FvH4_2g30780

Belongs to the helicase family. Dicer subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001909
Physical Location & Seq
Reverse (-)
23755878 .. 23765432
9555 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30780.t4

Sequence Viewer

Length: 4881 bp
ATGCATTTGCCAGGGGAAGAAGGCCACAATCCCCTGAAACGCAGCTTGTCTGACATGACTGATGGTCATGGTGCTGCTGCCGATCAAGAAGGTCCAAAGCGCTTCAGTCCCAGAAGGTATCAGACACAAGTGTATGAGGTTGCTAGGAGGAGGAACACAATAGCTGTGATGGAGACTGGTACTGGGAAGACAATGATAGCTGTGATGCTCATTGATGAAATTGGTCAAGCTATCAAGTCATCTGGTGACAACAAATTGATCATCTTCTTGGCCCCAACTGTTCATCTTGTTCATCAGCAATTTGGGGTTATCAAAGAACACACCAGTTTTGAAGTGGAGGAGTATTATGGAGCCAAGGGAGTTGATGCATGGGATAAGGAGCATTGGGAAAAGGAATTTAAAAACCATGATATACTGGTTATGACACCCCAGATTCTTTTGGATGCCTTAAGAAAGGCATTCTTGACCATGGAAGCAGTATGCTTGTTGATCATGGATGAGTGCCACCGGGCTACCGGAAATCATCCTTATACGAAGATCATGAAGGAATTTTATCACAGGTCTAGTATGAAGCCAAAAGTTTTTGGAATGACGGCTTCACCTGTAATTCGGAAAGGTGTCTCATCCCCAATGGACTGTGAAGATCAAATATCAGAACTTGAAAGCATTTTGGATTCCCAGATATATACTGTTGAGGATAAAACAGAGATGGAAGTGTATGCCCCTACTGCGAAAGAAACTCGTAGATTTTATAACCCAACATGGTTTTCAAGTTTGGATTTGAAAGAAAAGATCAAGGCTTCTTGGTCGAAGAATGATGCTTTGTTTTTAACACTGCAAGAATCAATACAAAGCAATTTTAAAGATTTGGAGGACAAAGTTAAGACTCTGCGGAGACGATTGTCTAATGACTATGAAAAGATCCTATATTGCCTGGACAACCTTGGTCTTATATGTGCTTATGAGGCCGTGAAGGTCTGTCTAGAGAACGCTCCTACTAGCGAAGAGTGTGAAGTTTATAGAGAAAGTTCTATACGGTGCAGATATTTTCTCGAGGAAGTTGTTGGTATAATAAAGCATTCTCTTTTGAACGGTGTTGAAATTTTTTTGGATGTTGGGTTTGACTATTTGAAGGCAGTAGATTTGGGCTACATATCTCCAAAGTTAAATGAACTTGTTCAGCTTTTCCATTCATTTGGAATATCTAGGGATCTACTGTGCCTCATCTTTGTGGATAGAATTATCACAGCTAAAGTGATTGAAAGGTTCATGAGAAAAGTTACCTCCTTGTCTCATTTCAAGATTTCATATTTGACTGGGAGCACTACCTCAGTTGATGCTCTGACACCGAAAATACAAAAGGAGACATTGAAGTTATTCAGCTCTGGGGAGGTCAATTTATTATTTTCTACGGATGTAGTTGAGGAGGGAATTCATGTTCCAAACTGCTCCTGTGTGATACGTTTTGATTTGCCAAAGACAGTGCGTAGTTATGTACAGTCTAGAGGACGGGCTCGTCAAGATAACTCTCAATTCATTATAATGCTAGAGAGAGGAAATGAGCAACAGAGGGATCAACTATATGAGATTATCAAGAGCGAAGGTTTGATGACAAATACAGCTCTAAATAGAGATCCTGAAGAAATTGTTTTGAAACCATGTACACTTGATGAAATAAGTGAATATATTGTGGATGCCACTGGAGCATCGGTTACAGCAGATTCCAGTGTCCGTCTCATACATAAGTATTGTGACACACTCCCCAGAGACAAGTACTTTATTCCAAGGCCAGCTTTTCAATTTTCATATCTCGGAGATTCTTATGAATGTAAGATTACATTGCCTCCAAATGCGGCTTTCCAAACCTTGGTTGGTCCTGCATGCAAAAACTCTGCTTTATCAAAGCAGGTTGTATGCCTAGAAGCTTGTAAAAAGCTGCATCAATTGGGTGCCTTGAATGATCATCTTCTCCCATCCACTGATAAGCCTTTGGAGAAAGATATAATTACAGAGAGCAAAGGGCCAGCGTCTGGTGCGGGAACAACCAAAAGAAAGGAGCTACATGGGACAACTAGCATTCGCGCTCTGTCGGGAACATGGGCAGAAAAACTTGATGGTGCTGTTTTTTATGCCTATAAATTTGACTTCTCCTGTGATATTGTCACCGAGCTATATTCCGGATTAATTCTTCTGATTGAGACGGAGCTTGCTAAGGATGTGGGGAATATTGGATTGGAGCTTTACTTGGTTTCAAAGAAAGTTAAAGCTTCTGTTTCTTCCTGTGGGCAAGTTCGTTTGGATAGAGAGCAGATGGCAAAAGCAAAGCTCTTTCAGGAATTTTTCTTTAATGGATTGTTTGGGAAATTGTTTCATCGAATCAACCCAGCTGAAAAGCAGAGAGAATTTTTACTTAAGAAAGAAACAAGAAAGCTGTGGAGGCAATCATACATGTATTTGGTTCTTCCACTTGAAACATTTAATGATTCAAATAATGATTCTTGGAAAATAAATTGGGGAGGGATCAATTCTTGTGTTTCTGTGGTAGAGTTCTTGAAGCAAAATGCATTATTGGGGGCTCAGCATTGTAATGGTGATGCAAGGAGATCATTGCAAAACAGAACTGGCCCATCTGGGACTGGATGCAATGGTTCACAGATATTCCACTTTGCTGATTCTTCTGTTGATGAAAACAGTTTCAAAGATATGGTGGTAGTGGCCATCCACACTGGAAAAATATATTCTGTTTTTGAGGTTTTGAGTGACACATCTGCTGAGAGCCCTTTTGACACATCAGAGTACAATAACTACGCTGAGTACTTCAACAAAAAATATGGGATCCTGCTCATGTATCCAGGTCAGCCTCTTTTGCGGCTGAAGCAAAATCATAATCCACACAATCTTCTTGTGAACTTCAATGGCGAAGGTGGTAGTGGTATGACATCAGAATCTGGCCTTGTTAATGGAAAGGAACGTATGTACGCCCATATGCCTCCTGAGCTTCTAGCCTGTACTGGTGTGAGAAGAGAGGTTTTGAAGTCATTTTATCTAATACCATCATTGATTCATCGATTGGAATCCTTGATGTTAGCTAGCCAACTCAGAGAAGAGATTAGTAGTCATCCTAGCAGCTTACAGATATCAAGCTCGTTGATTCTAGAAGCACTCACATCACTTAGATGCAGTGAAGATTTTTCCATGGAGCGTTTGGAACTGCTTGGTGATTCAGTCCTAAAGTATACTGTAGGCTGCCACCTCTTCCTAAAATATCCCGAAAAACATGATGGACAATTATCGTCTCTGCGCCAGTCAGTAATTTGTAACGCAAACCTGCATAGATTGGGAATAGATCGAAACTTACAGGGATATGTACGTGACGGAGCTTTTGATCCGCGCCGTTGGGTTGGTCCAGGACAGCTCTCTATACGACCTGTTCCTTGTGAGTGCGGAGTGGATACCTTAGAAGTTCCAGTAGATTGCAAGTTTCAAACTAGAGACCCCGGAATTGTGGTTGGAAAGTGCTGTGACATGGGACATAGATGGATGGGTTCCAAAACAATCTCTGATTGTGTTGAAGCCCTTATAGGAGCTTACTATGTAGTTGGTGGTTTATCTGCTGCTGTTCATTTTATGAAGTGGCTCAAAATTGATTTAGAGCTTGAGCCATCCTTGGTTTCTGAAGCAATTGCTACTGCATCACTTCATTCCTGCAATCCAATGGTGGATGACATTGGAATCCTTGAGTCAAAGCTTCGTTATGTATTTTCTACCAAGGGTCTCTTACAGGAAGCCATAACACATGCATCTGAGCAAGATTCAAAACTTGGCTATTCTTACGAGAGACTAGAATATCTTGGTGATTGTGTGTTGGACCTACTTATCACACGGTATCTCTATCAGAGCCACAAAGATATCGATCCAGGAGAGTTAACTGATTTGCGTGCTGCTTCAGTTAACAATGAAAACTTTGCTCAAGCTGCTGTAAGACACAACCTTCAACAGCATCTACAACATTGCTCTGGGTTACTTCAGAGTCAAATAACAGAATATGCAAAGTCCCTCTCTGAACATGACATTGGTAGCAAACTTCAAGTCCCAAAAGGCCCTAAGGCTCTTGGAGACATGTTGGAAAGTATTGCAGGGGCAATACTAATTGATACGAAGCTCAACCTTGACGAAGTGTGGAGAGTATACAAACCACTGTTATCTCCAATTGTGACTCCTGATAAACTTGAGCTGCCTCCCTTGCGTGAACTGATTGAATTATGTGGCTCACTTGGGTACTTCCATAAAGAACAAATTGTAAAGAAGGATGAAACAGTTCACGCAGAGCTTAGTGTACAGCTGAAAGATGTCCTGTTGATTGGAAAGGGTATCGATCGAAGTAAAAAGACCGCAAAAGGAGAAGCAGCTCATCATTTATTGAAGGAGCTTGAGAAAAGAGGTATTGCACACTCTGGGTCTGCCAGAAAGAGAAAACTGGGTGCTGAACAAGTTGATGATACATCTCCAACAAAGCAAAAGAAAACTGAGATGCATCTGCCTGTGGATACAACTGAACTTTCTTCACCAGCCACTGTCAGAAAAATAAGTAGTCTGAAAAATGACATCCCAGTTATAAAACCAATCAACATGAAGAAAGGAGGACCTCGGGTCTCTCTTTTTGAGCTGTGCAAACAACAACAGTGGCAAATGCCTACCTTCAAACCAACAGAAAAGGATTTCAGTTTCACATCAACAATAACGTTGTACATACCCAATTTGGGCAACATTGAATGCACAGGAGATCCTAGGTCAGACAAGAAGAGTTCACTAGACTCTGCAGCACTTCTCATGCTTTATGATCTCGAACGGCAGGGTAGAATTATCATCGGTGGATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009892 GO:0009987 GO:0010033 GO:0010216 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0031974 GO:0031981 GO:0032296 GO:0032991 GO:0034641 GO:0035194 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051214 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0098586 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko05206 map05206
Pfam Domains
Protein Families
DEAD Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1627

Amino Acids

182.69

Weight (kDa)

6.44

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 49 - 165 4.1e-17 Helicase conserved C-terminal domain
Dicer_dimer PF03368 236 - 322 7.2e-24 Dicer dimerisation domain
PAZ PF02170 556 - 662 3.6e-10 PAZ domain
Ribonucleas_3_3 PF14622 725 - 784 7.4e-07 Ribonuclease-III-like
Ribonuclease_3 PF00636 727 - 862 1.2e-16 Ribonuclease III domain
Ribonucleas_3_3 PF14622 918 - 1045 3e-13 Ribonuclease-III-like
Ribonuclease_3 PF00636 939 - 1046 3.1e-20 Ribonuclease III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 753, 1541, 4616
AasI GACNNNNNNGTC 1 cut(s) 1515
Acc36I ACCTGC 2 cut(s) 1897, 3336
AccB1I GGYRCC 1 cut(s) 1949
AccB7I CCANNNNNTGG 2 cut(s) 1867, 2030
AccI GTMKAC 2 cut(s) 3236, 4188
AccII CGCG 2 cut(s) 2082, 3391
AccIII TCCGGA 1 cut(s) 2177
AciI CCGC 7 cut(s) 892, 1853, 2036, 2869, 3389, 3444, 4392
AclI AACGTT 1 cut(s) 4742
AcoI YGGCCR 1 cut(s) 2715
AcsI RAATTY 7 cut(s) 395, 548, 1101, 1431, 2138, 2336, 2402
AcuI CTGAAG 6 cut(s) 88, 1659, 2894, 3696, 3930, 4010
AfeI AGCGCT 1 cut(s) 101
AfiI CCNNNNNNNGG 3 cut(s) 1867, 2030, 4760
AflII CTTAAG 2 cut(s) 448, 2411
AflIII ACRYGT 2 cut(s) 2448, 4119
AhdI GACNNNNNGTC 2 cut(s) 63, 4649
AjnI CCWGG 5 cut(s) 10, 933, 2851, 3406, 3916
AjuI GAANNNNNNNTTGG 6 cut(s) 751, 783, 2216, 2248, 4056, 4088
AloI GAACNNNNNNTCC 2 cut(s) 1422, 1454
Alw21I GWGCWC 1 cut(s) 1325
AlwNI CAGNNNCTG 3 cut(s) 2030, 2948, 4574
Ama87I CYCGRG 2 cut(s) 1052, 4647
Aor13HI TCCGGA 1 cut(s) 2177
Aor51HI AGCGCT 1 cut(s) 101
AoxI GGCC 9 cut(s) 22, 270, 966, 1787, 2021, 2623, 2715, 2950, 4099
ApoI RAATTY 7 cut(s) 395, 548, 1101, 1431, 2138, 2336, 2402
ArsI GACNNNNNNTTYG 4 cut(s) 3365, 3397, 4074, 4106
AseI ATTAAT 1 cut(s) 2183
Asp700I GAANNNNTTC 3 cut(s) 1176, 1376, 4317
AspA2I CCTAGG 1 cut(s) 4787
AspLEI GCGC 4 cut(s) 102, 2084, 3303, 3393
AspS9I GGNCC 9 cut(s) 92, 271, 1874, 2021, 2624, 3404, 3868, 4100, 4643
AsuC2I CCSGG 2 cut(s) 509, 3498
AsuHPI GGTGA 7 cut(s) 257, 591, 2155, 2603, 3230, 3866, 4557
AsuNHI GCTAGC 1 cut(s) 3089
AvaI CYCGRG 2 cut(s) 1052, 4647
AvaII GGWCC 5 cut(s) 92, 1874, 3404, 3868, 4643
AvrII CCTAGG 1 cut(s) 4787
AxyI CCTNAGG 1 cut(s) 4104
BalI TGGCCA 1 cut(s) 2717
BamHI GGATCC 1 cut(s) 2835
BanI GGYRCC 1 cut(s) 1949
BanII GRGCYC 3 cut(s) 1516, 2578, 2780
BbsI GAAGAC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 1325
BceAI ACGGC 4 cut(s) 609, 953, 3378, 4865
BciT130I CCWGG 5 cut(s) 12, 935, 2853, 3408, 3918
BciVI GTATCC 3 cut(s) 2859, 3445, 4540
BclI TGATCA 3 cut(s) 258, 489, 1960
BcnI CCSGG 2 cut(s) 509, 3498
BfmI CTRYAG 2 cut(s) 3240, 4818
BfoI RGCGCY 1 cut(s) 103
BfrI CTTAAG 2 cut(s) 448, 2411
BfuAI ACCTGC 2 cut(s) 1897, 3336
BfuI GTATCC 3 cut(s) 2859, 3445, 4540
BlnI CCTAGG 1 cut(s) 4787
BlpI GCTNAGC 1 cut(s) 2577
BmcAI AGTACT 2 cut(s) 1775, 2816
Bme1390I CCNGG 7 cut(s) 12, 509, 935, 2853, 3408, 3498, 3918
Bme18I GGWCC 5 cut(s) 92, 1874, 3404, 3868, 4643
BmeRI GACNNNNNGTC 2 cut(s) 63, 4649
BmeT110I CYCGRG 2 cut(s) 1052, 4647
BmgT120I GGNCC 9 cut(s) 92, 271, 1874, 2021, 2624, 3404, 3868, 4100, 4643
BmiI GGNNCC 5 cut(s) 273, 352, 1951, 2837, 3547
BmrFI CCNGG 7 cut(s) 12, 509, 935, 2853, 3408, 3498, 3918
BmrI ACTGGG 4 cut(s) 192, 1326, 4487, 4604
BmtI GCTAGC 1 cut(s) 3093
BmuI ACTGGG 4 cut(s) 192, 1326, 4487, 4604
BoxI GACNNNNGTC 1 cut(s) 901
BpiI GAAGAC 1 cut(s) 194
BpmI CTGGAG 1 cut(s) 1722
Bpu10I CCTNAGC 2 cut(s) 2211, 2994
Bpu1102I GCTNAGC 1 cut(s) 2577
BpuEI CTTGAG 5 cut(s) 3677, 3758, 3954, 4250, 4451
BpuMI CCSGG 2 cut(s) 509, 3498
Bsa29I ATCGAT 3 cut(s) 3067, 3912, 4374
BsaAI YACGTR 1 cut(s) 3371
BsaBI GATNNNNATC 2 cut(s) 3073, 3912
BsaI GGTCTC 3 cut(s) 3486, 3779, 4657
BsaWI WCCGGW 2 cut(s) 515, 2177
BsaXI ACNNNNNCTCC 4 cut(s) 1695, 1725, 1828, 1858
Bsc4I CCNNNNNNNGG 3 cut(s) 1867, 2030, 4760
Bse21I CCTNAGG 1 cut(s) 4104
Bse3DI GCAATG 4 cut(s) 1838, 2606, 2650, 4009
Bse8I GATNNNNATC 2 cut(s) 3073, 3912
BseAI TCCGGA 1 cut(s) 2177
BseBI CCWGG 5 cut(s) 12, 935, 2853, 3408, 3918
BseCI ATCGAT 3 cut(s) 3067, 3912, 4374
BseJI GATNNNNATC 2 cut(s) 3073, 3912
BseLI CCNNNNNNNGG 3 cut(s) 1867, 2030, 4760
BseMI GCAATG 4 cut(s) 1838, 2606, 2650, 4009
BseMII CTCAG 8 cut(s) 1344, 2591, 2763, 2802, 2985, 3112, 3795, 4518
BseRI GAGGAG 3 cut(s) 163, 353, 1439
BseYI CCCAGC 1 cut(s) 2383
BsgI GTGCAG 1 cut(s) 1060
Bsh1236I CGCG 2 cut(s) 2082, 3391
Bsh1285I CGRYCG 1 cut(s) 4378
BshFI GGCC 9 cut(s) 24, 272, 968, 1789, 2023, 2625, 2717, 2952, 4101
BshNI GGYRCC 1 cut(s) 1949
BshVI ATCGAT 3 cut(s) 3067, 3912, 4374
BsiEI CGRYCG 1 cut(s) 4378
BsiHKAI GWGCWC 1 cut(s) 1325
BsiHKCI CYCGRG 2 cut(s) 1052, 4647
BsiSI CCGG 4 cut(s) 508, 516, 2178, 3498
BslFI GGGAC 6 cut(s) 93, 2080, 2647, 3543, 4039, 4076
BslI CCNNNNNNNGG 3 cut(s) 1867, 2030, 4760
BsmBI CGTCTC 4 cut(s) 889, 1739, 2192, 3300
BsmFI GGGAC 6 cut(s) 93, 2080, 2647, 3543, 4039, 4076
BsmI GAATGC 4 cut(s) 458, 1078, 2076, 4778
BsnI GGCC 9 cut(s) 24, 272, 968, 1789, 2023, 2625, 2717, 2952, 4101
Bso31I GGTCTC 3 cut(s) 3486, 3779, 4657
BsoBI CYCGRG 2 cut(s) 1052, 4647
Bsp1286I GDGCHC 4 cut(s) 1325, 1516, 2578, 2780
Bsp13I TCCGGA 1 cut(s) 2177
Bsp1407I TGTACA 4 cut(s) 1495, 1661, 4336, 4746
Bsp1720I GCTNAGC 1 cut(s) 2577
Bsp19I CCATGG 2 cut(s) 468, 3195
BspACI CCGC 7 cut(s) 892, 1853, 2036, 2869, 3389, 3444, 4392
BspANI GGCC 9 cut(s) 24, 272, 968, 1789, 2023, 2625, 2717, 2952, 4101
BspCNI CTCAG 8 cut(s) 1343, 2590, 2764, 2803, 2986, 3111, 3796, 4519
BspDI ATCGAT 3 cut(s) 3067, 3912, 4374
BspEI TCCGGA 1 cut(s) 2177
BspFNI CGCG 2 cut(s) 2082, 3391
BspHI TCATGA 2 cut(s) 540, 1269
BspLI GGNNCC 5 cut(s) 273, 352, 1951, 2837, 3547
BspMAI CTGCAG 1 cut(s) 4822
BspMI ACCTGC 2 cut(s) 1897, 3336
BspOI GCTAGC 1 cut(s) 3093
BspT107I GGYRCC 1 cut(s) 1949
BspTI CTTAAG 2 cut(s) 448, 2411
BspTNI GGTCTC 3 cut(s) 3486, 3779, 4657
BsrDI GCAATG 4 cut(s) 1838, 2606, 2650, 4009
BsrGI TGTACA 4 cut(s) 1495, 1661, 4336, 4746
BssNAI GTATAC 2 cut(s) 3237, 4189
BssT1I CCWWGG 9 cut(s) 354, 468, 943, 1784, 1866, 3195, 3666, 3768, 4787
Bst1107I GTATAC 2 cut(s) 3237, 4189
Bst2UI CCWGG 5 cut(s) 12, 935, 2853, 3408, 3918
Bst6I CTCTTC 5 cut(s) 999, 3016, 3099, 3260, 4796
BstAFI CTTAAG 2 cut(s) 448, 2411
BstAUI TGTACA 4 cut(s) 1495, 1661, 4336, 4746
BstBAI YACGTR 1 cut(s) 3371
BstC8I GCNNGC 6 cut(s) 1791, 1882, 2025, 2208, 3091, 3939
BstDSI CCRYGG 2 cut(s) 468, 3195
BstFNI CGCG 2 cut(s) 2082, 3391
BstH2I RGCGCY 1 cut(s) 103
BstHHI GCGC 4 cut(s) 102, 2084, 3303, 3393
BstMCI CGRYCG 1 cut(s) 4378
BstNI CCWGG 5 cut(s) 12, 935, 2853, 3408, 3918
BstNSI RCATGY 4 cut(s) 1884, 2452, 3800, 4123
BstPAI GACNNNNGTC 1 cut(s) 901
BstSCI CCNGG 7 cut(s) 10, 507, 933, 2851, 3406, 3496, 3916
BstSFI CTRYAG 2 cut(s) 3240, 4818
BstUI CGCG 2 cut(s) 2082, 3391
BstV2I GAAGAC 1 cut(s) 194
BstX2I RGATCY 5 cut(s) 921, 1210, 1633, 2835, 4783
BstXI CCANNNNNNTGG 1 cut(s) 1196
BstYI RGATCY 5 cut(s) 921, 1210, 1633, 2835, 4783
BstZ17I GTATAC 2 cut(s) 3237, 4189
Bsu15I ATCGAT 3 cut(s) 3067, 3912, 4374
Bsu36I CCTNAGG 1 cut(s) 4104
BsuI GTATCC 3 cut(s) 2859, 3445, 4540
BsuRI GGCC 9 cut(s) 24, 272, 968, 1789, 2023, 2625, 2717, 2952, 4101
BsuTUI ATCGAT 3 cut(s) 3067, 3912, 4374
BtgI CCRYGG 2 cut(s) 468, 3195
BtsI GCAGTG 2 cut(s) 833, 3187
BveI ACCTGC 2 cut(s) 1897, 3336
Cac8I GCNNGC 6 cut(s) 1791, 1882, 2025, 2208, 3091, 3939
CaiI CAGNNNCTG 3 cut(s) 2030, 2948, 4574
CciI TCATGA 2 cut(s) 540, 1269
CfoI GCGC 4 cut(s) 102, 2084, 3303, 3393
Cfr13I GGNCC 9 cut(s) 92, 271, 1874, 2021, 2624, 3404, 3868, 4100, 4643
ClaI ATCGAT 3 cut(s) 3067, 3912, 4374
CseI GACGC 1 cut(s) 2016
CspCI CAANNNNNGTGG 2 cut(s) 4664, 4699
DraI TTTAAA 2 cut(s) 400, 862
DrdI GACNNNNNNGTC 1 cut(s) 1515
DriI GACNNNNNGTC 2 cut(s) 63, 4649
DseDI GACNNNNNNGTC 1 cut(s) 1515
EaeI YGGCCR 1 cut(s) 2715
Eam1104I CTCTTC 5 cut(s) 999, 3016, 3099, 3260, 4796
Eam1105I GACNNNNNGTC 2 cut(s) 63, 4649
EarI CTCTTC 5 cut(s) 999, 3016, 3099, 3260, 4796
Eco130I CCWWGG 9 cut(s) 354, 468, 943, 1784, 1866, 3195, 3666, 3768, 4787
Eco24I GRGCYC 3 cut(s) 1516, 2578, 2780
Eco31I GGTCTC 3 cut(s) 3486, 3779, 4657
Eco32I GATATC 2 cut(s) 3138, 3910
Eco47I GGWCC 5 cut(s) 92, 1874, 3404, 3868, 4643
Eco47III AGCGCT 1 cut(s) 101
Eco57I CTGAAG 6 cut(s) 88, 1659, 2894, 3696, 3930, 4010
Eco81I CCTNAGG 1 cut(s) 4104
Eco88I CYCGRG 2 cut(s) 1052, 4647
EcoO109I RGGNCCY 2 cut(s) 4100, 4643
EcoRI GAATTC 1 cut(s) 1431
EcoRII CCWGG 5 cut(s) 10, 933, 2851, 3406, 3916
EcoRV GATATC 2 cut(s) 3138, 3910
EcoT14I CCWWGG 9 cut(s) 354, 468, 943, 1784, 1866, 3195, 3666, 3768, 4787
EcoT22I ATGCAT 5 cut(s) 6, 370, 2566, 3802, 4536
EcoT38I GRGCYC 3 cut(s) 1516, 2578, 2780
ErhI CCWWGG 9 cut(s) 354, 468, 943, 1784, 1866, 3195, 3666, 3768, 4787
Esp3I CGTCTC 4 cut(s) 889, 1739, 2192, 3300
FalI AAGNNNNNCTT 6 cut(s) 446, 478, 1777, 1809, 3761, 3793
FaqI GGGAC 6 cut(s) 93, 2080, 2647, 3543, 4039, 4076
FauI CCCGC 1 cut(s) 2029
FauNDI CATATG 1 cut(s) 2985
FbaI TGATCA 3 cut(s) 258, 489, 1960
FblI GTMKAC 2 cut(s) 3236, 4188
FriOI GRGCYC 3 cut(s) 1516, 2578, 2780
GlaI GCGC 4 cut(s) 101, 2083, 3302, 3392
GsaI CCCAGC 1 cut(s) 2387
GsuI CTGGAG 1 cut(s) 1722
HaeII RGCGCY 1 cut(s) 103
HaeIII GGCC 9 cut(s) 24, 272, 968, 1789, 2023, 2625, 2717, 2952, 4101
HapII CCGG 4 cut(s) 508, 516, 2178, 3498
HgaI GACGC 1 cut(s) 2016
HhaI GCGC 4 cut(s) 102, 2084, 3303, 3393
Hin6I GCGC 4 cut(s) 100, 2082, 3301, 3391
HinP1I GCGC 4 cut(s) 100, 2082, 3301, 3391
HincII GTYRAC 2 cut(s) 3927, 3952
HindII GTYRAC 2 cut(s) 3927, 3952
HindIII AAGCTT 3 cut(s) 1923, 2265, 3746
HpaI GTTAAC 2 cut(s) 3927, 3952
HpaII CCGG 4 cut(s) 508, 516, 2178, 3498
HphI GGTGA 7 cut(s) 257, 591, 2155, 2603, 3230, 3866, 4557
HpyCH4IV ACGT 4 cut(s) 1462, 2971, 3370, 4742
HpySE526I ACGT 4 cut(s) 1462, 2971, 3370, 4742
HspAI GCGC 4 cut(s) 100, 2082, 3301, 3391
Kpn2I TCCGGA 1 cut(s) 2177
Ksp22I TGATCA 3 cut(s) 258, 489, 1960
KspAI GTTAAC 2 cut(s) 3927, 3952
MaeII ACGT 4 cut(s) 1462, 2971, 3370, 4742
MfeI CAATTG 3 cut(s) 1943, 3681, 4209
MflI RGATCY 5 cut(s) 921, 1210, 1633, 2835, 4783
MhlI GDGCHC 4 cut(s) 1325, 1516, 2578, 2780
MlsI TGGCCA 1 cut(s) 2717
MluNI TGGCCA 1 cut(s) 2717
MlyI GAGTC 5 cut(s) 880, 3749, 4039, 4210, 4808
MmeI TCCRAC 4 cut(s) 3490, 3846, 4104, 4532
Mox20I TGGCCA 1 cut(s) 2717
Mph1103I ATGCAT 5 cut(s) 6, 370, 2566, 3802, 4536
MroI TCCGGA 1 cut(s) 2177
MroXI GAANNNNTTC 3 cut(s) 1176, 1376, 4317
MscI TGGCCA 1 cut(s) 2717
MslI CAYNNNNRTG 4 cut(s) 1229, 1541, 2586, 3175
Msp20I TGGCCA 1 cut(s) 2717
MspA1I CMGCKG 2 cut(s) 2387, 4342
MspCI CTTAAG 2 cut(s) 448, 2411
MspI CCGG 4 cut(s) 508, 516, 2178, 3498
MspR9I CCNGG 7 cut(s) 12, 509, 935, 2853, 3408, 3498, 3918
MunI CAATTG 3 cut(s) 1943, 3681, 4209
Mva1269I GAATGC 4 cut(s) 458, 1078, 2076, 4778
MvaI CCWGG 5 cut(s) 12, 935, 2853, 3408, 3918
MvnI CGCG 2 cut(s) 2082, 3391
NciI CCSGG 2 cut(s) 509, 3498
NcoI CCATGG 2 cut(s) 468, 3195
NdeI CATATG 1 cut(s) 2985
NheI GCTAGC 1 cut(s) 3089
NlaIV GGNNCC 5 cut(s) 273, 352, 1951, 2837, 3547
NmuCI GTSAC 7 cut(s) 245, 1751, 2161, 2759, 3371, 3521, 4213
NsiI ATGCAT 5 cut(s) 6, 370, 2566, 3802, 4536
NspI RCATGY 4 cut(s) 1884, 2452, 3800, 4123
PaeI GCATGC 1 cut(s) 1884
PaeR7I CTCGAG 1 cut(s) 1052
PagI TCATGA 2 cut(s) 540, 1269
PciI ACATGT 2 cut(s) 2448, 4119
PctI GAATGC 4 cut(s) 458, 1078, 2076, 4778
PdmI GAANNNNTTC 3 cut(s) 1176, 1376, 4317
PflMI CCANNNNNTGG 2 cut(s) 1867, 2030
PfoI TCCNGGA 2 cut(s) 3406, 3916
Ple19I CGATCG 1 cut(s) 4378
PleI GAGTC 5 cut(s) 880, 3748, 4038, 4210, 4808
PpsI GAGTC 5 cut(s) 880, 3748, 4038, 4210, 4808
Ppu21I YACGTR 1 cut(s) 3371
PpuMI RGGWCCY 1 cut(s) 4643
PscI ACATGT 2 cut(s) 2448, 4119
PshAI GACNNNNGTC 1 cut(s) 901
PshBI ATTAAT 1 cut(s) 2183
PsiI TTATAA 3 cut(s) 753, 1541, 4616
Psp1406I AACGTT 1 cut(s) 4742
Psp5II RGGWCCY 1 cut(s) 4643
Psp6I CCWGG 5 cut(s) 10, 933, 2851, 3406, 3916
PspFI CCCAGC 1 cut(s) 2383
PspGI CCWGG 5 cut(s) 10, 933, 2851, 3406, 3916
PspN4I GGNNCC 5 cut(s) 273, 352, 1951, 2837, 3547
PspPI GGNCC 9 cut(s) 92, 271, 1874, 2021, 2624, 3404, 3868, 4100, 4643
PspPPI RGGWCCY 1 cut(s) 4643
PstI CTGCAG 1 cut(s) 4822
PstNI CAGNNNCTG 3 cut(s) 2030, 2948, 4574
PsuI RGATCY 5 cut(s) 921, 1210, 1633, 2835, 4783
PvuI CGATCG 1 cut(s) 4378
PvuII CAGCTG 2 cut(s) 2387, 4342
RseI CAYNNNNRTG 4 cut(s) 1229, 1541, 2586, 3175
Sau96I GGNCC 9 cut(s) 92, 271, 1874, 2021, 2624, 3404, 3868, 4100, 4643
ScaI AGTACT 2 cut(s) 1775, 2816
SchI GAGTC 5 cut(s) 880, 3749, 4039, 4210, 4808
ScrFI CCNGG 7 cut(s) 12, 509, 935, 2853, 3408, 3498, 3918
SduI GDGCHC 4 cut(s) 1325, 1516, 2578, 2780
SfcI CTRYAG 2 cut(s) 3240, 4818
Sfr274I CTCGAG 1 cut(s) 1052
SinI GGWCC 5 cut(s) 92, 1874, 3404, 3868, 4643
SlaI CTCGAG 1 cut(s) 1052
SmiMI CAYNNNNRTG 4 cut(s) 1229, 1541, 2586, 3175
SmlI CTYRAG 8 cut(s) 448, 1052, 2411, 3656, 3737, 3969, 4229, 4430
SmoI CTYRAG 8 cut(s) 448, 1052, 2411, 3656, 3737, 3969, 4229, 4430
SphI GCATGC 1 cut(s) 1884
SsiI CCGC 7 cut(s) 892, 1853, 2036, 2869, 3389, 3444, 4392
SspI AATATT 1 cut(s) 2227
StyD4I CCNGG 7 cut(s) 10, 507, 933, 2851, 3406, 3496, 3916
StyI CCWWGG 9 cut(s) 354, 468, 943, 1784, 1866, 3195, 3666, 3768, 4787
TaiI ACGT 4 cut(s) 1465, 2974, 3373, 4745
TaqI TCGA 9 cut(s) 809, 1053, 2374, 3067, 3349, 3912, 4374, 4378, 4845
TatI WGTACW 8 cut(s) 1495, 1661, 1773, 2796, 2814, 3008, 4336, 4746
TauI GCSGC 2 cut(s) 1856, 2872
TseFI GTSAC 7 cut(s) 245, 1751, 2161, 2759, 3371, 3521, 4213
Tsp45I GTSAC 7 cut(s) 245, 1751, 2161, 2759, 3371, 3521, 4213
TspGWI ACGGA 4 cut(s) 1427, 1721, 2216, 3390
Van91I CCANNNNNTGG 2 cut(s) 1867, 2030
Vha464I CTTAAG 2 cut(s) 448, 2411
VpaK11BI GGWCC 5 cut(s) 92, 1874, 3404, 3868, 4643
VspI ATTAAT 1 cut(s) 2183
XapI RAATTY 7 cut(s) 395, 548, 1101, 1431, 2138, 2336, 2402
XbaI TCTAGA 3 cut(s) 982, 1502, 3154
XceI RCATGY 4 cut(s) 1884, 2452, 3800, 4123
XcmI CCANNNNNNNNNTGG 3 cut(s) 331, 1868, 3202
XhoI CTCGAG 1 cut(s) 1052
XmaJI CCTAGG 1 cut(s) 4787
XmiI GTMKAC 2 cut(s) 3236, 4188
XmnI GAANNNNTTC 3 cut(s) 1176, 1376, 4317
ZrmI AGTACT 2 cut(s) 1775, 2816
Zsp2I ATGCAT 5 cut(s) 6, 370, 2566, 3802, 4536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.