Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0006623

Physical Location & Seq

Genomic Coordinates Forward (+)

38980343 .. 38993607

Gene Structure 13265 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018881

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2193 bp

ATGGCGCTTCGTCTTCTGAGAGCTCATTCTCCTAGTCCTCCTTATCTTCTTCGCTTCATGGCCTTACGCTTCATCTCACACTCATGTCTCCACCCTCACTCTTCCTCCCTCTTCTTCAGCCCACCGCCACCACCTCTTGGTGGTCACAGGATTGGGCTTCGATTGAAGGACGTTCCTAGCTGCTGTGCGGTTCGAAATGTGAGAGCTTTCATGTCGTCCAGCTCGGTAACCGAGGCTTTTCAAGGAAGCTCTGCCTCCACGGCTTATGGCGCTGATCAGATTCAGGTTTTAGAAGGCTTAGAGGCTGTTAGAAAGAGGCCCGGAATGTATATTGGGGACACCGGATTTCGTGGTCTTCATCATCTGGTTTATGAAATACTAGACAATGCTGTTGATGAGGCTCAAGGCGGATATGCTTCAAACGTTGAGGTCATTTTACTGGCCGACAATTCTGTCAGCATCACAGACAACGGCCGTGGGATACCTACTGATATGCATCCTGTAACCGAGAAATCTACACTGGAGACTGTGTTGACGGTTTTGCATGCTGGTGGCAAGTTTGGTGGTTCAAGTAGTGGCTATAGTGTGTCTGGTGGATTACATGGTGTGGGTTTATCAGTCGTTAATGCCTTGTCTGAGGCATTAGAAGTTACTATTTGGCGTGATGGAATGGAATACCAGCAAAAGTATTCTCGTGGAAAGCCTGTGACAACTCTATCTTGTCATGTGCTTCCAATTGAATTTAAAGATAGACAAGGAACGCGAATCAGATTCTTGCCTGACAAACAAGTATTTAAAACTCTTAATCAGTTCGACTACCACACAATTGCTGGAAGAATTAGGGAGCTAGCTTTTTTAAACCCTAATCTCATGATCACTCTTAGACGAGAGGATATGGATCCAGAGAAAATCCAGCACAATGAATACTTCTATGCAGGGGGTTTGGTTGAATATGTTAGATGGCTAAATACTGATAAGAAACCTCTTCATGAAGTGGTGGGTTTCAGAAAAGAAGTAGATGGAATCACTATTAATGTAGCTCTCCAGTGGTGTTCTGATGCATATTCAGATACAGTGCTAGGGTATGCTAATAGCATACGTACTGCTGACGGAGGAACCCATATTGATGGACTGAAAGCTTCGTTAACAAGAACGCTGAATAACCTTGGGAAGAAGTCAAAGCTTATCAAGGATAAAGATATTACCTTAAGTGGTGAGCATGTAAGAGAAGGGCTGACATGTGTAGTTTCAGTCAAAGTTCCCAATCCAGAGTTTGAAGGCCAGACAAAGACTAGGTTGGGAAATCCAGAGGTGCGAAGAGTGGTTGATAATTCTCTTCAAGAGTATCTTACAGAATACTTGGAGTTACATCCTGATGTACTGGATTCAATCCTTTGCAAGTCCTTAAATGCTCTCAAGGCAGCTCTGGCTGCAAAGAGGGCAAGGGAACTAGTCAGACAAAAGAGTGTTTTGCGTTCATCGTCTCTCCCAGGAAAGCTAGCTGATTGCTCATCAACCAATCCTGAAGAATCTGAAATCTTTATAGTTGAAGGAGATTCAGCTGGAGGAAGTGCAAAGCAAGGTCGTGATAGACGCTTTCAGGCAATTCTTCCACTAAGGGGTAAAATTCTGAACATTGAAAGGAGGGACGAGGCAGCAATGTATAAAAATGAAGAAATTCAAAATCTGATTCTTGGACTTGGACTTGGGGTGAAGGGAGAGGACTTTAAAAAGGAAGCTCTGCGTTACCATAAGATTATCATCTTAACTGATGCTGATGTAGATGGTGCTCACATCCGAACTCTGCTACTGACATTTTTCTTCAGATATCAGAGAGCCTTATTTGATGAGGGTTGCATATATGTTGGTGTTCCACCCTTTTACAAGGTTGAAAGGGGAAAGCAAGCATATTATTGCTATGATGACGCTGAACTTAAAAAGCTTCAGAGTTCCTTCCCTTCAAATGCATCATACAACATTCAAAGGTTCAAAGGCCTAGGAGAAATGATGCCCACACAATTATGGGAAACAACAATGGATCCTCAGAAAAGGCTGCTGAAACAATTAAGTGTGGATGATGCTGCTGAGGCTAACTTTGTCTTTTCCTCCCTTATGGGTTCTCGGGTGGATTTCCGGAAGGAGCTCATTCAAAATGCTGCACGCATGATCAACTTGGATCAGTTAGATATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003916 GO:0003918 GO:0005575 GO:0006139 GO:0006259 GO:0006265 GO:0006725 GO:0006807 GO:0006996 GO:0007059 GO:0008094 GO:0008150 GO:0008152 GO:0009295 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0017111 GO:0034641 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0051276 GO:0061505 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_gyraseB DNA_gyraseB_C HATPase_c Toprim

Protein Analysis

731

Amino Acids

81.37

Weight (kDa)

8.16

Isoelectric Point (pI)

42.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HATPase_c	PF02518	118 - 260	6.1e-17	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
DNA_gyraseB	PF00204	314 - 483	1.6e-54	DNA gyrase B
Toprim	PF01751	512 - 612	5.4e-18	Toprim domain
DNA_gyraseB_C	PF00986	656 - 717	4.4e-25	DNA gyrase B subunit, carboxyl terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10270 AT3G10270 AT5G04130
fragaria_vesca	FvH4_6g25390 FvH4_6g25390 FvH4_6g25390
malus_domestica	MD12G1095800.v1.1 MD14G1090600.v1.1
prunus_persica	Prupe.7G033800_v2.0.a1
pyrus_communis	pycom12g08270
rosa_chinensis	RchiOBHm_Chr2g0125831
rosa_laevigata	RLG00000018881
rosa_multiflora	Rmu_co8103446.1_g000001 Rmu_sc0009079.1_g000002
rosa_roxburghii	Rroxscaffold_2G00117620 Rroxscaffold_2G00117680 Rroxscaffold_2G00125340 Rroxscaffold_2G00125370 Rroxscaffold_3G00238600 Rroxscaffold_4G00318520 Rroxscaffold_4G00324150 Rroxscaffold_5G00337460
rosa_rugosa	Rorug02G0259400
rosa_samantha	Rh2AG317700 Rh2BG326100
rosa_wichuraiana	Rw2G025700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	452
AccB7I	CCANNNNNTGG	1 cut(s)	137
AccII	CGCG	1 cut(s)	763
AccIII	TCCGGA	1 cut(s)	2133
AciI	CCGC	3 cut(s)	125, 188, 408
AclI	AACGTT	1 cut(s)	423
AclWI	GGATC	5 cut(s)	893, 906, 2033, 2046, 2184
AcoI	YGGCCR	2 cut(s)	441, 472
AcsI	RAATTY	3 cut(s)	740, 1626, 1677
AcuI	CTGAAG	4 cut(s)	100, 1545, 1807, 1928
AfaI	GTAC	2 cut(s)	1102, 1380
AfiI	CCNNNNNNNGG	3 cut(s)	137, 140, 1619
AflII	CTTAAG	1 cut(s)	1207
AflIII	ACRYGT	1 cut(s)	1238
AhlI	ACTAGT	1 cut(s)	1450
AjnI	CCWGG	1 cut(s)	1489
Alw21I	GWGCWC	3 cut(s)	25, 1792, 2145
Alw26I	GTCTC	3 cut(s)	92, 518, 1488
AlwI	GGATC	5 cut(s)	893, 906, 2033, 2046, 2184
Ama87I	CYCGRG	1 cut(s)	2121
Aor13HI	TCCGGA	1 cut(s)	2133
AoxI	GGCC	6 cut(s)	60, 317, 441, 472, 1279, 1993
ApeKI	GCWGC	7 cut(s)	180, 1421, 1430, 1655, 2053, 2081, 2156
ApoI	RAATTY	3 cut(s)	740, 1626, 1677
AseI	ATTAAT	1 cut(s)	1032
Asp700I	GAANNNNTTC	1 cut(s)	1677
AspA2I	CCTAGG	1 cut(s)	1996
AspLEI	GCGC	2 cut(s)	7, 272
AspS9I	GGNCC	1 cut(s)	318
AsuC2I	CCSGG	1 cut(s)	321
AsuHPI	GGTGA	2 cut(s)	1226, 1723
AsuII	TTCGAA	1 cut(s)	193
AsuNHI	GCTAGC	2 cut(s)	847, 1498
AvaI	CYCGRG	1 cut(s)	2121
AvrII	CCTAGG	1 cut(s)	1996
BamHI	GGATCC	2 cut(s)	898, 2038
BanII	GRGCYC	2 cut(s)	25, 2145
BauI	CACGAG	1 cut(s)	693
BbsI	GAAGAC	2 cut(s)	5, 347
Bbv12I	GWGCWC	3 cut(s)	25, 1792, 2145
BbvCI	CCTCAGC	1 cut(s)	2085
BbvI	GCAGC	7 cut(s)	167, 1417, 1433, 1667, 2040, 2068, 2143
BccI	CCATC	5 cut(s)	659, 954, 1013, 1121, 1778
BceAI	ACGGC	3 cut(s)	276, 459, 487
BciT130I	CCWGG	1 cut(s)	1491
BciVI	GTATCC	1 cut(s)	474
BclI	TGATCA	3 cut(s)	274, 873, 2166
BcnI	CCSGG	1 cut(s)	321
BcoDI	GTCTC	3 cut(s)	92, 518, 1488
BcuI	ACTAGT	1 cut(s)	1450
BfaI	CTAG	9 cut(s)	33, 177, 380, 848, 1079, 1293, 1451, 1499, 1997
BfmI	CTRYAG	1 cut(s)	580
BfoI	RGCGCY	2 cut(s)	8, 273
BfrI	CTTAAG	1 cut(s)	1207
BfuI	GTATCC	1 cut(s)	474
BglI	GCCNNNNNGGC	1 cut(s)	260
BisI	GCNGC	7 cut(s)	181, 1422, 1431, 1656, 2054, 2082, 2157
BlnI	CCTAGG	1 cut(s)	1996
BlsI	GCNGC	7 cut(s)	182, 1423, 1432, 1657, 2055, 2083, 2158
Bme1390I	CCNGG	2 cut(s)	321, 1491
BmeT110I	CYCGRG	1 cut(s)	2121
BmgT120I	GGNCC	1 cut(s)	318
BmiI	GGNNCC	3 cut(s)	900, 1117, 2040
BmrFI	CCNGG	2 cut(s)	321, 1491
BmsI	GCATC	7 cut(s)	468, 505, 1048, 1762, 1976, 1998, 2068
BmtI	GCTAGC	2 cut(s)	851, 1502
BpiI	GAAGAC	2 cut(s)	5, 347
BpmI	CTGGAG	3 cut(s)	542, 1028, 1584
Bpu10I	CCTNAGC	1 cut(s)	2085
Bpu14I	TTCGAA	1 cut(s)	193
BpuEI	CTTGAG	2 cut(s)	387, 1400
BpuMI	CCSGG	1 cut(s)	321
BsaAI	YACGTR	1 cut(s)	1100
BsaBI	GATNNNNATC	3 cut(s)	495, 897, 1760
BsaJI	CCNNGG	6 cut(s)	231, 258, 475, 1165, 1489, 1996
BsaWI	WCCGGW	2 cut(s)	341, 2133
BsaXI	ACNNNNNCTCC	4 cut(s)	89, 119, 515, 545
Bsc4I	CCNNNNNNNGG	3 cut(s)	137, 140, 1619
Bse1I	ACTGG	4 cut(s)	444, 525, 1045, 1386
Bse3DI	GCAATG	1 cut(s)	1665
Bse8I	GATNNNNATC	3 cut(s)	495, 897, 1760
BseAI	TCCGGA	1 cut(s)	2133
BseBI	CCWGG	1 cut(s)	1491
BseDI	CCNNGG	6 cut(s)	231, 258, 475, 1165, 1489, 1996
BseGI	GGATG	4 cut(s)	496, 1369, 1794, 2080
BseJI	GATNNNNATC	3 cut(s)	495, 897, 1760
BseLI	CCNNNNNNNGG	3 cut(s)	137, 140, 1619
BseMI	GCAATG	1 cut(s)	1665
BseMII	CTCAG	4 cut(s)	8, 627, 2057, 2076
BseNI	ACTGG	4 cut(s)	444, 525, 1045, 1386
BseX3I	CGGCCG	1 cut(s)	472
BseXI	GCAGC	7 cut(s)	167, 1417, 1433, 1667, 2040, 2068, 2143
BsgI	GTGCAG	1 cut(s)	2142
Bsh1236I	CGCG	1 cut(s)	763
Bsh1285I	CGRYCG	1 cut(s)	475
BshFI	GGCC	6 cut(s)	62, 319, 443, 474, 1281, 1995
BsiEI	CGRYCG	1 cut(s)	475
BsiHKAI	GWGCWC	3 cut(s)	25, 1792, 2145
BsiHKCI	CYCGRG	1 cut(s)	2121
BsiSI	CCGG	3 cut(s)	321, 342, 2134
BslFI	GGGAC	2 cut(s)	350, 1661
BslI	CCNNNNNNNGG	3 cut(s)	137, 140, 1619
BsmAI	GTCTC	3 cut(s)	92, 518, 1488
BsmBI	CGTCTC	1 cut(s)	1488
BsmFI	GGGAC	2 cut(s)	350, 1661
BsnI	GGCC	6 cut(s)	62, 319, 443, 474, 1281, 1995
BsoBI	CYCGRG	1 cut(s)	2121
Bsp119I	TTCGAA	1 cut(s)	193
Bsp1286I	GDGCHC	3 cut(s)	25, 1792, 2145
Bsp13I	TCCGGA	1 cut(s)	2133
Bsp143I	GATC	6 cut(s)	274, 873, 898, 2038, 2166, 2176
BspACI	CCGC	3 cut(s)	125, 188, 408
BspANI	GGCC	6 cut(s)	62, 319, 443, 474, 1281, 1995
BspCNI	CTCAG	4 cut(s)	9, 628, 2056, 2077
BspEI	TCCGGA	1 cut(s)	2133
BspFNI	CGCG	1 cut(s)	763
BspHI	TCATGA	2 cut(s)	870, 988
BspLI	GGNNCC	3 cut(s)	900, 1117, 2040
BspOI	GCTAGC	2 cut(s)	851, 1502
BspPI	GGATC	5 cut(s)	893, 906, 2033, 2046, 2184
BspT104I	TTCGAA	1 cut(s)	193
BspTI	CTTAAG	1 cut(s)	1207
BsrDI	GCAATG	1 cut(s)	1665
BsrI	ACTGG	4 cut(s)	444, 525, 1045, 1386
BssECI	CCNNGG	6 cut(s)	231, 258, 475, 1165, 1489, 1996
BssMI	GATC	6 cut(s)	274, 873, 898, 2038, 2166, 2176
BssSI	CACGAG	1 cut(s)	693
BssT1I	CCWWGG	2 cut(s)	1165, 1996
Bst2BI	CACGAG	1 cut(s)	693
Bst2UI	CCWGG	1 cut(s)	1491
Bst4CI	ACNGT	3 cut(s)	529, 538, 1075
Bst6I	CTCTTC	5 cut(s)	106, 116, 990, 1312, 1341
BstAFI	CTTAAG	1 cut(s)	1207
BstBAI	YACGTR	1 cut(s)	1100
BstBI	TTCGAA	1 cut(s)	193
BstC8I	GCNNGC	5 cut(s)	546, 849, 1500, 1905, 2161
BstDEI	CTNAG	7 cut(s)	17, 298, 636, 881, 1616, 2043, 2085
BstDSI	CCRYGG	2 cut(s)	258, 475
BstEII	GGTNACC	1 cut(s)	226
BstF5I	GGATG	4 cut(s)	496, 1369, 1794, 2080
BstFNI	CGCG	1 cut(s)	763
BstH2I	RGCGCY	2 cut(s)	8, 273
BstHHI	GCGC	2 cut(s)	7, 272
BstKTI	GATC	6 cut(s)	277, 876, 901, 2041, 2169, 2179
BstMAI	GTCTC	3 cut(s)	92, 518, 1488
BstMBI	GATC	6 cut(s)	274, 873, 898, 2038, 2166, 2176
BstMCI	CGRYCG	1 cut(s)	475
BstMWI	GCNNNNNNNGC	7 cut(s)	260, 269, 1418, 1427, 1430, 1439, 2087
BstNI	CCWGG	1 cut(s)	1491
BstNSI	RCATGY	3 cut(s)	548, 1223, 1242
BstPI	GGTNACC	1 cut(s)	226
BstSCI	CCNGG	2 cut(s)	319, 1489
BstSFI	CTRYAG	1 cut(s)	580
BstSNI	TACGTA	1 cut(s)	1100
BstUI	CGCG	1 cut(s)	763
BstV1I	GCAGC	7 cut(s)	167, 1417, 1433, 1667, 2040, 2068, 2143
BstV2I	GAAGAC	2 cut(s)	5, 347
BstX2I	RGATCY	2 cut(s)	898, 2038
BstXI	CCANNNNNNTGG	1 cut(s)	1127
BstYI	RGATCY	2 cut(s)	898, 2038
BstZI	CGGCCG	1 cut(s)	472
BsuI	GTATCC	1 cut(s)	474
BsuRI	GGCC	6 cut(s)	62, 319, 443, 474, 1281, 1995
BtgI	CCRYGG	2 cut(s)	258, 475
BtsCI	GGATG	4 cut(s)	496, 1369, 1794, 2080
BtsIMutI	CAGTG	3 cut(s)	518, 1052, 1080
Cac8I	GCNNGC	5 cut(s)	546, 849, 1500, 1905, 2161
CciI	TCATGA	2 cut(s)	870, 988
CfoI	GCGC	2 cut(s)	7, 272
Cfr13I	GGNCC	1 cut(s)	318
CseI	GACGC	2 cut(s)	1602, 1934
Csp6I	GTAC	2 cut(s)	1101, 1379
CspCI	CAANNNNNGTGG	4 cut(s)	457, 492, 544, 579
CviQI	GTAC	2 cut(s)	1101, 1379
DdeI	CTNAG	7 cut(s)	17, 298, 636, 881, 1616, 2043, 2085
DpnI	GATC	6 cut(s)	276, 875, 900, 2040, 2168, 2178
DpnII	GATC	6 cut(s)	274, 873, 898, 2038, 2166, 2176
DraI	TTTAAA	4 cut(s)	745, 796, 858, 1729
DrdI	GACNNNNNNGTC	1 cut(s)	452
DseDI	GACNNNNNNGTC	1 cut(s)	452
EaeI	YGGCCR	2 cut(s)	441, 472
EagI	CGGCCG	1 cut(s)	472
Eam1104I	CTCTTC	5 cut(s)	106, 116, 990, 1312, 1341
EarI	CTCTTC	5 cut(s)	106, 116, 990, 1312, 1341
EciI	GGCGGA	1 cut(s)	423
Ecl136II	GAGCTC	2 cut(s)	23, 2143
EclXI	CGGCCG	1 cut(s)	472
Eco105I	TACGTA	1 cut(s)	1100
Eco130I	CCWWGG	2 cut(s)	1165, 1996
Eco147I	AGGCCT	1 cut(s)	1995
Eco24I	GRGCYC	2 cut(s)	25, 2145
Eco32I	GATATC	1 cut(s)	1829
Eco52I	CGGCCG	1 cut(s)	472
Eco53kI	GAGCTC	2 cut(s)	23, 2143
Eco57I	CTGAAG	4 cut(s)	100, 1545, 1807, 1928
Eco88I	CYCGRG	1 cut(s)	2121
Eco91I	GGTNACC	1 cut(s)	226
EcoICRI	GAGCTC	2 cut(s)	23, 2143
EcoO65I	GGTNACC	1 cut(s)	226
EcoRII	CCWGG	1 cut(s)	1489
EcoRV	GATATC	1 cut(s)	1829
EcoT14I	CCWWGG	2 cut(s)	1165, 1996
EcoT22I	ATGCAT	3 cut(s)	498, 1063, 1969
EcoT38I	GRGCYC	2 cut(s)	25, 2145
ErhI	CCWWGG	2 cut(s)	1165, 1996
Esp3I	CGTCTC	1 cut(s)	1488
FalI	AAGNNNNNCTT	2 cut(s)	1332, 1364
FaqI	GGGAC	2 cut(s)	350, 1661
FbaI	TGATCA	3 cut(s)	274, 873, 2166
Fnu4HI	GCNGC	7 cut(s)	181, 1422, 1431, 1656, 2054, 2082, 2157
FokI	GGATG	4 cut(s)	483, 1356, 1781, 2087
FriOI	GRGCYC	2 cut(s)	25, 2145
Fsp4HI	GCNGC	7 cut(s)	181, 1422, 1431, 1656, 2054, 2082, 2157
FspBI	CTAG	9 cut(s)	33, 177, 380, 848, 1079, 1293, 1451, 1499, 1997
GlaI	GCGC	2 cut(s)	6, 271
GluI	GCNGC	7 cut(s)	181, 1422, 1431, 1656, 2054, 2082, 2157
GsuI	CTGGAG	3 cut(s)	542, 1028, 1584
HaeII	RGCGCY	2 cut(s)	8, 273
HaeIII	GGCC	6 cut(s)	62, 319, 443, 474, 1281, 1995
HapII	CCGG	3 cut(s)	321, 342, 2134
HgaI	GACGC	2 cut(s)	1602, 1934
HhaI	GCGC	2 cut(s)	7, 272
Hin6I	GCGC	2 cut(s)	5, 270
HinP1I	GCGC	2 cut(s)	5, 270
HincII	GTYRAC	2 cut(s)	534, 1146
HindII	GTYRAC	2 cut(s)	534, 1146
HindIII	AAGCTT	3 cut(s)	1137, 1181, 1940
HinfI	GANTC	8 cut(s)	280, 765, 771, 1023, 1385, 1529, 1556, 1690
HpaI	GTTAAC	1 cut(s)	1146
HpaII	CCGG	3 cut(s)	321, 342, 2134
HphI	GGTGA	2 cut(s)	1226, 1723
Hpy166II	GTNNAC	2 cut(s)	534, 1146
Hpy8I	GTNNAC	2 cut(s)	534, 1146
HpyAV	CCTTC	9 cut(s)	160, 287, 1223, 1271, 1544, 1708, 1963, 1968, 2131
HpyCH4III	ACNGT	3 cut(s)	529, 538, 1075
HpyCH4IV	ACGT	3 cut(s)	171, 423, 1099
HpyF10VI	GCNNNNNNNGC	7 cut(s)	260, 269, 1418, 1427, 1430, 1439, 2087
HpyF3I	CTNAG	7 cut(s)	17, 298, 636, 881, 1616, 2043, 2085
HpySE526I	ACGT	3 cut(s)	171, 423, 1099
HspAI	GCGC	2 cut(s)	5, 270
Kpn2I	TCCGGA	1 cut(s)	2133
Ksp22I	TGATCA	3 cut(s)	274, 873, 2166
KspAI	GTTAAC	1 cut(s)	1146
Kzo9I	GATC	6 cut(s)	274, 873, 898, 2038, 2166, 2176
LmnI	GCTCC	2 cut(s)	844, 2140
Lsp1109I	GCAGC	7 cut(s)	167, 1417, 1433, 1667, 2040, 2068, 2143
LweI	GCATC	7 cut(s)	468, 505, 1048, 1762, 1976, 1998, 2068
MaeI	CTAG	9 cut(s)	33, 177, 380, 848, 1079, 1293, 1451, 1499, 1997
MaeII	ACGT	3 cut(s)	171, 423, 1099
MaeIII	GTNAC	7 cut(s)	143, 226, 502, 649, 706, 1365, 1745
MalI	GATC	6 cut(s)	276, 875, 900, 2040, 2168, 2178
MboI	GATC	6 cut(s)	274, 873, 898, 2038, 2166, 2176
MfeI	CAATTG	2 cut(s)	735, 825
MflI	RGATCY	2 cut(s)	898, 2038
MhlI	GDGCHC	3 cut(s)	25, 1792, 2145
Mph1103I	ATGCAT	3 cut(s)	498, 1063, 1969
MroI	TCCGGA	1 cut(s)	2133
MroXI	GAANNNNTTC	1 cut(s)	1677
MslI	CAYNNNNRTG	5 cut(s)	82, 549, 1125, 1374, 2020
MspA1I	CMGCKG	1 cut(s)	1562
MspCI	CTTAAG	1 cut(s)	1207
MspI	CCGG	3 cut(s)	321, 342, 2134
MspR9I	CCNGG	2 cut(s)	321, 1491
MunI	CAATTG	2 cut(s)	735, 825
MvaI	CCWGG	1 cut(s)	1491
MvnI	CGCG	1 cut(s)	763
MwoI	GCNNNNNNNGC	7 cut(s)	260, 269, 1418, 1427, 1430, 1439, 2087
NciI	CCSGG	1 cut(s)	321
NdeII	GATC	6 cut(s)	274, 873, 898, 2038, 2166, 2176
NheI	GCTAGC	2 cut(s)	847, 1498
NlaIV	GGNNCC	3 cut(s)	900, 1117, 2040
NmuCI	GTSAC	2 cut(s)	143, 706
NsiI	ATGCAT	3 cut(s)	498, 1063, 1969
NspI	RCATGY	3 cut(s)	548, 1223, 1242
NspV	TTCGAA	1 cut(s)	193
PaeI	GCATGC	1 cut(s)	548
PagI	TCATGA	2 cut(s)	870, 988
PceI	AGGCCT	1 cut(s)	1995
PciI	ACATGT	1 cut(s)	1238
PdmI	GAANNNNTTC	1 cut(s)	1677
PfeI	GAWTC	8 cut(s)	280, 765, 771, 1023, 1385, 1529, 1556, 1690
PflMI	CCANNNNNTGG	1 cut(s)	137
PkrI	GCNGC	7 cut(s)	182, 1423, 1432, 1657, 2055, 2083, 2158
Ppu21I	YACGTR	1 cut(s)	1100
PscI	ACATGT	1 cut(s)	1238
PshBI	ATTAAT	1 cut(s)	1032
Psp124BI	GAGCTC	2 cut(s)	25, 2145
Psp1406I	AACGTT	1 cut(s)	423
Psp6I	CCWGG	1 cut(s)	1489
PspEI	GGTNACC	1 cut(s)	226
PspGI	CCWGG	1 cut(s)	1489
PspN4I	GGNNCC	3 cut(s)	900, 1117, 2040
PspPI	GGNCC	1 cut(s)	318
PsuI	RGATCY	2 cut(s)	898, 2038
PvuII	CAGCTG	1 cut(s)	1562
RsaI	GTAC	2 cut(s)	1102, 1380
RsaNI	GTAC	2 cut(s)	1101, 1379
RseI	CAYNNNNRTG	5 cut(s)	82, 549, 1125, 1374, 2020
SacI	GAGCTC	2 cut(s)	25, 2145
SatI	GCNGC	7 cut(s)	181, 1422, 1431, 1656, 2054, 2082, 2157
Sau3AI	GATC	6 cut(s)	274, 873, 898, 2038, 2166, 2176
Sau96I	GGNCC	1 cut(s)	318
ScrFI	CCNGG	2 cut(s)	321, 1491
SduI	GDGCHC	3 cut(s)	25, 1792, 2145
SfaNI	GCATC	7 cut(s)	468, 505, 1048, 1762, 1976, 1998, 2068
SfcI	CTRYAG	1 cut(s)	580
SfuI	TTCGAA	1 cut(s)	193
SmiMI	CAYNNNNRTG	5 cut(s)	82, 549, 1125, 1374, 2020
SmlI	CTYRAG	3 cut(s)	402, 1207, 1415
SmoI	CTYRAG	3 cut(s)	402, 1207, 1415
SnaBI	TACGTA	1 cut(s)	1100
SpeI	ACTAGT	1 cut(s)	1450
SphI	GCATGC	1 cut(s)	548
SseBI	AGGCCT	1 cut(s)	1995
SsiI	CCGC	3 cut(s)	125, 188, 408
SspMI	CTAG	9 cut(s)	33, 177, 380, 848, 1079, 1293, 1451, 1499, 1997
SstI	GAGCTC	2 cut(s)	25, 2145
StuI	AGGCCT	1 cut(s)	1995
StyD4I	CCNGG	2 cut(s)	319, 1489
StyI	CCWWGG	2 cut(s)	1165, 1996
TaaI	ACNGT	3 cut(s)	529, 538, 1075
TaiI	ACGT	3 cut(s)	174, 426, 1102
TaqI	TCGA	3 cut(s)	160, 193, 813
TatI	WGTACW	1 cut(s)	1378
TfiI	GAWTC	8 cut(s)	280, 765, 771, 1023, 1385, 1529, 1556, 1690
TscAI	CASTG	3 cut(s)	525, 1052, 1080
TseFI	GTSAC	2 cut(s)	143, 706
TseI	GCWGC	7 cut(s)	180, 1421, 1430, 1655, 2053, 2081, 2156
Tsp45I	GTSAC	2 cut(s)	143, 706
TspGWI	ACGGA	1 cut(s)	1125
TspRI	CASTG	3 cut(s)	525, 1052, 1080
Van91I	CCANNNNNTGG	1 cut(s)	137
Vha464I	CTTAAG	1 cut(s)	1207
VspI	ATTAAT	1 cut(s)	1032
XapI	RAATTY	3 cut(s)	740, 1626, 1677
XceI	RCATGY	3 cut(s)	548, 1223, 1242
XcmI	CCANNNNNNNNNTGG	1 cut(s)	827
XmaJI	CCTAGG	1 cut(s)	1996
XmnI	GAANNNNTTC	1 cut(s)	1677
XspI	CTAG	9 cut(s)	33, 177, 380, 848, 1079, 1293, 1451, 1499, 1997
Zsp2I	ATGCAT	3 cut(s)	498, 1063, 1969

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.