pycom07g06940

ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0004086
Physical Location & Seq
Forward (+)
6294863 .. 6296528
1666 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g06940.1

Sequence Viewer

Length: 564 bp
ATGGCCTGCTGCACATCAGCTCCTCCTCTGCCGCCGCCGCTGTCAAACCCCTGTGGAAGTGGTTCGGTGAAACCCAGCCGGCTCTCATGGGCATCTTCCTTTCCCAACTTCGGCGTCTCCTTCAATTGCACCACAAGCCCTCCTAATCGCATTCACACCCCCAACAAGGATTCCTTCATTCGAGCTGCATGGACCCGGAGATCTCGAGGCGAGGCTGCAAATAGACCTAATAGGAAATCATGGAGTCAAAGGACAGATATGTACATGAGGCCATTTTTACTGGATGTTTTCTTTTCAAAACGATTCATCCATGCCAAAGTGATGCACAGGGGTACCAGCAAAGTGATATCCGTGGCCAGCACAAACTCCAAGGATTTGAGGTATTCGTTGCCATCGCTTACCGATAACGAAGCATGTGTAGTTGTAGGAAACCTGATTGCAGAGAGGTGCAAGGATGCCGATGTGTTTGCAATGTCTTTTGAACCCAAAAAGAATGAGAGGATTGAGGGTAGGCTTGGAATCGTTCTCGATACCATTAAGGATAATGGCATCATATTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L18p

Protein Analysis

188

Amino Acids

20.81

Weight (kDa)

9.88

Isoelectric Point (pI)

49.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 89 - 186 4e-08 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 332
AccB1I GGYRCC 1 cut(s) 332
AciI CCGC 3 cut(s) 32, 35, 38
AcoI YGGCCR 1 cut(s) 354
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 2 cut(s) 263, 334
AfiI CCNNNNNNNGG 2 cut(s) 110, 166
AgsI TTSAA 3 cut(s) 124, 297, 482
AluBI AGCT 2 cut(s) 20, 185
AluI AGCT 2 cut(s) 20, 185
Alw26I GTCTC 1 cut(s) 121
Ama87I CYCGRG 1 cut(s) 204
AoxI GGCC 3 cut(s) 3, 269, 354
ApeKI GCWGC 3 cut(s) 9, 185, 215
Asp700I GAANNNNTTC 1 cut(s) 61
Asp718I GGTACC 1 cut(s) 332
AspS9I GGNCC 1 cut(s) 192
AsuC2I CCSGG 1 cut(s) 196
AsuHPI GGTGA 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 204
AvaII GGWCC 1 cut(s) 192
BaeI ACNNNNGTAYC 2 cut(s) 316, 349
BalI TGGCCA 1 cut(s) 356
BanI GGYRCC 1 cut(s) 332
BbvI GCAGC 2 cut(s) 172, 202
BccI CCATC 1 cut(s) 400
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BcnI CCSGG 1 cut(s) 196
BcoDI GTCTC 1 cut(s) 121
BglII AGATCT 1 cut(s) 200
BisI GCNGC 6 cut(s) 10, 32, 35, 38, 186, 216
BlsI GCNGC 6 cut(s) 11, 33, 36, 39, 187, 217
Bme1390I CCNGG 1 cut(s) 196
Bme18I GGWCC 1 cut(s) 192
BmeT110I CYCGRG 1 cut(s) 204
BmgT120I GGNCC 1 cut(s) 192
BmiI GGNNCC 2 cut(s) 194, 334
BmrFI CCNGG 1 cut(s) 196
BmsI GCATC 4 cut(s) 101, 312, 445, 558
BpuMI CCSGG 1 cut(s) 196
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 2 cut(s) 351, 369
BsaXI ACNNNNNCTCC 3 cut(s) 34, 124, 154
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 166
Bse118I RCCGGY 1 cut(s) 78
Bse1I ACTGG 1 cut(s) 285
Bse3DI GCAATG 1 cut(s) 477
BseDI CCNNGG 2 cut(s) 351, 369
BseGI GGATG 3 cut(s) 289, 306, 460
BseLI CCNNNNNNNGG 2 cut(s) 110, 166
BseMI GCAATG 1 cut(s) 477
BseNI ACTGG 1 cut(s) 285
BseRI GAGGAG 2 cut(s) 12, 15
BseXI GCAGC 2 cut(s) 172, 202
BseYI CCCAGC 1 cut(s) 74
BshFI GGCC 3 cut(s) 5, 271, 356
BshNI GGYRCC 1 cut(s) 332
BsiHKCI CYCGRG 1 cut(s) 204
BsiSI CCGG 2 cut(s) 79, 196
BslI CCNNNNNNNGG 2 cut(s) 110, 166
BsmAI GTCTC 1 cut(s) 121
BsmBI CGTCTC 1 cut(s) 121
BsmI GAATGC 1 cut(s) 150
BsnI GGCC 3 cut(s) 5, 271, 356
BsoBI CYCGRG 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 261
Bsp143I GATC 1 cut(s) 200
BspACI CCGC 3 cut(s) 32, 35, 38
BspANI GGCC 3 cut(s) 5, 271, 356
BspLI GGNNCC 2 cut(s) 194, 334
BspT107I GGYRCC 1 cut(s) 332
BsrDI GCAATG 1 cut(s) 477
BsrFI RCCGGY 1 cut(s) 78
BsrGI TGTACA 1 cut(s) 261
BsrI ACTGG 1 cut(s) 285
BssAI RCCGGY 1 cut(s) 78
BssECI CCNNGG 2 cut(s) 351, 369
BssMI GATC 1 cut(s) 200
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 1 cut(s) 369
BstACI GRCGYC 1 cut(s) 114
BstAUI TGTACA 1 cut(s) 261
BstC8I GCNNGC 3 cut(s) 7, 80, 358
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 3 cut(s) 289, 306, 460
BstKTI GATC 1 cut(s) 203
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 1 cut(s) 200
BstMWI GCNNNNNNNGC 2 cut(s) 37, 135
BstNSI RCATGY 1 cut(s) 417
BstSCI CCNGG 1 cut(s) 194
BstV1I GCAGC 2 cut(s) 172, 202
BstX2I RGATCY 1 cut(s) 200
BstYI RGATCY 1 cut(s) 200
BsuRI GGCC 3 cut(s) 5, 271, 356
BtgI CCRYGG 1 cut(s) 351
BtgZI GCGATG 1 cut(s) 378
BtsCI GGATG 3 cut(s) 289, 306, 460
Cac8I GCNNGC 3 cut(s) 7, 80, 358
Cfr10I RCCGGY 1 cut(s) 78
Cfr13I GGNCC 1 cut(s) 192
CseI GACGC 1 cut(s) 103
Csp6I GTAC 2 cut(s) 262, 333
CviAII CATG 6 cut(s) 87, 189, 240, 265, 311, 414
CviQI GTAC 2 cut(s) 262, 333
DpnI GATC 1 cut(s) 202
DpnII GATC 1 cut(s) 200
EaeI YGGCCR 1 cut(s) 354
Eco130I CCWWGG 1 cut(s) 369
Eco32I GATATC 1 cut(s) 348
Eco47I GGWCC 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 204
EcoRV GATATC 1 cut(s) 348
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 6 cut(s) 90, 192, 243, 268, 314, 417
FaiI YATR 8 cut(s) 88, 190, 241, 260, 266, 312, 415, 554
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FatI CATG 6 cut(s) 86, 188, 239, 264, 310, 413
Fnu4HI GCNGC 6 cut(s) 10, 32, 35, 38, 186, 216
FokI GGATG 3 cut(s) 293, 296, 467
Fsp4HI GCNGC 6 cut(s) 10, 32, 35, 38, 186, 216
GluI GCNGC 6 cut(s) 10, 32, 35, 38, 186, 216
GsaI CCCAGC 1 cut(s) 78
HaeIII GGCC 3 cut(s) 5, 271, 356
HapII CCGG 2 cut(s) 79, 196
HgaI GACGC 1 cut(s) 103
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 6 cut(s) 90, 192, 243, 268, 314, 417
HinfI GANTC 4 cut(s) 170, 244, 303, 519
HpaII CCGG 2 cut(s) 79, 196
HphI GGTGA 1 cut(s) 79
Hpy188III TCNNGA 2 cut(s) 204, 527
HpyAV CCTTC 2 cut(s) 130, 184
HpyCH4V TGCA 8 cut(s) 12, 129, 188, 218, 325, 440, 450, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 37, 135
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 6 cut(s) 90, 192, 243, 268, 314, 417
KpnI GGTACC 1 cut(s) 336
KroI GCCGGC 1 cut(s) 78
KroNI GCCGGC 1 cut(s) 80
Kzo9I GATC 1 cut(s) 200
LmnI GCTCC 1 cut(s) 25
Lsp1109I GCAGC 2 cut(s) 172, 202
LweI GCATC 4 cut(s) 101, 312, 445, 558
MalI GATC 1 cut(s) 202
MboI GATC 1 cut(s) 200
MboII GAAGA 1 cut(s) 87
MfeI CAATTG 1 cut(s) 124
MflI RGATCY 1 cut(s) 200
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 1 cut(s) 124
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 1 cut(s) 253
Mox20I TGGCCA 1 cut(s) 356
MroNI GCCGGC 1 cut(s) 78
MroXI GAANNNNTTC 1 cut(s) 61
MscI TGGCCA 1 cut(s) 356
MseI TTAA 1 cut(s) 537
Msp20I TGGCCA 1 cut(s) 356
MspA1I CMGCKG 1 cut(s) 40
MspI CCGG 2 cut(s) 79, 196
MspR9I CCNGG 1 cut(s) 196
MunI CAATTG 1 cut(s) 124
Mva1269I GAATGC 1 cut(s) 150
MwoI GCNNNNNNNGC 2 cut(s) 37, 135
NaeI GCCGGC 1 cut(s) 80
NciI CCSGG 1 cut(s) 196
NdeII GATC 1 cut(s) 200
NgoMIV GCCGGC 1 cut(s) 78
NlaIII CATG 6 cut(s) 90, 192, 243, 268, 314, 417
NlaIV GGNNCC 2 cut(s) 194, 334
NspI RCATGY 1 cut(s) 417
PaeR7I CTCGAG 1 cut(s) 204
PctI GAATGC 1 cut(s) 150
PdiI GCCGGC 1 cut(s) 80
PdmI GAANNNNTTC 1 cut(s) 61
PfeI GAWTC 3 cut(s) 170, 303, 519
PkrI GCNGC 6 cut(s) 11, 33, 36, 39, 187, 217
PleI GAGTC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 252
PspFI CCCAGC 1 cut(s) 74
PspN4I GGNNCC 2 cut(s) 194, 334
PspPI GGNCC 1 cut(s) 192
PsuI RGATCY 1 cut(s) 200
RsaI GTAC 2 cut(s) 263, 334
RsaNI GTAC 2 cut(s) 262, 333
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 6 cut(s) 10, 32, 35, 38, 186, 216
Sau3AI GATC 1 cut(s) 200
Sau96I GGNCC 1 cut(s) 192
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 1 cut(s) 196
SetI ASST 6 cut(s) 22, 187, 229, 383, 435, 449
SfaNI GCATC 4 cut(s) 101, 312, 445, 558
Sfr274I CTCGAG 1 cut(s) 204
SinI GGWCC 1 cut(s) 192
SlaI CTCGAG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 204
SmoI CTYRAG 1 cut(s) 204
Sse9I AATT 1 cut(s) 124
SsiI CCGC 3 cut(s) 32, 35, 38
StyD4I CCNGG 1 cut(s) 194
StyI CCWWGG 1 cut(s) 369
TaqI TCGA 3 cut(s) 181, 205, 528
TasI AATT 1 cut(s) 124
TatI WGTACW 1 cut(s) 261
TauI GCSGC 3 cut(s) 34, 37, 40
TfiI GAWTC 3 cut(s) 170, 303, 519
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TseI GCWGC 3 cut(s) 9, 185, 215
TspDTI ATGAA 2 cut(s) 166, 295
TspGWI ACGGA 1 cut(s) 340
VpaK11BI GGWCC 1 cut(s) 192
XceI RCATGY 1 cut(s) 417
XhoI CTCGAG 1 cut(s) 204
XmnI GAANNNNTTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.