Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0001909

Physical Location & Seq

Genomic Coordinates Forward (+)

50093231 .. 50104563

Gene Structure 11333 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG279000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4410 bp

ATGAGTCTCACTGCTCTTGACATTTCCCTTGAAGGATCACAATCTCCCCATGATATCAAGCATTTCGTCCCCAGAAGGTATCAAGTGAAAGTGTTCGAATTCGCAAAGCGTAAGAACATAATTGCAGTGTTGGAAACTGGTGCTGGGAAAACTATGATTGCTGTGATGCTCATCAAACACATTGCTGATCAACACATCATCAAGTCCACTGGCCACAAGAAGTTGATAATTTTCATGGCCCCAACAGTTCATCTTGTCATTCAGCAATATGAGGTTATAAAAAAGTCTACAACTCTTGAAGTGGGAGTATACTACGGAGCTAAGGGCGTTGATGACTGGAGTCCAGAATATTGGGAGGTGGAAGTGAATACACATAATATAATGGTCATGACACCTCAAATTCTCCTGGATGCTTTGCGGAATGCATTTTTGAAGATAGAGATGATTTGCTTGATGGTCTTTGATGAATGCCACCGTGCCACTGGCAATCATCCCTACACGAAAATAATGAAGGAATTTCATCACAAATCAGGAAACAAGCCAAAGATTTTTGGAATGACAGCATCACCAGTCATTAAAAAAGGTGTCTCATCCGCCGCGGACTGTGAGGATCAGATATCACAACTTGAAAGCATCTTGGATTCCCAGGTTTATACAGTAGAAGACAGGACAGAGATTGAAGAGCATGTTCCCTCAGCAGTCCAAATTTGTAAATTTTATGACCCGACATGGTTGATCTGTGTGGATTTGAAAGCAAAAATGGAAGCTTTATGGTCAAAGTTTGATGCTTCTATCATGTCAAAGTTGCAAGGGCCAGAGAAAAGCGAGTACGAAGACATTAATGATAAAATCAAGATGCTACGAAAGCGGTTGTCCAATGACTACATGAAAATTTTGTATTGCCTTGATGATCTTGGTATCGTATGCGCATATGAGGCTGTGAAAGTCTGTCTGGAGAATGCCCCTGATGTCAAAGAGGAGTGCACATTTTTTAGAGAAAGCTCTTTGCAATGCAGATATTTTCTTGATGAAGTCTTAAATATGATCAGTGAATTTCTTCAAAAAGATTATATACTTCATACAGATCTTGAGTCAGACTATATAAAGGCACATAAAGTGGGATACATATCTCCGAAGTTACATGAACTTCTTCAAATTTTCCACTCATTTGAGAGGGATAGGCAAGTTCTGTGCCTTATTTTCGTAGAGAGAATTGTCACAGCCAAAGTAATGCAAAGATATGTGAAGAAACTGCCATGTTTATCTCATTTCACAGCTTCATATTTGACCGGAAGTACCACATCAGTTGATGCACTTGCACCGAAATTGCAGAAGGAAATTTTGGAGTCATTTTACAGTGGGAAGGTCAATCTACTATTTGCCACTGATGTAGTTGAGGAGGGAATTCATGTCCAGAACTGCTCCTGTGTGATCCGGTTTGATCTTCCCAAGACAGTTCGTAGCTATGTTCAGTCTCGGGGACGAGCACGAAAAAATGATTCCCAATATATCATAATGCTTGAAAGGGACAACAAGAAGCAAAGAGACCATCTCTTTGACATAATCAGAAGTGAGTACTCAATGACAGATACATCGAGAAATCGAGATCCTGATGGATGCAATTTAAGAGCTTGTAACTTTGAAGATGCAAATGCATACTATGTAGAAGCTACTGGAGCATCAGTTACTCCAGACTCTAGTGTCAGCCTCATATATCGGTACTGTGAAAAGCTCCCTGGAGATAGGTACTTTATTCCTAGGCCAAATTTTCACTTCAGACTATTTGAAGGGTTGTATCAGTGTGAAATAACATTACCTCCAAATGCGGCATTTCAAACACTAGTTGGTCCTATGAGTAGAAACGGTCATTCATCAAAGCAGCTTGTATGCTTGGAAGCTTGTAAAATGCTGCACCAAATGGGTGCTTTGGATGATCATCTTCTCCCACTTGTCGAAGTGCCTCTTGATAATGAAGTGATTGTGAAAAGCAAAGAATTATCTGCAGGTGCTGGAACTACAAAAAGGAAAGAGTTACATGGGATGACTTGCATTCGTGCATTAAGTGGAACTTGGAGAGAGAAAGTTGATGGTGCCAGTTTTGAGGCCTACAAATTTGAGTTCTCCTGTAATGTTGATGAGACATATTCAGGATTTGTACTACTAGTCGAGATGAAACTTGATGATGACGTGGGAAATACTGAAGTGGATCTCTACTTAATTGCAAAGATGGTCAAAGCTTACGTTTCTTCATGTGGAAAAGTGCATCTCAGTGCAGATCAGATAAAGGAAGCCATGTGCTTTCAGGAATTTTTCTTTAATGGTTTGTTTGGGAGATTGTTTCTTGGATCCGAAGCAAAAGGCACAGAAAGAAAATTTCTACTTCAGACTGAAATAAAATCACTATGGAGCTCATCGTACAGCTATTTACTTTTACCGACTGAGGCATCTGACAGTTCAAATAAGAAATCTTGGAACATAGATTGGATGGGAATCTGTTCCTGTGCTAATGTGGTGGAGTTTCTTAGGAAACACTATTCCAATGGTCTTGAGAATTTCAAAGGCGGTAGAGGAAATATATTACTTTCTAGGACTGGTTCATCCATGACAGATTGCAAGGCTGAAAATTTAGTCCAGTTTGCTAATAGTTCAATTGACGTCAATGATCTTGAAGATATGGTCGTATTGGCTATTCACACAGGAAGAATCTACTCTGTTATTGAAGTTGTTAGTAGCATTTCTGCTGAGAGTCCCTTGGAAGAAAATGCTGATGCTGCCTCATCAGATTATACTTATGTCGAGTACTTCAAAACAACGTATGGGATTGTACTCAAGTATCCTGGACATCCTCTATTGCGGTTGAAGCAAAGTCATAATGCACATAACCTGCTTGTTGATCAAGGTGCGTCATCACCAGATGGTCTAGTTCAGGAAAAGCAACAAGCTCATGTTCATATGCCACCTGAAATCTTAGTCAGCATTGATGTTCGAGTTGACGTTCTAAAATCATTTTACTTACTACCATCACTAATGTATCGTCTGGAGTCCCTAATGTTAGCCAGCCAGATTAGGAAAGAGATCAATGGCCAGTCTAGCAACTTTTATATATCAAGCTCATTGATTCTAGAAGCACTCACAACACTTAGATGCTGTGAAAATTTTTCATTGGAGCGTTTGGAGTTGCTTGGAGATTCAGTTCTCAAATATGCTGTGAGTTCCTACCTCTTTCTTAAATATCCAGAGAAACATGAAGGACAATTAACTGCTCGACGAACAAGAGCTATTTGCAACTCAAACCTGCATAAACTAGGAACGAAAAACAAGTTACAGGGATACATACGAGACAGTGCATTTGAACCACGACGTTGGGCTGCTCCAGGGCAGCTTTCTAGATTCCCGGATCCATGTCAATGTGGAGTGGATACTTTGGAAGTGCCTTTGGACAGTAGATTTCAAACTGAAAAGGCTGTCAAGGTTGGAAAATTCTGTGATAAAGGTCACAGATGGATGAACTCAAAAACCATTGCCGATTGTGTTGAGGCCCTCATAGGAGCTTATCATGTTGGTGGAGGATTGTTTGCTGCACTTCATTTGATGAAGTGGCTCAGCATTGATTCTGAAACCGGACCCTCATTAGCTGATGAAGCCATTACAAGGGCATCACTTCGATGCTACAATCCCAAAACTAATGAACTTGCTAACTTGGAGTTGAAGCTTAGCTATGAATTTTCAGTCAAGGGCTTGTTACATGAGGCCATCACACATGCTTCTCAGCAAAAACTAGGTGCCAGCTACTGTTATCAGAGGCTTGAATTTCTTGGAGACTCTGTGTTGGATGTGCTAACTACACAGCACCTCTATCGCAGCCACACAGATATTGATCCTGGAGTGTTGACTGATTTGCGGTCAGCTGCTGTTAGTAATGAAAAGTTTGCTCAAGCTGCTATTAGACAAAACCTTCATCCACATCTTCAACATTGCTCTGGGTTACTTCGGAGTCACATAACCGAGTATGAGAGGTTGCTTACAGAAGCTGAGAACAGTACCAGCTCACTGGAAGACATTAAGGGTCCAAAGGCTCTTGGTGACATGGTGGAAAGTATAGCAGGAGCTATACTAATTGATACAAAGCTTAATCTTGATGAAGTATGGAGGGTTTTTAAACCAATATTGTCTCCAATTGTGACCCCTTCTAGTCTTCAATTGCATCCGCTACGTAAACTGTCTGAATTATGTGATTCACTTGGGTACTTTGTGAAAGAAACATGTACTAAAGATGATACACTAGTGCATGTGGAACTAAGTTTGCAGCTGGAAGATGTTCTTTTGGTTGGAAATGGCTTTGATCGGAGTAGAAAAGCTGCGAAACATAAGGCAGCTCGTCAAGTGTTGAAGGCGCTCGAGAGCCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1469

Amino Acids

165.93

Weight (kDa)

6.31

Isoelectric Point (pI)

40.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	26 - 190	4.3e-18	DEAD/DEAH box helicase
ResIII	PF04851	26 - 190	2.4e-16	Type III restriction enzyme, res subunit
Helicase_C	PF00271	379 - 496	2.4e-17	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	567 - 651	3.4e-23	Dicer dimerisation domain
PAZ	PF02170	886 - 1006	1.5e-09	PAZ domain
Ribonuclease_3	PF00636	1056 - 1187	2e-20	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1056 - 1113	2.5e-07	Ribonuclease-III-like
Ribonucleas_3_3	PF14622	1246 - 1374	1.9e-13	Ribonuclease-III-like
Ribonuclease_3	PF00636	1267 - 1375	1.4e-19	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001909)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920
fragaria_vesca	FvH4_2g07790 FvH4_2g18930 FvH4_2g18930 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780
malus_domestica	MD08G1046300.v1.1 MD10G1163700.v1.1 MD15G1047500.v1.1
prunus_persica	Prupe.1G401900_v2.0.a1 Prupe.1G401900_v2.0.a1 Prupe.8G202000_v2.0.a1 Prupe.8G202000_v2.0.a1
pyrus_communis	pycom08g03830 pycom08g03840 pycom15g04670
rosa_chinensis	RchiOBHm_Chr6g0284721 RchiOBHm_Chr6g0312121
rosa_laevigata	RLG00000010353 RLG00000012724
rosa_multiflora	Rmu_sc0004065.1_g000007 Rmu_sc0007474.1_g000021
rosa_roxburghii	Rroxscaffold_7G00157050 Rroxscaffold_7G00184440
rosa_rugosa	Rorug06G0165900 Rorug06G0392600 Rorug06G0392700 Rorug06G0392800
rosa_samantha	Rh4CG118000 Rh6AG279000 Rh6AG509700 Rh6BG279000 Rh6BG520600 Rh6CG281300 Rh6CG526600 Rh6DG274000 Rh6DG512000
rosa_wichuraiana	Rw6G023980 Rw6G044350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	278
AarI	CACCTGC	1 cut(s)	1994
AasI	GACNNNNNNGTC	1 cut(s)	1700
AatII	GACGTC	1 cut(s)	2658
Acc16I	TGCGCA	1 cut(s)	928
Acc36I	ACCTGC	3 cut(s)	1994, 2892, 3303
AccB1I	GGYRCC	2 cut(s)	2090, 3782
AccI	GTMKAC	2 cut(s)	287, 309
AccII	CGCG	1 cut(s)	599
AcoI	YGGCCR	2 cut(s)	211, 3082
AcuI	CTGAAG	3 cut(s)	1759, 2220, 2366
AcyI	GRCGYC	1 cut(s)	2655
AfiI	CCNNNNNNNGG	1 cut(s)	3651
AflIII	ACRYGT	1 cut(s)	4262
AhlI	ACTAGT	3 cut(s)	1840, 2161, 4282
AjiI	CACGTC	1 cut(s)	2188
AjnI	CCWGG	6 cut(s)	405, 645, 1735, 2836, 3373, 3880
AjuI	GAANNNNNNNTTGG	2 cut(s)	1325, 1357
AloI	GAACNNNNNNTCC	2 cut(s)	3177, 3209
Alw21I	GWGCWC	3 cut(s)	986, 1489, 2411
Alw26I	GTCTC	8 cut(s)	11, 592, 1479, 1539, 2132, 3333, 3813, 4176
Alw44I	GTGCAC	1 cut(s)	982
AlwNI	CAGNNNCTG	4 cut(s)	2009, 3792, 3911, 4031
Ama87I	CYCGRG	2 cut(s)	1476, 4397
AoxI	GGCC	8 cut(s)	211, 237, 812, 1760, 2103, 3082, 3537, 3750
ApaLI	GTGCAC	1 cut(s)	982
ArsI	GACNNNNNNTTYG	4 cut(s)	1538, 1570, 3332, 3364
AseI	ATTAAT	1 cut(s)	840
Asp700I	GAANNNNTTC	5 cut(s)	92, 1056, 1149, 2494, 4317
AspA2I	CCTAGG	1 cut(s)	1757
AspLEI	GCGC	2 cut(s)	929, 4396
AspS9I	GGNCC	6 cut(s)	238, 812, 1847, 3538, 3623, 4067
AsuC2I	CCSGG	1 cut(s)	3395
AsuHPI	GGTGA	3 cut(s)	558, 2901, 4094
AsuII	TTCGAA	1 cut(s)	96
AvaI	CYCGRG	2 cut(s)	1476, 4397
AvaII	GGWCC	3 cut(s)	1847, 3623, 4067
AvrII	CCTAGG	1 cut(s)	1757
BaeGI	GKGCMC	1 cut(s)	986
BaeI	ACNNNNGTAYC	6 cut(s)	2816, 2849, 3014, 3047, 4269, 4302
BalI	TGGCCA	2 cut(s)	213, 3084
BamHI	GGATCC	2 cut(s)	2345, 3397
BanI	GGYRCC	2 cut(s)	2090, 3782
BanII	GRGCYC	1 cut(s)	2411
BbsI	GAAGAC	4 cut(s)	669, 840, 4062, 4187
Bbv12I	GWGCWC	3 cut(s)	986, 1489, 2411
BbvCI	CCTCAGC	1 cut(s)	694
BcgI	CGANNNNNNTGC	2 cut(s)	3839, 3873
BciT130I	CCWGG	6 cut(s)	407, 647, 1737, 2838, 3375, 3882
BciVI	GTATCC	4 cut(s)	1115, 2844, 3323, 3412
BclI	TGATCA	4 cut(s)	187, 1044, 1933, 2893
BcnI	CCSGG	1 cut(s)	3395
BcoDI	GTCTC	8 cut(s)	11, 592, 1479, 1539, 2132, 3333, 3813, 4176
BcuI	ACTAGT	3 cut(s)	1840, 2161, 4282
BfmI	CTRYAG	1 cut(s)	2001
BfoI	RGCGCY	1 cut(s)	4397
BfuAI	ACCTGC	3 cut(s)	1994, 2892, 3303
BfuI	GTATCC	4 cut(s)	1115, 2844, 3323, 3412
BglII	AGATCT	1 cut(s)	1084
BlnI	CCTAGG	1 cut(s)	1757
BlpI	GCTNAGC	2 cut(s)	3602, 3713
BmcAI	AGTACT	2 cut(s)	1577, 2801
Bme1390I	CCNGG	7 cut(s)	407, 647, 1737, 2838, 3375, 3395, 3882
Bme18I	GGWCC	3 cut(s)	1847, 3623, 4067
BmeT110I	CYCGRG	2 cut(s)	1476, 4397
BmgBI	CACGTC	1 cut(s)	2188
BmgT120I	GGNCC	6 cut(s)	238, 812, 1847, 3538, 3623, 4067
BmiI	GGNNCC	7 cut(s)	240, 2092, 2347, 3399, 3625, 3784, 4068
BmrFI	CCNGG	7 cut(s)	407, 647, 1737, 2838, 3375, 3395, 3882
BoxI	GACNNNNGTC	1 cut(s)	339
BpiI	GAAGAC	4 cut(s)	669, 840, 4062, 4187
BplI	GAGNNNNNCTC	2 cut(s)	1536, 1568
BpmI	CTGGAG	8 cut(s)	358, 974, 1674, 1695, 1758, 3059, 3357, 3903
Bpu10I	CCTNAGC	2 cut(s)	321, 694
Bpu1102I	GCTNAGC	2 cut(s)	3602, 3713
Bpu14I	TTCGAA	1 cut(s)	96
BpuEI	CTTGAG	4 cut(s)	1109, 2567, 2813, 3918
BpuMI	CCSGG	1 cut(s)	3395
BsaAI	YACGTR	1 cut(s)	4214
BsaBI	GATNNNNATC	6 cut(s)	40, 170, 1126, 1935, 2489, 3876
BsaHI	GRCGYC	1 cut(s)	2655
BsaI	GGTCTC	1 cut(s)	1539
BsaJI	CCNNGG	6 cut(s)	597, 645, 1735, 1757, 2751, 3374
BsaWI	WCCGGW	3 cut(s)	1289, 1434, 3620
BsaXI	ACNNNNNCTCC	8 cut(s)	1668, 1698, 1801, 1831, 3177, 3207, 3810, 3840
Bsc4I	CCNNNNNNNGG	1 cut(s)	3651
Bse3DI	GCAATG	4 cut(s)	180, 1016, 3519, 3973
Bse8I	GATNNNNATC	6 cut(s)	40, 170, 1126, 1935, 2489, 3876
BseBI	CCWGG	6 cut(s)	407, 647, 1737, 2838, 3375, 3882
BseDI	CCNNGG	6 cut(s)	597, 645, 1735, 1757, 2751, 3374
BseJI	GATNNNNATC	6 cut(s)	40, 170, 1126, 1935, 2489, 3876
BseLI	CCNNNNNNNGG	1 cut(s)	3651
BseMI	GCAATG	4 cut(s)	180, 1016, 3519, 3973
BseMII	CTCAG	7 cut(s)	708, 2281, 2430, 2733, 3616, 3782, 4023
BseRI	GAGGAG	2 cut(s)	992, 1412
BseSI	GKGCMC	1 cut(s)	986
BseYI	CCCAGC	1 cut(s)	143
BsgI	GTGCAG	3 cut(s)	1895, 2292, 3564
Bsh1236I	CGCG	1 cut(s)	599
BshFI	GGCC	8 cut(s)	213, 239, 814, 1762, 2105, 3084, 3539, 3752
BshNI	GGYRCC	2 cut(s)	2090, 3782
BsiHKAI	GWGCWC	3 cut(s)	986, 1489, 2411
BsiHKCI	CYCGRG	2 cut(s)	1476, 4397
BsiSI	CCGG	4 cut(s)	1290, 1435, 3395, 3621
BslFI	GGGAC	5 cut(s)	53, 1494, 1541, 2733, 3028
BslI	CCNNNNNNNGG	1 cut(s)	3651
BsmAI	GTCTC	8 cut(s)	11, 592, 1479, 1539, 2132, 3333, 3813, 4176
BsmFI	GGGAC	5 cut(s)	53, 1494, 1541, 2733, 3028
BsmI	GAATGC	4 cut(s)	427, 473, 964, 2049
BsnI	GGCC	8 cut(s)	213, 239, 814, 1762, 2105, 3084, 3539, 3752
Bso31I	GGTCTC	1 cut(s)	1539
BsoBI	CYCGRG	2 cut(s)	1476, 4397
Bsp119I	TTCGAA	1 cut(s)	96
Bsp1286I	GDGCHC	3 cut(s)	986, 1489, 2411
Bsp1720I	GCTNAGC	2 cut(s)	3602, 3713
BspANI	GGCC	8 cut(s)	213, 239, 814, 1762, 2105, 3084, 3539, 3752
BspCNI	CTCAG	7 cut(s)	707, 2280, 2431, 2734, 3615, 3781, 4024
BspFNI	CGCG	1 cut(s)	599
BspHI	TCATGA	1 cut(s)	387
BspLI	GGNNCC	7 cut(s)	240, 2092, 2347, 3399, 3625, 3784, 4068
BspMAI	CTGCAG	1 cut(s)	2005
BspMI	ACCTGC	3 cut(s)	1994, 2892, 3303
BspQI	GCTCTTC	1 cut(s)	675
BspT104I	TTCGAA	1 cut(s)	96
BspT107I	GGYRCC	2 cut(s)	2090, 3782
BspTNI	GGTCTC	1 cut(s)	1539
BsrDI	GCAATG	4 cut(s)	180, 1016, 3519, 3973
BssECI	CCNNGG	6 cut(s)	597, 645, 1735, 1757, 2751, 3374
BssNAI	GTATAC	1 cut(s)	310
BssNI	GRCGYC	1 cut(s)	2655
BssT1I	CCWWGG	2 cut(s)	1757, 2751
Bst1107I	GTATAC	1 cut(s)	310
Bst2UI	CCWGG	6 cut(s)	407, 647, 1737, 2838, 3375, 3882
Bst6I	CTCTTC	1 cut(s)	675
BstACI	GRCGYC	1 cut(s)	2655
BstBAI	YACGTR	1 cut(s)	4214
BstBI	TTCGAA	1 cut(s)	96
BstC8I	GCNNGC	2 cut(s)	3058, 3787
BstDSI	CCRYGG	1 cut(s)	597
BstFNI	CGCG	1 cut(s)	599
BstH2I	RGCGCY	1 cut(s)	4397
BstHHI	GCGC	2 cut(s)	929, 4396
BstMAI	GTCTC	8 cut(s)	11, 592, 1479, 1539, 2132, 3333, 3813, 4176
BstMWI	GCNNNNNNNGC	8 cut(s)	865, 935, 1676, 2771, 2860, 3090, 3641, 3938
BstNI	CCWGG	6 cut(s)	407, 647, 1737, 2838, 3375, 3882
BstNSI	RCATGY	4 cut(s)	689, 3764, 4266, 4292
BstPAI	GACNNNNGTC	1 cut(s)	339
BstSCI	CCNGG	7 cut(s)	405, 645, 1735, 2836, 3373, 3393, 3880
BstSFI	CTRYAG	1 cut(s)	2001
BstSLI	GKGCMC	1 cut(s)	986
BstSNI	TACGTA	1 cut(s)	4214
BstUI	CGCG	1 cut(s)	599
BstV2I	GAAGAC	4 cut(s)	669, 840, 4062, 4187
BstX2I	RGATCY	5 cut(s)	1084, 1606, 2206, 2345, 3397
BstXI	CCANNNNNNTGG	3 cut(s)	351, 3363, 4051
BstYI	RGATCY	5 cut(s)	1084, 1606, 2206, 2345, 3397
BstZ17I	GTATAC	1 cut(s)	310
BsuI	GTATCC	4 cut(s)	1115, 2844, 3323, 3412
BsuRI	GGCC	8 cut(s)	213, 239, 814, 1762, 2105, 3084, 3539, 3752
BtgI	CCRYGG	1 cut(s)	597
BtrI	CACGTC	1 cut(s)	2188
BtsI	GCAGTG	2 cut(s)	9, 132
BveI	ACCTGC	3 cut(s)	1994, 2892, 3303
Cac8I	GCNNGC	2 cut(s)	3058, 3787
CaiI	CAGNNNCTG	4 cut(s)	2009, 3792, 3911, 4031
CciI	TCATGA	1 cut(s)	387
CfoI	GCGC	2 cut(s)	929, 4396
Cfr13I	GGNCC	6 cut(s)	238, 812, 1847, 3538, 3623, 4067
Cfr42I	CCGCGG	1 cut(s)	600
CseI	GACGC	1 cut(s)	2892
CspCI	CAANNNNNGTGG	2 cut(s)	1288, 1323
DraI	TTTAAA	1 cut(s)	4159
DrdI	GACNNNNNNGTC	1 cut(s)	1700
DseDI	GACNNNNNNGTC	1 cut(s)	1700
EaeI	YGGCCR	2 cut(s)	211, 3082
Eam1104I	CTCTTC	1 cut(s)	675
EarI	CTCTTC	1 cut(s)	675
EciI	GGCGGA	1 cut(s)	583
Ecl136II	GAGCTC	1 cut(s)	2409
Eco105I	TACGTA	1 cut(s)	4214
Eco130I	CCWWGG	2 cut(s)	1757, 2751
Eco147I	AGGCCT	1 cut(s)	2105
Eco24I	GRGCYC	1 cut(s)	2411
Eco31I	GGTCTC	1 cut(s)	1539
Eco32I	GATATC	2 cut(s)	55, 618
Eco47I	GGWCC	3 cut(s)	1847, 3623, 4067
Eco53kI	GAGCTC	1 cut(s)	2409
Eco57I	CTGAAG	3 cut(s)	1759, 2220, 2366
Eco88I	CYCGRG	2 cut(s)	1476, 4397
EcoICRI	GAGCTC	1 cut(s)	2409
EcoO109I	RGGNCCY	1 cut(s)	3538
EcoRI	GAATTC	2 cut(s)	98, 1404
EcoRII	CCWGG	6 cut(s)	405, 645, 1735, 2836, 3373, 3880
EcoRV	GATATC	2 cut(s)	55, 618
EcoT14I	CCWWGG	2 cut(s)	1757, 2751
EcoT22I	ATGCAT	2 cut(s)	427, 1657
EcoT38I	GRGCYC	1 cut(s)	2411
ErhI	CCWWGG	2 cut(s)	1757, 2751
FalI	AAGNNNNNCTT	6 cut(s)	1947, 1979, 2053, 2085, 4305, 4337
FaqI	GGGAC	5 cut(s)	53, 1494, 1541, 2733, 3028
FauNDI	CATATG	2 cut(s)	931, 2952
FbaI	TGATCA	4 cut(s)	187, 1044, 1933, 2893
FblI	GTMKAC	2 cut(s)	287, 309
FriOI	GRGCYC	1 cut(s)	2411
FspAI	RTGCGCAY	1 cut(s)	928
FspI	TGCGCA	1 cut(s)	928
GlaI	GCGC	2 cut(s)	928, 4395
GsaI	CCCAGC	1 cut(s)	147
GsuI	CTGGAG	8 cut(s)	358, 974, 1674, 1695, 1758, 3059, 3357, 3903
HaeII	RGCGCY	1 cut(s)	4397
HaeIII	GGCC	8 cut(s)	213, 239, 814, 1762, 2105, 3084, 3539, 3752
HapII	CCGG	4 cut(s)	1290, 1435, 3395, 3621
HgaI	GACGC	1 cut(s)	2892
HhaI	GCGC	2 cut(s)	929, 4396
Hin1I	GRCGYC	1 cut(s)	2655
Hin6I	GCGC	2 cut(s)	927, 4394
HinP1I	GCGC	2 cut(s)	927, 4394
HincII	GTYRAC	2 cut(s)	2992, 3891
HindII	GTYRAC	2 cut(s)	2992, 3891
HindIII	AAGCTT	5 cut(s)	765, 1896, 2235, 3710, 4127
HpaII	CCGG	4 cut(s)	1290, 1435, 3395, 3621
HphI	GGTGA	3 cut(s)	558, 2901, 4094
Hpy166II	GTNNAC	7 cut(s)	207, 288, 310, 984, 2992, 3891, 4217
Hpy8I	GTNNAC	7 cut(s)	207, 288, 310, 984, 2992, 3891, 4217
Hpy99I	CGWCG	2 cut(s)	3270, 3363
HpyCH4IV	ACGT	7 cut(s)	2187, 2241, 2655, 2813, 2994, 3361, 4213
HpyF10VI	GCNNNNNNNGC	8 cut(s)	865, 935, 1676, 2771, 2860, 3090, 3641, 3938
HpySE526I	ACGT	7 cut(s)	2187, 2241, 2655, 2813, 2994, 3361, 4213
Hsp92I	GRCGYC	1 cut(s)	2655
HspAI	GCGC	2 cut(s)	927, 4394
Ksp22I	TGATCA	4 cut(s)	187, 1044, 1933, 2893
KspI	CCGCGG	1 cut(s)	600
LguI	GCTCTTC	1 cut(s)	675
LmnI	GCTCC	9 cut(s)	317, 1427, 1676, 1737, 2406, 3166, 3376, 3548, 4106
MaeII	ACGT	7 cut(s)	2187, 2241, 2655, 2813, 2994, 3361, 4213
MfeI	CAATTG	3 cut(s)	2649, 4176, 4199
MflI	RGATCY	5 cut(s)	1084, 1606, 2206, 2345, 3397
MhlI	GDGCHC	3 cut(s)	986, 1489, 2411
MlsI	TGGCCA	2 cut(s)	213, 3084
MluNI	TGGCCA	2 cut(s)	213, 3084
MlyI	GAGTC	9 cut(s)	13, 349, 1100, 1355, 1688, 2755, 3050, 3815, 4003
MmeI	TCCRAC	4 cut(s)	111, 3454, 3810, 4309
Mox20I	TGGCCA	2 cut(s)	213, 3084
Mph1103I	ATGCAT	2 cut(s)	427, 1657
MroXI	GAANNNNTTC	5 cut(s)	92, 1056, 1149, 2494, 4317
MscI	TGGCCA	2 cut(s)	213, 3084
MslI	CAYNNNNRTG	5 cut(s)	2268, 3142, 3406, 3561, 3664
Msp20I	TGGCCA	2 cut(s)	213, 3084
MspA1I	CMGCKG	3 cut(s)	599, 3908, 4309
MspI	CCGG	4 cut(s)	1290, 1435, 3395, 3621
MspR9I	CCNGG	7 cut(s)	407, 647, 1737, 2838, 3375, 3395, 3882
MunI	CAATTG	3 cut(s)	2649, 4176, 4199
Mva1269I	GAATGC	4 cut(s)	427, 473, 964, 2049
MvaI	CCWGG	6 cut(s)	407, 647, 1737, 2838, 3375, 3882
MvnI	CGCG	1 cut(s)	599
MwoI	GCNNNNNNNGC	8 cut(s)	865, 935, 1676, 2771, 2860, 3090, 3641, 3938
NciI	CCSGG	1 cut(s)	3395
NdeI	CATATG	2 cut(s)	931, 2952
NlaIV	GGNNCC	7 cut(s)	240, 2092, 2347, 3399, 3625, 3784, 4068
NmuCI	GTSAC	5 cut(s)	1216, 3494, 3995, 4082, 4180
NsbI	TGCGCA	1 cut(s)	928
NsiI	ATGCAT	2 cut(s)	427, 1657
NspI	RCATGY	4 cut(s)	689, 3764, 4266, 4292
NspV	TTCGAA	1 cut(s)	96
PaeR7I	CTCGAG	1 cut(s)	4397
PagI	TCATGA	1 cut(s)	387
PaqCI	CACCTGC	1 cut(s)	1994
PceI	AGGCCT	1 cut(s)	2105
PciI	ACATGT	1 cut(s)	4262
PciSI	GCTCTTC	1 cut(s)	675
PctI	GAATGC	4 cut(s)	427, 473, 964, 2049
PdmI	GAANNNNTTC	5 cut(s)	92, 1056, 1149, 2494, 4317
PfeI	GAWTC	9 cut(s)	641, 1499, 2490, 2703, 3118, 3188, 3390, 3611, 4235
PfoI	TCCNGGA	4 cut(s)	405, 2836, 3393, 3880
PleI	GAGTC	9 cut(s)	12, 348, 1099, 1354, 1688, 2754, 3049, 3815, 4002
PpsI	GAGTC	9 cut(s)	12, 348, 1099, 1354, 1688, 2754, 3049, 3815, 4002
Ppu21I	YACGTR	1 cut(s)	4214
PscI	ACATGT	1 cut(s)	4262
PshAI	GACNNNNGTC	1 cut(s)	339
PshBI	ATTAAT	1 cut(s)	840
PsiI	TTATAA	1 cut(s)	278
Psp124BI	GAGCTC	1 cut(s)	2411
Psp6I	CCWGG	6 cut(s)	405, 645, 1735, 2836, 3373, 3880
PspFI	CCCAGC	1 cut(s)	143
PspGI	CCWGG	6 cut(s)	405, 645, 1735, 2836, 3373, 3880
PspN4I	GGNNCC	7 cut(s)	240, 2092, 2347, 3399, 3625, 3784, 4068
PspPI	GGNCC	6 cut(s)	238, 812, 1847, 3538, 3623, 4067
PstI	CTGCAG	1 cut(s)	2005
PstNI	CAGNNNCTG	4 cut(s)	2009, 3792, 3911, 4031
PsuI	RGATCY	5 cut(s)	1084, 1606, 2206, 2345, 3397
PvuII	CAGCTG	2 cut(s)	3908, 4309
RseI	CAYNNNNRTG	5 cut(s)	2268, 3142, 3406, 3561, 3664
SacI	GAGCTC	1 cut(s)	2411
SacII	CCGCGG	1 cut(s)	600
SapI	GCTCTTC	1 cut(s)	675
Sau96I	GGNCC	6 cut(s)	238, 812, 1847, 3538, 3623, 4067
ScaI	AGTACT	2 cut(s)	1577, 2801
SchI	GAGTC	9 cut(s)	13, 349, 1100, 1355, 1688, 2755, 3050, 3815, 4003
ScrFI	CCNGG	7 cut(s)	407, 647, 1737, 2838, 3375, 3395, 3882
SduI	GDGCHC	3 cut(s)	986, 1489, 2411
SfcI	CTRYAG	1 cut(s)	2001
Sfr274I	CTCGAG	1 cut(s)	4397
Sfr303I	CCGCGG	1 cut(s)	600
SfuI	TTCGAA	1 cut(s)	96
SgrBI	CCGCGG	1 cut(s)	600
SinI	GGWCC	3 cut(s)	1847, 3623, 4067
SlaI	CTCGAG	1 cut(s)	4397
SmiMI	CAYNNNNRTG	5 cut(s)	2268, 3142, 3406, 3561, 3664
SmlI	CTYRAG	5 cut(s)	1088, 2546, 2828, 3933, 4397
SmoI	CTYRAG	5 cut(s)	1088, 2546, 2828, 3933, 4397
SnaBI	TACGTA	1 cut(s)	4214
SpeI	ACTAGT	3 cut(s)	1840, 2161, 4282
SseBI	AGGCCT	1 cut(s)	2105
SspI	AATATT	2 cut(s)	350, 4167
SstI	GAGCTC	1 cut(s)	2411
StuI	AGGCCT	1 cut(s)	2105
StyD4I	CCNGG	7 cut(s)	405, 645, 1735, 2836, 3373, 3393, 3880
StyI	CCWWGG	2 cut(s)	1757, 2751
TaiI	ACGT	7 cut(s)	2190, 2244, 2658, 2816, 2997, 3364, 4216
TatI	WGTACW	5 cut(s)	1575, 2155, 2799, 2824, 4265
TauI	GCSGC	2 cut(s)	599, 1829
TfiI	GAWTC	9 cut(s)	641, 1499, 2490, 2703, 3118, 3188, 3390, 3611, 4235
TseFI	GTSAC	5 cut(s)	1216, 3494, 3995, 4082, 4180
Tsp45I	GTSAC	5 cut(s)	1216, 3494, 3995, 4082, 4180
TspGWI	ACGGA	1 cut(s)	330
VneI	GTGCAC	1 cut(s)	982
VpaK11BI	GGWCC	3 cut(s)	1847, 3623, 4067
VspI	ATTAAT	1 cut(s)	840
XbaI	TCTAGA	2 cut(s)	3121, 3386
XceI	RCATGY	4 cut(s)	689, 3764, 4266, 4292
XcmI	CCANNNNNNNNNTGG	1 cut(s)	479
XhoI	CTCGAG	1 cut(s)	4397
XmaJI	CCTAGG	1 cut(s)	1757
XmiI	GTMKAC	2 cut(s)	287, 309
XmnI	GAANNNNTTC	5 cut(s)	92, 1056, 1149, 2494, 4317
ZraI	GACGTC	1 cut(s)	2656
ZrmI	AGTACT	2 cut(s)	1577, 2801
Zsp2I	ATGCAT	2 cut(s)	427, 1657

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.