Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0001909

Physical Location & Seq

Genomic Coordinates Forward (+)

46773910 .. 46782600

Gene Structure 8691 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G023980.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4773 bp

ATGAGTTCAGGTATCACATTGATAACTTTGAAAGTTCAGGTATCATTTCAAAAGTCCTCGAAGAGTTCAGACACTGTTGAATGTGTTTGCACTGTGTACAGGTATCAAGTGAAAGTGTTCGAAGTCGCAAAGCGTAAGAACATAATTGCAGTGTTGGAAACTGGTGCTGGGAAAACTATGATTGCTGTGATGCTCATCAAACACATTGCTGATCAACACATCATCAAGTCCATTGGCCACAAGAAGTTGATAATTTTCATGGCCCCAACAGTTCATCTTGTCATTCAGCAATATGAGGTTATAAAAAAGTCTACAACTCTTGAAGTGGGAGTATACTACGGAGCTAAGGGCGTTGATGACTGGAGTCCAGAATATTGGGAGGTGGAAGTGAATACACATAATATAATGGTCATGACACCTCAAATTCTCCTGGATGCTTTGCGGAATGCATTTTTGAAGATAGAGATGATTTGCTTGATGGTCTTTGATGAATGCCACCGTGCCACTGGCAATCATCCCTACACGAAAATAATCAAGGAATTTCATCACAAATCAGGAAACAAGCCAAAGATTTTTGGAATGACAGCATCACCAGTCATTAAAAAAGGTGTCTCATCCGCCATGGACTGTGAGGATCAGATATCACAACTTGAAAGCATCTTGGATTCCCAGGTTTATACAATAGAAGACAGGACAGAGATTGAAGAGCATGTTCCCTCTGCAGTCCAAATTTGTAAATTTTATGACCCGACATGGTTGATCTGTGTGGATTTGAAAGCAAAAATGGAAGCTTTATGGTCAAAGTTTGATGCTTCTATCATGTCAAAGTTGCAAGGGCCAGAGAAAAGCGAGTATGAAGACATTAATGATAAAATCAAGATGCTAAGAAAGCGGTTGTCCAATGACTACATGAAAATTTTGTATTGCCTTGATGATCTTGGTATCGTATGCGCATATGAGGCTGTGAAAGTCTGTCTGGAGAATGCCCCTGATGTCAAAGAGGAGTGCACATTTTTTAGAGAAAGCTCTTTGCAATGCAGATATTTTCTTGATGAAGTCTTAAATATGATCAGTGAATTTCTTCAAAAAGGTTATATACTTCATACAGATCTTGAGTCAGACTATATAAAGGCACACAGAGTGGGATACATATCTCCGAAGTTACATGAACTTCTTCAAATTTTCCACTCATTTGAGAGGGATAGACAAGTTCAGTGCCTTATTTTCGTAGAGAGAATTGTCACAGCCAAAGTAATGCAAAGATATGTGAAGAAACTGCCATGTTTATCTCATTTCACAGCTTCATATTTGACCGGAAGTACCACATCAGTTGATGCACTTGCACCGAAATTGCAGAAGGAAATTTTGGAGTCATTTTACAGTGGGAAGGTCAATCTACTATTTGCCACTGATGTAGTTGAGGAGGGAATTCATGTCCAGAACTGCTCCTGTGTGATCCGGTTTGATCTTCCCAAGACGGTTCGTAGCTATGTTCAGTCTCGGGGACGAGCACGAAAATATGATTCCCAATATATCATAATGCTTGAAAGGGACAACAAGAAGCAAAGAGACCATCTCTTTGACATAATCAGGAGTGAGTACTCAATGACAGATACATCGAGAAATCGAGATCCTGATGGATGCAATTTAAGAGCTTGTAACTTTGAAGATGCAAATGCATACTATGTAGAAGCTACTGGAGCATCAGTTACTCCAGACTCTAGTGTCAGTCTCATATATCGGTACTGTGAAAAGCTCCCTGGAGATAGGTACTTTATTCCTAGGCCAAATTTTCACTTCAAACTATTTGAAGGGTTGTATCAGTGTGAAATAACATTGCCTCCAAATGCGGCATTTCAAACACTAGTTGTTCCTATGAGTAGAAACGGTCATTCATCAAAGCAGCTTGTAAGCTTGGAAGCTTGTAAAATGCTGCATCAAATGGGTGCTTTGGATGATCATCTTCTCCCACTTGTCGAAGTGCCTCTTGATAATGAAGTGATTGTGAAAAGCAAAGAATTATCTGCAGGTGCTGGAACTACAAAAAGGAAAGAGTTACATGGGATGACTTGCATTCGTGCATTAAGTGGAACTTGGAGAGAGAAAGTTGATGGTGCCAGTTTTGAGGCCTACAAATTTGAGTTCTCCTGTAATGTTGATGAGACATATTCAGGATTTGTACTACTAGTCGAGATGAAACTTGATGATGACGTGGGAAATGCTGAAGTGGATCTCTACTTAATTGCAAAGATGGTCAAAGCTTACGTTTCTTCATGTGGAAAGGTGCATCTCAGTGCAGATCAGATAAAGGAAGCCATGTGCTTTCAGGAATTTTTCTTTAATGGTTTGTTTGGGAGATTGTTTCTTGGATCCGAAGCAAAAGGCACAGAAAGAAAATTTCTACTTCAGACTGAAATAAAATCACTATGGTGCTCATCGTACAGCTATTTACTTTTACCGACTGAGGCATCTGACAGTTCAAATAAGAAATCTTGGAACATAGATTGGATGGGAATCTGTTCCTGTGCTAATGTGGTGGAGTTTCTTAGGAAACACTATTCCAATGGTCTTGAGAATTTCAAAGGCGGTAGAGGAAATATATTACTTTCTAGGACTGGTTCATCCATGACAGATTGCAAGGCTGAAAATTTAGTCCAGTTTGCTAATAGTTCAATTGATGTCAATGATCTTGAAGATATGGTAGTGTTGGCTATTCACACAGGAAGAATCTACTCTGTTATTGAAGTTGTTAGTAGCATTTCTGCTGAGAGTCCCTTGGAAGAAAATGCTGATGCTGCCTCATCAGATTATACTTATGTTGAGTACTTCAAAACAACGTATGGGATTGTACTGAAGTATCCTAGACATCCTCTATTGCGGTTGAAGCAAAGTCATAATGCACATAACCTGCTTGTTGATCAAGGTGTGTCATCACCAGACGGTCTAGTTCAAGAAAAGCAACAAGCTCATGTTCATATGCCACCAGAAATCTTAGTCAGTATTGATGTTCGAGTTGACGTTCTAAAATCATTTTATTTACTACCATCACTAATGTATCGTCTGGAGTCCCTAATGTTAGCCAGCCAGATCAGGAAAGAGATCAATGGCCAGTCTAGCAACTTTTATATATCAAGCTCATTGATTCTAGAAGCACTCACAACACTTAGATGCTGTGAAAATTTTTCACTGGAGCGTTTGGAGTTGCTTGGAGATTCAGTTCTCAAATATGCTGTGAGCTCCTACCTCTTTCTTAAATATCCCGAGAAACATGAAGGACAATTAACTGCTCGACGAACAAGAGCTATTTGCAACTCAAACCTGCACAAACATGGAACGAAAAACAAGTTACAGGGATACATACGAGACAGTGCATTTGAACCACGACGTTGGGCTGCTCCAGGGCAGCTTTCTAGATTCCCAGATCCATGTCAATGCGGAGTGGATACTTTGGAAGTGCCCTTGGACAGTAGATTTCAAACTGAAAAGGCTGTTAAGGTTGGAAAATTCTGTGATAAAGGTCACAGATGGATGAACTCAAAAACCATTGCTGATTGTGTTGAGGCCCTCATAGGAGCTTATTATGTTGGTGGAGGATTGTTTGCTGCACTTCATTTGATGAAGTGGCTCAGCATTGATTCTGAAACCGGACCCTCATTTGCTGATGAAGCCATTACAAGGGCATCACTTCGATCCTACAATCCCAAAACTAATGAACTTGCTAACTTGGAGTTGAAGCTTAGCTATGAATTTTCAGTCAAGGGCTTGTTACATGAGGCCATCACACATGCTTCTCAGCAAAAACTAGGTGCCAGCTACTGTTATCAGAGGCTTGAATTTCTTGGAGACTCTGTGTTGGATCTGCTAACTACACAGCATCTCTATCGCAGCCACACAGATATTGATCCTGGAGTGTTGACTGATTTGCGGTCAGCTGCTGTTAGTAATGAAAACTTTGCTCAAGCTGCTATTAGACAAAACCTTCATCCACATCTTCAACATTGCTCTGGGTTACTTCGGAGTCACGTAACCGAGTATGAGAGGTTGCTTACAGAAGCTGAGCACAGTACCAGCTCAGTGGAAGACATTAAGTGTCCAAAAGCTCTTGGTGACATGGTGGAAAGTATAGCAGGAGCTATACTAATTGATACAAAGCTTAATCTTGATGAAGTATGGAGGGTTTTTAAACCAATATTGTCTCCAATTGTGACCCCTTCTAGTCTTCAATTGCATCCGCTACGTAAACTGTCTGAATTATGTGATTCACTTGGGTACTTTGTGAAAGAAACATGTACTAAAGATGATACACTAGTAAATGTGGAACTAAGTTTGCAGCTGGAAGATGTTCTTTTGGTTGGAAACGGCTTAGACCGGAGTAGAAAAGCTGCGAAACATAAGGCAGCTCGTCAAGTGTTGAAGGCGCTCGAGGTTAGTTGTTATGGTACTTCTCTTCTAATTATTGGTCCAATCGATACGAAAAAAGGTGAACCTCGAAAATCTCTCTTTGAGCTATGTAAGAAATTGCAGTGGCCAATGCCTGAATTCACAACAACAGAGCAGAAGTCAAGAACTCCAATTGAATTCGGTGAGGGTTCTGAAAAGAGAATAGGTTTCAGCAGCTTTCAATCAAAAATCAGCTTGCACATACCCAACTCTTGCACTATTGTATGCACAGGAGATCCTAGAGCCGATAAGAAGACTTCCATGGATTCTGCAGTTGTTTCCATGCTTCACGAGCTTCAACGACGGAGAAAACTTGTCATTTTCCAGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1590

Amino Acids

179.67

Weight (kDa)

6.69

Isoelectric Point (pI)

41.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	34 - 198	4.5e-18	DEAD/DEAH box helicase
ResIII	PF04851	43 - 198	2.5e-16	Type III restriction enzyme, res subunit
Helicase_C	PF00271	387 - 504	4e-18	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	575 - 659	2.1e-21	Dicer dimerisation domain
PAZ	PF02170	894 - 1013	8e-10	PAZ domain
Ribonuclease_3	PF00636	1064 - 1197	5.2e-21	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1064 - 1121	1e-06	Ribonuclease-III-like
Ribonucleas_3_3	PF14622	1254 - 1382	1.2e-13	Ribonuclease-III-like
Ribonuclease_3	PF00636	1275 - 1383	4.1e-20	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001909)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920
fragaria_vesca	FvH4_2g07790 FvH4_2g18930 FvH4_2g18930 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780
malus_domestica	MD08G1046300.v1.1 MD10G1163700.v1.1 MD15G1047500.v1.1
prunus_persica	Prupe.1G401900_v2.0.a1 Prupe.1G401900_v2.0.a1 Prupe.8G202000_v2.0.a1 Prupe.8G202000_v2.0.a1
pyrus_communis	pycom08g03830 pycom08g03840 pycom15g04670
rosa_chinensis	RchiOBHm_Chr6g0284721 RchiOBHm_Chr6g0312121
rosa_laevigata	RLG00000010353 RLG00000012724
rosa_multiflora	Rmu_sc0004065.1_g000007 Rmu_sc0007474.1_g000021
rosa_roxburghii	Rroxscaffold_7G00157050 Rroxscaffold_7G00184440
rosa_rugosa	Rorug06G0165900 Rorug06G0392600 Rorug06G0392700 Rorug06G0392800
rosa_samantha	Rh4CG118000 Rh6AG279000 Rh6AG509700 Rh6BG279000 Rh6BG520600 Rh6CG281300 Rh6CG526600 Rh6DG274000 Rh6DG512000
rosa_wichuraiana	Rw6G023980 Rw6G044350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	302
AarI	CACCTGC	1 cut(s)	2018
AasI	GACNNNNNNGTC	1 cut(s)	1724
Acc16I	TGCGCA	1 cut(s)	952
Acc36I	ACCTGC	3 cut(s)	2018, 2916, 3327
AccB1I	GGYRCC	2 cut(s)	2114, 3806
AccI	GTMKAC	2 cut(s)	311, 333
AciI	CCGC	9 cut(s)	442, 618, 892, 1850, 2586, 2878, 3435, 3925, 4232
AcoI	YGGCCR	3 cut(s)	235, 3106, 4526
AcuI	CTGAAG	3 cut(s)	2244, 2390, 2873
AdeI	CACNNNGTG	1 cut(s)	1141
AfiI	CCNNNNNNNGG	1 cut(s)	3675
AflIII	ACRYGT	1 cut(s)	4286
AhlI	ACTAGT	3 cut(s)	1864, 2185, 4306
AjiI	CACGTC	1 cut(s)	2212
AjnI	CCWGG	5 cut(s)	429, 669, 1759, 3397, 3904
AjuI	GAANNNNNNNTTGG	2 cut(s)	1349, 1381
AleI	CACNNNNGTG	1 cut(s)	2428
AloI	GAACNNNNNNTCC	2 cut(s)	3201, 3233
Alw21I	GWGCWC	5 cut(s)	1010, 1513, 2435, 3239, 4062
Alw26I	GTCTC	8 cut(s)	616, 1503, 1563, 1736, 2156, 3357, 3837, 4200
Alw44I	GTGCAC	1 cut(s)	1006
AlwNI	CAGNNNCTG	5 cut(s)	74, 2033, 3816, 3935, 4055
Ama87I	CYCGRG	3 cut(s)	1500, 3260, 4421
AoxI	GGCC	9 cut(s)	235, 261, 836, 1784, 2127, 3106, 3561, 3774, 4526
ApaLI	GTGCAC	1 cut(s)	1006
ArsI	GACNNNNNNTTYG	4 cut(s)	1562, 1594, 3356, 3388
AseI	ATTAAT	1 cut(s)	864
Asp700I	GAANNNNTTC	5 cut(s)	116, 1080, 1173, 2518, 4341
AspA2I	CCTAGG	1 cut(s)	1781
AspLEI	GCGC	2 cut(s)	953, 4420
AspS9I	GGNCC	5 cut(s)	262, 836, 3562, 3647, 4460
AsuHPI	GGTGA	5 cut(s)	582, 2925, 4118, 4493, 4595
AsuII	TTCGAA	1 cut(s)	120
AvaI	CYCGRG	3 cut(s)	1500, 3260, 4421
AvaII	GGWCC	2 cut(s)	3647, 4460
AvrII	CCTAGG	1 cut(s)	1781
BaeGI	GKGCMC	2 cut(s)	1010, 3459
BaeI	ACNNNNGTAYC	6 cut(s)	2840, 2873, 3038, 3071, 4293, 4326
BalI	TGGCCA	3 cut(s)	237, 3108, 4528
BamHI	GGATCC	1 cut(s)	2369
BanI	GGYRCC	2 cut(s)	2114, 3806
BanII	GRGCYC	1 cut(s)	3239
BauI	CACGAG	1 cut(s)	4730
BbsI	GAAGAC	5 cut(s)	693, 864, 4086, 4211, 4700
Bbv12I	GWGCWC	5 cut(s)	1010, 1513, 2435, 3239, 4062
BccI	CCATC	9 cut(s)	472, 1581, 1631, 2105, 2245, 2503, 3052, 3519, 3785
BceAI	ACGGC	1 cut(s)	4375
BcgI	CGANNNNNNTGC	2 cut(s)	3863, 3897
BciT130I	CCWGG	5 cut(s)	431, 671, 1761, 3399, 3906
BciVI	GTATCC	4 cut(s)	1139, 2868, 3347, 3436
BclI	TGATCA	4 cut(s)	211, 1068, 1957, 2917
BcoDI	GTCTC	8 cut(s)	616, 1503, 1563, 1736, 2156, 3357, 3837, 4200
BcuI	ACTAGT	3 cut(s)	1864, 2185, 4306
BfmI	CTRYAG	3 cut(s)	720, 2025, 4710
BfoI	RGCGCY	1 cut(s)	4421
BfuAI	ACCTGC	3 cut(s)	2018, 2916, 3327
BfuI	GTATCC	4 cut(s)	1139, 2868, 3347, 3436
BglII	AGATCT	1 cut(s)	1108
BlnI	CCTAGG	1 cut(s)	1781
BlpI	GCTNAGC	3 cut(s)	3626, 3737, 4056
BmcAI	AGTACT	2 cut(s)	1601, 2825
Bme1390I	CCNGG	5 cut(s)	431, 671, 1761, 3399, 3906
Bme18I	GGWCC	2 cut(s)	3647, 4460
BmeT110I	CYCGRG	3 cut(s)	1500, 3260, 4421
BmgBI	CACGTC	1 cut(s)	2212
BmgT120I	GGNCC	5 cut(s)	262, 836, 3562, 3647, 4460
BmiI	GGNNCC	5 cut(s)	264, 2116, 2371, 3649, 3808
BmrFI	CCNGG	5 cut(s)	431, 671, 1761, 3399, 3906
BoxI	GACNNNNGTC	1 cut(s)	363
BpiI	GAAGAC	5 cut(s)	693, 864, 4086, 4211, 4700
BplI	GAGNNNNNCTC	2 cut(s)	1560, 1592
BpmI	CTGGAG	9 cut(s)	382, 998, 1698, 1719, 1782, 3083, 3209, 3381, 3927
Bpu10I	CCTNAGC	1 cut(s)	345
Bpu1102I	GCTNAGC	3 cut(s)	3626, 3737, 4056
Bpu14I	TTCGAA	1 cut(s)	120
BpuEI	CTTGAG	3 cut(s)	1133, 2591, 3942
Bsa29I	ATCGAT	1 cut(s)	4467
BsaAI	YACGTR	2 cut(s)	4024, 4238
BsaBI	GATNNNNATC	5 cut(s)	194, 1150, 1959, 2513, 3900
BsaI	GGTCTC	1 cut(s)	1563
BsaJI	CCNNGG	8 cut(s)	621, 669, 1759, 1781, 2775, 3398, 3459, 4701
BsaWI	WCCGGW	4 cut(s)	1313, 1458, 3644, 4368
BsaXI	ACNNNNNCTCC	8 cut(s)	1692, 1722, 1825, 1855, 3201, 3231, 3834, 3864
Bsc4I	CCNNNNNNNGG	1 cut(s)	3675
Bse3DI	GCAATG	5 cut(s)	204, 1040, 1835, 3543, 3997
Bse8I	GATNNNNATC	5 cut(s)	194, 1150, 1959, 2513, 3900
BseBI	CCWGG	5 cut(s)	431, 671, 1761, 3399, 3906
BseCI	ATCGAT	1 cut(s)	4467
BseDI	CCNNGG	8 cut(s)	621, 669, 1759, 1781, 2775, 3398, 3459, 4701
BseJI	GATNNNNATC	5 cut(s)	194, 1150, 1959, 2513, 3900
BseLI	CCNNNNNNNGG	1 cut(s)	3675
BseMI	GCAATG	5 cut(s)	204, 1040, 1835, 3543, 3997
BseMII	CTCAG	7 cut(s)	2305, 2454, 2757, 3640, 3806, 4047, 4086
BseRI	GAGGAG	2 cut(s)	1016, 1436
BseSI	GKGCMC	2 cut(s)	1010, 3459
BseYI	CCCAGC	1 cut(s)	167
BsgI	GTGCAG	3 cut(s)	2316, 3305, 3588
BshFI	GGCC	9 cut(s)	237, 263, 838, 1786, 2129, 3108, 3563, 3776, 4528
BshNI	GGYRCC	2 cut(s)	2114, 3806
BshVI	ATCGAT	1 cut(s)	4467
BsiHKAI	GWGCWC	5 cut(s)	1010, 1513, 2435, 3239, 4062
BsiHKCI	CYCGRG	3 cut(s)	1500, 3260, 4421
BsiSI	CCGG	4 cut(s)	1314, 1459, 3645, 4369
BslFI	GGGAC	4 cut(s)	1518, 1565, 2757, 3052
BslI	CCNNNNNNNGG	1 cut(s)	3675
BsmAI	GTCTC	8 cut(s)	616, 1503, 1563, 1736, 2156, 3357, 3837, 4200
BsmFI	GGGAC	4 cut(s)	1518, 1565, 2757, 3052
BsmI	GAATGC	4 cut(s)	451, 497, 988, 2073
BsnI	GGCC	9 cut(s)	237, 263, 838, 1786, 2129, 3108, 3563, 3776, 4528
Bso31I	GGTCTC	1 cut(s)	1563
BsoBI	CYCGRG	3 cut(s)	1500, 3260, 4421
Bsp119I	TTCGAA	1 cut(s)	120
Bsp1286I	GDGCHC	6 cut(s)	1010, 1513, 2435, 3239, 3459, 4062
Bsp1407I	TGTACA	1 cut(s)	96
Bsp1720I	GCTNAGC	3 cut(s)	3626, 3737, 4056
Bsp19I	CCATGG	2 cut(s)	621, 4701
BspACI	CCGC	9 cut(s)	442, 618, 892, 1850, 2586, 2878, 3435, 3925, 4232
BspANI	GGCC	9 cut(s)	237, 263, 838, 1786, 2129, 3108, 3563, 3776, 4528
BspCNI	CTCAG	7 cut(s)	2304, 2455, 2758, 3639, 3805, 4048, 4085
BspDI	ATCGAT	1 cut(s)	4467
BspHI	TCATGA	1 cut(s)	411
BspLI	GGNNCC	5 cut(s)	264, 2116, 2371, 3649, 3808
BspMAI	CTGCAG	3 cut(s)	724, 2029, 4714
BspMI	ACCTGC	3 cut(s)	2018, 2916, 3327
BspQI	GCTCTTC	1 cut(s)	699
BspT104I	TTCGAA	1 cut(s)	120
BspT107I	GGYRCC	2 cut(s)	2114, 3806
BspTNI	GGTCTC	1 cut(s)	1563
BsrDI	GCAATG	5 cut(s)	204, 1040, 1835, 3543, 3997
BsrGI	TGTACA	1 cut(s)	96
BssECI	CCNNGG	8 cut(s)	621, 669, 1759, 1781, 2775, 3398, 3459, 4701
BssNAI	GTATAC	1 cut(s)	334
BssSI	CACGAG	1 cut(s)	4730
BssT1I	CCWWGG	5 cut(s)	621, 1781, 2775, 3459, 4701
Bst1107I	GTATAC	1 cut(s)	334
Bst2BI	CACGAG	1 cut(s)	4730
Bst2UI	CCWGG	5 cut(s)	431, 671, 1761, 3399, 3906
Bst6I	CTCTTC	3 cut(s)	56, 699, 4452
BstAUI	TGTACA	1 cut(s)	96
BstBAI	YACGTR	2 cut(s)	4024, 4238
BstBI	TTCGAA	1 cut(s)	120
BstC8I	GCNNGC	3 cut(s)	3082, 3811, 4637
BstDSI	CCRYGG	2 cut(s)	621, 4701
BstH2I	RGCGCY	1 cut(s)	4421
BstHHI	GCGC	2 cut(s)	953, 4420
BstMAI	GTCTC	8 cut(s)	616, 1503, 1563, 1736, 2156, 3357, 3837, 4200
BstMWI	GCNNNNNNNGC	9 cut(s)	889, 959, 1700, 2795, 2884, 3114, 3665, 3962, 4732
BstNI	CCWGG	5 cut(s)	431, 671, 1761, 3399, 3906
BstNSI	RCATGY	3 cut(s)	713, 3788, 4290
BstPAI	GACNNNNGTC	1 cut(s)	363
BstSCI	CCNGG	5 cut(s)	429, 669, 1759, 3397, 3904
BstSFI	CTRYAG	3 cut(s)	720, 2025, 4710
BstSLI	GKGCMC	2 cut(s)	1010, 3459
BstSNI	TACGTA	1 cut(s)	4238
BstV2I	GAAGAC	5 cut(s)	693, 864, 4086, 4211, 4700
BstX2I	RGATCY	7 cut(s)	1108, 1630, 2230, 2369, 3421, 3856, 4675
BstXI	CCANNNNNNTGG	3 cut(s)	375, 3387, 4075
BstYI	RGATCY	7 cut(s)	1108, 1630, 2230, 2369, 3421, 3856, 4675
BstZ17I	GTATAC	1 cut(s)	334
Bsu15I	ATCGAT	1 cut(s)	4467
BsuI	GTATCC	4 cut(s)	1139, 2868, 3347, 3436
BsuRI	GGCC	9 cut(s)	237, 263, 838, 1786, 2129, 3108, 3563, 3776, 4528
BsuTUI	ATCGAT	1 cut(s)	4467
BtgI	CCRYGG	2 cut(s)	621, 4701
BtrI	CACGTC	1 cut(s)	2212
BtsI	GCAGTG	2 cut(s)	156, 4529
BveI	ACCTGC	3 cut(s)	2018, 2916, 3327
Cac8I	GCNNGC	3 cut(s)	3082, 3811, 4637
CaiI	CAGNNNCTG	5 cut(s)	74, 2033, 3816, 3935, 4055
CciI	TCATGA	1 cut(s)	411
CfoI	GCGC	2 cut(s)	953, 4420
Cfr13I	GGNCC	5 cut(s)	262, 836, 3562, 3647, 4460
ClaI	ATCGAT	1 cut(s)	4467
CspCI	CAANNNNNGTGG	2 cut(s)	1312, 1347
DraI	TTTAAA	1 cut(s)	4183
DraIII	CACNNNGTG	1 cut(s)	1141
DrdI	GACNNNNNNGTC	1 cut(s)	1724
DseDI	GACNNNNNNGTC	1 cut(s)	1724
EaeI	YGGCCR	3 cut(s)	235, 3106, 4526
Eam1104I	CTCTTC	3 cut(s)	56, 699, 4452
EarI	CTCTTC	3 cut(s)	56, 699, 4452
EciI	GGCGGA	1 cut(s)	607
Ecl136II	GAGCTC	1 cut(s)	3237
Eco105I	TACGTA	1 cut(s)	4238
Eco130I	CCWWGG	5 cut(s)	621, 1781, 2775, 3459, 4701
Eco147I	AGGCCT	1 cut(s)	2129
Eco24I	GRGCYC	1 cut(s)	3239
Eco31I	GGTCTC	1 cut(s)	1563
Eco32I	GATATC	1 cut(s)	642
Eco47I	GGWCC	2 cut(s)	3647, 4460
Eco53kI	GAGCTC	1 cut(s)	3237
Eco57I	CTGAAG	3 cut(s)	2244, 2390, 2873
Eco88I	CYCGRG	3 cut(s)	1500, 3260, 4421
EcoICRI	GAGCTC	1 cut(s)	3237
EcoO109I	RGGNCCY	1 cut(s)	3562
EcoRI	GAATTC	3 cut(s)	1428, 4538, 4577
EcoRII	CCWGG	5 cut(s)	429, 669, 1759, 3397, 3904
EcoRV	GATATC	1 cut(s)	642
EcoT14I	CCWWGG	5 cut(s)	621, 1781, 2775, 3459, 4701
EcoT22I	ATGCAT	2 cut(s)	451, 1681
EcoT38I	GRGCYC	1 cut(s)	3239
ErhI	CCWWGG	5 cut(s)	621, 1781, 2775, 3459, 4701
FalI	AAGNNNNNCTT	6 cut(s)	1971, 2003, 2077, 2109, 4329, 4361
FaqI	GGGAC	4 cut(s)	1518, 1565, 2757, 3052
FauNDI	CATATG	2 cut(s)	955, 2976
FbaI	TGATCA	4 cut(s)	211, 1068, 1957, 2917
FblI	GTMKAC	2 cut(s)	311, 333
FriOI	GRGCYC	1 cut(s)	3239
FspAI	RTGCGCAY	1 cut(s)	952
FspI	TGCGCA	1 cut(s)	952
GlaI	GCGC	2 cut(s)	952, 4419
GsaI	CCCAGC	1 cut(s)	171
GsuI	CTGGAG	9 cut(s)	382, 998, 1698, 1719, 1782, 3083, 3209, 3381, 3927
HaeII	RGCGCY	1 cut(s)	4421
HaeIII	GGCC	9 cut(s)	237, 263, 838, 1786, 2129, 3108, 3563, 3776, 4528
HapII	CCGG	4 cut(s)	1314, 1459, 3645, 4369
HhaI	GCGC	2 cut(s)	953, 4420
Hin6I	GCGC	2 cut(s)	951, 4418
HinP1I	GCGC	2 cut(s)	951, 4418
HincII	GTYRAC	2 cut(s)	3016, 3915
HindII	GTYRAC	2 cut(s)	3016, 3915
HindIII	AAGCTT	6 cut(s)	789, 1912, 1920, 2259, 3734, 4151
HpaII	CCGG	4 cut(s)	1314, 1459, 3645, 4369
HphI	GGTGA	5 cut(s)	582, 2925, 4118, 4493, 4595
Hpy166II	GTNNAC	8 cut(s)	97, 312, 334, 1008, 3016, 3915, 4241, 4484
Hpy8I	GTNNAC	8 cut(s)	97, 312, 334, 1008, 3016, 3915, 4241, 4484
Hpy99I	CGWCG	3 cut(s)	3294, 3387, 4746
HpyAV	CCTTC	7 cut(s)	1351, 1381, 1805, 3266, 3989, 4221, 4408
HpyCH4IV	ACGT	7 cut(s)	2211, 2265, 2837, 3018, 3385, 4023, 4237
HpyF10VI	GCNNNNNNNGC	9 cut(s)	889, 959, 1700, 2795, 2884, 3114, 3665, 3962, 4732
HpySE526I	ACGT	7 cut(s)	2211, 2265, 2837, 3018, 3385, 4023, 4237
HspAI	GCGC	2 cut(s)	951, 4418
Ksp22I	TGATCA	4 cut(s)	211, 1068, 1957, 2917
LguI	GCTCTTC	1 cut(s)	699
LmnI	GCTCC	9 cut(s)	341, 1451, 1700, 1761, 3190, 3242, 3400, 3572, 4130
MaeII	ACGT	7 cut(s)	2211, 2265, 2837, 3018, 3385, 4023, 4237
MfeI	CAATTG	4 cut(s)	2673, 4200, 4223, 4572
MflI	RGATCY	7 cut(s)	1108, 1630, 2230, 2369, 3421, 3856, 4675
MhlI	GDGCHC	6 cut(s)	1010, 1513, 2435, 3239, 3459, 4062
MlsI	TGGCCA	3 cut(s)	237, 3108, 4528
MluNI	TGGCCA	3 cut(s)	237, 3108, 4528
MlyI	GAGTC	8 cut(s)	373, 1124, 1379, 1712, 2779, 3074, 3839, 4027
MmeI	TCCRAC	4 cut(s)	135, 3478, 3834, 4333
Mox20I	TGGCCA	3 cut(s)	237, 3108, 4528
Mph1103I	ATGCAT	2 cut(s)	451, 1681
MroXI	GAANNNNTTC	5 cut(s)	116, 1080, 1173, 2518, 4341
MscI	TGGCCA	3 cut(s)	237, 3108, 4528
MslI	CAYNNNNRTG	5 cut(s)	2292, 2428, 3166, 3327, 3430
Msp20I	TGGCCA	3 cut(s)	237, 3108, 4528
MspA1I	CMGCKG	2 cut(s)	3932, 4333
MspI	CCGG	4 cut(s)	1314, 1459, 3645, 4369
MspR9I	CCNGG	5 cut(s)	431, 671, 1761, 3399, 3906
MunI	CAATTG	4 cut(s)	2673, 4200, 4223, 4572
Mva1269I	GAATGC	4 cut(s)	451, 497, 988, 2073
MvaI	CCWGG	5 cut(s)	431, 671, 1761, 3399, 3906
MwoI	GCNNNNNNNGC	9 cut(s)	889, 959, 1700, 2795, 2884, 3114, 3665, 3962, 4732
NcoI	CCATGG	2 cut(s)	621, 4701
NdeI	CATATG	2 cut(s)	955, 2976
NlaIV	GGNNCC	5 cut(s)	264, 2116, 2371, 3649, 3808
NmuCI	GTSAC	5 cut(s)	1240, 3518, 4019, 4106, 4204
NsbI	TGCGCA	1 cut(s)	952
NsiI	ATGCAT	2 cut(s)	451, 1681
NspI	RCATGY	3 cut(s)	713, 3788, 4290
NspV	TTCGAA	1 cut(s)	120
OliI	CACNNNNGTG	1 cut(s)	2428
PaeR7I	CTCGAG	1 cut(s)	4421
PagI	TCATGA	1 cut(s)	411
PaqCI	CACCTGC	1 cut(s)	2018
PceI	AGGCCT	1 cut(s)	2129
PciI	ACATGT	1 cut(s)	4286
PciSI	GCTCTTC	1 cut(s)	699
PctI	GAATGC	4 cut(s)	451, 497, 988, 2073
PdmI	GAANNNNTTC	5 cut(s)	116, 1080, 1173, 2518, 4341
PfoI	TCCNGGA	2 cut(s)	429, 3904
PleI	GAGTC	8 cut(s)	372, 1123, 1378, 1712, 2778, 3073, 3839, 4026
PpsI	GAGTC	8 cut(s)	372, 1123, 1378, 1712, 2778, 3073, 3839, 4026
Ppu21I	YACGTR	2 cut(s)	4024, 4238
PscI	ACATGT	1 cut(s)	4286
PshAI	GACNNNNGTC	1 cut(s)	363
PshBI	ATTAAT	1 cut(s)	864
PsiI	TTATAA	1 cut(s)	302
Psp124BI	GAGCTC	1 cut(s)	3239
Psp6I	CCWGG	5 cut(s)	429, 669, 1759, 3397, 3904
PspFI	CCCAGC	1 cut(s)	167
PspGI	CCWGG	5 cut(s)	429, 669, 1759, 3397, 3904
PspN4I	GGNNCC	5 cut(s)	264, 2116, 2371, 3649, 3808
PspPI	GGNCC	5 cut(s)	262, 836, 3562, 3647, 4460
PspXI	VCTCGAGB	1 cut(s)	4421
PstI	CTGCAG	3 cut(s)	724, 2029, 4714
PstNI	CAGNNNCTG	5 cut(s)	74, 2033, 3816, 3935, 4055
PsuI	RGATCY	7 cut(s)	1108, 1630, 2230, 2369, 3421, 3856, 4675
PvuII	CAGCTG	2 cut(s)	3932, 4333
RseI	CAYNNNNRTG	5 cut(s)	2292, 2428, 3166, 3327, 3430
SacI	GAGCTC	1 cut(s)	3239
SapI	GCTCTTC	1 cut(s)	699
Sau96I	GGNCC	5 cut(s)	262, 836, 3562, 3647, 4460
ScaI	AGTACT	2 cut(s)	1601, 2825
SchI	GAGTC	8 cut(s)	373, 1124, 1379, 1712, 2779, 3074, 3839, 4027
ScrFI	CCNGG	5 cut(s)	431, 671, 1761, 3399, 3906
SduI	GDGCHC	6 cut(s)	1010, 1513, 2435, 3239, 3459, 4062
SfcI	CTRYAG	3 cut(s)	720, 2025, 4710
Sfr274I	CTCGAG	1 cut(s)	4421
SfuI	TTCGAA	1 cut(s)	120
SinI	GGWCC	2 cut(s)	3647, 4460
SlaI	CTCGAG	1 cut(s)	4421
SmiMI	CAYNNNNRTG	5 cut(s)	2292, 2428, 3166, 3327, 3430
SmlI	CTYRAG	4 cut(s)	1112, 2570, 3957, 4421
SmoI	CTYRAG	4 cut(s)	1112, 2570, 3957, 4421
SnaBI	TACGTA	1 cut(s)	4238
SpeI	ACTAGT	3 cut(s)	1864, 2185, 4306
SseBI	AGGCCT	1 cut(s)	2129
SsiI	CCGC	9 cut(s)	442, 618, 892, 1850, 2586, 2878, 3435, 3925, 4232
SspI	AATATT	2 cut(s)	374, 4191
SstI	GAGCTC	1 cut(s)	3239
StuI	AGGCCT	1 cut(s)	2129
StyD4I	CCNGG	5 cut(s)	429, 669, 1759, 3397, 3904
StyI	CCWWGG	5 cut(s)	621, 1781, 2775, 3459, 4701
TaiI	ACGT	7 cut(s)	2214, 2268, 2840, 3021, 3388, 4026, 4240
TatI	WGTACW	6 cut(s)	96, 1599, 2179, 2823, 2848, 4289
TauI	GCSGC	1 cut(s)	1853
TseFI	GTSAC	5 cut(s)	1240, 3518, 4019, 4106, 4204
Tsp45I	GTSAC	5 cut(s)	1240, 3518, 4019, 4106, 4204
TspGWI	ACGGA	2 cut(s)	354, 4759
VneI	GTGCAC	1 cut(s)	1006
VpaK11BI	GGWCC	2 cut(s)	3647, 4460
VspI	ATTAAT	1 cut(s)	864
XbaI	TCTAGA	2 cut(s)	3145, 3410
XceI	RCATGY	3 cut(s)	713, 3788, 4290
XcmI	CCANNNNNNNNNTGG	1 cut(s)	503
XhoI	CTCGAG	1 cut(s)	4421
XmaJI	CCTAGG	1 cut(s)	1781
XmiI	GTMKAC	2 cut(s)	311, 333
XmnI	GAANNNNTTC	5 cut(s)	116, 1080, 1173, 2518, 4341
ZrmI	AGTACT	2 cut(s)	1601, 2825
Zsp2I	ATGCAT	2 cut(s)	451, 1681

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.