AT3G49601

cwf21 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002497
Physical Location & Seq
Reverse (-)
18387267 .. 18389571
2305 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G49601.1

Sequence Viewer

Length: 1776 bp
ATGTACAACGGAATAGGGTTACAGACAGCAAGAGGATCAGGAACTAATGGTTATGTTCAGACGAATAAGTTTTTTGTGAGACCTAGAAATGGTGGTAAGCCTGTTAAAGGTGGGAAAGGTTTTGAGGATGATGAAGGTACTGCTGGTTTATCTAAGAAACCCAATAAAGCTATCCTTGAGCATGACCGTAAACGTCAGATTCATCTTAAGCTTGCTATTCTTGAGGATAAGCTTGCTGATCAGGGTTATTCTGATATTGAGATTGCTCAGAAGCTTGAGGAAGCTAGAGTGAGTCTTGAAGCTGCTGCTGCTGCTAATGAAGAAGAGAGTGATTCGAAGGTTTCTAATACTCAGACTCATCAGGTTGCTGCTAGGAAAGAGAAGCAGATGGAAGCGTTTCGAGCTGCGTTGGGTTTGCCTGATCAGCAACAGGTGGCTGAGGAGGGTATTATTGATGATGAGCCGATGAGGGAGGGTTTTGAAGGTAGATTGAAAGAGAGGCGGGAACATTCTTTTTTGGACAGAGATAGTGGGAGGAAGAAGGTAGATGAGGATGTGGATGAGAAAGATGCTAAGGTAAAGGAAAGCAAGAAGCAGCGAGGTGGTGATGATGATGATGTGGATGTAGTGAAGCGTCATAAGAAAAAAGAGAGCAAGAAGAGAAGACATGATGATTCATCTGAATCTGATGAGCATGGTCGTGACCGTAGAAGGCGCTCTAAGAAGAAGGCTAAGGGGCGTAAACAAGAAAGTGAAAGTGACTCTAGCAGCTCGGATTCTGAATCTGACTCTGACAGTGATGATGGAAAGAAGAGAGGAAGGAAGAAGCCTACAAAGACTACAAAGAAACGCAGCAGAAGGAAAAGAAGTGTCTCTTCTGAATCTGAAGAAGTTGAGAGTGATGATAGCAAGAAACTTAGGAAGTCACACAAAAAGAGCCTCCCCTCCAACCGATCTGGTTCCAAAGAATTAAGGGACAAACATGATGAACAAAGCCGAGCTGGACGAAAGAGGCATGATTCTGATGTTAGCGAGCCTGAATCGGAGGATAATAAACAACCACTGCGAAAGAAAGAAGAAGCTTACCGTGGAGGACAGAAGCAGAAAAGAGATGATGAAGACGTGGAGGCTGATCATTTGAAAGACAGATATACTAGAGATGACAAGAAAGCTGCAAGAGACTCTGATGACAGTGAGATTGAGTATCAGAACAAGAAACAACTGCGCAGTAAGGTAGAAGTTTACAGTGCAGGAATGAGCCAGAAAAGAAAAGAGGAAGAAGATGTGACCAAACATGGCAAGGACAAGTATAGAAGTGATTCTCGTGGCAAGGAAGTTGCTAGAGACTCTGATGACAGTGAGGCTGAGTATGAAAATAGGAAGAAGCTAAAGAATGAAAGTTACCAACGAGGACGTAAACACAAAAGAGAGGAGGATGAGGACAACGATAACCATGGGAGGGACAGATATAGAGGTGATGATGCTGTTAAAAGATATGGAACAATTAAGGAAGACGACGATAGGTATAGAGGTCGAGCCATTGAGGAAGAAGGAGATGATGATAGGGGTAGATACAGACCGAGACGAGAAAGTGTGAAGGATGATGAGGAAGAGTATAAGCATGGGAGAGACAGGTACAGAGGTGATGGAAGACGAGCAACGGGTAAGGAAGATGATGATGATGATAGAGTAAGCAGAGAGCGTGAATACTCAAGCAGGGGTCGAAGTCGTTATGATGATAGCCGATCAAGTGGCAAGAGATCCTCGCATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cwf21

Protein Analysis

591

Amino Acids

68.35

Weight (kDa)

8.96

Isoelectric Point (pI)

61.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf21 PF08312 59 - 101 6.7e-13 cwf21 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1226
AciI CCGC 1 cut(s) 502
AclWI GGATC 2 cut(s) 43, 1755
AcuI CTGAAG 1 cut(s) 906
AfaI GTAC 3 cut(s) 5, 139, 1637
AfiI CCNNNNNNNGG 3 cut(s) 89, 107, 1459
AflII CTTAAG 1 cut(s) 206
AgsI TTSAA 4 cut(s) 299, 482, 493, 1141
AjiI CACGTC 1 cut(s) 1123
Alw26I GTCTC 6 cut(s) 73, 877, 1173, 1338, 1576, 1623
AlwI GGATC 2 cut(s) 43, 1755
Asp700I GAANNNNTTC 2 cut(s) 396, 1318
AspLEI GCGC 2 cut(s) 717, 1227
AsuHPI GGTGA 3 cut(s) 617, 1487, 1655
AsuII TTCGAA 1 cut(s) 335
BaeI ACNNNNGTAYC 2 cut(s) 1627, 1660
BauI CACGAG 1 cut(s) 1323
BbsI GAAGAC 4 cut(s) 670, 1125, 1518, 1657
BbvCI CCTCAGC 1 cut(s) 438
BccI CCATC 3 cut(s) 382, 797, 1640
BclI TGATCA 3 cut(s) 238, 421, 1132
BcoDI GTCTC 6 cut(s) 73, 877, 1173, 1338, 1576, 1623
BfaI CTAG 6 cut(s) 84, 285, 372, 765, 1155, 1341
BfoI RGCGCY 1 cut(s) 718
BfrI CTTAAG 1 cut(s) 206
BmgBI CACGTC 1 cut(s) 1123
BmiI GGNNCC 1 cut(s) 961
BmsI GCATC 2 cut(s) 559, 1471
BpiI GAAGAC 4 cut(s) 670, 1125, 1518, 1657
Bpu10I CCTNAGC 3 cut(s) 438, 573, 732
Bpu14I TTCGAA 1 cut(s) 335
BpuEI CTTGAG 4 cut(s) 197, 242, 296, 1696
BsaI GGTCTC 1 cut(s) 73
BsaJI CCNNGG 2 cut(s) 1087, 1453
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 107, 1459
BseDI CCNNGG 2 cut(s) 1087, 1453
BseGI GGATG 6 cut(s) 133, 559, 565, 628, 1441, 1606
BseLI CCNNNNNNNGG 3 cut(s) 89, 107, 1459
BseMII CTCAG 4 cut(s) 281, 365, 429, 1356
BseRI GAGGAG 2 cut(s) 455, 1445
BsgI GTGCAG 1 cut(s) 1269
BslFI GGGAC 2 cut(s) 989, 1475
BslI CCNNNNNNNGG 3 cut(s) 89, 107, 1459
BsmAI GTCTC 6 cut(s) 73, 877, 1173, 1338, 1576, 1623
BsmBI CGTCTC 1 cut(s) 1576
BsmFI GGGAC 2 cut(s) 989, 1475
Bso31I GGTCTC 1 cut(s) 73
Bsp119I TTCGAA 1 cut(s) 335
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 7 cut(s) 35, 238, 421, 953, 1132, 1745, 1760
Bsp19I CCATGG 1 cut(s) 1453
BspACI CCGC 1 cut(s) 502
BspCNI CTCAG 4 cut(s) 280, 364, 430, 1357
BspLI GGNNCC 1 cut(s) 961
BspPI GGATC 2 cut(s) 43, 1755
BspT104I TTCGAA 1 cut(s) 335
BspTI CTTAAG 1 cut(s) 206
BspTNI GGTCTC 1 cut(s) 73
BsrGI TGTACA 1 cut(s) 3
BssECI CCNNGG 2 cut(s) 1087, 1453
BssMI GATC 7 cut(s) 35, 238, 421, 953, 1132, 1745, 1760
BssSI CACGAG 1 cut(s) 1323
BssT1I CCWWGG 1 cut(s) 1453
Bst2BI CACGAG 1 cut(s) 1323
Bst4CI ACNGT 7 cut(s) 188, 707, 797, 1088, 1193, 1247, 1358
Bst6I CTCTTC 5 cut(s) 318, 653, 806, 880, 1605
BstAFI CTTAAG 1 cut(s) 206
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 335
BstC8I GCNNGC 3 cut(s) 213, 234, 1034
BstDEI CTNAG 9 cut(s) 153, 267, 351, 438, 573, 720, 732, 917, 1365
BstDSI CCRYGG 2 cut(s) 1087, 1453
BstENI CCTNNNNNAGG 1 cut(s) 105
BstF5I GGATG 6 cut(s) 133, 559, 565, 628, 1441, 1606
BstH2I RGCGCY 1 cut(s) 718
BstHHI GCGC 2 cut(s) 717, 1227
BstKTI GATC 7 cut(s) 38, 241, 424, 956, 1135, 1748, 1763
BstMAI GTCTC 6 cut(s) 73, 877, 1173, 1338, 1576, 1623
BstMBI GATC 7 cut(s) 35, 238, 421, 953, 1132, 1745, 1760
BstMWI GCNNNNNNNGC 4 cut(s) 308, 311, 401, 424
BstV2I GAAGAC 4 cut(s) 670, 1125, 1518, 1657
BstX2I RGATCY 1 cut(s) 1760
BstYI RGATCY 1 cut(s) 1760
BtgI CCRYGG 2 cut(s) 1087, 1453
BtrI CACGTC 1 cut(s) 1123
BtsCI GGATG 6 cut(s) 133, 559, 565, 628, 1441, 1606
BtsI GCAGTG 1 cut(s) 1061
BtsIMutI CAGTG 5 cut(s) 802, 1061, 1198, 1252, 1363
Cac8I GCNNGC 3 cut(s) 213, 234, 1034
CfoI GCGC 2 cut(s) 717, 1227
CseI GACGC 1 cut(s) 623
Csp6I GTAC 3 cut(s) 4, 138, 1636
CviAII CATG 9 cut(s) 182, 668, 695, 983, 1016, 1295, 1454, 1622, 1769
CviQI GTAC 3 cut(s) 4, 138, 1636
DdeI CTNAG 9 cut(s) 153, 267, 351, 438, 573, 720, 732, 917, 1365
DpnI GATC 7 cut(s) 37, 240, 423, 955, 1134, 1747, 1762
DpnII GATC 7 cut(s) 35, 238, 421, 953, 1132, 1745, 1760
Eam1104I CTCTTC 5 cut(s) 318, 653, 806, 880, 1605
EarI CTCTTC 5 cut(s) 318, 653, 806, 880, 1605
Eco130I CCWWGG 1 cut(s) 1453
Eco31I GGTCTC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 906
EcoNI CCTNNNNNAGG 1 cut(s) 105
EcoT14I CCWWGG 1 cut(s) 1453
ErhI CCWWGG 1 cut(s) 1453
Esp3I CGTCTC 1 cut(s) 1576
FaeI CATG 9 cut(s) 185, 671, 698, 986, 1019, 1298, 1457, 1625, 1772
FalI AAGNNNNNCTT 4 cut(s) 159, 191, 859, 891
FaqI GGGAC 2 cut(s) 989, 1475
FatI CATG 9 cut(s) 181, 667, 694, 982, 1015, 1294, 1453, 1621, 1768
FauI CCCGC 1 cut(s) 495
FbaI TGATCA 3 cut(s) 238, 421, 1132
FokI GGATG 6 cut(s) 140, 566, 572, 635, 1448, 1613
FspBI CTAG 6 cut(s) 84, 285, 372, 765, 1155, 1341
FspI TGCGCA 1 cut(s) 1226
GlaI GCGC 2 cut(s) 716, 1226
HaeII RGCGCY 1 cut(s) 718
HgaI GACGC 1 cut(s) 623
HhaI GCGC 2 cut(s) 717, 1227
Hin1II CATG 9 cut(s) 185, 671, 698, 986, 1019, 1298, 1457, 1625, 1772
Hin6I GCGC 2 cut(s) 715, 1225
HinP1I GCGC 2 cut(s) 715, 1225
HindIII AAGCTT 4 cut(s) 209, 230, 272, 1080
HphI GGTGA 3 cut(s) 617, 1487, 1655
Hpy166II GTNNAC 4 cut(s) 191, 743, 1243, 1418
Hpy188III TCNNGA 4 cut(s) 39, 221, 296, 701
Hpy8I GTNNAC 4 cut(s) 191, 743, 1243, 1418
Hpy99I CGWCG 1 cut(s) 1520
HpyCH4III ACNGT 7 cut(s) 188, 707, 797, 1088, 1193, 1247, 1358
HpyCH4IV ACGT 3 cut(s) 193, 1122, 1414
HpyCH4V TGCA 2 cut(s) 1175, 1250
HpyF10VI GCNNNNNNNGC 4 cut(s) 308, 311, 401, 424
HpyF3I CTNAG 9 cut(s) 153, 267, 351, 438, 573, 720, 732, 917, 1365
HpySE526I ACGT 3 cut(s) 193, 1122, 1414
Hsp92II CATG 9 cut(s) 185, 671, 698, 986, 1019, 1298, 1457, 1625, 1772
HspAI GCGC 2 cut(s) 715, 1225
Ksp22I TGATCA 3 cut(s) 238, 421, 1132
Kzo9I GATC 7 cut(s) 35, 238, 421, 953, 1132, 1745, 1760
LweI GCATC 2 cut(s) 559, 1471
MaeI CTAG 6 cut(s) 84, 285, 372, 765, 1155, 1341
MaeII ACGT 3 cut(s) 193, 1122, 1414
MaeIII GTNAC 6 cut(s) 18, 701, 758, 924, 1285, 1400
MalI GATC 7 cut(s) 37, 240, 423, 955, 1134, 1747, 1762
MboI GATC 7 cut(s) 35, 238, 421, 953, 1132, 1745, 1760
MflI RGATCY 1 cut(s) 1760
MluCI AATT 2 cut(s) 968, 1503
MlyI GAGTC 6 cut(s) 301, 349, 755, 782, 1175, 1340
MmeI TCCRAC 1 cut(s) 972
MroXI GAANNNNTTC 2 cut(s) 396, 1318
MseI TTAA 5 cut(s) 105, 207, 971, 1488, 1506
MslI CAYNNNNRTG 1 cut(s) 699
MspCI CTTAAG 1 cut(s) 206
MwoI GCNNNNNNNGC 4 cut(s) 308, 311, 401, 424
NcoI CCATGG 1 cut(s) 1453
NdeII GATC 7 cut(s) 35, 238, 421, 953, 1132, 1745, 1760
NlaIII CATG 9 cut(s) 185, 671, 698, 986, 1019, 1298, 1457, 1625, 1772
NlaIV GGNNCC 1 cut(s) 961
NmeAIII GCCGAG 1 cut(s) 1022
NmuCI GTSAC 4 cut(s) 701, 758, 924, 1285
NsbI TGCGCA 1 cut(s) 1226
NspV TTCGAA 1 cut(s) 335
PdmI GAANNNNTTC 2 cut(s) 396, 1318
PleI GAGTC 6 cut(s) 300, 349, 755, 782, 1175, 1340
PpsI GAGTC 6 cut(s) 300, 349, 755, 782, 1175, 1340
PspN4I GGNNCC 1 cut(s) 961
PsuI RGATCY 1 cut(s) 1760
RsaI GTAC 3 cut(s) 5, 139, 1637
RsaNI GTAC 3 cut(s) 4, 138, 1636
RseI CAYNNNNRTG 1 cut(s) 699
SaqAI TTAA 5 cut(s) 105, 207, 971, 1488, 1506
Sau3AI GATC 7 cut(s) 35, 238, 421, 953, 1132, 1745, 1760
SchI GAGTC 6 cut(s) 301, 349, 755, 782, 1175, 1340
SfaNI GCATC 2 cut(s) 559, 1471
SfuI TTCGAA 1 cut(s) 335
SmiMI CAYNNNNRTG 1 cut(s) 699
SmlI CTYRAG 5 cut(s) 176, 206, 221, 275, 1711
SmoI CTYRAG 5 cut(s) 176, 206, 221, 275, 1711
Sse9I AATT 2 cut(s) 968, 1503
SsiI CCGC 1 cut(s) 502
SspMI CTAG 6 cut(s) 84, 285, 372, 765, 1155, 1341
StyI CCWWGG 1 cut(s) 1453
TaaI ACNGT 7 cut(s) 188, 707, 797, 1088, 1193, 1247, 1358
TaiI ACGT 3 cut(s) 196, 1125, 1417
TaqI TCGA 4 cut(s) 335, 400, 1534, 1723
TaqII GACCGA 1 cut(s) 1594
TasI AATT 2 cut(s) 968, 1503
TatI WGTACW 1 cut(s) 3
Tru1I TTAA 5 cut(s) 105, 207, 971, 1488, 1506
Tru9I TTAA 5 cut(s) 105, 207, 971, 1488, 1506
TscAI CASTG 5 cut(s) 802, 1068, 1198, 1252, 1363
TseFI GTSAC 4 cut(s) 701, 758, 924, 1285
Tsp45I GTSAC 4 cut(s) 701, 758, 924, 1285
TspDTI ATGAA 8 cut(s) 147, 191, 333, 666, 1002, 1131, 1386, 1410
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 5 cut(s) 802, 1068, 1198, 1252, 1363
Vha464I CTTAAG 1 cut(s) 206
XagI CCTNNNNNAGG 1 cut(s) 105
XmnI GAANNNNTTC 2 cut(s) 396, 1318
XspI CTAG 6 cut(s) 84, 285, 372, 765, 1155, 1341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.