Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0006623

Physical Location & Seq

Genomic Coordinates Forward (+)

38773017 .. 38786964

Gene Structure 13948 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G025700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2190 bp

ATGGCGCTTGTTCTTCTGAGACTGAGAGCTCATTCTCCTAGTCCTCCTTATCTTCTTCGCTTCATGGCCTTACGCTTCATGTCACACTCATGTCTCCACCCTCACTCTTCCTCCCTCTTCTTCAGCCCACCAGCACCACCCAGGATTGGGCTTCGATTGAAGGACGTTCCTAGCTGCTGTGCTGCGGTTCGAAATGTGAGAGCTTTCATGTCGTCCAGCTCGGTAACCGAGGCTTTTCAAGGAAGCGCTGCCTCCACGGCTTATGGCGCTGATCAGATTCAGGTTTTAGAAGGCTTAGAGGCTGTTAGAAAGAGGCCCGGAATGTATATTGGGGACACCGGATTTCGTGGTCTTCATCATCTGGTTTATGAAATACTAGACAATGCTGTTGATGAGGCTCAAGGTGGATATGCTTCAAAGGTTGAGGTCATTTTACTGGCCGACAATTCTGTCAGCATCTCAGACAACGGCCGTGGGATACCTACTGATATGCATCCTGTAACCAAGAAATCTACATTGGAGACTGTGTTGACGGTTTTGCATGCTGGTGGCAAGTTTGGTGGTTCAAGTAGTGGCTATAGTGTGTCTGGTGGATTACATGGTGTGGGTTTATCAGTCGTTAATGCCTTGTCTGAGGCATTAGAAGTTACTATTTGGCGTGATGGAATGGAATACCAGCAAAAGTATTCTCGTGGAAAGCCTGTGACAACTCTCTCTTGTCATGTGCTTCCAATTGAATTTAAAGATAGACAAGGAACACGAATCAGATTCTTGCCTGACAAACAAGTATTTAAAACTCTTAATCAGTTTGACTACCACACAATTGCTGGAAGAATTAGGGAGCTAGCTTTTTTAAACCCTAATCTCATGATCACTCTTAGACAAGAGGATACGGATCCAGAGAAAATCCAGCACAATGAATACTTCTATGCAGGGGGTTTGGTTGAATATGTTAGATGGCTAAATACTGATAAGAAACCTCTTCATGAAGTGGTGGGTTTCAGAAAAGAAGTAGATGGAATCACTATTAATGTAGCTCTCCAGTGGTGTTCTGATGCATATTCAGATACAGTGCTAGGGTATGCTAATAGCATACGTACTGCTGACGGAGGAACCCATATTGATGGACTGAAAGCTTCGTTAACAAGAACGCTGAATAACCTTGGGAAGAAGTCAAAGCTTATCAAGGATAAAGATATTACCTTAAGTGGTGAGCATGTAAGAGAAGGGCTGACATGTGTAGTTTCAGTCAAAGTTCCCAATCCAGAGTTTGAAGGCCAGACAAAGACTAGGTTGGGAAATCCAGAGGTGCGAAGAGTGGTTGATAATTCTCTTCAAGAGTATCTTACAGAATACTTGGAGTTACATCCTGATGTACTGGATTCAATCCTTTCCAAGTCCTTAAATGCTCTCAAGGCAGCTCTGGCTGCAAAGAGGGCAAGGGAACTAGTCAGACAAAAGAGTGTTTTGCGTTCGTCTTCTCTCCCAGGAAAGCTAGCTGATTGCTCATCAACCAATCCTGAAGAATCTGAAATCTTTATAGTTGAAGGAGATTCAGCTGGAGGAAGTGCAAAGCAAGGTCGTGATAGACGCTTTCAGGCAATTCTTCCACTAAGGGGTAAAATTCTGAACATTGAAAGGAGGGACGAGGCAGCGATGTATAAAAATGAAGAAATTCAAAATCTGATTCTTGGACTTGGACTTGGGGTGAAGGGAGAGGACTTTAAAAAGGAAGCTCTGCGTTACCATAAGATTATCATCTTAACTGATGCTGATGTAGATGGTGCTCACATCCGAACTCTGCTACTGACTTTTTTCTTCAGATATCAGAGAGCCTTATTTGATGAGGGTTGCATATATGTTGGTGTTCCACCCTTTTACAAGGTTGAAAGGGGAAAGCAAGCATATTATTGCTATGATGACGCTGAACTTAAAAAGCTTCAGAGTTCCTTCCCTTCAAATGCATCATACAGCATTCAAAGGTTCAAAGGCCTGGGAGAAATGATGCCCACACAATTATGGGAAACAACAATGGATCCTCAAAAAAGGCTACTGAAACAATTAAGTGTGGATGATGCTGCTGAGGCTAATTTTGTCTTTTCCTCCCTTATGGGTTCTCGGGTGGATTTCCGGAAGGAACTCATTCAAAATGCTGCACGCATGATCAGCTTGGATCAGTTAGATATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003916 GO:0003918 GO:0005575 GO:0006139 GO:0006259 GO:0006265 GO:0006725 GO:0006807 GO:0006996 GO:0007059 GO:0008094 GO:0008150 GO:0008152 GO:0009295 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0017111 GO:0034641 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0051276 GO:0061505 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_gyraseB DNA_gyraseB_C HATPase_c Toprim

Protein Analysis

729

Amino Acids

81.14

Weight (kDa)

8.48

Isoelectric Point (pI)

41.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HATPase_c	PF02518	117 - 259	4.9e-17	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
DNA_gyraseB	PF00204	313 - 482	3.6e-55	DNA gyrase B
Toprim	PF01751	511 - 611	5.4e-18	Toprim domain
DNA_gyraseB_C	PF00986	655 - 716	5.8e-25	DNA gyrase B subunit, carboxyl terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10270 AT3G10270 AT5G04130
fragaria_vesca	FvH4_6g25390 FvH4_6g25390 FvH4_6g25390
malus_domestica	MD12G1095800.v1.1 MD14G1090600.v1.1
prunus_persica	Prupe.7G033800_v2.0.a1
pyrus_communis	pycom12g08270
rosa_chinensis	RchiOBHm_Chr2g0125831
rosa_laevigata	RLG00000018881
rosa_multiflora	Rmu_co8103446.1_g000001 Rmu_sc0009079.1_g000002
rosa_roxburghii	Rroxscaffold_2G00117620 Rroxscaffold_2G00117680 Rroxscaffold_2G00125340 Rroxscaffold_2G00125370 Rroxscaffold_3G00238600 Rroxscaffold_4G00318520 Rroxscaffold_4G00324150 Rroxscaffold_5G00337460
rosa_rugosa	Rorug02G0259400
rosa_samantha	Rh2AG317700 Rh2BG326100
rosa_wichuraiana	Rw2G025700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	449
AccIII	TCCGGA	1 cut(s)	2130
AciI	CCGC	1 cut(s)	185
AclWI	GGATC	5 cut(s)	890, 903, 2030, 2043, 2181
AcoI	YGGCCR	2 cut(s)	438, 469
AcsI	RAATTY	3 cut(s)	737, 1623, 1674
AcuI	CTGAAG	4 cut(s)	106, 1542, 1804, 1925
AfaI	GTAC	2 cut(s)	1099, 1377
AfeI	AGCGCT	1 cut(s)	247
AfiI	CCNNNNNNNGG	3 cut(s)	146, 147, 1616
AflII	CTTAAG	1 cut(s)	1204
AflIII	ACRYGT	1 cut(s)	1235
AhlI	ACTAGT	1 cut(s)	1447
AjnI	CCWGG	3 cut(s)	140, 1486, 1992
AjuI	GAANNNNNNNTTGG	2 cut(s)	500, 532
Alw21I	GWGCWC	2 cut(s)	31, 1789
Alw26I	GTCTC	3 cut(s)	13, 98, 515
AlwI	GGATC	5 cut(s)	890, 903, 2030, 2043, 2181
Ama87I	CYCGRG	1 cut(s)	2118
Aor13HI	TCCGGA	1 cut(s)	2130
Aor51HI	AGCGCT	1 cut(s)	247
AoxI	GGCC	6 cut(s)	66, 314, 438, 469, 1276, 1990
ApeKI	GCWGC	8 cut(s)	174, 182, 248, 1418, 1427, 1652, 2078, 2153
ApoI	RAATTY	3 cut(s)	737, 1623, 1674
AseI	ATTAAT	1 cut(s)	1029
Asp700I	GAANNNNTTC	2 cut(s)	1674, 2142
AspLEI	GCGC	3 cut(s)	7, 248, 269
AspS9I	GGNCC	1 cut(s)	315
AsuC2I	CCSGG	1 cut(s)	318
AsuHPI	GGTGA	2 cut(s)	1223, 1720
AsuII	TTCGAA	1 cut(s)	190
AsuNHI	GCTAGC	2 cut(s)	844, 1495
AvaI	CYCGRG	1 cut(s)	2118
BamHI	GGATCC	2 cut(s)	895, 2035
BanII	GRGCYC	1 cut(s)	31
BauI	CACGAG	1 cut(s)	690
BbsI	GAAGAC	2 cut(s)	344, 1470
Bbv12I	GWGCWC	2 cut(s)	31, 1789
BbvCI	CCTCAGC	1 cut(s)	2082
BbvI	GCAGC	8 cut(s)	161, 169, 235, 1414, 1430, 1664, 2065, 2140
BccI	CCATC	5 cut(s)	656, 951, 1010, 1118, 1775
BceAI	ACGGC	3 cut(s)	273, 456, 484
BciT130I	CCWGG	3 cut(s)	142, 1488, 1994
BciVI	GTATCC	2 cut(s)	471, 883
BclI	TGATCA	3 cut(s)	271, 870, 2163
BcnI	CCSGG	1 cut(s)	318
BcoDI	GTCTC	3 cut(s)	13, 98, 515
BcuI	ACTAGT	1 cut(s)	1447
BfaI	CTAG	8 cut(s)	39, 171, 377, 845, 1076, 1290, 1448, 1496
BfmI	CTRYAG	1 cut(s)	577
BfoI	RGCGCY	3 cut(s)	8, 249, 270
BfrI	CTTAAG	1 cut(s)	1204
BfuI	GTATCC	2 cut(s)	471, 883
BglI	GCCNNNNNGGC	1 cut(s)	257
BisI	GCNGC	8 cut(s)	175, 183, 249, 1419, 1428, 1653, 2079, 2154
BlsI	GCNGC	8 cut(s)	176, 184, 250, 1420, 1429, 1654, 2080, 2155
Bme1390I	CCNGG	4 cut(s)	142, 318, 1488, 1994
BmeT110I	CYCGRG	1 cut(s)	2118
BmgT120I	GGNCC	1 cut(s)	315
BmiI	GGNNCC	3 cut(s)	897, 1114, 2037
BmrFI	CCNGG	4 cut(s)	142, 318, 1488, 1994
BmsI	GCATC	7 cut(s)	465, 502, 1045, 1759, 1973, 1995, 2065
BmtI	GCTAGC	2 cut(s)	848, 1499
BpiI	GAAGAC	2 cut(s)	344, 1470
BpmI	CTGGAG	2 cut(s)	1025, 1581
Bpu10I	CCTNAGC	1 cut(s)	2082
Bpu14I	TTCGAA	1 cut(s)	190
BpuEI	CTTGAG	2 cut(s)	384, 1397
BpuMI	CCSGG	1 cut(s)	318
BsaAI	YACGTR	1 cut(s)	1097
BsaBI	GATNNNNATC	3 cut(s)	492, 894, 1757
BsaJI	CCNNGG	7 cut(s)	140, 228, 255, 472, 1162, 1486, 1993
BsaWI	WCCGGW	2 cut(s)	338, 2130
BsaXI	ACNNNNNCTCC	4 cut(s)	95, 125, 512, 542
Bsc4I	CCNNNNNNNGG	3 cut(s)	146, 147, 1616
Bse1I	ACTGG	3 cut(s)	441, 1042, 1383
Bse8I	GATNNNNATC	3 cut(s)	492, 894, 1757
BseAI	TCCGGA	1 cut(s)	2130
BseBI	CCWGG	3 cut(s)	142, 1488, 1994
BseDI	CCNNGG	7 cut(s)	140, 228, 255, 472, 1162, 1486, 1993
BseGI	GGATG	4 cut(s)	493, 1366, 1791, 2077
BseJI	GATNNNNATC	3 cut(s)	492, 894, 1757
BseLI	CCNNNNNNNGG	3 cut(s)	146, 147, 1616
BseMII	CTCAG	5 cut(s)	8, 14, 474, 624, 2073
BseNI	ACTGG	3 cut(s)	441, 1042, 1383
BseX3I	CGGCCG	1 cut(s)	469
BseXI	GCAGC	8 cut(s)	161, 169, 235, 1414, 1430, 1664, 2065, 2140
BsgI	GTGCAG	1 cut(s)	2139
Bsh1285I	CGRYCG	1 cut(s)	472
BshFI	GGCC	6 cut(s)	68, 316, 440, 471, 1278, 1992
BsiEI	CGRYCG	1 cut(s)	472
BsiHKAI	GWGCWC	2 cut(s)	31, 1789
BsiHKCI	CYCGRG	1 cut(s)	2118
BsiSI	CCGG	3 cut(s)	318, 339, 2131
BslFI	GGGAC	2 cut(s)	347, 1658
BslI	CCNNNNNNNGG	3 cut(s)	146, 147, 1616
BsmAI	GTCTC	3 cut(s)	13, 98, 515
BsmFI	GGGAC	2 cut(s)	347, 1658
BsmI	GAATGC	1 cut(s)	1974
BsnI	GGCC	6 cut(s)	68, 316, 440, 471, 1278, 1992
BsoBI	CYCGRG	1 cut(s)	2118
Bsp119I	TTCGAA	1 cut(s)	190
Bsp1286I	GDGCHC	2 cut(s)	31, 1789
Bsp13I	TCCGGA	1 cut(s)	2130
Bsp143I	GATC	6 cut(s)	271, 870, 895, 2035, 2163, 2173
BspACI	CCGC	1 cut(s)	185
BspANI	GGCC	6 cut(s)	68, 316, 440, 471, 1278, 1992
BspCNI	CTCAG	5 cut(s)	9, 15, 473, 625, 2074
BspEI	TCCGGA	1 cut(s)	2130
BspHI	TCATGA	2 cut(s)	867, 985
BspLI	GGNNCC	3 cut(s)	897, 1114, 2037
BspOI	GCTAGC	2 cut(s)	848, 1499
BspPI	GGATC	5 cut(s)	890, 903, 2030, 2043, 2181
BspT104I	TTCGAA	1 cut(s)	190
BspTI	CTTAAG	1 cut(s)	1204
BsrI	ACTGG	3 cut(s)	441, 1042, 1383
BssECI	CCNNGG	7 cut(s)	140, 228, 255, 472, 1162, 1486, 1993
BssMI	GATC	6 cut(s)	271, 870, 895, 2035, 2163, 2173
BssSI	CACGAG	1 cut(s)	690
BssT1I	CCWWGG	1 cut(s)	1162
Bst2BI	CACGAG	1 cut(s)	690
Bst2UI	CCWGG	3 cut(s)	142, 1488, 1994
Bst4CI	ACNGT	3 cut(s)	526, 535, 1072
Bst6I	CTCTTC	5 cut(s)	112, 122, 987, 1309, 1338
BstAFI	CTTAAG	1 cut(s)	1204
BstBAI	YACGTR	1 cut(s)	1097
BstBI	TTCGAA	1 cut(s)	190
BstC8I	GCNNGC	5 cut(s)	543, 846, 1497, 1902, 2158
BstDEI	CTNAG	8 cut(s)	17, 23, 295, 460, 633, 878, 1613, 2082
BstDSI	CCRYGG	2 cut(s)	255, 472
BstEII	GGTNACC	1 cut(s)	223
BstF5I	GGATG	4 cut(s)	493, 1366, 1791, 2077
BstH2I	RGCGCY	3 cut(s)	8, 249, 270
BstHHI	GCGC	3 cut(s)	7, 248, 269
BstKTI	GATC	6 cut(s)	274, 873, 898, 2038, 2166, 2176
BstMAI	GTCTC	3 cut(s)	13, 98, 515
BstMBI	GATC	6 cut(s)	271, 870, 895, 2035, 2163, 2173
BstMCI	CGRYCG	1 cut(s)	472
BstMWI	GCNNNNNNNGC	8 cut(s)	257, 266, 1415, 1424, 1427, 1436, 2084, 2166
BstNI	CCWGG	3 cut(s)	142, 1488, 1994
BstNSI	RCATGY	3 cut(s)	545, 1220, 1239
BstPI	GGTNACC	1 cut(s)	223
BstSCI	CCNGG	4 cut(s)	140, 316, 1486, 1992
BstSFI	CTRYAG	1 cut(s)	577
BstSNI	TACGTA	1 cut(s)	1097
BstV1I	GCAGC	8 cut(s)	161, 169, 235, 1414, 1430, 1664, 2065, 2140
BstV2I	GAAGAC	2 cut(s)	344, 1470
BstX2I	RGATCY	2 cut(s)	895, 2035
BstXI	CCANNNNNNTGG	1 cut(s)	1124
BstYI	RGATCY	2 cut(s)	895, 2035
BstZI	CGGCCG	1 cut(s)	469
BsuI	GTATCC	2 cut(s)	471, 883
BsuRI	GGCC	6 cut(s)	68, 316, 440, 471, 1278, 1992
BtgI	CCRYGG	2 cut(s)	255, 472
BtgZI	GCGATG	1 cut(s)	1670
BtsCI	GGATG	4 cut(s)	493, 1366, 1791, 2077
BtsIMutI	CAGTG	2 cut(s)	1049, 1077
Cac8I	GCNNGC	5 cut(s)	543, 846, 1497, 1902, 2158
CciI	TCATGA	2 cut(s)	867, 985
CfoI	GCGC	3 cut(s)	7, 248, 269
Cfr13I	GGNCC	1 cut(s)	315
CseI	GACGC	2 cut(s)	1599, 1931
Csp6I	GTAC	2 cut(s)	1098, 1376
CspCI	CAANNNNNGTGG	4 cut(s)	454, 489, 541, 576
CviQI	GTAC	2 cut(s)	1098, 1376
DdeI	CTNAG	8 cut(s)	17, 23, 295, 460, 633, 878, 1613, 2082
DpnI	GATC	6 cut(s)	273, 872, 897, 2037, 2165, 2175
DpnII	GATC	6 cut(s)	271, 870, 895, 2035, 2163, 2173
DraI	TTTAAA	4 cut(s)	742, 793, 855, 1726
DrdI	GACNNNNNNGTC	1 cut(s)	449
DseDI	GACNNNNNNGTC	1 cut(s)	449
EaeI	YGGCCR	2 cut(s)	438, 469
EagI	CGGCCG	1 cut(s)	469
Eam1104I	CTCTTC	5 cut(s)	112, 122, 987, 1309, 1338
EarI	CTCTTC	5 cut(s)	112, 122, 987, 1309, 1338
Ecl136II	GAGCTC	1 cut(s)	29
EclXI	CGGCCG	1 cut(s)	469
Eco105I	TACGTA	1 cut(s)	1097
Eco130I	CCWWGG	1 cut(s)	1162
Eco147I	AGGCCT	1 cut(s)	1992
Eco24I	GRGCYC	1 cut(s)	31
Eco32I	GATATC	1 cut(s)	1826
Eco47III	AGCGCT	1 cut(s)	247
Eco52I	CGGCCG	1 cut(s)	469
Eco53kI	GAGCTC	1 cut(s)	29
Eco57I	CTGAAG	4 cut(s)	106, 1542, 1804, 1925
Eco88I	CYCGRG	1 cut(s)	2118
Eco91I	GGTNACC	1 cut(s)	223
EcoICRI	GAGCTC	1 cut(s)	29
EcoO65I	GGTNACC	1 cut(s)	223
EcoRII	CCWGG	3 cut(s)	140, 1486, 1992
EcoRV	GATATC	1 cut(s)	1826
EcoT14I	CCWWGG	1 cut(s)	1162
EcoT22I	ATGCAT	3 cut(s)	495, 1060, 1966
EcoT38I	GRGCYC	1 cut(s)	31
ErhI	CCWWGG	1 cut(s)	1162
FalI	AAGNNNNNCTT	2 cut(s)	1329, 1361
FaqI	GGGAC	2 cut(s)	347, 1658
FbaI	TGATCA	3 cut(s)	271, 870, 2163
Fnu4HI	GCNGC	8 cut(s)	175, 183, 249, 1419, 1428, 1653, 2079, 2154
FokI	GGATG	4 cut(s)	480, 1353, 1778, 2084
FriOI	GRGCYC	1 cut(s)	31
Fsp4HI	GCNGC	8 cut(s)	175, 183, 249, 1419, 1428, 1653, 2079, 2154
FspBI	CTAG	8 cut(s)	39, 171, 377, 845, 1076, 1290, 1448, 1496
GlaI	GCGC	3 cut(s)	6, 247, 268
GluI	GCNGC	8 cut(s)	175, 183, 249, 1419, 1428, 1653, 2079, 2154
GsuI	CTGGAG	2 cut(s)	1025, 1581
HaeII	RGCGCY	3 cut(s)	8, 249, 270
HaeIII	GGCC	6 cut(s)	68, 316, 440, 471, 1278, 1992
HapII	CCGG	3 cut(s)	318, 339, 2131
HgaI	GACGC	2 cut(s)	1599, 1931
HhaI	GCGC	3 cut(s)	7, 248, 269
Hin6I	GCGC	3 cut(s)	5, 246, 267
HinP1I	GCGC	3 cut(s)	5, 246, 267
HincII	GTYRAC	2 cut(s)	531, 1143
HindII	GTYRAC	2 cut(s)	531, 1143
HindIII	AAGCTT	3 cut(s)	1134, 1178, 1937
HinfI	GANTC	8 cut(s)	277, 762, 768, 1020, 1382, 1526, 1553, 1687
HpaI	GTTAAC	1 cut(s)	1143
HpaII	CCGG	3 cut(s)	318, 339, 2131
HphI	GGTGA	2 cut(s)	1223, 1720
Hpy166II	GTNNAC	2 cut(s)	531, 1143
Hpy8I	GTNNAC	2 cut(s)	531, 1143
HpyAV	CCTTC	9 cut(s)	154, 284, 1220, 1268, 1541, 1705, 1960, 1965, 2128
HpyCH4III	ACNGT	3 cut(s)	526, 535, 1072
HpyCH4IV	ACGT	2 cut(s)	165, 1096
HpyCH4V	TGCA	9 cut(s)	493, 541, 932, 1058, 1430, 1571, 1854, 1964, 2156
HpyF10VI	GCNNNNNNNGC	8 cut(s)	257, 266, 1415, 1424, 1427, 1436, 2084, 2166
HpyF3I	CTNAG	8 cut(s)	17, 23, 295, 460, 633, 878, 1613, 2082
HpySE526I	ACGT	2 cut(s)	165, 1096
HspAI	GCGC	3 cut(s)	5, 246, 267
Kpn2I	TCCGGA	1 cut(s)	2130
Ksp22I	TGATCA	3 cut(s)	271, 870, 2163
KspAI	GTTAAC	1 cut(s)	1143
Kzo9I	GATC	6 cut(s)	271, 870, 895, 2035, 2163, 2173
LmnI	GCTCC	1 cut(s)	841
Lsp1109I	GCAGC	8 cut(s)	161, 169, 235, 1414, 1430, 1664, 2065, 2140
LweI	GCATC	7 cut(s)	465, 502, 1045, 1759, 1973, 1995, 2065
MaeI	CTAG	8 cut(s)	39, 171, 377, 845, 1076, 1290, 1448, 1496
MaeII	ACGT	2 cut(s)	165, 1096
MaeIII	GTNAC	7 cut(s)	81, 223, 499, 646, 703, 1362, 1742
MalI	GATC	6 cut(s)	273, 872, 897, 2037, 2165, 2175
MboI	GATC	6 cut(s)	271, 870, 895, 2035, 2163, 2173
MfeI	CAATTG	2 cut(s)	732, 822
MflI	RGATCY	2 cut(s)	895, 2035
MhlI	GDGCHC	2 cut(s)	31, 1789
Mph1103I	ATGCAT	3 cut(s)	495, 1060, 1966
MroI	TCCGGA	1 cut(s)	2130
MroXI	GAANNNNTTC	2 cut(s)	1674, 2142
MslI	CAYNNNNRTG	5 cut(s)	88, 546, 1122, 1371, 2017
MspA1I	CMGCKG	1 cut(s)	1559
MspCI	CTTAAG	1 cut(s)	1204
MspI	CCGG	3 cut(s)	318, 339, 2131
MspR9I	CCNGG	4 cut(s)	142, 318, 1488, 1994
MunI	CAATTG	2 cut(s)	732, 822
Mva1269I	GAATGC	1 cut(s)	1974
MvaI	CCWGG	3 cut(s)	142, 1488, 1994
MwoI	GCNNNNNNNGC	8 cut(s)	257, 266, 1415, 1424, 1427, 1436, 2084, 2166
NciI	CCSGG	1 cut(s)	318
NdeII	GATC	6 cut(s)	271, 870, 895, 2035, 2163, 2173
NheI	GCTAGC	2 cut(s)	844, 1495
NlaIV	GGNNCC	3 cut(s)	897, 1114, 2037
NmuCI	GTSAC	2 cut(s)	81, 703
NsiI	ATGCAT	3 cut(s)	495, 1060, 1966
NspI	RCATGY	3 cut(s)	545, 1220, 1239
NspV	TTCGAA	1 cut(s)	190
PaeI	GCATGC	1 cut(s)	545
PagI	TCATGA	2 cut(s)	867, 985
PceI	AGGCCT	1 cut(s)	1992
PciI	ACATGT	1 cut(s)	1235
PctI	GAATGC	1 cut(s)	1974
PdmI	GAANNNNTTC	2 cut(s)	1674, 2142
PfeI	GAWTC	8 cut(s)	277, 762, 768, 1020, 1382, 1526, 1553, 1687
PkrI	GCNGC	8 cut(s)	176, 184, 250, 1420, 1429, 1654, 2080, 2155
Ppu21I	YACGTR	1 cut(s)	1097
PscI	ACATGT	1 cut(s)	1235
PshBI	ATTAAT	1 cut(s)	1029
Psp124BI	GAGCTC	1 cut(s)	31
Psp6I	CCWGG	3 cut(s)	140, 1486, 1992
PspEI	GGTNACC	1 cut(s)	223
PspGI	CCWGG	3 cut(s)	140, 1486, 1992
PspN4I	GGNNCC	3 cut(s)	897, 1114, 2037
PspPI	GGNCC	1 cut(s)	315
PsuI	RGATCY	2 cut(s)	895, 2035
PvuII	CAGCTG	1 cut(s)	1559
RsaI	GTAC	2 cut(s)	1099, 1377
RsaNI	GTAC	2 cut(s)	1098, 1376
RseI	CAYNNNNRTG	5 cut(s)	88, 546, 1122, 1371, 2017
SacI	GAGCTC	1 cut(s)	31
SatI	GCNGC	8 cut(s)	175, 183, 249, 1419, 1428, 1653, 2079, 2154
Sau3AI	GATC	6 cut(s)	271, 870, 895, 2035, 2163, 2173
Sau96I	GGNCC	1 cut(s)	315
ScrFI	CCNGG	4 cut(s)	142, 318, 1488, 1994
SduI	GDGCHC	2 cut(s)	31, 1789
SfaNI	GCATC	7 cut(s)	465, 502, 1045, 1759, 1973, 1995, 2065
SfcI	CTRYAG	1 cut(s)	577
SfuI	TTCGAA	1 cut(s)	190
SmiMI	CAYNNNNRTG	5 cut(s)	88, 546, 1122, 1371, 2017
SmlI	CTYRAG	3 cut(s)	399, 1204, 1412
SmoI	CTYRAG	3 cut(s)	399, 1204, 1412
SnaBI	TACGTA	1 cut(s)	1097
SpeI	ACTAGT	1 cut(s)	1447
SphI	GCATGC	1 cut(s)	545
SseBI	AGGCCT	1 cut(s)	1992
SsiI	CCGC	1 cut(s)	185
SspMI	CTAG	8 cut(s)	39, 171, 377, 845, 1076, 1290, 1448, 1496
SstI	GAGCTC	1 cut(s)	31
StuI	AGGCCT	1 cut(s)	1992
StyD4I	CCNGG	4 cut(s)	140, 316, 1486, 1992
StyI	CCWWGG	1 cut(s)	1162
TaaI	ACNGT	3 cut(s)	526, 535, 1072
TaiI	ACGT	2 cut(s)	168, 1099
TaqI	TCGA	2 cut(s)	154, 190
TatI	WGTACW	1 cut(s)	1375
TfiI	GAWTC	8 cut(s)	277, 762, 768, 1020, 1382, 1526, 1553, 1687
TscAI	CASTG	2 cut(s)	1049, 1077
TseFI	GTSAC	2 cut(s)	81, 703
TseI	GCWGC	8 cut(s)	174, 182, 248, 1418, 1427, 1652, 2078, 2153
Tsp45I	GTSAC	2 cut(s)	81, 703
TspDTI	ATGAA	9 cut(s)	52, 67, 196, 344, 384, 933, 974, 1002, 1683
TspGWI	ACGGA	2 cut(s)	908, 1122
TspRI	CASTG	2 cut(s)	1049, 1077
Vha464I	CTTAAG	1 cut(s)	1204
VspI	ATTAAT	1 cut(s)	1029
XapI	RAATTY	3 cut(s)	737, 1623, 1674
XceI	RCATGY	3 cut(s)	545, 1220, 1239
XcmI	CCANNNNNNNNNTGG	1 cut(s)	824
XmnI	GAANNNNTTC	2 cut(s)	1674, 2142
XspI	CTAG	8 cut(s)	39, 171, 377, 845, 1076, 1290, 1448, 1496
Zsp2I	ATGCAT	3 cut(s)	495, 1060, 1966

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.