Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0001909

Physical Location & Seq

Genomic Coordinates Forward (+)

45902725 .. 45911363

Gene Structure 8639 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG281300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5043 bp

ATGAACCATTTTTTGTTTGTTACAGAATCTCACTCACACATTATTGGAGGAAGTTCTACATATCTTTGGAAGATCAAAAGCTCCAAGCTTGAGCTGGAAATCGCCTCCAACTCCGATATGAGTCTCACTGCTCTTGACATTTCCCTTGAAGGATCACAATCTCCCCATGATATCAAGCATTTCGTCCCCAGAAGGTATCAAGTGAAAGTGTTCGAAGTCGCAAAGCGTAAGAACATAATTGCAGTGTTGGAAACTGGTTCTGGGAAAACTATGATTGCTGTGATGCTCATCAAACACATTGCTGATCAACACATCATCAAGTCCATTGGCCACAAGAAGTTGATAATTTTCATGGCCCCAACAGTTCATCTTGTCATTCAGCAATATGAGGTTATAAAAAAGTCTACAACTCTTGAAGTGGGAGTATACTACGGAGCTAAGGGCGTTGATGACTGGAGTCCAGAATATTGGGAGGTGGAAGTGAATACACATAATATAATGGTCATGACACCTCAAATTCTCCTGGATGCTTTGCGGAATGCATTTTTGAAGATAGAGATGATTTGCTTGATGGTCTTTGATGAATGCCACCGTGCCACTGGCAATCATCCCTACACGAAAATAATGAAGGAATTTCATCACAAATCAGGAGACAAGCCAAAGATTTTTGGAATGACAGCATCACCAGTCATTAAAAAAGGTGTCTCATCCGCCACGGACTGTGAGGATCAGATATCACAACTTGAAAGCATCTTGGATTCCCAGGTTTATACAGTAGAAGACAGGACAGAGATTGAAGAGCATGTTCCCTCAGCAGTCCAAATTTGTAAATTTTATGACCCGACATGGTTGATCTCTGTGGATTTGAAAGCAAAAATGGAAGCTTTATGGTCAAAGTTTGATGCTTCTATCATGTCAAAGTTGCAAGGGCCAGAGAAAAGTGAGTACGAAGACATTAATGATAAAATCAAGATGCTACGAAAGCGGTTGTCCAATGACTACATGAAAATTTTATATTGCCTTGATGATCTTGGTATCGTATGCGCATATGAGGCTGTGAAAGTCTGTCTGGAGAATGCCCCTGATGTCAAAGAGGAGTGCACATTTTATAGAGAAAGCTCTTTGCAATGCAGATATTTTCTTGATGAAGTCTTAAATATGATCAGTGAATTTCTTCAAAAAGGTTATATACTTCATACAGATCTTGAGTCAGACTATATAAAGGCACAAAAAGTGGGATACATATCTCCGAAGTTACATGAACTTCTTCAAATTTTCCACTCATTTGAGAGGGATAGGCAAGTTCTGTGCCTTATTTTCGTAGAGAGAATTGTCACAGCCAAAGTAATGCAAAGATATGTGAAGAAACTGCCATGTTTATCTAATTTCACAGCTTCATATTTGACCGGAAGTACCACATCAGTTGATGCACTTGCACCGAAATTGCAGAAGGAAATTTTGGAGTCATTTTACAGTGGGAAGGTCAATCTACTATTTGCCACTGATGTAGTTGAAGAGGGAATTCATGTCCAGAACTGCTCCTGTGTGATCCGGTTTGATCTTCCCAAGACAGTTCGTAGCTATGTCCAGTCTCGGGGACGAGCACGAAAAAATGATTCCCAATATATCATAATGCTTGAAAGGGACAACAAGAAGCAAAGAGACCATCTCTTTGACATAATCAGGAGTGAGTACTCAATGACAGATACGTCGAGAAATCGAGATCCTGATGGATGCAATTTAAGAGCTTGTAACTTTGAAGATGCAAATGCATACTATGTAGAAGCTACTGGAGCATCAATTACTCCAGACTCTAGTGTCAGCCTCATATATCGGTACTGTGAAAAGCTCCCTGGAGATAGGTACTTTATTCCAAGGCCAACTTTTCATTTCAGACTATTTGAAGGGTTGCATCAGTGTGAAATAACATTACCTCCAAATGCGGCATTTCAAACACTAGTTGGTCCTATGAGTAGAAACGGTCATTCATCAAAGCAGCTTGTATGCTTGGAAGCTTGTAAAATCCTGCATCAAATGGGTGCTTTGGATGATCATCTTCTCCCACTTGTCGAAGTGCCTCTTGATAATGAAGTGATTGTGAAAAGCAAAGAATTATCTGCAGGTGCTGGAACTACAAAAAGGAAAGAGTTACATGGGATGACTTGCATTCGTGCATTAAGTGGAACTTGGGGAGAGAAAGTTGATGGTGCTAGTTTTGAGGCCTACAAATTTGAGTTCTCCTGTAATGTTGATGAGACATATTCAGGATTCGTACTACTAGTCGAGATGAAACTTGATGATGACGTGGGAAATACTGAAGTGGATCTCTACTTAATTGCAAAGATGGTCAAAGCTTACGTTTCTTCATGTGGAAAGGTGCATCTCAGTGCAGATCAGATAAAGGAAGCCATGTGCTTTCAGGAATTTTTCTTTAATGGTTTGTTTGGGAGATTATTTCTTGGATCCGAAGCAAAAGGGACAGAAAGAAAATTTCTACTTCAGACAGAAATAAAATCACTATGGTGCTCATCGTACAGCTATTTACTTTTACCGACTGAGGCATCTGACAGTTCAAATAAGAAATCTTGGAACATAGATTGGATGGGAATCTGTTCCTGTGCTAATGTGGTGGAGTTTCTTAGGAAACACTATTCCAATGGTCTTGAGAATTTCAAAGGCGGTAGAGGAAATATATTACTTTCTAGGACTGGTTCATCCATGACAGATTGCAAGGCTGAAAATTTAGTCCAGTTTGCTAATAGTTCAATTGATGTCAATGATCTTGGAGATATGGTAGTGTTGGCTATTCACACAGGAAGAATCTACTCTGTTATTGAAGTTGTTAGTAGCATTTCTGCTGAGAGTCCCTTGGAAGAAAATGCTGATACTGCCTCATCAGATTATACTTATGTTGAGTACTTCAAAACAACGTATGGGATTGTACTCAAGTATCCTAGACATCCTCTATTGCGGTTGAAGCAAAGTCATAATGCACATAACCTGCTTGTTGATCAAGGTGTGTCATCACCAGATGGTCTAGTTCAAGAAAAGCAACAAGCTCATGTTCATATGCCACCCGAAATCTTAGTCAGTATTGATGTTCGAGTTGACGTTCTAAAATCATTTTATTTACTACCATCACTAATGTGTCGTCTGGAGTCCCTAATGTTAGCTAGCCAGATCAGGAAAGAGATCAATGGCCAGTCTAGCAACTTTTATATATCAAGCTCATTGATTCTAGAAGCACTCACAACACTTAGATGCTGTGAAAATTTTTCATTGGAGCGTTTGGAGTTGCTTGGAGATTCAGTTCTCAAATATGCTGTGAGCTCCTACCTCTTTCTTAAATATCCCGAGAAACATGAAGGACAATTAACTGCTCGACGAACAAGAGCTATTTGCAACTCAAACCTGCATAAACTAGGAACGAAAAACAAGTTACAGGGATACATACGAGACGGTGCATTTGAACCACGACGTTGGGCTGCTCCAGGGCAGCTTTCTAGATTCCCAGATCCATGTCAATGCGGAGTGGATACTTTGGAAGTGCCCTTGGACAGTAGATTTCAAACTGAAAAGGCTGTTAAGGTTGGAAAATTCTGTGATAAAGGTCACAGATGGATGAACTCAAAAACCATTGCTGATTGTGTTGAGGCCCTCATAGGAGCTTATTATGTTGGTGGAGGATTGTTTGCTGCACTTCATTTGATGAAGTGGCTCAGCATTGATTCTGAAACTGGACCCTCATTTGCTGATGAAGCCATTACAAGGGCATCACTTCGATCCTACAATCCCAAAACTAATGAACTTGCTAACTTGGAGTTGAAGCTTAGCTATGAATTTGCAGTCAAGGGCTTGTTACATGAGGCCATCACACATGCTTCTCAGCAAAAACTAGGTGCCAGCTACTGTTATCAGAGGCTTGAATTTCTTGGAGACTCTGTGTTGGATCTGCTAACTACACAGCATCTCTATCGCAGCCACACAGATATTGATCCTGGAGTGTGGACTGATTTGCGGTCAGCTGCTGTTAGTAATGAAAACTTTGCTCAAGCTGCTATTAGACAAAACCTTCATCTACATCTTCAACATTGCTCTGGGTTACTTCGGAGTCACATAACCGAGTATGAGAGGTTGCTTACAGAAGCTGAGCACAGTACCAGCTCAGTGGAAGACATTAAGTGTCCAAAGGCTCTTGGTGACATGGTGGAAAGTATAGCAGGAGCTATACTAATTGATACAAAGCTTAATCTTGATGAAGTATGGAGGGTTTTTAAACCAATATTGTCTCCAATTGTGACCCCTTCTAGTCTTCAATTGCATCCGCTACGTAAACTGTCTGAATTATGTGATTCACTTGGGTACTTTGTGAAAGAAACATGTACTAAAGATGATACACTAGTGAATGTGGAACTAAGTTTGCAGCTGGAAGATGTTCTTTTGGTTGGAAACGGCTTTGACCGGAGTAGAAAAGCTGCGAAACATAAGGCAGCTCGTCAAGTGTTGAAGGCGCTCGAGAAAAGCATAGAAAGGAGAGAGCATTATTCTGATATGGATAGTGACCACTGCAGCAACCTAACTGATGAAGATTTTTCAGAGCCAATGAACCATGGAAAGCAGAATATGACAACAATTCAATCATCTTCAGAGTCAAAAACAATAGATCCTACCCAATCCAATGACTGTTCTAATGAAGTCTTAAGTCACAACATTGCAACCCCAGTTATTGGTCCAATCGATACGAAAAAAGGTGAACCTCGAAAATCTCTCTTTGATCTATGTAAGAAATTGCAGTGGCCAATGCCTGCATTCACAACAACAGAGCAGAAGTCAAGAACTCCAATTGAATTCGGTGAGGGTTCTGAAAAGAGAATAGGTTTCAGCAGCTTTCAATCAAAAATCAGCTTGCACATACCCAACTCTTGCACTATTGTATGCACAGGAGATCCTAGAGCCGATAAGAAGACTTCCATGGATTCTGCAGTTGTTTCCATGCTTCACGAGCTTCAACGACGGAGAAAACTTGTCATTCTCCAGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1680

Amino Acids

189.6

Weight (kDa)

6.32

Isoelectric Point (pI)

43.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	63 - 229	1.4e-16	Type III restriction enzyme, res subunit
DEAD	PF00270	65 - 229	1.7e-18	DEAD/DEAH box helicase
Helicase_C	PF00271	418 - 535	1.9e-17	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	606 - 690	2.9e-22	Dicer dimerisation domain
PAZ	PF02170	925 - 1030	2.3e-09	PAZ domain
Ribonuclease_3	PF00636	1095 - 1228	6.7e-21	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1095 - 1152	2.5e-07	Ribonuclease-III-like
Ribonucleas_3_3	PF14622	1285 - 1413	6.7e-13	Ribonuclease-III-like
Ribonuclease_3	PF00636	1306 - 1414	8.1e-18	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001909)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920
fragaria_vesca	FvH4_2g07790 FvH4_2g18930 FvH4_2g18930 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780
malus_domestica	MD08G1046300.v1.1 MD10G1163700.v1.1 MD15G1047500.v1.1
prunus_persica	Prupe.1G401900_v2.0.a1 Prupe.1G401900_v2.0.a1 Prupe.8G202000_v2.0.a1 Prupe.8G202000_v2.0.a1
pyrus_communis	pycom08g03830 pycom08g03840 pycom15g04670
rosa_chinensis	RchiOBHm_Chr6g0284721 RchiOBHm_Chr6g0312121
rosa_laevigata	RLG00000010353 RLG00000012724
rosa_multiflora	Rmu_sc0004065.1_g000007 Rmu_sc0007474.1_g000021
rosa_roxburghii	Rroxscaffold_7G00157050 Rroxscaffold_7G00184440
rosa_rugosa	Rorug06G0165900 Rorug06G0392600 Rorug06G0392700 Rorug06G0392800
rosa_samantha	Rh4CG118000 Rh6AG279000 Rh6AG509700 Rh6BG279000 Rh6BG520600 Rh6CG281300 Rh6CG526600 Rh6DG274000 Rh6DG512000
rosa_wichuraiana	Rw6G023980 Rw6G044350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	395
AarI	CACCTGC	1 cut(s)	2111
AasI	GACNNNNNNGTC	2 cut(s)	1708, 1817
Acc16I	TGCGCA	1 cut(s)	1045
Acc36I	ACCTGC	3 cut(s)	2111, 3009, 3420
AccB1I	GGYRCC	1 cut(s)	3899
AccB7I	CCANNNNNTGG	1 cut(s)	4727
AccI	GTMKAC	2 cut(s)	404, 426
AciI	CCGC	9 cut(s)	535, 711, 985, 1943, 2679, 2971, 3528, 4018, 4325
AcoI	YGGCCR	3 cut(s)	328, 3199, 4796
AcuI	CTGAAG	3 cut(s)	2337, 2483, 4629
AfiI	CCNNNNNNNGG	3 cut(s)	1594, 3768, 4727
AflII	CTTAAG	1 cut(s)	4699
AflIII	ACRYGT	1 cut(s)	4379
AhlI	ACTAGT	3 cut(s)	1957, 2278, 4399
AjiI	CACGTC	1 cut(s)	2305
AjnI	CCWGG	5 cut(s)	522, 762, 1852, 3490, 3997
AjuI	GAANNNNNNNTTGG	2 cut(s)	1442, 1474
AleI	CACNNNNGTG	2 cut(s)	2521, 3145
AloI	GAACNNNNNNTCC	2 cut(s)	3294, 3326
Alw21I	GWGCWC	5 cut(s)	1103, 1606, 2528, 3332, 4155
Alw26I	GTCTC	9 cut(s)	128, 645, 709, 1596, 1656, 2249, 3450, 3930, 4293
Alw44I	GTGCAC	1 cut(s)	1099
AlwNI	CAGNNNCTG	4 cut(s)	2126, 3909, 4028, 4148
Ama87I	CYCGRG	3 cut(s)	1593, 3353, 4514
AoxI	GGCC	9 cut(s)	328, 354, 929, 1877, 2220, 3199, 3654, 3867, 4796
ApaLI	GTGCAC	1 cut(s)	1099
ArsI	GACNNNNNNTTYG	6 cut(s)	1655, 1687, 3072, 3104, 3449, 3481
AseI	ATTAAT	1 cut(s)	957
Asp700I	GAANNNNTTC	5 cut(s)	209, 1173, 1266, 2611, 4434
AspLEI	GCGC	2 cut(s)	1046, 4513
AspS9I	GGNCC	6 cut(s)	355, 929, 1964, 3655, 3740, 4730
AsuHPI	GGTGA	5 cut(s)	675, 3018, 4211, 4763, 4865
AsuII	TTCGAA	1 cut(s)	213
AsuNHI	GCTAGC	1 cut(s)	3173
AvaI	CYCGRG	3 cut(s)	1593, 3353, 4514
AvaII	GGWCC	3 cut(s)	1964, 3740, 4730
BaeGI	GKGCMC	2 cut(s)	1103, 3552
BaeI	ACNNNNGTAYC	4 cut(s)	2933, 2966, 4386, 4419
BalI	TGGCCA	3 cut(s)	330, 3201, 4798
BamHI	GGATCC	1 cut(s)	2462
BanI	GGYRCC	1 cut(s)	3899
BanII	GRGCYC	1 cut(s)	3332
BauI	CACGAG	1 cut(s)	5000
BbsI	GAAGAC	5 cut(s)	786, 957, 4179, 4304, 4970
Bbv12I	GWGCWC	5 cut(s)	1103, 1606, 2528, 3332, 4155
BbvCI	CCTCAGC	1 cut(s)	811
BceAI	ACGGC	1 cut(s)	4468
BcgI	CGANNNNNNTGC	2 cut(s)	3956, 3990
BciT130I	CCWGG	5 cut(s)	524, 764, 1854, 3492, 3999
BciVI	GTATCC	4 cut(s)	1232, 2961, 3440, 3529
BclI	TGATCA	4 cut(s)	304, 1161, 2050, 3010
BcoDI	GTCTC	9 cut(s)	128, 645, 709, 1596, 1656, 2249, 3450, 3930, 4293
BcuI	ACTAGT	3 cut(s)	1957, 2278, 4399
BfmI	CTRYAG	3 cut(s)	2118, 4567, 4980
BfoI	RGCGCY	1 cut(s)	4514
BfrI	CTTAAG	1 cut(s)	4699
BfuAI	ACCTGC	3 cut(s)	2111, 3009, 3420
BfuI	GTATCC	4 cut(s)	1232, 2961, 3440, 3529
BglII	AGATCT	1 cut(s)	1201
BlpI	GCTNAGC	3 cut(s)	3719, 3830, 4149
BmcAI	AGTACT	2 cut(s)	1694, 2918
Bme1390I	CCNGG	5 cut(s)	524, 764, 1854, 3492, 3999
Bme18I	GGWCC	3 cut(s)	1964, 3740, 4730
BmeT110I	CYCGRG	3 cut(s)	1593, 3353, 4514
BmgBI	CACGTC	1 cut(s)	2305
BmgT120I	GGNCC	6 cut(s)	355, 929, 1964, 3655, 3740, 4730
BmiI	GGNNCC	4 cut(s)	357, 2464, 3742, 3901
BmrFI	CCNGG	5 cut(s)	524, 764, 1854, 3492, 3999
BmrI	ACTGGG	1 cut(s)	4715
BmtI	GCTAGC	1 cut(s)	3177
BmuI	ACTGGG	1 cut(s)	4715
BoxI	GACNNNNGTC	1 cut(s)	456
BpiI	GAAGAC	5 cut(s)	786, 957, 4179, 4304, 4970
BplI	GAGNNNNNCTC	2 cut(s)	1653, 1685
BpmI	CTGGAG	9 cut(s)	475, 1091, 1791, 1812, 1875, 3176, 3474, 4020, 5018
Bpu10I	CCTNAGC	2 cut(s)	438, 811
Bpu1102I	GCTNAGC	3 cut(s)	3719, 3830, 4149
Bpu14I	TTCGAA	1 cut(s)	213
BpuEI	CTTGAG	5 cut(s)	110, 1226, 2684, 2930, 4035
Bsa29I	ATCGAT	1 cut(s)	4737
BsaAI	YACGTR	1 cut(s)	4331
BsaBI	GATNNNNATC	6 cut(s)	157, 287, 1243, 2052, 2606, 3993
BsaI	GGTCTC	1 cut(s)	1656
BsaJI	CCNNGG	9 cut(s)	714, 762, 1852, 1874, 2868, 3491, 3552, 4609, 4971
BsaWI	WCCGGW	3 cut(s)	1406, 1551, 4461
Bsc4I	CCNNNNNNNGG	3 cut(s)	1594, 3768, 4727
Bse3DI	GCAATG	5 cut(s)	297, 1133, 3636, 4090, 4710
Bse8I	GATNNNNATC	6 cut(s)	157, 287, 1243, 2052, 2606, 3993
BseBI	CCWGG	5 cut(s)	524, 764, 1854, 3492, 3999
BseCI	ATCGAT	1 cut(s)	4737
BseDI	CCNNGG	9 cut(s)	714, 762, 1852, 1874, 2868, 3491, 3552, 4609, 4971
BseJI	GATNNNNATC	6 cut(s)	157, 287, 1243, 2052, 2606, 3993
BseLI	CCNNNNNNNGG	3 cut(s)	1594, 3768, 4727
BseMI	GCAATG	5 cut(s)	297, 1133, 3636, 4090, 4710
BseMII	CTCAG	8 cut(s)	825, 2398, 2547, 2850, 3733, 3899, 4140, 4179
BseRI	GAGGAG	1 cut(s)	1109
BseSI	GKGCMC	2 cut(s)	1103, 3552
BsgI	GTGCAG	2 cut(s)	2409, 3681
BshFI	GGCC	9 cut(s)	330, 356, 931, 1879, 2222, 3201, 3656, 3869, 4798
BshNI	GGYRCC	1 cut(s)	3899
BshVI	ATCGAT	1 cut(s)	4737
BsiHKAI	GWGCWC	5 cut(s)	1103, 1606, 2528, 3332, 4155
BsiHKCI	CYCGRG	3 cut(s)	1593, 3353, 4514
BsiSI	CCGG	3 cut(s)	1407, 1552, 4462
BslFI	GGGAC	6 cut(s)	170, 1611, 1658, 2491, 2850, 3145
BslI	CCNNNNNNNGG	3 cut(s)	1594, 3768, 4727
BsmAI	GTCTC	9 cut(s)	128, 645, 709, 1596, 1656, 2249, 3450, 3930, 4293
BsmBI	CGTCTC	1 cut(s)	3450
BsmFI	GGGAC	6 cut(s)	170, 1611, 1658, 2491, 2850, 3145
BsmI	GAATGC	5 cut(s)	544, 590, 1081, 2166, 4808
BsnI	GGCC	9 cut(s)	330, 356, 931, 1879, 2222, 3201, 3656, 3869, 4798
Bso31I	GGTCTC	1 cut(s)	1656
BsoBI	CYCGRG	3 cut(s)	1593, 3353, 4514
Bsp119I	TTCGAA	1 cut(s)	213
Bsp1286I	GDGCHC	6 cut(s)	1103, 1606, 2528, 3332, 3552, 4155
Bsp1720I	GCTNAGC	3 cut(s)	3719, 3830, 4149
Bsp19I	CCATGG	2 cut(s)	4609, 4971
BspACI	CCGC	9 cut(s)	535, 711, 985, 1943, 2679, 2971, 3528, 4018, 4325
BspANI	GGCC	9 cut(s)	330, 356, 931, 1879, 2222, 3201, 3656, 3869, 4798
BspCNI	CTCAG	8 cut(s)	824, 2397, 2548, 2851, 3732, 3898, 4141, 4178
BspDI	ATCGAT	1 cut(s)	4737
BspHI	TCATGA	1 cut(s)	504
BspLI	GGNNCC	4 cut(s)	357, 2464, 3742, 3901
BspMAI	CTGCAG	3 cut(s)	2122, 4571, 4984
BspMI	ACCTGC	3 cut(s)	2111, 3009, 3420
BspOI	GCTAGC	1 cut(s)	3177
BspQI	GCTCTTC	1 cut(s)	792
BspT104I	TTCGAA	1 cut(s)	213
BspT107I	GGYRCC	1 cut(s)	3899
BspTI	CTTAAG	1 cut(s)	4699
BspTNI	GGTCTC	1 cut(s)	1656
BsrDI	GCAATG	5 cut(s)	297, 1133, 3636, 4090, 4710
BssECI	CCNNGG	9 cut(s)	714, 762, 1852, 1874, 2868, 3491, 3552, 4609, 4971
BssNAI	GTATAC	1 cut(s)	427
BssSI	CACGAG	1 cut(s)	5000
BssT1I	CCWWGG	5 cut(s)	1874, 2868, 3552, 4609, 4971
Bst1107I	GTATAC	1 cut(s)	427
Bst2BI	CACGAG	1 cut(s)	5000
Bst2UI	CCWGG	5 cut(s)	524, 764, 1854, 3492, 3999
Bst6I	CTCTTC	2 cut(s)	792, 1509
BstAFI	CTTAAG	1 cut(s)	4699
BstBAI	YACGTR	1 cut(s)	4331
BstBI	TTCGAA	1 cut(s)	213
BstC8I	GCNNGC	4 cut(s)	3175, 3904, 4806, 4907
BstDSI	CCRYGG	3 cut(s)	714, 4609, 4971
BstH2I	RGCGCY	1 cut(s)	4514
BstHHI	GCGC	2 cut(s)	1046, 4513
BstMAI	GTCTC	9 cut(s)	128, 645, 709, 1596, 1656, 2249, 3450, 3930, 4293
BstMWI	GCNNNNNNNGC	9 cut(s)	982, 1052, 1793, 2888, 2977, 3207, 3758, 4055, 5002
BstNI	CCWGG	5 cut(s)	524, 764, 1854, 3492, 3999
BstNSI	RCATGY	3 cut(s)	806, 3881, 4383
BstPAI	GACNNNNGTC	1 cut(s)	456
BstSCI	CCNGG	5 cut(s)	522, 762, 1852, 3490, 3997
BstSFI	CTRYAG	3 cut(s)	2118, 4567, 4980
BstSLI	GKGCMC	2 cut(s)	1103, 3552
BstSNI	TACGTA	1 cut(s)	4331
BstV2I	GAAGAC	5 cut(s)	786, 957, 4179, 4304, 4970
BstX2I	RGATCY	8 cut(s)	1201, 1723, 2323, 2462, 3514, 3949, 4663, 4945
BstXI	CCANNNNNNTGG	3 cut(s)	468, 3480, 4168
BstYI	RGATCY	8 cut(s)	1201, 1723, 2323, 2462, 3514, 3949, 4663, 4945
BstZ17I	GTATAC	1 cut(s)	427
Bsu15I	ATCGAT	1 cut(s)	4737
BsuI	GTATCC	4 cut(s)	1232, 2961, 3440, 3529
BsuRI	GGCC	9 cut(s)	330, 356, 931, 1879, 2222, 3201, 3656, 3869, 4798
BsuTUI	ATCGAT	1 cut(s)	4737
BtgI	CCRYGG	3 cut(s)	714, 4609, 4971
BtrI	CACGTC	1 cut(s)	2305
BtsI	GCAGTG	4 cut(s)	126, 249, 4564, 4799
BveI	ACCTGC	3 cut(s)	2111, 3009, 3420
Cac8I	GCNNGC	4 cut(s)	3175, 3904, 4806, 4907
CaiI	CAGNNNCTG	4 cut(s)	2126, 3909, 4028, 4148
CciI	TCATGA	1 cut(s)	504
CfoI	GCGC	2 cut(s)	1046, 4513
Cfr13I	GGNCC	6 cut(s)	355, 929, 1964, 3655, 3740, 4730
ClaI	ATCGAT	1 cut(s)	4737
CspCI	CAANNNNNGTGG	2 cut(s)	1405, 1440
DraI	TTTAAA	1 cut(s)	4276
DrdI	GACNNNNNNGTC	2 cut(s)	1708, 1817
DseDI	GACNNNNNNGTC	2 cut(s)	1708, 1817
EaeI	YGGCCR	3 cut(s)	328, 3199, 4796
Eam1104I	CTCTTC	2 cut(s)	792, 1509
EarI	CTCTTC	2 cut(s)	792, 1509
EciI	GGCGGA	1 cut(s)	700
Ecl136II	GAGCTC	1 cut(s)	3330
Eco105I	TACGTA	1 cut(s)	4331
Eco130I	CCWWGG	5 cut(s)	1874, 2868, 3552, 4609, 4971
Eco147I	AGGCCT	1 cut(s)	2222
Eco24I	GRGCYC	1 cut(s)	3332
Eco31I	GGTCTC	1 cut(s)	1656
Eco32I	GATATC	2 cut(s)	172, 735
Eco47I	GGWCC	3 cut(s)	1964, 3740, 4730
Eco53kI	GAGCTC	1 cut(s)	3330
Eco57I	CTGAAG	3 cut(s)	2337, 2483, 4629
Eco88I	CYCGRG	3 cut(s)	1593, 3353, 4514
EcoICRI	GAGCTC	1 cut(s)	3330
EcoO109I	RGGNCCY	1 cut(s)	3655
EcoRI	GAATTC	2 cut(s)	1521, 4847
EcoRII	CCWGG	5 cut(s)	522, 762, 1852, 3490, 3997
EcoRV	GATATC	2 cut(s)	172, 735
EcoT14I	CCWWGG	5 cut(s)	1874, 2868, 3552, 4609, 4971
EcoT22I	ATGCAT	2 cut(s)	544, 1774
EcoT38I	GRGCYC	1 cut(s)	3332
ErhI	CCWWGG	5 cut(s)	1874, 2868, 3552, 4609, 4971
Esp3I	CGTCTC	1 cut(s)	3450
FalI	AAGNNNNNCTT	8 cut(s)	1867, 1899, 2064, 2096, 2170, 2202, 4422, 4454
FaqI	GGGAC	6 cut(s)	170, 1611, 1658, 2491, 2850, 3145
FauNDI	CATATG	2 cut(s)	1048, 3069
FbaI	TGATCA	4 cut(s)	304, 1161, 2050, 3010
FblI	GTMKAC	2 cut(s)	404, 426
FriOI	GRGCYC	1 cut(s)	3332
FspAI	RTGCGCAY	1 cut(s)	1045
FspI	TGCGCA	1 cut(s)	1045
GlaI	GCGC	2 cut(s)	1045, 4512
GsuI	CTGGAG	9 cut(s)	475, 1091, 1791, 1812, 1875, 3176, 3474, 4020, 5018
HaeII	RGCGCY	1 cut(s)	4514
HaeIII	GGCC	9 cut(s)	330, 356, 931, 1879, 2222, 3201, 3656, 3869, 4798
HapII	CCGG	3 cut(s)	1407, 1552, 4462
HhaI	GCGC	2 cut(s)	1046, 4513
Hin6I	GCGC	2 cut(s)	1044, 4511
HinP1I	GCGC	2 cut(s)	1044, 4511
HincII	GTYRAC	1 cut(s)	3109
HindII	GTYRAC	1 cut(s)	3109
HindIII	AAGCTT	6 cut(s)	86, 882, 2013, 2352, 3827, 4244
HpaII	CCGG	3 cut(s)	1407, 1552, 4462
HphI	GGTGA	5 cut(s)	675, 3018, 4211, 4763, 4865
Hpy166II	GTNNAC	7 cut(s)	405, 427, 1101, 3109, 4008, 4334, 4754
Hpy8I	GTNNAC	7 cut(s)	405, 427, 1101, 3109, 4008, 4334, 4754
Hpy99I	CGWCG	4 cut(s)	1714, 3387, 3480, 5016
HpyCH4IV	ACGT	7 cut(s)	1709, 2304, 2358, 2930, 3111, 3478, 4330
HpyF10VI	GCNNNNNNNGC	9 cut(s)	982, 1052, 1793, 2888, 2977, 3207, 3758, 4055, 5002
HpySE526I	ACGT	7 cut(s)	1709, 2304, 2358, 2930, 3111, 3478, 4330
HspAI	GCGC	2 cut(s)	1044, 4511
Ksp22I	TGATCA	4 cut(s)	304, 1161, 2050, 3010
LguI	GCTCTTC	1 cut(s)	792
MaeII	ACGT	7 cut(s)	1709, 2304, 2358, 2930, 3111, 3478, 4330
MfeI	CAATTG	4 cut(s)	2766, 4293, 4316, 4842
MflI	RGATCY	8 cut(s)	1201, 1723, 2323, 2462, 3514, 3949, 4663, 4945
MhlI	GDGCHC	6 cut(s)	1103, 1606, 2528, 3332, 3552, 4155
MlsI	TGGCCA	3 cut(s)	330, 3201, 4798
MluNI	TGGCCA	3 cut(s)	330, 3201, 4798
MmeI	TCCRAC	5 cut(s)	132, 228, 3571, 3927, 4426
Mox20I	TGGCCA	3 cut(s)	330, 3201, 4798
Mph1103I	ATGCAT	2 cut(s)	544, 1774
MroXI	GAANNNNTTC	5 cut(s)	209, 1173, 1266, 2611, 4434
MscI	TGGCCA	3 cut(s)	330, 3201, 4798
MslI	CAYNNNNRTG	6 cut(s)	1917, 2385, 2521, 3145, 3259, 3523
Msp20I	TGGCCA	3 cut(s)	330, 3201, 4798
MspA1I	CMGCKG	2 cut(s)	4025, 4426
MspCI	CTTAAG	1 cut(s)	4699
MspI	CCGG	3 cut(s)	1407, 1552, 4462
MspR9I	CCNGG	5 cut(s)	524, 764, 1854, 3492, 3999
MunI	CAATTG	4 cut(s)	2766, 4293, 4316, 4842
Mva1269I	GAATGC	5 cut(s)	544, 590, 1081, 2166, 4808
MvaI	CCWGG	5 cut(s)	524, 764, 1854, 3492, 3999
MwoI	GCNNNNNNNGC	9 cut(s)	982, 1052, 1793, 2888, 2977, 3207, 3758, 4055, 5002
NcoI	CCATGG	2 cut(s)	4609, 4971
NdeI	CATATG	2 cut(s)	1048, 3069
NheI	GCTAGC	1 cut(s)	3173
NlaIV	GGNNCC	4 cut(s)	357, 2464, 3742, 3901
NmuCI	GTSAC	7 cut(s)	1333, 3611, 4112, 4199, 4297, 4559, 4703
NsbI	TGCGCA	1 cut(s)	1045
NsiI	ATGCAT	2 cut(s)	544, 1774
NspI	RCATGY	3 cut(s)	806, 3881, 4383
NspV	TTCGAA	1 cut(s)	213
OliI	CACNNNNGTG	2 cut(s)	2521, 3145
PaeR7I	CTCGAG	1 cut(s)	4514
PagI	TCATGA	1 cut(s)	504
PaqCI	CACCTGC	1 cut(s)	2111
PceI	AGGCCT	1 cut(s)	2222
PciI	ACATGT	1 cut(s)	4379
PciSI	GCTCTTC	1 cut(s)	792
PctI	GAATGC	5 cut(s)	544, 590, 1081, 2166, 4808
PdmI	GAANNNNTTC	5 cut(s)	209, 1173, 1266, 2611, 4434
PflMI	CCANNNNNTGG	1 cut(s)	4727
PfoI	TCCNGGA	2 cut(s)	522, 3997
Ppu21I	YACGTR	1 cut(s)	4331
PscI	ACATGT	1 cut(s)	4379
PshAI	GACNNNNGTC	1 cut(s)	456
PshBI	ATTAAT	1 cut(s)	957
PsiI	TTATAA	1 cut(s)	395
Psp124BI	GAGCTC	1 cut(s)	3332
Psp6I	CCWGG	5 cut(s)	522, 762, 1852, 3490, 3997
PspGI	CCWGG	5 cut(s)	522, 762, 1852, 3490, 3997
PspN4I	GGNNCC	4 cut(s)	357, 2464, 3742, 3901
PspPI	GGNCC	6 cut(s)	355, 929, 1964, 3655, 3740, 4730
PstI	CTGCAG	3 cut(s)	2122, 4571, 4984
PstNI	CAGNNNCTG	4 cut(s)	2126, 3909, 4028, 4148
PsuI	RGATCY	8 cut(s)	1201, 1723, 2323, 2462, 3514, 3949, 4663, 4945
PvuII	CAGCTG	2 cut(s)	4025, 4426
RseI	CAYNNNNRTG	6 cut(s)	1917, 2385, 2521, 3145, 3259, 3523
SacI	GAGCTC	1 cut(s)	3332
SapI	GCTCTTC	1 cut(s)	792
Sau96I	GGNCC	6 cut(s)	355, 929, 1964, 3655, 3740, 4730
ScaI	AGTACT	2 cut(s)	1694, 2918
ScrFI	CCNGG	5 cut(s)	524, 764, 1854, 3492, 3999
SduI	GDGCHC	6 cut(s)	1103, 1606, 2528, 3332, 3552, 4155
SfcI	CTRYAG	3 cut(s)	2118, 4567, 4980
Sfr274I	CTCGAG	1 cut(s)	4514
SfuI	TTCGAA	1 cut(s)	213
SinI	GGWCC	3 cut(s)	1964, 3740, 4730
SlaI	CTCGAG	1 cut(s)	4514
SmiMI	CAYNNNNRTG	6 cut(s)	1917, 2385, 2521, 3145, 3259, 3523
SmlI	CTYRAG	7 cut(s)	89, 1205, 2663, 2945, 4050, 4514, 4699
SmoI	CTYRAG	7 cut(s)	89, 1205, 2663, 2945, 4050, 4514, 4699
SnaBI	TACGTA	1 cut(s)	4331
SpeI	ACTAGT	3 cut(s)	1957, 2278, 4399
SseBI	AGGCCT	1 cut(s)	2222
SsiI	CCGC	9 cut(s)	535, 711, 985, 1943, 2679, 2971, 3528, 4018, 4325
SspI	AATATT	2 cut(s)	467, 4284
SstI	GAGCTC	1 cut(s)	3332
StuI	AGGCCT	1 cut(s)	2222
StyD4I	CCNGG	5 cut(s)	522, 762, 1852, 3490, 3997
StyI	CCWWGG	5 cut(s)	1874, 2868, 3552, 4609, 4971
TaiI	ACGT	7 cut(s)	1712, 2307, 2361, 2933, 3114, 3481, 4333
TatI	WGTACW	4 cut(s)	1692, 2916, 2941, 4382
TauI	GCSGC	1 cut(s)	1946
TseFI	GTSAC	7 cut(s)	1333, 3611, 4112, 4199, 4297, 4559, 4703
Tsp45I	GTSAC	7 cut(s)	1333, 3611, 4112, 4199, 4297, 4559, 4703
TspGWI	ACGGA	3 cut(s)	447, 731, 5029
Van91I	CCANNNNNTGG	1 cut(s)	4727
Vha464I	CTTAAG	1 cut(s)	4699
VneI	GTGCAC	1 cut(s)	1099
VpaK11BI	GGWCC	3 cut(s)	1964, 3740, 4730
VspI	ATTAAT	1 cut(s)	957
XbaI	TCTAGA	2 cut(s)	3238, 3503
XceI	RCATGY	3 cut(s)	806, 3881, 4383
XcmI	CCANNNNNNNNNTGG	2 cut(s)	91, 596
XhoI	CTCGAG	1 cut(s)	4514
XmiI	GTMKAC	2 cut(s)	404, 426
XmnI	GAANNNNTTC	5 cut(s)	209, 1173, 1266, 2611, 4434
ZrmI	AGTACT	2 cut(s)	1694, 2918
Zsp2I	ATGCAT	2 cut(s)	544, 1774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.