Rroxscaffold_7G00157050

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001909
Physical Location & Seq
Reverse (-)
887187 .. 898162
10976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00157050.1

Sequence Viewer

Length: 3114 bp
ATGCATTTGCGAGAGGAAGAAGGCCAGAATTCCCCGAAACGCAGCTTCACTGAAACAACTGATGATCATCGCGCCGCCGCCGATCAAGGAGTTTCGAAGCGCCTCAATCCCAGAAGGTATCAGACACAAGTGTATGAGGTTGCTAGGAAGAGGAACACAATAGCTGTGATGGAGACAGGTACTGGCAAGACTATGATAGCTGTGATGCTCATTGATGAAATTGGTCAAGCCATCAAGTCATCTGGTGACAACAAATTGATCATATTCTTAGCCCCAACTGTTCATCTTGTTCATCAGCAATCTGAGGTTATTAAAGAACACACTAGTTTTAAAGTAGAGGAGTATTATGGAGCCAAGGGGGTTGATGCGTGGGATAAGGAGCATTGGGAAAAGGAATTTAATACCCATGATGTACTGGTTATGACACCCCAGATTCTTTTGGATGCCTTAAGAAAGGCATTCTTGACCATAGAAGCAGTATGCTTGATGATTATGGATGAGTGCCATCGGGCTACCGGAAACCACCCTTATACAAAGATAATGAAGGAGTTTTATCACAGGTCTAGTATCAAGCCAAAAGTTTTTGGAATGACAGCTTCTCCTGTAATTCGAAAGGGTGTCTCATCCACAATGGACTGTGAAGATCAAATATCTGAACTTGAAAGCATTTTGGATTCCCAGATTTATACTATAGAGGATAAAACAGAGATGGAAGTATATGCCCCTACTGCAAAAGAAACTCGTAGATTTTATAACCCAACATGGTTTTCGAGTTTGGATTTGAAAAAAAAGATCAAGGCCTCTTGGTCGAAGAATGATGCTTTGTTTTTAAAGCTGCAAGAATCAGTTCAAAGCAATTTCAAAGATTTGGATGACAAAGTTAAGGCCCTGCGGAAACGATTGTCTAATGACTATGAAAAGATCCTGTATTGCCTGGACGACCTTGGTCTCATATGTGCTTATGAGGCTGTGAAGGTCTGTCTAGAGAATGCTCCTACTAGCGAAGAGTGTGAATTTTATAGAGAAAGTTCTTTGCAGTGCAGATATTTTCTTGAGGAAGTTTTTGGTACAATCAAGCAATCTCTTTCTAATGGTTGTGAAAATTTTTTGGATGTTGGGTTTGACTATTTGAAGGCAGTAGATTTGGGCTACATATCTCCAAAGTTAAATGAACTTGTTCAGCTTTTCCATTCGTTTGGAGTATCTAGGGAACTACTGTGCCTCATCTTTGTGGATAGAATTGTCACTGCTAAAGTCATTGAAAGATTTATGAGAAAAGTTACCTCCTTATCTCATTTCAAAATTTCCTATTTGACTGGGAGTACTACATCAGTTGATGCTCTGACACCAAAAGTACAAAAGGAGACATTGAAGTTATTCAGCTCTGGGGAGGTCAATTTATTATTTTCTACCGATGTAGTTGAGGAGGGAATTCATGTTCCAAACTGCTCCTGTGTGATACGTTTTGACTTGCCAAAGACAGTGCGTAGTTATGTCCAGTCCAGAGGACGGGCTCGTCAAGATAACTCTCAATTCATTATAATGCTAGAAAGGGGAAACAAGCAACAGAGGGATCAACTATATGAGATCATCAAGAGCGAAGGTTTGATGACAAATACAGCTCTAAATAGAGATCCTGAAGAAAGCGTTTTGAAACCATGTACGCTTGATGAAATAAGTGAATATGTTGTGAATGCCACTGGCGCATCAGTTACAGCAGATTCCAGTGTCCGTACTATACATAAGTATTGTGACACGCTTCCCAGAGACAAGTACTTTGTTCCAAAACCAGCATTTCAATTTTCATATCTCGGAGATTCTTATGAATGTAAGATAACATTGCCACCAAATGCGGCTTTCCAAACCTTGGTTGGTCCTGCATGCAAAAACTCCGCTTTATCAAAGCAGGTTGTATGCTTAGAAGCTTGTAAGAAGCTGCATCAATTGGGTGCCTTGAATGATCATCTTCTCCCGTCCACTGATAAGCCTTTGGAGAAAGATATAAATGTAGAAAGCAAAGGGCCAGCTTCTGGTGCAGGAACAACTAAAAGGAAGGAACTACATGGGACAACTGGCATTCGCGCTCTGTCAGGAACTTGGGCAGAAAAACTTGATGGTGCCGTTTTTTACGCCTATAAATTTGACTTCTCCTGTGATATTGTTACCGAGCTATATTCTGGATTTATTCTTCTGATTGAGTCAGAGCTTGCTAAGGATGTGGGGAATATTGAATTGATGCTTTACTTGGTTTCAAAGAAAGTTAAAGCTTCTGTTTCTTCCTGTGGGCAAGTTTGTTTGGATGGAGAACAGATGGCAAAAGCAAAGCTCTTTCAGGAATTTTTCTTCAATGGATTGTTTGGGAAATTGTTTCATCGAGCCAACCCAGCTGAAAACCAGAGAGAGTTTTTACTTATGAAAGAAACAAGAAAGCTGTGGAGGCAATCATACATGTATTTGGTTCTTCCACTTGAAACATTGGATGATTCAACTAATGAATCTTGGACAATAAATTGGGGAGGGATCAGTTCTTGTGTTTCTGTGGTAGAGTTCTTGAAGCAAAATGCATTGTTGGGTGCTCAGCATTGTAAGGGTGATGCAAGGAATTCATTGCAAAGCAGAAGTGGCGCATCTGGGACTGGATGCAATAGTTCACAGATATTCCACTTTGCTGATTCTTCTGTTAATGAAAAAAATCTCAAAGATATGGTGGTAGTGGCCATCCACACCGGAAAAATATATTCTGTTTTTGAGGTTTTGAGTGACACATCCGCTGAGAGTCCTTTTGACACATTACAGTACAATACCTATGCTGAGTACTTCCACAAAAAATATGGGGTTCTGCTCATGTATCCGGGTCAGCCTCTGTTGCGGCTGAAGCAAAATCATAATCCACACAACCTTCTTGTGAACTTCAATGGCGAAGGTGGTAGTGGTAAGACATCAGAATCTGGCCCTGTTAATGGAAAGGAACGTATGTATGCCCATATGCCTCCTGAGCTTCTAGTCTGTACTGGATTGCGAAGAGACGTTTTTGAAGTCATTTTATCTACTACCATCATTGATTCATCGATTGGAATCCTTGATGTTAGCCAGCCAACTCAGAGAAGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009892 GO:0009987 GO:0010033 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0031974 GO:0031981 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0098586 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1037

Amino Acids

116.91

Weight (kDa)

6.38

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 35 - 201 5.8e-19 Type III restriction enzyme, res subunit
DEAD PF00270 39 - 201 8.3e-20 DEAD/DEAH box helicase
Helicase_C PF00271 389 - 505 1.5e-17 Helicase conserved C-terminal domain
Dicer_dimer PF03368 576 - 662 9.8e-24 Dicer dimerisation domain
PAZ PF02170 892 - 1009 1.1e-10 PAZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 753, 1541
AasI GACNNNNNNGTC 1 cut(s) 1515
Acc36I ACCTGC 1 cut(s) 1897
AccB1I GGYRCC 2 cut(s) 1949, 2117
AccB7I CCANNNNNTGG 2 cut(s) 1867, 2030
AccII CGCG 2 cut(s) 72, 2082
AciI CCGC 7 cut(s) 75, 78, 892, 1853, 1893, 2769, 2869
AclWI GGATC 4 cut(s) 916, 1581, 1628, 2528
AcoI YGGCCR 1 cut(s) 2715
AcsI RAATTY 9 cut(s) 28, 395, 1013, 1102, 1302, 1431, 2138, 2336, 2602
AcuI CTGAAG 2 cut(s) 1659, 2894
AfiI CCNNNNNNNGG 4 cut(s) 1509, 1867, 2030, 2960
AflII CTTAAG 1 cut(s) 448
AflIII ACRYGT 1 cut(s) 2448
AhlI ACTAGT 1 cut(s) 323
AjnI CCWGG 1 cut(s) 933
AjuI GAANNNNNNNTTGG 2 cut(s) 751, 783
AloI GAACNNNNNNTCC 2 cut(s) 1422, 1454
Alw21I GWGCWC 1 cut(s) 2578
Alw26I GTCTC 6 cut(s) 167, 625, 953, 1358, 1761, 3018
AlwI GGATC 4 cut(s) 916, 1581, 1628, 2528
AlwNI CAGNNNCTG 4 cut(s) 182, 2030, 2863, 2948
AoxI GGCC 6 cut(s) 22, 798, 885, 2021, 2715, 2950
ApeKI GCWGC 3 cut(s) 42, 835, 1936
ApoI RAATTY 9 cut(s) 28, 395, 1013, 1102, 1302, 1431, 2138, 2336, 2602
ArsI GACNNNNNNTTYG 2 cut(s) 1760, 1792
Asp700I GAANNNNTTC 3 cut(s) 846, 1176, 1376
AspLEI GCGC 5 cut(s) 74, 102, 1706, 2084, 2627
AspS9I GGNCC 4 cut(s) 886, 1874, 2021, 2951
AsuC2I CCSGG 1 cut(s) 2853
AsuHPI GGTGA 2 cut(s) 257, 2603
AsuII TTCGAA 2 cut(s) 95, 610
AvaII GGWCC 1 cut(s) 1874
BalI TGGCCA 1 cut(s) 2717
BanI GGYRCC 2 cut(s) 1949, 2117
BanII GRGCYC 1 cut(s) 1516
Bbv12I GWGCWC 1 cut(s) 2578
BbvI GCAGC 3 cut(s) 54, 822, 1923
BccI CCATC 9 cut(s) 163, 239, 513, 703, 2108, 2294, 2305, 2726, 3062
BceAI ACGGC 1 cut(s) 2105
BciT130I CCWGG 1 cut(s) 935
BciVI GTATCC 1 cut(s) 2859
BclI TGATCA 3 cut(s) 64, 258, 1960
BcnI CCSGG 1 cut(s) 2853
BcoDI GTCTC 6 cut(s) 167, 625, 953, 1358, 1761, 3018
BcuI ACTAGT 1 cut(s) 323
BfaI CTAG 8 cut(s) 144, 324, 564, 983, 999, 1206, 1547, 3002
BfmI CTRYAG 1 cut(s) 690
BfoI RGCGCY 1 cut(s) 103
BfrI CTTAAG 1 cut(s) 448
BfuAI ACCTGC 1 cut(s) 1897
BfuI GTATCC 1 cut(s) 2859
BisI GCNGC 7 cut(s) 43, 75, 78, 836, 1854, 1937, 2870
BlpI GCTNAGC 1 cut(s) 2577
BlsI GCNGC 7 cut(s) 44, 76, 79, 837, 1855, 1938, 2871
BmcAI AGTACT 3 cut(s) 1324, 1775, 2816
Bme1390I CCNGG 2 cut(s) 935, 2853
Bme18I GGWCC 1 cut(s) 1874
BmgT120I GGNCC 4 cut(s) 886, 1874, 2021, 2951
BmiI GGNNCC 3 cut(s) 352, 1951, 2119
BmrFI CCNGG 2 cut(s) 935, 2853
BmrI ACTGGG 1 cut(s) 1326
BmuI ACTGGG 1 cut(s) 1326
BoxI GACNNNNGTC 1 cut(s) 945
Bpu10I CCTNAGC 2 cut(s) 2211, 2994
Bpu1102I GCTNAGC 1 cut(s) 2577
Bpu14I TTCGAA 2 cut(s) 95, 610
BpuEI CTTGAG 1 cut(s) 1073
BpuMI CCSGG 1 cut(s) 2853
Bsa29I ATCGAT 1 cut(s) 3068
BsaBI GATNNNNATC 2 cut(s) 66, 3074
BsaI GGTCTC 1 cut(s) 953
BsaJI CCNNGG 3 cut(s) 354, 943, 1866
BsaWI WCCGGW 2 cut(s) 515, 2726
BsaXI ACNNNNNCTCC 2 cut(s) 583, 613
Bsc4I CCNNNNNNNGG 4 cut(s) 1509, 1867, 2030, 2960
Bse1I ACTGG 9 cut(s) 187, 420, 1321, 1498, 1705, 1725, 2077, 2641, 3016
Bse3DI GCAATG 2 cut(s) 1838, 2606
Bse8I GATNNNNATC 2 cut(s) 66, 3074
BseBI CCWGG 1 cut(s) 935
BseCI ATCGAT 1 cut(s) 3068
BseDI CCNNGG 3 cut(s) 354, 943, 1866
BseJI GATNNNNATC 2 cut(s) 66, 3074
BseLI CCNNNNNNNGG 4 cut(s) 1509, 1867, 2030, 2960
BseMI GCAATG 2 cut(s) 1838, 2606
BseMII CTCAG 6 cut(s) 294, 2591, 2763, 2802, 2985, 3113
BseNI ACTGG 9 cut(s) 187, 420, 1321, 1498, 1705, 1725, 2077, 2641, 3016
BseRI GAGGAG 2 cut(s) 353, 1439
BseXI GCAGC 3 cut(s) 54, 822, 1923
BseYI CCCAGC 1 cut(s) 2383
BsgI GTGCAG 2 cut(s) 1060, 2055
Bsh1236I CGCG 2 cut(s) 72, 2082
BshFI GGCC 6 cut(s) 24, 800, 887, 2023, 2717, 2952
BshNI GGYRCC 2 cut(s) 1949, 2117
BshVI ATCGAT 1 cut(s) 3068
BsiHKAI GWGCWC 1 cut(s) 2578
BsiSI CCGG 3 cut(s) 516, 2727, 2852
BslFI GGGAC 2 cut(s) 2080, 2647
BslI CCNNNNNNNGG 4 cut(s) 1509, 1867, 2030, 2960
BsmAI GTCTC 6 cut(s) 167, 625, 953, 1358, 1761, 3018
BsmBI CGTCTC 1 cut(s) 3018
BsmFI GGGAC 2 cut(s) 2080, 2647
BsmI GAATGC 4 cut(s) 458, 994, 1699, 2076
BsnI GGCC 6 cut(s) 24, 800, 887, 2023, 2717, 2952
Bso31I GGTCTC 1 cut(s) 953
Bsp119I TTCGAA 2 cut(s) 95, 610
Bsp1286I GDGCHC 2 cut(s) 1516, 2578
Bsp1720I GCTNAGC 1 cut(s) 2577
BspACI CCGC 7 cut(s) 75, 78, 892, 1853, 1893, 2769, 2869
BspANI GGCC 6 cut(s) 24, 800, 887, 2023, 2717, 2952
BspCNI CTCAG 6 cut(s) 295, 2590, 2764, 2803, 2986, 3112
BspDI ATCGAT 1 cut(s) 3068
BspFNI CGCG 2 cut(s) 72, 2082
BspLI GGNNCC 3 cut(s) 352, 1951, 2119
BspMI ACCTGC 1 cut(s) 1897
BspPI GGATC 4 cut(s) 916, 1581, 1628, 2528
BspT104I TTCGAA 2 cut(s) 95, 610
BspT107I GGYRCC 2 cut(s) 1949, 2117
BspTI CTTAAG 1 cut(s) 448
BspTNI GGTCTC 1 cut(s) 953
BsrDI GCAATG 2 cut(s) 1838, 2606
BsrI ACTGG 9 cut(s) 187, 420, 1321, 1498, 1705, 1725, 2077, 2641, 3016
BssECI CCNNGG 3 cut(s) 354, 943, 1866
BssT1I CCWWGG 3 cut(s) 354, 943, 1866
Bst2UI CCWGG 1 cut(s) 935
Bst4CI ACNGT 5 cut(s) 280, 638, 1218, 1483, 2796
Bst6I CTCTTC 4 cut(s) 143, 999, 3016, 3100
BstAFI CTTAAG 1 cut(s) 448
BstBI TTCGAA 2 cut(s) 95, 610
BstC8I GCNNGC 4 cut(s) 1882, 2025, 2208, 3092
BstDEI CTNAG 9 cut(s) 268, 303, 1918, 2211, 2577, 2772, 2811, 2994, 3099
BstFNI CGCG 2 cut(s) 72, 2082
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 5 cut(s) 74, 102, 1706, 2084, 2627
BstMAI GTCTC 6 cut(s) 167, 625, 953, 1358, 1761, 3018
BstNI CCWGG 1 cut(s) 935
BstNSI RCATGY 2 cut(s) 1884, 2452
BstPAI GACNNNNGTC 1 cut(s) 945
BstSCI CCNGG 2 cut(s) 933, 2851
BstSFI CTRYAG 1 cut(s) 690
BstUI CGCG 2 cut(s) 72, 2082
BstV1I GCAGC 3 cut(s) 54, 822, 1923
BstX2I RGATCY 2 cut(s) 921, 1633
BstXI CCANNNNNNTGG 1 cut(s) 1196
BstYI RGATCY 2 cut(s) 921, 1633
Bsu15I ATCGAT 1 cut(s) 3068
BsuI GTATCC 1 cut(s) 2859
BsuRI GGCC 6 cut(s) 24, 800, 887, 2023, 2717, 2952
BsuTUI ATCGAT 1 cut(s) 3068
BtgZI GCGATG 1 cut(s) 53
BtsI GCAGTG 2 cut(s) 1043, 1245
BtsIMutI CAGTG 7 cut(s) 48, 1043, 1245, 1488, 1698, 1732, 1977
BveI ACCTGC 1 cut(s) 1897
Cac8I GCNNGC 4 cut(s) 1882, 2025, 2208, 3092
CaiI CAGNNNCTG 4 cut(s) 182, 2030, 2863, 2948
CfoI GCGC 5 cut(s) 74, 102, 1706, 2084, 2627
Cfr13I GGNCC 4 cut(s) 886, 1874, 2021, 2951
ClaI ATCGAT 1 cut(s) 3068
CviAII CATG 8 cut(s) 407, 762, 1436, 1659, 1881, 2063, 2449, 2845
DdeI CTNAG 9 cut(s) 268, 303, 1918, 2211, 2577, 2772, 2811, 2994, 3099
DraI TTTAAA 2 cut(s) 331, 831
DrdI GACNNNNNNGTC 1 cut(s) 1515
DseDI GACNNNNNNGTC 1 cut(s) 1515
EaeI YGGCCR 1 cut(s) 2715
Eam1104I CTCTTC 4 cut(s) 143, 999, 3016, 3100
EarI CTCTTC 4 cut(s) 143, 999, 3016, 3100
Eco130I CCWWGG 3 cut(s) 354, 943, 1866
Eco147I AGGCCT 1 cut(s) 800
Eco24I GRGCYC 1 cut(s) 1516
Eco31I GGTCTC 1 cut(s) 953
Eco47I GGWCC 1 cut(s) 1874
Eco57I CTGAAG 2 cut(s) 1659, 2894
EcoO109I RGGNCCY 1 cut(s) 886
EcoRI GAATTC 3 cut(s) 28, 1431, 2602
EcoRII CCWGG 1 cut(s) 933
EcoT14I CCWWGG 3 cut(s) 354, 943, 1866
EcoT22I ATGCAT 2 cut(s) 6, 2566
EcoT38I GRGCYC 1 cut(s) 1516
ErhI CCWWGG 3 cut(s) 354, 943, 1866
Esp3I CGTCTC 1 cut(s) 3018
FaeI CATG 8 cut(s) 410, 765, 1439, 1662, 1884, 2066, 2452, 2848
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 2 cut(s) 2080, 2647
FatI CATG 8 cut(s) 406, 761, 1435, 1658, 1880, 2062, 2448, 2844
FauNDI CATATG 2 cut(s) 953, 2985
FbaI TGATCA 3 cut(s) 64, 258, 1960
Fnu4HI GCNGC 7 cut(s) 43, 75, 78, 836, 1854, 1937, 2870
FriOI GRGCYC 1 cut(s) 1516
Fsp4HI GCNGC 7 cut(s) 43, 75, 78, 836, 1854, 1937, 2870
FspBI CTAG 8 cut(s) 144, 324, 564, 983, 999, 1206, 1547, 3002
GlaI GCGC 5 cut(s) 73, 101, 1705, 2083, 2626
GluI GCNGC 7 cut(s) 43, 75, 78, 836, 1854, 1937, 2870
GsaI CCCAGC 1 cut(s) 2387
HaeII RGCGCY 1 cut(s) 103
HaeIII GGCC 6 cut(s) 24, 800, 887, 2023, 2717, 2952
HapII CCGG 3 cut(s) 516, 2727, 2852
HhaI GCGC 5 cut(s) 74, 102, 1706, 2084, 2627
Hin1II CATG 8 cut(s) 410, 765, 1439, 1662, 1884, 2066, 2452, 2848
Hin6I GCGC 5 cut(s) 72, 100, 1704, 2082, 2625
HinP1I GCGC 5 cut(s) 72, 100, 1704, 2082, 2625
HindIII AAGCTT 2 cut(s) 1923, 2265
HpaII CCGG 3 cut(s) 516, 2727, 2852
HphI GGTGA 2 cut(s) 257, 2603
Hpy166II GTNNAC 3 cut(s) 1977, 2651, 2908
Hpy188I TCNGA 9 cut(s) 123, 304, 655, 1344, 1814, 2193, 2203, 2944, 3102
Hpy8I GTNNAC 3 cut(s) 1977, 2651, 2908
HpyAV CCTTC 9 cut(s) 14, 108, 538, 967, 1126, 1595, 2047, 2909, 2915
HpyCH4III ACNGT 5 cut(s) 280, 638, 1218, 1483, 2796
HpyCH4IV ACGT 3 cut(s) 1462, 2971, 3027
HpyF3I CTNAG 9 cut(s) 268, 303, 1918, 2211, 2577, 2772, 2811, 2994, 3099
HpySE526I ACGT 3 cut(s) 1462, 2971, 3027
Hsp92II CATG 8 cut(s) 410, 765, 1439, 1662, 1884, 2066, 2452, 2848
HspAI GCGC 5 cut(s) 72, 100, 1704, 2082, 2625
Ksp22I TGATCA 3 cut(s) 64, 258, 1960
LmnI GCTCC 4 cut(s) 350, 379, 997, 1454
Lsp1109I GCAGC 3 cut(s) 54, 822, 1923
MaeI CTAG 8 cut(s) 144, 324, 564, 983, 999, 1206, 1547, 3002
MaeII ACGT 3 cut(s) 1462, 2971, 3027
MaeIII GTNAC 7 cut(s) 245, 1243, 1279, 1711, 1751, 2161, 2759
MfeI CAATTG 1 cut(s) 1943
MflI RGATCY 2 cut(s) 921, 1633
MhlI GDGCHC 2 cut(s) 1516, 2578
MlsI TGGCCA 1 cut(s) 2717
MluNI TGGCCA 1 cut(s) 2717
MlyI GAGTC 2 cut(s) 2207, 2785
Mox20I TGGCCA 1 cut(s) 2717
Mph1103I ATGCAT 2 cut(s) 6, 2566
MroXI GAANNNNTTC 3 cut(s) 846, 1176, 1376
MscI TGGCCA 1 cut(s) 2717
MslI CAYNNNNRTG 2 cut(s) 1229, 1541
Msp20I TGGCCA 1 cut(s) 2717
MspA1I CMGCKG 2 cut(s) 2387, 2771
MspCI CTTAAG 1 cut(s) 448
MspI CCGG 3 cut(s) 516, 2727, 2852
MspR9I CCNGG 2 cut(s) 935, 2853
MunI CAATTG 1 cut(s) 1943
Mva1269I GAATGC 4 cut(s) 458, 994, 1699, 2076
MvaI CCWGG 1 cut(s) 935
MvnI CGCG 2 cut(s) 72, 2082
NciI CCSGG 1 cut(s) 2853
NdeI CATATG 2 cut(s) 953, 2985
NlaIII CATG 8 cut(s) 410, 765, 1439, 1662, 1884, 2066, 2452, 2848
NlaIV GGNNCC 3 cut(s) 352, 1951, 2119
NmuCI GTSAC 4 cut(s) 245, 1243, 1751, 2759
NsiI ATGCAT 2 cut(s) 6, 2566
NspI RCATGY 2 cut(s) 1884, 2452
NspV TTCGAA 2 cut(s) 95, 610
PaeI GCATGC 1 cut(s) 1884
PceI AGGCCT 1 cut(s) 800
PciI ACATGT 1 cut(s) 2448
PctI GAATGC 4 cut(s) 458, 994, 1699, 2076
PdmI GAANNNNTTC 3 cut(s) 846, 1176, 1376
PflMI CCANNNNNTGG 2 cut(s) 1867, 2030
PkrI GCNGC 7 cut(s) 44, 76, 79, 837, 1855, 1938, 2871
PleI GAGTC 2 cut(s) 2206, 2784
PpsI GAGTC 2 cut(s) 2206, 2784
PscI ACATGT 1 cut(s) 2448
PshAI GACNNNNGTC 1 cut(s) 945
PsiI TTATAA 2 cut(s) 753, 1541
Psp6I CCWGG 1 cut(s) 933
PspFI CCCAGC 1 cut(s) 2383
PspGI CCWGG 1 cut(s) 933
PspN4I GGNNCC 3 cut(s) 352, 1951, 2119
PspPI GGNCC 4 cut(s) 886, 1874, 2021, 2951
PstNI CAGNNNCTG 4 cut(s) 182, 2030, 2863, 2948
PsuI RGATCY 2 cut(s) 921, 1633
PvuII CAGCTG 1 cut(s) 2387
RseI CAYNNNNRTG 2 cut(s) 1229, 1541
SatI GCNGC 7 cut(s) 43, 75, 78, 836, 1854, 1937, 2870
Sau96I GGNCC 4 cut(s) 886, 1874, 2021, 2951
ScaI AGTACT 3 cut(s) 1324, 1775, 2816
SchI GAGTC 2 cut(s) 2207, 2785
ScrFI CCNGG 2 cut(s) 935, 2853
SduI GDGCHC 2 cut(s) 1516, 2578
SfcI CTRYAG 1 cut(s) 690
SfuI TTCGAA 2 cut(s) 95, 610
SinI GGWCC 1 cut(s) 1874
SmiMI CAYNNNNRTG 2 cut(s) 1229, 1541
SmlI CTYRAG 2 cut(s) 448, 1052
SmoI CTYRAG 2 cut(s) 448, 1052
SpeI ACTAGT 1 cut(s) 323
SphI GCATGC 1 cut(s) 1884
SseBI AGGCCT 1 cut(s) 800
SsiI CCGC 7 cut(s) 75, 78, 892, 1853, 1893, 2769, 2869
SspI AATATT 1 cut(s) 2227
SspMI CTAG 8 cut(s) 144, 324, 564, 983, 999, 1206, 1547, 3002
StuI AGGCCT 1 cut(s) 800
StyD4I CCNGG 2 cut(s) 933, 2851
StyI CCWWGG 3 cut(s) 354, 943, 1866
TaaI ACNGT 5 cut(s) 280, 638, 1218, 1483, 2796
TaiI ACGT 3 cut(s) 1465, 2974, 3030
TaqI TCGA 6 cut(s) 95, 610, 770, 809, 2374, 3068
TatI WGTACW 7 cut(s) 412, 1322, 1354, 1773, 2796, 2814, 3008
TauI GCSGC 4 cut(s) 77, 80, 1856, 2872
TscAI CASTG 7 cut(s) 55, 1043, 1252, 1488, 1705, 1732, 1984
TseFI GTSAC 4 cut(s) 245, 1243, 1751, 2759
TseI GCWGC 3 cut(s) 42, 835, 1936
Tsp45I GTSAC 4 cut(s) 245, 1243, 1751, 2759
TspGWI ACGGA 1 cut(s) 1721
TspRI CASTG 7 cut(s) 55, 1043, 1252, 1488, 1705, 1732, 1984
Van91I CCANNNNNTGG 2 cut(s) 1867, 2030
Vha464I CTTAAG 1 cut(s) 448
VpaK11BI GGWCC 1 cut(s) 1874
XapI RAATTY 9 cut(s) 28, 395, 1013, 1102, 1302, 1431, 2138, 2336, 2602
XbaI TCTAGA 1 cut(s) 982
XceI RCATGY 2 cut(s) 1884, 2452
XcmI CCANNNNNNNNNTGG 2 cut(s) 1868, 2828
XmnI GAANNNNTTC 3 cut(s) 846, 1176, 1376
XspI CTAG 8 cut(s) 144, 324, 564, 983, 999, 1206, 1547, 3002
ZrmI AGTACT 3 cut(s) 1324, 1775, 2816
Zsp2I ATGCAT 2 cut(s) 6, 2566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.