Rh4BG159600

cwf21 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002497
Physical Location & Seq
Forward (+)
28793950 .. 28794309
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG159600.1

Sequence Viewer

Length: 360 bp
ATGTACAACGGAATAGGGTTACAGACTCCGAGAGGGTCTGGAACCAATGGCAATATCCAGACGAACAAGTTCTTCATCAGGTCCAAGACTGGCAAGGTTGCTGAAAACTCCAGAGGGTTTGAGGGAGACCAGGGCATGGCTGGCGTCACAAAGAAGACCAACAGAGACATCCTTGAGCATGATCGCAAGCGCCAGATTGAGCTCAAGCTTGTGGTGCTGGAGGAGAATCTGAGCGATCAGGGATTCACGGAGGCTGAGATTGGTGAGAAGGTCACCGAGGCTCAGAGGACTTTGGAAGCTGCTGCCGCGTCTGAGGAGATAGGTGGAACCACTGCTATTGTTCTTGGAGATAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cwf21

Protein Analysis

119

Amino Acids

12.91

Weight (kDa)

5.9

Isoelectric Point (pI)

30.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf21 PF08312 58 - 100 7.1e-14 cwf21 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 136
AccII CGCG 1 cut(s) 308
AciI CCGC 1 cut(s) 306
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 136
AjnI CCWGG 1 cut(s) 129
AluBI AGCT 3 cut(s) 202, 208, 299
AluI AGCT 3 cut(s) 202, 208, 299
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 120, 159
ApeKI GCWGC 2 cut(s) 299, 302
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 265, 275
AvaII GGWCC 1 cut(s) 81
BanII GRGCYC 1 cut(s) 204
BbsI GAAGAC 1 cut(s) 161
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 286, 289
BciT130I CCWGG 1 cut(s) 131
BcoDI GTCTC 2 cut(s) 120, 159
BfoI RGCGCY 1 cut(s) 193
BisI GCNGC 3 cut(s) 300, 303, 306
BlsI GCNGC 3 cut(s) 301, 304, 307
Bme1390I CCNGG 1 cut(s) 131
Bme18I GGWCC 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 81
BmiI GGNNCC 2 cut(s) 43, 328
BmrFI CCNGG 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 161
BpmI CTGGAG 2 cut(s) 94, 239
BpuEI CTTGAG 2 cut(s) 188, 194
BsaHI GRCGYC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 130, 276
Bsc4I CCNNNNNNNGG 1 cut(s) 136
Bse1I ACTGG 1 cut(s) 94
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 2 cut(s) 130, 276
BseGI GGATG 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 136
BseMII CTCAG 4 cut(s) 221, 246, 296, 303
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 2 cut(s) 236, 329
BseXI GCAGC 2 cut(s) 286, 289
Bsh1236I CGCG 1 cut(s) 308
BsiHKAI GWGCWC 1 cut(s) 204
BslI CCNNNNNNNGG 1 cut(s) 136
BsmAI GTCTC 2 cut(s) 120, 159
Bso31I GGTCTC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 181, 235
BspACI CCGC 1 cut(s) 306
BspCNI CTCAG 4 cut(s) 222, 247, 295, 304
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 2 cut(s) 43, 328
BspTNI GGTCTC 1 cut(s) 120
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 94
BssECI CCNNGG 2 cut(s) 130, 276
BssMI GATC 2 cut(s) 181, 235
BssNI GRCGYC 1 cut(s) 144
Bst2UI CCWGG 1 cut(s) 131
BstACI GRCGYC 1 cut(s) 144
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 142, 188
BstDEI CTNAG 4 cut(s) 230, 255, 282, 312
BstEII GGTNACC 1 cut(s) 271
BstF5I GGATG 1 cut(s) 168
BstFNI CGCG 1 cut(s) 308
BstH2I RGCGCY 1 cut(s) 193
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 2 cut(s) 184, 238
BstMAI GTCTC 2 cut(s) 120, 159
BstMBI GATC 2 cut(s) 181, 235
BstMWI GCNNNNNNNGC 3 cut(s) 141, 214, 305
BstNI CCWGG 1 cut(s) 131
BstPI GGTNACC 1 cut(s) 271
BstSCI CCNGG 1 cut(s) 129
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 2 cut(s) 286, 289
BstV2I GAAGAC 1 cut(s) 161
BtsCI GGATG 1 cut(s) 168
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 1 cut(s) 330
Cac8I GCNNGC 2 cut(s) 142, 188
CfoI GCGC 1 cut(s) 192
Cfr13I GGNCC 1 cut(s) 81
CseI GACGC 2 cut(s) 133, 297
Csp6I GTAC 1 cut(s) 4
CspCI CAANNNNNGTGG 2 cut(s) 319, 354
CviAII CATG 2 cut(s) 136, 179
CviJI RGCY 6 cut(s) 140, 202, 208, 254, 281, 299
CviKI_1 RGCY 6 cut(s) 140, 202, 208, 254, 281, 299
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 4 cut(s) 230, 255, 282, 312
DpnI GATC 2 cut(s) 183, 237
DpnII GATC 2 cut(s) 181, 235
Ecl136II GAGCTC 1 cut(s) 202
Eco24I GRGCYC 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 81
Eco53kI GAGCTC 1 cut(s) 202
Eco91I GGTNACC 1 cut(s) 271
EcoICRI GAGCTC 1 cut(s) 202
EcoO65I GGTNACC 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 129
EcoT38I GRGCYC 1 cut(s) 204
FaeI CATG 2 cut(s) 139, 182
FaiI YATR 2 cut(s) 137, 180
FatI CATG 2 cut(s) 135, 178
Fnu4HI GCNGC 3 cut(s) 300, 303, 306
FokI GGATG 1 cut(s) 155
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 3 cut(s) 300, 303, 306
GlaI GCGC 1 cut(s) 191
GluI GCNGC 3 cut(s) 300, 303, 306
GsuI CTGGAG 2 cut(s) 94, 239
HaeII RGCGCY 1 cut(s) 193
HgaI GACGC 2 cut(s) 133, 297
HhaI GCGC 1 cut(s) 192
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 2 cut(s) 139, 182
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 3 cut(s) 25, 226, 243
HphI GGTGA 2 cut(s) 265, 275
Hpy188I TCNGA 4 cut(s) 30, 231, 285, 313
Hpy188III TCNNGA 3 cut(s) 39, 58, 111
HpyAV CCTTC 1 cut(s) 262
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 214, 305
HpyF3I CTNAG 4 cut(s) 230, 255, 282, 312
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 2 cut(s) 139, 182
HspAI GCGC 1 cut(s) 190
Kzo9I GATC 2 cut(s) 181, 235
Lsp1109I GCAGC 2 cut(s) 286, 289
MaeIII GTNAC 3 cut(s) 18, 145, 271
MalI GATC 2 cut(s) 183, 237
MboI GATC 2 cut(s) 181, 235
MboII GAAGA 2 cut(s) 64, 166
MhlI GDGCHC 1 cut(s) 204
MlyI GAGTC 1 cut(s) 19
MnlI CCTC 8 cut(s) 26, 107, 115, 214, 244, 271, 279, 307
MroXI GAANNNNTTC 1 cut(s) 68
MspR9I CCNGG 1 cut(s) 131
MvaI CCWGG 1 cut(s) 131
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 3 cut(s) 141, 214, 305
NdeII GATC 2 cut(s) 181, 235
NlaIII CATG 2 cut(s) 139, 182
NlaIV GGNNCC 2 cut(s) 43, 328
NmuCI GTSAC 2 cut(s) 145, 271
PdmI GAANNNNTTC 1 cut(s) 68
PfeI GAWTC 2 cut(s) 226, 243
PflMI CCANNNNNTGG 1 cut(s) 136
PkrI GCNGC 3 cut(s) 301, 304, 307
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 129
PspEI GGTNACC 1 cut(s) 271
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 2 cut(s) 43, 328
PspPI GGNCC 1 cut(s) 81
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SacI GAGCTC 1 cut(s) 204
SatI GCNGC 3 cut(s) 300, 303, 306
Sau3AI GATC 2 cut(s) 181, 235
Sau96I GGNCC 1 cut(s) 81
SchI GAGTC 1 cut(s) 19
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 1 cut(s) 204
SetI ASST 7 cut(s) 83, 99, 204, 210, 273, 301, 325
SinI GGWCC 1 cut(s) 81
SmlI CTYRAG 2 cut(s) 173, 203
SmoI CTYRAG 2 cut(s) 173, 203
SsiI CCGC 1 cut(s) 306
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 129
TatI WGTACW 1 cut(s) 3
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 2 cut(s) 226, 243
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 2 cut(s) 145, 271
TseI GCWGC 2 cut(s) 299, 302
Tsp45I GTSAC 2 cut(s) 145, 271
TspDTI ATGAA 1 cut(s) 64
TspGWI ACGGA 2 cut(s) 24, 263
TspRI CASTG 1 cut(s) 337
Van91I CCANNNNNTGG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 81
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XmnI GAANNNNTTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.