MD15G1047500.v1.1

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001909
Physical Location & Seq
Reverse (-)
3257872 .. 3268466
10595 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1047500.v1.1.491

Sequence Viewer

Length: 4962 bp
ATGGAAGTAGAAGAATCCAATTCCAACCCACTCGATCATGCCGCTGATGATGATCAGTCACCGCAATCTTCCAAGAACTTCAGTCCCAGACGGTATCAGTCGGAGGTGTACGAGGTTGCGAGGCGAAGGAACACGATAGCGGTGCTGGAGACCGGTTCCGGGAAGACGATGATAGCTGTGATGCTCATCAGAGACTATGAAGGCTGCCAAACTAATAATTCAAGTGGTGGTGCCAAAAAATTGATCATCTTTTTGGCTCCAACTGTTCATCTTGTTCACCAGCAATTTAAGGTTATTAAAGAAAGCACAAATTATAACGTAGGAGAGTATTATGGTGCGTTGGGAGTTGATTCGTGGACCATGGAGCGCTGGGAAAAGAAAATGAATGAGCATGATGTGCTAGTTATGACCCCCCAGATTTTTTTGGATGCCTTAAGAAAGGCATTCTTGAGTTTGGAAGCAGTATGCTTGATGATTATTGACGAGTGCCATCGGGCTACTGGAAACCATCCTTATACAAAGATAATGAAGTTTTATCACAAGTCTGGTAATAAGCCAAAGATTTTTGGCTTGACGGCATCACCTGTTATAAAAAAAGGTGTCTCGTCCACCAAGGATTGTGAGGGCCAGTTATCAGAACTTGAAAGCATTTTAGATTCTCAGATTTATACTATTGAGGACATAACAGAGATGGAAGTATGTGTCCCTTCTGCAAAAGAAACTTGTCGATTTTATGGCCAAGCATGGTTTCTGAGTTCGGATTTGAAAGAAAAGATGGAAGCCTCTTGGTCCAAGGTTGATGCTTCATTATCAAAGTTGCAGGTATCAGTAGAAAGTAATCTGAGTGACATGGATGATAAAATTAAGACATTGCAGAAACGAATGTCTAATGACTACACAAAGATCTTGTATTGCCTTGACGAGCTTGGTCTCATATGTACTTACGAGGCTGTTAAGATCTGTTTAGAGAATGCTCCGAAATGCGCGAAGGCGTGTGAACTTTATAGAGAGGGCTCTTTGCAGTGCAGACGTTTTCTTGAGGAAGTGTTTGGTATAATTAGGGAATCGTTTCTGCATGGTTCTGAAAATTTCTTGGATTTCGGGTTTGACCATTTGAAGGCAGTAGATGTGGGCTACGTATCTCCAAAATTAAATGAACTTGTTCAAATTTTTCGTTCTTTTGGAAGATCTAGGGAAGTGCGGTGCCTCATATTTGTTGATAGAATCATCACGGCTAAAGTGATTGAAAGATTCATGAAGAAGGTTACTTCTTTGTCTCATTTTACGGTTTCCTATTTGACTGGAAGCACTACATCAGTTGACTCTCTGGCGCCAAAAATACAAAGGGAGACATTGGAGTTATTCTGCTCTGGGGAGGTCAATTTATTATTTGCTACCGATGTAGTTGAGGAGGGAATTCATGTTCCGAGCTGCTCCTGTGTGGTACGTTTTGACCTGCCAAAGACAGTTCGTAGTTACGTCCAGTCGAGGGGACGGGCTCGGCAAGATAACTCTCAGTTCATCACAATGCTGGAGAGGGGAAACAAGAAACAAAGGGATCAACTATATGAGATTGTCAGGAGTGAGTTTTTGATGACAGATACAGCTATGAAAAGAGATCCTGAAGCATGCATTTTGAAAGCATGTCCTTTGGAGCAAACAAATGTATATACTGTGGACTCCACTGGAGCTTCAGTCACTGCAGATTCTAGTGTCAGTCTTGTACATAAATATTGTGAAACGCTGCCAAGGGATAAGTACTTCACTCCAAAGCCAACTTTTCATATCTCATATCTTGGAGAGTGTTACGAGTGTAAGATCACATTGCCTCCAAATGCAGCTTTCCAAACGTTAGTTGGCCCTGTATGCAAGAAATCTTCTTTAGCAAAGCAGCTTGTCTGCTTGGAAGCGTGTCAGCAGCTACATCAAAAGGGTGCCCTGAATGACCATCTTCTCCCAGTCGTTGATGTGCCTGATGTGAAAAGAAAAGAGCAAGCTTCTGGTGCAGGAACTACTAAAAGGAAGGAACTACATGGTACAACTTGCATTCGTATGCTATCTGGAACTTGGGGGGAGAAACTTGATGCTGCCATTTTTCAGGCATATAAGTTTGACTTCTCCTGTAACATTGCCAGTGAGTTGTATTCTGGATTTGTTCTTCTCATTGAGTCGAAGCTTGCTGATGATGTGGGGAATCTCAAATTGGACCTTTACCTGATTTCGAAAACAGTTAAGGCTTCTGTTTCTTTCTGTGGGGAGGTTCATTTAGACGGGGAACAGATGGAAAAAGCAAAGTGCTTTCAGGAATTTTTCTTTAATGGATTGTTTGGGAAATTGTATGGAACCAAATCAGCTAGAAAACGTAGAGAACTTTTACTGGAGAAAGAAACAAGAAAACTGTGGACCCCATCATGCTTATATTTGCTTCTTCCAGTTGAGACATTGAATGATTCAAAAGATGAATCTTGGAGAATAAATTGGGCAGGGATCAATTCTTGTGTTTCTGTGGTAGAGTTCTTAAAGAGAAACTCGTCTTTGAGCAGTCACCAGTGTAACGTTGACACAGGGAACTCAGTTCCAAGAACTGTATCATCTGAGACTGAGTGCAACAGTGCAGGGATAGTTCACTTTGCTAATTGCTCTGTTGATGTAAAAAATCTCAAAGATACGGTGGTGGTGGCTATCCATACTGGAAGAATCTACTCTGTTGATGAGGTTATGAGTAACACTTCTGCTGAGAGTCCTTTTGATGTAAAGAAAGACAATGTATCATCACAATATACTTCATTTTCTGAGTACTTCAACAAAAAATACGGGATTGTGCTCCGGTATCCTGGTCAGCCTCTGTTGCAACTGAAACAAAGTCATAATCCGCACAACCTTCTTGTGAACTTTAATGGTGAAGGTGGTGGTGGTAAGACATTGCAATCTGGCGTTGTTATTGGAAAGCAGATTATGTATGCTCACATGCCACCTGAGCTTCTAGTTGCTATTGGTATCCCAAGAGACGTTCTAAAGTCATGTTATTTACTACCATCGTTGATTCATCGCTTGGAGTCCTTGATGCTAGCCAGTCAACTTAGAGAAGAGATAAATTATCATTCTAGCAGCTTTCGGATATCAAGCTTATTGATTCTAGAAGCACTTACAACACTAAGATGCAATGATGAATTTTCTATGGAGCGCTTGGAATTGCTCGGTGATTCAGTTTTAAAATATACTGTGAGTTGCCACCTCTTTCTCAAATATCCCAAAAAGCATGAAGGACAATTAACTAGTTGCCGCACCCTGACTATCTGTAACTCAAACCTACATAGATTGGGCATAAATCGCAAGTTACAGGGATACATCCGTGATTGTGCGTTTGACCCATGTCGTTGGGTTTCTCCAGGGCAGATTTCTCTACGACCCATTCCTTGTAATTGTGGAGTGGAGACTTTAGAAGTACCCGTAGATGGAAAATTCCAAACTGAAGACCCTAAAGTTGTGGTTGGGAAGAGCTGTGATAAGGGCCATAGATGGATGTGTTCCAAAACAATCTCTGATTGTGTTGAAGCCCTTATAGGAGCATACTATGTTGCTGGTGGATTGCCTGCTGCAATTTATATGATGAAGTGGCTTTATATTGATGTAGAACTTGAGCCTTCCTTGGTTGCTGATGCAATTACTATGGCGTCTCTTCATTCGTACACCCTGAAAGCTAATGAGATTGCAATCATGGAGTCAAAGCTTCACTATGAATTTTCTACCAAGGGTATCTTACTCGAGGCCATAACACATGCATCTGATCAAGAATCAGGAGGTGGCTACTGTTATGAGCGACTAGAATTTCTAGGTGACTCTGTGTTGGACCTGCTTATTACAAGGCATCTCTATCATAGCCACACAGATATTGATCCAGGCGAGTTAACTGACTTGCGTTCAGCTTCTGTTAATAATGAAAGTTTTGCACAAGTTGCTGTTAGACGTGATCTTCAGCAGCATCTACAGCATGCCTCTATGTTACTTCTAAGTCAGATAACAGAATATGTGAAGTCTCTTTCAGAACCTGACAGTGGTACCACGCTTCAAGGCACAAAGAGTCCTAAGGCTCTCGGAGACATGGTGGAAAGTATTGCAGGGGCAATTCTAATTGATACCAAACTTAACCTTGATGAAGTGTGGAGAATATTTGAACCATTATTATCTCCAATTGTGACTCCTGACAAACTTGAGCTGCCTCCACTACGGGAGCTGATTGAACTATGCGACTCGCTTGGGTACCATAGAAAAGAGAAGTGTACCAATAAGGGTGAGATGTTTCATGCCGAGCTTACTGTACAGCTGAAAGATGTTCTGTTGACTGGAGAGGGGTGCAATCGGACTAAAAAAGGTGCAAAAGGAGAAGCAGCTCGTCGTTTACTTAAGAAACTTGAGGAGAAAGGTTTTTCATTTTCCAAGAAGAGGAAACTTGATGCTGACCAAGTAGGCGATTCAACTTCCCGTGACATCAACCATAAGGTCGGCGACCAAGTAACTGTCAAAGATTCTTCAGGGGTAGTGGTCTATAAAAGGCGAAAGATAACTGAAATGCCTCTAACTGCACAGACAACTGGAGTTTCTCCTTCAGTAATTGACTGGAAAACCTCTAGCCCCAACAATGCCGCCCCAGTCATTGAAGCAATCAACATAAAGAAAGGAGGACCTCGATCTGCTCTTTTTCAGCTGTGCAAGAAACTGCAGTGGCCAAAGCCGGATTTTCAAATAACCGGAACTCAGTCAAGAACTCCAATCAGATTTGGTGAAAGCTCAACCAAGTACTTCAACAGTTTTGTCGCACAAGTAACCTTGCACATACCCAACTCCGGCATCATTGACTGCTCAGGAGATGCTAGACCCGATAAGGGGAGTTCAGAAGATTCTGCAGCCATCATCTTGTTTTACGAGCTTCAACGGCAGGGAAGAGTTATAATTGGCGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009892 GO:0009987 GO:0010033 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0031974 GO:0031981 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0098586 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1654

Amino Acids

184.99

Weight (kDa)

6.98

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 27 - 195 3e-16 Type III restriction enzyme, res subunit
DEAD PF00270 31 - 195 5.4e-19 DEAD/DEAH box helicase
Helicase_C PF00271 383 - 500 5.2e-16 Helicase conserved C-terminal domain
Dicer_dimer PF03368 571 - 654 6.6e-25 Dicer dimerisation domain
PAZ PF02170 885 - 1007 4.1e-13 PAZ domain
Ribonuclease_3 PF00636 1061 - 1196 1.7e-17 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1252 - 1379 1.5e-14 Ribonuclease-III-like
Ribonuclease_3 PF00636 1273 - 1380 2.2e-21 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 315, 590, 4949
Acc36I ACCTGC 3 cut(s) 811, 1464, 3861
Acc65I GGTACC 2 cut(s) 4058, 4260
AccB1I GGYRCC 6 cut(s) 230, 1203, 1330, 1934, 4058, 4260
AccII CGCG 1 cut(s) 986
AciI CCGC 8 cut(s) 42, 62, 140, 1201, 2873, 3283, 4644, 4956
AclI AACGTT 2 cut(s) 1850, 2556
AclWI GGATC 4 cut(s) 1566, 1613, 2495, 3890
AcoI YGGCCR 2 cut(s) 736, 4724
AcsI RAATTY 8 cut(s) 1087, 1167, 1416, 2306, 3170, 3461, 3740, 3827
AcuI CTGAAG 7 cut(s) 64, 1644, 1677, 3492, 3959, 4515, 4590
AcyI GRCGYC 2 cut(s) 1331, 3674
AfeI AGCGCT 2 cut(s) 368, 3185
AfiI CCNNNNNNNGG 9 cut(s) 159, 1117, 1489, 3455, 4055, 4443, 4844, 4883, 4884
AflII CTTAAG 2 cut(s) 433, 4403
AgeI ACCGGT 1 cut(s) 152
AhlI ACTAGT 1 cut(s) 3275
AjiI CACGTC 1 cut(s) 3968
AjnI CCWGG 3 cut(s) 2833, 3388, 3898
AjuI GAANNNNNNNTTGG 4 cut(s) 732, 764, 2186, 2218
AloI GAACNNNNNNTCC 2 cut(s) 1407, 1439
Alw21I GWGCWC 1 cut(s) 2826
AlwI GGATC 4 cut(s) 1566, 1613, 2495, 3890
AlwNI CAGNNNCTG 4 cut(s) 1700, 2845, 3929, 4049
Ama87I CYCGRG 1 cut(s) 3764
Aor51HI AGCGCT 2 cut(s) 368, 3185
AoxI GGCC 6 cut(s) 625, 736, 1858, 3511, 3768, 4724
ApoI RAATTY 8 cut(s) 1087, 1167, 1416, 2306, 3170, 3461, 3740, 3827
AsiGI ACCGGT 1 cut(s) 152
Asp700I GAANNNNTTC 2 cut(s) 1068, 1161
Asp718I GGTACC 2 cut(s) 4058, 4260
AspLEI GCGC 4 cut(s) 369, 986, 1333, 3186
AspS9I GGNCC 9 cut(s) 357, 625, 789, 1859, 2206, 2403, 3511, 3850, 4682
AsuC2I CCSGG 1 cut(s) 160
AsuHPI GGTGA 9 cut(s) 51, 269, 573, 2537, 2912, 3212, 3848, 4304, 4793
AsuII TTCGAA 1 cut(s) 2222
AsuNHI GCTAGC 1 cut(s) 3067
AvaI CYCGRG 1 cut(s) 3764
AvaII GGWCC 6 cut(s) 357, 789, 2206, 2403, 3850, 4682
AxyI CCTNAGG 1 cut(s) 4086
BaeGI GKGCMC 1 cut(s) 1939
BaeI ACNNNNGTAYC 6 cut(s) 2658, 2691, 3337, 3370, 4042, 4075
BalI TGGCCA 2 cut(s) 738, 4726
BanI GGYRCC 6 cut(s) 230, 1203, 1330, 1934, 4058, 4260
BanII GRGCYC 2 cut(s) 1016, 1501
BbsI GAAGAC 2 cut(s) 170, 3480
Bbv12I GWGCWC 1 cut(s) 2826
BceAI ACGGC 3 cut(s) 591, 1248, 4949
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BciT130I CCWGG 3 cut(s) 2835, 3390, 3900
BciVI GTATCC 3 cut(s) 2841, 3008, 3338
BclI TGATCA 3 cut(s) 52, 243, 3787
BcnI CCSGG 1 cut(s) 160
BcuI ACTAGT 1 cut(s) 3275
BfmI CTRYAG 4 cut(s) 1701, 3986, 4718, 4902
BfoI RGCGCY 3 cut(s) 370, 1334, 3187
BfrI CTTAAG 2 cut(s) 433, 4403
BfuAI ACCTGC 3 cut(s) 811, 1464, 3861
BfuI GTATCC 3 cut(s) 2841, 3008, 3338
BglII AGATCT 3 cut(s) 903, 957, 1187
BmcAI AGTACT 3 cut(s) 1760, 2798, 4799
Bme1390I CCNGG 4 cut(s) 160, 2835, 3390, 3900
Bme18I GGWCC 6 cut(s) 357, 789, 2206, 2403, 3850, 4682
BmeT110I CYCGRG 1 cut(s) 3764
BmgBI CACGTC 1 cut(s) 3968
BmgT120I GGNCC 9 cut(s) 357, 625, 789, 1859, 2206, 2403, 3511, 3850, 4682
BmrFI CCNGG 4 cut(s) 160, 2835, 3390, 3900
BmrI ACTGGG 2 cut(s) 1952, 4643
BmtI GCTAGC 1 cut(s) 3071
BmuI ACTGGG 2 cut(s) 1952, 4643
BoxI GACNNNNGTC 1 cut(s) 3372
BpiI GAAGAC 2 cut(s) 170, 3480
BpmI CTGGAG 7 cut(s) 167, 1553, 1707, 2399, 3372, 4365, 4614
Bpu10I CCTNAGC 2 cut(s) 2976, 4861
Bpu14I TTCGAA 1 cut(s) 2222
BpuEI CTTGAG 5 cut(s) 469, 1058, 3659, 4232, 4433
BpuMI CCSGG 1 cut(s) 160
BsaAI YACGTR 1 cut(s) 1138
BsaBI GATNNNNATC 4 cut(s) 51, 185, 3713, 3894
BsaHI GRCGYC 2 cut(s) 1331, 3674
BsaI GGTCTC 2 cut(s) 143, 935
BsaJI CCNNGG 7 cut(s) 360, 612, 792, 1748, 3389, 3648, 3750
BsaWI WCCGGW 3 cut(s) 152, 2826, 4748
BsaXI ACNNNNNCTCC 6 cut(s) 1680, 1710, 1813, 1843, 4089, 4119
Bsc4I CCNNNNNNNGG 9 cut(s) 159, 1117, 1489, 3455, 4055, 4443, 4844, 4883, 4884
Bse118I RCCGGY 1 cut(s) 152
Bse21I CCTNAGG 1 cut(s) 4086
Bse3DI GCAATG 5 cut(s) 869, 1823, 2127, 2921, 3169
Bse8I GATNNNNATC 4 cut(s) 51, 185, 3713, 3894
BseBI CCWGG 3 cut(s) 2835, 3390, 3900
BseDI CCNNGG 7 cut(s) 360, 612, 792, 1748, 3389, 3648, 3750
BseGI GGATG 5 cut(s) 433, 508, 859, 3348, 3528
BseJI GATNNNNATC 4 cut(s) 51, 185, 3713, 3894
BseLI CCNNNNNNNGG 9 cut(s) 159, 1117, 1489, 3455, 4055, 4443, 4844, 4883, 4884
BseMI GCAATG 5 cut(s) 869, 1823, 2127, 2921, 3169
BseRI GAGGAG 2 cut(s) 1424, 4430
BseSI GKGCMC 1 cut(s) 1939
BseYI CCCAGC 1 cut(s) 369
BsgI GTGCAG 4 cut(s) 1045, 2025, 2634, 4566
Bsh1236I CGCG 1 cut(s) 986
BshFI GGCC 6 cut(s) 627, 738, 1860, 3513, 3770, 4726
BshNI GGYRCC 6 cut(s) 230, 1203, 1330, 1934, 4058, 4260
BshTI ACCGGT 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 2826
BsiHKCI CYCGRG 1 cut(s) 3764
BsiSI CCGG 6 cut(s) 153, 159, 2827, 4733, 4749, 4845
BslFI GGGAC 3 cut(s) 69, 689, 1506
BslI CCNNNNNNNGG 9 cut(s) 159, 1117, 1489, 3455, 4055, 4443, 4844, 4883, 4884
BsmBI CGTCTC 2 cut(s) 3000, 3681
BsmFI GGGAC 3 cut(s) 69, 689, 1506
BsmI GAATGC 3 cut(s) 443, 976, 2046
BsnI GGCC 6 cut(s) 627, 738, 1860, 3513, 3770, 4726
Bso31I GGTCTC 2 cut(s) 143, 935
BsoBI CYCGRG 1 cut(s) 3764
Bsp119I TTCGAA 1 cut(s) 2222
Bsp1286I GDGCHC 4 cut(s) 1016, 1501, 1939, 2826
Bsp1407I TGTACA 2 cut(s) 1723, 4318
Bsp19I CCATGG 1 cut(s) 360
BspACI CCGC 8 cut(s) 42, 62, 140, 1201, 2873, 3283, 4644, 4956
BspANI GGCC 6 cut(s) 627, 738, 1860, 3513, 3770, 4726
BspFNI CGCG 1 cut(s) 986
BspHI TCATGA 1 cut(s) 1254
BspMAI CTGCAG 3 cut(s) 1705, 4722, 4906
BspMI ACCTGC 3 cut(s) 811, 1464, 3861
BspOI GCTAGC 1 cut(s) 3071
BspPI GGATC 4 cut(s) 1566, 1613, 2495, 3890
BspQI GCTCTTC 1 cut(s) 3491
BspT104I TTCGAA 1 cut(s) 2222
BspT107I GGYRCC 6 cut(s) 230, 1203, 1330, 1934, 4058, 4260
BspTI CTTAAG 2 cut(s) 433, 4403
BspTNI GGTCTC 2 cut(s) 143, 935
BsrDI GCAATG 5 cut(s) 869, 1823, 2127, 2921, 3169
BsrFI RCCGGY 1 cut(s) 152
BsrGI TGTACA 2 cut(s) 1723, 4318
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 7 cut(s) 360, 612, 792, 1748, 3389, 3648, 3750
BssNI GRCGYC 2 cut(s) 1331, 3674
BssT1I CCWWGG 6 cut(s) 360, 612, 792, 1748, 3648, 3750
Bst2UI CCWGG 3 cut(s) 2835, 3390, 3900
Bst6I CTCTTC 5 cut(s) 3081, 3491, 3684, 4436, 4936
BstACI GRCGYC 2 cut(s) 1331, 3674
BstAFI CTTAAG 2 cut(s) 433, 4403
BstAPI GCANNNNNTGC 2 cut(s) 397, 3956
BstAUI TGTACA 2 cut(s) 1723, 4318
BstBAI YACGTR 1 cut(s) 1138
BstBI TTCGAA 1 cut(s) 2222
BstC8I GCNNGC 6 cut(s) 1630, 1995, 2178, 3069, 3594, 3993
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 5 cut(s) 433, 508, 859, 3348, 3528
BstFNI CGCG 1 cut(s) 986
BstH2I RGCGCY 3 cut(s) 370, 1334, 3187
BstHHI GCGC 4 cut(s) 369, 986, 1333, 3186
BstMWI GCNNNNNNNGC 9 cut(s) 397, 1866, 2003, 2848, 2977, 3330, 3956, 3988, 4933
BstNI CCWGG 3 cut(s) 2835, 3390, 3900
BstNSI RCATGY 5 cut(s) 1632, 1647, 2971, 3782, 3995
BstPAI GACNNNNGTC 1 cut(s) 3372
BstSCI CCNGG 4 cut(s) 158, 2833, 3388, 3898
BstSFI CTRYAG 4 cut(s) 1701, 3986, 4718, 4902
BstSLI GKGCMC 1 cut(s) 1939
BstSNI TACGTA 1 cut(s) 1138
BstUI CGCG 1 cut(s) 986
BstV2I GAAGAC 2 cut(s) 170, 3480
BstX2I RGATCY 4 cut(s) 903, 957, 1187, 1618
BstXI CCANNNNNNTGG 1 cut(s) 3378
BstYI RGATCY 4 cut(s) 903, 957, 1187, 1618
Bsu36I CCTNAGG 1 cut(s) 4086
BsuI GTATCC 3 cut(s) 2841, 3008, 3338
BsuRI GGCC 6 cut(s) 627, 738, 1860, 3513, 3770, 4726
BtgI CCRYGG 1 cut(s) 360
BtgZI GCGATG 1 cut(s) 3032
BtrI CACGTC 1 cut(s) 3968
BtsCI GGATG 5 cut(s) 433, 508, 859, 3348, 3528
BtsI GCAGTG 3 cut(s) 1028, 1698, 4727
BtsIMutI CAGTG 8 cut(s) 1028, 1683, 1698, 2140, 2555, 2617, 4060, 4727
BveI ACCTGC 3 cut(s) 811, 1464, 3861
Cac8I GCNNGC 6 cut(s) 1630, 1995, 2178, 3069, 3594, 3993
CaiI CAGNNNCTG 4 cut(s) 1700, 2845, 3929, 4049
CciI TCATGA 1 cut(s) 1254
CfoI GCGC 4 cut(s) 369, 986, 1333, 3186
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 9 cut(s) 357, 625, 789, 1859, 2206, 2403, 3511, 3850, 4682
CseI GACGC 1 cut(s) 3663
CspAI ACCGGT 1 cut(s) 152
DinI GGCGCC 1 cut(s) 1332
DraI TTTAAA 1 cut(s) 3213
EaeI YGGCCR 2 cut(s) 736, 4724
Eam1104I CTCTTC 5 cut(s) 3081, 3491, 3684, 4436, 4936
EarI CTCTTC 5 cut(s) 3081, 3491, 3684, 4436, 4936
Eco105I TACGTA 1 cut(s) 1138
Eco130I CCWWGG 6 cut(s) 360, 612, 792, 1748, 3648, 3750
Eco24I GRGCYC 2 cut(s) 1016, 1501
Eco31I GGTCTC 2 cut(s) 143, 935
Eco32I GATATC 1 cut(s) 3120
Eco47I GGWCC 6 cut(s) 357, 789, 2206, 2403, 3850, 4682
Eco47III AGCGCT 2 cut(s) 368, 3185
Eco57I CTGAAG 7 cut(s) 64, 1644, 1677, 3492, 3959, 4515, 4590
Eco81I CCTNAGG 1 cut(s) 4086
Eco88I CYCGRG 1 cut(s) 3764
EcoO109I RGGNCCY 1 cut(s) 4682
EcoRI GAATTC 1 cut(s) 1416
EcoRII CCWGG 3 cut(s) 2833, 3388, 3898
EcoRV GATATC 1 cut(s) 3120
EcoT14I CCWWGG 6 cut(s) 360, 612, 792, 1748, 3648, 3750
EcoT22I ATGCAT 2 cut(s) 1634, 3784
EcoT38I GRGCYC 2 cut(s) 1016, 1501
EgeI GGCGCC 1 cut(s) 1332
EheI GGCGCC 1 cut(s) 1332
ErhI CCWWGG 6 cut(s) 360, 612, 792, 1748, 3648, 3750
Esp3I CGTCTC 2 cut(s) 3000, 3681
FalI AAGNNNNNCTT 6 cut(s) 431, 463, 2099, 2131, 3743, 3775
FaqI GGGAC 3 cut(s) 69, 689, 1506
FauI CCCGC 1 cut(s) 4949
FauNDI CATATG 1 cut(s) 935
FbaI TGATCA 3 cut(s) 52, 243, 3787
FokI GGATG 5 cut(s) 440, 495, 866, 3335, 3535
FriOI GRGCYC 2 cut(s) 1016, 1501
GlaI GCGC 4 cut(s) 368, 985, 1332, 3185
GsaI CCCAGC 1 cut(s) 373
GsuI CTGGAG 7 cut(s) 167, 1553, 1707, 2399, 3372, 4365, 4614
HaeII RGCGCY 3 cut(s) 370, 1334, 3187
HaeIII GGCC 6 cut(s) 627, 738, 1860, 3513, 3770, 4726
HapII CCGG 6 cut(s) 153, 159, 2827, 4733, 4749, 4845
HgaI GACGC 1 cut(s) 3663
HhaI GCGC 4 cut(s) 369, 986, 1333, 3186
Hin1I GRCGYC 2 cut(s) 1331, 3674
Hin6I GCGC 4 cut(s) 367, 984, 1331, 3184
HinP1I GCGC 4 cut(s) 367, 984, 1331, 3184
HincII GTYRAC 5 cut(s) 1321, 2560, 3077, 3909, 4341
HindII GTYRAC 5 cut(s) 1321, 2560, 3077, 3909, 4341
HindIII AAGCTT 4 cut(s) 1995, 2174, 3124, 3728
HpaI GTTAAC 1 cut(s) 3909
HpaII CCGG 6 cut(s) 153, 159, 2827, 4733, 4749, 4845
HphI GGTGA 9 cut(s) 51, 269, 573, 2537, 2912, 3212, 3848, 4304, 4793
Hpy99I CGWCG 1 cut(s) 4398
HpyF10VI GCNNNNNNNGC 9 cut(s) 397, 1866, 2003, 2848, 2977, 3330, 3956, 3988, 4933
Hsp92I GRCGYC 2 cut(s) 1331, 3674
HspAI GCGC 4 cut(s) 367, 984, 1331, 3184
KasI GGCGCC 1 cut(s) 1330
KpnI GGTACC 2 cut(s) 4062, 4264
Ksp22I TGATCA 3 cut(s) 52, 243, 3787
KspAI GTTAAC 1 cut(s) 3909
LguI GCTCTTC 1 cut(s) 3491
MfeI CAATTG 1 cut(s) 4191
MflI RGATCY 4 cut(s) 903, 957, 1187, 1618
MhlI GDGCHC 4 cut(s) 1016, 1501, 1939, 2826
MlsI TGGCCA 2 cut(s) 738, 4726
MluNI TGGCCA 2 cut(s) 738, 4726
Mly113I GGCGCC 1 cut(s) 1331
MmeI TCCRAC 4 cut(s) 48, 81, 284, 3828
Mox20I TGGCCA 2 cut(s) 738, 4726
Mph1103I ATGCAT 2 cut(s) 1634, 3784
MroXI GAANNNNTTC 2 cut(s) 1068, 1161
MscI TGGCCA 2 cut(s) 738, 4726
MslI CAYNNNNRTG 3 cut(s) 1526, 2051, 3157
Msp20I TGGCCA 2 cut(s) 738, 4726
MspA1I CMGCKG 3 cut(s) 44, 4324, 4705
MspCI CTTAAG 2 cut(s) 433, 4403
MspI CCGG 6 cut(s) 153, 159, 2827, 4733, 4749, 4845
MspR9I CCNGG 4 cut(s) 160, 2835, 3390, 3900
MunI CAATTG 1 cut(s) 4191
Mva1269I GAATGC 3 cut(s) 443, 976, 2046
MvaI CCWGG 3 cut(s) 2835, 3390, 3900
MvnI CGCG 1 cut(s) 986
MwoI GCNNNNNNNGC 9 cut(s) 397, 1866, 2003, 2848, 2977, 3330, 3956, 3988, 4933
NarI GGCGCC 1 cut(s) 1331
NciI CCSGG 1 cut(s) 160
NcoI CCATGG 1 cut(s) 360
NdeI CATATG 1 cut(s) 935
NheI GCTAGC 1 cut(s) 3067
NmeAIII GCCGAG 2 cut(s) 1480, 4333
NmuCI GTSAC 7 cut(s) 57, 845, 1696, 2543, 3836, 4195, 4484
NsiI ATGCAT 2 cut(s) 1634, 3784
NspI RCATGY 5 cut(s) 1632, 1647, 2971, 3782, 3995
NspV TTCGAA 1 cut(s) 2222
PaeI GCATGC 2 cut(s) 1632, 3995
PaeR7I CTCGAG 1 cut(s) 3764
PagI TCATGA 1 cut(s) 1254
PciSI GCTCTTC 1 cut(s) 3491
PctI GAATGC 3 cut(s) 443, 976, 2046
PdmI GAANNNNTTC 2 cut(s) 1068, 1161
PfoI TCCNGGA 1 cut(s) 158
PinAI ACCGGT 1 cut(s) 152
PluTI GGCGCC 1 cut(s) 1334
Ppu21I YACGTR 1 cut(s) 1138
PpuMI RGGWCCY 1 cut(s) 4682
PshAI GACNNNNGTC 1 cut(s) 3372
PsiI TTATAA 3 cut(s) 315, 590, 4949
Psp1406I AACGTT 2 cut(s) 1850, 2556
Psp5II RGGWCCY 1 cut(s) 4682
Psp6I CCWGG 3 cut(s) 2833, 3388, 3898
PspFI CCCAGC 1 cut(s) 369
PspGI CCWGG 3 cut(s) 2833, 3388, 3898
PspPI GGNCC 9 cut(s) 357, 625, 789, 1859, 2206, 2403, 3511, 3850, 4682
PspPPI RGGWCCY 1 cut(s) 4682
PspXI VCTCGAGB 1 cut(s) 3764
PstI CTGCAG 3 cut(s) 1705, 4722, 4906
PstNI CAGNNNCTG 4 cut(s) 1700, 2845, 3929, 4049
PsuI RGATCY 4 cut(s) 903, 957, 1187, 1618
PvuII CAGCTG 2 cut(s) 4324, 4705
RseI CAYNNNNRTG 3 cut(s) 1526, 2051, 3157
SapI GCTCTTC 1 cut(s) 3491
Sau96I GGNCC 9 cut(s) 357, 625, 789, 1859, 2206, 2403, 3511, 3850, 4682
ScaI AGTACT 3 cut(s) 1760, 2798, 4799
ScrFI CCNGG 4 cut(s) 160, 2835, 3390, 3900
SduI GDGCHC 4 cut(s) 1016, 1501, 1939, 2826
SfcI CTRYAG 4 cut(s) 1701, 3986, 4718, 4902
SfoI GGCGCC 1 cut(s) 1332
Sfr274I CTCGAG 1 cut(s) 3764
SfuI TTCGAA 1 cut(s) 2222
SinI GGWCC 6 cut(s) 357, 789, 2206, 2403, 3850, 4682
SlaI CTCGAG 1 cut(s) 3764
SmiMI CAYNNNNRTG 3 cut(s) 1526, 2051, 3157
SmlI CTYRAG 8 cut(s) 433, 448, 1037, 3638, 3764, 4211, 4403, 4412
SmoI CTYRAG 8 cut(s) 433, 448, 1037, 3638, 3764, 4211, 4403, 4412
SnaBI TACGTA 1 cut(s) 1138
SpeI ACTAGT 1 cut(s) 3275
SphI GCATGC 2 cut(s) 1632, 3995
SsiI CCGC 8 cut(s) 42, 62, 140, 1201, 2873, 3283, 4644, 4956
SspDI GGCGCC 1 cut(s) 1330
SspI AATATT 2 cut(s) 1733, 4170
StyD4I CCNGG 4 cut(s) 158, 2833, 3388, 3898
StyI CCWWGG 6 cut(s) 360, 612, 792, 1748, 3648, 3750
TaqI TCGA 7 cut(s) 33, 727, 1487, 2171, 2222, 3765, 4687
TatI WGTACW 6 cut(s) 938, 1723, 1758, 2796, 4318, 4797
TauI GCSGC 3 cut(s) 44, 3285, 4646
TscAI CASTG 8 cut(s) 1028, 1690, 1705, 2140, 2555, 2617, 4060, 4727
TseFI GTSAC 7 cut(s) 57, 845, 1696, 2543, 3836, 4195, 4484
Tsp45I GTSAC 7 cut(s) 57, 845, 1696, 2543, 3836, 4195, 4484
TspGWI ACGGA 1 cut(s) 3341
TspRI CASTG 8 cut(s) 1028, 1690, 1705, 2140, 2555, 2617, 4060, 4727
Vha464I CTTAAG 2 cut(s) 433, 4403
VpaK11BI GGWCC 6 cut(s) 357, 789, 2206, 2403, 3850, 4682
XapI RAATTY 8 cut(s) 1087, 1167, 1416, 2306, 3170, 3461, 3740, 3827
XbaI TCTAGA 1 cut(s) 3136
XceI RCATGY 5 cut(s) 1632, 1647, 2971, 3782, 3995
XcmI CCANNNNNNNNNTGG 2 cut(s) 497, 1853
XhoI CTCGAG 1 cut(s) 3764
XmnI GAANNNNTTC 2 cut(s) 1068, 1161
ZrmI AGTACT 3 cut(s) 1760, 2798, 4799
Zsp2I ATGCAT 2 cut(s) 1634, 3784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.