Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0001909

Physical Location & Seq

Genomic Coordinates Forward (+)

16120223 .. 16128487

Gene Structure 8265 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g18930.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5052 bp

ATGAACCACTTTTTCTTTCTTTCAGAATCTCACTCGCAAATTATTGGATGTTCTACATATATTTGGAAGATCAAAAAAATCTCCAAGCTTGAGCTGGAATTCTCCTCCAACTTCGATATGGGTCTCACTGCTCTTGACAGTCCTCTTACTGAATCTCATTCTCCCCAAGATATCAAGCATTTAATCCCCAGAAGGTATCAAGAGAAAGTGTTGGAAATTGCAAAGCGTAAGAACACAATTGCAGTTCTGGAAACTGGAGCTGGAAAGACTATGATTGCTGTGTTGCTCATCAAACACATTGCTGATCAACATATCATCAAATCCACTGGCCACAAGAAGTTGATAATTTTCATGGCCCCAACAGTTCATCTTGTCATTCAGCAATCTGAGGTTATAGAAAAGTGTACAACTCTTGAAGTGGGAGTGTACTACGGAGCTAAGGGCGTGGATGACTGGAGTAGAGAATGTTGGGAGGCGGAAGTGAAAACACATGATATAATGGTCATGACACCTCAAATTCTCCTGGATGCTTTGAGGAATGCATTTTTGAGGATAGATATGATTTGCTTGATGGTCTTCGATGAATGCCACCGTGCTTCTGGCAATCATCCCTACGCGAGAATAATGAAGGAATTTCATCACAAATCAGGAAACAAGCCAAAGATTTTTGGAATGACAGCATCACCGGTCATTAAAAAAGGTGTCTCATCCGCCGTGGACTGTGAGGATCAGATATCACAACTTGAAAGCGTCTTGGATTCCCAGGTTTATACTGTAGAAGACAGGACAGAGATTGAAGAGCATGTTCCCTCAGCAGTTCAAAGTTGTAAACTTTACAACCCAACATGGTTAACCTGTGAGGATTTGAAAGAAAAGATGGAAGCTTTATTGTCAAAGTTTGATTCTTCTATCAAGTTAAAGTTGCACGGGCCAGAGAAAATAGAGTATGAAGACATTAATGACAAAATCAAGGCGCTACGAAAGCGGTTGTCCAATGACTACATGAAAATCTTGTATTGCCTTGATGATCTTGGCATCATATGCGCATATGAGGCTGTGAAAATCTGTCTGGAGAATGCCCCTGATGTCAATGAGGAGTGCACATTTTATAGAGAAAGCTCTTTGCAGTGCAGATGCTTTCTTGATGAAGTGTTAAGTATGATAGGTGAATCACTTAAAGAAGGTCATAAACTTCATACAGATTTTGAGTTGGACTATAAAAAGGCACACGAAGTGGGCTACATATCTCCCAAGTTATATGAACTTCTTCAAATTTTCCACTCATTTGGGAGCGATAGGCCAGTCCTGTGCCTTATTTTTGTAGAGAGAATTATCACAGCCAAAGTAATTCAAAGATATGTGAAGAAAGTGCCATGCTTATCTCATTTCACAGCTTCATATTTGACCGGAAGTACCACATCAGTTGATGCACTTGCACCGAAATTGCAGAAGGAAATTTTGGAGTCATTTTACACTGGGAAGGTCAATCTACTATTTGCCACTGATGTAGTTGAGGAGGGAATTCATGTCCAGAACTGCTCCTATGTGATCCGATTTGATCTTCCCAAGACAGTTCGTAGCTATGTCCAGTCTCGGGGACGAGCAAGGAAAGATGATTCTCAATTTATCATCATGCTTGAACGGGGAAATAAGAAGCAAAGAGACCATCTCTGTGACATCATCAGGAGTGAGCACTCAATGACAGATACATCGAGGAATCGAGATCCTGATGTATGCAGTTTAAGAGCTTGTAACTTTGAGGATGCAAATGCATACTATGTTGAAGCTACTGCAGCATCAGTTACTCCAGACTCTAGTGTCAACCTAACATATCGGTACTGCGAGAAGCTCCCTGGAGATAGGTACTTTATTCCAAGGCCAAATTTTCACTTCAGACTATTTGAAGGGTTGTATCAGTGTGAAATAACATTACCTCCAAATGCGGCTTTTCAAACTATAGTTGGTCCTATGAGTAGAAACAGTCATTCATCAAAGCAGCTTGTATGCTTGGAAGCTTGTAGAAAGTTGCATCAAATGGGTGCTTTGGATGATCATCTTCTCCCACTTGTTGAAGTACCTCCTGAAAATGAAGTGAATGTGAAAAGCAAAGAATTATCTGCAGGAACTACAAAACGTAAAGAGTTACATGGGACGTCTTGCATTCGTGCATTAAGCGGAACTTGGGGAGAGAAACTTGATGGTGCCAATTTTGAGGCCTACAAATTTGAGTTCTCGTGTAATGTTGAAGAGACATATTCAGGATTTGTTCTATTAGTCGAGTCGAAACTTGATGATGACGTTGGAAATATTGAAGTGGATCTCTACTTGATTGCAAAGATGGTCAAAGCTTATGTTTCTTCATGTGGAAAGGTGCATCTCACTGCAGATCAGATAAAGCAAGCTATGAGCTTTCAAGAATTTTTCTGTAATGGTTTGTTTGGGAGATTGTTTCTTGGAAACGAACCAGAAGGGACTGAAAGAAATTTTTTACTTCAGACTGAAATAAAATCACTATGGAGCTCATCATACAGCTATTTGCTTTTACCGACTGAGGCATCGGAAAATTCAAATACGATATCTTGGAACATAGATTGGATAGGAATCTGTTCTTGTGCATATGTGGTGGAGTTTCTTAGGAAACACTATTCAAATGGTCTTCAGTATTTCAAAGGCGGTAGAGGAAATATATCACTTTCTAGGACTGGTTCATCCATGACAGATTGCAGCGCTCAAAATCTAGTCAACTTTGCTAATAGTTCGAATGATGTCAATAATCTTGCCAGTAATCTTGAAGATATGGTAGTGTTAGCTATCCACACCGGAAGAATCTACTCTATTGTTGAAGTTGTTAGTAGCATTTCTGCTGAGAGTCCCTTGGAAGAAAATGCTGATGTTGCGTCATCAGATTACACTTATGTCGAGTACTTCAAAAAAACGTATGGGATTGCACTCAAGTATCCAGGACAGCCTCTATTGCGGTTGAAGCAAAGTCATAATGCACATAACCTGCTTGTTGATCAAGGTGTGTCATCACCAGATGGTCTAGTTCAAGAAAAGCAACAAGCTCATGTTCATATGCCACCTGAAATCTTAGTCAGTATTGATATTCGGGTTGACGTTCTAAAGTCATTCTACTTGCTTCCATCATTAATGTACCGTCTGGAGTCACTGATGTTAGCCAGCCAGATTAGGGAAGAGATCAATGGCCAATCTAGCAACTTTTACATATCAAGCTCATTGATTCTGGAAGCACTCACAACACTTAGATGCTGTGAACATTTCTCATTGGAGCGTTTGGAGTTGCTAGGAGATTCAGTTCTCAAATATGCCGTGAGCTCCTACCTCTTTCTTAAATATCCCGAGAAACATGAAGGACAATTAACTGCTCGACGAACAAGAGCTATTTGCAACTCAAACCTGCATAAACTAGGAATAAAAAACAAGTTACAGGGATACATACGAGACAGTGCATTTGAACCACGGCGTTGGGCTGCTCCAGGGCAGCTTAGCAGATTCCCAGATCCTTGTCAATGTGGAGTGGATACTTTAAAAGTGCCCTTGGACAGTAAATTTCAGACTGAAAATGCTGTTAAGGTTGGAAAATTCTGTGATAAGGGTCACAGGTGGATGAACTCAAAAACAATTGCTGATTGTGTTGAGGCCCTTATAGGAGCTTATTATGTTGGTGGAGGATTATTTGCTGCACTTCATTTGATGAAGTGGCTTGGCATTGATTCTGAAACTGGACCCTCATTAGCTGTTGAAGCCATTACTAGGGCATCACTTCGATCATACAGTCCCAAAACTAATGAACTTGCTGACTTGGAGATGAAGCTTAGTTATGAATTTTCAGTCAAGGCCTTGTTACATGAAGCCATCACACATGCCTCTCAGCAAAAACTAGGTGCCAGCTACTGTTACCAGAGGCTTGAATTTCTAGGAGACTCGGTGTTGGATCTGCTAATTACACAGCATCTGTATCGCAGCCACATACATATTGATCCGGGAGTGTTGACTGATTTGCGGTCAGCTGCTGTTAGTAATGAAAACTTTGCTCAAGCTGCTATTAGACGAAACCTTCATCCACATCTTCAACACTGCTCAGGGTTACTACAGAGTCACATAACCGAGTATGAGACTAGGTTGCTTACAGAAGCTGAGAACAATACCAGTGCACTGGAAGACATCAAGGGTCCAAAGGCACTTGGTGACATGGTGGAAAGTATAGCAGGAGCTATACTAATTGATACAAAGCTTAATCTTGATGAAGTATGGAGGGTTTTTGAACCAATATTGTCTCCAATTGTGACCCCTTCTACTCTTCAATTGCATCCACTACGTAAACTGTCTGAATTATGTGACTCACTTGGGTACTTTGTGAAAGAAACATGTACTGAAGATGACACACTAGTGCATGTGGAACTAAGTTTGCAGCTGGAAGATGTTCTTTTGGTTGGAAATGGCTTTGACCGGAGCAGAAAAGCTGCGAAACATAAGGCTGCTCGTCAAGTGTTGATGGAGCTGGAGAAAAGCATAGAAAGGACAAAGCACTGTTCAGATATGGATGTTGACATCTGGAGCAACCTAAACGATGAAGATTCAGAGGCAATGGACCATGGACAGCAGAATGTGATGTCAATTCAATCATCTTCAGAGTCAAACACAATAGATCCTACCCAAGTCAATGACTTTTCTAATGAAGTACTCTTATGTCGGAACATTGCAATACCAGTTGTTGGTCCAATCAATACAAGGAAAGGTGAACCTCGAAAATCTCTCTTTGAGCTGTGTAAGAAATTGCAGTGGCCAATGCCTTCATTCACAACAACTGAGCAAAAGTCAAGAAAGCCAATTGAATTCGGTGAGGGTTGCGAAAAGAGAATAGGTTTCAGCAGCTTTCAATCAAAAATCAACTTGCACATTCCCAACTCAAGCACTATTGTATGCACAGGAGATCCAAGAGCTGATAAGAAGACATCCATGGATTCTGCAGTAGTTTCCATGCTTCACGAGCTTCAACGACAGAGAAAACTTGTCATTCTAGATTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1684

Amino Acids

189.87

Weight (kDa)

6.04

Isoelectric Point (pI)

43.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	63 - 229	1.6e-16	Type III restriction enzyme, res subunit
DEAD	PF00270	65 - 229	7.6e-19	DEAD/DEAH box helicase
Helicase_C	PF00271	431 - 535	7.2e-16	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	606 - 690	7.1e-23	Dicer dimerisation domain
PAZ	PF02170	922 - 1053	6.7e-12	PAZ domain
Ribonuclease_3	PF00636	1097 - 1230	3.2e-21	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1097 - 1154	2.4e-07	Ribonuclease-III-like
Ribonucleas_3_3	PF14622	1287 - 1416	1.9e-12	Ribonuclease-III-like
Ribonuclease_3	PF00636	1308 - 1417	1.3e-19	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001909)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920
fragaria_vesca	FvH4_2g07790 FvH4_2g18930 FvH4_2g18930 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780
malus_domestica	MD08G1046300.v1.1 MD10G1163700.v1.1 MD15G1047500.v1.1
prunus_persica	Prupe.1G401900_v2.0.a1 Prupe.1G401900_v2.0.a1 Prupe.8G202000_v2.0.a1 Prupe.8G202000_v2.0.a1
pyrus_communis	pycom08g03830 pycom08g03840 pycom15g04670
rosa_chinensis	RchiOBHm_Chr6g0284721 RchiOBHm_Chr6g0312121
rosa_laevigata	RLG00000010353 RLG00000012724
rosa_multiflora	Rmu_sc0004065.1_g000007 Rmu_sc0007474.1_g000021
rosa_roxburghii	Rroxscaffold_7G00157050 Rroxscaffold_7G00184440
rosa_rugosa	Rorug06G0165900 Rorug06G0392600 Rorug06G0392700 Rorug06G0392800
rosa_samantha	Rh4CG118000 Rh6AG279000 Rh6AG509700 Rh6BG279000 Rh6BG520600 Rh6CG281300 Rh6CG526600 Rh6DG274000 Rh6DG512000
rosa_wichuraiana	Rw6G023980 Rw6G044350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1817
AatII	GACGTC	1 cut(s)	2156
Acc16I	TGCGCA	1 cut(s)	1045
Acc36I	ACCTGC	2 cut(s)	3015, 3426
AccB1I	GGYRCC	2 cut(s)	2201, 3905
AccB7I	CCANNNNNTGG	1 cut(s)	4736
AccII	CGCG	1 cut(s)	617
AciI	CCGC	8 cut(s)	476, 711, 985, 1943, 2175, 2673, 2977, 4024
AclWI	GGATC	9 cut(s)	735, 1543, 1718, 2325, 3515, 3963, 3995, 4664, 4949
AcoI	YGGCCR	3 cut(s)	328, 3205, 4805
AcuI	CTGAAG	5 cut(s)	1876, 2477, 2642, 4416, 4635
AcyI	GRCGYC	1 cut(s)	2153
AdeI	CACNNNGTG	2 cut(s)	1234, 4208
AfeI	AGCGCT	1 cut(s)	2728
AfiI	CCNNNNNNNGG	4 cut(s)	1594, 3190, 3774, 4736
AflIII	ACRYGT	1 cut(s)	4388
AgeI	ACCGGT	1 cut(s)	685
AhlI	ACTAGT	1 cut(s)	4408
AjnI	CCWGG	5 cut(s)	522, 762, 1852, 2959, 3496
AjuI	GAANNNNNNNTTGG	2 cut(s)	1442, 1474
AleI	CACNNNNGTG	1 cut(s)	4409
AloI	GAACNNNNNNTCC	2 cut(s)	3300, 3332
Alw21I	GWGCWC	5 cut(s)	1103, 1695, 2522, 3338, 4177
Alw26I	GTCTC	9 cut(s)	128, 709, 1596, 1656, 2243, 3456, 3936, 4130, 4302
Alw44I	GTGCAC	2 cut(s)	1099, 4173
AlwI	GGATC	9 cut(s)	735, 1543, 1718, 2325, 3515, 3963, 3995, 4664, 4949
AlwNI	CAGNNNCTG	4 cut(s)	3915, 3976, 4034, 4157
Ama87I	CYCGRG	2 cut(s)	1593, 3359
Aor51HI	AGCGCT	1 cut(s)	2728
ApaLI	GTGCAC	2 cut(s)	1099, 4173
ArsI	GACNNNNNNTTYG	2 cut(s)	3455, 3487
AseI	ATTAAT	2 cut(s)	957, 3149
AsiGI	ACCGGT	1 cut(s)	685
Asp700I	GAANNNNTTC	3 cut(s)	1266, 2605, 4443
AspLEI	GCGC	3 cut(s)	976, 1046, 2729
AspS9I	GGNCC	8 cut(s)	355, 929, 1964, 3661, 3746, 4193, 4612, 4739
AsuC2I	CCSGG	1 cut(s)	4005
AsuHPI	GGTGA	6 cut(s)	675, 1178, 3024, 4220, 4772, 4874
AsuII	TTCGAA	1 cut(s)	2759
AvaI	CYCGRG	2 cut(s)	1593, 3359
AvaII	GGWCC	5 cut(s)	1964, 3746, 4193, 4612, 4739
BaeGI	GKGCMC	3 cut(s)	1103, 3558, 4177
BaeI	ACNNNNGTAYC	2 cut(s)	2939, 2972
BalI	TGGCCA	3 cut(s)	330, 3207, 4807
BanI	GGYRCC	2 cut(s)	2201, 3905
BanII	GRGCYC	2 cut(s)	2522, 3338
BauI	CACGAG	2 cut(s)	2233, 5009
BbsI	GAAGAC	6 cut(s)	568, 786, 957, 2648, 4188, 4979
Bbv12I	GWGCWC	5 cut(s)	1103, 1695, 2522, 3338, 4177
BbvCI	CCTCAGC	1 cut(s)	811
BccI	CCATC	9 cut(s)	565, 871, 1674, 2192, 2332, 3032, 3151, 3884, 4510
BceAI	ACGGC	3 cut(s)	698, 3314, 3497
BcgI	CGANNNNNNTGC	2 cut(s)	3962, 3996
BciT130I	CCWGG	5 cut(s)	524, 764, 1854, 2961, 3498
BciVI	GTATCC	3 cut(s)	2967, 3446, 3535
BclI	TGATCA	3 cut(s)	304, 2050, 3016
BcnI	CCSGG	1 cut(s)	4005
BcoDI	GTCTC	9 cut(s)	128, 709, 1596, 1656, 2243, 3456, 3936, 4130, 4302
BcuI	ACTAGT	1 cut(s)	4408
BfmI	CTRYAG	7 cut(s)	774, 1791, 1956, 2118, 2382, 4112, 4989
BfoI	RGCGCY	2 cut(s)	977, 2730
BfuAI	ACCTGC	2 cut(s)	3015, 3426
BfuI	GTATCC	3 cut(s)	2967, 3446, 3535
BlpI	GCTNAGC	1 cut(s)	3506
BmcAI	AGTACT	2 cut(s)	2924, 4704
Bme1390I	CCNGG	6 cut(s)	524, 764, 1854, 2961, 3498, 4005
Bme18I	GGWCC	5 cut(s)	1964, 3746, 4193, 4612, 4739
BmeT110I	CYCGRG	2 cut(s)	1593, 3359
BmgT120I	GGNCC	8 cut(s)	355, 929, 1964, 3661, 3746, 4193, 4612, 4739
BmiI	GGNNCC	5 cut(s)	357, 2203, 3748, 3907, 4194
BmrFI	CCNGG	6 cut(s)	524, 764, 1854, 2961, 3498, 4005
BmrI	ACTGGG	1 cut(s)	1485
BmuI	ACTGGG	1 cut(s)	1485
BpiI	GAAGAC	6 cut(s)	568, 786, 957, 2648, 4188, 4979
BplI	GAGNNNNNCTC	2 cut(s)	1653, 1685
BpmI	CTGGAG	9 cut(s)	276, 475, 1091, 1791, 1875, 3182, 3480, 4544, 4597
Bpu10I	CCTNAGC	3 cut(s)	438, 811, 4102
Bpu1102I	GCTNAGC	1 cut(s)	3506
Bpu14I	TTCGAA	1 cut(s)	2759
BpuEI	CTTGAG	4 cut(s)	110, 2936, 4041, 4915
BpuMI	CCSGG	1 cut(s)	4005
BsaAI	YACGTR	1 cut(s)	4340
BsaBI	GATNNNNATC	2 cut(s)	2052, 2600
BsaHI	GRCGYC	1 cut(s)	2153
BsaI	GGTCTC	2 cut(s)	128, 1656
BsaWI	WCCGGW	4 cut(s)	685, 1406, 2819, 4470
BsaXI	ACNNNNNCTCC	6 cut(s)	1918, 1948, 3300, 3330, 3933, 3963
Bsc4I	CCNNNNNNNGG	4 cut(s)	1594, 3190, 3774, 4736
Bse118I	RCCGGY	1 cut(s)	685
Bse3DI	GCAATG	3 cut(s)	297, 4614, 4719
Bse8I	GATNNNNATC	2 cut(s)	2052, 2600
BseBI	CCWGG	5 cut(s)	524, 764, 1854, 2961, 3498
BseJI	GATNNNNATC	2 cut(s)	2052, 2600
BseLI	CCNNNNNNNGG	4 cut(s)	1594, 3190, 3774, 4736
BseMI	GCAATG	3 cut(s)	297, 4614, 4719
BseMII	CTCAG	8 cut(s)	378, 825, 2541, 2856, 3905, 4116, 4149, 4821
BseRI	GAGGAG	3 cut(s)	94, 1109, 1529
BseSI	GKGCMC	3 cut(s)	1103, 3558, 4177
BsgI	GTGCAG	2 cut(s)	1150, 3687
Bsh1236I	CGCG	1 cut(s)	617
BshNI	GGYRCC	2 cut(s)	2201, 3905
BshTI	ACCGGT	1 cut(s)	685
BsiHKAI	GWGCWC	5 cut(s)	1103, 1695, 2522, 3338, 4177
BsiHKCI	CYCGRG	2 cut(s)	1593, 3359
BsiSI	CCGG	5 cut(s)	686, 1407, 2820, 4004, 4471
BslFI	GGGAC	5 cut(s)	1611, 2164, 2485, 2856, 3783
BslI	CCNNNNNNNGG	4 cut(s)	1594, 3190, 3774, 4736
BsmAI	GTCTC	9 cut(s)	128, 709, 1596, 1656, 2243, 3456, 3936, 4130, 4302
BsmFI	GGGAC	5 cut(s)	1611, 2164, 2485, 2856, 3783
BsmI	GAATGC	4 cut(s)	544, 590, 1081, 2160
Bso31I	GGTCTC	2 cut(s)	128, 1656
BsoBI	CYCGRG	2 cut(s)	1593, 3359
Bsp119I	TTCGAA	1 cut(s)	2759
Bsp1286I	GDGCHC	6 cut(s)	1103, 1695, 2522, 3338, 3558, 4177
Bsp1407I	TGTACA	1 cut(s)	404
Bsp1720I	GCTNAGC	1 cut(s)	3506
Bsp19I	CCATGG	2 cut(s)	4615, 4980
BspACI	CCGC	8 cut(s)	476, 711, 985, 1943, 2175, 2673, 2977, 4024
BspCNI	CTCAG	8 cut(s)	379, 824, 2542, 2857, 3904, 4115, 4150, 4822
BspFNI	CGCG	1 cut(s)	617
BspHI	TCATGA	1 cut(s)	504
BspLI	GGNNCC	5 cut(s)	357, 2203, 3748, 3907, 4194
BspMAI	CTGCAG	4 cut(s)	1795, 2122, 2386, 4993
BspMI	ACCTGC	2 cut(s)	3015, 3426
BspPI	GGATC	9 cut(s)	735, 1543, 1718, 2325, 3515, 3963, 3995, 4664, 4949
BspQI	GCTCTTC	1 cut(s)	792
BspT104I	TTCGAA	1 cut(s)	2759
BspT107I	GGYRCC	2 cut(s)	2201, 3905
BspTNI	GGTCTC	2 cut(s)	128, 1656
BsrDI	GCAATG	3 cut(s)	297, 4614, 4719
BsrFI	RCCGGY	1 cut(s)	685
BsrGI	TGTACA	1 cut(s)	404
BssAI	RCCGGY	1 cut(s)	685
BssNI	GRCGYC	1 cut(s)	2153
BssSI	CACGAG	2 cut(s)	2233, 5009
BssT1I	CCWWGG	5 cut(s)	1874, 2874, 3558, 4615, 4980
Bst2BI	CACGAG	2 cut(s)	2233, 5009
Bst2UI	CCWGG	5 cut(s)	524, 764, 1854, 2961, 3498
Bst6I	CTCTTC	4 cut(s)	792, 2241, 3189, 4326
BstACI	GRCGYC	1 cut(s)	2153
BstAPI	GCANNNNNTGC	1 cut(s)	1041
BstAUI	TGTACA	1 cut(s)	404
BstBAI	YACGTR	1 cut(s)	4340
BstBI	TTCGAA	1 cut(s)	2759
BstC8I	GCNNGC	3 cut(s)	2400, 3181, 3910
BstDSI	CCRYGG	4 cut(s)	714, 3479, 4615, 4980
BstFNI	CGCG	1 cut(s)	617
BstH2I	RGCGCY	2 cut(s)	977, 2730
BstHHI	GCGC	3 cut(s)	976, 1046, 2729
BstMAI	GTCTC	9 cut(s)	128, 709, 1596, 1656, 2243, 3456, 3936, 4130, 4302
BstNI	CCWGG	5 cut(s)	524, 764, 1854, 2961, 3498
BstNSI	RCATGY	4 cut(s)	806, 3887, 4392, 4418
BstSCI	CCNGG	6 cut(s)	522, 762, 1852, 2959, 3496, 4003
BstSFI	CTRYAG	7 cut(s)	774, 1791, 1956, 2118, 2382, 4112, 4989
BstSLI	GKGCMC	3 cut(s)	1103, 3558, 4177
BstSNI	TACGTA	1 cut(s)	4340
BstUI	CGCG	1 cut(s)	617
BstV2I	GAAGAC	6 cut(s)	568, 786, 957, 2648, 4188, 4979
BstX2I	RGATCY	6 cut(s)	1723, 2317, 3520, 3955, 4669, 4954
BstXI	CCANNNNNNTGG	3 cut(s)	1286, 3486, 4177
BstYI	RGATCY	6 cut(s)	1723, 2317, 3520, 3955, 4669, 4954
BsuI	GTATCC	3 cut(s)	2967, 3446, 3535
BtgI	CCRYGG	4 cut(s)	714, 3479, 4615, 4980
BtsI	GCAGTG	5 cut(s)	126, 1133, 2379, 4096, 4808
BveI	ACCTGC	2 cut(s)	3015, 3426
Cac8I	GCNNGC	3 cut(s)	2400, 3181, 3910
CaiI	CAGNNNCTG	4 cut(s)	3915, 3976, 4034, 4157
CciI	TCATGA	1 cut(s)	504
CfoI	GCGC	3 cut(s)	976, 1046, 2729
Cfr10I	RCCGGY	1 cut(s)	685
Cfr13I	GGNCC	8 cut(s)	355, 929, 1964, 3661, 3746, 4193, 4612, 4739
CseI	GACGC	2 cut(s)	739, 2886
CspAI	ACCGGT	1 cut(s)	685
CspCI	CAANNNNNGTGG	2 cut(s)	1405, 1440
DraI	TTTAAA	1 cut(s)	3549
DraIII	CACNNNGTG	2 cut(s)	1234, 4208
DrdI	GACNNNNNNGTC	1 cut(s)	1817
DseDI	GACNNNNNNGTC	1 cut(s)	1817
EaeI	YGGCCR	3 cut(s)	328, 3205, 4805
Eam1104I	CTCTTC	4 cut(s)	792, 2241, 3189, 4326
EarI	CTCTTC	4 cut(s)	792, 2241, 3189, 4326
EciI	GGCGGA	2 cut(s)	491, 700
Ecl136II	GAGCTC	2 cut(s)	2520, 3336
Eco105I	TACGTA	1 cut(s)	4340
Eco130I	CCWWGG	5 cut(s)	1874, 2874, 3558, 4615, 4980
Eco147I	AGGCCT	2 cut(s)	2216, 3860
Eco24I	GRGCYC	2 cut(s)	2522, 3338
Eco31I	GGTCTC	2 cut(s)	128, 1656
Eco32I	GATATC	3 cut(s)	172, 735, 2577
Eco47I	GGWCC	5 cut(s)	1964, 3746, 4193, 4612, 4739
Eco47III	AGCGCT	1 cut(s)	2728
Eco53kI	GAGCTC	2 cut(s)	2520, 3336
Eco57I	CTGAAG	5 cut(s)	1876, 2477, 2642, 4416, 4635
Eco88I	CYCGRG	2 cut(s)	1593, 3359
EcoICRI	GAGCTC	2 cut(s)	2520, 3336
EcoO109I	RGGNCCY	1 cut(s)	3661
EcoRI	GAATTC	3 cut(s)	98, 1521, 4856
EcoRII	CCWGG	5 cut(s)	522, 762, 1852, 2959, 3496
EcoRV	GATATC	3 cut(s)	172, 735, 2577
EcoT14I	CCWWGG	5 cut(s)	1874, 2874, 3558, 4615, 4980
EcoT22I	ATGCAT	2 cut(s)	544, 1774
EcoT38I	GRGCYC	2 cut(s)	2522, 3338
ErhI	CCWWGG	5 cut(s)	1874, 2874, 3558, 4615, 4980
FalI	AAGNNNNNCTT	4 cut(s)	2164, 2196, 4431, 4463
FaqI	GGGAC	5 cut(s)	1611, 2164, 2485, 2856, 3783
FauNDI	CATATG	4 cut(s)	1040, 1048, 2617, 3075
FbaI	TGATCA	3 cut(s)	304, 2050, 3016
FriOI	GRGCYC	2 cut(s)	2522, 3338
FspAI	RTGCGCAY	1 cut(s)	1045
FspI	TGCGCA	1 cut(s)	1045
GlaI	GCGC	3 cut(s)	975, 1045, 2728
GsuI	CTGGAG	9 cut(s)	276, 475, 1091, 1791, 1875, 3182, 3480, 4544, 4597
HaeII	RGCGCY	2 cut(s)	977, 2730
HapII	CCGG	5 cut(s)	686, 1407, 2820, 4004, 4471
HgaI	GACGC	2 cut(s)	739, 2886
HhaI	GCGC	3 cut(s)	976, 1046, 2729
Hin1I	GRCGYC	1 cut(s)	2153
Hin6I	GCGC	3 cut(s)	974, 1044, 2727
HinP1I	GCGC	3 cut(s)	974, 1044, 2727
HincII	GTYRAC	6 cut(s)	852, 1822, 2743, 3115, 4014, 4570
HindII	GTYRAC	6 cut(s)	852, 1822, 2743, 3115, 4014, 4570
HindIII	AAGCTT	6 cut(s)	86, 882, 2013, 2346, 3833, 4253
HpaI	GTTAAC	1 cut(s)	852
HpaII	CCGG	5 cut(s)	686, 1407, 2820, 4004, 4471
HphI	GGTGA	6 cut(s)	675, 1178, 3024, 4220, 4772, 4874
Hpy99I	CGWCG	1 cut(s)	3393
HpyCH4IV	ACGT	6 cut(s)	2134, 2153, 2298, 2936, 3117, 4339
HpySE526I	ACGT	6 cut(s)	2134, 2153, 2298, 2936, 3117, 4339
Hsp92I	GRCGYC	1 cut(s)	2153
HspAI	GCGC	3 cut(s)	974, 1044, 2727
Ksp22I	TGATCA	3 cut(s)	304, 2050, 3016
KspAI	GTTAAC	1 cut(s)	852
LguI	GCTCTTC	1 cut(s)	792
MaeII	ACGT	6 cut(s)	2134, 2153, 2298, 2936, 3117, 4339
MfeI	CAATTG	5 cut(s)	237, 3642, 4302, 4325, 4851
MflI	RGATCY	6 cut(s)	1723, 2317, 3520, 3955, 4669, 4954
MhlI	GDGCHC	6 cut(s)	1103, 1695, 2522, 3338, 3558, 4177
MlsI	TGGCCA	3 cut(s)	330, 3207, 4807
MluNI	TGGCCA	3 cut(s)	330, 3207, 4807
MlyI	GAGTC	9 cut(s)	1472, 1805, 2288, 2878, 3173, 3938, 4126, 4355, 4664
MmeI	TCCRAC	8 cut(s)	132, 192, 1191, 2281, 3577, 3933, 4435, 4694
Mox20I	TGGCCA	3 cut(s)	330, 3207, 4807
Mph1103I	ATGCAT	2 cut(s)	544, 1774
MroXI	GAANNNNTTC	3 cut(s)	1266, 2605, 4443
MscI	TGGCCA	3 cut(s)	330, 3207, 4807
MslI	CAYNNNNRTG	3 cut(s)	1671, 3265, 4409
Msp20I	TGGCCA	3 cut(s)	330, 3207, 4807
MspA1I	CMGCKG	2 cut(s)	4031, 4435
MspI	CCGG	5 cut(s)	686, 1407, 2820, 4004, 4471
MspR9I	CCNGG	6 cut(s)	524, 764, 1854, 2961, 3498, 4005
MunI	CAATTG	5 cut(s)	237, 3642, 4302, 4325, 4851
Mva1269I	GAATGC	4 cut(s)	544, 590, 1081, 2160
MvaI	CCWGG	5 cut(s)	524, 764, 1854, 2961, 3498
MvnI	CGCG	1 cut(s)	617
NciI	CCSGG	1 cut(s)	4005
NcoI	CCATGG	2 cut(s)	4615, 4980
NdeI	CATATG	4 cut(s)	1040, 1048, 2617, 3075
NlaIV	GGNNCC	5 cut(s)	357, 2203, 3748, 3907, 4194
NmuCI	GTSAC	7 cut(s)	1673, 3165, 3617, 4118, 4208, 4306, 4358
NsbI	TGCGCA	1 cut(s)	1045
NsiI	ATGCAT	2 cut(s)	544, 1774
NspI	RCATGY	4 cut(s)	806, 3887, 4392, 4418
NspV	TTCGAA	1 cut(s)	2759
OliI	CACNNNNGTG	1 cut(s)	4409
PagI	TCATGA	1 cut(s)	504
PceI	AGGCCT	2 cut(s)	2216, 3860
PciI	ACATGT	1 cut(s)	4388
PciSI	GCTCTTC	1 cut(s)	792
PctI	GAATGC	4 cut(s)	544, 590, 1081, 2160
PdmI	GAANNNNTTC	3 cut(s)	1266, 2605, 4443
PflMI	CCANNNNNTGG	1 cut(s)	4736
PfoI	TCCNGGA	3 cut(s)	522, 2959, 4003
PinAI	ACCGGT	1 cut(s)	685
PleI	GAGTC	9 cut(s)	1471, 1805, 2287, 2877, 3172, 3938, 4125, 4355, 4663
PpsI	GAGTC	9 cut(s)	1471, 1805, 2287, 2877, 3172, 3938, 4125, 4355, 4663
Ppu21I	YACGTR	1 cut(s)	4340
PscI	ACATGT	1 cut(s)	4388
PshBI	ATTAAT	2 cut(s)	957, 3149
Psp124BI	GAGCTC	2 cut(s)	2522, 3338
Psp6I	CCWGG	5 cut(s)	522, 762, 1852, 2959, 3496
PspGI	CCWGG	5 cut(s)	522, 762, 1852, 2959, 3496
PspN4I	GGNNCC	5 cut(s)	357, 2203, 3748, 3907, 4194
PspPI	GGNCC	8 cut(s)	355, 929, 1964, 3661, 3746, 4193, 4612, 4739
PstI	CTGCAG	4 cut(s)	1795, 2122, 2386, 4993
PstNI	CAGNNNCTG	4 cut(s)	3915, 3976, 4034, 4157
PsuI	RGATCY	6 cut(s)	1723, 2317, 3520, 3955, 4669, 4954
PvuII	CAGCTG	2 cut(s)	4031, 4435
RseI	CAYNNNNRTG	3 cut(s)	1671, 3265, 4409
SacI	GAGCTC	2 cut(s)	2522, 3338
SapI	GCTCTTC	1 cut(s)	792
Sau96I	GGNCC	8 cut(s)	355, 929, 1964, 3661, 3746, 4193, 4612, 4739
ScaI	AGTACT	2 cut(s)	2924, 4704
SchI	GAGTC	9 cut(s)	1472, 1805, 2288, 2878, 3173, 3938, 4126, 4355, 4664
ScrFI	CCNGG	6 cut(s)	524, 764, 1854, 2961, 3498, 4005
SduI	GDGCHC	6 cut(s)	1103, 1695, 2522, 3338, 3558, 4177
SfcI	CTRYAG	7 cut(s)	774, 1791, 1956, 2118, 2382, 4112, 4989
SfuI	TTCGAA	1 cut(s)	2759
SinI	GGWCC	5 cut(s)	1964, 3746, 4193, 4612, 4739
SmiMI	CAYNNNNRTG	3 cut(s)	1671, 3265, 4409
SmlI	CTYRAG	4 cut(s)	89, 2951, 4056, 4930
SmoI	CTYRAG	4 cut(s)	89, 2951, 4056, 4930
SnaBI	TACGTA	1 cut(s)	4340
SpeI	ACTAGT	1 cut(s)	4408
SseBI	AGGCCT	2 cut(s)	2216, 3860
SsiI	CCGC	8 cut(s)	476, 711, 985, 1943, 2175, 2673, 2977, 4024
SspI	AATATT	2 cut(s)	2308, 4293
SstI	GAGCTC	2 cut(s)	2522, 3338
StuI	AGGCCT	2 cut(s)	2216, 3860
StyD4I	CCNGG	6 cut(s)	522, 762, 1852, 2959, 3496, 4003
StyI	CCWWGG	5 cut(s)	1874, 2874, 3558, 4615, 4980
TaiI	ACGT	6 cut(s)	2137, 2156, 2301, 2939, 3120, 4342
TatI	WGTACW	5 cut(s)	404, 426, 2922, 4391, 4702
TauI	GCSGC	1 cut(s)	1946
TseFI	GTSAC	7 cut(s)	1673, 3165, 3617, 4118, 4208, 4306, 4358
Tsp45I	GTSAC	7 cut(s)	1673, 3165, 3617, 4118, 4208, 4306, 4358
TspGWI	ACGGA	1 cut(s)	447
Van91I	CCANNNNNTGG	1 cut(s)	4736
VneI	GTGCAC	2 cut(s)	1099, 4173
VpaK11BI	GGWCC	5 cut(s)	1964, 3746, 4193, 4612, 4739
VspI	ATTAAT	2 cut(s)	957, 3149
XbaI	TCTAGA	1 cut(s)	5041
XceI	RCATGY	4 cut(s)	806, 3887, 4392, 4418
XcmI	CCANNNNNNNNNTGG	3 cut(s)	91, 115, 596
XmnI	GAANNNNTTC	3 cut(s)	1266, 2605, 4443
ZraI	GACGTC	1 cut(s)	2154
ZrmI	AGTACT	2 cut(s)	2924, 4704
Zsp2I	ATGCAT	2 cut(s)	544, 1774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.