Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0001909

Physical Location & Seq

Genomic Coordinates Reverse (-)

23636340 .. 23645013

Gene Structure 8674 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00184440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3966 bp

ATGCCCCTGATGTCAAAGAGGAGTGCACATTTTATAGAAAGCTCTTTGCAATGCAGATATTTTCTTGATGAAGTCTTAAATATGATCAGTGAATTTCTTCAAAAAGGTTATATACTTCATACAGATCTTGAGTCAGACTATATAAAGGCACACAAAGTGGGATACATATCTCCGAAGTTACATGAACTTCTTCAAATTTTCCACTCATTTGAGAGGGATAGGCAAGTTCTGTGCCTTATTTTCGTAGAGAGAATTGTCACAGCCAAAGTAATGCAAAGATATGTGAAGAAACTGCCATGTTTATCTCATTTCACAGCTTCATATTTGACGGGAAGTACCACATCAGTTGATGCACTTGCACCGAAATTGCAGAAGGAAATTTTGGAGTCATTTTACAGTGGGAAGGTCAATCTACTATTTGCCACTGATGTAGTTGAAGAGGGAATTCATGTCCAGAACTGCTCCTGTGTGATCCGGTTTGATCTTCCCAAGACAGTTCGTAGCTATGTCCAGTCTCGGGGACGAGCAAGAAAAAATGATTCCCAATATATCATAATGCTTGAAAGGGACAACAAGAAGCAAAGAGACCATCTCTTTGACATAATCAGGAGTGAGTACTCAATGACAGATACGTCGAGAAATCGAGATCCTGATGGATGCAATTTAAGAGCTTGTAACTTTGAAGATGCAAATGCATACTATGTAGAAGCTACTGGTGCATCAGTTACTCCAGACTCTAGTGTCAGCCTCATATATCGGTACTGTGAAAAGCTCCCTGGAGATAGGTACTTTATTCCAAGGCCAACTTTTCATTTCAGACTATTTGAAGGGTTGTATCAGTGTGAAATAACATTACCTCCAAATGCGGCATTTCAAACACTAGTTGGTCCTATGAGTAGAAACGGTCATTCATCAAAGCAGCTTGTATGCTTGGAAGCTTGTAAAATGCTGCATCAAATGGGTGCTTTGGATGATCATCTTCTCCCACTTGTCGAAGTGCCTCTTGATAATGAAGTGATTGTGAAAAGCAAAGAATTATCTGCAGGTGCTGGAACTACAAAAAGGAAAGAGTTACATGGGATGACTTGCATTCGTGCATTAAGTGGAACTTGGGGAGAGAAAGTTGATGGTGCTAGTTTTGAGGCCTACAAATTTGAGTTCTCCTGTAATGTTGATGAGACATATTCAGGATTCGTACTACTAGTCGAGATGAAACTTGATGATGACGTGGGAAATACTGAAGTGGATCTCTACTTAATTGCAAAGATGGTCAAAGCTTACGTTTCTTCATGTGGAAAGGTGCATCTCAGTGCAGATCAGATAAAGGAAGCCATGTGCTTTCAGGAATTTTTCTTTAATGGTTTGTTTGGGAGATTATTTCTGGGATCCGTAGCAAAAGGGACAGAAAGAAAATTTCTACTTCAGACTGAAATAAAATCACTATGGTGCTCATCGTACAGCTATTTACTTTTACCGACTGAGGCATCTGACAGTTCAAATAAGAAATCTTGGAACATAGATTGGATGGGAATCTGTTCCTGTGCTAATGTGGTGGAGTTTCTTAGGAAACACTATTCCAATGGTCTTGAGAATTTCAAAGGCGGTAGAGGAAATATATTACTTTCTAGGACTGGTTCATCCATGACAGATTGCAAGGCTGAAAATTTAGTCCAGTTTGCTAATAGTTCAATTGATGTCAATGATCTTGAAGATATGGTAGTATTGGCTATTCACACAGGAAGAATCTACTCTGTTATTGAAGTTGTTAGTAGCATTTCTGCTGAGAGTCCCTTGGAAGAAAATGATGATACTGCCTCATCAGATTATACTTATGTTGAGTACTTCAAAACAACGTATGGGATTGTACTCAAGTATCCTAGACATCCTCTATTGCGGTTGAAGCAAAGTCATAATGCACATAACCTGCTTGTTGATCAAGGTGTGTCATCACCAGATGGTCTAGTTCAAGAAAAGCAACAAGCTCATGTTCATATGCCACCCGAAATCTTAGTCAGTATTGATGTTCGAGTTGACGTTCTAAAATCATTTTATTTACTACCATCACTAATGTATCGTCTGGAGTCCCTAATGTTAGCCAGCCAGATCAGGAAAGAGATCAATGGCCAGTCTAGCAACTTTTATATATCAAGCTCATTGATTCTAGAAGCACTCACAACACTTCGATGCTGTGAAAATTTTTCATTGGAGCGTTTGGAGTTGCTTGGAGATTCAGTTCTCAAATATGCTGTGAGCTCCTACCTCTTTCTTAAATATCCCGAGAAACATGAAGGACAATTAACTGCTCGACGAAAGAGAGCTATTTGCAACTCAAACCTGCATAACCTAGGAACGAAAAACAAGTTACAGGGATACATACGAGACAGTGCATTTGAACCACGACGTTGGGCTGCTCCAGGGCAGCTTTCTAGATTCCCAGATCCATGTCAATGCGGAGTGGATACTTTGGAAGTGCCCTTGGACAGTAGATTTCAAACTGAAAAGACTGTTAAGGTTGGAAAATTCTGTGATAAAGGTCACAGATGGATGAACTCAAAAACCATTGCTGATTGTGTTGAGGCCCTCATAGGAGCCTATTATGTTGGTGGAGGATTGTTTGCTGCACTTCATTTGATGAAGTGGCTCAGCATTGATTCTGAAACTGGATCCTCATTAGCTGATGAAGCCATTACAAGGGCATCACTTCGATCCTACAATCCCAAAACTAATGAACTTGCTAACTTGGAGTTGAAGCTTAGCTATGAATTTTCAGTCAAGGGCTTGTTACATGAGGCCATCACACATGCTTCTCAGCAAAAACTAGGTGCCAGCTACTGTTATCAGAGGCTTGAATTTCTTGGAGACTCTGTGTTGGATCTGCTAACTACACAGCATCTCTATCGCAGCCACACAGATATTGATCCTGGAGTGTTGACTGATTTGCGGTCAGCTGCTGTTAGTAATGAAAACTTTGCTCAAGTTGCTATTAGACAAAACCTTCATCCACATCTTCAACATTGCTCTGGGTTACTTCGGAGTCACATAACCGAGTATGAGAGGTTGCTTGCAGAAGCTGAGAACAGTACCAGCTCACTGGAAGACATTAAGGGTCCAAAGGCTCTTGGTGACATGGTGGAAAGTATAGCAGGAGCTATACTAATTGATACAAAGCTTAATGTTGATGAAGTATGGAGGGTTTTTAAACCAGTATTGTCTCCAATTGTGACCCCTTCTAGTCTTCAATTGCATCCGCTACGTAAACTGTCTGAATTATGTGATTCACTTGGGTACTTTGTGAAAGAAACATGTACTAAAGATGATACACTAGTGAATGTGGAACTAAGTTTGCAGCTGGAAGATGTTCTTTTGGTTGGAAACGGATTTGACCGGAGTAGAAAAGCTGCGAAACATAAGGCAGCTCGTCAAGTGTTGAAGGCGCTCGAGAAAAGCATAGAAAGGAGAGAGCATTATTCTGATATGGATAGTGACCACTGCAGCAACCTAACTGACGAAGATTTTTCAGAGCCAATGAGCCATGGAAAGCAGAATATGATTACAATTCAATCGTCTTCAGAGTCAAAAACAATAGATCCTACCCAATCCAATGACTGTTCTGATGAAGTCTTGAGTCACAACATTGCAACCCCAGTTATTGGTCCAATCGATACGAAAAAAGGTGAACCTCGAAAATCTCTCTTTGAGCTATGTAAGAAATTGCAGTGGCCAATGCCTACATTCACAACAACAGAGCAGAAGTCAAGAACTCCAATTGAATTCGGTGAGGGTTCTGAAAAGAGAATAGGTTTCAGCAGCTTTCAATCAAAAATCAGCTTGCACATACCCAACTCTTGCACTATTGTATGCACAGGAGATCCTGGAGCCGATAAGAAGACTTCCATGGATTCTGCAGTTGTTTCCATGCTTCACGAGCTTCATCGACGGAGAAAACTTGTCATTCTCCAGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1321

Amino Acids

148.71

Weight (kDa)

6.34

Isoelectric Point (pI)

43.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C	PF00271	59 - 176	2.1e-17	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	247 - 331	2.6e-23	Dicer dimerisation domain
PAZ	PF02170	566 - 685	1.4e-10	PAZ domain
Ribonuclease_3	PF00636	736 - 869	2.7e-21	Ribonuclease III domain
Ribonucleas_3_3	PF14622	736 - 793	4e-07	Ribonuclease-III-like
Ribonucleas_3_3	PF14622	926 - 1055	3.1e-14	Ribonuclease-III-like
Ribonuclease_3	PF00636	947 - 1055	1.4e-20	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001909)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920
fragaria_vesca	FvH4_2g07790 FvH4_2g18930 FvH4_2g18930 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780
malus_domestica	MD08G1046300.v1.1 MD10G1163700.v1.1 MD15G1047500.v1.1
prunus_persica	Prupe.1G401900_v2.0.a1 Prupe.1G401900_v2.0.a1 Prupe.8G202000_v2.0.a1 Prupe.8G202000_v2.0.a1
pyrus_communis	pycom08g03830 pycom08g03840 pycom15g04670
rosa_chinensis	RchiOBHm_Chr6g0284721 RchiOBHm_Chr6g0312121
rosa_laevigata	RLG00000010353 RLG00000012724
rosa_multiflora	Rmu_sc0004065.1_g000007 Rmu_sc0007474.1_g000021
rosa_roxburghii	Rroxscaffold_7G00157050 Rroxscaffold_7G00184440
rosa_rugosa	Rorug06G0165900 Rorug06G0392600 Rorug06G0392700 Rorug06G0392800
rosa_samantha	Rh4CG118000 Rh6AG279000 Rh6AG509700 Rh6BG279000 Rh6BG520600 Rh6CG281300 Rh6CG526600 Rh6DG274000 Rh6DG512000
rosa_wichuraiana	Rw6G023980 Rw6G044350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1034
AasI	GACNNNNNNGTC	2 cut(s)	631, 740
Acc36I	ACCTGC	3 cut(s)	1034, 1932, 2343
AccB1I	GGYRCC	1 cut(s)	2822
AccB7I	CCANNNNNTGG	1 cut(s)	3650
AciI	CCGC	6 cut(s)	866, 1602, 1894, 2451, 2941, 3248
AcoI	YGGCCR	2 cut(s)	2122, 3719
AcuI	CTGAAG	3 cut(s)	1260, 1406, 3552
AdeI	CACNNNGTG	1 cut(s)	157
AfiI	CCNNNNNNNGG	3 cut(s)	517, 2691, 3650
AflIII	ACRYGT	1 cut(s)	3302
AhlI	ACTAGT	3 cut(s)	880, 1201, 3322
AjiI	CACGTC	1 cut(s)	1228
AjnI	CCWGG	4 cut(s)	775, 2413, 2920, 3871
AjuI	GAANNNNNNNTTGG	2 cut(s)	365, 397
AleI	CACNNNNGTG	1 cut(s)	1444
AloI	GAACNNNNNNTCC	2 cut(s)	2217, 2249
Alw21I	GWGCWC	3 cut(s)	28, 1451, 2255
Alw26I	GTCTC	6 cut(s)	519, 579, 1172, 2373, 2853, 3216
Alw44I	GTGCAC	1 cut(s)	24
AlwNI	CAGNNNCTG	4 cut(s)	1049, 2832, 2951, 3071
Ama87I	CYCGRG	3 cut(s)	516, 2276, 3437
AoxI	GGCC	6 cut(s)	800, 1143, 2122, 2577, 2790, 3719
ApaLI	GTGCAC	1 cut(s)	24
ArsI	GACNNNNNNTTYG	6 cut(s)	578, 610, 1995, 2027, 2372, 2404
Asp700I	GAANNNNTTC	4 cut(s)	96, 189, 1534, 3357
AspA2I	CCTAGG	1 cut(s)	2344
AspLEI	GCGC	1 cut(s)	3436
AspS9I	GGNCC	4 cut(s)	887, 2578, 3107, 3653
AsuHPI	GGTGA	4 cut(s)	1941, 3134, 3686, 3788
AvaI	CYCGRG	3 cut(s)	516, 2276, 3437
AvaII	GGWCC	3 cut(s)	887, 3107, 3653
AvrII	CCTAGG	1 cut(s)	2344
BaeGI	GKGCMC	2 cut(s)	28, 2475
BaeI	ACNNNNGTAYC	6 cut(s)	1856, 1889, 2054, 2087, 3309, 3342
BalI	TGGCCA	2 cut(s)	2124, 3721
BamHI	GGATCC	2 cut(s)	1385, 2663
BanI	GGYRCC	1 cut(s)	2822
BanII	GRGCYC	1 cut(s)	2255
BauI	CACGAG	1 cut(s)	3923
BbsI	GAAGAC	4 cut(s)	3102, 3227, 3558, 3893
Bbv12I	GWGCWC	3 cut(s)	28, 1451, 2255
BccI	CCATC	9 cut(s)	597, 647, 1121, 1261, 1519, 1949, 2068, 2535, 2801
BcgI	CGANNNNNNTGC	2 cut(s)	2879, 2913
BciT130I	CCWGG	4 cut(s)	777, 2415, 2922, 3873
BciVI	GTATCC	4 cut(s)	155, 1884, 2363, 2452
BclI	TGATCA	3 cut(s)	84, 973, 1933
BcoDI	GTCTC	6 cut(s)	519, 579, 1172, 2373, 2853, 3216
BcuI	ACTAGT	3 cut(s)	880, 1201, 3322
BfmI	CTRYAG	3 cut(s)	1041, 3490, 3903
BfoI	RGCGCY	1 cut(s)	3437
BfuAI	ACCTGC	3 cut(s)	1034, 1932, 2343
BfuI	GTATCC	4 cut(s)	155, 1884, 2363, 2452
BglII	AGATCT	1 cut(s)	124
BlnI	CCTAGG	1 cut(s)	2344
BlpI	GCTNAGC	2 cut(s)	2642, 2753
BmcAI	AGTACT	2 cut(s)	617, 1841
Bme1390I	CCNGG	4 cut(s)	777, 2415, 2922, 3873
Bme18I	GGWCC	3 cut(s)	887, 3107, 3653
BmeT110I	CYCGRG	3 cut(s)	516, 2276, 3437
BmgBI	CACGTC	1 cut(s)	1228
BmgT120I	GGNCC	4 cut(s)	887, 2578, 3107, 3653
BmiI	GGNNCC	6 cut(s)	1387, 2590, 2665, 2824, 3108, 3877
BmrFI	CCNGG	4 cut(s)	777, 2415, 2922, 3873
BmrI	ACTGGG	1 cut(s)	3638
BmuI	ACTGGG	1 cut(s)	3638
BpiI	GAAGAC	4 cut(s)	3102, 3227, 3558, 3893
BplI	GAGNNNNNCTC	2 cut(s)	576, 608
BpmI	CTGGAG	7 cut(s)	714, 798, 2099, 2397, 2943, 3894, 3941
Bpu1102I	GCTNAGC	2 cut(s)	2642, 2753
BpuEI	CTTGAG	5 cut(s)	149, 1607, 1853, 2958, 3643
Bsa29I	ATCGAT	1 cut(s)	3660
BsaAI	YACGTR	1 cut(s)	3254
BsaBI	GATNNNNATC	4 cut(s)	166, 975, 1529, 2916
BsaI	GGTCTC	1 cut(s)	579
BsaJI	CCNNGG	8 cut(s)	775, 797, 1791, 2344, 2414, 2475, 3532, 3894
BsaWI	WCCGGW	2 cut(s)	474, 3384
BsaXI	ACNNNNNCTCC	8 cut(s)	841, 871, 1107, 1137, 2217, 2247, 2850, 2880
Bsc4I	CCNNNNNNNGG	3 cut(s)	517, 2691, 3650
Bse3DI	GCAATG	4 cut(s)	56, 2559, 3013, 3633
Bse8I	GATNNNNATC	4 cut(s)	166, 975, 1529, 2916
BseBI	CCWGG	4 cut(s)	777, 2415, 2922, 3873
BseCI	ATCGAT	1 cut(s)	3660
BseDI	CCNNGG	8 cut(s)	775, 797, 1791, 2344, 2414, 2475, 3532, 3894
BseGI	GGATG	9 cut(s)	662, 976, 1086, 1530, 1637, 1882, 2550, 2998, 3244
BseJI	GATNNNNATC	4 cut(s)	166, 975, 1529, 2916
BseLI	CCNNNNNNNGG	3 cut(s)	517, 2691, 3650
BseMI	GCAATG	4 cut(s)	56, 2559, 3013, 3633
BseMII	CTCAG	6 cut(s)	1321, 1470, 1773, 2656, 2822, 3063
BseRI	GAGGAG	1 cut(s)	34
BseSI	GKGCMC	2 cut(s)	28, 2475
BsgI	GTGCAG	2 cut(s)	1332, 2604
BshFI	GGCC	6 cut(s)	802, 1145, 2124, 2579, 2792, 3721
BshNI	GGYRCC	1 cut(s)	2822
BshVI	ATCGAT	1 cut(s)	3660
BsiHKAI	GWGCWC	3 cut(s)	28, 1451, 2255
BsiHKCI	CYCGRG	3 cut(s)	516, 2276, 3437
BsiSI	CCGG	2 cut(s)	475, 3385
BslFI	GGGAC	5 cut(s)	534, 581, 1414, 1773, 2068
BslI	CCNNNNNNNGG	3 cut(s)	517, 2691, 3650
BsmAI	GTCTC	6 cut(s)	519, 579, 1172, 2373, 2853, 3216
BsmFI	GGGAC	5 cut(s)	534, 581, 1414, 1773, 2068
BsmI	GAATGC	1 cut(s)	1089
BsnI	GGCC	6 cut(s)	802, 1145, 2124, 2579, 2792, 3721
Bso31I	GGTCTC	1 cut(s)	579
BsoBI	CYCGRG	3 cut(s)	516, 2276, 3437
Bsp1286I	GDGCHC	4 cut(s)	28, 1451, 2255, 2475
Bsp1720I	GCTNAGC	2 cut(s)	2642, 2753
Bsp19I	CCATGG	2 cut(s)	3532, 3894
BspACI	CCGC	6 cut(s)	866, 1602, 1894, 2451, 2941, 3248
BspANI	GGCC	6 cut(s)	802, 1145, 2124, 2579, 2792, 3721
BspCNI	CTCAG	6 cut(s)	1320, 1471, 1774, 2655, 2821, 3064
BspDI	ATCGAT	1 cut(s)	3660
BspLI	GGNNCC	6 cut(s)	1387, 2590, 2665, 2824, 3108, 3877
BspMAI	CTGCAG	3 cut(s)	1045, 3494, 3907
BspMI	ACCTGC	3 cut(s)	1034, 1932, 2343
BspT107I	GGYRCC	1 cut(s)	2822
BspTNI	GGTCTC	1 cut(s)	579
BsrDI	GCAATG	4 cut(s)	56, 2559, 3013, 3633
BssECI	CCNNGG	8 cut(s)	775, 797, 1791, 2344, 2414, 2475, 3532, 3894
BssSI	CACGAG	1 cut(s)	3923
BssT1I	CCWWGG	6 cut(s)	797, 1791, 2344, 2475, 3532, 3894
Bst2BI	CACGAG	1 cut(s)	3923
Bst2UI	CCWGG	4 cut(s)	777, 2415, 2922, 3873
Bst6I	CTCTTC	1 cut(s)	432
BstBAI	YACGTR	1 cut(s)	3254
BstC8I	GCNNGC	4 cut(s)	2098, 2827, 3063, 3830
BstDSI	CCRYGG	2 cut(s)	3532, 3894
BstF5I	GGATG	9 cut(s)	662, 976, 1086, 1530, 1637, 1882, 2550, 2998, 3244
BstH2I	RGCGCY	1 cut(s)	3437
BstHHI	GCGC	1 cut(s)	3436
BstMAI	GTCTC	6 cut(s)	519, 579, 1172, 2373, 2853, 3216
BstMWI	GCNNNNNNNGC	6 cut(s)	716, 1900, 2130, 2681, 2978, 3925
BstNI	CCWGG	4 cut(s)	777, 2415, 2922, 3873
BstNSI	RCATGY	2 cut(s)	2804, 3306
BstSCI	CCNGG	4 cut(s)	775, 2413, 2920, 3871
BstSFI	CTRYAG	3 cut(s)	1041, 3490, 3903
BstSLI	GKGCMC	2 cut(s)	28, 2475
BstSNI	TACGTA	1 cut(s)	3254
BstV2I	GAAGAC	4 cut(s)	3102, 3227, 3558, 3893
BstX2I	RGATCY	9 cut(s)	124, 646, 1246, 1385, 2437, 2663, 2872, 3586, 3868
BstXI	CCANNNNNNTGG	2 cut(s)	2403, 3091
BstYI	RGATCY	9 cut(s)	124, 646, 1246, 1385, 2437, 2663, 2872, 3586, 3868
Bsu15I	ATCGAT	1 cut(s)	3660
BsuI	GTATCC	4 cut(s)	155, 1884, 2363, 2452
BsuRI	GGCC	6 cut(s)	802, 1145, 2124, 2579, 2792, 3721
BsuTUI	ATCGAT	1 cut(s)	3660
BtgI	CCRYGG	2 cut(s)	3532, 3894
BtrI	CACGTC	1 cut(s)	1228
BtsCI	GGATG	9 cut(s)	662, 976, 1086, 1530, 1637, 1882, 2550, 2998, 3244
BtsI	GCAGTG	2 cut(s)	3487, 3722
BtsIMutI	CAGTG	9 cut(s)	94, 403, 423, 845, 1315, 2389, 3089, 3487, 3722
BveI	ACCTGC	3 cut(s)	1034, 1932, 2343
Cac8I	GCNNGC	4 cut(s)	2098, 2827, 3063, 3830
CaiI	CAGNNNCTG	4 cut(s)	1049, 2832, 2951, 3071
CfoI	GCGC	1 cut(s)	3436
Cfr13I	GGNCC	4 cut(s)	887, 2578, 3107, 3653
ClaI	ATCGAT	1 cut(s)	3660
CspCI	CAANNNNNGTGG	2 cut(s)	328, 363
DraI	TTTAAA	1 cut(s)	3199
DraIII	CACNNNGTG	1 cut(s)	157
DrdI	GACNNNNNNGTC	2 cut(s)	631, 740
DseDI	GACNNNNNNGTC	2 cut(s)	631, 740
EaeI	YGGCCR	2 cut(s)	2122, 3719
Eam1104I	CTCTTC	1 cut(s)	432
EarI	CTCTTC	1 cut(s)	432
Ecl136II	GAGCTC	1 cut(s)	2253
Eco105I	TACGTA	1 cut(s)	3254
Eco130I	CCWWGG	6 cut(s)	797, 1791, 2344, 2475, 3532, 3894
Eco147I	AGGCCT	1 cut(s)	1145
Eco24I	GRGCYC	1 cut(s)	2255
Eco31I	GGTCTC	1 cut(s)	579
Eco47I	GGWCC	3 cut(s)	887, 3107, 3653
Eco53kI	GAGCTC	1 cut(s)	2253
Eco57I	CTGAAG	3 cut(s)	1260, 1406, 3552
Eco88I	CYCGRG	3 cut(s)	516, 2276, 3437
EcoICRI	GAGCTC	1 cut(s)	2253
EcoO109I	RGGNCCY	1 cut(s)	2578
EcoRI	GAATTC	2 cut(s)	444, 3770
EcoRII	CCWGG	4 cut(s)	775, 2413, 2920, 3871
EcoT14I	CCWWGG	6 cut(s)	797, 1791, 2344, 2475, 3532, 3894
EcoT22I	ATGCAT	1 cut(s)	697
EcoT38I	GRGCYC	1 cut(s)	2255
ErhI	CCWWGG	6 cut(s)	797, 1791, 2344, 2475, 3532, 3894
FalI	AAGNNNNNCTT	8 cut(s)	790, 822, 987, 1019, 1093, 1125, 3345, 3377
FaqI	GGGAC	5 cut(s)	534, 581, 1414, 1773, 2068
FauNDI	CATATG	1 cut(s)	1992
FbaI	TGATCA	3 cut(s)	84, 973, 1933
FokI	GGATG	9 cut(s)	669, 983, 1093, 1537, 1624, 1869, 2557, 2985, 3231
FriOI	GRGCYC	1 cut(s)	2255
GlaI	GCGC	1 cut(s)	3435
GsuI	CTGGAG	7 cut(s)	714, 798, 2099, 2397, 2943, 3894, 3941
HaeII	RGCGCY	1 cut(s)	3437
HaeIII	GGCC	6 cut(s)	802, 1145, 2124, 2579, 2792, 3721
HapII	CCGG	2 cut(s)	475, 3385
HhaI	GCGC	1 cut(s)	3436
Hin6I	GCGC	1 cut(s)	3434
HinP1I	GCGC	1 cut(s)	3434
HincII	GTYRAC	2 cut(s)	2032, 2931
HindII	GTYRAC	2 cut(s)	2032, 2931
HindIII	AAGCTT	4 cut(s)	936, 1275, 2750, 3167
HpaII	CCGG	2 cut(s)	475, 3385
HphI	GGTGA	4 cut(s)	1941, 3134, 3686, 3788
Hpy166II	GTNNAC	5 cut(s)	26, 2032, 2931, 3257, 3677
Hpy8I	GTNNAC	5 cut(s)	26, 2032, 2931, 3257, 3677
Hpy99I	CGWCG	4 cut(s)	637, 2310, 2403, 3939
HpyAV	CCTTC	7 cut(s)	367, 397, 821, 2282, 3005, 3237, 3424
HpyCH4IV	ACGT	7 cut(s)	632, 1227, 1281, 1853, 2034, 2401, 3253
HpyF10VI	GCNNNNNNNGC	6 cut(s)	716, 1900, 2130, 2681, 2978, 3925
HpySE526I	ACGT	7 cut(s)	632, 1227, 1281, 1853, 2034, 2401, 3253
HspAI	GCGC	1 cut(s)	3434
Ksp22I	TGATCA	3 cut(s)	84, 973, 1933
LmnI	GCTCC	8 cut(s)	467, 777, 2206, 2258, 2416, 2588, 3146, 3875
MaeII	ACGT	7 cut(s)	632, 1227, 1281, 1853, 2034, 2401, 3253
MfeI	CAATTG	4 cut(s)	1689, 3216, 3239, 3765
MflI	RGATCY	9 cut(s)	124, 646, 1246, 1385, 2437, 2663, 2872, 3586, 3868
MhlI	GDGCHC	4 cut(s)	28, 1451, 2255, 2475
MlsI	TGGCCA	2 cut(s)	2124, 3721
MluNI	TGGCCA	2 cut(s)	2124, 3721
MlyI	GAGTC	9 cut(s)	140, 395, 728, 1795, 2090, 2855, 3043, 3581, 3634
MmeI	TCCRAC	3 cut(s)	2494, 2850, 3349
Mox20I	TGGCCA	2 cut(s)	2124, 3721
Mph1103I	ATGCAT	1 cut(s)	697
MroXI	GAANNNNTTC	4 cut(s)	96, 189, 1534, 3357
MscI	TGGCCA	2 cut(s)	2124, 3721
MslI	CAYNNNNRTG	4 cut(s)	1308, 1444, 2182, 2446
Msp20I	TGGCCA	2 cut(s)	2124, 3721
MspA1I	CMGCKG	2 cut(s)	2948, 3349
MspI	CCGG	2 cut(s)	475, 3385
MspR9I	CCNGG	4 cut(s)	777, 2415, 2922, 3873
MunI	CAATTG	4 cut(s)	1689, 3216, 3239, 3765
Mva1269I	GAATGC	1 cut(s)	1089
MvaI	CCWGG	4 cut(s)	777, 2415, 2922, 3873
MwoI	GCNNNNNNNGC	6 cut(s)	716, 1900, 2130, 2681, 2978, 3925
NcoI	CCATGG	2 cut(s)	3532, 3894
NdeI	CATATG	1 cut(s)	1992
NlaIV	GGNNCC	6 cut(s)	1387, 2590, 2665, 2824, 3108, 3877
NmuCI	GTSAC	7 cut(s)	256, 2534, 3035, 3122, 3220, 3482, 3626
NsiI	ATGCAT	1 cut(s)	697
NspI	RCATGY	2 cut(s)	2804, 3306
OliI	CACNNNNGTG	1 cut(s)	1444
PaeR7I	CTCGAG	1 cut(s)	3437
PaqCI	CACCTGC	1 cut(s)	1034
PceI	AGGCCT	1 cut(s)	1145
PciI	ACATGT	1 cut(s)	3302
PctI	GAATGC	1 cut(s)	1089
PdmI	GAANNNNTTC	4 cut(s)	96, 189, 1534, 3357
PflMI	CCANNNNNTGG	1 cut(s)	3650
PfoI	TCCNGGA	2 cut(s)	2920, 3871
PleI	GAGTC	9 cut(s)	139, 394, 728, 1794, 2089, 2855, 3042, 3580, 3633
PpsI	GAGTC	9 cut(s)	139, 394, 728, 1794, 2089, 2855, 3042, 3580, 3633
Ppu21I	YACGTR	1 cut(s)	3254
PscI	ACATGT	1 cut(s)	3302
Psp124BI	GAGCTC	1 cut(s)	2255
Psp6I	CCWGG	4 cut(s)	775, 2413, 2920, 3871
PspGI	CCWGG	4 cut(s)	775, 2413, 2920, 3871
PspN4I	GGNNCC	6 cut(s)	1387, 2590, 2665, 2824, 3108, 3877
PspPI	GGNCC	4 cut(s)	887, 2578, 3107, 3653
PstI	CTGCAG	3 cut(s)	1045, 3494, 3907
PstNI	CAGNNNCTG	4 cut(s)	1049, 2832, 2951, 3071
PsuI	RGATCY	9 cut(s)	124, 646, 1246, 1385, 2437, 2663, 2872, 3586, 3868
PvuII	CAGCTG	2 cut(s)	2948, 3349
RseI	CAYNNNNRTG	4 cut(s)	1308, 1444, 2182, 2446
SacI	GAGCTC	1 cut(s)	2255
Sau96I	GGNCC	4 cut(s)	887, 2578, 3107, 3653
ScaI	AGTACT	2 cut(s)	617, 1841
SchI	GAGTC	9 cut(s)	140, 395, 728, 1795, 2090, 2855, 3043, 3581, 3634
ScrFI	CCNGG	4 cut(s)	777, 2415, 2922, 3873
SduI	GDGCHC	4 cut(s)	28, 1451, 2255, 2475
SfcI	CTRYAG	3 cut(s)	1041, 3490, 3903
Sfr274I	CTCGAG	1 cut(s)	3437
SinI	GGWCC	3 cut(s)	887, 3107, 3653
SlaI	CTCGAG	1 cut(s)	3437
SmiMI	CAYNNNNRTG	4 cut(s)	1308, 1444, 2182, 2446
SmlI	CTYRAG	6 cut(s)	128, 1586, 1868, 2973, 3437, 3622
SmoI	CTYRAG	6 cut(s)	128, 1586, 1868, 2973, 3437, 3622
SnaBI	TACGTA	1 cut(s)	3254
SpeI	ACTAGT	3 cut(s)	880, 1201, 3322
SseBI	AGGCCT	1 cut(s)	1145
SsiI	CCGC	6 cut(s)	866, 1602, 1894, 2451, 2941, 3248
SstI	GAGCTC	1 cut(s)	2255
StuI	AGGCCT	1 cut(s)	1145
StyD4I	CCNGG	4 cut(s)	775, 2413, 2920, 3871
StyI	CCWWGG	6 cut(s)	797, 1791, 2344, 2475, 3532, 3894
TaiI	ACGT	7 cut(s)	635, 1230, 1284, 1856, 2037, 2404, 3256
TatI	WGTACW	4 cut(s)	615, 1839, 1864, 3305
TauI	GCSGC	1 cut(s)	869
TscAI	CASTG	9 cut(s)	94, 403, 430, 845, 1315, 2389, 3096, 3494, 3722
TseFI	GTSAC	7 cut(s)	256, 2534, 3035, 3122, 3220, 3482, 3626
Tsp45I	GTSAC	7 cut(s)	256, 2534, 3035, 3122, 3220, 3482, 3626
TspGWI	ACGGA	3 cut(s)	1378, 3390, 3952
TspRI	CASTG	9 cut(s)	94, 403, 430, 845, 1315, 2389, 3096, 3494, 3722
Van91I	CCANNNNNTGG	1 cut(s)	3650
VneI	GTGCAC	1 cut(s)	24
VpaK11BI	GGWCC	3 cut(s)	887, 3107, 3653
XbaI	TCTAGA	2 cut(s)	2161, 2426
XceI	RCATGY	2 cut(s)	2804, 3306
XhoI	CTCGAG	1 cut(s)	3437
XmaJI	CCTAGG	1 cut(s)	2344
XmnI	GAANNNNTTC	4 cut(s)	96, 189, 1534, 3357
ZrmI	AGTACT	2 cut(s)	617, 1841
Zsp2I	ATGCAT	1 cut(s)	697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.