AT3G43920

Ribonuclease-III-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001909
Physical Location & Seq
Forward (+)
15753387 .. 15760859
7473 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G43920.1

Sequence Viewer

Length: 4596 bp
ATGCATTCGTCGTTGGAGCCGGAGAAAATGGAGGAAGGTGGGGGAAGCAATTCGCTTAAGAGAAAATTCTCTGAAATCGATGGAGATCAAAATCTTGATTCTGTCTCTTCTCCTATGATGACTGACTCTAATGGTAGTTATGAATTGAAAGTGTACGAGGTTGCTAAGAACAGGAACATAATTGCTGTTTTGGGGACAGGGATTGATAAGTCAGAGATCACTAAGAGGCTTATCAAAGCTATGGGTTCTTCTGATACAGACAAAAGATTGATAATTTTCTTGGCCCCAACTGTGAATCTTCAATGCTGTGAGATCAGAGCACTTGTGAATTTGAAAGTTGAAGAGTACTTTGGAGCTAAAGGAGTTGATAAATGGACATCTCAGCGCTGGGATGAGGAATTTAGCAAGCACGATGTTTTAGTTATGACTCCTCAAATATTATTGGATGTCCTTAGAAGTGCATTCCTGAAACTAGAGATGGTATGTCTTCTAATAATAGATGAATGCCACCATACCACTGGCAATCATCCCTATGCGAAGTTAATGAAGATTTTTAATCCTGAAGAGCGTGAAGGAGTGGAAAAGTTTGCTACAACGGTTAAAGAAGGTCCAATATTGTATAACCCATCACCATCCTGTAGTTTGGAATTGAAAGAAAAGTTAGAAACTTCACACCTCAAGTTTGATGCTTCTCTTAGAAGGCTTCAAGAGTTGGGAAAAGACAGTTTTCTGAATATGGATAATAAGTTTGAGACATATCAAAAGAGATTGTCTATCGACTACAGAGAGATTTTGCATTGCCTTGATAATCTTGGCCTGATTTGCGCACACTTGGCGGCTGAAGTCTGCTTGGAGAAAATCTCAGATACGAAAGAGGAAAGTGAAACTTATAAAGAATGCTCAATGGTGTGCAAGGAATTTCTTGAGGATATTTTATCCACCATTGGGGTGTATTTGCCGCAAGATGATAAGAGTCTGGTAGATTTGCAGCAAAACCATCTGTCAGCAGTAATTTCTGGGCATGTATCTCCAAAGCTAAAAGAACTCTTCCATCTATTGGATTCCTTTAGAGGTGACAAGCAAAAGCAGTGCCTTATTTTAGTTGAGAGAATTATAACTGCGAAAGTGATCGAAAGATTCGTTAAGAAAGAAGCCTCTTTGGCTTACCTTAATGTCTTGTATTTAACCGAAAACAACCCCTCCACCAATGTATCGGCACAGAAAATGCAAATTGAAATCCCTGATTTATTTCAACATGGCAAGGTGAATCTTTTATTCATCACAGATGTGGTTGAAGAGGGATTTCAGGTTCCAGATTGCTCATGCATGGTTTGTTTTGACCTGCCCAAAACAATGTGTAGTTACTCGCAGTCTCAAAAACATGCCAAACAGAGTAATTCTAAGTCTATCATGTTTCTTGAAAGAGGGAACCCGAAGCAAAGAGACCATCTGCATGACCTTATGCGAAGAGAAGTCCTAATTCAAGATCCAGAAGCTCCAAACTTGAAATCGTGTCCACCTCCAGTGAAAAATGGACACGGTGTGAAGGAGATTGGATCCATGGTTATCCCAGATTCTAACATAACTGTATCTGAGGAAGCAGCTTCCACACAAACTATGAGTGATCCTCCTAGCAGAAATGAGCAGTTACCACCGTGTAAAAAGTTACGCTTGGATAACAATCTCTTACAATCCAACGGCAAAGAGAAGGTTGCCTCTTCTAAAAGTAAATCATCTTCATCGGCTGCAGGTTCAAAAAAACGTAAGGAGTTGCACGGAACAACCTGTGCAAACGCATTGTCAGGAACCTGGGGAGAAAATATTGATGGCGCCACCTTTCAGGCTTATAAGTTTGACTTCTGTTGTAATATTTCTGGCGAAGTATACTCGAGTTTCTCTCTTTTGCTTGAGTCAACTCTCGCCGAGGATGTTGGTAAAGTTGAGATGGACCTTTACTTGGTCAGGAAGCTTGTCAAGGCTTCTGTCTCACCTTGTGGCCAGATACGTTTGAGTCAAGAGGAGCTGGTCAAAGCAAAATATTTTCAGCAGTTTTTCTTTAATGGCATGTTTGGAAAGTTGTTTGTTGGATCTAAGTCACAGGGAACAAAGAGAGAATTTTTGCTTCAAACTGACACTAGTTCTCTTTGGCACCCTGCCTTTATGTTTCTACTGCTACCAGTTGAAACAAATGATCTAGCTTCGAGTGCGACAATTGATTGGTCAGCTATCAACTCCTGTGCCTCAATAGTTGAGTTCTTGAAGAAAAATTCTCTTCTTGATCTTCGGGATAGTGATGGGAATCAGTGCAATACCTCATCCGGTCAGGAAGTCTTACTAGACGATAAAATGGAAGAAACGAATCTGATTCATTTTGCCAATGCTTCGTCTGATAAAAATAGTCTCGAAGAACTTGTGGTCATTGCAATTCATACTGGACGGATATACTCTATAGTTGAAGCCGTAAGCGATTCTTCTGCTATGAGCCCCTTTGAGGTGGATGCCTCATCAGGCTATGCTACTTATGCAGAATATTTTAACAAAAAGTATGGGATTGTTTTAGCGCACCCGAACCAGCCGTTGATGAAGTTGAAGCAGAGTCACCATGCGCACAACCTTTTAGTCGACTTCAATGAAGAGATGGTTGTGAAGACAGAACCAAAAGCTGGCAATGTTAGGAAAAGAAAACCGAATATCCATGCGCATTTGCCTCCAGAGCTTTTGGCTAGAATTGATGTACCGCGTGCTGTGCTAAAATCAATCTACTTGCTGCCTTCAGTGATGCACCGCCTAGAGTCTCTAATGTTGGCCAGCCAGCTTAGGGAAGAGATTGATTGTAGCATAGATAACTTCAGTATATCAAGTACATCGATTCTTGAAGCAGTTACAACACTTACATGCCCCGAATCATTTTCAATGGAGCGGTTGGAACTGCTCGGGGATTCAGTCTTGAAGTATGTTGCGAGCTGTCATCTATTCCTTAAGTATCCTGACAAAGATGAGGGGCAACTATCACGGCAGAGACAATCGATTATATCTAACTCAAATCTTCACCGCTTGACAACCAGTCGCAAACTACAGGGATACATAAGAAATGGCGCTTTTGAACCGCGTCGCTGGACTGCACCTGGTCAATTTTCTCTTTTTCCTGTTCCTTGCAAGTGTGGGATTGATACTAGAGAAGTACCATTGGACCCAAAATTCTTCACAGAAAACATGACTATCAAAATAGGCAAGTCTTGCGACATGGGTCATAGATGGGTAGTTTCAAAATCTGTATCAGATTGCGCTGAGGCCCTGATTGGTGCCTATTATGTAAGCGGTGGATTGTCTGCTTCTCTCCATATGATGAAATGGCTCGGTATTGACGTCGATTTTGACCCAAACCTAGTCGTTGAAGCCATCAATAGAGTTTCTCTACGGTGTTACATTCCTAAAGAAGATGAGCTCATAGAGTTGGAGAGAAAGATCCAACATGAATTCTCTGCAAAGTTTCTTTTAAAAGAGGCTATCACACACTCCTCTCTTCGTGAATCCTATTCATACGAGAGATTAGAGTTTCTTGGCGATTCTGTACTGGATTTTCTAATAACCCGTCATCTTTTTAACACCTACGAACAAACTGGGCCTGGAGAGATGACCGATCTTCGTTCTGCATGTGTAAACAATGAAAATTTTGCGCAAGTTGCAGTGAAAAATAACCTGCATACCCACCTTCAACGCTGTGCTACGGTTCTCGAGACTCAAATAAACGACTATCTGATGTCCTTTCAAAAGCCAGATGAGACTGGTAGATCAATCCCTTCAATACAGGGCCCTAAGGCTCTTGGAGATGTTGTGGAGAGTATCGCTGGAGCATTGCTGATCGATACGAGGTTAGATCTCGATCAAGTGTGGAGAGTCTTTGAGCCGTTGCTTTCTCCACTTGTAACTCCAGATAAACTTCAGCTTCCTCCATACCGGGAGCTCAATGAGCTATGCGACTCTCTTGGGTATTTCTTTCGAGTGAAATGTTCAAATGATGGTGTCAAAGCACAAGCCACGATCCAGTTGCAGCTGGATGATGTTCTTTTAACTGGAGATGGATCTGAACAGACAAATAAACTGGCCTTGGGAAAAGCAGCTTCACATCTGCTTACACAACTTGAGAAGAGAAACATTTCACGTAAAACCTCGCTCGGGGATAATCAAAGTTCCATGGATGTCAATCTTGCTTGCAATCATAGCGACAGAGAAACTCTGACTTCAGAGACTACTGAAATCCAGAGTATAGTGATTCCATTTATTGGACCTATAAACATGAAGAAAGGCGGGCCTCGTGGAACTCTACATGAGTTTTGCAAGAAGCATCTGTGGCCAATGCCTACTTTCGATACCTCGGAAGAGAAATCCAGAACTCCGTTTGAATTCATAGATGGCGGTGAGAAGCGGACTAGCTTCAGCAGTTTCACATCGACCATAACCCTAAGGATACCCAATCGTGAGGCTGTGATGTATGCTGGAGAAGCAAGGCCTGACAAGAAGAGTTCCTTCGACTCTGCAGTCGTGGAATTGCTTTATGAGCTCGAGCGCCGCAAGATCGTCATAATACAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009892 GO:0009987 GO:0010033 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0031974 GO:0031981 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0098586 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1531

Amino Acids

172.01

Weight (kDa)

5.9

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 53 - 181 9.7e-08 DEAD/DEAH box helicase
ResIII PF04851 55 - 187 8.9e-06 Type III restriction enzyme, res subunit
Helicase_C PF00271 346 - 458 7.2e-09 Helicase conserved C-terminal domain
PAZ PF02170 797 - 920 1.9e-11 PAZ domain
Ribonuclease_3 PF00636 973 - 1108 2.2e-18 Ribonuclease III domain
Ribonucleas_3_3 PF14622 973 - 1031 1.3e-07 Ribonuclease-III-like
Ribonucleas_3_3 PF14622 1164 - 1289 2.2e-12 Ribonuclease-III-like
Ribonuclease_3 PF00636 1182 - 1290 1.3e-20 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 891, 1115, 1848
AasI GACNNNNNNGTC 1 cut(s) 4538
AatII GACGTC 1 cut(s) 3369
Acc16I TGCGCA 4 cut(s) 826, 2607, 2700, 3678
Acc36I ACCTGC 3 cut(s) 1350, 1739, 3708
AccB1I GGYRCC 3 cut(s) 1829, 2148, 3302
AccB7I CCANNNNNTGG 3 cut(s) 1057, 2663, 4283
AccBSI CCGCTC 1 cut(s) 2920
AccI GTMKAC 2 cut(s) 1884, 2622
AccII CGCG 2 cut(s) 2740, 3109
AclWI GGATC 8 cut(s) 1481, 1551, 1564, 1619, 2095, 3460, 4036, 4090
AcoI YGGCCR 3 cut(s) 1996, 2805, 4352
AcuI CTGAAG 7 cut(s) 582, 861, 2757, 2833, 3926, 4227, 4420
AcyI GRCGYC 2 cut(s) 1830, 3366
AdeI CACNNNGTG 2 cut(s) 1543, 1994
AfaI GTAC 6 cut(s) 155, 347, 2736, 2863, 3183, 3573
AfeI AGCGCT 1 cut(s) 386
AfiI CCNNNNNNNGG 8 cut(s) 945, 1057, 1957, 2491, 2663, 2818, 4176, 4283
AflII CTTAAG 2 cut(s) 56, 2978
AhdI GACNNNNNGTC 1 cut(s) 3063
AhlI ACTAGT 1 cut(s) 2135
AjnI CCWGG 3 cut(s) 1808, 3124, 3625
AjuI GAANNNNNNNTTGG 2 cut(s) 333, 365
AleI CACNNNNGTG 1 cut(s) 1286
AloI GAACNNNNNNTCC 2 cut(s) 1293, 1325
Alw21I GWGCWC 4 cut(s) 322, 3447, 3966, 4563
AlwI GGATC 8 cut(s) 1481, 1551, 1564, 1619, 2095, 3460, 4036, 4090
Ama87I CYCGRG 5 cut(s) 1888, 2933, 3734, 4175, 4562
Aor51HI AGCGCT 1 cut(s) 386
ApaI GGGCCC 1 cut(s) 3815
ApeKI GCWGC 6 cut(s) 988, 1601, 1745, 2767, 4051, 4118
ArsI GACNNNNNNTTYG 4 cut(s) 1784, 1816, 3356, 3388
Asp700I GAANNNNTTC 2 cut(s) 49, 4156
AsuC2I CCSGG 1 cut(s) 3959
AsuHPI GGTGA 7 cut(s) 621, 1085, 1276, 1980, 2591, 3041, 4430
AvaI CYCGRG 5 cut(s) 1888, 2933, 3734, 4175, 4562
AvaII GGWCC 4 cut(s) 608, 1948, 3190, 4286
AxyI CCTNAGG 2 cut(s) 3816, 4463
BaeGI GKGCMC 1 cut(s) 3815
BaeI ACNNNNGTAYC 2 cut(s) 1194, 1227
BalI TGGCCA 3 cut(s) 1998, 2807, 4354
BamHI GGATCC 1 cut(s) 1556
BanI GGYRCC 3 cut(s) 1829, 2148, 3302
BanII GRGCYC 5 cut(s) 2486, 3447, 3815, 3966, 4563
BarI GAAGNNNNNNTAC 2 cut(s) 1720, 1752
BauI CACGAG 1 cut(s) 4314
BbsI GAAGAC 2 cut(s) 479, 2654
Bbv12I GWGCWC 4 cut(s) 322, 3447, 3966, 4563
BbvCI CCTCAGC 1 cut(s) 3288
BbvI GCAGC 6 cut(s) 1000, 1613, 1732, 2754, 4063, 4130
BceAI ACGGC 5 cut(s) 1714, 2444, 2560, 3029, 3892
BcgI CGANNNNNNTGC 4 cut(s) 2456, 2490, 4030, 4064
BciT130I CCWGG 3 cut(s) 1810, 3126, 3627
BciVI GTATCC 3 cut(s) 2994, 3074, 4461
BcnI CCSGG 1 cut(s) 3959
BcuI ACTAGT 1 cut(s) 2135
BfmI CTRYAG 6 cut(s) 637, 781, 1746, 2448, 3074, 4536
BfoI RGCGCY 4 cut(s) 388, 1833, 3099, 4570
BfrI CTTAAG 2 cut(s) 56, 2978
BfuAI ACCTGC 3 cut(s) 1350, 1739, 3708
BfuI GTATCC 3 cut(s) 2994, 3074, 4461
BglI GCCNNNNNGGC 1 cut(s) 1160
BglII AGATCT 1 cut(s) 3877
BisI GCNGC 9 cut(s) 837, 959, 989, 1602, 1746, 2768, 4052, 4119, 4570
BlsI GCNGC 9 cut(s) 838, 960, 990, 1603, 1747, 2769, 4053, 4120, 4571
BmcAI AGTACT 1 cut(s) 347
Bme1390I CCNGG 4 cut(s) 1810, 3126, 3627, 3959
Bme18I GGWCC 4 cut(s) 608, 1948, 3190, 4286
BmeRI GACNNNNNGTC 1 cut(s) 3063
BmeT110I CYCGRG 5 cut(s) 1888, 2933, 3734, 4175, 4562
BmrFI CCNGG 4 cut(s) 1810, 3126, 3627, 3959
BmrI ACTGGG 1 cut(s) 3630
BmsI GCATC 4 cut(s) 676, 2488, 2769, 4354
BmuI ACTGGG 1 cut(s) 3630
BoxI GACNNNNGTC 1 cut(s) 3246
BpiI GAAGAC 2 cut(s) 479, 2654
BplI GAGNNNNNCTC 6 cut(s) 845, 877, 1612, 1644, 1882, 1914
BpmI CTGGAG 7 cut(s) 1506, 2694, 3648, 3870, 3915, 4095, 4518
Bpu10I CCTNAGC 2 cut(s) 2816, 3288
BpuEI CTTGAG 4 cut(s) 662, 944, 1928, 4163
BpuMI CCSGG 1 cut(s) 3959
Bsa29I ATCGAT 4 cut(s) 78, 2867, 3026, 3864
BsaAI YACGTR 1 cut(s) 4163
BsaBI GATNNNNATC 6 cut(s) 84, 90, 1680, 2298, 3882, 4203
BsaHI GRCGYC 2 cut(s) 1830, 3366
BsaI GGTCTC 1 cut(s) 1437
BsaJI CCNNGG 6 cut(s) 1560, 1809, 1923, 4107, 4194, 4374
BsaWI WCCGGW 1 cut(s) 2318
BsaXI ACNNNNNCTCC 4 cut(s) 1184, 1214, 3819, 3849
Bsc4I CCNNNNNNNGG 8 cut(s) 945, 1057, 1957, 2491, 2663, 2818, 4176, 4283
Bse21I CCTNAGG 2 cut(s) 3816, 4463
Bse3DI GCAATG 4 cut(s) 796, 2418, 2674, 3854
Bse8I GATNNNNATC 6 cut(s) 84, 90, 1680, 2298, 3882, 4203
BseBI CCWGG 3 cut(s) 1810, 3126, 3627
BseCI ATCGAT 4 cut(s) 78, 2867, 3026, 3864
BseDI CCNNGG 6 cut(s) 1560, 1809, 1923, 4107, 4194, 4374
BseGI GGATG 9 cut(s) 397, 451, 526, 632, 1933, 2315, 2503, 4063, 4204
BseJI GATNNNNATC 6 cut(s) 84, 90, 1680, 2298, 3882, 4203
BseLI CCNNNNNNNGG 8 cut(s) 945, 1057, 1957, 2491, 2663, 2818, 4176, 4283
BseMI GCAATG 4 cut(s) 796, 2418, 2674, 3854
BseMII CTCAG 4 cut(s) 395, 876, 1584, 3279
BseRI GAGGAG 3 cut(s) 420, 2033, 3508
BseSI GKGCMC 1 cut(s) 3815
BseXI GCAGC 6 cut(s) 1000, 1613, 1732, 2754, 4063, 4130
BseYI CCCAGC 1 cut(s) 387
BsgI GTGCAG 1 cut(s) 3105
Bsh1236I CGCG 2 cut(s) 2740, 3109
BshNI GGYRCC 3 cut(s) 1829, 2148, 3302
BshVI ATCGAT 4 cut(s) 78, 2867, 3026, 3864
BsiHKAI GWGCWC 4 cut(s) 322, 3447, 3966, 4563
BsiHKCI CYCGRG 5 cut(s) 1888, 2933, 3734, 4175, 4562
BsiSI CCGG 3 cut(s) 20, 2319, 3958
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 8 cut(s) 945, 1057, 1957, 2491, 2663, 2818, 4176, 4283
BsmFI GGGAC 1 cut(s) 208
BsmI GAATGC 4 cut(s) 4, 461, 509, 902
Bso31I GGTCTC 1 cut(s) 1437
BsoBI CYCGRG 5 cut(s) 1888, 2933, 3734, 4175, 4562
Bsp120I GGGCCC 1 cut(s) 3811
Bsp1286I GDGCHC 6 cut(s) 322, 2486, 3447, 3815, 3966, 4563
Bsp19I CCATGG 2 cut(s) 1560, 4194
BspCNI CTCAG 4 cut(s) 394, 875, 1585, 3280
BspDI ATCGAT 4 cut(s) 78, 2867, 3026, 3864
BspFNI CGCG 2 cut(s) 2740, 3109
BspMAI CTGCAG 2 cut(s) 1750, 4540
BspMI ACCTGC 3 cut(s) 1350, 1739, 3708
BspPI GGATC 8 cut(s) 1481, 1551, 1564, 1619, 2095, 3460, 4036, 4090
BspQI GCTCTTC 1 cut(s) 558
BspT107I GGYRCC 3 cut(s) 1829, 2148, 3302
BspTI CTTAAG 2 cut(s) 56, 2978
BspTNI GGTCTC 1 cut(s) 1437
BsrBI CCGCTC 1 cut(s) 2920
BsrDI GCAATG 4 cut(s) 796, 2418, 2674, 3854
BssECI CCNNGG 6 cut(s) 1560, 1809, 1923, 4107, 4194, 4374
BssNAI GTATAC 1 cut(s) 1885
BssNI GRCGYC 2 cut(s) 1830, 3366
BssSI CACGAG 1 cut(s) 4314
BssT1I CCWWGG 3 cut(s) 1560, 4107, 4194
Bst1107I GTATAC 1 cut(s) 1885
Bst2BI CACGAG 1 cut(s) 4314
Bst2UI CCWGG 3 cut(s) 1810, 3126, 3627
Bst4CI ACNGT 8 cut(s) 292, 598, 725, 1541, 1588, 1656, 3420, 3730
BstACI GRCGYC 2 cut(s) 1830, 3366
BstAFI CTTAAG 2 cut(s) 56, 2978
BstAPI GCANNNNNTGC 1 cut(s) 3237
BstBAI YACGTR 1 cut(s) 4163
BstC8I GCNNGC 8 cut(s) 407, 2665, 2742, 2809, 2813, 2962, 4213, 4310
BstDSI CCRYGG 2 cut(s) 1560, 4194
BstF5I GGATG 9 cut(s) 397, 451, 526, 632, 1933, 2315, 2503, 4063, 4204
BstFNI CGCG 2 cut(s) 2740, 3109
BstH2I RGCGCY 4 cut(s) 388, 1833, 3099, 4570
BstNI CCWGG 3 cut(s) 1810, 3126, 3627
BstNSI RCATGY 5 cut(s) 1025, 1385, 2068, 2898, 3657
BstPAI GACNNNNGTC 1 cut(s) 3246
BstSCI CCNGG 4 cut(s) 1808, 3124, 3625, 3957
BstSFI CTRYAG 6 cut(s) 637, 781, 1746, 2448, 3074, 4536
BstSLI GKGCMC 1 cut(s) 3815
BstUI CGCG 2 cut(s) 2740, 3109
BstV1I GCAGC 6 cut(s) 1000, 1613, 1732, 2754, 4063, 4130
BstV2I GAAGAC 2 cut(s) 479, 2654
BstX2I RGATCY 6 cut(s) 1486, 1556, 2087, 3465, 3877, 4082
BstXI CCANNNNNNTGG 1 cut(s) 518
BstYI RGATCY 6 cut(s) 1486, 1556, 2087, 3465, 3877, 4082
BstZ17I GTATAC 1 cut(s) 1885
Bsu15I ATCGAT 4 cut(s) 78, 2867, 3026, 3864
Bsu36I CCTNAGG 2 cut(s) 3816, 4463
BsuI GTATCC 3 cut(s) 2994, 3074, 4461
BsuTUI ATCGAT 4 cut(s) 78, 2867, 3026, 3864
BtgI CCRYGG 2 cut(s) 1560, 4194
BtsCI GGATG 9 cut(s) 397, 451, 526, 632, 1933, 2315, 2503, 4063, 4204
BtsI GCAGTG 2 cut(s) 1094, 3693
BtsIMutI CAGTG 6 cut(s) 516, 1094, 1530, 2309, 2781, 3693
BveI ACCTGC 3 cut(s) 1350, 1739, 3708
Cac8I GCNNGC 8 cut(s) 407, 2665, 2742, 2809, 2813, 2962, 4213, 4310
ClaI ATCGAT 4 cut(s) 78, 2867, 3026, 3864
CseI GACGC 1 cut(s) 3098
CsiI ACCWGGT 1 cut(s) 3124
Csp6I GTAC 6 cut(s) 154, 346, 2735, 2862, 3182, 3572
CviQI GTAC 6 cut(s) 154, 346, 2735, 2862, 3182, 3572
DinI GGCGCC 1 cut(s) 1831
DraI TTTAAA 1 cut(s) 3498
DraIII CACNNNGTG 2 cut(s) 1543, 1994
DrdI GACNNNNNNGTC 1 cut(s) 4538
DriI GACNNNNNGTC 1 cut(s) 3063
DseDI GACNNNNNNGTC 1 cut(s) 4538
EaeI YGGCCR 3 cut(s) 1996, 2805, 4352
Eam1105I GACNNNNNGTC 1 cut(s) 3063
Ecl136II GAGCTC 3 cut(s) 3445, 3964, 4561
Eco130I CCWWGG 3 cut(s) 1560, 4107, 4194
Eco147I AGGCCT 1 cut(s) 4510
Eco24I GRGCYC 5 cut(s) 2486, 3447, 3815, 3966, 4563
Eco31I GGTCTC 1 cut(s) 1437
Eco47I GGWCC 4 cut(s) 608, 1948, 3190, 4286
Eco47III AGCGCT 1 cut(s) 386
Eco53kI GAGCTC 3 cut(s) 3445, 3964, 4561
Eco57I CTGAAG 7 cut(s) 582, 861, 2757, 2833, 3926, 4227, 4420
Eco81I CCTNAGG 2 cut(s) 3816, 4463
Eco88I CYCGRG 5 cut(s) 1888, 2933, 3734, 4175, 4562
EcoICRI GAGCTC 3 cut(s) 3445, 3964, 4561
EcoO109I RGGNCCY 3 cut(s) 3292, 3811, 3812
EcoRI GAATTC 2 cut(s) 3476, 4403
EcoRII CCWGG 3 cut(s) 1808, 3124, 3625
EcoT14I CCWWGG 3 cut(s) 1560, 4107, 4194
EcoT22I ATGCAT 2 cut(s) 6, 1328
EcoT38I GRGCYC 5 cut(s) 2486, 3447, 3815, 3966, 4563
EgeI GGCGCC 1 cut(s) 1831
EheI GGCGCC 1 cut(s) 1831
ErhI CCWWGG 3 cut(s) 1560, 4107, 4194
FaqI GGGAC 1 cut(s) 208
FauI CCCGC 1 cut(s) 4301
FauNDI CATATG 1 cut(s) 3342
FblI GTMKAC 2 cut(s) 1884, 2622
Fnu4HI GCNGC 9 cut(s) 837, 959, 989, 1602, 1746, 2768, 4052, 4119, 4570
FokI GGATG 9 cut(s) 404, 458, 513, 619, 1940, 2302, 2510, 4070, 4211
FriOI GRGCYC 5 cut(s) 2486, 3447, 3815, 3966, 4563
Fsp4HI GCNGC 9 cut(s) 837, 959, 989, 1602, 1746, 2768, 4052, 4119, 4570
FspAI RTGCGCAY 2 cut(s) 2607, 2700
FspI TGCGCA 4 cut(s) 826, 2607, 2700, 3678
GluI GCNGC 9 cut(s) 837, 959, 989, 1602, 1746, 2768, 4052, 4119, 4570
GsaI CCCAGC 1 cut(s) 391
GsuI CTGGAG 7 cut(s) 1506, 2694, 3648, 3870, 3915, 4095, 4518
HaeII RGCGCY 4 cut(s) 388, 1833, 3099, 4570
HapII CCGG 3 cut(s) 20, 2319, 3958
HgaI GACGC 1 cut(s) 3098
Hin1I GRCGYC 2 cut(s) 1830, 3366
HincII GTYRAC 2 cut(s) 1914, 2623
HindII GTYRAC 2 cut(s) 1914, 2623
HindIII AAGCTT 1 cut(s) 1967
HpaII CCGG 3 cut(s) 20, 2319, 3958
HphI GGTGA 7 cut(s) 621, 1085, 1276, 1980, 2591, 3041, 4430
Hpy166II GTNNAC 6 cut(s) 154, 1517, 1885, 1914, 2623, 3661
Hpy8I GTNNAC 6 cut(s) 154, 1517, 1885, 1914, 2623, 3661
Hpy99I CGWCG 3 cut(s) 13, 3114, 3371
HpyCH4III ACNGT 8 cut(s) 292, 598, 725, 1541, 1588, 1656, 3420, 3730
HpyCH4IV ACGT 4 cut(s) 1762, 2005, 3366, 4162
HpySE526I ACGT 4 cut(s) 1762, 2005, 3366, 4162
Hsp92I GRCGYC 2 cut(s) 1830, 3366
KasI GGCGCC 1 cut(s) 1829
LguI GCTCTTC 1 cut(s) 558
LmnI GCTCC 7 cut(s) 16, 353, 1501, 2020, 2917, 3851, 3961
Lsp1109I GCAGC 6 cut(s) 1000, 1613, 1732, 2754, 4063, 4130
LweI GCATC 4 cut(s) 676, 2488, 2769, 4354
MabI ACCWGGT 1 cut(s) 3124
MaeII ACGT 4 cut(s) 1762, 2005, 3366, 4162
MaeIII GTNAC 9 cut(s) 1073, 1361, 1647, 1665, 2094, 2597, 2881, 3422, 3925
MbiI CCGCTC 1 cut(s) 2920
MfeI CAATTG 1 cut(s) 2211
MflI RGATCY 6 cut(s) 1486, 1556, 2087, 3465, 3877, 4082
MhlI GDGCHC 6 cut(s) 322, 2486, 3447, 3815, 3966, 4563
MlsI TGGCCA 3 cut(s) 1998, 2807, 4354
MluNI TGGCCA 3 cut(s) 1998, 2807, 4354
Mly113I GGCGCC 1 cut(s) 1830
MmeI TCCRAC 5 cut(s) 1719, 2065, 2904, 3435, 3493
Mox20I TGGCCA 3 cut(s) 1998, 2807, 4354
Mph1103I ATGCAT 2 cut(s) 6, 1328
MroXI GAANNNNTTC 2 cut(s) 49, 4156
MscI TGGCCA 3 cut(s) 1998, 2807, 4354
MslI CAYNNNNRTG 5 cut(s) 531, 947, 1286, 1452, 2893
Msp20I TGGCCA 3 cut(s) 1998, 2807, 4354
MspA1I CMGCKG 1 cut(s) 4054
MspCI CTTAAG 2 cut(s) 56, 2978
MspI CCGG 3 cut(s) 20, 2319, 3958
MspR9I CCNGG 4 cut(s) 1810, 3126, 3627, 3959
MunI CAATTG 1 cut(s) 2211
Mva1269I GAATGC 4 cut(s) 4, 461, 509, 902
MvaI CCWGG 3 cut(s) 1810, 3126, 3627
MvnI CGCG 2 cut(s) 2740, 3109
NarI GGCGCC 1 cut(s) 1830
NciI CCSGG 1 cut(s) 3959
NcoI CCATGG 2 cut(s) 1560, 4194
NdeI CATATG 1 cut(s) 3342
NmeAIII GCCGAG 1 cut(s) 1948
NmuCI GTSAC 3 cut(s) 1073, 2094, 2597
NsbI TGCGCA 4 cut(s) 826, 2607, 2700, 3678
NsiI ATGCAT 2 cut(s) 6, 1328
NspI RCATGY 5 cut(s) 1025, 1385, 2068, 2898, 3657
OliI CACNNNNGTG 1 cut(s) 1286
PaeR7I CTCGAG 3 cut(s) 1888, 3734, 4562
PceI AGGCCT 1 cut(s) 4510
PciSI GCTCTTC 1 cut(s) 558
PcsI WCGNNNNNNNCGW 2 cut(s) 1137, 3363
PctI GAATGC 4 cut(s) 4, 461, 509, 902
PdmI GAANNNNTTC 2 cut(s) 49, 4156
PflMI CCANNNNNTGG 3 cut(s) 1057, 2663, 4283
PkrI GCNGC 9 cut(s) 838, 960, 990, 1603, 1747, 2769, 4053, 4120, 4571
PluTI GGCGCC 1 cut(s) 1833
Ppu21I YACGTR 1 cut(s) 4163
PshAI GACNNNNGTC 1 cut(s) 3246
PsiI TTATAA 3 cut(s) 891, 1115, 1848
Psp124BI GAGCTC 3 cut(s) 3447, 3966, 4563
Psp6I CCWGG 3 cut(s) 1808, 3124, 3625
PspFI CCCAGC 1 cut(s) 387
PspGI CCWGG 3 cut(s) 1808, 3124, 3625
PspOMI GGGCCC 1 cut(s) 3811
PspXI VCTCGAGB 2 cut(s) 1888, 4562
PstI CTGCAG 2 cut(s) 1750, 4540
PsuI RGATCY 6 cut(s) 1486, 1556, 2087, 3465, 3877, 4082
PvuII CAGCTG 1 cut(s) 4054
RsaI GTAC 6 cut(s) 155, 347, 2736, 2863, 3183, 3573
RsaNI GTAC 6 cut(s) 154, 346, 2735, 2862, 3182, 3572
RseI CAYNNNNRTG 5 cut(s) 531, 947, 1286, 1452, 2893
SacI GAGCTC 3 cut(s) 3447, 3966, 4563
SalI GTCGAC 1 cut(s) 2621
SapI GCTCTTC 1 cut(s) 558
SatI GCNGC 9 cut(s) 837, 959, 989, 1602, 1746, 2768, 4052, 4119, 4570
ScaI AGTACT 1 cut(s) 347
ScrFI CCNGG 4 cut(s) 1810, 3126, 3627, 3959
SduI GDGCHC 6 cut(s) 322, 2486, 3447, 3815, 3966, 4563
SexAI ACCWGGT 1 cut(s) 3124
SfaNI GCATC 4 cut(s) 676, 2488, 2769, 4354
SfcI CTRYAG 6 cut(s) 637, 781, 1746, 2448, 3074, 4536
SfoI GGCGCC 1 cut(s) 1831
Sfr274I CTCGAG 3 cut(s) 1888, 3734, 4562
SinI GGWCC 4 cut(s) 608, 1948, 3190, 4286
SlaI CTCGAG 3 cut(s) 1888, 3734, 4562
SmiMI CAYNNNNRTG 5 cut(s) 531, 947, 1286, 1452, 2893
SmlI CTYRAG 9 cut(s) 56, 677, 923, 1888, 1907, 2978, 3734, 4142, 4562
SmoI CTYRAG 9 cut(s) 56, 677, 923, 1888, 1907, 2978, 3734, 4142, 4562
SpeI ACTAGT 1 cut(s) 2135
SseBI AGGCCT 1 cut(s) 4510
SspDI GGCGCC 1 cut(s) 1829
SspI AATATT 6 cut(s) 438, 615, 1822, 1870, 2039, 2531
SstI GAGCTC 3 cut(s) 3447, 3966, 4563
StuI AGGCCT 1 cut(s) 4510
StyD4I CCNGG 4 cut(s) 1808, 3124, 3625, 3957
StyI CCWWGG 3 cut(s) 1560, 4107, 4194
TaaI ACNGT 8 cut(s) 292, 598, 725, 1541, 1588, 1656, 3420, 3730
TaiI ACGT 4 cut(s) 1765, 2008, 3369, 4165
TaqII GACCGA 1 cut(s) 3653
TatI WGTACW 3 cut(s) 345, 2861, 3571
TauI GCSGC 3 cut(s) 839, 961, 4572
TscAI CASTG 6 cut(s) 523, 1094, 1530, 2309, 2781, 3693
TseFI GTSAC 3 cut(s) 1073, 2094, 2597
TseI GCWGC 6 cut(s) 988, 1601, 1745, 2767, 4051, 4118
Tsp45I GTSAC 3 cut(s) 1073, 2094, 2597
TspGWI ACGGA 3 cut(s) 1791, 2452, 4386
TspRI CASTG 6 cut(s) 523, 1094, 1530, 2309, 2781, 3693
Van91I CCANNNNNTGG 3 cut(s) 1057, 2663, 4283
Vha464I CTTAAG 2 cut(s) 56, 2978
VpaK11BI GGWCC 4 cut(s) 608, 1948, 3190, 4286
XceI RCATGY 5 cut(s) 1025, 1385, 2068, 2898, 3657
XcmI CCANNNNNNNNNTGG 1 cut(s) 515
XhoI CTCGAG 3 cut(s) 1888, 3734, 4562
XmiI GTMKAC 2 cut(s) 1884, 2622
XmnI GAANNNNTTC 2 cut(s) 49, 4156
ZraI GACGTC 1 cut(s) 3367
ZrmI AGTACT 1 cut(s) 347
Zsp2I ATGCAT 2 cut(s) 6, 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.