Rh6DG512000

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001909
Physical Location & Seq
Forward (+)
66492660 .. 66502370
9711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG512000.1

Sequence Viewer

Length: 4878 bp
ATGCATTTGCCAGAGGAAGAAGGCCAGATTCCCCTGAAACGCAGCTTCACTGAAACCACTGATGATCATGGTGCCGCCGCCGATCAAGGAGTTTCAAAGCGCCTCAATCCCAGAAGGTATCAGACACAAGTGTATGAGGTTGCTAGAAAGAGGAACACAATAGCTGTGATGGAGACAGGTACTGGCAAGACAATGATAGCTGTGATGCTCATTGATGAAATTGGTCAAGCTATCAAGTCATCTGGTGACAACAAATTGATCATATTCTTGGCCCCAACTGTTCATCTTGTTCATCAGCAATTTGAGGTTATTAAAGAACACACTACTTTTAAAGTAGAGGAGTATTATGGAGCCAAGGGGGTTGATGCGTGGGATAAGGAGCATTGGGAAAAGGAATTTAAAACCCATGATGTACTGGTTATGACACCCCAGATTCTTTTGGATGCCTTAAGAAAGGCATTCTTGACCATAGAAGCAGTATGCTTGGTGATTATGGATGAGTGCCATCGGGCTACCGGAAACCACCCTTATACAAAGATAATGAAGGAGTTTTATCACAGGTCTAGTATCAAGCCAAAAGTTTTTGGAATGACGGCTTCTCCTGTAATTCGAAAGGGTGTCTCATCCACAATGGACTGTGAAGATCAAATATCTGAACTTGAAAGCATTTTGGATTCCCAGATTTATACTATAGAGGATAAAACAGAGATGGAAGTATATGCCCCTACTGCAAAAGAAACTCGTAGATTTTATAACCCAACATGGTTTTCGAGTTTGGATTTGAAAGAAAAGATCAAGGCCTCTTGGTCGAAGAATGATGCTTCGTTTTTAAAGCTGCAAGAATCAGTTCAAAGCAATTTCAAAGATTTGGATGACAAAGTTAAGGCCCTGCGGAAACGATTGTCTAATGACTATGAAAAGATCCTGTATTGCCTGGACGGCCTTGGTCTCATATGTGCTTATGAGGCTGTGAAGGTCTGTCTAGAGAATGCTCCTACTAGCGAAGAGTGTGAATTTTATAGAGAAAGTTCTTTGCAGTGCAGATATTTTCTTGAGGAAGTTTTTGGTATAATCAAGCAATCTCTTTCGAATGGTAGTGAAAATTTTTTGGATGTTGGGTTTGACTATTTGAAGGCAGTAGATTTGGGCTACATATCTCCAAAGTTAAATGAACTTGTTCAGCTTTTCCATTCGTTTGGAGTATCTAGGGAACTACTGTGCCTCATCTTTGTGGATAGAATTGTCACTGCTAAAGTGATTGAAAGATTTATGAGAAAAGTTACCTCCTTATCTCATTTCAAAATTTCCTATTTGACTGGGAGTACTACATCAGTTGATGCTCTGACACCAAAAGTACAAAAGGAGACATTGAAGTTATTCAGCTCTGGGGAGGTCAATTTATTATTTTCTACCGATGTAGTTGAGGAGGGAATTCATGTTCCAAACTGCTCCTGTGTGATACGTTTTGACTTGCCAAAGACAGTGCGTAGTTATGTCCAGTCCAGAGGACGGGCTCGTCAAAATAACTCTCAATTCATTATAATGCTAGAAAGGGGAAACAAGCAACAGAGGGATCAACTATATGAGATCATCAAGAGCGAAGGTTTGATGACAAATACAGCTCTAAATAGAGATCCTGAAGAAAGCGTTTTGAAACCATGTACACTTGATGAAATAAGTGAATATGTTGTGAATGCCACTGGAGCATCAGTTACAGCAGATTCCAGTGTCCGTACTATACATAAGTATTGTGACACGCTTCCCAGAGACAAGTACTTTGTTCCAAGACCAGCATTTCAATTTTCACATCTCGGAGATTCTTATGAATGTAAGATTACATTGCCTCCAAATGCGGCTTTCCAAACCTTGGTCGGTCCTGCATGCAAAAACTCCGCTTTATCAAAGCAGGTTGTATGCTTAGAAGCTTGTAAGAAGCTGCATCAATTGGGTGCCTTGAATGATCATCTTCTCCCATCCACTGATAAGCCTTTGGAGAAAGATATAAATGTAGAAAGCAAAGGGCCAGCTTCTGGTGCAGGAACAACTAAAAGGAAGGAACTACATGGGACAACTGGCATTCGCGCTCTGTCAGGAACTTGGGCAGAAAAACTTGATGGTGCCGTTTTTTACGCTTATAAATTTGACTTCTCCTGTGATATTGTTACCGAGCTATATTCTGGATTTATTCTTCTGATTGAGTCCGAGCTTGCTAAGGATGTGGGGAATATTGAATTGACGCTTTACTTGGTTTCAAAGAAAGTTAAAGCTTCTGTTTCTTCCTGTGGGCAAGTTCGTTTGGATGGAGAACAGATGGCAAAAGCAAAGCTCTTTCAGGAATTTTTCTTCAATGGATTGTTTGGGAAATTGTTTCATCGAGCCAACCCAGCTGAAAAGCAGAGAGAGTTTCTACTTATGAAAGAAACAAGAAAGCTGTGGAGGCAATCATACATGTATTTGGTTCTTCCGCTTGAAACATTGGATGATTCAAATAATGAATCTTGGACAATAAATTGGGGAGGGATCAGTTCTTGTGTTTCTGTGGTAGAGTTCTTGAAGCAAAATGCATTGTTGGGTGCTCAGCATTGTAAGGGTGACACAAGGAATTCATTGCAAAGCAGAAGTGGCGCATCTGGGACTGGATGCAATAGTTCACAGATATTCCACTTTGCTGATTCTTCTGTTAATGAAAAAAATCTCAAAGATATGGTGGTAGTGGCCATCCACACTGGAAAAATATATTCTGTTTTTGAGGTTTTGAGTGACACATCCGCTGAGAGTCCTTTTGACACATCACAGTACAATACCTATGCTGAGTACTTCCACAAAAAATATGGGGTTCTGCTCATGTATCCGGGTCAGCCTTTGTTGCGGCTGAAGCAAAATCATAATCCACACAACCTTCTTGTGAACTTCAATGGCGAAGGTGGTAGTGGTAAGACATCAGAATCTGGCCTTGTTAATGGAAAGGAACGTATGTATGCCCATATGCCTCCTGAGCTTCTAGTCTGTACTGGATTGCGAAGAGACGTTTTGAAGTCATTTTATCTACTACCATCATTGATTCATCGATTGGAATCCTTGATGTTAGCCAGCCAACTCAGAGAAGAGATTGACAGTCATTGCAGCTTACAGATATCAAGCTCCTTGATTCTAGAAGCACTTACATCACTCAGATGCAGTGAAGATTTTTCCATGGAACGTTTGGAATTGCTTGGTGATTCAGTGCTAAAGTATACTGTAGGCTGCCACCTCTTCCTAAAATATCCCGAAAAACATGATGGACAATTATCGTCTCTGCGCCAGTCTGTAATTTGTAACGCTAACCTGCATAAATTGGGAATAGATCGAAACTTACAGGGATATATACGTGATGGTGCTTTTGATCCACGCCGTTGGGTTGGTCCAGGACAGGTCTCTATACGACCTGTTCCTTGTGAGTGTGGAGTGGATACCTTAGAAGTTCCAGTAGATAGCAAGTTTCAAACTAGAGACCGCGAAGTTGTGGTTGGAAAGTGCTGTGACAAAGGCCATAGATGGATGGGTTCCAAAACAATCTCTGATTGCGTTGAAGCCCTTATAGGAGCTTACTATGTAGCTGGTGGATTATCTGCTGCAGTCCATTTTATGAAGTGGCTCCAAATTGATGTAGAACTTGAGCCATCATTGGTTTCTGAAGCAATTACTACTGCATCACTTCATTCCTGCAACCCAAAAGTGGATGAGATTGGAATCCTAGAGTCAAAGCTTCGTTATGTATTTTCTACCAAGGGTCTCTTACAGGAAGCTATAACACATGCATCTGAGCAAGAATCAAAACTTGGCTATTCTTATGAGAGACTAGAGTTTCTCGGTGATTGTGTGTTGGACCTGCTTATCACATGGTATCTCTATCAGAGCCACAAAGATATTGATCCAGGCGAGTTAACTGACTTGCGTGCCGCTTCAGTTAACAATGAAAACTTTGCTCAAGCTGCTGTGAGACACAACCTTCAACAGCATCTACAACATTGCTCTGGGTTACTTCAAATTCAAATAACAGAATATGTAAAGTTGCTTTCTGAACATGATAGTGGTGACAAACTTCAAGGGTCAAAAGGTCCTAAGGCTCTTGGAGACATGGTGGAAAGTATTGCAGGGGCAATACTAATTGATACGGAGCTCAACCTTGACGAAGTGTGGAGAGTATACAAACCACTGTTATCTCCAATTGTGACCCCTGATAAACTTGAGCTGCCTCCCTTGCGTGAACTGATTGAATTATGTGGATCACTTGGGTACTTTTATAAAGAACAAACTGTAAAGAAGGGTGAAACAGTTCACGCGGAGCTTAGTGTACAGCTGAAAGATGTCCTGTTGATTGGAGAGGGGATTGATCGGAGTAAGAAAATCGCAAAAGGAGAAGCAGCTCATCATTTATTGAAGGAGCTTGAGAAAAGAGGTATTACACACTCTGGGTCTGCCAGGAAGAGAAAACTGGGTGCTGACCAAGTTGATGATTCATCTCCAATAAAGCAAAAGAAAACTGAGATGCATCTGCCTATGGATGCAACTGGACTTTCTTTGCCAGCCACTGACAGAAAAATAAGTGGTTCGAAAAATGACATCCCAGTTATCAAAGCAATCAACATGAAGAAAGGAGGACCTCGAGTCTCTCTTTACGAGCTGTGCAAACAACTACAATGGCAAATGCCTACCTTCAAACCAACAGAAAAGGATTCCAGTTTCATATCAACAATAACGTTGTACATACCCAATTTGGGCAACATCGAATGCACAGGAGATCCTAGGTCAGATAAGAAGAGTTCACTAGACTCTGCAGCACTTCTCATGCTTTATGATCTCGAAAGGCAGGGTAAAATTATCATTGGTGGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009892 GO:0009987 GO:0010033 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0031974 GO:0031981 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0098586 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1625

Amino Acids

182.22

Weight (kDa)

6.39

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 35 - 201 3.1e-18 Type III restriction enzyme, res subunit
DEAD PF00270 39 - 201 2.8e-20 DEAD/DEAH box helicase
Helicase_C PF00271 389 - 505 2.2e-17 Helicase conserved C-terminal domain
Dicer_dimer PF03368 576 - 662 9.5e-24 Dicer dimerisation domain
PAZ PF02170 892 - 1019 2.3e-11 PAZ domain
Ribonucleas_3_3 PF14622 1064 - 1123 4.7e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 1066 - 1201 3.1e-17 Ribonuclease III domain
Ribonucleas_3_3 PF14622 1257 - 1384 4e-15 Ribonuclease-III-like
Ribonuclease_3 PF00636 1278 - 1385 2.5e-19 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 753, 1541, 2136, 4284
AasI GACNNNNNNGTC 1 cut(s) 1515
Acc36I ACCTGC 3 cut(s) 1897, 3333, 3876
AccB1I GGYRCC 3 cut(s) 71, 1949, 2117
AccB7I CCANNNNNTGG 2 cut(s) 1867, 2030
AccI GTMKAC 2 cut(s) 3233, 4185
AccII CGCG 3 cut(s) 2082, 3495, 4322
AclI AACGTT 2 cut(s) 3199, 4739
AclWI GGATC 8 cut(s) 916, 1581, 1628, 2528, 3377, 3905, 4273, 4775
AcoI YGGCCR 1 cut(s) 2715
AcsI RAATTY 9 cut(s) 395, 1013, 1102, 1302, 1431, 2138, 2336, 2602, 4026
AcuI CTGAAG 4 cut(s) 1659, 2894, 3693, 3927
AfiI CCNNNNNNNGG 5 cut(s) 1509, 1867, 2030, 3715, 4757
AflII CTTAAG 1 cut(s) 448
AflIII ACRYGT 1 cut(s) 2448
AhdI GACNNNNNGTC 1 cut(s) 4646
AjnI CCWGG 4 cut(s) 933, 3403, 3913, 4460
AjuI GAANNNNNNNTTGG 6 cut(s) 751, 783, 3489, 3521, 3801, 3833
AloI GAACNNNNNNTCC 2 cut(s) 1422, 1454
Alw21I GWGCWC 2 cut(s) 2578, 4161
AlwI GGATC 8 cut(s) 916, 1581, 1628, 2528, 3377, 3905, 4273, 4775
AlwNI CAGNNNCTG 4 cut(s) 182, 2030, 2948, 4571
Ama87I CYCGRG 1 cut(s) 4644
AoxI GGCC 9 cut(s) 22, 270, 798, 885, 940, 2021, 2715, 2950, 3526
ApoI RAATTY 9 cut(s) 395, 1013, 1102, 1302, 1431, 2138, 2336, 2602, 4026
ArsI GACNNNNNNTTYG 2 cut(s) 1760, 1792
Asp700I GAANNNNTTC 4 cut(s) 846, 1176, 1376, 4314
AspA2I CCTAGG 1 cut(s) 4784
AspLEI GCGC 4 cut(s) 102, 2084, 2627, 3300
AspS9I GGNCC 8 cut(s) 271, 886, 1874, 2021, 3401, 3865, 4097, 4640
AsuC2I CCSGG 1 cut(s) 2853
AsuHPI GGTGA 7 cut(s) 257, 499, 2603, 3227, 3863, 4085, 4319
AsuII TTCGAA 3 cut(s) 610, 1088, 4592
AvaI CYCGRG 1 cut(s) 4644
AvaII GGWCC 5 cut(s) 1874, 3401, 3865, 4097, 4640
AvrII CCTAGG 1 cut(s) 4784
AxyI CCTNAGG 1 cut(s) 4101
BalI TGGCCA 1 cut(s) 2717
BanI GGYRCC 3 cut(s) 71, 1949, 2117
BanII GRGCYC 2 cut(s) 1516, 4161
Bbv12I GWGCWC 2 cut(s) 2578, 4161
BceAI ACGGC 4 cut(s) 609, 955, 2105, 3375
BciT130I CCWGG 4 cut(s) 935, 3405, 3915, 4462
BciVI GTATCC 2 cut(s) 2859, 3442
BclI TGATCA 3 cut(s) 64, 258, 1960
BcnI CCSGG 1 cut(s) 2853
BfmI CTRYAG 4 cut(s) 690, 3237, 3612, 4815
BfoI RGCGCY 1 cut(s) 103
BfrI CTTAAG 1 cut(s) 448
BfuAI ACCTGC 3 cut(s) 1897, 3333, 3876
BfuI GTATCC 2 cut(s) 2859, 3442
BglI GCCNNNNNGGC 1 cut(s) 939
BlnI CCTAGG 1 cut(s) 4784
BlpI GCTNAGC 1 cut(s) 2577
BmcAI AGTACT 3 cut(s) 1324, 1775, 2816
Bme1390I CCNGG 5 cut(s) 935, 2853, 3405, 3915, 4462
Bme18I GGWCC 5 cut(s) 1874, 3401, 3865, 4097, 4640
BmeRI GACNNNNNGTC 1 cut(s) 4646
BmeT110I CYCGRG 1 cut(s) 4644
BmgT120I GGNCC 8 cut(s) 271, 886, 1874, 2021, 3401, 3865, 4097, 4640
BmiI GGNNCC 7 cut(s) 73, 273, 352, 1951, 2119, 3544, 3635
BmrFI CCNGG 5 cut(s) 935, 2853, 3405, 3915, 4462
BmrI ACTGGG 3 cut(s) 1326, 4484, 4601
BmuI ACTGGG 3 cut(s) 1326, 4484, 4601
BpmI CTGGAG 1 cut(s) 1722
Bpu10I CCTNAGC 2 cut(s) 2211, 2994
Bpu1102I GCTNAGC 1 cut(s) 2577
Bpu14I TTCGAA 3 cut(s) 610, 1088, 4592
BpuEI CTTGAG 5 cut(s) 1073, 3674, 3951, 4247, 4448
BpuMI CCSGG 1 cut(s) 2853
Bsa29I ATCGAT 1 cut(s) 3067
BsaAI YACGTR 1 cut(s) 3368
BsaBI GATNNNNATC 3 cut(s) 3073, 3728, 3909
BsaI GGTCTC 4 cut(s) 953, 3418, 3483, 3776
BsaJI CCNNGG 6 cut(s) 354, 943, 1866, 3192, 3765, 4784
BsaWI WCCGGW 1 cut(s) 515
BsaXI ACNNNNNCTCC 8 cut(s) 583, 613, 1695, 1725, 1828, 1858, 4104, 4134
Bsc4I CCNNNNNNNGG 5 cut(s) 1509, 1867, 2030, 3715, 4757
Bse21I CCTNAGG 1 cut(s) 4101
Bse3DI GCAATG 4 cut(s) 1838, 2606, 3118, 4006
Bse8I GATNNNNATC 3 cut(s) 3073, 3728, 3909
BseBI CCWGG 4 cut(s) 935, 3405, 3915, 4462
BseCI ATCGAT 1 cut(s) 3067
BseDI CCNNGG 6 cut(s) 354, 943, 1866, 3192, 3765, 4784
BseJI GATNNNNATC 3 cut(s) 3073, 3728, 3909
BseLI CCNNNNNNNGG 5 cut(s) 1509, 1867, 2030, 3715, 4757
BseMI GCAATG 4 cut(s) 1838, 2606, 3118, 4006
BseMII CTCAG 8 cut(s) 2591, 2763, 2802, 2985, 3112, 3184, 3792, 4515
BseRI GAGGAG 2 cut(s) 353, 1439
BseYI CCCAGC 1 cut(s) 2383
BsgI GTGCAG 2 cut(s) 1060, 2055
Bsh1236I CGCG 3 cut(s) 2082, 3495, 4322
BshFI GGCC 9 cut(s) 24, 272, 800, 887, 942, 2023, 2717, 2952, 3528
BshNI GGYRCC 3 cut(s) 71, 1949, 2117
BshVI ATCGAT 1 cut(s) 3067
BsiHKAI GWGCWC 2 cut(s) 2578, 4161
BsiHKCI CYCGRG 1 cut(s) 4644
BsiSI CCGG 2 cut(s) 516, 2852
BslFI GGGAC 2 cut(s) 2080, 2647
BslI CCNNNNNNNGG 5 cut(s) 1509, 1867, 2030, 3715, 4757
BsmBI CGTCTC 2 cut(s) 3018, 3297
BsmFI GGGAC 2 cut(s) 2080, 2647
BsmI GAATGC 5 cut(s) 458, 994, 1699, 2076, 4775
BsnI GGCC 9 cut(s) 24, 272, 800, 887, 942, 2023, 2717, 2952, 3528
Bso31I GGTCTC 4 cut(s) 953, 3418, 3483, 3776
BsoBI CYCGRG 1 cut(s) 4644
Bsp119I TTCGAA 3 cut(s) 610, 1088, 4592
Bsp1286I GDGCHC 3 cut(s) 1516, 2578, 4161
Bsp1407I TGTACA 3 cut(s) 1661, 4333, 4743
Bsp1720I GCTNAGC 1 cut(s) 2577
Bsp19I CCATGG 1 cut(s) 3192
BspANI GGCC 9 cut(s) 24, 272, 800, 887, 942, 2023, 2717, 2952, 3528
BspCNI CTCAG 8 cut(s) 2590, 2764, 2803, 2986, 3111, 3183, 3793, 4516
BspDI ATCGAT 1 cut(s) 3067
BspFNI CGCG 3 cut(s) 2082, 3495, 4322
BspLI GGNNCC 7 cut(s) 73, 273, 352, 1951, 2119, 3544, 3635
BspMAI CTGCAG 2 cut(s) 3616, 4819
BspMI ACCTGC 3 cut(s) 1897, 3333, 3876
BspPI GGATC 8 cut(s) 916, 1581, 1628, 2528, 3377, 3905, 4273, 4775
BspT104I TTCGAA 3 cut(s) 610, 1088, 4592
BspT107I GGYRCC 3 cut(s) 71, 1949, 2117
BspTI CTTAAG 1 cut(s) 448
BspTNI GGTCTC 4 cut(s) 953, 3418, 3483, 3776
BsrDI GCAATG 4 cut(s) 1838, 2606, 3118, 4006
BsrGI TGTACA 3 cut(s) 1661, 4333, 4743
BssECI CCNNGG 6 cut(s) 354, 943, 1866, 3192, 3765, 4784
BssNAI GTATAC 2 cut(s) 3234, 4186
BssT1I CCWWGG 6 cut(s) 354, 943, 1866, 3192, 3765, 4784
Bst1107I GTATAC 2 cut(s) 3234, 4186
Bst2UI CCWGG 4 cut(s) 935, 3405, 3915, 4462
Bst6I CTCTTC 6 cut(s) 999, 3016, 3099, 3257, 4460, 4793
BstAFI CTTAAG 1 cut(s) 448
BstAUI TGTACA 3 cut(s) 1661, 4333, 4743
BstBAI YACGTR 1 cut(s) 3368
BstBI TTCGAA 3 cut(s) 610, 1088, 4592
BstC8I GCNNGC 6 cut(s) 1882, 2025, 2208, 3091, 3936, 4566
BstDSI CCRYGG 1 cut(s) 3192
BstFNI CGCG 3 cut(s) 2082, 3495, 4322
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 4 cut(s) 102, 2084, 2627, 3300
BstNI CCWGG 4 cut(s) 935, 3405, 3915, 4462
BstNSI RCATGY 3 cut(s) 1884, 2452, 3797
BstSCI CCNGG 5 cut(s) 933, 2851, 3403, 3913, 4460
BstSFI CTRYAG 4 cut(s) 690, 3237, 3612, 4815
BstUI CGCG 3 cut(s) 2082, 3495, 4322
BstX2I RGATCY 3 cut(s) 921, 1633, 4780
BstXI CCANNNNNNTGG 2 cut(s) 1196, 3393
BstYI RGATCY 3 cut(s) 921, 1633, 4780
BstZ17I GTATAC 2 cut(s) 3234, 4186
Bsu15I ATCGAT 1 cut(s) 3067
Bsu36I CCTNAGG 1 cut(s) 4101
BsuI GTATCC 2 cut(s) 2859, 3442
BsuRI GGCC 9 cut(s) 24, 272, 800, 887, 942, 2023, 2717, 2952, 3528
BsuTUI ATCGAT 1 cut(s) 3067
BtgI CCRYGG 1 cut(s) 3192
BtsI GCAGTG 3 cut(s) 1043, 1245, 3184
BveI ACCTGC 3 cut(s) 1897, 3333, 3876
Cac8I GCNNGC 6 cut(s) 1882, 2025, 2208, 3091, 3936, 4566
CaiI CAGNNNCTG 4 cut(s) 182, 2030, 2948, 4571
CfoI GCGC 4 cut(s) 102, 2084, 2627, 3300
Cfr13I GGNCC 8 cut(s) 271, 886, 1874, 2021, 3401, 3865, 4097, 4640
ClaI ATCGAT 1 cut(s) 3067
CseI GACGC 1 cut(s) 2245
DraI TTTAAA 3 cut(s) 331, 400, 831
DrdI GACNNNNNNGTC 1 cut(s) 1515
DriI GACNNNNNGTC 1 cut(s) 4646
DseDI GACNNNNNNGTC 1 cut(s) 1515
EaeI YGGCCR 1 cut(s) 2715
Eam1104I CTCTTC 6 cut(s) 999, 3016, 3099, 3257, 4460, 4793
Eam1105I GACNNNNNGTC 1 cut(s) 4646
EarI CTCTTC 6 cut(s) 999, 3016, 3099, 3257, 4460, 4793
Ecl136II GAGCTC 1 cut(s) 4159
Eco130I CCWWGG 6 cut(s) 354, 943, 1866, 3192, 3765, 4784
Eco147I AGGCCT 1 cut(s) 800
Eco24I GRGCYC 2 cut(s) 1516, 4161
Eco31I GGTCTC 4 cut(s) 953, 3418, 3483, 3776
Eco32I GATATC 1 cut(s) 3135
Eco47I GGWCC 5 cut(s) 1874, 3401, 3865, 4097, 4640
Eco53kI GAGCTC 1 cut(s) 4159
Eco57I CTGAAG 4 cut(s) 1659, 2894, 3693, 3927
Eco81I CCTNAGG 1 cut(s) 4101
Eco88I CYCGRG 1 cut(s) 4644
EcoICRI GAGCTC 1 cut(s) 4159
EcoO109I RGGNCCY 3 cut(s) 886, 4097, 4640
EcoRI GAATTC 2 cut(s) 1431, 2602
EcoRII CCWGG 4 cut(s) 933, 3403, 3913, 4460
EcoRV GATATC 1 cut(s) 3135
EcoT14I CCWWGG 6 cut(s) 354, 943, 1866, 3192, 3765, 4784
EcoT22I ATGCAT 4 cut(s) 6, 2566, 3799, 4533
EcoT38I GRGCYC 2 cut(s) 1516, 4161
ErhI CCWWGG 6 cut(s) 354, 943, 1866, 3192, 3765, 4784
Esp3I CGTCTC 2 cut(s) 3018, 3297
FalI AAGNNNNNCTT 4 cut(s) 446, 478, 3758, 3790
FaqI GGGAC 2 cut(s) 2080, 2647
FauNDI CATATG 2 cut(s) 953, 2985
FbaI TGATCA 3 cut(s) 64, 258, 1960
FblI GTMKAC 2 cut(s) 3233, 4185
FriOI GRGCYC 2 cut(s) 1516, 4161
GlaI GCGC 4 cut(s) 101, 2083, 2626, 3299
GsaI CCCAGC 1 cut(s) 2387
GsuI CTGGAG 1 cut(s) 1722
HaeII RGCGCY 1 cut(s) 103
HaeIII GGCC 9 cut(s) 24, 272, 800, 887, 942, 2023, 2717, 2952, 3528
HapII CCGG 2 cut(s) 516, 2852
HgaI GACGC 1 cut(s) 2245
HhaI GCGC 4 cut(s) 102, 2084, 2627, 3300
Hin6I GCGC 4 cut(s) 100, 2082, 2625, 3298
HinP1I GCGC 4 cut(s) 100, 2082, 2625, 3298
HincII GTYRAC 2 cut(s) 3924, 3949
HindII GTYRAC 2 cut(s) 3924, 3949
HindIII AAGCTT 3 cut(s) 1923, 2265, 3743
HpaI GTTAAC 2 cut(s) 3924, 3949
HpaII CCGG 2 cut(s) 516, 2852
HphI GGTGA 7 cut(s) 257, 499, 2603, 3227, 3863, 4085, 4319
HpyCH4IV ACGT 6 cut(s) 1462, 2971, 3027, 3199, 3367, 4739
HpySE526I ACGT 6 cut(s) 1462, 2971, 3027, 3199, 3367, 4739
HspAI GCGC 4 cut(s) 100, 2082, 2625, 3298
Ksp22I TGATCA 3 cut(s) 64, 258, 1960
KspAI GTTAAC 2 cut(s) 3924, 3949
MaeII ACGT 6 cut(s) 1462, 2971, 3027, 3199, 3367, 4739
MfeI CAATTG 2 cut(s) 1943, 4206
MflI RGATCY 3 cut(s) 921, 1633, 4780
MhlI GDGCHC 3 cut(s) 1516, 2578, 4161
MlsI TGGCCA 1 cut(s) 2717
MluNI TGGCCA 1 cut(s) 2717
MlyI GAGTC 5 cut(s) 2207, 2785, 3746, 4656, 4805
MmeI TCCRAC 2 cut(s) 3487, 3843
Mox20I TGGCCA 1 cut(s) 2717
Mph1103I ATGCAT 4 cut(s) 6, 2566, 3799, 4533
MroXI GAANNNNTTC 4 cut(s) 846, 1176, 1376, 4314
MscI TGGCCA 1 cut(s) 2717
MslI CAYNNNNRTG 4 cut(s) 1229, 1541, 3172, 4068
Msp20I TGGCCA 1 cut(s) 2717
MspA1I CMGCKG 3 cut(s) 2387, 2771, 4339
MspCI CTTAAG 1 cut(s) 448
MspI CCGG 2 cut(s) 516, 2852
MspR9I CCNGG 5 cut(s) 935, 2853, 3405, 3915, 4462
MunI CAATTG 2 cut(s) 1943, 4206
Mva1269I GAATGC 5 cut(s) 458, 994, 1699, 2076, 4775
MvaI CCWGG 4 cut(s) 935, 3405, 3915, 4462
MvnI CGCG 3 cut(s) 2082, 3495, 4322
NciI CCSGG 1 cut(s) 2853
NcoI CCATGG 1 cut(s) 3192
NdeI CATATG 2 cut(s) 953, 2985
NlaIV GGNNCC 7 cut(s) 73, 273, 352, 1951, 2119, 3544, 3635
NmuCI GTSAC 8 cut(s) 245, 1243, 1751, 2591, 2759, 3518, 4073, 4210
NsiI ATGCAT 4 cut(s) 6, 2566, 3799, 4533
NspI RCATGY 3 cut(s) 1884, 2452, 3797
NspV TTCGAA 3 cut(s) 610, 1088, 4592
PaeI GCATGC 1 cut(s) 1884
PaeR7I CTCGAG 1 cut(s) 4644
PceI AGGCCT 1 cut(s) 800
PciI ACATGT 1 cut(s) 2448
PctI GAATGC 5 cut(s) 458, 994, 1699, 2076, 4775
PdmI GAANNNNTTC 4 cut(s) 846, 1176, 1376, 4314
PflMI CCANNNNNTGG 2 cut(s) 1867, 2030
PfoI TCCNGGA 1 cut(s) 3403
PleI GAGTC 5 cut(s) 2206, 2784, 3745, 4655, 4805
PpsI GAGTC 5 cut(s) 2206, 2784, 3745, 4655, 4805
Ppu21I YACGTR 1 cut(s) 3368
PpuMI RGGWCCY 2 cut(s) 4097, 4640
PscI ACATGT 1 cut(s) 2448
PsiI TTATAA 4 cut(s) 753, 1541, 2136, 4284
Psp124BI GAGCTC 1 cut(s) 4161
Psp1406I AACGTT 2 cut(s) 3199, 4739
Psp5II RGGWCCY 2 cut(s) 4097, 4640
Psp6I CCWGG 4 cut(s) 933, 3403, 3913, 4460
PspFI CCCAGC 1 cut(s) 2383
PspGI CCWGG 4 cut(s) 933, 3403, 3913, 4460
PspN4I GGNNCC 7 cut(s) 73, 273, 352, 1951, 2119, 3544, 3635
PspPI GGNCC 8 cut(s) 271, 886, 1874, 2021, 3401, 3865, 4097, 4640
PspPPI RGGWCCY 2 cut(s) 4097, 4640
PspXI VCTCGAGB 1 cut(s) 4644
PstI CTGCAG 2 cut(s) 3616, 4819
PstNI CAGNNNCTG 4 cut(s) 182, 2030, 2948, 4571
PsuI RGATCY 3 cut(s) 921, 1633, 4780
PvuII CAGCTG 2 cut(s) 2387, 4339
RseI CAYNNNNRTG 4 cut(s) 1229, 1541, 3172, 4068
SacI GAGCTC 1 cut(s) 4161
Sau96I GGNCC 8 cut(s) 271, 886, 1874, 2021, 3401, 3865, 4097, 4640
ScaI AGTACT 3 cut(s) 1324, 1775, 2816
SchI GAGTC 5 cut(s) 2207, 2785, 3746, 4656, 4805
ScrFI CCNGG 5 cut(s) 935, 2853, 3405, 3915, 4462
SduI GDGCHC 3 cut(s) 1516, 2578, 4161
SfcI CTRYAG 4 cut(s) 690, 3237, 3612, 4815
Sfr274I CTCGAG 1 cut(s) 4644
SfuI TTCGAA 3 cut(s) 610, 1088, 4592
SinI GGWCC 5 cut(s) 1874, 3401, 3865, 4097, 4640
SlaI CTCGAG 1 cut(s) 4644
SmiMI CAYNNNNRTG 4 cut(s) 1229, 1541, 3172, 4068
SmlI CTYRAG 7 cut(s) 448, 1052, 3653, 3966, 4226, 4427, 4644
SmoI CTYRAG 7 cut(s) 448, 1052, 3653, 3966, 4226, 4427, 4644
SphI GCATGC 1 cut(s) 1884
SseBI AGGCCT 1 cut(s) 800
SspI AATATT 1 cut(s) 2227
SstI GAGCTC 1 cut(s) 4161
StuI AGGCCT 1 cut(s) 800
StyD4I CCNGG 5 cut(s) 933, 2851, 3403, 3913, 4460
StyI CCWWGG 6 cut(s) 354, 943, 1866, 3192, 3765, 4784
TaiI ACGT 6 cut(s) 1465, 2974, 3030, 3202, 3370, 4742
TaqII GACCGA 1 cut(s) 1862
TauI GCSGC 5 cut(s) 77, 80, 1856, 2872, 3941
TseFI GTSAC 8 cut(s) 245, 1243, 1751, 2591, 2759, 3518, 4073, 4210
Tsp45I GTSAC 8 cut(s) 245, 1243, 1751, 2591, 2759, 3518, 4073, 4210
TspGWI ACGGA 2 cut(s) 1721, 4169
Van91I CCANNNNNTGG 2 cut(s) 1867, 2030
Vha464I CTTAAG 1 cut(s) 448
VpaK11BI GGWCC 5 cut(s) 1874, 3401, 3865, 4097, 4640
XapI RAATTY 9 cut(s) 395, 1013, 1102, 1302, 1431, 2138, 2336, 2602, 4026
XbaI TCTAGA 2 cut(s) 982, 3151
XceI RCATGY 3 cut(s) 1884, 2452, 3797
XcmI CCANNNNNNNNNTGG 2 cut(s) 2828, 3199
XhoI CTCGAG 1 cut(s) 4644
XmaJI CCTAGG 1 cut(s) 4784
XmiI GTMKAC 2 cut(s) 3233, 4185
XmnI GAANNNNTTC 4 cut(s) 846, 1176, 1376, 4314
ZrmI AGTACT 3 cut(s) 1324, 1775, 2816
Zsp2I ATGCAT 4 cut(s) 6, 2566, 3799, 4533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.