RLG00000029792

cwf21 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002497
Physical Location & Seq
Forward (+)
49168824 .. 49169826
1003 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029792

Sequence Viewer

Length: 720 bp
ATGGGTGCAGTGTGTGCAATTTTCTTTGACTCCGTTTCAAGGATGGGGAGATTTTTCACGTTCCTCATTCACGCTCTAGAAAACCGGGCATCCAGTGTACGCGCAGTATTTAACCGTGGTGCAGAGGCCATTGGGACTTTGGAAGAATTGCCAAAGAGCATATCTGAAAAGGGCAAACAACATGTGACACCAAACAGAAGGCATGACTCTGGGGATGAGTCTGACTTTGACAGCAGAATGGAAAGGAGAAGTAGCCAAGTTGAGCAGCAAAGGAATCAGCAGGTCAGCCACAGGGTAGATCGAGATGATTTTGATGTGGAGAAGGCCGATAATGTTAGAAGTGGAAAAAGCATAAAATCGTATGATAGTGATTCTTTTTCTGACTATGAAAGATCCAGAAAAAGTAGAGGTGCAATAACAAAAAAAAGTAGAAGAAGTGGCGGGAATGATACTAGTGATGATGATACTAATATTGGGAGGAAACCTGCAAGGATTGAAGAGGGTGATGGAGGACGTGGAAGGCACAACAAGAGTGAACTGCAAAGAGGAAGGAAAAATGAAAGAGATGAAGAGGATCATGATTATAGGAGGTCCAGGGAAGGTCTGGAACAGGAACTTGGAAATAGAGAGCGTGGTACTAGAGAGAAAGAAGGGGCAAATGAGGATATTGAAAGGGATAGACATATGGATTACAGGAGAGCAAAATATGACGGAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cwf21

Protein Analysis

240

Amino Acids

27.55

Weight (kDa)

7.88

Isoelectric Point (pI)

61.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 271, 493
AccII CGCG 1 cut(s) 102
AciI CCGC 1 cut(s) 441
AclWI GGATC 2 cut(s) 387, 582
AfaI GTAC 2 cut(s) 99, 637
AfiI CCNNNNNNNGG 1 cut(s) 39
AflIII ACRYGT 1 cut(s) 181
AgsI TTSAA 3 cut(s) 39, 497, 671
AhlI ACTAGT 1 cut(s) 452
AjiI CACGTC 1 cut(s) 515
AjnI CCWGG 1 cut(s) 593
AjuI GAANNNNNNNTTGG 2 cut(s) 600, 632
AlwI GGATC 2 cut(s) 387, 582
AoxI GGCC 2 cut(s) 126, 324
ApeKI GCWGC 1 cut(s) 265
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 1 cut(s) 591
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 1 cut(s) 515
AvaII GGWCC 1 cut(s) 591
BbvI GCAGC 1 cut(s) 277
BccI CCATC 2 cut(s) 37, 500
BciT130I CCWGG 1 cut(s) 595
BcnI CCSGG 1 cut(s) 86
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 4 cut(s) 77, 453, 639, 718
BfuAI ACCTGC 2 cut(s) 271, 493
BisI GCNGC 1 cut(s) 266
BlsI GCNGC 1 cut(s) 267
Bme1390I CCNGG 2 cut(s) 86, 595
Bme18I GGWCC 1 cut(s) 591
BmgBI CACGTC 1 cut(s) 515
BmgT120I GGNCC 1 cut(s) 591
BmrFI CCNGG 2 cut(s) 86, 595
BmsI GCATC 1 cut(s) 98
BpuMI CCSGG 1 cut(s) 86
BsaJI CCNNGG 2 cut(s) 115, 594
Bsc4I CCNNNNNNNGG 1 cut(s) 39
Bse1I ACTGG 1 cut(s) 93
BseBI CCWGG 1 cut(s) 595
BseDI CCNNGG 2 cut(s) 115, 594
BseGI GGATG 3 cut(s) 48, 89, 220
BseLI CCNNNNNNNGG 1 cut(s) 39
BseNI ACTGG 1 cut(s) 93
BseXI GCAGC 1 cut(s) 277
BsgI GTGCAG 2 cut(s) 27, 141
Bsh1236I CGCG 1 cut(s) 102
BshFI GGCC 2 cut(s) 128, 326
BsiSI CCGG 1 cut(s) 85
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 1 cut(s) 39
BsmFI GGGAC 1 cut(s) 148
BsnI GGCC 2 cut(s) 128, 326
Bsp143I GATC 3 cut(s) 298, 392, 574
BspACI CCGC 1 cut(s) 441
BspANI GGCC 2 cut(s) 128, 326
BspFNI CGCG 1 cut(s) 102
BspHI TCATGA 1 cut(s) 577
BspMI ACCTGC 2 cut(s) 271, 493
BspPI GGATC 2 cut(s) 387, 582
BsrI ACTGG 1 cut(s) 93
BssECI CCNNGG 2 cut(s) 115, 594
BssMI GATC 3 cut(s) 298, 392, 574
Bst2UI CCWGG 1 cut(s) 595
Bst4CI ACNGT 1 cut(s) 116
Bst6I CTCTTC 2 cut(s) 492, 564
BstAPI GCANNNNNTGC 1 cut(s) 14
BstDSI CCRYGG 1 cut(s) 115
BstF5I GGATG 3 cut(s) 48, 89, 220
BstFNI CGCG 1 cut(s) 102
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 3 cut(s) 301, 395, 577
BstMBI GATC 3 cut(s) 298, 392, 574
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNI CCWGG 1 cut(s) 595
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 2 cut(s) 84, 593
BstUI CGCG 1 cut(s) 102
BstV1I GCAGC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 392
BstYI RGATCY 1 cut(s) 392
BsuRI GGCC 2 cut(s) 128, 326
BtgI CCRYGG 1 cut(s) 115
BtrI CACGTC 1 cut(s) 515
BtsCI GGATG 3 cut(s) 48, 89, 220
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 2 cut(s) 15, 100
BveI ACCTGC 2 cut(s) 271, 493
CciI TCATGA 1 cut(s) 577
CfoI GCGC 1 cut(s) 104
Cfr13I GGNCC 1 cut(s) 591
Csp6I GTAC 2 cut(s) 98, 636
CviAII CATG 3 cut(s) 182, 203, 578
CviJI RGCY 4 cut(s) 128, 255, 288, 326
CviKI_1 RGCY 4 cut(s) 128, 255, 288, 326
CviQI GTAC 2 cut(s) 98, 636
DpnI GATC 3 cut(s) 300, 394, 576
DpnII GATC 3 cut(s) 298, 392, 574
Eam1104I CTCTTC 2 cut(s) 492, 564
EarI CTCTTC 2 cut(s) 492, 564
Eco47I GGWCC 1 cut(s) 591
EcoRII CCWGG 1 cut(s) 593
FaeI CATG 3 cut(s) 185, 206, 581
FaqI GGGAC 1 cut(s) 148
FatI CATG 3 cut(s) 181, 202, 577
FauI CCCGC 1 cut(s) 434
FauNDI CATATG 1 cut(s) 684
Fnu4HI GCNGC 1 cut(s) 266
FokI GGATG 3 cut(s) 55, 76, 227
Fsp4HI GCNGC 1 cut(s) 266
FspBI CTAG 4 cut(s) 77, 453, 639, 718
GlaI GCGC 1 cut(s) 103
GluI GCNGC 1 cut(s) 266
HaeIII GGCC 2 cut(s) 128, 326
HapII CCGG 1 cut(s) 85
HhaI GCGC 1 cut(s) 104
Hin1II CATG 3 cut(s) 185, 206, 581
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HinfI GANTC 6 cut(s) 29, 206, 218, 274, 371, 714
HpaII CCGG 1 cut(s) 85
HphI GGTGA 1 cut(s) 515
Hpy166II GTNNAC 2 cut(s) 98, 536
Hpy188I TCNGA 3 cut(s) 166, 223, 382
Hpy188III TCNNGA 5 cut(s) 77, 302, 396, 578, 605
Hpy8I GTNNAC 2 cut(s) 98, 536
HpyAV CCTTC 6 cut(s) 192, 316, 513, 543, 593, 644
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 2 cut(s) 59, 514
HpyCH4V TGCA 6 cut(s) 8, 17, 122, 413, 488, 541
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
HpySE526I ACGT 2 cut(s) 59, 514
Hsp92II CATG 3 cut(s) 185, 206, 581
HspAI GCGC 1 cut(s) 102
Kzo9I GATC 3 cut(s) 298, 392, 574
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 1 cut(s) 98
MaeI CTAG 4 cut(s) 77, 453, 639, 718
MaeII ACGT 2 cut(s) 59, 514
MaeIII GTNAC 1 cut(s) 184
MalI GATC 3 cut(s) 300, 394, 576
MboI GATC 3 cut(s) 298, 392, 574
MboII GAAGA 4 cut(s) 155, 444, 509, 581
MflI RGATCY 1 cut(s) 392
MluCI AATT 2 cut(s) 18, 146
MlyI GAGTC 3 cut(s) 23, 200, 227
MseI TTAA 1 cut(s) 111
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 2 cut(s) 86, 595
MvaI CCWGG 1 cut(s) 595
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 1 cut(s) 14
NciI CCSGG 1 cut(s) 86
NdeI CATATG 1 cut(s) 684
NdeII GATC 3 cut(s) 298, 392, 574
NlaIII CATG 3 cut(s) 185, 206, 581
NmuCI GTSAC 1 cut(s) 184
NspI RCATGY 1 cut(s) 185
PagI TCATGA 1 cut(s) 577
PciI ACATGT 1 cut(s) 181
PfeI GAWTC 2 cut(s) 274, 371
PflFI GACNNNGTC 1 cut(s) 713
PkrI GCNGC 1 cut(s) 267
PleI GAGTC 3 cut(s) 23, 200, 226
PpsI GAGTC 3 cut(s) 23, 200, 226
PscI ACATGT 1 cut(s) 181
Psp6I CCWGG 1 cut(s) 593
PspGI CCWGG 1 cut(s) 593
PspPI GGNCC 1 cut(s) 591
PsuI RGATCY 1 cut(s) 392
PsyI GACNNNGTC 1 cut(s) 713
RsaI GTAC 2 cut(s) 99, 637
RsaNI GTAC 2 cut(s) 98, 636
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 1 cut(s) 266
Sau3AI GATC 3 cut(s) 298, 392, 574
Sau96I GGNCC 1 cut(s) 591
SchI GAGTC 3 cut(s) 23, 200, 227
ScrFI CCNGG 2 cut(s) 86, 595
SetI ASST 7 cut(s) 62, 285, 412, 487, 517, 593, 604
SfaNI GCATC 1 cut(s) 98
SinI GGWCC 1 cut(s) 591
SpeI ACTAGT 1 cut(s) 452
Sse9I AATT 2 cut(s) 18, 146
SsiI CCGC 1 cut(s) 441
SspI AATATT 1 cut(s) 472
SspMI CTAG 4 cut(s) 77, 453, 639, 718
StyD4I CCNGG 2 cut(s) 84, 593
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 2 cut(s) 62, 517
TaqI TCGA 1 cut(s) 301
TasI AATT 2 cut(s) 18, 146
TfiI GAWTC 2 cut(s) 274, 371
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 2 cut(s) 15, 100
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 1 cut(s) 265
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 3 cut(s) 402, 573, 582
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 2 cut(s) 15, 100
Tth111I GACNNNGTC 1 cut(s) 713
VpaK11BI GGWCC 1 cut(s) 591
XbaI TCTAGA 1 cut(s) 76
XceI RCATGY 1 cut(s) 185
XcmI CCANNNNNNNNNTGG 2 cut(s) 136, 601
XspI CTAG 4 cut(s) 77, 453, 639, 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.