MD08G1046300.v1.1

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001909
Physical Location & Seq
Forward (+)
3498742 .. 3509945
11204 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1046300.v1.1.491

Sequence Viewer

Length: 4968 bp
ATGGAAGTAGAGGAATCCAATTCCAGCCCCCTCAATCATGCCGCCGATGATGATCAGTCACCGCAGTCGTCCAAGAAATTCAGTCCCAGACGGTATCAGTCGGAGGTGTATGAGGTTGCGCGGCGGAAGAACACGATAGCGGTGCTGGAAACCGGTTCCGGGAAGACGATGATAGCTGTGATGCTCATCAGAGACTACGAAGGCTGCCAAACTATAAATTCAAGTAGTGGTGCCAAAAAATTGATTATTTTCTTGGCTCCAACTGTTCATCTTGTTCACCAACAATTTAAGGTTATTAAAGAAAGCACAAATTGTAGTGTGGGAGAGTATTATGGAGCCTTGGGGGTGGACTCGTGGACCATGGAGCGCTGGGAAAAGGAAATAAATGAGCACGATGTGCTAGTTATGACCCCCCAAATTCTTTTGGATGCCTTAAGAAAGGCATTCTTGAGTTTGGAAGTGGTATGCTTGATGATTGTTGATGAGTGCCATCGGGCTAATGGGAACCATCCATATACAAAGATAATGAAGGAATTTTATCACAAGTCTAGTAAAAAGCCAAGGATTTTTGGCTTGACGGCATCACCTGTTATCAAAAAAGGTGTCTCATCCACAAAGGATTGTGAGGGCCAGTTATCAGAACTTGAAAGCACTTTAGATTCCCAGATTTATACTATTAAGGACATAACAGAGATGGAAGTATGTGTCCCCTCTGCAAAAGAAACATGTAGATTTTATGGCCAAGCATGGTTTCCATGTTCAGATTTGAAAGAAAAGATGAAAGCCTCTTGGTCCAAGGTTGATGCTTCATTGTCAAAGTTGCAAGAATCAGTAGAAAGTAATGTGAATGACATGGAGGATACAATTAAGACCTTGCGGAAACGAATGTCTAATGACTACACAAAGATCTTGTATTGCCTTGATGACCTTGGTCTCATATGTACTTACGAGGCTGTTAAGATCTGTTTAGAGAATGCTCCGAAGTGCAACGAGGTGATTGAACTTTATAGAGAAGGCTCTTTGCAGTGCAGATGTTTTCTTGAGGAAGTGTTTGGTATAATTAAGGAATCATTTCTGCATGGTTCTGAAAATTTTTTGGATTTCGGGTTTGACTATTTGAAGGCAGTAGATGTGGGCTATATATCTCCAAAATTAAATGAACTTGTTCAAATTTTTCATTCTTTTGGAAGATCTAGGGAAGTACGGTGCCTCATATTTGTTGATAAAATCATCACAGCTAAAGTGATTGAGAGATTCATTAAGAAGGTTACCTCCCTGTCTCATTTTACGGTTTCCTATTTGACTGGAAGCACTACATCAGTTGACGCTCTGGCGCCGAAAATACAAAAGGAGACATTGGAGTTATTCTGCTCTGGGGCGGTCAATTTATTATTTGCTACCGATGTAGTTGAGGAGGGAATTCATGTTCTGAGCTGCTCCTGTGTGGTACGTTTCGACTTGCCAAAGACAGTGCGTAGTTATGTCCAGTCAAGGGGACGAGCTCGGCAAGATAACTCTCAATTCATCACAATGCTGGAGAGGGGAAACAAAAAACAAAGGGATCAACTATATGAGATTGTCAAGAGTGAGTGTTTGCTGACAGATACAGCTGTCAAAAGAGATCCTGAAGCATGCATTTTGAAAGAATGTACTTTGGAGGAAACAAATATATATACTGTGGATTCCACCAGAGCATTAGTCACTGCAGATTCCAGTGTCAGTCTTATACATAAATATTGTGAAACGCTGCCTAGGGATAAGTACTTCACTCCAAACCCAACTTTTCATTTCTCATATCTTGGAGGGTCTTATGAGTGTAAGATTACATTGCCTCCAAATGCGGCTTTCCAAACTTTAGTTGGCCCTGCATGCAAGAAATCTTCTTTAGCAAAGCAGCTTGTCTGCTTGGAAGCATGTAAGCAGCTACATCAAATGGGTGCCCTGAATGACCATCTTCTCCCAGTCATTGATGTGCCCGATGTGAAAAGAAAAGAGGAAGCTTCTGGTGCAGGAACTACTAAAAGGAAGGAACTACATGGTACAACTGGCATTCGTATGCTATCTGGAACTTGGGGGGAGAAACTTGATGGTGCTATTTTTCAGGCATATAAGTTTGACTTCTCCTGTAATATTGCCAGTGAATTGTATTCTGGATTTGTTTTTCTCATTGAATCGAAGCTTGCTGATGATGTGGGGAATATCAAATTGGATCTTTACCTGCTTTCGAAGACAGTTAAGGCTTCTGTTTCTTCCTGTGGGGAGGTTCATTTGGACGGGGAACAGATGGAAAAAGCAAAGTGCTTCCAGGAATTTTTCTTTAATGGATTGTTTGGGAAATTGTATGGAACCAAATCGGCTAGAAAAAAGAGAGAACTTTTACTGGAGAAAGAAACAAGAAAACTGTGGACTCCATCATGCTTATATTTGCTTCTTCCGGTTGAGACATTGAATGATTCAAAAGATGAATCCTGGAGAATAAATTGGGCAGGGATCAACTCTTGTGTTTCTGTGGTAGAGTTCTTAAAGAGAAACTCGTCTTTGGGTAGTCACCAGTGTAACGTTGACACAGGGAGTTCAGTTCCAAGTAGAACTGTCTCATCTGAGACTGAGTGCAGCAGTGCGGGGATAGTTCACTTTGCTAATTGCTCTGTTGATGTAAAAAATCTCGAAGATACGGTGGTGGTGGCTATCCATACTGGAAGAATCTACTCAGTTTATGAGGTTATGAGTAACACTTCGGCTGAGAGTCCTTTTGATGGAAAGAAAGACAATGTACCATCACAATATACTTCATTTTCTGAGTACTTCAACAAAAAATATGGGATTGTGCTCCTATATCCTGGTCAGCCTCTGTTGCGACTGAAACAAAGTCATAATCCGCACAACCTTCTTGTGAACTTCAATGGTGAAGGTGGTAATGGTAAGACATTGCAATCTGGCGTTGTTATTGGAAAGCAGCGCATGTATGCTCACATGCCACCTGAGCTTCTAGTCACTATTGGTGTCCCAAGAGACGTTCTAAAGTCATGTTATTTACTACCATCGTTGATTCATCGCTTGGAGTCCCTGATGCTAGCCAGTCAACTTAGAGAAGATATTAATTATCATTGTAGCAGCTTTGGGATATCAAGCTCATTGATTCTAGAAGCACTTACAACACTAAGATGTAATGATGATTTTTCTATGGAGCGCCTGGAATTGCTTGGTGATTCAATTTTAAAATATACCGTGAGCTGCCACCTGTTTCTCAAATATCCCAAAAAGCATGAAGGACAGTTAACTAACTATCGCATGCTGACTATTTGTAACTCAAACCTACATAGATTGGGAATGAATCGCAAGTTACAGGGATATATCCGTGATGGTGCATTTGACCCATGTCGTTGGGTTGCTCCAGGGCAGATTTCTCTACGACCCGTTCCTTGTAATTGTGGAGTGGAGACTTTAGAAGTACCATTAGATAGCAAATTCCAAACTGAAGACCCGAAAGTTGTCGTTGGAAAGAGCTGTGATAAGGGCCATAGATGGATGTGTTCAAAAACAATCTCTGATTGTGTTGAAGCACTTATAGGAGCATACTATGTTGCTGGTGGATTGCCTGCTGCCTTGCATGTGATGAAGTGGCTCGATATTGATGTAGACCTTGAACCTTCCTTGGTTGCTGATGCAATTACTACGGCATCTCTTCATTTGTACACCCCGAAAGCTAATGAGATTGCAGTCTTGGAATCAAAGCTTCACTATGAATTTTCTACCAAGGGTCTGTTGCTGGAGGCCATGACACATGCATCTGACCAAGAATCAGGAGGTGGCTACTGTTATGAGCGACTTGAATTTCTAGGTGACTCTGTGTTGGACCTGCTTATCACAAGGCATCTCTATCATAGCCACACAGATATTGATCCAGGCGAGTTAACTGACTTGCGCTCAGCTTCTGTTAATAATGAAAGTTTTGCGCAAGTTGCTGTTAGACATGACCTCCAGCAGCATCTGCAACATGCATCGATGTTACTTCTAAGTCAGATAACAGAATATGTGAAGTCTCTTTCAGAACCTGACAATGGTACCACACTTCAAGGCACCAAAGGTCCTAAGGCTCTCGGAGACATGGTGGAAAGTATTGCAGGGGCTATTCTAATTGATACCAAACTTAATCTTGATGAAGTGTGGAGAATTTTTGAGCCACTATTATCTCCAATTGTGACTCCTGACAAACTTGAGCTGCCTCCACTACGGGAGCTGATTGAATTATGCGACTTGTTCGGGTACTATAAAAAAGAGAAGTGTACCAATAAGGGTGAAACAGTTCATGCGGAGCTTACTGTACAACTGAAAGATGTTCTGTTGACTGGAGATGGGTGCGATCGGACTAAAAAAGGTGCAAAAGGAGAAGCAGCTCGTCGTTTACTCAAGAAACTTGAGGAGAGAGGTATTTCATTTTCCAAGAAGAGGAAACTGGATGCTAACCAAGTAGGTGATTCCTCTTCCCTTGACATCAACCATAAGGTCAGCGACCAAGTAACCGTCAAAGATTCTTCAGCGTTAATGGTCTATAAAAGGCGAAAGACAACTGAAATGCCTCTAGCTGCTCCACCAACTGGAGTTTCTCCTTCAGTAATTGCCAGGAAAACCTCTAGCCCCAACAATGCCGCCCCAGTCATTAAAGCAATCAACATGAAGAAAGGAGGGCCTCGATCTGCTCTTTTTGCTCTGTGCATGAAACAACAGTGGCCAAAGCCGGGCTTTAAAATAACAGAAACTCAGTCAAGAACTCCATTCACATTTGGTGAAAGCTCAACCAAGTACTTCAACAGTTTTGTCGCACAAGTAATCCTGCACATACCCAACTCCGGCGTCATTGAATGCACAGGAGATGCTAGACCTGACAAGGGGAGTTCAGAAGATTCTGCAGCCGTCGTCTTGTTTCAAGAGCTTCAACGGCAGGGAAGAGTTGTAATTGCTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009892 GO:0009987 GO:0010033 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0031974 GO:0031981 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0098586 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1656

Amino Acids

184.58

Weight (kDa)

6.76

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 27 - 196 9.6e-17 Type III restriction enzyme, res subunit
DEAD PF00270 31 - 196 2e-19 DEAD/DEAH box helicase
Helicase_C PF00271 384 - 501 4.5e-14 Helicase conserved C-terminal domain
Dicer_dimer PF03368 572 - 655 1.4e-25 Dicer dimerisation domain
PAZ PF02170 887 - 998 5.8e-10 PAZ domain
Ribonuclease_3 PF00636 1063 - 1198 1.1e-17 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1254 - 1381 6.4e-15 Ribonuclease-III-like
Ribonuclease_3 PF00636 1275 - 1382 1.4e-21 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 3957
Acc36I ACCTGC 2 cut(s) 2226, 3867
Acc65I GGTACC 1 cut(s) 4064
AccB1I GGYRCC 6 cut(s) 230, 1206, 1333, 1937, 4064, 4079
AccB7I CCANNNNNTGG 1 cut(s) 4598
AccI GTMKAC 1 cut(s) 3639
AccII CGCG 1 cut(s) 121
AclI AACGTT 1 cut(s) 2559
AclWI GGATC 5 cut(s) 1569, 1616, 2217, 2498, 3896
AcoI YGGCCR 2 cut(s) 739, 4730
AcuI CTGAAG 4 cut(s) 1647, 3498, 4521, 4596
AcyI GRCGYC 2 cut(s) 1334, 4854
AdeI CACNNNGTG 1 cut(s) 397
AfeI AGCGCT 1 cut(s) 368
AfiI CCNNNNNNNGG 9 cut(s) 159, 1492, 2756, 4061, 4449, 4598, 4739, 4850, 4889
AflII CTTAAG 1 cut(s) 433
AflIII ACRYGT 1 cut(s) 725
AgeI ACCGGT 1 cut(s) 152
AjnI CCWGG 7 cut(s) 2304, 2468, 2839, 3192, 3394, 3904, 4622
AjuI GAANNNNNNNTTGG 6 cut(s) 735, 767, 2189, 2221, 4588, 4620
AloI GAACNNNNNNTCC 2 cut(s) 1410, 1442
Alw21I GWGCWC 3 cut(s) 393, 1504, 2832
AlwI GGATC 5 cut(s) 1569, 1616, 2217, 2498, 3896
AlwNI CAGNNNCTG 4 cut(s) 2851, 3935, 3991, 4055
Aor51HI AGCGCT 1 cut(s) 368
AoxI GGCC 7 cut(s) 628, 739, 1861, 3517, 3774, 4688, 4730
ArsI GACNNNNNNTTYG 2 cut(s) 588, 620
AseI ATTAAT 1 cut(s) 3099
AsiGI ACCGGT 1 cut(s) 152
Asp700I GAANNNNTTC 3 cut(s) 1071, 1164, 4305
Asp718I GGTACC 1 cut(s) 4064
AspA2I CCTAGG 1 cut(s) 1751
AspLEI GCGC 7 cut(s) 121, 369, 1336, 2961, 3192, 3927, 3958
AspS9I GGNCC 8 cut(s) 357, 628, 792, 1862, 3517, 3856, 4088, 4688
AsuC2I CCSGG 2 cut(s) 160, 4740
AsuII TTCGAA 1 cut(s) 2225
AsuNHI GCTAGC 1 cut(s) 3073
AvaII GGWCC 4 cut(s) 357, 792, 3856, 4088
AvrII CCTAGG 1 cut(s) 1751
AxyI CCTNAGG 1 cut(s) 4092
BaeGI GKGCMC 2 cut(s) 1942, 1977
BaeI ACNNNNGTAYC 6 cut(s) 1596, 1629, 2664, 2697, 4048, 4081
BalI TGGCCA 2 cut(s) 741, 4732
BanI GGYRCC 6 cut(s) 230, 1206, 1333, 1937, 4064, 4079
BanII GRGCYC 1 cut(s) 1504
BauI CACGAG 1 cut(s) 352
BbsI GAAGAC 3 cut(s) 170, 2234, 3486
Bbv12I GWGCWC 3 cut(s) 393, 1504, 2832
BceAI ACGGC 4 cut(s) 594, 3693, 4898, 4955
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BciT130I CCWGG 7 cut(s) 2306, 2470, 2841, 3194, 3396, 3906, 4624
BciVI GTATCC 1 cut(s) 853
BclI TGATCA 1 cut(s) 52
BcnI CCSGG 2 cut(s) 160, 4740
BfmI CTRYAG 2 cut(s) 1704, 4908
BfoI RGCGCY 3 cut(s) 370, 1337, 3193
BfrI CTTAAG 1 cut(s) 433
BfuAI ACCTGC 2 cut(s) 2226, 3867
BfuI GTATCC 1 cut(s) 853
BglII AGATCT 3 cut(s) 906, 960, 1190
BlnI CCTAGG 1 cut(s) 1751
BlpI GCTNAGC 1 cut(s) 3928
BmcAI AGTACT 3 cut(s) 1763, 2804, 4805
Bme1390I CCNGG 9 cut(s) 160, 2306, 2470, 2841, 3194, 3396, 3906, 4624, 4740
Bme18I GGWCC 4 cut(s) 357, 792, 3856, 4088
BmgT120I GGNCC 8 cut(s) 357, 628, 792, 1862, 3517, 3856, 4088, 4688
BmrFI CCNGG 9 cut(s) 160, 2306, 2470, 2841, 3194, 3396, 3906, 4624, 4740
BmrI ACTGGG 2 cut(s) 1955, 4649
BmtI GCTAGC 1 cut(s) 3077
BmuI ACTGGG 2 cut(s) 1955, 4649
BoxI GACNNNNGTC 2 cut(s) 930, 3378
BpiI GAAGAC 3 cut(s) 170, 2234, 3486
BpmI CTGGAG 8 cut(s) 1556, 2402, 2491, 3378, 3791, 3965, 4371, 4620
Bpu10I CCTNAGC 1 cut(s) 2982
Bpu1102I GCTNAGC 1 cut(s) 3928
Bpu14I TTCGAA 1 cut(s) 2225
BpuEI CTTGAG 5 cut(s) 469, 1061, 4238, 4394, 4439
BpuMI CCSGG 2 cut(s) 160, 4740
Bsa29I ATCGAT 1 cut(s) 4004
BsaBI GATNNNNATC 3 cut(s) 51, 185, 3900
BsaHI GRCGYC 2 cut(s) 1334, 4854
BsaI GGTCTC 1 cut(s) 938
BsaJI CCNNGG 9 cut(s) 339, 360, 560, 795, 928, 1751, 3395, 3654, 3756
BsaWI WCCGGW 2 cut(s) 152, 2434
BsaXI ACNNNNNCTCC 6 cut(s) 1816, 1846, 3963, 3993, 4095, 4125
Bsc4I CCNNNNNNNGG 9 cut(s) 159, 1492, 2756, 4061, 4449, 4598, 4739, 4850, 4889
Bse118I RCCGGY 1 cut(s) 152
Bse21I CCTNAGG 1 cut(s) 4092
Bse3DI GCAATG 2 cut(s) 1826, 2927
Bse8I GATNNNNATC 3 cut(s) 51, 185, 3900
BseBI CCWGG 7 cut(s) 2306, 2470, 2841, 3194, 3396, 3906, 4624
BseCI ATCGAT 1 cut(s) 4004
BseDI CCNNGG 9 cut(s) 339, 360, 560, 795, 928, 1751, 3395, 3654, 3756
BseGI GGATG 5 cut(s) 433, 508, 608, 3534, 4465
BseJI GATNNNNATC 3 cut(s) 51, 185, 3900
BseLI CCNNNNNNNGG 9 cut(s) 159, 1492, 2756, 4061, 4449, 4598, 4739, 4850, 4889
BseMI GCAATG 2 cut(s) 1826, 2927
BseMII CTCAG 9 cut(s) 1421, 2592, 2598, 2724, 2733, 2790, 2973, 3942, 4775
BseRI GAGGAG 2 cut(s) 1427, 4436
BseSI GKGCMC 2 cut(s) 1942, 1977
BseYI CCCAGC 2 cut(s) 369, 4961
BsgI GTGCAG 4 cut(s) 1048, 2028, 2632, 4820
Bsh1236I CGCG 1 cut(s) 121
Bsh1285I CGRYCG 1 cut(s) 4366
BshFI GGCC 7 cut(s) 630, 741, 1863, 3519, 3776, 4690, 4732
BshNI GGYRCC 6 cut(s) 230, 1206, 1333, 1937, 4064, 4079
BshTI ACCGGT 1 cut(s) 152
BshVI ATCGAT 1 cut(s) 4004
BsiEI CGRYCG 1 cut(s) 4366
BsiHKAI GWGCWC 3 cut(s) 393, 1504, 2832
BsiSI CCGG 5 cut(s) 153, 159, 2435, 4739, 4851
BslFI GGGAC 5 cut(s) 69, 692, 1509, 2990, 3049
BslI CCNNNNNNNGG 9 cut(s) 159, 1492, 2756, 4061, 4449, 4598, 4739, 4850, 4889
BsmBI CGTCTC 1 cut(s) 3006
BsmFI GGGAC 5 cut(s) 69, 692, 1509, 2990, 3049
BsmI GAATGC 4 cut(s) 443, 979, 2049, 4868
BsnI GGCC 7 cut(s) 630, 741, 1863, 3519, 3776, 4690, 4732
Bso31I GGTCTC 1 cut(s) 938
Bsp119I TTCGAA 1 cut(s) 2225
Bsp1286I GDGCHC 5 cut(s) 393, 1504, 1942, 1977, 2832
Bsp1407I TGTACA 2 cut(s) 3693, 4324
Bsp1720I GCTNAGC 1 cut(s) 3928
Bsp19I CCATGG 1 cut(s) 360
BspANI GGCC 7 cut(s) 630, 741, 1863, 3519, 3776, 4690, 4732
BspCNI CTCAG 9 cut(s) 1422, 2593, 2599, 2723, 2734, 2791, 2974, 3941, 4774
BspDI ATCGAT 1 cut(s) 4004
BspFNI CGCG 1 cut(s) 121
BspMAI CTGCAG 2 cut(s) 1708, 4912
BspMI ACCTGC 2 cut(s) 2226, 3867
BspOI GCTAGC 1 cut(s) 3077
BspPI GGATC 5 cut(s) 1569, 1616, 2217, 2498, 3896
BspT104I TTCGAA 1 cut(s) 2225
BspT107I GGYRCC 6 cut(s) 230, 1206, 1333, 1937, 4064, 4079
BspTI CTTAAG 1 cut(s) 433
BspTNI GGTCTC 1 cut(s) 938
BsrDI GCAATG 2 cut(s) 1826, 2927
BsrFI RCCGGY 1 cut(s) 152
BsrGI TGTACA 2 cut(s) 3693, 4324
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 9 cut(s) 339, 360, 560, 795, 928, 1751, 3395, 3654, 3756
BssNI GRCGYC 2 cut(s) 1334, 4854
BssSI CACGAG 1 cut(s) 352
BssT1I CCWWGG 8 cut(s) 339, 360, 560, 795, 928, 1751, 3654, 3756
Bst2BI CACGAG 1 cut(s) 352
Bst2UI CCWGG 7 cut(s) 2306, 2470, 2841, 3194, 3396, 3906, 4624
Bst6I CTCTTC 4 cut(s) 3690, 4442, 4489, 4942
BstACI GRCGYC 2 cut(s) 1334, 4854
BstAFI CTTAAG 1 cut(s) 433
BstAPI GCANNNNNTGC 2 cut(s) 397, 3991
BstAUI TGTACA 2 cut(s) 3693, 4324
BstBI TTCGAA 1 cut(s) 2225
BstC8I GCNNGC 6 cut(s) 1633, 1870, 2181, 3075, 3293, 3600
BstDSI CCRYGG 1 cut(s) 360
BstEII GGTNACC 1 cut(s) 1267
BstF5I GGATG 5 cut(s) 433, 508, 608, 3534, 4465
BstFNI CGCG 1 cut(s) 121
BstH2I RGCGCY 3 cut(s) 370, 1337, 3193
BstHHI GCGC 7 cut(s) 121, 369, 1336, 2961, 3192, 3927, 3958
BstMCI CGRYCG 1 cut(s) 4366
BstMWI GCNNNNNNNGC 9 cut(s) 397, 1869, 2006, 2854, 2983, 3962, 3991, 4706, 4939
BstNI CCWGG 7 cut(s) 2306, 2470, 2841, 3194, 3396, 3906, 4624
BstPAI GACNNNNGTC 2 cut(s) 930, 3378
BstPI GGTNACC 1 cut(s) 1267
BstSCI CCNGG 9 cut(s) 158, 2304, 2468, 2839, 3192, 3394, 3904, 4622, 4738
BstSFI CTRYAG 2 cut(s) 1704, 4908
BstSLI GKGCMC 2 cut(s) 1942, 1977
BstUI CGCG 1 cut(s) 121
BstV2I GAAGAC 3 cut(s) 170, 2234, 3486
BstX2I RGATCY 5 cut(s) 906, 960, 1190, 1621, 2209
BstXI CCANNNNNNTGG 1 cut(s) 3384
BstYI RGATCY 5 cut(s) 906, 960, 1190, 1621, 2209
Bsu15I ATCGAT 1 cut(s) 4004
Bsu36I CCTNAGG 1 cut(s) 4092
BsuI GTATCC 1 cut(s) 853
BsuRI GGCC 7 cut(s) 630, 741, 1863, 3519, 3776, 4690, 4732
BsuTUI ATCGAT 1 cut(s) 4004
BtgI CCRYGG 1 cut(s) 360
BtgZI GCGATG 1 cut(s) 3038
BtsCI GGATG 5 cut(s) 433, 508, 608, 3534, 4465
BtsI GCAGTG 3 cut(s) 1031, 1701, 2623
BtsIMutI CAGTG 8 cut(s) 1031, 1476, 1701, 1720, 2143, 2558, 2623, 4733
BveI ACCTGC 2 cut(s) 2226, 3867
Cac8I GCNNGC 6 cut(s) 1633, 1870, 2181, 3075, 3293, 3600
CaiI CAGNNNCTG 4 cut(s) 2851, 3935, 3991, 4055
CfoI GCGC 7 cut(s) 121, 369, 1336, 2961, 3192, 3927, 3958
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 8 cut(s) 357, 628, 792, 1862, 3517, 3856, 4088, 4688
ClaI ATCGAT 1 cut(s) 4004
CseI GACGC 2 cut(s) 1334, 4843
CspAI ACCGGT 1 cut(s) 152
DinI GGCGCC 1 cut(s) 1335
DraI TTTAAA 2 cut(s) 3219, 4747
DraIII CACNNNGTG 1 cut(s) 397
EaeI YGGCCR 2 cut(s) 739, 4730
Eam1104I CTCTTC 4 cut(s) 3690, 4442, 4489, 4942
EarI CTCTTC 4 cut(s) 3690, 4442, 4489, 4942
EciI GGCGGA 1 cut(s) 139
Ecl136II GAGCTC 1 cut(s) 1502
Eco130I CCWWGG 8 cut(s) 339, 360, 560, 795, 928, 1751, 3654, 3756
Eco24I GRGCYC 1 cut(s) 1504
Eco31I GGTCTC 1 cut(s) 938
Eco32I GATATC 1 cut(s) 3126
Eco47I GGWCC 4 cut(s) 357, 792, 3856, 4088
Eco47III AGCGCT 1 cut(s) 368
Eco53kI GAGCTC 1 cut(s) 1502
Eco57I CTGAAG 4 cut(s) 1647, 3498, 4521, 4596
Eco81I CCTNAGG 1 cut(s) 4092
Eco91I GGTNACC 1 cut(s) 1267
EcoICRI GAGCTC 1 cut(s) 1502
EcoO109I RGGNCCY 2 cut(s) 4088, 4688
EcoO65I GGTNACC 1 cut(s) 1267
EcoRI GAATTC 1 cut(s) 1419
EcoRII CCWGG 7 cut(s) 2304, 2468, 2839, 3192, 3394, 3904, 4622
EcoRV GATATC 1 cut(s) 3126
EcoT14I CCWWGG 8 cut(s) 339, 360, 560, 795, 928, 1751, 3654, 3756
EcoT22I ATGCAT 3 cut(s) 1637, 3790, 4003
EcoT38I GRGCYC 1 cut(s) 1504
EgeI GGCGCC 1 cut(s) 1335
EheI GGCGCC 1 cut(s) 1335
ErhI CCWWGG 8 cut(s) 339, 360, 560, 795, 928, 1751, 3654, 3756
Esp3I CGTCTC 1 cut(s) 3006
FalI AAGNNNNNCTT 6 cut(s) 431, 463, 2102, 2134, 4727, 4759
FaqI GGGAC 5 cut(s) 69, 692, 1509, 2990, 3049
FauI CCCGC 1 cut(s) 2614
FauNDI CATATG 1 cut(s) 938
FbaI TGATCA 1 cut(s) 52
FblI GTMKAC 1 cut(s) 3639
FokI GGATG 5 cut(s) 440, 495, 595, 3541, 4472
FriOI GRGCYC 1 cut(s) 1504
FspI TGCGCA 1 cut(s) 3957
GlaI GCGC 7 cut(s) 120, 368, 1335, 2960, 3191, 3926, 3957
GsaI CCCAGC 2 cut(s) 373, 4965
GsuI CTGGAG 8 cut(s) 1556, 2402, 2491, 3378, 3791, 3965, 4371, 4620
HaeII RGCGCY 3 cut(s) 370, 1337, 3193
HaeIII GGCC 7 cut(s) 630, 741, 1863, 3519, 3776, 4690, 4732
HapII CCGG 5 cut(s) 153, 159, 2435, 4739, 4851
HgaI GACGC 2 cut(s) 1334, 4843
HhaI GCGC 7 cut(s) 121, 369, 1336, 2961, 3192, 3927, 3958
Hin1I GRCGYC 2 cut(s) 1334, 4854
Hin6I GCGC 7 cut(s) 119, 367, 1334, 2959, 3190, 3925, 3956
HinP1I GCGC 7 cut(s) 119, 367, 1334, 2959, 3190, 3925, 3956
HincII GTYRAC 6 cut(s) 1324, 2563, 3083, 3279, 3915, 4347
HindII GTYRAC 6 cut(s) 1324, 2563, 3083, 3279, 3915, 4347
HindIII AAGCTT 3 cut(s) 1998, 2177, 3734
HpaI GTTAAC 2 cut(s) 3279, 3915
HpaII CCGG 5 cut(s) 153, 159, 2435, 4739, 4851
Hpy99I CGWCG 2 cut(s) 4404, 4919
HpyCH4IV ACGT 3 cut(s) 1450, 2559, 3015
HpyF10VI GCNNNNNNNGC 9 cut(s) 397, 1869, 2006, 2854, 2983, 3962, 3991, 4706, 4939
HpySE526I ACGT 3 cut(s) 1450, 2559, 3015
Hsp92I GRCGYC 2 cut(s) 1334, 4854
HspAI GCGC 7 cut(s) 119, 367, 1334, 2959, 3190, 3925, 3956
KasI GGCGCC 1 cut(s) 1333
KpnI GGTACC 1 cut(s) 4068
Ksp22I TGATCA 1 cut(s) 52
KspAI GTTAAC 2 cut(s) 3279, 3915
MaeII ACGT 3 cut(s) 1450, 2559, 3015
MfeI CAATTG 1 cut(s) 4197
MflI RGATCY 5 cut(s) 906, 960, 1190, 1621, 2209
MhlI GDGCHC 5 cut(s) 393, 1504, 1942, 1977, 2832
MlsI TGGCCA 2 cut(s) 741, 4732
MluNI TGGCCA 2 cut(s) 741, 4732
Mly113I GGCGCC 1 cut(s) 1334
MlyI GAGTC 6 cut(s) 344, 2401, 2755, 3071, 3839, 4198
MmeI TCCRAC 4 cut(s) 81, 284, 3478, 3834
Mox20I TGGCCA 2 cut(s) 741, 4732
Mph1103I ATGCAT 3 cut(s) 1637, 3790, 4003
MroXI GAANNNNTTC 3 cut(s) 1071, 1164, 4305
MscI TGGCCA 2 cut(s) 741, 4732
MslI CAYNNNNRTG 4 cut(s) 1529, 1970, 2054, 3163
Msp20I TGGCCA 2 cut(s) 741, 4732
MspA1I CMGCKG 1 cut(s) 1610
MspCI CTTAAG 1 cut(s) 433
MspI CCGG 5 cut(s) 153, 159, 2435, 4739, 4851
MspR9I CCNGG 9 cut(s) 160, 2306, 2470, 2841, 3194, 3396, 3906, 4624, 4740
MunI CAATTG 1 cut(s) 4197
Mva1269I GAATGC 4 cut(s) 443, 979, 2049, 4868
MvaI CCWGG 7 cut(s) 2306, 2470, 2841, 3194, 3396, 3906, 4624
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 9 cut(s) 397, 1869, 2006, 2854, 2983, 3962, 3991, 4706, 4939
NarI GGCGCC 1 cut(s) 1334
NciI CCSGG 2 cut(s) 160, 4740
NcoI CCATGG 1 cut(s) 360
NdeI CATATG 1 cut(s) 938
NheI GCTAGC 1 cut(s) 3073
NmeAIII GCCGAG 1 cut(s) 1483
NmuCI GTSAC 6 cut(s) 57, 1699, 2546, 2992, 3842, 4201
NsbI TGCGCA 1 cut(s) 3957
NsiI ATGCAT 3 cut(s) 1637, 3790, 4003
NspV TTCGAA 1 cut(s) 2225
PaeI GCATGC 3 cut(s) 1635, 1872, 3295
PciI ACATGT 1 cut(s) 725
PctI GAATGC 4 cut(s) 443, 979, 2049, 4868
PdmI GAANNNNTTC 3 cut(s) 1071, 1164, 4305
PflMI CCANNNNNTGG 1 cut(s) 4598
PfoI TCCNGGA 3 cut(s) 158, 2304, 2468
PinAI ACCGGT 1 cut(s) 152
Ple19I CGATCG 1 cut(s) 4366
PleI GAGTC 6 cut(s) 344, 2401, 2754, 3070, 3839, 4198
PluTI GGCGCC 1 cut(s) 1337
PpsI GAGTC 6 cut(s) 344, 2401, 2754, 3070, 3839, 4198
PpuMI RGGWCCY 1 cut(s) 4088
PscI ACATGT 1 cut(s) 725
PshAI GACNNNNGTC 2 cut(s) 930, 3378
PshBI ATTAAT 1 cut(s) 3099
Psp124BI GAGCTC 1 cut(s) 1504
Psp1406I AACGTT 1 cut(s) 2559
Psp5II RGGWCCY 1 cut(s) 4088
Psp6I CCWGG 7 cut(s) 2304, 2468, 2839, 3192, 3394, 3904, 4622
PspEI GGTNACC 1 cut(s) 1267
PspFI CCCAGC 2 cut(s) 369, 4961
PspGI CCWGG 7 cut(s) 2304, 2468, 2839, 3192, 3394, 3904, 4622
PspPI GGNCC 8 cut(s) 357, 628, 792, 1862, 3517, 3856, 4088, 4688
PspPPI RGGWCCY 1 cut(s) 4088
PstI CTGCAG 2 cut(s) 1708, 4912
PstNI CAGNNNCTG 4 cut(s) 2851, 3935, 3991, 4055
PsuI RGATCY 5 cut(s) 906, 960, 1190, 1621, 2209
PvuI CGATCG 1 cut(s) 4366
PvuII CAGCTG 1 cut(s) 1610
RseI CAYNNNNRTG 4 cut(s) 1529, 1970, 2054, 3163
SacI GAGCTC 1 cut(s) 1504
Sau96I GGNCC 8 cut(s) 357, 628, 792, 1862, 3517, 3856, 4088, 4688
ScaI AGTACT 3 cut(s) 1763, 2804, 4805
SchI GAGTC 6 cut(s) 344, 2401, 2755, 3071, 3839, 4198
ScrFI CCNGG 9 cut(s) 160, 2306, 2470, 2841, 3194, 3396, 3906, 4624, 4740
SduI GDGCHC 5 cut(s) 393, 1504, 1942, 1977, 2832
SfcI CTRYAG 2 cut(s) 1704, 4908
SfoI GGCGCC 1 cut(s) 1335
SfuI TTCGAA 1 cut(s) 2225
SinI GGWCC 4 cut(s) 357, 792, 3856, 4088
SmiMI CAYNNNNRTG 4 cut(s) 1529, 1970, 2054, 3163
SmlI CTYRAG 6 cut(s) 433, 448, 1040, 4217, 4409, 4418
SmoI CTYRAG 6 cut(s) 433, 448, 1040, 4217, 4409, 4418
SphI GCATGC 3 cut(s) 1635, 1872, 3295
SspDI GGCGCC 1 cut(s) 1333
SspI AATATT 2 cut(s) 1736, 2131
SstI GAGCTC 1 cut(s) 1504
StyD4I CCNGG 9 cut(s) 158, 2304, 2468, 2839, 3192, 3394, 3904, 4622, 4738
StyI CCWWGG 8 cut(s) 339, 360, 560, 795, 928, 1751, 3654, 3756
TaiI ACGT 3 cut(s) 1453, 2562, 3018
TaqI TCGA 7 cut(s) 1455, 2174, 2225, 2667, 3627, 4004, 4693
TatI WGTACW 7 cut(s) 941, 1649, 1761, 2802, 3693, 4324, 4803
TauI GCSGC 4 cut(s) 44, 124, 1844, 4652
TscAI CASTG 8 cut(s) 1031, 1476, 1708, 1720, 2143, 2558, 2623, 4733
TseFI GTSAC 6 cut(s) 57, 1699, 2546, 2992, 3842, 4201
Tsp45I GTSAC 6 cut(s) 57, 1699, 2546, 2992, 3842, 4201
TspGWI ACGGA 1 cut(s) 3347
TspRI CASTG 8 cut(s) 1031, 1476, 1708, 1720, 2143, 2558, 2623, 4733
Van91I CCANNNNNTGG 1 cut(s) 4598
Vha464I CTTAAG 1 cut(s) 433
VpaK11BI GGWCC 4 cut(s) 357, 792, 3856, 4088
VspI ATTAAT 1 cut(s) 3099
XbaI TCTAGA 1 cut(s) 3142
XcmI CCANNNNNNNNNTGG 2 cut(s) 497, 1856
XmaJI CCTAGG 1 cut(s) 1751
XmiI GTMKAC 1 cut(s) 3639
XmnI GAANNNNTTC 3 cut(s) 1071, 1164, 4305
ZrmI AGTACT 3 cut(s) 1763, 2804, 4805
Zsp2I ATGCAT 3 cut(s) 1637, 3790, 4003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.