Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0001909

Physical Location & Seq

Genomic Coordinates Reverse (-)

21643709 .. 21651537

Gene Structure 7829 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000012724

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4827 bp

ATGAGTCTCACTGCTCTTGACATTTCCCTTGAAGGATCACAATCTCCCCATGATATCAAGCATTTCGCCCCCAGAAGGTATCAAGTGAAAGTGTTCGAAGTCGCAAAGCGTAAGAACATAATTGCAGTGTTGGAAACTGGTGCTGGGAAAACTATGATTGCTGTGATGCTCATCAAACACATTGCTGATCAACACATAATCAAGTCCATTTGCCACAAGAAGTTGATAATTTTCATGGCCCCAACAGTTCATCTTGTCATTCAGCAATATGAGGTTATAAAAAAGTCTACAACTCTTGAAGTGGGAGTATACTACGGAGCTAAGGGCGTTGATGACTGGAGTCCAGAATATTGGGAGGTTGAAGTGAATACACATAATATAATGGTCATGACACCTCAAATTCTCCTGGATGCTTTGCGGAATGGATTTTTGAAGATAGAGATGATCTGCTTGATGGTCTTTGATGAATGCCACCGTGCCACTGGCAATCATCCCTACGCGAAAATAATGAAGGAATTTCATCACAAATCAGGAAACAAGCCAAAGATTTTTGGAATGACAGCATCACCAGTCATTAAAAAAGGTGTCTCATCCGCCACGGAATGTGAGGATCAGATATCACAACTTGAAAGCATCTTGGATTCCCAGGTTTATACAGTAGAAGACAGGACAGAGATTGAAGAGCATGTTCCCTCAGCAGTCCAAATTTGTAAATTTTATGACCCGACATGGTTGATCTGTGTGGATTTGAAAGCAAATATGGAAGCTTTATGGTCAAAGTTTGATGCTTCTATCATGTCAAAGTTGCAAGGGCCAGAGAAAAGCGAGTACGAAGACATTAATGATAAAATCAAGATGCTACGAAAGCGGTTGTCCAATGACTACATGAAAATTTTGTATTGCCTTGATGATCTTGGTATCGTATGCGCATATGAGGCTGTGAAAGTCTGTCTGGAGAATGCCCCTGATGTCAAAGAGGAGTGCACATTTTATAGAGAAAGCTCTTTGCAATGCAGATATTTTCTTGACGAAGTCTTAAATATGATCAGTGAATTTCTTCAAAAAGGTTATATACTTCATACAGATTTTGAGTCAGACTATATAAAGGCACACAAAGTGGGATACATATCTCCGAAGTTACATGAACTTCTTCAAATTTTCCACTCATTTGAGAGGGATAGGCAAGTTCTGTGCCTTATTTTCGTAGAGAGAATTGTCACAGCCAAAGTAATGCAAAGATATGTCAAGAAACTGCCATGTTTATCTCATTTCACAGCTTCATATTTGACCGGAAGTACCACATCAGTTGATGCACTTGCACCGAAATTGCAGAAGGAAATTTTGGAGTCATTTTACAGTGGGAAGGTCAATCTACTATTTGCCACTGATGTAGTTGAGGAGGGAATTCATGTCCAGAACTGCTCCTGTGTGATCCGGTTTGATCTTCCCAAGACAGTTCGTAGCTATGTCCAGTCTCGGGGACGAGCACGAAAAAATGATTCCCAATATATCATAATGCTTGAAAGGGACAACAAGAAACAAAGAGACCATCTCTTTGACATAATCAGGAGTGAGTACTCAATGACAGATACGTCGAGAAATCGAGATCCAGATGGATGCAATTTAAGAGCTTGTAACTTTGAGGATGCAAATGCATACTATGTAGAAGCTACTGGAGCATCAGTTACTCCAGACTCTAGTGTCAGCCTCATGTATCGGTACTGTGAAAAGCTGCCTGGAGATAGGTACTTTATTCCAAGGCCAAATTTTCACTTCAGACTATTTGAAGGGTTGTATCAGTGTGAAATAACATTACCTCCAAATGCGGCTTTTCAAACACTAGTTGGTCCTATGAGTAGAAACGGTCATTCATCAAAGCAGCTTGTATGCTTGGAAGCTTGTAAAATGCTGCATCAAATGGGTGCTTTGGATGATCATCTTCTCCCACATGTTGAAATGCCTCTTGATAATGAAGTGATTGTGAAAAGCAAAGAATTATCTGCAGGTGCTGGAACCACAAAAAGGAAAGAGTTACATGGGACGACTTGCATTCATGCATTAAGTGGAACTTGGGGAGAGAAAGTTGATGGTGCCAGTTTTGAGGCCTACAAATTTGAGTTCTCCTGTAATGTTGATGAGACATATTCAGGATTTGTACTACTAGTCGAGTTGAAACTTGATGATGACGTGGGAAATACTGAAGTGGATCTCTACTTAATTGCAAAGACAGTCAAAGCTTACGTTTCTTCATGTGGAAAGGTGCATCTCAGTGCTGATCAGATAAAGGAAGCCATGTGCTTTCAGGAATTTTTCTTTAATGGTTTGTTTGGGAGATTATTTCTTGGATCCGAAGCAAAAGGGACAGAAAGAAAATTTCTACTTCAGACTGAAATAAAATCACTATGGAGCTCATTGTACAGCTATTTACTTTTACCGACTGAGGCATCTGACAGTTCAAATAAGAAATCTTGGAACATAGATTGGATGGGAATCTGTTCCTGTGCTAATGTGGTGGAGTTTCTTAAGAAACACTATTCCAATGGTCTTGACCATTTCAAAGGCGGTAGAGGAAATATATTACTTTCTAGGACTGGTTCATCCATGACAGATTGCAAGGCTGAAAATTTAGTCCAGTTTGCTAATAGTTCAATTGACGTCAATGATCTTGAAGATGTGGTAGTATTGGCTATTCACACAGGAAGAATCTACTCTGTTATTGAAGTTGTTAGTAGCATTTCTGCTGAGAGTCCCTTGGAAGAAAATGCTGATGCTGCCTCATCAGATTATACTTATGTCGAGTACTTCAAAGCAACGTATGGGATTGTACTCAAGTATCCTGGACATCCTCTATTGCGGTTGAAGCAAAGTCATAATGCACATAACCTGCTTGTTGATCAAGGTGCGTCATCACCAGATGGTCTAGTTCAAGAAAAGCAACAAGCTCATGTTCATATGCCACCTGAAATCTTAGTCAGTTTTGATGTTCGAGTTGACGTTCTAAAATCATTTTACTTACTACCATCACTAATGTATCGTCTGGAGTCCCTAATGTTAGCCAGCCAAATTAGGAAAGAGATCAATGGCCAGTCTAGCAACTTTTATATATCAAGCTCATTGATTCTAGAAGCACTCACAACACTCGGATGCTGTGAAAAATTTTCATTGGAGCGTTTGGAGTTGCTTGGAGATTCAGTTCTCAAATATGCTGTGAGTTCCTACCTCTTTCTTAAATATCCAGAGAAACACGAAGGACAATTAACTGCTCGACGAACAAGAGCTATTTGCAACTCAAACCTGCATAAACTAGGAACGAAAAACAAGTTACAGGGATACATACGAGACAGTGCATTTGAACCACGACGTTGGGCTGCTCCAGGGCAGCTTTCTAGATTCCCGGATCCATGTCAATGCGGAGTGGATACTTTGGAAGTGCCCTTGGACAGTAGATTTCAAACTGAAAAGGCTGTTAAGGTTGGAAAATTCTGTGATAAAGGTCACAGATGGATGAACTCAAAAACCATTGCTGATTGTGTTGAGGCCCTCATAGGAGCTTATTATGTTGGTGGAGGATTGTTTGCTGCACTTCATTTGATGAAGTGGCTCAGCATTGATTCTGAAACCGGACCCTCATTAGCTGATGAAGCCATTACAAGGGCATCACTTCGATACTACAATCCCAAAACTAATGAACTTGCTAACTTGGAGTCGAAGCTTAACTATGAATTTTCAGTCAAGGGCTTGTTAGATGAGGCCATCACACATGCTTCTCAGCAAAAACTAGGTGCCAGCTACTGTTATCAGAGGCTTGAATTTCTTGGAGACTCTGTGTTGGATCTGCTAACTACACAGCATCTCTATCGCAGCCACACAGATATTGATCCTGGAGTGTTGACTGATTTGCGGTCAGCTGCTGTTAGTAATGAAAACTTTGCTCAAGCTGCTATTAGACAAAACCTTCATCCACATCTTCAACATTGCTCTGCGTTACTTCGGAGTCACATAATCGAGTATGAGAAGTTGCTTACAGAAGCTGAGAACAGTACCAGCTCACTGGAAGACATTAAGAGTCCAAAGGCTCTTGGTGACATGGTGGAAAGTATAGCAGGAGCTATACTAATTGATACAAAGCTTAATCTTGATGAAGTATGGAGGGTTTTTAAACCAATATTGTCTCCAATTGTGACCCCTTCTAGTCTTCAATTGCATCCGCTACGTAAACTGTCTGAATTATGTGATTCACTGGGGTACTTTGTGAAAGAAACATGTACTAAAGATGATACACTAGTGCATATGGAACTAAGTTTGCAGCTGGAAGATGTTCTTTTGGTTGGAAACGGCTTTGACCGGAGTAGAAAAGCTGCGAAACATAAGGCAGCTCGTCAAGTGTTGAAGGCGCTCGAGCAGAATATGACAACAATTCAATCATCTTCAGAGTCAAAAACAATAGATCCTACCCAATCCAATGACTGTTCTAATGAAGTCTTAACTCACAACATTGCAACCCCAGTTATTGGTCCAATCGATACGACAAAAGGTGAACCTCGAAAATCTCTCTTTAAGCTATGTAAGAAATTGCAGTGGCCAATGCCTGCATTCACAACAACAGAGCAGAAGTCAAGAACTCCAATTGAATTCGGTGAGGGTTCTGAAAAGAGAATAGGTTTCAGCAGCTTTCAATCAAAAATCAGCTTGCACATACCCAACTCTTGCACTATTGTATGCACAGGAGATCCTAGAGCCGATAAGAAGACTTCCATGGATTCTGCAGTTGTTTCCATGCTTCACGAGCTTCAACGACGGAGAAAACTTGTCATTCTCCAATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1609

Amino Acids

181.3

Weight (kDa)

6.39

Isoelectric Point (pI)

43.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	24 - 190	4.3e-16	Type III restriction enzyme, res subunit
DEAD	PF00270	26 - 190	5.1e-18	DEAD/DEAH box helicase
Helicase_C	PF00271	379 - 496	2.7e-17	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	567 - 651	1.4e-22	Dicer dimerisation domain
PAZ	PF02170	886 - 1009	8.6e-09	PAZ domain
Ribonuclease_3	PF00636	1056 - 1189	2.3e-21	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1056 - 1113	2.7e-07	Ribonuclease-III-like
Ribonucleas_3_3	PF14622	1246 - 1374	9.8e-14	Ribonuclease-III-like
Ribonuclease_3	PF00636	1267 - 1375	8.9e-21	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001909)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920
fragaria_vesca	FvH4_2g07790 FvH4_2g18930 FvH4_2g18930 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780
malus_domestica	MD08G1046300.v1.1 MD10G1163700.v1.1 MD15G1047500.v1.1
prunus_persica	Prupe.1G401900_v2.0.a1 Prupe.1G401900_v2.0.a1 Prupe.8G202000_v2.0.a1 Prupe.8G202000_v2.0.a1
pyrus_communis	pycom08g03830 pycom08g03840 pycom15g04670
rosa_chinensis	RchiOBHm_Chr6g0284721 RchiOBHm_Chr6g0312121
rosa_laevigata	RLG00000010353 RLG00000012724
rosa_multiflora	Rmu_sc0004065.1_g000007 Rmu_sc0007474.1_g000021
rosa_roxburghii	Rroxscaffold_7G00157050 Rroxscaffold_7G00184440
rosa_rugosa	Rorug06G0165900 Rorug06G0392600 Rorug06G0392700 Rorug06G0392800
rosa_samantha	Rh4CG118000 Rh6AG279000 Rh6AG509700 Rh6BG279000 Rh6BG520600 Rh6CG281300 Rh6CG526600 Rh6DG274000 Rh6DG512000
rosa_wichuraiana	Rw6G023980 Rw6G044350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	278
AarI	CACCTGC	1 cut(s)	1994
AasI	GACNNNNNNGTC	2 cut(s)	1591, 1700
AatII	GACGTC	1 cut(s)	2658
Acc16I	TGCGCA	1 cut(s)	928
Acc36I	ACCTGC	3 cut(s)	1994, 2892, 3303
AccB1I	GGYRCC	2 cut(s)	2090, 3782
AccB7I	CCANNNNNTGG	1 cut(s)	4511
AccI	GTMKAC	2 cut(s)	287, 309
AccII	CGCG	1 cut(s)	500
AciI	CCGC	9 cut(s)	418, 594, 868, 1826, 2562, 2854, 3411, 3901, 4208
AcoI	YGGCCR	2 cut(s)	3082, 4580
AcuI	CTGAAG	4 cut(s)	1759, 2220, 2366, 4413
AcyI	GRCGYC	1 cut(s)	2655
AdeI	CACNNNGTG	1 cut(s)	1117
AfiI	CCNNNNNNNGG	5 cut(s)	75, 1477, 2022, 3651, 4511
AflII	CTTAAG	1 cut(s)	2522
AflIII	ACRYGT	2 cut(s)	1948, 4262
AhlI	ACTAGT	3 cut(s)	1840, 2161, 4282
AjiI	CACGTC	1 cut(s)	2188
AjnI	CCWGG	6 cut(s)	405, 645, 1735, 2836, 3373, 3880
AjuI	GAANNNNNNNTTGG	2 cut(s)	1325, 1357
AloI	GAACNNNNNNTCC	2 cut(s)	3177, 3209
Alw21I	GWGCWC	3 cut(s)	986, 1489, 2411
Alw26I	GTCTC	8 cut(s)	11, 592, 1479, 1539, 2132, 3333, 3813, 4176
Alw44I	GTGCAC	1 cut(s)	982
AlwNI	CAGNNNCTG	4 cut(s)	2009, 3792, 3911, 4031
Ama87I	CYCGRG	2 cut(s)	1476, 4397
AoxI	GGCC	8 cut(s)	237, 812, 1760, 2103, 3082, 3537, 3750, 4580
ApaLI	GTGCAC	1 cut(s)	982
ArsI	GACNNNNNNTTYG	4 cut(s)	1538, 1570, 3332, 3364
AseI	ATTAAT	1 cut(s)	840
Asp700I	GAANNNNTTC	5 cut(s)	92, 1056, 1149, 2494, 4317
AspLEI	GCGC	2 cut(s)	929, 4396
AspS9I	GGNCC	6 cut(s)	238, 812, 1847, 3538, 3623, 4514
AsuC2I	CCSGG	1 cut(s)	3395
AsuHPI	GGTGA	5 cut(s)	558, 2901, 4094, 4547, 4649
AsuII	TTCGAA	1 cut(s)	96
AvaI	CYCGRG	2 cut(s)	1476, 4397
AvaII	GGWCC	3 cut(s)	1847, 3623, 4514
BaeGI	GKGCMC	2 cut(s)	986, 3435
BaeI	ACNNNNGTAYC	6 cut(s)	2816, 2849, 3014, 3047, 4269, 4302
BalI	TGGCCA	2 cut(s)	3084, 4582
BamHI	GGATCC	2 cut(s)	2345, 3397
BanI	GGYRCC	2 cut(s)	2090, 3782
BanII	GRGCYC	1 cut(s)	2411
BauI	CACGAG	1 cut(s)	4784
BbsI	GAAGAC	5 cut(s)	669, 840, 4062, 4187, 4754
Bbv12I	GWGCWC	3 cut(s)	986, 1489, 2411
BbvCI	CCTCAGC	1 cut(s)	694
BccI	CCATC	9 cut(s)	448, 1557, 1607, 2081, 2479, 2909, 3028, 3495, 3761
BceAI	ACGGC	1 cut(s)	4351
BcgI	CGANNNNNNTGC	2 cut(s)	3839, 3873
BciT130I	CCWGG	6 cut(s)	407, 647, 1737, 2838, 3375, 3882
BciVI	GTATCC	4 cut(s)	1115, 2844, 3323, 3412
BclI	TGATCA	5 cut(s)	187, 1044, 1933, 2275, 2893
BcnI	CCSGG	1 cut(s)	3395
BcoDI	GTCTC	8 cut(s)	11, 592, 1479, 1539, 2132, 3333, 3813, 4176
BcuI	ACTAGT	3 cut(s)	1840, 2161, 4282
BfmI	CTRYAG	2 cut(s)	2001, 4764
BfoI	RGCGCY	1 cut(s)	4397
BfrI	CTTAAG	1 cut(s)	2522
BfuAI	ACCTGC	3 cut(s)	1994, 2892, 3303
BfuI	GTATCC	4 cut(s)	1115, 2844, 3323, 3412
BlpI	GCTNAGC	1 cut(s)	3602
BmcAI	AGTACT	2 cut(s)	1577, 2801
Bme1390I	CCNGG	7 cut(s)	407, 647, 1737, 2838, 3375, 3395, 3882
Bme18I	GGWCC	3 cut(s)	1847, 3623, 4514
BmeT110I	CYCGRG	2 cut(s)	1476, 4397
BmgBI	CACGTC	1 cut(s)	2188
BmgT120I	GGNCC	6 cut(s)	238, 812, 1847, 3538, 3623, 4514
BmiI	GGNNCC	7 cut(s)	240, 2014, 2092, 2347, 3399, 3625, 3784
BmrFI	CCNGG	7 cut(s)	407, 647, 1737, 2838, 3375, 3395, 3882
BmrI	ACTGGG	2 cut(s)	4250, 4499
BmuI	ACTGGG	2 cut(s)	4250, 4499
BoxI	GACNNNNGTC	1 cut(s)	339
BpiI	GAAGAC	5 cut(s)	669, 840, 4062, 4187, 4754
BplI	GAGNNNNNCTC	2 cut(s)	1536, 1568
BpmI	CTGGAG	8 cut(s)	358, 974, 1674, 1695, 1758, 3059, 3357, 3903
Bpu10I	CCTNAGC	2 cut(s)	321, 694
Bpu1102I	GCTNAGC	1 cut(s)	3602
Bpu14I	TTCGAA	1 cut(s)	96
BpuEI	CTTGAG	2 cut(s)	2813, 3918
BpuMI	CCSGG	1 cut(s)	3395
Bsa29I	ATCGAT	1 cut(s)	4521
BsaAI	YACGTR	1 cut(s)	4214
BsaBI	GATNNNNATC	6 cut(s)	40, 170, 1126, 1935, 2489, 3876
BsaHI	GRCGYC	1 cut(s)	2655
BsaI	GGTCTC	1 cut(s)	1539
BsaJI	CCNNGG	7 cut(s)	597, 645, 1757, 2751, 3374, 3435, 4755
BsaWI	WCCGGW	4 cut(s)	1289, 1434, 3620, 4344
Bsc4I	CCNNNNNNNGG	5 cut(s)	75, 1477, 2022, 3651, 4511
Bse3DI	GCAATG	5 cut(s)	180, 1016, 3519, 3973, 4494
Bse8I	GATNNNNATC	6 cut(s)	40, 170, 1126, 1935, 2489, 3876
BseBI	CCWGG	6 cut(s)	407, 647, 1737, 2838, 3375, 3882
BseCI	ATCGAT	1 cut(s)	4521
BseDI	CCNNGG	7 cut(s)	597, 645, 1757, 2751, 3374, 3435, 4755
BseJI	GATNNNNATC	6 cut(s)	40, 170, 1126, 1935, 2489, 3876
BseLI	CCNNNNNNNGG	5 cut(s)	75, 1477, 2022, 3651, 4511
BseMI	GCAATG	5 cut(s)	180, 1016, 3519, 3973, 4494
BseMII	CTCAG	7 cut(s)	708, 2281, 2430, 2733, 3616, 3782, 4023
BseRI	GAGGAG	2 cut(s)	992, 1412
BseSI	GKGCMC	2 cut(s)	986, 3435
BseYI	CCCAGC	1 cut(s)	143
BsgI	GTGCAG	1 cut(s)	3564
Bsh1236I	CGCG	1 cut(s)	500
BshFI	GGCC	8 cut(s)	239, 814, 1762, 2105, 3084, 3539, 3752, 4582
BshNI	GGYRCC	2 cut(s)	2090, 3782
BshVI	ATCGAT	1 cut(s)	4521
BsiHKAI	GWGCWC	3 cut(s)	986, 1489, 2411
BsiHKCI	CYCGRG	2 cut(s)	1476, 4397
BsiSI	CCGG	5 cut(s)	1290, 1435, 3395, 3621, 4345
BslFI	GGGAC	6 cut(s)	1494, 1541, 2053, 2374, 2733, 3028
BslI	CCNNNNNNNGG	5 cut(s)	75, 1477, 2022, 3651, 4511
BsmAI	GTCTC	8 cut(s)	11, 592, 1479, 1539, 2132, 3333, 3813, 4176
BsmFI	GGGAC	6 cut(s)	1494, 1541, 2053, 2374, 2733, 3028
BsmI	GAATGC	4 cut(s)	473, 964, 2049, 4592
BsnI	GGCC	8 cut(s)	239, 814, 1762, 2105, 3084, 3539, 3752, 4582
Bso31I	GGTCTC	1 cut(s)	1539
BsoBI	CYCGRG	2 cut(s)	1476, 4397
Bsp119I	TTCGAA	1 cut(s)	96
Bsp1286I	GDGCHC	4 cut(s)	986, 1489, 2411, 3435
Bsp1407I	TGTACA	1 cut(s)	2415
Bsp1720I	GCTNAGC	1 cut(s)	3602
Bsp19I	CCATGG	1 cut(s)	4755
BspACI	CCGC	9 cut(s)	418, 594, 868, 1826, 2562, 2854, 3411, 3901, 4208
BspANI	GGCC	8 cut(s)	239, 814, 1762, 2105, 3084, 3539, 3752, 4582
BspCNI	CTCAG	7 cut(s)	707, 2280, 2431, 2734, 3615, 3781, 4024
BspDI	ATCGAT	1 cut(s)	4521
BspFNI	CGCG	1 cut(s)	500
BspHI	TCATGA	1 cut(s)	387
BspLI	GGNNCC	7 cut(s)	240, 2014, 2092, 2347, 3399, 3625, 3784
BspMAI	CTGCAG	2 cut(s)	2005, 4768
BspMI	ACCTGC	3 cut(s)	1994, 2892, 3303
BspQI	GCTCTTC	1 cut(s)	675
BspT104I	TTCGAA	1 cut(s)	96
BspT107I	GGYRCC	2 cut(s)	2090, 3782
BspTI	CTTAAG	1 cut(s)	2522
BspTNI	GGTCTC	1 cut(s)	1539
BsrDI	GCAATG	5 cut(s)	180, 1016, 3519, 3973, 4494
BsrGI	TGTACA	1 cut(s)	2415
BssECI	CCNNGG	7 cut(s)	597, 645, 1757, 2751, 3374, 3435, 4755
BssNAI	GTATAC	1 cut(s)	310
BssNI	GRCGYC	1 cut(s)	2655
BssSI	CACGAG	1 cut(s)	4784
BssT1I	CCWWGG	4 cut(s)	1757, 2751, 3435, 4755
Bst1107I	GTATAC	1 cut(s)	310
Bst2BI	CACGAG	1 cut(s)	4784
Bst2UI	CCWGG	6 cut(s)	407, 647, 1737, 2838, 3375, 3882
Bst6I	CTCTTC	1 cut(s)	675
BstACI	GRCGYC	1 cut(s)	2655
BstAFI	CTTAAG	1 cut(s)	2522
BstAUI	TGTACA	1 cut(s)	2415
BstBAI	YACGTR	1 cut(s)	4214
BstBI	TTCGAA	1 cut(s)	96
BstC8I	GCNNGC	4 cut(s)	3058, 3787, 4590, 4691
BstDSI	CCRYGG	2 cut(s)	597, 4755
BstFNI	CGCG	1 cut(s)	500
BstH2I	RGCGCY	1 cut(s)	4397
BstHHI	GCGC	2 cut(s)	929, 4396
BstMAI	GTCTC	8 cut(s)	11, 592, 1479, 1539, 2132, 3333, 3813, 4176
BstMWI	GCNNNNNNNGC	9 cut(s)	865, 935, 1676, 2771, 2860, 3090, 3641, 3938, 4786
BstNI	CCWGG	6 cut(s)	407, 647, 1737, 2838, 3375, 3882
BstNSI	RCATGY	4 cut(s)	689, 1952, 3764, 4266
BstPAI	GACNNNNGTC	1 cut(s)	339
BstSCI	CCNGG	7 cut(s)	405, 645, 1735, 2836, 3373, 3393, 3880
BstSFI	CTRYAG	2 cut(s)	2001, 4764
BstSLI	GKGCMC	2 cut(s)	986, 3435
BstSNI	TACGTA	1 cut(s)	4214
BstUI	CGCG	1 cut(s)	500
BstV2I	GAAGAC	5 cut(s)	669, 840, 4062, 4187, 4754
BstX2I	RGATCY	7 cut(s)	1606, 2206, 2345, 3397, 3832, 4447, 4729
BstXI	CCANNNNNNTGG	3 cut(s)	351, 3363, 4051
BstYI	RGATCY	7 cut(s)	1606, 2206, 2345, 3397, 3832, 4447, 4729
BstZ17I	GTATAC	1 cut(s)	310
Bsu15I	ATCGAT	1 cut(s)	4521
BsuI	GTATCC	4 cut(s)	1115, 2844, 3323, 3412
BsuRI	GGCC	8 cut(s)	239, 814, 1762, 2105, 3084, 3539, 3752, 4582
BsuTUI	ATCGAT	1 cut(s)	4521
BtgI	CCRYGG	2 cut(s)	597, 4755
BtrI	CACGTC	1 cut(s)	2188
BtsI	GCAGTG	3 cut(s)	9, 132, 4583
BveI	ACCTGC	3 cut(s)	1994, 2892, 3303
Cac8I	GCNNGC	4 cut(s)	3058, 3787, 4590, 4691
CaiI	CAGNNNCTG	4 cut(s)	2009, 3792, 3911, 4031
CciI	TCATGA	1 cut(s)	387
CfoI	GCGC	2 cut(s)	929, 4396
Cfr13I	GGNCC	6 cut(s)	238, 812, 1847, 3538, 3623, 4514
ClaI	ATCGAT	1 cut(s)	4521
CseI	GACGC	1 cut(s)	2892
CspCI	CAANNNNNGTGG	2 cut(s)	1288, 1323
DraI	TTTAAA	1 cut(s)	4159
DraIII	CACNNNGTG	1 cut(s)	1117
DrdI	GACNNNNNNGTC	2 cut(s)	1591, 1700
DseDI	GACNNNNNNGTC	2 cut(s)	1591, 1700
EaeI	YGGCCR	2 cut(s)	3082, 4580
Eam1104I	CTCTTC	1 cut(s)	675
EarI	CTCTTC	1 cut(s)	675
EciI	GGCGGA	1 cut(s)	583
Ecl136II	GAGCTC	1 cut(s)	2409
Eco105I	TACGTA	1 cut(s)	4214
Eco130I	CCWWGG	4 cut(s)	1757, 2751, 3435, 4755
Eco147I	AGGCCT	1 cut(s)	2105
Eco24I	GRGCYC	1 cut(s)	2411
Eco31I	GGTCTC	1 cut(s)	1539
Eco32I	GATATC	2 cut(s)	55, 618
Eco47I	GGWCC	3 cut(s)	1847, 3623, 4514
Eco53kI	GAGCTC	1 cut(s)	2409
Eco57I	CTGAAG	4 cut(s)	1759, 2220, 2366, 4413
Eco88I	CYCGRG	2 cut(s)	1476, 4397
EcoICRI	GAGCTC	1 cut(s)	2409
EcoO109I	RGGNCCY	1 cut(s)	3538
EcoRI	GAATTC	2 cut(s)	1404, 4631
EcoRII	CCWGG	6 cut(s)	405, 645, 1735, 2836, 3373, 3880
EcoRV	GATATC	2 cut(s)	55, 618
EcoT14I	CCWWGG	4 cut(s)	1757, 2751, 3435, 4755
EcoT22I	ATGCAT	2 cut(s)	1657, 2059
EcoT38I	GRGCYC	1 cut(s)	2411
ErhI	CCWWGG	4 cut(s)	1757, 2751, 3435, 4755
FalI	AAGNNNNNCTT	4 cut(s)	2053, 2085, 4305, 4337
FaqI	GGGAC	6 cut(s)	1494, 1541, 2053, 2374, 2733, 3028
FauNDI	CATATG	3 cut(s)	931, 2952, 4290
FbaI	TGATCA	5 cut(s)	187, 1044, 1933, 2275, 2893
FblI	GTMKAC	2 cut(s)	287, 309
FriOI	GRGCYC	1 cut(s)	2411
FspAI	RTGCGCAY	1 cut(s)	928
FspI	TGCGCA	1 cut(s)	928
GlaI	GCGC	2 cut(s)	928, 4395
GsaI	CCCAGC	1 cut(s)	147
GsuI	CTGGAG	8 cut(s)	358, 974, 1674, 1695, 1758, 3059, 3357, 3903
HaeII	RGCGCY	1 cut(s)	4397
HaeIII	GGCC	8 cut(s)	239, 814, 1762, 2105, 3084, 3539, 3752, 4582
HapII	CCGG	5 cut(s)	1290, 1435, 3395, 3621, 4345
HgaI	GACGC	1 cut(s)	2892
HhaI	GCGC	2 cut(s)	929, 4396
Hin1I	GRCGYC	1 cut(s)	2655
Hin6I	GCGC	2 cut(s)	927, 4394
HinP1I	GCGC	2 cut(s)	927, 4394
HincII	GTYRAC	2 cut(s)	2992, 3891
HindII	GTYRAC	2 cut(s)	2992, 3891
HindIII	AAGCTT	5 cut(s)	765, 1896, 2235, 3710, 4127
HpaII	CCGG	5 cut(s)	1290, 1435, 3395, 3621, 4345
HphI	GGTGA	5 cut(s)	558, 2901, 4094, 4547, 4649
Hpy166II	GTNNAC	7 cut(s)	288, 310, 984, 2992, 3891, 4217, 4538
Hpy8I	GTNNAC	7 cut(s)	288, 310, 984, 2992, 3891, 4217, 4538
Hpy99I	CGWCG	4 cut(s)	1597, 3270, 3363, 4800
HpyCH4IV	ACGT	8 cut(s)	1592, 2187, 2241, 2655, 2813, 2994, 3361, 4213
HpyF10VI	GCNNNNNNNGC	9 cut(s)	865, 935, 1676, 2771, 2860, 3090, 3641, 3938, 4786
HpySE526I	ACGT	8 cut(s)	1592, 2187, 2241, 2655, 2813, 2994, 3361, 4213
Hsp92I	GRCGYC	1 cut(s)	2655
HspAI	GCGC	2 cut(s)	927, 4394
Ksp22I	TGATCA	5 cut(s)	187, 1044, 1933, 2275, 2893
LguI	GCTCTTC	1 cut(s)	675
LmnI	GCTCC	8 cut(s)	317, 1427, 1676, 2406, 3166, 3376, 3548, 4106
MaeII	ACGT	8 cut(s)	1592, 2187, 2241, 2655, 2813, 2994, 3361, 4213
MfeI	CAATTG	4 cut(s)	2649, 4176, 4199, 4626
MflI	RGATCY	7 cut(s)	1606, 2206, 2345, 3397, 3832, 4447, 4729
MhlI	GDGCHC	4 cut(s)	986, 1489, 2411, 3435
MlsI	TGGCCA	2 cut(s)	3084, 4582
MluNI	TGGCCA	2 cut(s)	3084, 4582
MmeI	TCCRAC	4 cut(s)	111, 3454, 3810, 4309
Mox20I	TGGCCA	2 cut(s)	3084, 4582
Mph1103I	ATGCAT	2 cut(s)	1657, 2059
MroXI	GAANNNNTTC	5 cut(s)	92, 1056, 1149, 2494, 4317
MscI	TGGCCA	2 cut(s)	3084, 4582
MslI	CAYNNNNRTG	3 cut(s)	2268, 3142, 3406
Msp20I	TGGCCA	2 cut(s)	3084, 4582
MspA1I	CMGCKG	2 cut(s)	3908, 4309
MspCI	CTTAAG	1 cut(s)	2522
MspI	CCGG	5 cut(s)	1290, 1435, 3395, 3621, 4345
MspR9I	CCNGG	7 cut(s)	407, 647, 1737, 2838, 3375, 3395, 3882
MunI	CAATTG	4 cut(s)	2649, 4176, 4199, 4626
Mva1269I	GAATGC	4 cut(s)	473, 964, 2049, 4592
MvaI	CCWGG	6 cut(s)	407, 647, 1737, 2838, 3375, 3882
MvnI	CGCG	1 cut(s)	500
MwoI	GCNNNNNNNGC	9 cut(s)	865, 935, 1676, 2771, 2860, 3090, 3641, 3938, 4786
NciI	CCSGG	1 cut(s)	3395
NcoI	CCATGG	1 cut(s)	4755
NdeI	CATATG	3 cut(s)	931, 2952, 4290
NlaIV	GGNNCC	7 cut(s)	240, 2014, 2092, 2347, 3399, 3625, 3784
NmuCI	GTSAC	5 cut(s)	1216, 3494, 3995, 4082, 4180
NsbI	TGCGCA	1 cut(s)	928
NsiI	ATGCAT	2 cut(s)	1657, 2059
NspI	RCATGY	4 cut(s)	689, 1952, 3764, 4266
NspV	TTCGAA	1 cut(s)	96
PaeR7I	CTCGAG	1 cut(s)	4397
PagI	TCATGA	1 cut(s)	387
PaqCI	CACCTGC	1 cut(s)	1994
PceI	AGGCCT	1 cut(s)	2105
PciI	ACATGT	2 cut(s)	1948, 4262
PciSI	GCTCTTC	1 cut(s)	675
PctI	GAATGC	4 cut(s)	473, 964, 2049, 4592
PdmI	GAANNNNTTC	5 cut(s)	92, 1056, 1149, 2494, 4317
PflFI	GACNNNGTC	1 cut(s)	1031
PflMI	CCANNNNNTGG	1 cut(s)	4511
PfoI	TCCNGGA	4 cut(s)	405, 2836, 3393, 3880
Ppu21I	YACGTR	1 cut(s)	4214
PscI	ACATGT	2 cut(s)	1948, 4262
PshAI	GACNNNNGTC	1 cut(s)	339
PshBI	ATTAAT	1 cut(s)	840
PsiI	TTATAA	1 cut(s)	278
Psp124BI	GAGCTC	1 cut(s)	2411
Psp6I	CCWGG	6 cut(s)	405, 645, 1735, 2836, 3373, 3880
PspFI	CCCAGC	1 cut(s)	143
PspGI	CCWGG	6 cut(s)	405, 645, 1735, 2836, 3373, 3880
PspN4I	GGNNCC	7 cut(s)	240, 2014, 2092, 2347, 3399, 3625, 3784
PspPI	GGNCC	6 cut(s)	238, 812, 1847, 3538, 3623, 4514
PspXI	VCTCGAGB	1 cut(s)	4397
PstI	CTGCAG	2 cut(s)	2005, 4768
PstNI	CAGNNNCTG	4 cut(s)	2009, 3792, 3911, 4031
PsuI	RGATCY	7 cut(s)	1606, 2206, 2345, 3397, 3832, 4447, 4729
PsyI	GACNNNGTC	1 cut(s)	1031
PvuII	CAGCTG	2 cut(s)	3908, 4309
RseI	CAYNNNNRTG	3 cut(s)	2268, 3142, 3406
SacI	GAGCTC	1 cut(s)	2411
SapI	GCTCTTC	1 cut(s)	675
Sau96I	GGNCC	6 cut(s)	238, 812, 1847, 3538, 3623, 4514
ScaI	AGTACT	2 cut(s)	1577, 2801
ScrFI	CCNGG	7 cut(s)	407, 647, 1737, 2838, 3375, 3395, 3882
SduI	GDGCHC	4 cut(s)	986, 1489, 2411, 3435
SfcI	CTRYAG	2 cut(s)	2001, 4764
Sfr274I	CTCGAG	1 cut(s)	4397
SfuI	TTCGAA	1 cut(s)	96
SinI	GGWCC	3 cut(s)	1847, 3623, 4514
SlaI	CTCGAG	1 cut(s)	4397
SmiMI	CAYNNNNRTG	3 cut(s)	2268, 3142, 3406
SmlI	CTYRAG	4 cut(s)	2522, 2828, 3933, 4397
SmoI	CTYRAG	4 cut(s)	2522, 2828, 3933, 4397
SnaBI	TACGTA	1 cut(s)	4214
SpeI	ACTAGT	3 cut(s)	1840, 2161, 4282
SseBI	AGGCCT	1 cut(s)	2105
SsiI	CCGC	9 cut(s)	418, 594, 868, 1826, 2562, 2854, 3411, 3901, 4208
SspI	AATATT	2 cut(s)	350, 4167
SstI	GAGCTC	1 cut(s)	2411
StuI	AGGCCT	1 cut(s)	2105
StyD4I	CCNGG	7 cut(s)	405, 645, 1735, 2836, 3373, 3393, 3880
StyI	CCWWGG	4 cut(s)	1757, 2751, 3435, 4755
TaiI	ACGT	8 cut(s)	1595, 2190, 2244, 2658, 2816, 2997, 3364, 4216
TatI	WGTACW	6 cut(s)	1575, 2155, 2415, 2799, 2824, 4265
TauI	GCSGC	1 cut(s)	1829
TseFI	GTSAC	5 cut(s)	1216, 3494, 3995, 4082, 4180
Tsp45I	GTSAC	5 cut(s)	1216, 3494, 3995, 4082, 4180
TspGWI	ACGGA	3 cut(s)	330, 614, 4813
Tth111I	GACNNNGTC	1 cut(s)	1031
Van91I	CCANNNNNTGG	1 cut(s)	4511
Vha464I	CTTAAG	1 cut(s)	2522
VneI	GTGCAC	1 cut(s)	982
VpaK11BI	GGWCC	3 cut(s)	1847, 3623, 4514
VspI	ATTAAT	1 cut(s)	840
XbaI	TCTAGA	2 cut(s)	3121, 3386
XceI	RCATGY	4 cut(s)	689, 1952, 3764, 4266
XcmI	CCANNNNNNNNNTGG	1 cut(s)	479
XhoI	CTCGAG	1 cut(s)	4397
XmiI	GTMKAC	2 cut(s)	287, 309
XmnI	GAANNNNTTC	5 cut(s)	92, 1056, 1149, 2494, 4317
ZraI	GACGTC	1 cut(s)	2656
ZrmI	AGTACT	2 cut(s)	1577, 2801
Zsp2I	ATGCAT	2 cut(s)	1657, 2059

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.