Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0006623

Physical Location & Seq

Genomic Coordinates Reverse (-)

11544713 .. 11561848

Gene Structure 17136 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1090600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2226 bp

ATGGCGCTTCTTCTTCTTCGACCTCCTCCTCCTCCTCCCTCTTATCTTCGCTTCATGGCCTTACGCTTCTTCTCCCACTCCTCTCTCCGCCCTAACTTCCCGCCTCCCTCTTCTTCTCCCTCTTCGTCTCCCTCGCTTGCTCGTCCCTCTCTCCTCTTCAACCCACAGACCAGGGCGGAATTTCCATTGAAGGGCATTGGTAGCTGCTGTGTGGATCGAAATGTGGTTTCTGCAAGAGTTTTCATGTCGTCTACCACTGCAACCGAGGCTTTTCAAGGGACCACTGGCTCCAGTGCTTATGGGTCGGATCAGATTCAGGTATTGGAAGGCTTAGACCCTGTTAGGAAAAGGCCAGGAATGTATATTGGGAGCACGGGACTTCGTGGTCTTCACCATTTGGTATATGAAATATTAGATAATGCAGTTGACGAGGCTCAAGCCGGATTTGCTTCAAAGATTGAGGTCATTTTGCTCGCAGATGGTTCTGTGAGCATCACTGACAATGGTCGTGGGATTCCAACTGATATGCACCCAGCAACCAAGAAATCAGCCTTGGAGACTGTGTTGACGGTTTTACATGCTGGTGGGAAGTTTGGTGGTTCAAGTAGTGGCTATAGTGTGTCTGGTGGATTGCATGGTGTGGGTTTATCAGTCGTCAATGCCTTGTCTGAGGCTTTAGAAGTTACTGTTTGGCGTGATGGAATGGAATATCAGCAGAAGTATTGTCGTGGAAAGCCTGTGACAACTCTATCCTGTCATGTACTTCCAGTTGAATTCCAAGATCGGCATGGAACACGCATCAGATTTTGGCCTGACAAAGAAGTCTTCACAACTACTATTCAGTTTGACTACAACACGATAGCTGGACGAATTAGGGAGCTAGCTTTTCTAAACCCAAATCTCATGATCGCTCTTAGACAAGAGGATATCAATCCAGAGAAGAACCACCACAATGAATACTTCTATGTAGGGGGTTTGAATGAATATGTGAAATGGCTAAATACTGATAAGAAACCGCTTCATGATGTGGTTGGCTTTAGAAAAGAAGTAGATGGAATCACTATTGATGTAGCTCTCCAGTGGTGTTCTGATGCATATTCAGAAACAATGCTTGGGTATGCTAATAGCATACGGACTGTTGATGGAGGAACTCATATTGATGGAACAAAGGCTTCATTAACAAGAACACTAAATAGTCTTGGAAAGAAGTCAAAAGTTATCAAGGAAAAGGATATTACTTTGAGTGGTGAGCATGTGAGAGAAGGATTGACATACGTCATCTCGGTCAAAGTTCCCAATCCGGAATTTGAAGGCCAAACAAAGACTAGGTTGGGAAATCCAGAAGTGCGGAGAGTGGTTGATCAATCACTTCAAGAGTATCTTACGGAATACTTGGAGTTGCATCCAGATGTACTGGATTCAATCCTTTCAAAGTCTTTAAATGCTCTCAAGGCAGCTCTGGCTGCAAAGAGGGCAAGAGAACTAGTTAGACAAAAGAGTGTTTTGAGATCATCTTCTCTCCCAGGAAAACTATCTGATTGCTCATCAACAAATCCTGAAGAATCAGAAATCTTTATAGTTGAAGGTGATTCAGCTGGTGGAAGTGCAAAACAGGGTCGTGATAGGTGCTTTCAGGCCATTCTTCCACTGAGGGGTAAAATTCTGAACATTGAAAGGAAGGATGAGGCAGCAATGTATAAAAATGAAGAGATTCAAAATCTAATTCTTGGTCTTGGACTTGGGGTGAAGGGAGAGGACTTCAAGAAGGAGGCTCTACGTTACCATAAGATTATCATTTTAACAGATGCTGATATAGATGGTGCTCACATCCGTACTCTGCTACTGACATTTTTCTTCAGATATCAGAGAGCCTTATTTGATGAGGGCTACATATATGTTGGCGTTCCACCGCTTTACAAGGTTGAGAGGGGAAAGCAAGCCAAATATTGCTATGATGAAGCTGAACTTAAAATGCTTCAGAACTCCTTCCCTTTGAATGCGTCTTACAACATTCAGAGGTTCAAAGGCCTGGGGGAAATGATGCCCGCCCAGTTATGGGAAACAACAATGAATCCCGAGCAAAGGCTTCTTAAACGATTAGGGGTTGGGGATGCTGCTGAGGCGAACATGGTGTTTTCGTCCCTTATGGGTGCTCGGGTGGATTACCGGAAGGAGCTCATAAAGAATTCCGCGAACATGATCAACTTCGATCAGTTGGATATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003916 GO:0003918 GO:0005575 GO:0006139 GO:0006259 GO:0006265 GO:0006725 GO:0006807 GO:0006996 GO:0007059 GO:0008094 GO:0008150 GO:0008152 GO:0009295 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0017111 GO:0034641 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0051276 GO:0061505 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_gyraseB DNA_gyraseB_C HATPase_c Toprim

Protein Analysis

742

Amino Acids

82.29

Weight (kDa)

7.96

Isoelectric Point (pI)

39.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HATPase_c	PF02518	128 - 273	4.9e-17	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
DNA_gyraseB	PF00204	325 - 494	1.3e-54	DNA gyrase B
Toprim	PF01751	523 - 624	1.7e-17	Toprim domain
DNA_gyraseB_C	PF00986	667 - 727	3e-24	DNA gyrase B subunit, carboxyl terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10270 AT3G10270 AT5G04130
fragaria_vesca	FvH4_6g25390 FvH4_6g25390 FvH4_6g25390
malus_domestica	MD12G1095800.v1.1 MD14G1090600.v1.1
prunus_persica	Prupe.7G033800_v2.0.a1
pyrus_communis	pycom12g08270
rosa_chinensis	RchiOBHm_Chr2g0125831
rosa_laevigata	RLG00000018881
rosa_multiflora	Rmu_co8103446.1_g000001 Rmu_sc0009079.1_g000002
rosa_roxburghii	Rroxscaffold_2G00117620 Rroxscaffold_2G00117680 Rroxscaffold_2G00125340 Rroxscaffold_2G00125370 Rroxscaffold_3G00238600 Rroxscaffold_4G00318520 Rroxscaffold_4G00324150 Rroxscaffold_5G00337460
rosa_rugosa	Rorug02G0259400
rosa_samantha	Rh2AG317700 Rh2BG326100
rosa_wichuraiana	Rw2G025700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	384, 821
AccI	GTMKAC	1 cut(s)	251
AccII	CGCG	1 cut(s)	2192
AccIII	TCCGGA	1 cut(s)	1300
AciI	CCGC	8 cut(s)	88, 101, 176, 1016, 1348, 1910, 2046, 2190
AclWI	GGATC	2 cut(s)	222, 315
AcsI	RAATTY	5 cut(s)	179, 773, 1304, 1659, 2185
AcuI	CTGAAG	3 cut(s)	1578, 1840, 1961
AfaI	GTAC	3 cut(s)	762, 1413, 1834
AfiI	CCNNNNNNNGG	5 cut(s)	191, 1652, 2055, 2056, 2082
AhlI	ACTAGT	1 cut(s)	1483
AjnI	CCWGG	4 cut(s)	170, 352, 1522, 2028
AjuI	GAANNNNNNNTTGG	4 cut(s)	536, 568, 1095, 1127
AluBI	AGCT	9 cut(s)	204, 863, 880, 884, 1073, 1457, 1595, 1961, 2176
AluI	AGCT	9 cut(s)	204, 863, 880, 884, 1073, 1457, 1595, 1961, 2176
Alw21I	GWGCWC	4 cut(s)	374, 1825, 2155, 2178
Alw26I	GTCTC	2 cut(s)	132, 551
AlwI	GGATC	2 cut(s)	222, 315
AlwNI	CAGNNNCTG	1 cut(s)	1808
Ama87I	CYCGRG	2 cut(s)	2075, 2154
Aor13HI	TCCGGA	1 cut(s)	1300
AoxI	GGCC	6 cut(s)	57, 350, 809, 1312, 1635, 2026
ApeKI	GCWGC	5 cut(s)	204, 1454, 1463, 1688, 2114
ApoI	RAATTY	5 cut(s)	179, 773, 1304, 1659, 2185
Asp700I	GAANNNNTTC	2 cut(s)	1710, 1985
AspLEI	GCGC	1 cut(s)	7
AspS9I	GGNCC	1 cut(s)	279
AsuHPI	GGTGA	4 cut(s)	383, 1259, 1598, 1756
AsuNHI	GCTAGC	1 cut(s)	880
AvaI	CYCGRG	2 cut(s)	2075, 2154
AvaII	GGWCC	1 cut(s)	279
BanII	GRGCYC	1 cut(s)	2178
BbsI	GAAGAC	2 cut(s)	380, 817
Bbv12I	GWGCWC	4 cut(s)	374, 1825, 2155, 2178
BbvCI	CCTCAGC	1 cut(s)	2118
BbvI	GCAGC	5 cut(s)	191, 1450, 1466, 1700, 2101
BccI	CCATC	6 cut(s)	473, 692, 1046, 1136, 1154, 1811
BciT130I	CCWGG	4 cut(s)	172, 354, 1524, 2030
BclI	TGATCA	2 cut(s)	1360, 2199
BcoDI	GTCTC	2 cut(s)	132, 551
BcuI	ACTAGT	1 cut(s)	1483
BfaI	CTAG	3 cut(s)	881, 1326, 1484
BfmI	CTRYAG	1 cut(s)	613
BfoI	RGCGCY	1 cut(s)	8
BisI	GCNGC	5 cut(s)	205, 1455, 1464, 1689, 2115
BlsI	GCNGC	5 cut(s)	206, 1456, 1465, 1690, 2116
Bme1390I	CCNGG	4 cut(s)	172, 354, 1524, 2030
Bme18I	GGWCC	1 cut(s)	279
BmeT110I	CYCGRG	2 cut(s)	2075, 2154
BmgT120I	GGNCC	1 cut(s)	279
BmiI	GGNNCC	2 cut(s)	280, 289
BmrFI	CCNGG	4 cut(s)	172, 354, 1524, 2030
BmrI	ACTGGG	1 cut(s)	2044
BmsI	GCATC	7 cut(s)	501, 807, 1081, 1411, 1795, 2031, 2101
BmtI	GCTAGC	1 cut(s)	884
BmuI	ACTGGG	1 cut(s)	2044
BoxI	GACNNNNGTC	2 cut(s)	504, 1274
BpiI	GAAGAC	2 cut(s)	380, 817
BpmI	CTGGAG	2 cut(s)	274, 1061
Bpu10I	CCTNAGC	1 cut(s)	2118
BpuEI	CTTGAG	2 cut(s)	420, 1433
BsaBI	GATNNNNATC	1 cut(s)	930
BsaJI	CCNNGG	5 cut(s)	171, 264, 552, 1522, 2029
BsaWI	WCCGGW	2 cut(s)	1300, 2166
BsaXI	ACNNNNNCTCC	2 cut(s)	548, 578
Bsc4I	CCNNNNNNNGG	5 cut(s)	191, 1652, 2055, 2056, 2082
Bse1I	ACTGG	6 cut(s)	289, 291, 767, 1078, 1419, 2050
Bse3DI	GCAATG	1 cut(s)	1698
Bse8I	GATNNNNATC	1 cut(s)	930
BseAI	TCCGGA	1 cut(s)	1300
BseBI	CCWGG	4 cut(s)	172, 354, 1524, 2030
BseDI	CCNNGG	5 cut(s)	171, 264, 552, 1522, 2029
BseGI	GGATG	4 cut(s)	1402, 1687, 1827, 2116
BseJI	GATNNNNATC	1 cut(s)	930
BseLI	CCNNNNNNNGG	5 cut(s)	191, 1652, 2055, 2056, 2082
BseMI	GCAATG	1 cut(s)	1698
BseMII	CTCAG	3 cut(s)	660, 1640, 2109
BseNI	ACTGG	6 cut(s)	289, 291, 767, 1078, 1419, 2050
BseRI	GAGGAG	6 cut(s)	15, 18, 21, 24, 70, 143
BseXI	GCAGC	5 cut(s)	191, 1450, 1466, 1700, 2101
BseYI	CCCAGC	1 cut(s)	532
Bsh1236I	CGCG	1 cut(s)	2192
BshFI	GGCC	6 cut(s)	59, 352, 811, 1314, 1637, 2028
BsiHKAI	GWGCWC	4 cut(s)	374, 1825, 2155, 2178
BsiHKCI	CYCGRG	2 cut(s)	2075, 2154
BsiSI	CCGG	3 cut(s)	441, 1301, 2167
BslFI	GGGAC	4 cut(s)	129, 292, 390, 2125
BslI	CCNNNNNNNGG	5 cut(s)	191, 1652, 2055, 2056, 2082
BsmAI	GTCTC	2 cut(s)	132, 551
BsmBI	CGTCTC	1 cut(s)	132
BsmFI	GGGAC	4 cut(s)	129, 292, 390, 2125
BsmI	GAATGC	1 cut(s)	2002
BsnI	GGCC	6 cut(s)	59, 352, 811, 1314, 1637, 2028
BsoBI	CYCGRG	2 cut(s)	2075, 2154
Bsp1286I	GDGCHC	4 cut(s)	374, 1825, 2155, 2178
Bsp13I	TCCGGA	1 cut(s)	1300
Bsp143I	GATC	8 cut(s)	214, 307, 781, 906, 1360, 1508, 2199, 2209
BspACI	CCGC	8 cut(s)	88, 101, 176, 1016, 1348, 1910, 2046, 2190
BspANI	GGCC	6 cut(s)	59, 352, 811, 1314, 1637, 2028
BspCNI	CTCAG	3 cut(s)	661, 1641, 2110
BspEI	TCCGGA	1 cut(s)	1300
BspFNI	CGCG	1 cut(s)	2192
BspHI	TCATGA	2 cut(s)	903, 1021
BspLI	GGNNCC	2 cut(s)	280, 289
BspOI	GCTAGC	1 cut(s)	884
BspPI	GGATC	2 cut(s)	222, 315
BsrDI	GCAATG	1 cut(s)	1698
BsrI	ACTGG	6 cut(s)	289, 291, 767, 1078, 1419, 2050
BssECI	CCNNGG	5 cut(s)	171, 264, 552, 1522, 2029
BssMI	GATC	8 cut(s)	214, 307, 781, 906, 1360, 1508, 2199, 2209
BssT1I	CCWWGG	1 cut(s)	552
Bst2UI	CCWGG	4 cut(s)	172, 354, 1524, 2030
Bst4CI	ACNGT	4 cut(s)	562, 571, 688, 1138
Bst6I	CTCTTC	4 cut(s)	115, 127, 161, 1701
BstC8I	GCNNGC	5 cut(s)	138, 474, 882, 1938, 2046
BstDEI	CTNAG	5 cut(s)	331, 669, 914, 1649, 2118
BstF5I	GGATG	4 cut(s)	1402, 1687, 1827, 2116
BstFNI	CGCG	1 cut(s)	2192
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	1 cut(s)	7
BstKTI	GATC	8 cut(s)	217, 310, 784, 909, 1363, 1511, 2202, 2212
BstMAI	GTCTC	2 cut(s)	132, 551
BstMBI	GATC	8 cut(s)	214, 307, 781, 906, 1360, 1508, 2199, 2209
BstMWI	GCNNNNNNNGC	8 cut(s)	201, 266, 446, 1451, 1460, 1463, 1472, 2120
BstNI	CCWGG	4 cut(s)	172, 354, 1524, 2030
BstNSI	RCATGY	2 cut(s)	581, 1256
BstPAI	GACNNNNGTC	2 cut(s)	504, 1274
BstSCI	CCNGG	4 cut(s)	170, 352, 1522, 2028
BstSFI	CTRYAG	1 cut(s)	613
BstUI	CGCG	1 cut(s)	2192
BstV1I	GCAGC	5 cut(s)	191, 1450, 1466, 1700, 2101
BstV2I	GAAGAC	2 cut(s)	380, 817
BsuRI	GGCC	6 cut(s)	59, 352, 811, 1314, 1637, 2028
BtsCI	GGATG	4 cut(s)	1402, 1687, 1827, 2116
BtsI	GCAGTG	1 cut(s)	255
BtsIMutI	CAGTG	6 cut(s)	255, 282, 298, 495, 1085, 1646
Cac8I	GCNNGC	5 cut(s)	138, 474, 882, 1938, 2046
CaiI	CAGNNNCTG	1 cut(s)	1808
CciI	TCATGA	2 cut(s)	903, 1021
CfoI	GCGC	1 cut(s)	7
Cfr13I	GGNCC	1 cut(s)	279
CseI	GACGC	1 cut(s)	1989
Csp6I	GTAC	3 cut(s)	761, 1412, 1833
CspCI	CAANNNNNGTGG	2 cut(s)	490, 525
CviQI	GTAC	3 cut(s)	761, 1412, 1833
DdeI	CTNAG	5 cut(s)	331, 669, 914, 1649, 2118
DpnI	GATC	8 cut(s)	216, 309, 783, 908, 1362, 1510, 2201, 2211
DpnII	GATC	8 cut(s)	214, 307, 781, 906, 1360, 1508, 2199, 2209
DraI	TTTAAA	1 cut(s)	1440
DrdI	GACNNNNNNGTC	2 cut(s)	384, 821
DseDI	GACNNNNNNGTC	2 cut(s)	384, 821
Eam1104I	CTCTTC	4 cut(s)	115, 127, 161, 1701
EarI	CTCTTC	4 cut(s)	115, 127, 161, 1701
EciI	GGCGGA	2 cut(s)	77, 191
Ecl136II	GAGCTC	1 cut(s)	2176
Eco130I	CCWWGG	1 cut(s)	552
Eco147I	AGGCCT	1 cut(s)	2028
Eco24I	GRGCYC	1 cut(s)	2178
Eco32I	GATATC	2 cut(s)	928, 1862
Eco47I	GGWCC	1 cut(s)	279
Eco53kI	GAGCTC	1 cut(s)	2176
Eco57I	CTGAAG	3 cut(s)	1578, 1840, 1961
Eco88I	CYCGRG	2 cut(s)	2075, 2154
EcoICRI	GAGCTC	1 cut(s)	2176
EcoRI	GAATTC	2 cut(s)	773, 2185
EcoRII	CCWGG	4 cut(s)	170, 352, 1522, 2028
EcoRV	GATATC	2 cut(s)	928, 1862
EcoT14I	CCWWGG	1 cut(s)	552
EcoT22I	ATGCAT	1 cut(s)	1096
EcoT38I	GRGCYC	1 cut(s)	2178
ErhI	CCWWGG	1 cut(s)	552
Esp3I	CGTCTC	1 cut(s)	132
FalI	AAGNNNNNCTT	4 cut(s)	1365, 1397, 1950, 1982
FaqI	GGGAC	4 cut(s)	129, 292, 390, 2125
FauI	CCCGC	2 cut(s)	108, 2053
FbaI	TGATCA	2 cut(s)	1360, 2199
FblI	GTMKAC	1 cut(s)	251
Fnu4HI	GCNGC	5 cut(s)	205, 1455, 1464, 1689, 2115
FokI	GGATG	4 cut(s)	1389, 1694, 1814, 2123
FriOI	GRGCYC	1 cut(s)	2178
Fsp4HI	GCNGC	5 cut(s)	205, 1455, 1464, 1689, 2115
FspBI	CTAG	3 cut(s)	881, 1326, 1484
GlaI	GCGC	1 cut(s)	6
GluI	GCNGC	5 cut(s)	205, 1455, 1464, 1689, 2115
GsaI	CCCAGC	1 cut(s)	536
GsuI	CTGGAG	2 cut(s)	274, 1061
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	6 cut(s)	59, 352, 811, 1314, 1637, 2028
HapII	CCGG	3 cut(s)	441, 1301, 2167
HgaI	GACGC	1 cut(s)	1989
HhaI	GCGC	1 cut(s)	7
Hin6I	GCGC	1 cut(s)	5
HinP1I	GCGC	1 cut(s)	5
HincII	GTYRAC	2 cut(s)	427, 567
HindII	GTYRAC	2 cut(s)	427, 567
HinfI	GANTC	8 cut(s)	313, 514, 1056, 1418, 1562, 1589, 1711, 2071
HpaII	CCGG	3 cut(s)	441, 1301, 2167
HphI	GGTGA	4 cut(s)	383, 1259, 1598, 1756
Hpy166II	GTNNAC	3 cut(s)	252, 427, 567
Hpy8I	GTNNAC	3 cut(s)	252, 427, 567
HpyCH4III	ACNGT	4 cut(s)	562, 571, 688, 1138
HpyCH4IV	ACGT	2 cut(s)	1275, 1777
HpyCH4V	TGCA	9 cut(s)	233, 260, 422, 529, 634, 1094, 1402, 1466, 1607
HpyF10VI	GCNNNNNNNGC	8 cut(s)	201, 266, 446, 1451, 1460, 1463, 1472, 2120
HpyF3I	CTNAG	5 cut(s)	331, 669, 914, 1649, 2118
HpySE526I	ACGT	2 cut(s)	1275, 1777
HspAI	GCGC	1 cut(s)	5
Kpn2I	TCCGGA	1 cut(s)	1300
Ksp22I	TGATCA	2 cut(s)	1360, 2199
Kzo9I	GATC	8 cut(s)	214, 307, 781, 906, 1360, 1508, 2199, 2209
LmnI	GCTCC	4 cut(s)	293, 369, 877, 2173
Lsp1109I	GCAGC	5 cut(s)	191, 1450, 1466, 1700, 2101
LweI	GCATC	7 cut(s)	501, 807, 1081, 1411, 1795, 2031, 2101
MaeI	CTAG	3 cut(s)	881, 1326, 1484
MaeII	ACGT	2 cut(s)	1275, 1777
MaeIII	GTNAC	3 cut(s)	682, 739, 1778
MalI	GATC	8 cut(s)	216, 309, 783, 908, 1362, 1510, 2201, 2211
MboI	GATC	8 cut(s)	214, 307, 781, 906, 1360, 1508, 2199, 2209
MhlI	GDGCHC	4 cut(s)	374, 1825, 2155, 2178
MluCI	AATT	7 cut(s)	179, 773, 870, 1304, 1659, 1722, 2185
MmeI	TCCRAC	3 cut(s)	285, 542, 2196
Mph1103I	ATGCAT	1 cut(s)	1096
MroI	TCCGGA	1 cut(s)	1300
MroXI	GAANNNNTTC	2 cut(s)	1710, 1985
MseI	TTAA	6 cut(s)	1178, 1439, 1799, 1968, 2091, 2224
MslI	CAYNNNNRTG	4 cut(s)	582, 951, 1158, 1407
MspA1I	CMGCKG	1 cut(s)	1595
MspI	CCGG	3 cut(s)	441, 1301, 2167
MspR9I	CCNGG	4 cut(s)	172, 354, 1524, 2030
Mva1269I	GAATGC	1 cut(s)	2002
MvaI	CCWGG	4 cut(s)	172, 354, 1524, 2030
MvnI	CGCG	1 cut(s)	2192
MwoI	GCNNNNNNNGC	8 cut(s)	201, 266, 446, 1451, 1460, 1463, 1472, 2120
NdeII	GATC	8 cut(s)	214, 307, 781, 906, 1360, 1508, 2199, 2209
NheI	GCTAGC	1 cut(s)	880
NlaIV	GGNNCC	2 cut(s)	280, 289
NmuCI	GTSAC	1 cut(s)	739
NsiI	ATGCAT	1 cut(s)	1096
NspI	RCATGY	2 cut(s)	581, 1256
PagI	TCATGA	2 cut(s)	903, 1021
PceI	AGGCCT	1 cut(s)	2028
PctI	GAATGC	1 cut(s)	2002
PdmI	GAANNNNTTC	2 cut(s)	1710, 1985
PfeI	GAWTC	8 cut(s)	313, 514, 1056, 1418, 1562, 1589, 1711, 2071
PkrI	GCNGC	5 cut(s)	206, 1456, 1465, 1690, 2116
PshAI	GACNNNNGTC	2 cut(s)	504, 1274
Psp124BI	GAGCTC	1 cut(s)	2178
Psp6I	CCWGG	4 cut(s)	170, 352, 1522, 2028
PspFI	CCCAGC	1 cut(s)	532
PspGI	CCWGG	4 cut(s)	170, 352, 1522, 2028
PspN4I	GGNNCC	2 cut(s)	280, 289
PspPI	GGNCC	1 cut(s)	279
PstNI	CAGNNNCTG	1 cut(s)	1808
PvuII	CAGCTG	1 cut(s)	1595
RsaI	GTAC	3 cut(s)	762, 1413, 1834
RsaNI	GTAC	3 cut(s)	761, 1412, 1833
RseI	CAYNNNNRTG	4 cut(s)	582, 951, 1158, 1407
SacI	GAGCTC	1 cut(s)	2178
SaqAI	TTAA	6 cut(s)	1178, 1439, 1799, 1968, 2091, 2224
SatI	GCNGC	5 cut(s)	205, 1455, 1464, 1689, 2115
Sau3AI	GATC	8 cut(s)	214, 307, 781, 906, 1360, 1508, 2199, 2209
Sau96I	GGNCC	1 cut(s)	279
ScrFI	CCNGG	4 cut(s)	172, 354, 1524, 2030
SduI	GDGCHC	4 cut(s)	374, 1825, 2155, 2178
SfaNI	GCATC	7 cut(s)	501, 807, 1081, 1411, 1795, 2031, 2101
SfcI	CTRYAG	1 cut(s)	613
SinI	GGWCC	1 cut(s)	279
SmiMI	CAYNNNNRTG	4 cut(s)	582, 951, 1158, 1407
SmlI	CTYRAG	2 cut(s)	435, 1448
SmoI	CTYRAG	2 cut(s)	435, 1448
SpeI	ACTAGT	1 cut(s)	1483
Sse9I	AATT	7 cut(s)	179, 773, 870, 1304, 1659, 1722, 2185
SseBI	AGGCCT	1 cut(s)	2028
SsiI	CCGC	8 cut(s)	88, 101, 176, 1016, 1348, 1910, 2046, 2190
SspI	AATATT	2 cut(s)	411, 1946
SspMI	CTAG	3 cut(s)	881, 1326, 1484
SstI	GAGCTC	1 cut(s)	2178
StuI	AGGCCT	1 cut(s)	2028
StyD4I	CCNGG	4 cut(s)	170, 352, 1522, 2028
StyI	CCWWGG	1 cut(s)	552
TaaI	ACNGT	4 cut(s)	562, 571, 688, 1138
TaiI	ACGT	2 cut(s)	1278, 1780
TaqI	TCGA	3 cut(s)	19, 217, 2208
TaqII	GACCGA	1 cut(s)	1273
TasI	AATT	7 cut(s)	179, 773, 870, 1304, 1659, 1722, 2185
TatI	WGTACW	2 cut(s)	760, 1411
TfiI	GAWTC	8 cut(s)	313, 514, 1056, 1418, 1562, 1589, 1711, 2071
Tru1I	TTAA	6 cut(s)	1178, 1439, 1799, 1968, 2091, 2224
Tru9I	TTAA	6 cut(s)	1178, 1439, 1799, 1968, 2091, 2224
TscAI	CASTG	6 cut(s)	262, 289, 298, 502, 1085, 1653
TseFI	GTSAC	1 cut(s)	739
TseI	GCWGC	5 cut(s)	204, 1454, 1463, 1688, 2114
Tsp45I	GTSAC	1 cut(s)	739
TspGWI	ACGGA	3 cut(s)	1147, 1400, 1820
TspRI	CASTG	6 cut(s)	262, 289, 298, 502, 1085, 1653
VpaK11BI	GGWCC	1 cut(s)	279
XapI	RAATTY	5 cut(s)	179, 773, 1304, 1659, 2185
XceI	RCATGY	2 cut(s)	581, 1256
XcmI	CCANNNNNNNNNTGG	1 cut(s)	785
XmiI	GTMKAC	1 cut(s)	251
XmnI	GAANNNNTTC	2 cut(s)	1710, 1985
XspI	CTAG	3 cut(s)	881, 1326, 1484
Zsp2I	ATGCAT	1 cut(s)	1096

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.