Rh6CG526600

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0001909
Physical Location & Seq
Forward (+)
68104833 .. 68117938
13106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG526600.1

Sequence Viewer

Length: 4878 bp
ATGCATTTGCCAGAGGAAGAAGGCCAGAATTCCCTGAAACGCAGCTTCACTGAAACCACTGATGATCATGGTGCCGCCGCCGATCAAGGAGTTTCAAAGCGCCTCAATCCCAGAAGGTATCAGACACAAGTGTATGAGGTTGCTAGGAAGAGGAACACAATAGCTGTGATGGAGACAGGTACTGGCAAGACAATGATAGCTGTGATGCTCATTGATGAAATTGGTCAAGCTATCAAGTCATCTGGTGACAACAAATTGATCATATTCTTGGCCCCAACTGTTCATCTTGTTCATCAGCAATTTGAGGTTATTAAAGAACACACTACTTTTAAAGTAGAGGAGTATTATGGAGCCAAGGGGGTTGATGCGTGGGATAAGGAGCATTGGGAAAAGGAATTTAAAACCCATGATGTACTGGTTATGACACCCCAGATTCTTTTGGATGCCTTAAGAAAGGCATTCTTGACCATAGAAGCAGTATGCTTGATGATTATGGATGAGTGCCATCGGGCTACCGGAAACCACCCTTATACAAAGATAATGAAGGAGTTTTATCACAGGTCTAGTATCAAGCCAAAAGTTTTTGGAATGACGGCTTCTCCTGTAATTCGTAAAGGTGTCTCATCCACAATGGACTGTGAAGATCAAATATCTGAACTTGAAAGCATTTTGGATTCCCAGATTTATACTATAGAGGATAAAACAGAGATGGAAGTATATGCCCCTACTGCAAAAGAAACTCGTAGATTTTATAACCCAACATGGTTTTCGAGTTTGGATTTGAAAGAAAAGATCAAGGCCTCTTGGTCGAAGAATGATGCTTCGTTTTTAAAGCTGCAAGAATCAGTTCAAAGTAATTTCAAAGATTTGGATGACAAAGTTAAGGTCCTGCGGAAACGATTGTCTAATGACTATGAAAAGATCCTGTATTGCCTGGACTACCTTGGTCTCATATGTGCTTATGAGGCTGTGAAGGTCTGTCTAGAGAATGCTCCTACTAGCGAAGAGTGTGAATTTTATAGACAAAGTTCTTTGCAGTGCAGATATTTTTTTGAGGAAGTTTTTGGTATAATCAAGCAATCTCTTTCGAATGGTTGTGAAAATTTTTTGGATGTTGGGTTTGACTATTTGAAGGCAGTAGATTTGGGCTACATATCTCCAAAGTTAAATGAACTTGTTCAGCTTTTCCATTCGTTTGGAGTATCTAGGGAACTACTGTGCCTCATCTTTGTGGATAGAATTGTCACTGCTAAAGTGATTGAAAGATTTATGAGAAAAGTTACCTCCTTATCTCATTTCAAAATTTCCTATTTGACTGGGAGTACTACATCAGTTGATGCTCTGACACCAAAAGTACAAAAGGAGACATTGAAGTTATTCAGCTCTGGGGAGGTCAATTTATTATTTTCTACCGATGTAGTTGAGGAGGGAATTCATGTTCCAAACTGCTCCTGTGTGATACGTTTTGACTTGCCAAAGACAGTGCGTAGTTATGTCCAGTCCAGAGGACGGGCTCGTCAAGATAACTCTCAATTCATTATAATGCTAGAAAGGGGAAACAAGCAACAGAGGGATCAACTATATGAGATCATCAAGAGCGAAGGTTTGATGACAAATACAGCTCTAAATAGAGATCCTGAAGAAAGCGTTTTGAAACCATGTACACTTGATGAAATAAGTGAATATGTTGTGAATGCCACTGGAGCATCAGTTACAGCAGATTCCAGTGTCCGTACTATACATAAGTATTGTGACACGCTTCCCAGAGACAAGTACTTTGTTCCAAGACCAGCATTTCAATTTTCATATCTCGGAGATTCTTATGAATGTAAGATTACATTGCCTCCAAATGCGGCTTTCCAAACCTTGGTCGGTCCGGCATGCAAAAACTCCGCTTTATCAAAGCAGGTTGTATGCTTAGAAGCTTGTAAGAAGCTGCATCAATTGGGTGCCTTGAATGATCATCTTCTCCCGTCCACTGATAAGCCTTTGGAGAAAGATATCAATGTAGAAAGCAAAGGGCCAGCTTCTGGTGCAGGAACAACTAAAAGGAAGGAACTACATGGGACAACTGGCATTCGCGCTCTGTCAGGAACTTGGGCAGAAAAACTTGATGGTGCCGTTTTTTATGCTTATAAATTTGACTTCTCCTGTGATATTGTTACCGAGCTATATTCTGGATTTATTCTTCTAATTGAGTCAGAGCTTGCTAAGGATGTGGGGAATATTGAATTGACGCTTTACTTGGTTTCAAAGAAAGTTAAAGCTTCTGTTTCTTCCTGTGGGCAAGTTCGTTTGGATGGAGAACTGATGGCAAAAGCAAAGCTCTTTCAGGAATTTTTCTTCAATGGATTGTTTGGGAAATTGTTTCATCGAGCCAACCCAGCTGAAAAGCAGAGAGAGTTTTTACTTATGAAAGAAACAAGAAAGCTGTGGAGGCAATCATACATGTATTTGGTTCTTCCGCTTGAAACATTGGATGATTCAAATAATGAATCTTGGACAATAAATTGGGGAGGGATCAGTTCTTGTGTTTCTGTGGTAGAGTTCTTGAAGCAAAATGCATTGTTGGGTGCTCAGCATTGTAAGGGTGATGCAAGGAATTCATTGAAAAGCAGAAGTGGCGCATCTGGGACTGGATGCAATAGTTCACAGATATTCCACTTTGCTGATTCTTCTGTTAATGAAAAAAATCTCAAAGATATGGTGGTAGTGGCCATCCACACCGGAAAAATATATTCTGTTTTTGAGGTTTTGAGTGACACATCCGCTGAGAGTCCTTTTGACACATCACAGTACAATACCTATGCTGAGTACTTCCACAAAAAATATGGGGTTCTGCTCCTGTATCCGGGTCAGCCTCTGTTGCGGCTGAAGCAAAATCATAATCCACACAACCTTCTTGTGAACTTCAATGGCGAAGGTGGTAGTGGTAAGACATCAGAATCTGGCCTTGTTAATGGAAAGGAACGTATGTATGCCCATATGCCTCCTGAGCTTCTAGTCTGTACTGGATTGCGAAGAGACGTTTTGAAGTCATTTTATCTACTACCATCATTGATTCATCGATTGGAATCCTTGATGTTAGCCAGCCAACTCAGAGAAGAGATTGACAGTCATTGCAGCTTACAGATATCAAGCTCATTGATTCTAGAAGCACTTACATCACTCAGATGCAGCGAAGATTTTTCCATGGAGCGTTTGGAATTGCTTGGTGATTCAGTGCTAAAGTATACTGTAGGCTGCCACCTCTTCCTAAAATATCCCGAAAAACATGATGGACAATTATCTTCTCTGCGCCAGTCTGTAATTTGTAACGCTAACCTGCATAAATTGGGAATAGATCGAAACTTACAGGGATATATACGTGATGGTGCTTTTGATCCACGCCGTTGGGTTGGTCCAGGACAGGTCTCTATACGACCTGTTCCTTGTGTGTGTGGAGTGGATACCTTAGAAGTTCCAGTAGATAGCAAGTTTCAAACTAGAGACCGCGTAGTTGTGGTTGGAAAGTGCTGTGACAAGGGCCATAGATGGATGGGTTCCAAAACAATCTCTGATTGCGTTGAAGCCCTTATAGGAGCTTACTATGTAGCTGGTGGATTATCTGCTGCAGTCCATTTTATGAAGTGGCTCCAAATTGATGTAGAACTTGAGCCATCCTTGGTTTCTGAAGCAATTACTACTGCATCACTTCATTCCTGCAACCCAAAAGTGGATGAGATTGGAATCCTAGAGTCAAAGCTTCGTTATGTATTTTCTACCAAGGGTCTCTTACAGGAAGCTATAACGCATGCATCTGAGCAAGAATCAAAACTTGGCTATTCTTATGAGAGACTAGAGTTTCTCGGTGATTGTGTGTTGGACCTGCTTATCACATGGTATCTCTATCAGAGCCACAAAGATATTGATCCAGGCGAGTTAACTGACTTGCGTGCCGCTTCAGTTAACAATGAAAACTTTGCTCAAGCTGCTGTGAGACACAACCTTCAACAGCATCTACAACATTGCTCTGGGTTACTTCAAAGTCAAATAACAGAATATGTAAAGTTGCTTTCTGAACATGATAGTGGTGACAAACTTCAAGGGCCAAAAGGTCCTAAGGCTCTTGGAGACATGGTGGAAAGTATTGCAGGGGCAATACTAATTGATACGGAGCTCAACCTTGACGAAGTGTGGAGAGTATACAAACCACTGTTATCTCCAATAGTGACCCCTGATAAACTTGAGCTGCCTCCCTTGCGTGAACTGATTGAATTATGTGGCTCACTTGGGTACTTTTATAAAGAACAAACTGTAAAGAAGGGTGAAACAGTTCACGCGGAGCTTAGTGTACAGCTGAAAGATGTCCTGTTGATTGGAGAGGGGACTGATCGGAGTAAGAAAATCGCAAAAGGAGAAGCAGCTCATCATTTATTGAAGGAGCTTGAGAAAAGAGGTATCACACACTCTGGGTCTGCCAGGAAGAGAAAACTGGGTGCTGACCAAGTTGGTGATTCATCTCCAATAAAGCAAAAGAAAACTGAGATGCATCTGCCTATGGATACAACTGGACTTTCTTTGCCAGCCACTGACAGAAAAATAAGTAGTTCGAAAAATGACATCCCAGTTATCAAAGCAATCAACATGAAGAAAGGAGGACCTCGAGTCTCTCTTTACGAGCTCTGCAAACAACTACAATGGCAAATGCCTACCTTCAAACCAACAGAAAAGGATTCCAGTTTCATATCAACAATAACGTTGTACATACCCAATTTGGGCAACATCGAATGCACAGGAGATCCTAGGTCAGATAAGAAGAGTTCACTAGACTCTGCAGCACTTCTCATGCTTTATGATCTCGAAAGGCAGGGTAAGATTATCATCGGTGGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009892 GO:0009987 GO:0010033 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0031974 GO:0031981 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0098586 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1625

Amino Acids

182.3

Weight (kDa)

6.55

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 35 - 201 4e-18 Type III restriction enzyme, res subunit
DEAD PF00270 39 - 201 5.3e-20 DEAD/DEAH box helicase
Helicase_C PF00271 389 - 505 2.6e-17 Helicase conserved C-terminal domain
Dicer_dimer PF03368 576 - 662 1.8e-23 Dicer dimerisation domain
PAZ PF02170 892 - 1019 4.9e-11 PAZ domain
Ribonucleas_3_3 PF14622 1064 - 1123 4.8e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 1066 - 1201 4.2e-17 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1257 - 1384 2.3e-14 Ribonuclease-III-like
Ribonuclease_3 PF00636 1278 - 1385 1.3e-19 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 753, 1541, 2136, 4284
AasI GACNNNNNNGTC 1 cut(s) 1515
Acc36I ACCTGC 3 cut(s) 1897, 3333, 3876
AccB1I GGYRCC 3 cut(s) 71, 1949, 2117
AccB7I CCANNNNNTGG 2 cut(s) 1867, 2030
AccI GTMKAC 2 cut(s) 3233, 4185
AccII CGCG 3 cut(s) 2082, 3495, 4322
AclI AACGTT 1 cut(s) 4739
AclWI GGATC 7 cut(s) 916, 1581, 1628, 2528, 3377, 3905, 4775
AcoI YGGCCR 1 cut(s) 2715
AcsI RAATTY 9 cut(s) 28, 395, 1013, 1102, 1302, 1431, 2138, 2336, 2602
AcuI CTGAAG 4 cut(s) 1659, 2894, 3693, 3927
AfiI CCNNNNNNNGG 6 cut(s) 1509, 1867, 2030, 2851, 3715, 4757
AflII CTTAAG 1 cut(s) 448
AflIII ACRYGT 1 cut(s) 2448
AhdI GACNNNNNGTC 1 cut(s) 4646
AjnI CCWGG 4 cut(s) 933, 3403, 3913, 4460
AjuI GAANNNNNNNTTGG 4 cut(s) 751, 783, 3801, 3833
AloI GAACNNNNNNTCC 2 cut(s) 1422, 1454
Alw21I GWGCWC 3 cut(s) 2578, 4161, 4665
AlwI GGATC 7 cut(s) 916, 1581, 1628, 2528, 3377, 3905, 4775
AlwNI CAGNNNCTG 5 cut(s) 182, 2030, 2863, 2948, 4571
Ama87I CYCGRG 1 cut(s) 4644
AoxI GGCC 8 cut(s) 22, 270, 798, 2021, 2715, 2950, 3526, 4088
ApoI RAATTY 9 cut(s) 28, 395, 1013, 1102, 1302, 1431, 2138, 2336, 2602
ArsI GACNNNNNNTTYG 4 cut(s) 870, 902, 1760, 1792
Asp700I GAANNNNTTC 4 cut(s) 846, 1176, 1376, 4314
AspA2I CCTAGG 1 cut(s) 4784
AspLEI GCGC 4 cut(s) 102, 2084, 2627, 3300
AsuC2I CCSGG 1 cut(s) 2853
AsuHPI GGTGA 7 cut(s) 257, 2603, 3227, 3863, 4085, 4319, 4505
AsuII TTCGAA 2 cut(s) 1088, 4592
AvaI CYCGRG 1 cut(s) 4644
AvaII GGWCC 6 cut(s) 886, 1874, 3401, 3865, 4097, 4640
AvrII CCTAGG 1 cut(s) 4784
AxyI CCTNAGG 1 cut(s) 4101
BalI TGGCCA 1 cut(s) 2717
BanI GGYRCC 3 cut(s) 71, 1949, 2117
BanII GRGCYC 3 cut(s) 1516, 4161, 4665
Bbv12I GWGCWC 3 cut(s) 2578, 4161, 4665
BceAI ACGGC 3 cut(s) 609, 2105, 3375
BciT130I CCWGG 4 cut(s) 935, 3405, 3915, 4462
BciVI GTATCC 3 cut(s) 2859, 3442, 4537
BclI TGATCA 3 cut(s) 64, 258, 1960
BcnI CCSGG 1 cut(s) 2853
BfmI CTRYAG 4 cut(s) 690, 3237, 3612, 4815
BfoI RGCGCY 1 cut(s) 103
BfrI CTTAAG 1 cut(s) 448
BfuAI ACCTGC 3 cut(s) 1897, 3333, 3876
BfuI GTATCC 3 cut(s) 2859, 3442, 4537
BlnI CCTAGG 1 cut(s) 4784
BlpI GCTNAGC 1 cut(s) 2577
BmcAI AGTACT 3 cut(s) 1324, 1775, 2816
Bme1390I CCNGG 5 cut(s) 935, 2853, 3405, 3915, 4462
Bme18I GGWCC 6 cut(s) 886, 1874, 3401, 3865, 4097, 4640
BmeRI GACNNNNNGTC 1 cut(s) 4646
BmeT110I CYCGRG 1 cut(s) 4644
BmiI GGNNCC 7 cut(s) 73, 273, 352, 1951, 2119, 3544, 3635
BmrFI CCNGG 5 cut(s) 935, 2853, 3405, 3915, 4462
BmrI ACTGGG 3 cut(s) 1326, 4484, 4601
BmuI ACTGGG 3 cut(s) 1326, 4484, 4601
BpmI CTGGAG 1 cut(s) 1722
Bpu10I CCTNAGC 2 cut(s) 2211, 2994
Bpu1102I GCTNAGC 1 cut(s) 2577
Bpu14I TTCGAA 2 cut(s) 1088, 4592
BpuEI CTTGAG 4 cut(s) 3674, 3951, 4247, 4448
BpuMI CCSGG 1 cut(s) 2853
Bsa29I ATCGAT 1 cut(s) 3067
BsaAI YACGTR 1 cut(s) 3368
BsaBI GATNNNNATC 4 cut(s) 3073, 3728, 3909, 4862
BsaI GGTCTC 4 cut(s) 953, 3418, 3483, 3776
BsaJI CCNNGG 7 cut(s) 354, 943, 1866, 3192, 3663, 3765, 4784
BsaWI WCCGGW 2 cut(s) 515, 2726
BsaXI ACNNNNNCTCC 8 cut(s) 583, 613, 1695, 1725, 1828, 1858, 4104, 4134
Bsc4I CCNNNNNNNGG 6 cut(s) 1509, 1867, 2030, 2851, 3715, 4757
Bse21I CCTNAGG 1 cut(s) 4101
Bse3DI GCAATG 3 cut(s) 1838, 3118, 4006
Bse8I GATNNNNATC 4 cut(s) 3073, 3728, 3909, 4862
BseBI CCWGG 4 cut(s) 935, 3405, 3915, 4462
BseCI ATCGAT 1 cut(s) 3067
BseDI CCNNGG 7 cut(s) 354, 943, 1866, 3192, 3663, 3765, 4784
BseJI GATNNNNATC 4 cut(s) 3073, 3728, 3909, 4862
BseLI CCNNNNNNNGG 6 cut(s) 1509, 1867, 2030, 2851, 3715, 4757
BseMI GCAATG 3 cut(s) 1838, 3118, 4006
BseMII CTCAG 8 cut(s) 2591, 2763, 2802, 2985, 3112, 3184, 3792, 4515
BseRI GAGGAG 2 cut(s) 353, 1439
BseYI CCCAGC 1 cut(s) 2383
BsgI GTGCAG 2 cut(s) 1060, 2055
Bsh1236I CGCG 3 cut(s) 2082, 3495, 4322
BshFI GGCC 8 cut(s) 24, 272, 800, 2023, 2717, 2952, 3528, 4090
BshNI GGYRCC 3 cut(s) 71, 1949, 2117
BshVI ATCGAT 1 cut(s) 3067
BsiHKAI GWGCWC 3 cut(s) 2578, 4161, 4665
BsiHKCI CYCGRG 1 cut(s) 4644
BsiSI CCGG 4 cut(s) 516, 1877, 2727, 2852
BslFI GGGAC 3 cut(s) 2080, 2647, 4381
BslI CCNNNNNNNGG 6 cut(s) 1509, 1867, 2030, 2851, 3715, 4757
BsmBI CGTCTC 1 cut(s) 3018
BsmFI GGGAC 3 cut(s) 2080, 2647, 4381
BsmI GAATGC 5 cut(s) 458, 994, 1699, 2076, 4775
BsnI GGCC 8 cut(s) 24, 272, 800, 2023, 2717, 2952, 3528, 4090
Bso31I GGTCTC 4 cut(s) 953, 3418, 3483, 3776
BsoBI CYCGRG 1 cut(s) 4644
Bsp119I TTCGAA 2 cut(s) 1088, 4592
Bsp1286I GDGCHC 4 cut(s) 1516, 2578, 4161, 4665
Bsp1407I TGTACA 3 cut(s) 1661, 4333, 4743
Bsp1720I GCTNAGC 1 cut(s) 2577
Bsp19I CCATGG 1 cut(s) 3192
BspANI GGCC 8 cut(s) 24, 272, 800, 2023, 2717, 2952, 3528, 4090
BspCNI CTCAG 8 cut(s) 2590, 2764, 2803, 2986, 3111, 3183, 3793, 4516
BspDI ATCGAT 1 cut(s) 3067
BspFNI CGCG 3 cut(s) 2082, 3495, 4322
BspLI GGNNCC 7 cut(s) 73, 273, 352, 1951, 2119, 3544, 3635
BspMAI CTGCAG 2 cut(s) 3616, 4819
BspMI ACCTGC 3 cut(s) 1897, 3333, 3876
BspPI GGATC 7 cut(s) 916, 1581, 1628, 2528, 3377, 3905, 4775
BspT104I TTCGAA 2 cut(s) 1088, 4592
BspT107I GGYRCC 3 cut(s) 71, 1949, 2117
BspTI CTTAAG 1 cut(s) 448
BspTNI GGTCTC 4 cut(s) 953, 3418, 3483, 3776
BsrDI GCAATG 3 cut(s) 1838, 3118, 4006
BsrGI TGTACA 3 cut(s) 1661, 4333, 4743
BssECI CCNNGG 7 cut(s) 354, 943, 1866, 3192, 3663, 3765, 4784
BssNAI GTATAC 2 cut(s) 3234, 4186
BssT1I CCWWGG 7 cut(s) 354, 943, 1866, 3192, 3663, 3765, 4784
Bst1107I GTATAC 2 cut(s) 3234, 4186
Bst2UI CCWGG 4 cut(s) 935, 3405, 3915, 4462
Bst6I CTCTTC 7 cut(s) 143, 999, 3016, 3099, 3257, 4460, 4793
BstAFI CTTAAG 1 cut(s) 448
BstAUI TGTACA 3 cut(s) 1661, 4333, 4743
BstBAI YACGTR 1 cut(s) 3368
BstBI TTCGAA 2 cut(s) 1088, 4592
BstC8I GCNNGC 7 cut(s) 1882, 2025, 2208, 3091, 3795, 3936, 4566
BstDSI CCRYGG 1 cut(s) 3192
BstFNI CGCG 3 cut(s) 2082, 3495, 4322
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 4 cut(s) 102, 2084, 2627, 3300
BstNI CCWGG 4 cut(s) 935, 3405, 3915, 4462
BstNSI RCATGY 3 cut(s) 1884, 2452, 3797
BstSCI CCNGG 5 cut(s) 933, 2851, 3403, 3913, 4460
BstSFI CTRYAG 4 cut(s) 690, 3237, 3612, 4815
BstUI CGCG 3 cut(s) 2082, 3495, 4322
BstX2I RGATCY 3 cut(s) 921, 1633, 4780
BstXI CCANNNNNNTGG 2 cut(s) 1196, 3393
BstYI RGATCY 3 cut(s) 921, 1633, 4780
BstZ17I GTATAC 2 cut(s) 3234, 4186
Bsu15I ATCGAT 1 cut(s) 3067
Bsu36I CCTNAGG 1 cut(s) 4101
BsuI GTATCC 3 cut(s) 2859, 3442, 4537
BsuRI GGCC 8 cut(s) 24, 272, 800, 2023, 2717, 2952, 3528, 4090
BsuTUI ATCGAT 1 cut(s) 3067
BtgI CCRYGG 1 cut(s) 3192
BtsI GCAGTG 2 cut(s) 1043, 1245
BveI ACCTGC 3 cut(s) 1897, 3333, 3876
Cac8I GCNNGC 7 cut(s) 1882, 2025, 2208, 3091, 3795, 3936, 4566
CaiI CAGNNNCTG 5 cut(s) 182, 2030, 2863, 2948, 4571
CfoI GCGC 4 cut(s) 102, 2084, 2627, 3300
ClaI ATCGAT 1 cut(s) 3067
CpoI CGGWCCG 1 cut(s) 1874
CseI GACGC 1 cut(s) 2245
CspI CGGWCCG 1 cut(s) 1874
DraI TTTAAA 3 cut(s) 331, 400, 831
DrdI GACNNNNNNGTC 1 cut(s) 1515
DriI GACNNNNNGTC 1 cut(s) 4646
DseDI GACNNNNNNGTC 1 cut(s) 1515
EaeI YGGCCR 1 cut(s) 2715
Eam1104I CTCTTC 7 cut(s) 143, 999, 3016, 3099, 3257, 4460, 4793
Eam1105I GACNNNNNGTC 1 cut(s) 4646
EarI CTCTTC 7 cut(s) 143, 999, 3016, 3099, 3257, 4460, 4793
Ecl136II GAGCTC 2 cut(s) 4159, 4663
Eco130I CCWWGG 7 cut(s) 354, 943, 1866, 3192, 3663, 3765, 4784
Eco147I AGGCCT 1 cut(s) 800
Eco24I GRGCYC 3 cut(s) 1516, 4161, 4665
Eco31I GGTCTC 4 cut(s) 953, 3418, 3483, 3776
Eco32I GATATC 2 cut(s) 2002, 3135
Eco47I GGWCC 6 cut(s) 886, 1874, 3401, 3865, 4097, 4640
Eco53kI GAGCTC 2 cut(s) 4159, 4663
Eco57I CTGAAG 4 cut(s) 1659, 2894, 3693, 3927
Eco81I CCTNAGG 1 cut(s) 4101
Eco88I CYCGRG 1 cut(s) 4644
EcoICRI GAGCTC 2 cut(s) 4159, 4663
EcoO109I RGGNCCY 3 cut(s) 886, 4097, 4640
EcoRI GAATTC 3 cut(s) 28, 1431, 2602
EcoRII CCWGG 4 cut(s) 933, 3403, 3913, 4460
EcoRV GATATC 2 cut(s) 2002, 3135
EcoT14I CCWWGG 7 cut(s) 354, 943, 1866, 3192, 3663, 3765, 4784
EcoT22I ATGCAT 4 cut(s) 6, 2566, 3799, 4533
EcoT38I GRGCYC 3 cut(s) 1516, 4161, 4665
ErhI CCWWGG 7 cut(s) 354, 943, 1866, 3192, 3663, 3765, 4784
Esp3I CGTCTC 1 cut(s) 3018
FalI AAGNNNNNCTT 4 cut(s) 446, 478, 3758, 3790
FaqI GGGAC 3 cut(s) 2080, 2647, 4381
FauNDI CATATG 2 cut(s) 953, 2985
FbaI TGATCA 3 cut(s) 64, 258, 1960
FblI GTMKAC 2 cut(s) 3233, 4185
FriOI GRGCYC 3 cut(s) 1516, 4161, 4665
GlaI GCGC 4 cut(s) 101, 2083, 2626, 3299
GsaI CCCAGC 1 cut(s) 2387
GsuI CTGGAG 1 cut(s) 1722
HaeII RGCGCY 1 cut(s) 103
HaeIII GGCC 8 cut(s) 24, 272, 800, 2023, 2717, 2952, 3528, 4090
HapII CCGG 4 cut(s) 516, 1877, 2727, 2852
HgaI GACGC 1 cut(s) 2245
HhaI GCGC 4 cut(s) 102, 2084, 2627, 3300
Hin6I GCGC 4 cut(s) 100, 2082, 2625, 3298
HinP1I GCGC 4 cut(s) 100, 2082, 2625, 3298
HincII GTYRAC 2 cut(s) 3924, 3949
HindII GTYRAC 2 cut(s) 3924, 3949
HindIII AAGCTT 3 cut(s) 1923, 2265, 3743
HpaI GTTAAC 2 cut(s) 3924, 3949
HpaII CCGG 4 cut(s) 516, 1877, 2727, 2852
HphI GGTGA 7 cut(s) 257, 2603, 3227, 3863, 4085, 4319, 4505
HpyCH4IV ACGT 5 cut(s) 1462, 2971, 3027, 3367, 4739
HpySE526I ACGT 5 cut(s) 1462, 2971, 3027, 3367, 4739
HspAI GCGC 4 cut(s) 100, 2082, 2625, 3298
Ksp22I TGATCA 3 cut(s) 64, 258, 1960
KspAI GTTAAC 2 cut(s) 3924, 3949
MaeII ACGT 5 cut(s) 1462, 2971, 3027, 3367, 4739
MfeI CAATTG 1 cut(s) 1943
MflI RGATCY 3 cut(s) 921, 1633, 4780
MhlI GDGCHC 4 cut(s) 1516, 2578, 4161, 4665
MlsI TGGCCA 1 cut(s) 2717
MluNI TGGCCA 1 cut(s) 2717
MlyI GAGTC 5 cut(s) 2207, 2785, 3746, 4656, 4805
MmeI TCCRAC 2 cut(s) 3487, 3843
Mox20I TGGCCA 1 cut(s) 2717
Mph1103I ATGCAT 4 cut(s) 6, 2566, 3799, 4533
MroXI GAANNNNTTC 4 cut(s) 846, 1176, 1376, 4314
MscI TGGCCA 1 cut(s) 2717
MslI CAYNNNNRTG 4 cut(s) 1229, 1541, 3172, 4068
Msp20I TGGCCA 1 cut(s) 2717
MspA1I CMGCKG 3 cut(s) 2387, 2771, 4339
MspCI CTTAAG 1 cut(s) 448
MspI CCGG 4 cut(s) 516, 1877, 2727, 2852
MspR9I CCNGG 5 cut(s) 935, 2853, 3405, 3915, 4462
MunI CAATTG 1 cut(s) 1943
Mva1269I GAATGC 5 cut(s) 458, 994, 1699, 2076, 4775
MvaI CCWGG 4 cut(s) 935, 3405, 3915, 4462
MvnI CGCG 3 cut(s) 2082, 3495, 4322
NciI CCSGG 1 cut(s) 2853
NcoI CCATGG 1 cut(s) 3192
NdeI CATATG 2 cut(s) 953, 2985
NlaIV GGNNCC 7 cut(s) 73, 273, 352, 1951, 2119, 3544, 3635
NmuCI GTSAC 7 cut(s) 245, 1243, 1751, 2759, 3518, 4073, 4210
NsiI ATGCAT 4 cut(s) 6, 2566, 3799, 4533
NspI RCATGY 3 cut(s) 1884, 2452, 3797
NspV TTCGAA 2 cut(s) 1088, 4592
PaeI GCATGC 2 cut(s) 1884, 3797
PaeR7I CTCGAG 1 cut(s) 4644
PceI AGGCCT 1 cut(s) 800
PciI ACATGT 1 cut(s) 2448
PctI GAATGC 5 cut(s) 458, 994, 1699, 2076, 4775
PdmI GAANNNNTTC 4 cut(s) 846, 1176, 1376, 4314
PflMI CCANNNNNTGG 2 cut(s) 1867, 2030
PfoI TCCNGGA 1 cut(s) 3403
PleI GAGTC 5 cut(s) 2206, 2784, 3745, 4655, 4805
PpsI GAGTC 5 cut(s) 2206, 2784, 3745, 4655, 4805
Ppu21I YACGTR 1 cut(s) 3368
PpuMI RGGWCCY 3 cut(s) 886, 4097, 4640
PscI ACATGT 1 cut(s) 2448
PsiI TTATAA 4 cut(s) 753, 1541, 2136, 4284
Psp124BI GAGCTC 2 cut(s) 4161, 4665
Psp1406I AACGTT 1 cut(s) 4739
Psp5II RGGWCCY 3 cut(s) 886, 4097, 4640
Psp6I CCWGG 4 cut(s) 933, 3403, 3913, 4460
PspFI CCCAGC 1 cut(s) 2383
PspGI CCWGG 4 cut(s) 933, 3403, 3913, 4460
PspN4I GGNNCC 7 cut(s) 73, 273, 352, 1951, 2119, 3544, 3635
PspPPI RGGWCCY 3 cut(s) 886, 4097, 4640
PspXI VCTCGAGB 1 cut(s) 4644
PstI CTGCAG 2 cut(s) 3616, 4819
PstNI CAGNNNCTG 5 cut(s) 182, 2030, 2863, 2948, 4571
PsuI RGATCY 3 cut(s) 921, 1633, 4780
PvuII CAGCTG 2 cut(s) 2387, 4339
RseI CAYNNNNRTG 4 cut(s) 1229, 1541, 3172, 4068
Rsr2I CGGWCCG 1 cut(s) 1874
RsrII CGGWCCG 1 cut(s) 1874
SacI GAGCTC 2 cut(s) 4161, 4665
ScaI AGTACT 3 cut(s) 1324, 1775, 2816
SchI GAGTC 5 cut(s) 2207, 2785, 3746, 4656, 4805
ScrFI CCNGG 5 cut(s) 935, 2853, 3405, 3915, 4462
SduI GDGCHC 4 cut(s) 1516, 2578, 4161, 4665
SfcI CTRYAG 4 cut(s) 690, 3237, 3612, 4815
Sfr274I CTCGAG 1 cut(s) 4644
SfuI TTCGAA 2 cut(s) 1088, 4592
SinI GGWCC 6 cut(s) 886, 1874, 3401, 3865, 4097, 4640
SlaI CTCGAG 1 cut(s) 4644
SmiMI CAYNNNNRTG 4 cut(s) 1229, 1541, 3172, 4068
SmlI CTYRAG 6 cut(s) 448, 3653, 3966, 4226, 4427, 4644
SmoI CTYRAG 6 cut(s) 448, 3653, 3966, 4226, 4427, 4644
SphI GCATGC 2 cut(s) 1884, 3797
SseBI AGGCCT 1 cut(s) 800
SspI AATATT 1 cut(s) 2227
SstI GAGCTC 2 cut(s) 4161, 4665
StuI AGGCCT 1 cut(s) 800
StyD4I CCNGG 5 cut(s) 933, 2851, 3403, 3913, 4460
StyI CCWWGG 7 cut(s) 354, 943, 1866, 3192, 3663, 3765, 4784
TaiI ACGT 5 cut(s) 1465, 2974, 3030, 3370, 4742
TaqII GACCGA 1 cut(s) 1862
TauI GCSGC 5 cut(s) 77, 80, 1856, 2872, 3941
TseFI GTSAC 7 cut(s) 245, 1243, 1751, 2759, 3518, 4073, 4210
Tsp45I GTSAC 7 cut(s) 245, 1243, 1751, 2759, 3518, 4073, 4210
TspGWI ACGGA 2 cut(s) 1721, 4169
Van91I CCANNNNNTGG 2 cut(s) 1867, 2030
Vha464I CTTAAG 1 cut(s) 448
VpaK11BI GGWCC 6 cut(s) 886, 1874, 3401, 3865, 4097, 4640
XapI RAATTY 9 cut(s) 28, 395, 1013, 1102, 1302, 1431, 2138, 2336, 2602
XbaI TCTAGA 2 cut(s) 982, 3151
XceI RCATGY 3 cut(s) 1884, 2452, 3797
XcmI CCANNNNNNNNNTGG 2 cut(s) 2828, 3199
XhoI CTCGAG 1 cut(s) 4644
XmaJI CCTAGG 1 cut(s) 4784
XmiI GTMKAC 2 cut(s) 3233, 4185
XmnI GAANNNNTTC 4 cut(s) 846, 1176, 1376, 4314
ZrmI AGTACT 3 cut(s) 1324, 1775, 2816
Zsp2I ATGCAT 4 cut(s) 6, 2566, 3799, 4533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.