RLG00000028962

ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004086
Physical Location & Seq
Reverse (-)
30746384 .. 30747897
1514 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028962

Sequence Viewer

Length: 531 bp
ATGGGTTGCATAGCTCCTCCCTCATGTGGTTCGCTAAGACCCACCCAGCTTTCGTGGGCATCTTCTTTTCCGAAGCCTCCCATCTCCTTCAATTGCACCACAACCCCTCCCAATCTCATTCACTCACCCAACAAGGTTTCCTTCGTTCGAGCTGCATGGACCCGGAGATCTCGAGGCGAGGCTGCAAAAAGACCTAATAAGAAATCATGGGGTCAACGGACAGATATGTTCCTAAGGCCATTTTTACTGGATGTTTTCTTTTCAAAACGATTTATCCATGCCAAAGTGATGCACCGCGGTACCAGCAAAGTAATATCTGTGGCAAGTACAAACGCCAAGGATTTGAGATTTGCATTGCCGTCGCTTACTGATGACAATGCTTGTCGAGTTATAGGAAACCTGATTGCAGAGAGGTCCAAGGATGCTGATGTATTTGCGATGTCTTTTGAGCCCAAAAAGAATGAGAAGATTGAGGGTAGGCTGGGACTTGTTCTTGATACCATTAAAGAGAACGGTATAATCTTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

177

Amino Acids

19.62

Weight (kDa)

10.25

Isoelectric Point (pI)

51.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 79 - 175 4.6e-09 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 299
AccB1I GGYRCC 1 cut(s) 299
AccII CGCG 1 cut(s) 297
AciI CCGC 2 cut(s) 295, 297
AfaI GTAC 2 cut(s) 301, 328
AfiI CCNNNNNNNGG 1 cut(s) 26
AgsI TTSAA 2 cut(s) 91, 264
AluBI AGCT 3 cut(s) 14, 49, 152
AluI AGCT 3 cut(s) 14, 49, 152
Ama87I CYCGRG 1 cut(s) 171
AoxI GGCC 1 cut(s) 236
ApeKI GCWGC 2 cut(s) 152, 182
Asp718I GGTACC 1 cut(s) 299
AspS9I GGNCC 2 cut(s) 159, 414
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 1 cut(s) 117
AvaI CYCGRG 1 cut(s) 171
AvaII GGWCC 2 cut(s) 159, 414
AxyI CCTNAGG 1 cut(s) 233
BaeI ACNNNNGTAYC 2 cut(s) 283, 316
BanI GGYRCC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 453
BbvI GCAGC 2 cut(s) 139, 169
BccI CCATC 1 cut(s) 89
BceAI ACGGC 1 cut(s) 343
BcgI CGANNNNNNTGC 2 cut(s) 342, 376
BcnI CCSGG 1 cut(s) 163
BglII AGATCT 1 cut(s) 167
BisI GCNGC 2 cut(s) 153, 183
BlsI GCNGC 2 cut(s) 154, 184
Bme1390I CCNGG 1 cut(s) 163
Bme18I GGWCC 2 cut(s) 159, 414
BmeT110I CYCGRG 1 cut(s) 171
BmgT120I GGNCC 2 cut(s) 159, 414
BmiI GGNNCC 2 cut(s) 161, 301
BmrFI CCNGG 1 cut(s) 163
BmsI GCATC 3 cut(s) 68, 279, 412
BpuMI CCSGG 1 cut(s) 163
BsaJI CCNNGG 3 cut(s) 295, 336, 417
BsaXI ACNNNNNCTCC 2 cut(s) 91, 121
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 252
Bse21I CCTNAGG 1 cut(s) 233
Bse3DI GCAATG 1 cut(s) 353
BseDI CCNNGG 3 cut(s) 295, 336, 417
BseGI GGATG 2 cut(s) 256, 427
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMI GCAATG 1 cut(s) 353
BseNI ACTGG 1 cut(s) 252
BseRI GAGGAG 1 cut(s) 6
BseXI GCAGC 2 cut(s) 139, 169
BseYI CCCAGC 2 cut(s) 45, 481
Bsh1236I CGCG 1 cut(s) 297
BshFI GGCC 1 cut(s) 238
BshNI GGYRCC 1 cut(s) 299
BsiHKCI CYCGRG 1 cut(s) 171
BsiSI CCGG 1 cut(s) 163
BslFI GGGAC 1 cut(s) 498
BslI CCNNNNNNNGG 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 498
BsnI GGCC 1 cut(s) 238
BsoBI CYCGRG 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 453
Bsp143I GATC 1 cut(s) 167
BspACI CCGC 2 cut(s) 295, 297
BspANI GGCC 1 cut(s) 238
BspFNI CGCG 1 cut(s) 297
BspLI GGNNCC 2 cut(s) 161, 301
BspT107I GGYRCC 1 cut(s) 299
BsrDI GCAATG 1 cut(s) 353
BsrI ACTGG 1 cut(s) 252
BssECI CCNNGG 3 cut(s) 295, 336, 417
BssMI GATC 1 cut(s) 167
BssT1I CCWWGG 2 cut(s) 336, 417
Bst4CI ACNGT 1 cut(s) 515
BstDEI CTNAG 2 cut(s) 35, 233
BstDSI CCRYGG 1 cut(s) 295
BstF5I GGATG 2 cut(s) 256, 427
BstFNI CGCG 1 cut(s) 297
BstKTI GATC 1 cut(s) 170
BstMBI GATC 1 cut(s) 167
BstMWI GCNNNNNNNGC 1 cut(s) 303
BstSCI CCNGG 1 cut(s) 161
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 2 cut(s) 139, 169
BstX2I RGATCY 1 cut(s) 167
BstYI RGATCY 1 cut(s) 167
Bsu36I CCTNAGG 1 cut(s) 233
BsuRI GGCC 1 cut(s) 238
BtgI CCRYGG 1 cut(s) 295
BtgZI GCGATG 1 cut(s) 452
BtsCI GGATG 2 cut(s) 256, 427
Cfr13I GGNCC 2 cut(s) 159, 414
Cfr42I CCGCGG 1 cut(s) 298
Csp6I GTAC 2 cut(s) 300, 327
CviAII CATG 4 cut(s) 24, 156, 207, 278
CviJI RGCY 8 cut(s) 14, 49, 76, 152, 182, 238, 451, 481
CviKI_1 RGCY 8 cut(s) 14, 49, 76, 152, 182, 238, 451, 481
CviQI GTAC 2 cut(s) 300, 327
DdeI CTNAG 2 cut(s) 35, 233
DpnI GATC 1 cut(s) 169
DpnII GATC 1 cut(s) 167
Eco130I CCWWGG 2 cut(s) 336, 417
Eco24I GRGCYC 1 cut(s) 453
Eco47I GGWCC 2 cut(s) 159, 414
Eco81I CCTNAGG 1 cut(s) 233
Eco88I CYCGRG 1 cut(s) 171
EcoT14I CCWWGG 2 cut(s) 336, 417
EcoT38I GRGCYC 1 cut(s) 453
ErhI CCWWGG 2 cut(s) 336, 417
FaeI CATG 4 cut(s) 27, 159, 210, 281
FaiI YATR 8 cut(s) 11, 25, 157, 208, 227, 279, 392, 518
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FaqI GGGAC 1 cut(s) 498
FatI CATG 4 cut(s) 23, 155, 206, 277
Fnu4HI GCNGC 2 cut(s) 153, 183
FokI GGATG 2 cut(s) 263, 434
FriOI GRGCYC 1 cut(s) 453
Fsp4HI GCNGC 2 cut(s) 153, 183
GluI GCNGC 2 cut(s) 153, 183
GsaI CCCAGC 2 cut(s) 49, 485
HaeIII GGCC 1 cut(s) 238
HapII CCGG 1 cut(s) 163
Hin1II CATG 4 cut(s) 27, 159, 210, 281
HincII GTYRAC 1 cut(s) 215
HindII GTYRAC 1 cut(s) 215
HpaII CCGG 1 cut(s) 163
HphI GGTGA 1 cut(s) 117
Hpy166II GTNNAC 1 cut(s) 215
Hpy188I TCNGA 1 cut(s) 72
Hpy188III TCNNGA 2 cut(s) 171, 494
Hpy8I GTNNAC 1 cut(s) 215
Hpy99I CGWCG 1 cut(s) 364
HpyAV CCTTC 2 cut(s) 97, 151
HpyCH4III ACNGT 1 cut(s) 515
HpyCH4V TGCA 7 cut(s) 9, 96, 155, 185, 292, 353, 407
HpyF10VI GCNNNNNNNGC 1 cut(s) 303
HpyF3I CTNAG 2 cut(s) 35, 233
Hsp92II CATG 4 cut(s) 27, 159, 210, 281
KpnI GGTACC 1 cut(s) 303
KspI CCGCGG 1 cut(s) 298
Kzo9I GATC 1 cut(s) 167
LmnI GCTCC 1 cut(s) 19
LpnPI CCDG 6 cut(s) 59, 176, 233, 316, 413, 467
Lsp1109I GCAGC 2 cut(s) 139, 169
LweI GCATC 3 cut(s) 68, 279, 412
MalI GATC 1 cut(s) 169
MboI GATC 1 cut(s) 167
MboII GAAGA 2 cut(s) 54, 478
MfeI CAATTG 1 cut(s) 91
MflI RGATCY 1 cut(s) 167
MhlI GDGCHC 1 cut(s) 453
MluCI AATT 1 cut(s) 91
MnlI CCTC 8 cut(s) 27, 31, 87, 117, 167, 172, 405, 466
MseI TTAA 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 297
MspI CCGG 1 cut(s) 163
MspR9I CCNGG 1 cut(s) 163
MunI CAATTG 1 cut(s) 91
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 1 cut(s) 303
NciI CCSGG 1 cut(s) 163
NdeII GATC 1 cut(s) 167
NlaIII CATG 4 cut(s) 27, 159, 210, 281
NlaIV GGNNCC 2 cut(s) 161, 301
PaeR7I CTCGAG 1 cut(s) 171
PkrI GCNGC 2 cut(s) 154, 184
PspFI CCCAGC 2 cut(s) 45, 481
PspN4I GGNNCC 2 cut(s) 161, 301
PspPI GGNCC 2 cut(s) 159, 414
PsuI RGATCY 1 cut(s) 167
RsaI GTAC 2 cut(s) 301, 328
RsaNI GTAC 2 cut(s) 300, 327
SacII CCGCGG 1 cut(s) 298
SaqAI TTAA 1 cut(s) 504
SatI GCNGC 2 cut(s) 153, 183
Sau3AI GATC 1 cut(s) 167
Sau96I GGNCC 2 cut(s) 159, 414
ScrFI CCNGG 1 cut(s) 163
SduI GDGCHC 1 cut(s) 453
SetI ASST 7 cut(s) 16, 51, 138, 154, 196, 402, 416
SfaNI GCATC 3 cut(s) 68, 279, 412
Sfr274I CTCGAG 1 cut(s) 171
Sfr303I CCGCGG 1 cut(s) 298
SgrBI CCGCGG 1 cut(s) 298
SinI GGWCC 2 cut(s) 159, 414
SlaI CTCGAG 1 cut(s) 171
SmlI CTYRAG 1 cut(s) 171
SmoI CTYRAG 1 cut(s) 171
Sse9I AATT 1 cut(s) 91
SsiI CCGC 2 cut(s) 295, 297
StyD4I CCNGG 1 cut(s) 161
StyI CCWWGG 2 cut(s) 336, 417
TaaI ACNGT 1 cut(s) 515
TaqI TCGA 3 cut(s) 148, 172, 385
TasI AATT 1 cut(s) 91
TatI WGTACW 1 cut(s) 326
Tru1I TTAA 1 cut(s) 504
Tru9I TTAA 1 cut(s) 504
TseI GCWGC 2 cut(s) 152, 182
TspGWI ACGGA 1 cut(s) 232
VpaK11BI GGWCC 2 cut(s) 159, 414
XhoI CTCGAG 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.