Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0006623

Physical Location & Seq

Genomic Coordinates Forward (+)

39916862 .. 39930563

Gene Structure 13702 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG326100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2214 bp

ATGGCGCTTGTTCTTCTGAGACTGAGAGCTCATTCTCCTAGTCCTCCTTATCTTCTTCGCTTCATGGCCTTACGCTTCATGTCACACTCATGTCTCCACCCTCACTCTTCCTCCCTTGCTCGTCCCTCCCTCTTGTTCAGCCCACCAGCACCAGCACCACCCAGGATTGGGCTTCGATTGAAGGACGTTCCTAGCTGCTGTGCTGCGGTTCGAAATGTGAGAGCTTTCATGTCGTCCAGCTCGGTAACCGAGGCTTTTCAAGGAAGCGCTGCGGCCTCCACGGCTTATGGCGCTGATCAGATTCAGGTTTTAGAAGGCTTAGAGGCTGTTAGAAAGAGGCCCGGAATGTATATTGGGGACACCGGATTTCGTGGTCTTCATCATCTGGTTTATGAAATACTAGACAATGCTGTTGATGAGGCTCAAGGTGGATATGCTTCAAAGGTTGAGGTCATTTTACTGGCCGACAATTCTGTCAGCATCACAGACAACGGCCGTGGGATACCTACTGATATGCATCCTGTAACCAAGAAATCTACATTGGAGACTGTCTTGACGGTTTTGCATGCTGGTGGCAAGTTTGGTGGTTCAAGTAGTGGCTATAGCGTGTCTGGTGGATTACATGGTGTGGGTTTATCAGTCGTTAATGCCTTGTCTGAGGCATTAGAAGTTACTATTTGGCGTGATGGAATGGAATACCAGCAAAAGTATTCTCGTGGAAAGCCTGTGACAACTCTCTCTTGTCATGTGCTTCCAATTGAATTTAAAGATAGACAAGGAACACGAATCAGATTCTTGCCTGACAAACAAGTATTTAAAACTCTTAATCAGTTTGACTACCACACAATTGCTGGAAGAATTAGGGAGCTAGCTTTTTTAAACCCTAATCTCATGATCACTCTTAGACAAGAGGGTACGGATCCAGAGAAAATCCAGCACAATGAATACTTCTATGCAGGGGGTTTGGTTGAATATGTTAGATGGCTAAATACTGATAAGAAACCTCTTCATGAAGTGGTGGGTTTCAGAAAAGAAGTAGATGGAATCACTATTAATGTAGCTCTCCAGTGGTGTTCTGATGCATATTCAGATACAGTGCTAGGGTATGCTAATAGCATACGTACTGCTGACGGAGGAACCCATATTGATGGACTGAAAGCTTCGTTAACAAGAACGCTGAATAACCTTGGGAAGAAGTCAAAGCTTATCAAGGATAAAGATATTACCTTAAGTGGTGAGCATGTAAGAGAAGGGCTGACATGTGTAGTTTCAGTCAAAGTTCCCAATCCAGAGTTTGAAGGCCAGACAAAGACTAGGTTGGGAAATCCGGAGGTGCGAAGAGTGGTTGATAATTCTCTTCAAGAGTATCTTACAGAATACTTGGAGTTACATCCTGATGTACTGGATTCAATCCTTTCCAAGTCCTTAAATGCTCTCAAGGCAGCTCTGGCTGCAAAGAGGGCAAGGGAACTAGTCAGACAAAAGAGTGTTTTGCGTTCGTCTTCTCTCCCAGGAAAGCTAGCTGATTGCTCATCAACCAATCCTGAAGAATCTGAAATCTTTATAGTTGAAGGAGATTCAGCTGGAGGAAGTGCAAAGCAAGGTCGTGATAGACGCTTTCAGGCAATTCTTCCACTAAGGGGTAAAATTCTGAACATTGAAAGGAGGGACGAGGCAGCGATGTATAAAAATGAAGAAATTCAAAATCTGATTCTTGGACTTGGACTTGGGGTGAAGGGAGAGGACTTTAAAAAGGAAGCTCTGCGTTACCATAAGATTATCATCTTAACTGATGCTGATGTAGATGGTGCTCACATCCGAACTCTGCTACTGACTTTTTTCTTCAGATATCAGAGAGCCTTATTTGATGAGGGTTGCATATATGTTGGTGTTCCACCCTTTTACAAGGTTGAAAGGGGAAAGCAAGCATATTATTGCTATGATGACGCTGAACTTAAAAAGCTTCAGAGTTCCTTCCCTTCAAATGCATCATACAGCATTCAAAGGTTCAAAGGCCTGGGAGAAATGATGCCCACACAATTATGGGAAACAACAATGGATCCTCAAAAAAGGCTACTGAAACAATTAAGTGTGGATGATGCTGCTGAGGCTAATTTTGTCTTTTCCTCCCTTATGGGTTCTCGGGTGGATTTCCGGAAGGAACTCATTCAAAATGCTGCACGCATGATCAGCTTGGATCAGTTAGATATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003916 GO:0003918 GO:0005575 GO:0006139 GO:0006259 GO:0006265 GO:0006725 GO:0006807 GO:0006996 GO:0007059 GO:0008094 GO:0008150 GO:0008152 GO:0009295 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0017111 GO:0034641 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0051276 GO:0061505 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_gyraseB DNA_gyraseB_C HATPase_c Toprim

Protein Analysis

737

Amino Acids

81.82

Weight (kDa)

8.68

Isoelectric Point (pI)

42.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HATPase_c	PF02518	125 - 267	4.5e-17	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
DNA_gyraseB	PF00204	321 - 490	3.6e-55	DNA gyrase B
Toprim	PF01751	519 - 619	5.5e-18	Toprim domain
DNA_gyraseB_C	PF00986	663 - 724	5.8e-25	DNA gyrase B subunit, carboxyl terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006623)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10270 AT3G10270 AT5G04130
fragaria_vesca	FvH4_6g25390 FvH4_6g25390 FvH4_6g25390
malus_domestica	MD12G1095800.v1.1 MD14G1090600.v1.1
prunus_persica	Prupe.7G033800_v2.0.a1
pyrus_communis	pycom12g08270
rosa_chinensis	RchiOBHm_Chr2g0125831
rosa_laevigata	RLG00000018881
rosa_multiflora	Rmu_co8103446.1_g000001 Rmu_sc0009079.1_g000002
rosa_roxburghii	Rroxscaffold_2G00117620 Rroxscaffold_2G00117680 Rroxscaffold_2G00125340 Rroxscaffold_2G00125370 Rroxscaffold_3G00238600 Rroxscaffold_4G00318520 Rroxscaffold_4G00324150 Rroxscaffold_5G00337460
rosa_rugosa	Rorug02G0259400
rosa_samantha	Rh2AG317700 Rh2BG326100
rosa_wichuraiana	Rw2G025700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	473
AccIII	TCCGGA	2 cut(s)	1327, 2154
AciI	CCGC	2 cut(s)	206, 272
AclWI	GGATC	5 cut(s)	914, 927, 2054, 2067, 2205
AcoI	YGGCCR	2 cut(s)	462, 493
AcsI	RAATTY	3 cut(s)	761, 1647, 1698
AcuI	CTGAAG	3 cut(s)	1566, 1828, 1949
AfaI	GTAC	3 cut(s)	916, 1123, 1401
AfeI	AGCGCT	1 cut(s)	268
AfiI	CCNNNNNNNGG	3 cut(s)	167, 168, 1640
AflII	CTTAAG	1 cut(s)	1228
AflIII	ACRYGT	1 cut(s)	1259
AhlI	ACTAGT	1 cut(s)	1471
AjnI	CCWGG	3 cut(s)	161, 1510, 2016
AjuI	GAANNNNNNNTTGG	2 cut(s)	524, 556
Alw21I	GWGCWC	2 cut(s)	31, 1813
Alw26I	GTCTC	3 cut(s)	13, 98, 539
AlwI	GGATC	5 cut(s)	914, 927, 2054, 2067, 2205
Ama87I	CYCGRG	1 cut(s)	2142
Aor13HI	TCCGGA	2 cut(s)	1327, 2154
Aor51HI	AGCGCT	1 cut(s)	268
AoxI	GGCC	7 cut(s)	66, 273, 338, 462, 493, 1300, 2014
ApeKI	GCWGC	8 cut(s)	195, 203, 269, 1442, 1451, 1676, 2102, 2177
ApoI	RAATTY	3 cut(s)	761, 1647, 1698
AseI	ATTAAT	1 cut(s)	1053
Asp700I	GAANNNNTTC	2 cut(s)	1698, 2166
AspLEI	GCGC	3 cut(s)	7, 269, 293
AspS9I	GGNCC	1 cut(s)	339
AsuC2I	CCSGG	1 cut(s)	342
AsuHPI	GGTGA	2 cut(s)	1247, 1744
AsuII	TTCGAA	1 cut(s)	211
AsuNHI	GCTAGC	2 cut(s)	868, 1519
AvaI	CYCGRG	1 cut(s)	2142
BamHI	GGATCC	2 cut(s)	919, 2059
BanII	GRGCYC	1 cut(s)	31
BauI	CACGAG	1 cut(s)	714
BbsI	GAAGAC	2 cut(s)	368, 1494
Bbv12I	GWGCWC	2 cut(s)	31, 1813
BbvCI	CCTCAGC	1 cut(s)	2106
BbvI	GCAGC	8 cut(s)	182, 190, 256, 1438, 1454, 1688, 2089, 2164
BccI	CCATC	5 cut(s)	680, 975, 1034, 1142, 1799
BceAI	ACGGC	3 cut(s)	297, 480, 508
BciT130I	CCWGG	3 cut(s)	163, 1512, 2018
BciVI	GTATCC	1 cut(s)	495
BclI	TGATCA	3 cut(s)	295, 894, 2187
BcnI	CCSGG	1 cut(s)	342
BcoDI	GTCTC	3 cut(s)	13, 98, 539
BcuI	ACTAGT	1 cut(s)	1471
BfaI	CTAG	8 cut(s)	39, 192, 401, 869, 1100, 1314, 1472, 1520
BfmI	CTRYAG	1 cut(s)	601
BfoI	RGCGCY	3 cut(s)	8, 270, 294
BfrI	CTTAAG	1 cut(s)	1228
BfuI	GTATCC	1 cut(s)	495
BglI	GCCNNNNNGGC	1 cut(s)	281
BisI	GCNGC	9 cut(s)	196, 204, 270, 273, 1443, 1452, 1677, 2103, 2178
BlsI	GCNGC	9 cut(s)	197, 205, 271, 274, 1444, 1453, 1678, 2104, 2179
Bme1390I	CCNGG	4 cut(s)	163, 342, 1512, 2018
BmeT110I	CYCGRG	1 cut(s)	2142
BmgT120I	GGNCC	1 cut(s)	339
BmiI	GGNNCC	3 cut(s)	921, 1138, 2061
BmrFI	CCNGG	4 cut(s)	163, 342, 1512, 2018
BmsI	GCATC	7 cut(s)	489, 526, 1069, 1783, 1997, 2019, 2089
BmtI	GCTAGC	2 cut(s)	872, 1523
BpiI	GAAGAC	2 cut(s)	368, 1494
BpmI	CTGGAG	2 cut(s)	1049, 1605
Bpu10I	CCTNAGC	1 cut(s)	2106
Bpu14I	TTCGAA	1 cut(s)	211
BpuEI	CTTGAG	2 cut(s)	408, 1421
BpuMI	CCSGG	1 cut(s)	342
BsaAI	YACGTR	1 cut(s)	1121
BsaBI	GATNNNNATC	2 cut(s)	516, 1781
BsaJI	CCNNGG	7 cut(s)	161, 249, 279, 496, 1186, 1510, 2017
BsaWI	WCCGGW	3 cut(s)	362, 1327, 2154
BsaXI	ACNNNNNCTCC	2 cut(s)	95, 125
Bsc4I	CCNNNNNNNGG	3 cut(s)	167, 168, 1640
Bse1I	ACTGG	3 cut(s)	465, 1066, 1407
Bse8I	GATNNNNATC	2 cut(s)	516, 1781
BseAI	TCCGGA	2 cut(s)	1327, 2154
BseBI	CCWGG	3 cut(s)	163, 1512, 2018
BseDI	CCNNGG	7 cut(s)	161, 249, 279, 496, 1186, 1510, 2017
BseGI	GGATG	4 cut(s)	517, 1390, 1815, 2101
BseJI	GATNNNNATC	2 cut(s)	516, 1781
BseLI	CCNNNNNNNGG	3 cut(s)	167, 168, 1640
BseMII	CTCAG	4 cut(s)	8, 14, 648, 2097
BseNI	ACTGG	3 cut(s)	465, 1066, 1407
BseX3I	CGGCCG	1 cut(s)	493
BseXI	GCAGC	8 cut(s)	182, 190, 256, 1438, 1454, 1688, 2089, 2164
BsgI	GTGCAG	1 cut(s)	2163
Bsh1285I	CGRYCG	1 cut(s)	496
BshFI	GGCC	7 cut(s)	68, 275, 340, 464, 495, 1302, 2016
BsiEI	CGRYCG	1 cut(s)	496
BsiHKAI	GWGCWC	2 cut(s)	31, 1813
BsiHKCI	CYCGRG	1 cut(s)	2142
BsiSI	CCGG	4 cut(s)	342, 363, 1328, 2155
BslFI	GGGAC	3 cut(s)	108, 371, 1682
BslI	CCNNNNNNNGG	3 cut(s)	167, 168, 1640
BsmAI	GTCTC	3 cut(s)	13, 98, 539
BsmFI	GGGAC	3 cut(s)	108, 371, 1682
BsmI	GAATGC	1 cut(s)	1998
BsnI	GGCC	7 cut(s)	68, 275, 340, 464, 495, 1302, 2016
BsoBI	CYCGRG	1 cut(s)	2142
Bsp119I	TTCGAA	1 cut(s)	211
Bsp1286I	GDGCHC	2 cut(s)	31, 1813
Bsp13I	TCCGGA	2 cut(s)	1327, 2154
Bsp143I	GATC	6 cut(s)	295, 894, 919, 2059, 2187, 2197
BspACI	CCGC	2 cut(s)	206, 272
BspANI	GGCC	7 cut(s)	68, 275, 340, 464, 495, 1302, 2016
BspCNI	CTCAG	4 cut(s)	9, 15, 649, 2098
BspEI	TCCGGA	2 cut(s)	1327, 2154
BspHI	TCATGA	2 cut(s)	891, 1009
BspLI	GGNNCC	3 cut(s)	921, 1138, 2061
BspOI	GCTAGC	2 cut(s)	872, 1523
BspPI	GGATC	5 cut(s)	914, 927, 2054, 2067, 2205
BspT104I	TTCGAA	1 cut(s)	211
BspTI	CTTAAG	1 cut(s)	1228
BsrI	ACTGG	3 cut(s)	465, 1066, 1407
BssECI	CCNNGG	7 cut(s)	161, 249, 279, 496, 1186, 1510, 2017
BssMI	GATC	6 cut(s)	295, 894, 919, 2059, 2187, 2197
BssSI	CACGAG	1 cut(s)	714
BssT1I	CCWWGG	1 cut(s)	1186
Bst2BI	CACGAG	1 cut(s)	714
Bst2UI	CCWGG	3 cut(s)	163, 1512, 2018
Bst4CI	ACNGT	3 cut(s)	550, 559, 1096
Bst6I	CTCTTC	4 cut(s)	112, 1011, 1333, 1362
BstAFI	CTTAAG	1 cut(s)	1228
BstBAI	YACGTR	1 cut(s)	1121
BstBI	TTCGAA	1 cut(s)	211
BstC8I	GCNNGC	5 cut(s)	567, 870, 1521, 1926, 2182
BstDEI	CTNAG	7 cut(s)	17, 23, 319, 657, 902, 1637, 2106
BstDSI	CCRYGG	2 cut(s)	279, 496
BstEII	GGTNACC	1 cut(s)	244
BstF5I	GGATG	4 cut(s)	517, 1390, 1815, 2101
BstH2I	RGCGCY	3 cut(s)	8, 270, 294
BstHHI	GCGC	3 cut(s)	7, 269, 293
BstKTI	GATC	6 cut(s)	298, 897, 922, 2062, 2190, 2200
BstMAI	GTCTC	3 cut(s)	13, 98, 539
BstMBI	GATC	6 cut(s)	295, 894, 919, 2059, 2187, 2197
BstMCI	CGRYCG	1 cut(s)	496
BstMWI	GCNNNNNNNGC	8 cut(s)	281, 290, 1439, 1448, 1451, 1460, 2108, 2190
BstNI	CCWGG	3 cut(s)	163, 1512, 2018
BstNSI	RCATGY	3 cut(s)	569, 1244, 1263
BstPI	GGTNACC	1 cut(s)	244
BstSCI	CCNGG	4 cut(s)	161, 340, 1510, 2016
BstSFI	CTRYAG	1 cut(s)	601
BstSNI	TACGTA	1 cut(s)	1121
BstV1I	GCAGC	8 cut(s)	182, 190, 256, 1438, 1454, 1688, 2089, 2164
BstV2I	GAAGAC	2 cut(s)	368, 1494
BstX2I	RGATCY	2 cut(s)	919, 2059
BstXI	CCANNNNNNTGG	1 cut(s)	1148
BstYI	RGATCY	2 cut(s)	919, 2059
BstZI	CGGCCG	1 cut(s)	493
BsuI	GTATCC	1 cut(s)	495
BsuRI	GGCC	7 cut(s)	68, 275, 340, 464, 495, 1302, 2016
BtgI	CCRYGG	2 cut(s)	279, 496
BtgZI	GCGATG	1 cut(s)	1694
BtsCI	GGATG	4 cut(s)	517, 1390, 1815, 2101
BtsIMutI	CAGTG	2 cut(s)	1073, 1101
Cac8I	GCNNGC	5 cut(s)	567, 870, 1521, 1926, 2182
CciI	TCATGA	2 cut(s)	891, 1009
CfoI	GCGC	3 cut(s)	7, 269, 293
Cfr13I	GGNCC	1 cut(s)	339
CseI	GACGC	2 cut(s)	1623, 1955
Csp6I	GTAC	3 cut(s)	915, 1122, 1400
CspCI	CAANNNNNGTGG	4 cut(s)	478, 513, 565, 600
CviQI	GTAC	3 cut(s)	915, 1122, 1400
DdeI	CTNAG	7 cut(s)	17, 23, 319, 657, 902, 1637, 2106
DpnI	GATC	6 cut(s)	297, 896, 921, 2061, 2189, 2199
DpnII	GATC	6 cut(s)	295, 894, 919, 2059, 2187, 2197
DraI	TTTAAA	4 cut(s)	766, 817, 879, 1750
DrdI	GACNNNNNNGTC	1 cut(s)	473
DseDI	GACNNNNNNGTC	1 cut(s)	473
EaeI	YGGCCR	2 cut(s)	462, 493
EagI	CGGCCG	1 cut(s)	493
Eam1104I	CTCTTC	4 cut(s)	112, 1011, 1333, 1362
EarI	CTCTTC	4 cut(s)	112, 1011, 1333, 1362
Ecl136II	GAGCTC	1 cut(s)	29
EclXI	CGGCCG	1 cut(s)	493
Eco105I	TACGTA	1 cut(s)	1121
Eco130I	CCWWGG	1 cut(s)	1186
Eco147I	AGGCCT	1 cut(s)	2016
Eco24I	GRGCYC	1 cut(s)	31
Eco32I	GATATC	1 cut(s)	1850
Eco47III	AGCGCT	1 cut(s)	268
Eco52I	CGGCCG	1 cut(s)	493
Eco53kI	GAGCTC	1 cut(s)	29
Eco57I	CTGAAG	3 cut(s)	1566, 1828, 1949
Eco88I	CYCGRG	1 cut(s)	2142
Eco91I	GGTNACC	1 cut(s)	244
EcoICRI	GAGCTC	1 cut(s)	29
EcoO65I	GGTNACC	1 cut(s)	244
EcoRII	CCWGG	3 cut(s)	161, 1510, 2016
EcoRV	GATATC	1 cut(s)	1850
EcoT14I	CCWWGG	1 cut(s)	1186
EcoT22I	ATGCAT	3 cut(s)	519, 1084, 1990
EcoT38I	GRGCYC	1 cut(s)	31
ErhI	CCWWGG	1 cut(s)	1186
FalI	AAGNNNNNCTT	2 cut(s)	1353, 1385
FaqI	GGGAC	3 cut(s)	108, 371, 1682
FbaI	TGATCA	3 cut(s)	295, 894, 2187
Fnu4HI	GCNGC	9 cut(s)	196, 204, 270, 273, 1443, 1452, 1677, 2103, 2178
FokI	GGATG	4 cut(s)	504, 1377, 1802, 2108
FriOI	GRGCYC	1 cut(s)	31
Fsp4HI	GCNGC	9 cut(s)	196, 204, 270, 273, 1443, 1452, 1677, 2103, 2178
FspBI	CTAG	8 cut(s)	39, 192, 401, 869, 1100, 1314, 1472, 1520
GlaI	GCGC	3 cut(s)	6, 268, 292
GluI	GCNGC	9 cut(s)	196, 204, 270, 273, 1443, 1452, 1677, 2103, 2178
GsuI	CTGGAG	2 cut(s)	1049, 1605
HaeII	RGCGCY	3 cut(s)	8, 270, 294
HaeIII	GGCC	7 cut(s)	68, 275, 340, 464, 495, 1302, 2016
HapII	CCGG	4 cut(s)	342, 363, 1328, 2155
HgaI	GACGC	2 cut(s)	1623, 1955
HhaI	GCGC	3 cut(s)	7, 269, 293
Hin6I	GCGC	3 cut(s)	5, 267, 291
HinP1I	GCGC	3 cut(s)	5, 267, 291
HincII	GTYRAC	1 cut(s)	1167
HindII	GTYRAC	1 cut(s)	1167
HindIII	AAGCTT	3 cut(s)	1158, 1202, 1961
HinfI	GANTC	8 cut(s)	301, 786, 792, 1044, 1406, 1550, 1577, 1711
HpaI	GTTAAC	1 cut(s)	1167
HpaII	CCGG	4 cut(s)	342, 363, 1328, 2155
HphI	GGTGA	2 cut(s)	1247, 1744
Hpy166II	GTNNAC	1 cut(s)	1167
Hpy8I	GTNNAC	1 cut(s)	1167
HpyAV	CCTTC	9 cut(s)	175, 308, 1244, 1292, 1565, 1729, 1984, 1989, 2152
HpyCH4III	ACNGT	3 cut(s)	550, 559, 1096
HpyCH4IV	ACGT	2 cut(s)	186, 1120
HpyCH4V	TGCA	9 cut(s)	517, 565, 956, 1082, 1454, 1595, 1878, 1988, 2180
HpyF10VI	GCNNNNNNNGC	8 cut(s)	281, 290, 1439, 1448, 1451, 1460, 2108, 2190
HpyF3I	CTNAG	7 cut(s)	17, 23, 319, 657, 902, 1637, 2106
HpySE526I	ACGT	2 cut(s)	186, 1120
HspAI	GCGC	3 cut(s)	5, 267, 291
Kpn2I	TCCGGA	2 cut(s)	1327, 2154
Ksp22I	TGATCA	3 cut(s)	295, 894, 2187
KspAI	GTTAAC	1 cut(s)	1167
Kzo9I	GATC	6 cut(s)	295, 894, 919, 2059, 2187, 2197
LmnI	GCTCC	1 cut(s)	865
Lsp1109I	GCAGC	8 cut(s)	182, 190, 256, 1438, 1454, 1688, 2089, 2164
LweI	GCATC	7 cut(s)	489, 526, 1069, 1783, 1997, 2019, 2089
MaeI	CTAG	8 cut(s)	39, 192, 401, 869, 1100, 1314, 1472, 1520
MaeII	ACGT	2 cut(s)	186, 1120
MaeIII	GTNAC	7 cut(s)	81, 244, 523, 670, 727, 1386, 1766
MalI	GATC	6 cut(s)	297, 896, 921, 2061, 2189, 2199
MboI	GATC	6 cut(s)	295, 894, 919, 2059, 2187, 2197
MfeI	CAATTG	2 cut(s)	756, 846
MflI	RGATCY	2 cut(s)	919, 2059
MhlI	GDGCHC	2 cut(s)	31, 1813
Mph1103I	ATGCAT	3 cut(s)	519, 1084, 1990
MroI	TCCGGA	2 cut(s)	1327, 2154
MroXI	GAANNNNTTC	2 cut(s)	1698, 2166
MslI	CAYNNNNRTG	5 cut(s)	88, 570, 1146, 1395, 2041
MspA1I	CMGCKG	1 cut(s)	1583
MspCI	CTTAAG	1 cut(s)	1228
MspI	CCGG	4 cut(s)	342, 363, 1328, 2155
MspR9I	CCNGG	4 cut(s)	163, 342, 1512, 2018
MunI	CAATTG	2 cut(s)	756, 846
Mva1269I	GAATGC	1 cut(s)	1998
MvaI	CCWGG	3 cut(s)	163, 1512, 2018
MwoI	GCNNNNNNNGC	8 cut(s)	281, 290, 1439, 1448, 1451, 1460, 2108, 2190
NciI	CCSGG	1 cut(s)	342
NdeII	GATC	6 cut(s)	295, 894, 919, 2059, 2187, 2197
NheI	GCTAGC	2 cut(s)	868, 1519
NlaIV	GGNNCC	3 cut(s)	921, 1138, 2061
NmuCI	GTSAC	2 cut(s)	81, 727
NsiI	ATGCAT	3 cut(s)	519, 1084, 1990
NspI	RCATGY	3 cut(s)	569, 1244, 1263
NspV	TTCGAA	1 cut(s)	211
PaeI	GCATGC	1 cut(s)	569
PagI	TCATGA	2 cut(s)	891, 1009
PceI	AGGCCT	1 cut(s)	2016
PciI	ACATGT	1 cut(s)	1259
PctI	GAATGC	1 cut(s)	1998
PdmI	GAANNNNTTC	2 cut(s)	1698, 2166
PfeI	GAWTC	8 cut(s)	301, 786, 792, 1044, 1406, 1550, 1577, 1711
PkrI	GCNGC	9 cut(s)	197, 205, 271, 274, 1444, 1453, 1678, 2104, 2179
Ppu21I	YACGTR	1 cut(s)	1121
PscI	ACATGT	1 cut(s)	1259
PshBI	ATTAAT	1 cut(s)	1053
Psp124BI	GAGCTC	1 cut(s)	31
Psp6I	CCWGG	3 cut(s)	161, 1510, 2016
PspEI	GGTNACC	1 cut(s)	244
PspGI	CCWGG	3 cut(s)	161, 1510, 2016
PspN4I	GGNNCC	3 cut(s)	921, 1138, 2061
PspPI	GGNCC	1 cut(s)	339
PsuI	RGATCY	2 cut(s)	919, 2059
PvuII	CAGCTG	1 cut(s)	1583
RsaI	GTAC	3 cut(s)	916, 1123, 1401
RsaNI	GTAC	3 cut(s)	915, 1122, 1400
RseI	CAYNNNNRTG	5 cut(s)	88, 570, 1146, 1395, 2041
SacI	GAGCTC	1 cut(s)	31
SatI	GCNGC	9 cut(s)	196, 204, 270, 273, 1443, 1452, 1677, 2103, 2178
Sau3AI	GATC	6 cut(s)	295, 894, 919, 2059, 2187, 2197
Sau96I	GGNCC	1 cut(s)	339
ScrFI	CCNGG	4 cut(s)	163, 342, 1512, 2018
SduI	GDGCHC	2 cut(s)	31, 1813
SfaNI	GCATC	7 cut(s)	489, 526, 1069, 1783, 1997, 2019, 2089
SfcI	CTRYAG	1 cut(s)	601
SfuI	TTCGAA	1 cut(s)	211
SmiMI	CAYNNNNRTG	5 cut(s)	88, 570, 1146, 1395, 2041
SmlI	CTYRAG	3 cut(s)	423, 1228, 1436
SmoI	CTYRAG	3 cut(s)	423, 1228, 1436
SnaBI	TACGTA	1 cut(s)	1121
SpeI	ACTAGT	1 cut(s)	1471
SphI	GCATGC	1 cut(s)	569
SseBI	AGGCCT	1 cut(s)	2016
SsiI	CCGC	2 cut(s)	206, 272
SspMI	CTAG	8 cut(s)	39, 192, 401, 869, 1100, 1314, 1472, 1520
SstI	GAGCTC	1 cut(s)	31
StuI	AGGCCT	1 cut(s)	2016
StyD4I	CCNGG	4 cut(s)	161, 340, 1510, 2016
StyI	CCWWGG	1 cut(s)	1186
TaaI	ACNGT	3 cut(s)	550, 559, 1096
TaiI	ACGT	2 cut(s)	189, 1123
TaqI	TCGA	2 cut(s)	175, 211
TatI	WGTACW	1 cut(s)	1399
TauI	GCSGC	1 cut(s)	275
TfiI	GAWTC	8 cut(s)	301, 786, 792, 1044, 1406, 1550, 1577, 1711
TscAI	CASTG	2 cut(s)	1073, 1101
TseFI	GTSAC	2 cut(s)	81, 727
TseI	GCWGC	8 cut(s)	195, 203, 269, 1442, 1451, 1676, 2102, 2177
Tsp45I	GTSAC	2 cut(s)	81, 727
TspDTI	ATGAA	9 cut(s)	52, 67, 217, 368, 408, 957, 998, 1026, 1707
TspGWI	ACGGA	2 cut(s)	932, 1146
TspRI	CASTG	2 cut(s)	1073, 1101
Vha464I	CTTAAG	1 cut(s)	1228
VspI	ATTAAT	1 cut(s)	1053
XapI	RAATTY	3 cut(s)	761, 1647, 1698
XceI	RCATGY	3 cut(s)	569, 1244, 1263
XcmI	CCANNNNNNNNNTGG	1 cut(s)	848
XmnI	GAANNNNTTC	2 cut(s)	1698, 2166
XspI	CTAG	8 cut(s)	39, 192, 401, 869, 1100, 1314, 1472, 1520
Zsp2I	ATGCAT	3 cut(s)	519, 1084, 1990

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.