AT3G20230

Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004086
Physical Location & Seq
Reverse (-)
7055353 .. 7056847
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G20230.1

Sequence Viewer

Length: 564 bp
ATGTCTTGCTTGATTTCATCAACTTGCTCACTCGTCCCCTTCGACTCGAATCTGACTCGTTCGAATCGTTTCACCAAACTGGGTTCTGCAGTTTCATGGCAATCTTCCTTTCCTAAACTCTCCATCGAAATCGGCTCTGTTATCTCATCCCCCATTGTCAAGAAGGATTCTTTTGTGCAAGCTGCGTGGACTCGAAGATCACGAGGTGAAGCAGCGAAAAGACCCAACAGGAAGTCATGGAAACAGAGAACGGACATGTACATGAGGCCTTTCCTTCTGAATGTGTTCTTCTCCAGGAAATTCATACACGCTAAAGTGATGCATAGGCCAACAAGCAAAGTCATTTCAGTTGCCACCACAAATGCTAGAGATATAAGGACTAATATACCGTCTCTTGTTGATAACGAAGCGTGTAGACTCATCGGTAAGCTCATAGCTGAGCGTTCAATGGAAGCAGACGTCTATGCTGTCTCTTATGAGCCAAGAAAGGGAGAAAGAATTGAAGGGAAGCTAGGGATTGTAATCGATACTATTAAGGAACATGGCATTATTTTTGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

187

Amino Acids

21.12

Weight (kDa)

10.25

Isoelectric Point (pI)

55.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 85 - 185 2.3e-09 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 462
AccI GTMKAC 1 cut(s) 415
AcsI RAATTY 1 cut(s) 299
AcyI GRCGYC 1 cut(s) 459
AdeI CACNNNGTG 1 cut(s) 206
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 1 cut(s) 488
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 2 cut(s) 447, 503
AjnI CCWGG 1 cut(s) 293
AluBI AGCT 4 cut(s) 182, 430, 437, 511
AluI AGCT 4 cut(s) 182, 430, 437, 511
Alw26I GTCTC 2 cut(s) 396, 475
AoxI GGCC 2 cut(s) 266, 326
ApeKI GCWGC 2 cut(s) 182, 212
ApoI RAATTY 1 cut(s) 299
Asp700I GAANNNNTTC 2 cut(s) 68, 284
AsuHPI GGTGA 2 cut(s) 64, 218
AsuII TTCGAA 1 cut(s) 62
BauI CACGAG 1 cut(s) 201
BbvI GCAGC 2 cut(s) 169, 224
BccI CCATC 1 cut(s) 131
BciT130I CCWGG 1 cut(s) 295
BcoDI GTCTC 2 cut(s) 396, 475
BfaI CTAG 2 cut(s) 366, 512
BfmI CTRYAG 1 cut(s) 87
BisI GCNGC 2 cut(s) 183, 213
BlpI GCTNAGC 1 cut(s) 438
BlsI GCNGC 2 cut(s) 184, 214
Bme1390I CCNGG 1 cut(s) 295
BmrFI CCNGG 1 cut(s) 295
BmrI ACTGGG 1 cut(s) 89
BmsI GCATC 1 cut(s) 309
BmuI ACTGGG 1 cut(s) 89
BpmI CTGGAG 1 cut(s) 277
Bpu1102I GCTNAGC 1 cut(s) 438
Bpu14I TTCGAA 1 cut(s) 62
Bsa29I ATCGAT 1 cut(s) 525
BsaBI GATNNNNATC 1 cut(s) 521
BsaHI GRCGYC 1 cut(s) 459
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse1I ACTGG 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 521
BseBI CCWGG 1 cut(s) 295
BseCI ATCGAT 1 cut(s) 525
BseGI GGATG 1 cut(s) 146
BseJI GATNNNNATC 1 cut(s) 521
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMII CTCAG 1 cut(s) 429
BseNI ACTGG 1 cut(s) 84
BseXI GCAGC 2 cut(s) 169, 224
BshFI GGCC 2 cut(s) 268, 328
BshVI ATCGAT 1 cut(s) 525
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 488
BsmAI GTCTC 2 cut(s) 396, 475
BsmBI CGTCTC 1 cut(s) 396
BsmFI GGGAC 1 cut(s) 20
BsnI GGCC 2 cut(s) 268, 328
Bsp119I TTCGAA 1 cut(s) 62
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 1 cut(s) 197
Bsp1720I GCTNAGC 1 cut(s) 438
BspANI GGCC 2 cut(s) 268, 328
BspCNI CTCAG 1 cut(s) 430
BspDI ATCGAT 1 cut(s) 525
BspMAI CTGCAG 1 cut(s) 91
BspT104I TTCGAA 1 cut(s) 62
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 1 cut(s) 84
BssMI GATC 1 cut(s) 197
BssNI GRCGYC 1 cut(s) 459
BssSI CACGAG 1 cut(s) 201
Bst2BI CACGAG 1 cut(s) 201
Bst2UI CCWGG 1 cut(s) 295
Bst4CI ACNGT 1 cut(s) 390
BstACI GRCGYC 1 cut(s) 459
BstAUI TGTACA 1 cut(s) 258
BstBI TTCGAA 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 180
BstDEI CTNAG 1 cut(s) 438
BstF5I GGATG 1 cut(s) 146
BstKTI GATC 1 cut(s) 200
BstMAI GTCTC 2 cut(s) 396, 475
BstMBI GATC 1 cut(s) 197
BstNI CCWGG 1 cut(s) 295
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 293
BstSFI CTRYAG 1 cut(s) 87
BstV1I GCAGC 2 cut(s) 169, 224
Bsu15I ATCGAT 1 cut(s) 525
BsuRI GGCC 2 cut(s) 268, 328
BsuTUI ATCGAT 1 cut(s) 525
BtsCI GGATG 1 cut(s) 146
Cac8I GCNNGC 1 cut(s) 180
ClaI ATCGAT 1 cut(s) 525
Csp6I GTAC 1 cut(s) 259
CspCI CAANNNNNGTGG 2 cut(s) 167, 202
CviAII CATG 5 cut(s) 96, 237, 256, 262, 542
CviJI RGCY 8 cut(s) 135, 182, 268, 328, 430, 437, 481, 511
CviKI_1 RGCY 8 cut(s) 135, 182, 268, 328, 430, 437, 481, 511
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 1 cut(s) 438
DpnI GATC 1 cut(s) 199
DpnII GATC 1 cut(s) 197
DraIII CACNNNGTG 1 cut(s) 206
Eco147I AGGCCT 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 293
EcoT22I ATGCAT 1 cut(s) 324
Esp3I CGTCTC 1 cut(s) 396
FaeI CATG 5 cut(s) 99, 240, 259, 265, 545
FaqI GGGAC 1 cut(s) 20
FatI CATG 5 cut(s) 95, 236, 255, 261, 541
FblI GTMKAC 1 cut(s) 415
Fnu4HI GCNGC 2 cut(s) 183, 213
FokI GGATG 1 cut(s) 133
Fsp4HI GCNGC 2 cut(s) 183, 213
FspBI CTAG 2 cut(s) 366, 512
GluI GCNGC 2 cut(s) 183, 213
GsuI CTGGAG 1 cut(s) 277
HaeIII GGCC 2 cut(s) 268, 328
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 5 cut(s) 99, 240, 259, 265, 545
HinfI GANTC 7 cut(s) 44, 49, 55, 64, 167, 190, 417
HphI GGTGA 2 cut(s) 64, 218
Hpy166II GTNNAC 2 cut(s) 189, 416
Hpy188I TCNGA 2 cut(s) 54, 279
Hpy188III TCNNGA 2 cut(s) 160, 201
Hpy8I GTNNAC 2 cut(s) 189, 416
HpyAV CCTTC 4 cut(s) 49, 157, 284, 497
HpyCH4III ACNGT 1 cut(s) 390
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 3 cut(s) 89, 178, 322
HpyF3I CTNAG 1 cut(s) 438
HpySE526I ACGT 1 cut(s) 459
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 5 cut(s) 99, 240, 259, 265, 545
Kzo9I GATC 1 cut(s) 197
LpnPI CCDG 4 cut(s) 65, 214, 280, 307
Lsp1109I GCAGC 2 cut(s) 169, 224
LweI GCATC 1 cut(s) 309
MaeI CTAG 2 cut(s) 366, 512
MaeII ACGT 1 cut(s) 459
MalI GATC 1 cut(s) 199
MboI GATC 1 cut(s) 197
MboII GAAGA 3 cut(s) 96, 207, 280
MluCI AATT 2 cut(s) 299, 498
MlyI GAGTC 4 cut(s) 38, 49, 184, 411
MnlI CCTC 2 cut(s) 197, 258
Mph1103I ATGCAT 1 cut(s) 324
MroXI GAANNNNTTC 2 cut(s) 68, 284
MseI TTAA 1 cut(s) 534
MslI CAYNNNNRTG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 295
MvaI CCWGG 1 cut(s) 295
NdeII GATC 1 cut(s) 197
NlaIII CATG 5 cut(s) 99, 240, 259, 265, 545
NsiI ATGCAT 1 cut(s) 324
NspI RCATGY 1 cut(s) 259
NspV TTCGAA 1 cut(s) 62
PceI AGGCCT 1 cut(s) 268
PciI ACATGT 1 cut(s) 255
PcsI WCGNNNNNNNCGW 3 cut(s) 39, 64, 199
PdmI GAANNNNTTC 2 cut(s) 68, 284
PfeI GAWTC 3 cut(s) 49, 64, 167
PfoI TCCNGGA 1 cut(s) 293
PkrI GCNGC 2 cut(s) 184, 214
PleI GAGTC 4 cut(s) 38, 49, 184, 411
PpsI GAGTC 4 cut(s) 38, 49, 184, 411
PscI ACATGT 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 293
PspGI CCWGG 1 cut(s) 293
PstI CTGCAG 1 cut(s) 91
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
RseI CAYNNNNRTG 1 cut(s) 260
SaqAI TTAA 1 cut(s) 534
SatI GCNGC 2 cut(s) 183, 213
Sau3AI GATC 1 cut(s) 197
SchI GAGTC 4 cut(s) 38, 49, 184, 411
ScrFI CCNGG 1 cut(s) 295
SetI ASST 6 cut(s) 184, 208, 432, 439, 462, 513
SfaNI GCATC 1 cut(s) 309
SfcI CTRYAG 1 cut(s) 87
SfuI TTCGAA 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 260
Sse9I AATT 2 cut(s) 299, 498
SseBI AGGCCT 1 cut(s) 268
SspMI CTAG 2 cut(s) 366, 512
StuI AGGCCT 1 cut(s) 268
StyD4I CCNGG 1 cut(s) 293
TaaI ACNGT 1 cut(s) 390
TaiI ACGT 1 cut(s) 462
TaqI TCGA 6 cut(s) 42, 47, 62, 126, 193, 525
TasI AATT 2 cut(s) 299, 498
TatI WGTACW 1 cut(s) 258
TfiI GAWTC 3 cut(s) 49, 64, 167
Tru1I TTAA 1 cut(s) 534
Tru9I TTAA 1 cut(s) 534
TseI GCWGC 2 cut(s) 182, 212
TspDTI ATGAA 3 cut(s) 6, 84, 292
TspGWI ACGGA 1 cut(s) 266
XapI RAATTY 1 cut(s) 299
XceI RCATGY 1 cut(s) 259
XmiI GTMKAC 1 cut(s) 415
XmnI GAANNNNTTC 2 cut(s) 68, 284
XspI CTAG 2 cut(s) 366, 512
ZraI GACGTC 1 cut(s) 460
Zsp2I ATGCAT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.