Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001909

Physical Location & Seq

Genomic Coordinates Forward (+)

25441975 .. 25449561

Gene Structure 7587 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1163700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4797 bp

ATGGAGCCCACTGTTCTTAATCAGTTCATCCCTAGAAGGTACCAAGTGAAAGTGTTTGAGGTTGCAAAGCGGAAGAACACGATTGCGGTGCTGGAAACAGGAGCTGGGAAGACGATGATAGCTGTGATGCTCATCAAGCACGTTGCTGAGGAGATGATGAGTATCATCCCCTCCTCTGGAGTTAAAAAGTTGATCATTTTCTTGGCTCCGACAGTTTATCTTGTCACTCAACAATACAAGGTTATTAAAAATTCCACAACTCTTGAAGTGGAAGAGTACTATGGAGCCAAGGGAGTGGATGAATGGACCCGGAGCTGTTGGGAAACAGAAGCTAATACACATGATATACTGGTCATGACACCACAGGTTCTCTTGGATGCCATGAGGAACGCTTTCTTAAACTTGGAAATGTTTAGCTTGATAATCTTCGACGAATGTCATCGCGCTACAGGCAATCATCCCTATACGAAAATAATGAAGGATTTTTATCACCAATCTGTGAACCGGCCAAAGATTTTTGGGATGACAGCGTCCCCCGTTATAAGAAAAGGTGTCTCGTCCGCCATGGATTGTGAGGATCAAATATCTGCACTTGAAAGCATGTTGGATGCTCAGGTTTACACTGTAGAAGACAGGACTGAAATCGAAACGTTTGTTCCCTCAGCAGTCCACAAATGTAGGTTCTACAAGCAAGCACCGTCCACCTATTCGGATCTGAAGGAAAAGATGGAAGCTTTGCTGTCGAAGGTAGTCCTGCGCCGTATCATTTCGTTCGTTCACAAATATGCTTCTGAGTTGGAGTTGCAAGAGCAAATGCCAAGTCAGTACCAAGACATCAGTGACAAGATAAAGATGTTGCGAAAGCGGTTACACAATGACCACATGAAGATCTTGTACTGCCTTGATGACCTTGGCATTGCTGTCAAAGTTTGTCTAGAGAATGTCCCTGATGCCGAAGAAGAGTGCCAGTTCTATAGAGAATGTTCTTCGTATTTGAAAATTTTTCTCGATGAAGTATTGCTTATGATTGCCGGATTTCTTCAACCTGGCTGCGAACTTCATGCAAACTTTGAGTCTGACTATACAAAGGCGTGCGATTTGGGTTTCATATCTCCAAAGTTATACGAACTTCTTCAAATTTTCCAGTCATTTGGAACCGGTAGCAAAGTTCAATGCCTCATTTTCGTAGAGCGAATTATTACAGCGAAAGTGATTGAAATATATGTTAAGAAAGTGCGGTGCTTATCTCACTTGACGGTTTCATACGTGACTGGAAGTAACGGATCAGCTGATGCGCTATCACCGAAGCTGCAAAAGGAAACTATGGAATCCTTTTGCTCCGGGAAGGTCAATCTGTTGTTCGCTACGGATGTAGTTGAGGAGGGAATACATGTTGCAAACTGTTCCTGTGTGATTCGTTTTGACTTGCCCAAGACAGTTCGTAGTTATATCCAGTCCCGGGGAAGAGGTCGAAAAAATGACTCCCAATATATCCTAATGCTTGAAAGGAGCAACAAGAAGCAAATAGATCAAATGTTCGACATCATCAGGAGCGAGCATTCGATGACAGATACATCTAGAAACAGAGATCCTGAAGTGTGCACCTTAAAAGCTTGTTGTTTTGAGGAAACAAAATCGTATCGTGCAAAAGCTACTGGAGCATCAGTTACAACGGATTCAAGTGTCAGTCTTATATATCGATACTGCGAAAAGCTCCCGGGAGATAGGTACTTTACTCCGAAACCAACTTTTGACTTCGAAATTTCTGAAGGGTTGTACCAGTGTAGAATAACATTACCCCCAAATGCAGCTTTTCTAACAACGGTCGGTCCACTGAGCAGCAACACGCATTTGTCCAAGCAGCTAGTGTGCTTGGAAGCTTGTAAAAAACTCCATGAAATGGGAGCCTTGGATGATCATCTTCTCCCGCTTGTCCAAGAGCCCTTAGAAGATGATCTAATTCCAGAAAGAAGAGAGTTATCTGCTGGTGCAGGAACAACGAAAAGAAAGGAATTACACGGCACAACATCCATCCGGGCATTATGTGGAACTTGGGGAGAAAAACTTGATACTGTTTTTGAGGCCTATAAGTTTGATTTCTCGTGTAGTATCGTTGATGAGACATATTCAGGATTTGTTCTACTAATCGAGTCAAAACTCGATGATGATGTGGGAAATATCGAAATGGATCTTCAACTCGTCTCAAGGATGGTTAAATCTTCGGTCTCTTCATGTGGAAAATGTTATCTGAGTGCAGAGCAGGTAAGATTGCTATCACATTTCAATAGTTTCTTCTTGTTATTTAAACCACTTTCTGTAATTCTATGCTGCCTATTTTCTAACCAGGTAACGCAAGCCAAGCGCTTTCAAGAATTTTTCTTTAATGGTGTGTTTGGGAGATTGTTTTTTGGGGGAGAATCAGAAGGGACAACGCGAGAATTTTTACTCCAGACAGAAACAAAATCACTCTGGAACTCAGCATATATGTATTTGCTTTTACCCGTTGACACATTGGACACTTCAGAAGTGAACTGGAGAGGGATCAGTTCTTCTGTTAATGTGGTAGAATTTTTTAAGAAACAATACTCCACGCATTCCAAGTGTTTTAATGGTGATCACAGCGAGTTGCCACTTTCTAGGACTGGTTCATCCATGACAGAGTCGAAGGCTTCGAATCATATCCATTTCGCTAATAGTTCAATTGATGCCGATAATCTCCAAGATATGCTAGTATTGGCTATTCACACGGGACGTATCTATTCAATCATCGAAGTTGTCCATGGAAAATCTGCAGAAAGTTCTTTGGAGGAAAATATTGATGATGCATCATCGACATACAGTTATGCTCAGCACTTCAAAACGAAGTATGGGATTTCACTTGTTTATCCCGGACAGCCTTTGCTGCGGTTAAAGCAAAGTCATAATCCGCATAACTTGCTTGGTGCCGAAGGTGCTTCATCACAAGACGGTCGAGTAATCAAGGAACAAGCTCATGTTCGTATGCCTCCCGAGATCTTAGTCAGTATTGATTTTCAGGTGGATGTTTTACGATCATGTTACTTGTTACCGTCAGTAATGCACCGGCTGGAGTCACTAATGCTAGCTAGCCAGCTCAGGGAAGAGATCAATGGCCAAACTAACAACTTTAAAATATCAAGTTCATTGATTCTGGAAGCACTCACAACACTTAGGTGTTGTGAAGATTTCTCATTGGAACGTTTGGAGTTGCTCGGAGATTCAGTTCTCAAGTACGCTGTGAGCTCCTACCTTTTTCTCAGATATCCTGAGAAACATGAAGGACAACTAACAGCTCGGCGTACAAGGGTAATATGTAACTCCAACCTGCATAAACTAGGAATCCAACGAAAGATACAGGGATATATACGTGACAGCGCATTTGAACCACGCCGTTGGGCAGCTCCAGGGCAGCTGTCTAGATTCCCTGATCCGTGTAAATGCGGAGTGGACACCTTAGAAGTGCCTTTGGACAGCAGATTTCAAACTGTAGAAGCGGTAAAGGTCGGAAAATTCTGCGATAGTGGCCATAGATGGATGAACTCGAAAACCATAGCAGATTGTGTTGAGGCCCTCATAGGAGCCTACTATGTCGGCAAAGGACTGTTTGCTGCACTACATGTGATGAAGTGGCTTGGTATTGATTCTGATTTTGAACCCTCATTAGCCACTGAAGCCATTACGAGGGCTTCCCTCCGATCTTACTACCCAAAACATGATGACATCGCTGCCTTGGAGTTGAAGCTTGACTACCAATTTTCGGTTAAGGGTTTGTTACAGGAGGCCATAACACATGCCTCTGAGCAAAAACTAGGTGCTAGCTACTGTTACCAGAGGCTCGAGTTTCTTGGCGACTCTGTGTTGGATGTGCTAATCACACAGTATCTCTATGATAGCCACACAACTATTAATCCGGGATGGTTGACAGACTTGCGATCAGCTGCCGTTTGTAACGAAAACTTTGCCCGAGCTGCCGTGAGAAAGAAACTTCACCCACATCTTCAACATTGCTCCGGGTTACTTCTGAATCATATCACAGAGTATGAGAAGTTGTGTACTGCTGAAGCTCTTAACACCACCAGCCTACTTGAAGACGTAAAAGGCCCTAAAGCTCTTGGGGACATGGTAGAAAGCATAGCTGGGGCTATATACATGGACAGCAATCTCGATCTTAACAAAGTATGGAGGGTGTTTAAACCGCTATTATCTCCGATCGTGACCCCTTCCACTCTGCAATTACATCCGCTGCGTGAACTGACCGAGTTCTGTGGTTCTCTCGGGTACTTTGTGAAAGAAAATTGTACTAAAGAGGATTCATTGGAGCATGTGGAAATGACGTTGCAGCTGGAAGATGGTCTTTTGGTTGGTAATGGGTACGATCGGAGCAAGAAAGCTGCTAAACAGAAAGCAGCTCGTCAACTGTTGAAGGAGCTCCAGGGTTGCCATTCAGTGTTTAAAAGGAGAAAACTTGATCCTGACCGTGTAGATGAGCTCTCTTCCGAAGGCATGGAGATTGAACACTGCAGCCAACTGAATGATACTCATTCTTTAGAGTCGATCAATCGAATCAAATCACCATATGCAGACTCGAGAACAGGTCCTACCCCGACCAATTTTGGTCCTAACAAAATCTACAGTCACAACATTGTAACCCCAAGTTTACATGCTATAGCTTCGATCAGCACGAGGAAAGGAGCAGCGCGGGCTTCTCTCTTTGAGCTGTGTAAGAGAATGCAGTGGCCAATGCCTACATTCAAAACAACAGAGCATAAATCGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1599

Amino Acids

180.5

Weight (kDa)

6.46

Isoelectric Point (pI)

43.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	12 - 179	7.4e-18	Type III restriction enzyme, res subunit
DEAD	PF00270	13 - 179	5.9e-18	DEAD/DEAH box helicase
Helicase_C	PF00271	381 - 489	7.1e-16	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	561 - 647	3.2e-24	Dicer dimerisation domain
PAZ	PF02170	902 - 1006	5.1e-08	PAZ domain
Ribonuclease_3	PF00636	1072 - 1205	8.3e-21	Ribonuclease III domain
Ribonucleas_3_3	PF14622	1072 - 1129	5.2e-07	Ribonuclease-III-like
Ribonucleas_3_3	PF14622	1262 - 1393	1.7e-13	Ribonuclease-III-like
Ribonuclease_3	PF00636	1283 - 1392	9.5e-21	Ribonuclease III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001909)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920 AT3G43920
fragaria_vesca	FvH4_2g07790 FvH4_2g18930 FvH4_2g18930 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780 FvH4_2g30780
malus_domestica	MD08G1046300.v1.1 MD10G1163700.v1.1 MD15G1047500.v1.1
prunus_persica	Prupe.1G401900_v2.0.a1 Prupe.1G401900_v2.0.a1 Prupe.8G202000_v2.0.a1 Prupe.8G202000_v2.0.a1
pyrus_communis	pycom08g03830 pycom08g03840 pycom15g04670
rosa_chinensis	RchiOBHm_Chr6g0284721 RchiOBHm_Chr6g0312121
rosa_laevigata	RLG00000010353 RLG00000012724
rosa_multiflora	Rmu_sc0004065.1_g000007 Rmu_sc0007474.1_g000021
rosa_roxburghii	Rroxscaffold_7G00157050 Rroxscaffold_7G00184440
rosa_rugosa	Rorug06G0165900 Rorug06G0392600 Rorug06G0392700 Rorug06G0392800
rosa_samantha	Rh4CG118000 Rh6AG279000 Rh6AG509700 Rh6BG279000 Rh6BG520600 Rh6CG281300 Rh6CG526600 Rh6DG274000 Rh6DG512000
rosa_wichuraiana	Rw6G023980 Rw6G044350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	542
Acc36I	ACCTGC	2 cut(s)	2251, 3351
Acc65I	GGTACC	1 cut(s)	39
AccB1I	GGYRCC	2 cut(s)	39, 2942
AccB7I	CCANNNNNTGG	1 cut(s)	1900
AccII	CGCG	3 cut(s)	444, 2434, 4717
AclI	AACGTT	2 cut(s)	650, 3217
AclWI	GGATC	8 cut(s)	585, 720, 1291, 1583, 2196, 2549, 3440, 4481
AcoI	YGGCCR	4 cut(s)	506, 3130, 3541, 4754
AcsI	RAATTY	8 cut(s)	250, 999, 1137, 1761, 2372, 2438, 2567, 3527
AcuI	CTGAAG	6 cut(s)	737, 1614, 1788, 2505, 3708, 4098
AfeI	AGCGCT	1 cut(s)	2363
AfiI	CCNNNNNNNGG	6 cut(s)	176, 1459, 1900, 3115, 3699, 3775
AflIII	ACRYGT	2 cut(s)	1390, 3634
AgeI	ACCGGT	1 cut(s)	1157
AjnI	CCWGG	4 cut(s)	1045, 2343, 3421, 4449
AleI	CACNNNNGTG	1 cut(s)	3190
AloI	GAACNNNNNNTCC	2 cut(s)	3225, 3257
Alw21I	GWGCWC	4 cut(s)	1604, 3263, 4449, 4509
Alw26I	GTCTC	4 cut(s)	559, 2114, 2206, 2230
Alw44I	GTGCAC	1 cut(s)	1600
AlwI	GGATC	8 cut(s)	585, 720, 1291, 1583, 2196, 2549, 3440, 4481
AlwNI	CAGNNNCTG	1 cut(s)	104
Ama87I	CYCGRG	7 cut(s)	1458, 1717, 3008, 3854, 3981, 4292, 4603
Aor51HI	AGCGCT	1 cut(s)	2363
AoxI	GGCC	8 cut(s)	506, 2082, 3130, 3541, 3585, 3798, 4117, 4754
ApaLI	GTGCAC	1 cut(s)	1600
ApoI	RAATTY	8 cut(s)	250, 999, 1137, 1761, 2372, 2438, 2567, 3527
ArsI	GACNNNNNNTTYG	6 cut(s)	805, 837, 1058, 1090, 3380, 3412
AseI	ATTAAT	1 cut(s)	3924
AsiGI	ACCGGT	1 cut(s)	1157
Asp700I	GAANNNNTTC	2 cut(s)	392, 1131
Asp718I	GGTACC	1 cut(s)	39
AspLEI	GCGC	6 cut(s)	446, 759, 1297, 2364, 3395, 4717
AspS9I	GGNCC	6 cut(s)	306, 1829, 3586, 4118, 4613, 4634
AsuHPI	GGTGA	5 cut(s)	482, 1293, 2624, 3998, 4581
AsuII	TTCGAA	2 cut(s)	1758, 2672
AsuNHI	GCTAGC	3 cut(s)	3100, 3104, 3833
AvaI	CYCGRG	7 cut(s)	1458, 1717, 3008, 3854, 3981, 4292, 4603
AvaII	GGWCC	4 cut(s)	306, 1829, 4613, 4634
BaeGI	GKGCMC	1 cut(s)	1604
BalI	TGGCCA	3 cut(s)	3132, 3543, 4756
BanI	GGYRCC	2 cut(s)	39, 2942
BanII	GRGCYC	5 cut(s)	9, 1944, 3263, 4449, 4509
BarI	GAAGNNNNNNTAC	4 cut(s)	878, 910, 3749, 3781
BauI	CACGAG	2 cut(s)	2101, 4699
BbsI	GAAGAC	3 cut(s)	116, 636, 4113
Bbv12I	GWGCWC	4 cut(s)	1604, 3263, 4449, 4509
BbvCI	CCTCAGC	2 cut(s)	147, 661
BccI	CCATC	6 cut(s)	721, 2039, 2203, 3543, 3927, 4361
BceAI	ACGGC	5 cut(s)	744, 2035, 3393, 3944, 3974
BcgI	CGANNNNNNTGC	6 cut(s)	70, 104, 1043, 1077, 3959, 3993
BciT130I	CCWGG	4 cut(s)	1047, 2345, 3423, 4451
BclI	TGATCA	3 cut(s)	192, 1915, 2614
BcoDI	GTCTC	4 cut(s)	559, 2114, 2206, 2230
BfmI	CTRYAG	8 cut(s)	447, 624, 973, 2790, 3504, 4537, 4648, 4683
BfoI	RGCGCY	1 cut(s)	2365
BfuAI	ACCTGC	2 cut(s)	2251, 3351
BglII	AGATCT	2 cut(s)	888, 3012
BlpI	GCTNAGC	1 cut(s)	2847
BmcAI	AGTACT	1 cut(s)	278
Bme18I	GGWCC	4 cut(s)	306, 1829, 4613, 4634
BmeT110I	CYCGRG	7 cut(s)	1458, 1717, 3008, 3854, 3981, 4292, 4603
BmgT120I	GGNCC	6 cut(s)	306, 1829, 3586, 4118, 4613, 4634
BmiI	GGNNCC	9 cut(s)	6, 41, 207, 286, 308, 1156, 1906, 2944, 3598
BmsI	GCATC	9 cut(s)	117, 367, 598, 940, 1282, 1670, 2695, 2812, 2834
BmtI	GCTAGC	3 cut(s)	3104, 3108, 3837
BoxI	GACNNNNGTC	1 cut(s)	435
BpiI	GAAGAC	3 cut(s)	116, 636, 4113
BpmI	CTGGAG	7 cut(s)	198, 1677, 2432, 2554, 3107, 3405, 4433
Bpu10I	CCTNAGC	4 cut(s)	147, 612, 661, 3113
Bpu1102I	GCTNAGC	1 cut(s)	2847
Bpu14I	TTCGAA	2 cut(s)	1758, 2672
BpuEI	CTTGAG	2 cut(s)	2188, 3230
Bsa29I	ATCGAT	1 cut(s)	1699
BsaAI	YACGTR	2 cut(s)	1267, 3386
BsaBI	GATNNNNATC	5 cut(s)	131, 161, 486, 1917, 2272
BsaI	GGTCTC	1 cut(s)	2230
BsaWI	WCCGGW	1 cut(s)	1157
BsaXI	ACNNNNNCTCC	4 cut(s)	1650, 1680, 3225, 3255
Bsc4I	CCNNNNNNNGG	6 cut(s)	176, 1459, 1900, 3115, 3699, 3775
Bse118I	RCCGGY	3 cut(s)	504, 1157, 3081
Bse1I	ACTGG	9 cut(s)	354, 967, 1144, 1276, 1453, 1660, 1780, 2537, 2647
Bse3DI	GCAATG	2 cut(s)	915, 4021
Bse8I	GATNNNNATC	5 cut(s)	131, 161, 486, 1917, 2272
BseBI	CCWGG	4 cut(s)	1047, 2345, 3423, 4451
BseCI	ATCGAT	1 cut(s)	1699
BseJI	GATNNNNATC	5 cut(s)	131, 161, 486, 1917, 2272
BseLI	CCNNNNNNNGG	6 cut(s)	176, 1459, 1900, 3115, 3699, 3775
BseMI	GCAATG	2 cut(s)	915, 4021
BseNI	ACTGG	9 cut(s)	354, 967, 1144, 1276, 1453, 1660, 1780, 2537, 2647
BseRI	GAGGAG	3 cut(s)	163, 164, 1394
BseSI	GKGCMC	1 cut(s)	1604
BseYI	CCCAGC	2 cut(s)	104, 4154
BsgI	GTGCAG	4 cut(s)	573, 2010, 2274, 3612
Bsh1236I	CGCG	3 cut(s)	444, 2434, 4717
Bsh1285I	CGRYCG	4 cut(s)	1827, 2971, 4230, 4396
BshFI	GGCC	8 cut(s)	508, 2084, 3132, 3543, 3587, 3800, 4119, 4756
BshNI	GGYRCC	2 cut(s)	39, 2942
BshTI	ACCGGT	1 cut(s)	1157
BshVI	ATCGAT	1 cut(s)	1699
BsiEI	CGRYCG	4 cut(s)	1827, 2971, 4230, 4396
BsiHKAI	GWGCWC	4 cut(s)	1604, 3263, 4449, 4509
BsiHKCI	CYCGRG	7 cut(s)	1458, 1717, 3008, 3854, 3981, 4292, 4603
BslFI	GGGAC	6 cut(s)	517, 929, 1441, 2440, 2763, 4148
BslI	CCNNNNNNNGG	6 cut(s)	176, 1459, 1900, 3115, 3699, 3775
BsmAI	GTCTC	4 cut(s)	559, 2114, 2206, 2230
BsmBI	CGTCTC	1 cut(s)	2206
BsmFI	GGGAC	6 cut(s)	517, 929, 1441, 2440, 2763, 4148
BsmI	GAATGC	3 cut(s)	1558, 2593, 4752
BsnI	GGCC	8 cut(s)	508, 2084, 3132, 3543, 3587, 3800, 4119, 4756
Bso31I	GGTCTC	1 cut(s)	2230
BsoBI	CYCGRG	7 cut(s)	1458, 1717, 3008, 3854, 3981, 4292, 4603
Bsp119I	TTCGAA	2 cut(s)	1758, 2672
Bsp1286I	GDGCHC	6 cut(s)	9, 1604, 1944, 3263, 4449, 4509
Bsp1720I	GCTNAGC	1 cut(s)	2847
Bsp19I	CCATGG	2 cut(s)	564, 2779
BspANI	GGCC	8 cut(s)	508, 2084, 3132, 3543, 3587, 3800, 4119, 4756
BspDI	ATCGAT	1 cut(s)	1699
BspFNI	CGCG	3 cut(s)	444, 2434, 4717
BspHI	TCATGA	1 cut(s)	354
BspLI	GGNNCC	9 cut(s)	6, 41, 207, 286, 308, 1156, 1906, 2944, 3598
BspMAI	CTGCAG	2 cut(s)	2794, 4541
BspMI	ACCTGC	2 cut(s)	2251, 3351
BspOI	GCTAGC	3 cut(s)	3104, 3108, 3837
BspPI	GGATC	8 cut(s)	585, 720, 1291, 1583, 2196, 2549, 3440, 4481
BspT104I	TTCGAA	2 cut(s)	1758, 2672
BspT107I	GGYRCC	2 cut(s)	39, 2942
BspTNI	GGTCTC	1 cut(s)	2230
BsrDI	GCAATG	2 cut(s)	915, 4021
BsrFI	RCCGGY	3 cut(s)	504, 1157, 3081
BsrI	ACTGG	9 cut(s)	354, 967, 1144, 1276, 1453, 1660, 1780, 2537, 2647
BssAI	RCCGGY	3 cut(s)	504, 1157, 3081
BssSI	CACGAG	2 cut(s)	2101, 4699
BssT1I	CCWWGG	6 cut(s)	288, 564, 910, 1908, 2779, 3747
Bst2BI	CACGAG	2 cut(s)	2101, 4699
Bst2UI	CCWGG	4 cut(s)	1047, 2345, 3423, 4451
Bst6I	CTCTTC	7 cut(s)	267, 954, 1459, 1966, 2233, 3114, 4516
BstAPI	GCANNNNNTGC	1 cut(s)	2935
BstBAI	YACGTR	2 cut(s)	1267, 3386
BstBI	TTCGAA	2 cut(s)	1758, 2672
BstC8I	GCNNGC	9 cut(s)	693, 1093, 1556, 2355, 3102, 3106, 3110, 3835, 4719
BstDSI	CCRYGG	2 cut(s)	564, 2779
BstFNI	CGCG	3 cut(s)	444, 2434, 4717
BstH2I	RGCGCY	1 cut(s)	2365
BstHHI	GCGC	6 cut(s)	446, 759, 1297, 2364, 3395, 4717
BstMAI	GTCTC	4 cut(s)	559, 2114, 2206, 2230
BstMCI	CGRYCG	4 cut(s)	1827, 2971, 4230, 4396
BstNI	CCWGG	4 cut(s)	1047, 2345, 3423, 4451
BstNSI	RCATGY	6 cut(s)	604, 1394, 3638, 3812, 4343, 4682
BstPAI	GACNNNNGTC	1 cut(s)	435
BstSFI	CTRYAG	8 cut(s)	447, 624, 973, 2790, 3504, 4537, 4648, 4683
BstSLI	GKGCMC	1 cut(s)	1604
BstUI	CGCG	3 cut(s)	444, 2434, 4717
BstV2I	GAAGAC	3 cut(s)	116, 636, 4113
BstX2I	RGATCY	5 cut(s)	712, 888, 1588, 2188, 3012
BstXI	CCANNNNNNTGG	3 cut(s)	295, 1151, 3411
BstYI	RGATCY	5 cut(s)	712, 888, 1588, 2188, 3012
Bsu15I	ATCGAT	1 cut(s)	1699
BsuRI	GGCC	8 cut(s)	508, 2084, 3132, 3543, 3587, 3800, 4119, 4756
BsuTUI	ATCGAT	1 cut(s)	1699
BtgI	CCRYGG	2 cut(s)	564, 2779
BtgZI	GCGATG	2 cut(s)	425, 3724
BtsI	GCAGTG	2 cut(s)	4534, 4757
BtsIMutI	CAGTG	9 cut(s)	9, 621, 844, 1787, 1832, 3684, 4470, 4534, 4757
BveI	ACCTGC	2 cut(s)	2251, 3351
Cac8I	GCNNGC	9 cut(s)	693, 1093, 1556, 2355, 3102, 3106, 3110, 3835, 4719
CaiI	CAGNNNCTG	1 cut(s)	104
CciI	TCATGA	1 cut(s)	354
CfoI	GCGC	6 cut(s)	446, 759, 1297, 2364, 3395, 4717
Cfr10I	RCCGGY	3 cut(s)	504, 1157, 3081
Cfr13I	GGNCC	6 cut(s)	306, 1829, 3586, 4118, 4613, 4634
Cfr9I	CCCGGG	2 cut(s)	1458, 1717
ClaI	ATCGAT	1 cut(s)	1699
CseI	GACGC	1 cut(s)	519
CsiI	ACCWGGT	1 cut(s)	2343
CspAI	ACCGGT	1 cut(s)	1157
CspCI	CAANNNNNGTGG	2 cut(s)	244, 279
DraI	TTTAAA	4 cut(s)	2305, 3148, 4210, 4471
EaeI	YGGCCR	4 cut(s)	506, 3130, 3541, 4754
Eam1104I	CTCTTC	7 cut(s)	267, 954, 1459, 1966, 2233, 3114, 4516
EarI	CTCTTC	7 cut(s)	267, 954, 1459, 1966, 2233, 3114, 4516
EciI	GGCGGA	1 cut(s)	550
Ecl136II	GAGCTC	3 cut(s)	3261, 4447, 4507
Eco130I	CCWWGG	6 cut(s)	288, 564, 910, 1908, 2779, 3747
Eco147I	AGGCCT	1 cut(s)	2084
Eco24I	GRGCYC	5 cut(s)	9, 1944, 3263, 4449, 4509
Eco31I	GGTCTC	1 cut(s)	2230
Eco32I	GATATC	1 cut(s)	3281
Eco47I	GGWCC	4 cut(s)	306, 1829, 4613, 4634
Eco47III	AGCGCT	1 cut(s)	2363
Eco53kI	GAGCTC	3 cut(s)	3261, 4447, 4507
Eco57I	CTGAAG	6 cut(s)	737, 1614, 1788, 2505, 3708, 4098
Eco88I	CYCGRG	7 cut(s)	1458, 1717, 3008, 3854, 3981, 4292, 4603
EcoICRI	GAGCTC	3 cut(s)	3261, 4447, 4507
EcoO109I	RGGNCCY	3 cut(s)	3586, 4118, 4613
EcoRII	CCWGG	4 cut(s)	1045, 2343, 3421, 4449
EcoRV	GATATC	1 cut(s)	3281
EcoT14I	CCWWGG	6 cut(s)	288, 564, 910, 1908, 2779, 3747
EcoT22I	ATGCAT	1 cut(s)	2827
EcoT38I	GRGCYC	5 cut(s)	9, 1944, 3263, 4449, 4509
ErhI	CCWWGG	6 cut(s)	288, 564, 910, 1908, 2779, 3747
Esp3I	CGTCTC	1 cut(s)	2206
FalI	AAGNNNNNCTT	4 cut(s)	1005, 1037, 4356, 4388
FaqI	GGGAC	6 cut(s)	517, 929, 1441, 2440, 2763, 4148
FauI	CCCGC	2 cut(s)	1935, 4710
FauNDI	CATATG	1 cut(s)	4594
FbaI	TGATCA	3 cut(s)	192, 1915, 2614
FriOI	GRGCYC	5 cut(s)	9, 1944, 3263, 4449, 4509
GlaI	GCGC	6 cut(s)	445, 758, 1296, 2363, 3394, 4716
GsaI	CCCAGC	2 cut(s)	108, 4158
GsuI	CTGGAG	7 cut(s)	198, 1677, 2432, 2554, 3107, 3405, 4433
HaeII	RGCGCY	1 cut(s)	2365
HaeIII	GGCC	8 cut(s)	508, 2084, 3132, 3543, 3587, 3800, 4119, 4756
HgaI	GACGC	1 cut(s)	519
HhaI	GCGC	6 cut(s)	446, 759, 1297, 2364, 3395, 4717
Hin6I	GCGC	6 cut(s)	444, 757, 1295, 2362, 3393, 4715
HinP1I	GCGC	6 cut(s)	444, 757, 1295, 2362, 3393, 4715
HincII	GTYRAC	3 cut(s)	2506, 3939, 4433
HindII	GTYRAC	3 cut(s)	2506, 3939, 4433
HindIII	AAGCTT	4 cut(s)	732, 1611, 1878, 3758
HphI	GGTGA	5 cut(s)	482, 1293, 2624, 3998, 4581
Hpy99I	CGWCG	1 cut(s)	434
HpyCH4IV	ACGT	8 cut(s)	141, 650, 1266, 2752, 3217, 3385, 4110, 4352
HpySE526I	ACGT	8 cut(s)	141, 650, 1266, 2752, 3217, 3385, 4110, 4352
HspAI	GCGC	6 cut(s)	444, 757, 1295, 2362, 3393, 4715
KpnI	GGTACC	1 cut(s)	43
Ksp22I	TGATCA	3 cut(s)	192, 1915, 2614
LweI	GCATC	9 cut(s)	117, 367, 598, 940, 1282, 1670, 2695, 2812, 2834
MabI	ACCWGGT	1 cut(s)	2343
MaeII	ACGT	8 cut(s)	141, 650, 1266, 2752, 3217, 3385, 4110, 4352
MfeI	CAATTG	1 cut(s)	2700
MflI	RGATCY	5 cut(s)	712, 888, 1588, 2188, 3012
MhlI	GDGCHC	6 cut(s)	9, 1604, 1944, 3263, 4449, 4509
MlsI	TGGCCA	3 cut(s)	3132, 3543, 4756
MluNI	TGGCCA	3 cut(s)	3132, 3543, 4756
MlyI	GAGTC	8 cut(s)	1082, 1475, 2159, 2669, 3098, 3863, 4577, 4595
MmeI	TCCRAC	7 cut(s)	233, 585, 777, 3363, 3385, 3502, 3858
Mox20I	TGGCCA	3 cut(s)	3132, 3543, 4756
Mph1103I	ATGCAT	1 cut(s)	2827
MroXI	GAANNNNTTC	2 cut(s)	392, 1131
MscI	TGGCCA	3 cut(s)	3132, 3543, 4756
MslI	CAYNNNNRTG	2 cut(s)	783, 3190
Msp20I	TGGCCA	3 cut(s)	3132, 3543, 4756
MspA1I	CMGCKG	5 cut(s)	1289, 3430, 3956, 4261, 4360
MssI	GTTTAAAC	1 cut(s)	4210
MunI	CAATTG	1 cut(s)	2700
Mva1269I	GAATGC	3 cut(s)	1558, 2593, 4752
MvaI	CCWGG	4 cut(s)	1047, 2345, 3423, 4451
MvnI	CGCG	3 cut(s)	444, 2434, 4717
NcoI	CCATGG	2 cut(s)	564, 2779
NdeI	CATATG	1 cut(s)	4594
NheI	GCTAGC	3 cut(s)	3100, 3104, 3833
NlaIV	GGNNCC	9 cut(s)	6, 41, 207, 286, 308, 1156, 1906, 2944, 3598
NmeAIII	GCCGAG	1 cut(s)	3292
NmuCI	GTSAC	7 cut(s)	223, 839, 1267, 3090, 3386, 4231, 4652
NsiI	ATGCAT	1 cut(s)	2827
NspI	RCATGY	6 cut(s)	604, 1394, 3638, 3812, 4343, 4682
NspV	TTCGAA	2 cut(s)	1758, 2672
OliI	CACNNNNGTG	1 cut(s)	3190
PaeR7I	CTCGAG	2 cut(s)	3854, 4603
PagI	TCATGA	1 cut(s)	354
PceI	AGGCCT	1 cut(s)	2084
PciI	ACATGT	2 cut(s)	1390, 3634
PcsI	WCGNNNNNNNCGW	2 cut(s)	2669, 4697
PctI	GAATGC	3 cut(s)	1558, 2593, 4752
PdmI	GAANNNNTTC	2 cut(s)	392, 1131
PflFI	GACNNNGTC	2 cut(s)	529, 2659
PflMI	CCANNNNNTGG	1 cut(s)	1900
PfoI	TCCNGGA	3 cut(s)	1340, 2887, 3928
PinAI	ACCGGT	1 cut(s)	1157
Ple19I	CGATCG	2 cut(s)	4230, 4396
PleI	GAGTC	8 cut(s)	1081, 1475, 2158, 2668, 3097, 3863, 4576, 4595
PmeI	GTTTAAAC	1 cut(s)	4210
PpsI	GAGTC	8 cut(s)	1081, 1475, 2158, 2668, 3097, 3863, 4576, 4595
Ppu21I	YACGTR	2 cut(s)	1267, 3386
PpuMI	RGGWCCY	1 cut(s)	4613
PscI	ACATGT	2 cut(s)	1390, 3634
PshAI	GACNNNNGTC	1 cut(s)	435
PshBI	ATTAAT	1 cut(s)	3924
PsiI	TTATAA	1 cut(s)	542
Psp124BI	GAGCTC	3 cut(s)	3263, 4449, 4509
Psp1406I	AACGTT	2 cut(s)	650, 3217
Psp5II	RGGWCCY	1 cut(s)	4613
Psp6I	CCWGG	4 cut(s)	1045, 2343, 3421, 4449
PspFI	CCCAGC	2 cut(s)	104, 4154
PspGI	CCWGG	4 cut(s)	1045, 2343, 3421, 4449
PspN4I	GGNNCC	9 cut(s)	6, 41, 207, 286, 308, 1156, 1906, 2944, 3598
PspPI	GGNCC	6 cut(s)	306, 1829, 3586, 4118, 4613, 4634
PspPPI	RGGWCCY	1 cut(s)	4613
PspXI	VCTCGAGB	1 cut(s)	3854
PsrI	GAACNNNNNNTAC	2 cut(s)	4600, 4632
PstI	CTGCAG	2 cut(s)	2794, 4541
PstNI	CAGNNNCTG	1 cut(s)	104
PsuI	RGATCY	5 cut(s)	712, 888, 1588, 2188, 3012
PsyI	GACNNNGTC	2 cut(s)	529, 2659
PvuI	CGATCG	2 cut(s)	4230, 4396
PvuII	CAGCTG	4 cut(s)	1289, 3430, 3956, 4360
RseI	CAYNNNNRTG	2 cut(s)	783, 3190
SacI	GAGCTC	3 cut(s)	3263, 4449, 4509
Sau96I	GGNCC	6 cut(s)	306, 1829, 3586, 4118, 4613, 4634
ScaI	AGTACT	1 cut(s)	278
SchI	GAGTC	8 cut(s)	1082, 1475, 2159, 2669, 3098, 3863, 4577, 4595
SduI	GDGCHC	6 cut(s)	9, 1604, 1944, 3263, 4449, 4509
SexAI	ACCWGGT	1 cut(s)	2343
SfaNI	GCATC	9 cut(s)	117, 367, 598, 940, 1282, 1670, 2695, 2812, 2834
SfcI	CTRYAG	8 cut(s)	447, 624, 973, 2790, 3504, 4537, 4648, 4683
Sfr274I	CTCGAG	2 cut(s)	3854, 4603
SfuI	TTCGAA	2 cut(s)	1758, 2672
SinI	GGWCC	4 cut(s)	306, 1829, 4613, 4634
SlaI	CTCGAG	2 cut(s)	3854, 4603
SmaI	CCCGGG	2 cut(s)	1460, 1719
SmiMI	CAYNNNNRTG	2 cut(s)	783, 3190
SmlI	CTYRAG	4 cut(s)	2203, 3245, 3854, 4603
SmoI	CTYRAG	4 cut(s)	2203, 3245, 3854, 4603
SseBI	AGGCCT	1 cut(s)	2084
SspI	AATATT	1 cut(s)	2815
SstI	GAGCTC	3 cut(s)	3263, 4449, 4509
StuI	AGGCCT	1 cut(s)	2084
StyI	CCWWGG	6 cut(s)	288, 564, 910, 1908, 2779, 3747
TaiI	ACGT	8 cut(s)	144, 653, 1269, 2755, 3220, 3388, 4113, 4355
TaqII	GACCGA	3 cut(s)	1817, 2212, 4289
TatI	WGTACW	4 cut(s)	276, 894, 4070, 4316
TscAI	CASTG	9 cut(s)	16, 628, 844, 1787, 1839, 3691, 4470, 4541, 4757
TseFI	GTSAC	7 cut(s)	223, 839, 1267, 3090, 3386, 4231, 4652
Tsp45I	GTSAC	7 cut(s)	223, 839, 1267, 3090, 3386, 4231, 4652
TspGWI	ACGGA	4 cut(s)	1296, 1382, 1688, 3438
TspMI	CCCGGG	2 cut(s)	1458, 1717
TspRI	CASTG	9 cut(s)	16, 628, 844, 1787, 1839, 3691, 4470, 4541, 4757
Tth111I	GACNNNGTC	2 cut(s)	529, 2659
Van91I	CCANNNNNTGG	1 cut(s)	1900
VneI	GTGCAC	1 cut(s)	1600
VpaK11BI	GGWCC	4 cut(s)	306, 1829, 4613, 4634
VspI	ATTAAT	1 cut(s)	3924
XapI	RAATTY	8 cut(s)	250, 999, 1137, 1761, 2372, 2438, 2567, 3527
XbaI	TCTAGA	3 cut(s)	934, 1577, 3434
XceI	RCATGY	6 cut(s)	604, 1394, 3638, 3812, 4343, 4682
XhoI	CTCGAG	2 cut(s)	3854, 4603
XmaI	CCCGGG	2 cut(s)	1458, 1717
XmnI	GAANNNNTTC	2 cut(s)	392, 1131
ZrmI	AGTACT	1 cut(s)	278
Zsp2I	ATGCAT	1 cut(s)	2827

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.