAGACAAGAGGATATCAATCCAGAGAAGAACCACCACAATGAATACTTTTATGCAGGGGGTTTGGTTGAATATGTAAAATGGCTAAATACTGATAAGAAACCGCTTCATGATGTGGTTGGCTTTAGAAAAGAAGTAGATGGAATCACTATTGATGTAGCTCTTCAGTGGTGTTCTGATGCATATTCAGATACAATGCTTGGGTATGCTAATAGCATACGAACTGTTGATGGAGGAACCCATATTGATGGAACGAAGGCTTCATTAACAAGAACTCTAAATAGTCTTGGAAAGAAGTCAAAACTTATCAAGGAAAAGGATATTACTTTGAGTGGTGAGCATGTGAGAGAAGGGTTGACGTGTGTCATCTCGGTCAAAGTTCCCAATCCGGAATTTGAAGGCCAAACAAAGACTAGGTTGGGAAATCCAGAGGTGCGGAGAGTGGTTGATCAATCACTTCAAGAGTATCTTACAGAATACTTGGAGTTGCATCCGGATGTACTGGATTCAATCCTTTCAAAGTCTTTGAATGCTCTCAAGGCAGCTTTGGCTGCAAAGAGGGCAAGAGAACTAGTCAGACAAAAAAGCGTGTTGAGATCATCTTCCCTGCCAGGAAAACTATCTGATTGCTCATCAACAAATCCTGAAGAATCAGAAATCTTTATAGTTGAAGGAGACTCAGCTGGTGGAAGTGCAAAACAAGGTCGTGATAGGCGCTTTCAGGCCATTCTGCCACTGAGGGGTAAAATTCTGAACATTGAAAGGAAGGATGAGGCAGCAATGTATAAAAATGAAGAGATTCAAAATCTAATTCTTGGTCTTGGACTTGGGGTGAAGGGTGAGGACTTCAAGAAGGAGGCTCTACGTTACCATAAGATTATCATTTTAACAGATGCTGATATAGATGGTGCTCACATCCGTACTCTGCTTCTGACATTTTTCTTCAGATATCAGAGAGCCTTATTTGATGAGGGCTACATATATGTTGGTGTTCCACCGCTTTACAAGGTTGAGAGGGGAAAGCAAGCCAAATATTGCTATGATGAAGCTGAACTTAAAATGCTTCAGAGCTCCTTCCCTTCAAATGCGTCTTACAACATTCAGAGGTTCAAAGGCCTGGGGGAAATGATGCCTGCCCAGTTATGGGAAACAACAATGAATCCTGAGCAAAGGCTTCTTAAACGATTAGGGGTCGAGGATGCTGCTGAGGCAAACATTGTGTTTTCATCCCTTATGGGTGCTCGGGTGGATTACCGGAAGGAGCTCATAAAGAATTCTGCGAGCATGATCGACCTCGATAAGTTGGATATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003916 GO:0003918 GO:0005575 GO:0006139 GO:0006259 GO:0006265 GO:0006725 GO:0006807 GO:0006996 GO:0007059 GO:0008094 GO:0008150 GO:0008152 GO:0009295 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0017111 GO:0034641 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0051276 GO:0061505 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_gyraseB DNA_gyraseB_C HATPase_c Toprim

Protein Analysis

746

Amino Acids

82.62

Weight (kDa)

8.43

Isoelectric Point (pI)

43.72

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10270 AT3G10270 AT5G04130
fragaria_vesca	FvH4_6g25390 FvH4_6g25390 FvH4_6g25390
malus_domestica	MD12G1095800.v1.1 MD14G1090600.v1.1
prunus_persica	Prupe.7G033800_v2.0.a1
pyrus_communis	pycom12g08270
rosa_chinensis	RchiOBHm_Chr2g0125831
rosa_laevigata	RLG00000018881
rosa_multiflora	Rmu_co8103446.1_g000001 Rmu_sc0009079.1_g000002
rosa_roxburghii	Rroxscaffold_2G00117620 Rroxscaffold_2G00117680 Rroxscaffold_2G00125340 Rroxscaffold_2G00125370 Rroxscaffold_3G00238600 Rroxscaffold_4G00318520 Rroxscaffold_4G00324150 Rroxscaffold_5G00337460
rosa_rugosa	Rorug02G0259400
rosa_samantha	Rh2AG317700 Rh2BG326100
rosa_wichuraiana	Rw2G025700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	277
AasI	GACNNNNNNGTC	2 cut(s)	396, 833
Acc36I	ACCTGC	1 cut(s)	277
AccIII	TCCGGA	2 cut(s)	1312, 1417
AciI	CCGC	8 cut(s)	38, 91, 188, 224, 597, 1028, 1360, 1922
AcsI	RAATTY	5 cut(s)	191, 785, 1316, 1671, 2197
AcuI	CTGAAG	4 cut(s)	1073, 1590, 1852, 1973
AfaI	GTAC	2 cut(s)	1425, 1846
AfiI	CCNNNNNNNGG	6 cut(s)	183, 184, 203, 1664, 2067, 2068
AflIII	ACRYGT	1 cut(s)	1283
AhlI	ACTAGT	1 cut(s)	1495
AjiI	CACGTC	1 cut(s)	1284
AjnI	CCWGG	3 cut(s)	364, 1534, 2040
AjuI	GAANNNNNNNTTGG	2 cut(s)	548, 580
Alw21I	GWGCWC	5 cut(s)	386, 1837, 1997, 2167, 2190
Alw26I	GTCTC	2 cut(s)	563, 1593
AlwNI	CAGNNNCTG	1 cut(s)	1820
Ama87I	CYCGRG	1 cut(s)	2166
Aor13HI	TCCGGA	2 cut(s)	1312, 1417
AoxI	GGCC	6 cut(s)	60, 362, 821, 1324, 1647, 2038
ApeKI	GCWGC	5 cut(s)	216, 1466, 1475, 1700, 2126
ApoI	RAATTY	5 cut(s)	191, 785, 1316, 1671, 2197
Asp700I	GAANNNNTTC	1 cut(s)	1722
AspLEI	GCGC	2 cut(s)	7, 1641
AspS9I	GGNCC	1 cut(s)	315
AsuHPI	GGTGA	4 cut(s)	395, 1271, 1768, 1775
AsuII	TTCGAA	1 cut(s)	229
AsuNHI	GCTAGC	1 cut(s)	484
AvaI	CYCGRG	1 cut(s)	2166
AvaII	GGWCC	1 cut(s)	315
BanII	GRGCYC	2 cut(s)	1997, 2190
BauI	CACGAG	1 cut(s)	738
BbsI	GAAGAC	4 cut(s)	126, 132, 392, 829
Bbv12I	GWGCWC	5 cut(s)	386, 1837, 1997, 2167, 2190
BbvCI	CCTCAGC	1 cut(s)	2130
BbvI	GCAGC	5 cut(s)	203, 1462, 1478, 1712, 2113
BccI	CCATC	5 cut(s)	485, 1058, 1148, 1166, 1823
BcgI	CGANNNNNNTGC	4 cut(s)	520, 554, 2108, 2142
BciT130I	CCWGG	3 cut(s)	366, 1536, 2042
BclI	TGATCA	2 cut(s)	918, 1372
BcoDI	GTCTC	2 cut(s)	563, 1593
BcuI	ACTAGT	1 cut(s)	1495
BfaI	CTAG	4 cut(s)	485, 893, 1338, 1496
BfmI	CTRYAG	1 cut(s)	625
BfoI	RGCGCY	2 cut(s)	8, 1642
BfuAI	ACCTGC	1 cut(s)	277
BisI	GCNGC	6 cut(s)	217, 598, 1467, 1476, 1701, 2127
BlsI	GCNGC	6 cut(s)	218, 599, 1468, 1477, 1702, 2128
Bme1390I	CCNGG	3 cut(s)	366, 1536, 2042
Bme18I	GGWCC	1 cut(s)	315
BmeT110I	CYCGRG	1 cut(s)	2166
BmgBI	CACGTC	1 cut(s)	1284
BmgT120I	GGNCC	1 cut(s)	315
BmiI	GGNNCC	3 cut(s)	301, 316, 1162
BmrFI	CCNGG	3 cut(s)	366, 1536, 2042
BmrI	ACTGGG	1 cut(s)	2056
BmsI	GCATC	7 cut(s)	513, 819, 1093, 1423, 1807, 2043, 2113
BmtI	GCTAGC	1 cut(s)	488
BmuI	ACTGGG	1 cut(s)	2056
BoxI	GACNNNNGTC	2 cut(s)	516, 1286
BpiI	GAAGAC	4 cut(s)	126, 132, 392, 829
BpmI	CTGGAG	1 cut(s)	286
Bpu10I	CCTNAGC	2 cut(s)	2088, 2130
Bpu14I	TTCGAA	1 cut(s)	229
BpuEI	CTTGAG	2 cut(s)	432, 1445
BsaBI	GATNNNNATC	1 cut(s)	942
BsaJI	CCNNGG	4 cut(s)	182, 276, 564, 2041
BsaWI	WCCGGW	3 cut(s)	1312, 1417, 2178
BsaXI	ACNNNNNCTCC	2 cut(s)	560, 590
Bsc4I	CCNNNNNNNGG	6 cut(s)	183, 184, 203, 1664, 2067, 2068
Bse1I	ACTGG	5 cut(s)	301, 303, 779, 1431, 2062
Bse3DI	GCAATG	1 cut(s)	1710
Bse8I	GATNNNNATC	1 cut(s)	942
BseAI	TCCGGA	2 cut(s)	1312, 1417
BseBI	CCWGG	3 cut(s)	366, 1536, 2042
BseDI	CCNNGG	4 cut(s)	182, 276, 564, 2041
BseGI	GGATG	6 cut(s)	1414, 1426, 1699, 1839, 2128, 2150
BseJI	GATNNNNATC	1 cut(s)	942
BseLI	CCNNNNNNNGG	6 cut(s)	183, 184, 203, 1664, 2067, 2068
BseMI	GCAATG	1 cut(s)	1710
BseMII	CTCAG	5 cut(s)	672, 1617, 1652, 2079, 2121
BseNI	ACTGG	5 cut(s)	301, 303, 779, 1431, 2062
BseRI	GAGGAG	6 cut(s)	15, 18, 21, 24, 73, 155
BseXI	GCAGC	5 cut(s)	203, 1462, 1478, 1712, 2113
BseYI	CCCAGC	1 cut(s)	544
BshFI	GGCC	6 cut(s)	62, 364, 823, 1326, 1649, 2040
BsiHKAI	GWGCWC	5 cut(s)	386, 1837, 1997, 2167, 2190
BsiHKCI	CYCGRG	1 cut(s)	2166
BsiSI	CCGG	4 cut(s)	453, 1313, 1418, 2179
BslFI	GGGAC	2 cut(s)	141, 402
BslI	CCNNNNNNNGG	6 cut(s)	183, 184, 203, 1664, 2067, 2068
BsmAI	GTCTC	2 cut(s)	563, 1593
BsmFI	GGGAC	2 cut(s)	141, 402
BsmI	GAATGC	1 cut(s)	1459
BsnI	GGCC	6 cut(s)	62, 364, 823, 1326, 1649, 2040
BsoBI	CYCGRG	1 cut(s)	2166
Bsp119I	TTCGAA	1 cut(s)	229
Bsp1286I	GDGCHC	5 cut(s)	386, 1837, 1997, 2167, 2190
Bsp13I	TCCGGA	2 cut(s)	1312, 1417
Bsp143I	GATC	6 cut(s)	319, 793, 918, 1372, 1520, 2211
BspACI	CCGC	8 cut(s)	38, 91, 188, 224, 597, 1028, 1360, 1922
BspANI	GGCC	6 cut(s)	62, 364, 823, 1326, 1649, 2040
BspCNI	CTCAG	5 cut(s)	673, 1616, 1653, 2080, 2122
BspEI	TCCGGA	2 cut(s)	1312, 1417
BspHI	TCATGA	2 cut(s)	915, 1033
BspLI	GGNNCC	3 cut(s)	301, 316, 1162
BspMI	ACCTGC	1 cut(s)	277
BspOI	GCTAGC	1 cut(s)	488
BspQI	GCTCTTC	1 cut(s)	1092
BspT104I	TTCGAA	1 cut(s)	229
BsrDI	GCAATG	1 cut(s)	1710
BsrI	ACTGG	5 cut(s)	301, 303, 779, 1431, 2062
BssECI	CCNNGG	4 cut(s)	182, 276, 564, 2041
BssMI	GATC	6 cut(s)	319, 793, 918, 1372, 1520, 2211
BssSI	CACGAG	1 cut(s)	738
BssT1I	CCWWGG	2 cut(s)	182, 564
Bst2BI	CACGAG	1 cut(s)	738
Bst2UI	CCWGG	3 cut(s)	366, 1536, 2042
Bst4CI	ACNGT	4 cut(s)	574, 583, 700, 1150
Bst6I	CTCTTC	3 cut(s)	173, 1092, 1713
BstBI	TTCGAA	1 cut(s)	229
BstC8I	GCNNGC	5 cut(s)	486, 595, 1950, 2058, 2206
BstDEI	CTNAG	7 cut(s)	343, 681, 926, 1603, 1661, 2088, 2130
BstF5I	GGATG	6 cut(s)	1414, 1426, 1699, 1839, 2128, 2150
BstH2I	RGCGCY	2 cut(s)	8, 1642
BstHHI	GCGC	2 cut(s)	7, 1641
BstKTI	GATC	6 cut(s)	322, 796, 921, 1375, 1523, 2214
BstMAI	GTCTC	2 cut(s)	563, 1593
BstMBI	GATC	6 cut(s)	319, 793, 918, 1372, 1520, 2211
BstMWI	GCNNNNNNNGC	9 cut(s)	213, 278, 297, 458, 1463, 1472, 1475, 1484, 2132
BstNI	CCWGG	3 cut(s)	366, 1536, 2042
BstNSI	RCATGY	2 cut(s)	593, 1268
BstPAI	GACNNNNGTC	2 cut(s)	516, 1286
BstSCI	CCNGG	3 cut(s)	364, 1534, 2040
BstSFI	CTRYAG	1 cut(s)	625
BstV1I	GCAGC	5 cut(s)	203, 1462, 1478, 1712, 2113
BstV2I	GAAGAC	4 cut(s)	126, 132, 392, 829
BstXI	CCANNNNNNTGG	1 cut(s)	1172
BsuRI	GGCC	6 cut(s)	62, 364, 823, 1326, 1649, 2040
BtrI	CACGTC	1 cut(s)	1284
BtsCI	GGATG	6 cut(s)	1414, 1426, 1699, 1839, 2128, 2150
BtsIMutI	CAGTG	4 cut(s)	310, 507, 1097, 1658
BveI	ACCTGC	1 cut(s)	277
Cac8I	GCNNGC	5 cut(s)	486, 595, 1950, 2058, 2206
CaiI	CAGNNNCTG	1 cut(s)	1820
CciI	TCATGA	2 cut(s)	915, 1033
CfoI	GCGC	2 cut(s)	7, 1641
Cfr13I	GGNCC	1 cut(s)	315
CseI	GACGC	1 cut(s)	2001
Csp6I	GTAC	2 cut(s)	1424, 1845
CspCI	CAANNNNNGTGG	4 cut(s)	502, 537, 589, 624
CviAII	CATG	8 cut(s)	58, 590, 647, 770, 916, 1034, 1265, 2209
CviQI	GTAC	2 cut(s)	1424, 1845
DdeI	CTNAG	7 cut(s)	343, 681, 926, 1603, 1661, 2088, 2130
DpnI	GATC	6 cut(s)	321, 795, 920, 1374, 1522, 2213
DpnII	GATC	6 cut(s)	319, 793, 918, 1372, 1520, 2211
DrdI	GACNNNNNNGTC	2 cut(s)	396, 833
DseDI	GACNNNNNNGTC	2 cut(s)	396, 833
Eam1104I	CTCTTC	3 cut(s)	173, 1092, 1713
EarI	CTCTTC	3 cut(s)	173, 1092, 1713
EciI	GGCGGA	3 cut(s)	27, 80, 203
Ecl136II	GAGCTC	2 cut(s)	1995, 2188
Eco130I	CCWWGG	2 cut(s)	182, 564
Eco147I	AGGCCT	1 cut(s)	2040
Eco24I	GRGCYC	2 cut(s)	1997, 2190
Eco32I	GATATC	2 cut(s)	940, 1874
Eco47I	GGWCC	1 cut(s)	315
Eco53kI	GAGCTC	2 cut(s)	1995, 2188
Eco57I	CTGAAG	4 cut(s)	1073, 1590, 1852, 1973
Eco88I	CYCGRG	1 cut(s)	2166
EcoICRI	GAGCTC	2 cut(s)	1995, 2188
EcoRI	GAATTC	2 cut(s)	785, 2197
EcoRII	CCWGG	3 cut(s)	364, 1534, 2040
EcoRV	GATATC	2 cut(s)	940, 1874
EcoT14I	CCWWGG	2 cut(s)	182, 564
EcoT22I	ATGCAT	1 cut(s)	1108
EcoT38I	GRGCYC	2 cut(s)	1997, 2190
ErhI	CCWWGG	2 cut(s)	182, 564
FaeI	CATG	8 cut(s)	61, 593, 650, 773, 919, 1037, 1268, 2212
FalI	AAGNNNNNCTT	4 cut(s)	1377, 1409, 1962, 1994
FaqI	GGGAC	2 cut(s)	141, 402
FatI	CATG	8 cut(s)	57, 589, 646, 769, 915, 1033, 1264, 2208
FbaI	TGATCA	2 cut(s)	918, 1372
Fnu4HI	GCNGC	6 cut(s)	217, 598, 1467, 1476, 1701, 2127
FokI	GGATG	6 cut(s)	1401, 1433, 1706, 1826, 2135, 2137
FriOI	GRGCYC	2 cut(s)	1997, 2190
Fsp4HI	GCNGC	6 cut(s)	217, 598, 1467, 1476, 1701, 2127
FspBI	CTAG	4 cut(s)	485, 893, 1338, 1496
GlaI	GCGC	2 cut(s)	6, 1640
GluI	GCNGC	6 cut(s)	217, 598, 1467, 1476, 1701, 2127
GsaI	CCCAGC	1 cut(s)	548
GsuI	CTGGAG	1 cut(s)	286
HaeII	RGCGCY	2 cut(s)	8, 1642
HaeIII	GGCC	6 cut(s)	62, 364, 823, 1326, 1649, 2040
HapII	CCGG	4 cut(s)	453, 1313, 1418, 2179
HgaI	GACGC	1 cut(s)	2001
HhaI	GCGC	2 cut(s)	7, 1641
Hin1II	CATG	8 cut(s)	61, 593, 650, 773, 919, 1037, 1268, 2212
Hin6I	GCGC	2 cut(s)	5, 1639
HinP1I	GCGC	2 cut(s)	5, 1639
HincII	GTYRAC	2 cut(s)	579, 1281
HindII	GTYRAC	2 cut(s)	579, 1281
HinfI	GANTC	8 cut(s)	325, 526, 1068, 1430, 1574, 1601, 1723, 2083
HpaII	CCGG	4 cut(s)	453, 1313, 1418, 2179
HphI	GGTGA	4 cut(s)	395, 1271, 1768, 1775
Hpy166II	GTNNAC	2 cut(s)	579, 1281
Hpy8I	GTNNAC	2 cut(s)	579, 1281
HpyCH4III	ACNGT	4 cut(s)	574, 583, 700, 1150
HpyCH4IV	ACGT	2 cut(s)	1283, 1789
HpyF10VI	GCNNNNNNNGC	9 cut(s)	213, 278, 297, 458, 1463, 1472, 1475, 1484, 2132
HpyF3I	CTNAG	7 cut(s)	343, 681, 926, 1603, 1661, 2088, 2130
HpySE526I	ACGT	2 cut(s)	1283, 1789
Hsp92II	CATG	8 cut(s)	61, 593, 650, 773, 919, 1037, 1268, 2212
HspAI	GCGC	2 cut(s)	5, 1639
Kpn2I	TCCGGA	2 cut(s)	1312, 1417
Ksp22I	TGATCA	2 cut(s)	918, 1372
Kzo9I	GATC	6 cut(s)	319, 793, 918, 1372, 1520, 2211
LguI	GCTCTTC	1 cut(s)	1092
LmnI	GCTCC	4 cut(s)	305, 381, 2000, 2185
Lsp1109I	GCAGC	5 cut(s)	203, 1462, 1478, 1712, 2113
LweI	GCATC	7 cut(s)	513, 819, 1093, 1423, 1807, 2043, 2113
MaeI	CTAG	4 cut(s)	485, 893, 1338, 1496
MaeII	ACGT	2 cut(s)	1283, 1789
MaeIII	GTNAC	3 cut(s)	694, 751, 1790
MalI	GATC	6 cut(s)	321, 795, 920, 1374, 1522, 2213
MboI	GATC	6 cut(s)	319, 793, 918, 1372, 1520, 2211
MhlI	GDGCHC	5 cut(s)	386, 1837, 1997, 2167, 2190
MluCI	AATT	7 cut(s)	191, 785, 882, 1316, 1671, 1734, 2197
MlyI	GAGTC	1 cut(s)	1595
MmeI	TCCRAC	2 cut(s)	554, 2208
Mph1103I	ATGCAT	1 cut(s)	1108
MroI	TCCGGA	2 cut(s)	1312, 1417
MroXI	GAANNNNTTC	1 cut(s)	1722
MseI	TTAA	5 cut(s)	1190, 1811, 1980, 2103, 2236
MslI	CAYNNNNRTG	3 cut(s)	963, 1170, 1419
MspA1I	CMGCKG	1 cut(s)	1607
MspI	CCGG	4 cut(s)	453, 1313, 1418, 2179
MspR9I	CCNGG	3 cut(s)	366, 1536, 2042
Mva1269I	GAATGC	1 cut(s)	1459
MvaI	CCWGG	3 cut(s)	366, 1536, 2042
MwoI	GCNNNNNNNGC	9 cut(s)	213, 278, 297, 458, 1463, 1472, 1475, 1484, 2132
NdeII	GATC	6 cut(s)	319, 793, 918, 1372, 1520, 2211
NheI	GCTAGC	1 cut(s)	484
NlaIII	CATG	8 cut(s)	61, 593, 650, 773, 919, 1037, 1268, 2212
NlaIV	GGNNCC	3 cut(s)	301, 316, 1162
NmuCI	GTSAC	1 cut(s)	751
NsiI	ATGCAT	1 cut(s)	1108
NspI	RCATGY	2 cut(s)	593, 1268
NspV	TTCGAA	1 cut(s)	229
PagI	TCATGA	2 cut(s)	915, 1033
PaqCI	CACCTGC	1 cut(s)	277
PceI	AGGCCT	1 cut(s)	2040
PciSI	GCTCTTC	1 cut(s)	1092
PctI	GAATGC	1 cut(s)	1459
PdmI	GAANNNNTTC	1 cut(s)	1722
PfeI	GAWTC	7 cut(s)	325, 526, 1068, 1430, 1574, 1723, 2083
PkrI	GCNGC	6 cut(s)	218, 599, 1468, 1477, 1702, 2128
PleI	GAGTC	1 cut(s)	1595
PpsI	GAGTC	1 cut(s)	1595
PshAI	GACNNNNGTC	2 cut(s)	516, 1286
Psp124BI	GAGCTC	2 cut(s)	1997, 2190
Psp6I	CCWGG	3 cut(s)	364, 1534, 2040
PspFI	CCCAGC	1 cut(s)	544
PspGI	CCWGG	3 cut(s)	364, 1534, 2040
PspN4I	GGNNCC	3 cut(s)	301, 316, 1162
PspPI	GGNCC	1 cut(s)	315
PstNI	CAGNNNCTG	1 cut(s)	1820
PvuII	CAGCTG	1 cut(s)	1607
RsaI	GTAC	2 cut(s)	1425, 1846
RsaNI	GTAC	2 cut(s)	1424, 1845
RseI	CAYNNNNRTG	3 cut(s)	963, 1170, 1419
SacI	GAGCTC	2 cut(s)	1997, 2190
SapI	GCTCTTC	1 cut(s)	1092
SaqAI	TTAA	5 cut(s)	1190, 1811, 1980, 2103, 2236
SatI	GCNGC	6 cut(s)	217, 598, 1467, 1476, 1701, 2127
Sau3AI	GATC	6 cut(s)	319, 793, 918, 1372, 1520, 2211
Sau96I	GGNCC	1 cut(s)	315
SchI	GAGTC	1 cut(s)	1595
ScrFI	CCNGG	3 cut(s)	366, 1536, 2042
SduI	GDGCHC	5 cut(s)	386, 1837, 1997, 2167, 2190
SfaNI	GCATC	7 cut(s)	513, 819, 1093, 1423, 1807, 2043, 2113
SfcI	CTRYAG	1 cut(s)	625
SfuI	TTCGAA	1 cut(s)	229
SinI	GGWCC	1 cut(s)	315
SmiMI	CAYNNNNRTG	3 cut(s)	963, 1170, 1419
SmlI	CTYRAG	2 cut(s)	447, 1460
SmoI	CTYRAG	2 cut(s)	447, 1460
SpeI	ACTAGT	1 cut(s)	1495
Sse9I	AATT	7 cut(s)	191, 785, 882, 1316, 1671, 1734, 2197
SseBI	AGGCCT	1 cut(s)	2040
SsiI	CCGC	8 cut(s)	38, 91, 188, 224, 597, 1028, 1360, 1922
SspI	AATATT	3 cut(s)	423, 850, 1958
SspMI	CTAG	4 cut(s)	485, 893, 1338, 1496
SstI	GAGCTC	2 cut(s)	1997, 2190
StuI	AGGCCT	1 cut(s)	2040
StyD4I	CCNGG	3 cut(s)	364, 1534, 2040
StyI	CCWWGG	2 cut(s)	182, 564
TaaI	ACNGT	4 cut(s)	574, 583, 700, 1150
TaiI	ACGT	2 cut(s)	1286, 1792
TaqI	TCGA	5 cut(s)	19, 229, 2118, 2214, 2220
TaqII	GACCGA	1 cut(s)	1285
TasI	AATT	7 cut(s)	191, 785, 882, 1316, 1671, 1734, 2197
TatI	WGTACW	1 cut(s)	1423
TauI	GCSGC	1 cut(s)	600
TfiI	GAWTC	7 cut(s)	325, 526, 1068, 1430, 1574, 1723, 2083
Tru1I	TTAA	5 cut(s)	1190, 1811, 1980, 2103, 2236
Tru9I	TTAA	5 cut(s)	1190, 1811, 1980, 2103, 2236
TscAI	CASTG	4 cut(s)	310, 514, 1097, 1665
TseFI	GTSAC	1 cut(s)	751
TseI	GCWGC	5 cut(s)	216, 1466, 1475, 1700, 2126
Tsp45I	GTSAC	1 cut(s)	751
TspDTI	ATGAA	9 cut(s)	46, 432, 981, 1022, 1176, 1731, 1983, 2096, 2139
TspGWI	ACGGA	1 cut(s)	1832
TspRI	CASTG	4 cut(s)	310, 514, 1097, 1665
VpaK11BI	GGWCC	1 cut(s)	315
XapI	RAATTY	5 cut(s)	191, 785, 1316, 1671, 2197
XceI	RCATGY	2 cut(s)	593, 1268
XmnI	GAANNNNTTC	1 cut(s)	1722
XspI	CTAG	4 cut(s)	485, 893, 1338, 1496
Zsp2I	ATGCAT	1 cut(s)	1108

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.