Rh6CG528900

cwf21 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0002497
Physical Location & Seq
Reverse (-)
68260599 .. 68264175
3577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG528900.1

Sequence Viewer

Length: 2145 bp
ATGTACAACGGAATAGGGTTACAGACTCCGAGAGGGTCCGGAACCAATGGCTATATCCAGACGAACAAGTTCTTCATCAAATCCAAGACTGGCAAAGTCGCCGAGAACTCCAGAGGCTTCGAGGGCGACCAGGGCATGGCCGGCGTCACCAAGAAGCCCAACAGAGACATCCTTGAGCATGATCGCAAGCGCCAGATTGAGCTCAAGCTTGTCGTCCTCGAGGACAAGCTCACCGATCAGGGTTTCACCGACGCTGAGATTGCTGACAAGCTCGCCCAGGCCAGGAGCACTCTCGAAGCCGCCGCCGCCTCCGAGGACATGGGTGGACCCACTGCCTTTGTTCTTGGAGATAACAAGGTTTCAGATACACAGACCCACCAAATTGCTGCTAGGAAGCAGAAGCAGATGGACAACTTGAGAGATGCTTTGAAACTAAAACCTGAAGCTACAGAAACAAATACTGAGGAGATCGAGGATGGACTAATCATTAGTCCGAAGAATGATGATAGCAGGCATTATGAGAGGAAAGAGCATTCGTTTTTGGACAGAGAATCTGGGTGGAAGAAATCTGTGGAGGATCAGCACAAGGTTGAGAAAGATAAGAAAAAGAGGGTTAAAGAATCTAGGAGGAACAAAAAGAAAGAAACTAGGAAGAGAAGGCATAAGGATGATTCTTCTGATACAGATAGCAGTGGTAGCCCTCTGAAACCTGATAAAAAGAAAAAGCGTCGGAGTAGAAGTAGCAGCAGTGAAGAAGATGATTCTGAAGTTGATACATCGAAAAAGGAAGTTGAGAAATACAAAAAACGGCAGAAGAGGTACGATTCAGAAGAAGATGATTCTGAAGTTGATGTTGACAAGAAGCGGAAGGTTGTAAAGAAGCATAGTAAAAGCCGAAACAATAACACTGATGATTCTGATACCAGTGATGATGCGGAGGTCAGATCCAGGAAGGAAATTAAGAAATCCAAACAACCTTGTAGGAGGCATGATTCAGATGAAGATGATTCTGAAGATGATGTTGACAAGAAGAGGAAGGTTGTGATGAAGCATAGTAAGACTAGAAACGATGATACTGATGATTCTGCTTCTAGTGAAGATGCCGAGGATAGATCCACAAAGGAAGTCAAGAAATCCAAACAACCTCGTAGGAGGCATGATTCAGATGAAGATGGTTCTGAAGATGATGTTGACAAGAAGAGGAAGGTTGTAAAGAAGCATAGTAAAGGCAGAACCGATAATACTGATGATTCTGCTGCCAGTGAAGATGCCGAGGATAGATCCACAAAGGAAGTCAAGAAATTCCAACAACCTCGTAGGAGGCATGATTCAGATGATGATTCTGGTTATCCTGAAGAACTGCCAAAGAGCAGAACTGAAAAGGGCAAACAACATGTGAAACCAAACAAAAGGCATGACTCTGGGGATGAGTCTGATTTTGACAGCAGAATGGAAAGGAAAAGTAGCCAAGTTGAGCAGCAAAGGAATCAGCAGGTCAGCTGCAGGGTAGATCGAGATGAATTTGATGTGGAGCAAGCCGACAATGGAAAAAGCAGAAAATTGTATGATAGTGATTCTGACTATGAAAGATCCAGAAAAAGTAGAGGTGCAATGACAGAAAAGAGTAGAAGAAGTGGCAGGAATGATACTAATGACGATGATACTAATATTGGGAGGAAACCTGGAAGGGTTGAAGAGGGTGATGGAGGACGTGGAAGGCACAACAAGGGTGAAATGCAAAGAGGAAGGAAGAATGAAAGAGATGAAGAGGATCATGATTATAGAAGGCCCAGGGAAGGTCTATTACAGGAGCTTGGAAATAGAAAGCGTGGTACTAGAGAGGAAGAAGGGGCAAATGAGGATATTGAGAGGGATAGACATATGGATTACAAGAGAGCAAAATACGATGAGTCTAGATCAAGTGAGAGGAGAAGGCATGAAACTGACAGACATAATGAAGAGGAGCATAAGTCTAGAAGGCATGGAGAAGATGAAGGGGAGCGTAGAAACAGAAGACATGGAAAACTGGAGGAACAACAGGGAAATAAGGAGATTGAGAGAGATAGACAGGATTCCAGATCAAGTGAGAGAAGAAGGTATGAGAATGTCAAGCACAATGACGGTCGGTCCAGGCATCGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cwf21

Protein Analysis

714

Amino Acids

83.31

Weight (kDa)

8.82

Isoelectric Point (pI)

72.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf21 PF08312 58 - 100 1.7e-14 cwf21 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 218
Acc36I ACCTGC 1 cut(s) 1483
AccB7I CCANNNNNTGG 1 cut(s) 136
AccIII TCCGGA 1 cut(s) 38
AciI CCGC 5 cut(s) 300, 303, 306, 865, 935
AclWI GGATC 6 cut(s) 585, 939, 1107, 1275, 1584, 1779
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 2 cut(s) 1301, 1520
AcuI CTGAAG 6 cut(s) 462, 786, 864, 1032, 1200, 1374
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 3 cut(s) 5, 821, 1834
AfiI CCNNNNNNNGG 3 cut(s) 136, 282, 1796
AflIII ACRYGT 1 cut(s) 1393
AgsI TTSAA 2 cut(s) 430, 1694
AhdI GACNNNNNGTC 1 cut(s) 2125
AjiI CACGTC 1 cut(s) 1712
AjnI CCWGG 7 cut(s) 129, 276, 281, 947, 1681, 1790, 2129
AluBI AGCT 7 cut(s) 202, 208, 229, 271, 446, 1500, 1813
AluI AGCT 7 cut(s) 202, 208, 229, 271, 446, 1500, 1813
Alw21I GWGCWC 2 cut(s) 204, 290
Alw26I GTCTC 1 cut(s) 159
AlwI GGATC 6 cut(s) 585, 939, 1107, 1275, 1584, 1779
Ama87I CYCGRG 1 cut(s) 218
Aor13HI TCCGGA 1 cut(s) 38
AoxI GGCC 3 cut(s) 138, 279, 1787
ApeKI GCWGC 5 cut(s) 386, 744, 1256, 1477, 1500
ApoI RAATTY 2 cut(s) 1301, 1520
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 4 cut(s) 36, 326, 1788, 2127
AsuHPI GGTGA 5 cut(s) 139, 223, 238, 1712, 1742
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 3 cut(s) 36, 326, 2127
BanII GRGCYC 1 cut(s) 204
BarI GAAGNNNNNNTAC 2 cut(s) 1788, 1820
BbsI GAAGAC 1 cut(s) 2020
Bbv12I GWGCWC 2 cut(s) 204, 290
BbvI GCAGC 5 cut(s) 373, 756, 1243, 1487, 1489
BccI CCATC 4 cut(s) 400, 470, 1166, 1697
BceAI ACGGC 1 cut(s) 824
BciT130I CCWGG 7 cut(s) 131, 278, 283, 949, 1683, 1792, 2131
BcoDI GTCTC 1 cut(s) 159
BfaI CTAG 8 cut(s) 390, 624, 648, 1062, 1092, 1836, 1914, 1974
BfmI CTRYAG 2 cut(s) 447, 1501
BfoI RGCGCY 1 cut(s) 193
BfuAI ACCTGC 1 cut(s) 1483
BisI GCNGC 8 cut(s) 300, 303, 306, 387, 745, 1257, 1478, 1501
BlsI GCNGC 8 cut(s) 301, 304, 307, 388, 746, 1258, 1479, 1502
Bme1390I CCNGG 7 cut(s) 131, 278, 283, 949, 1683, 1792, 2131
Bme18I GGWCC 3 cut(s) 36, 326, 2127
BmeRI GACNNNNNGTC 1 cut(s) 2125
BmeT110I CYCGRG 1 cut(s) 218
BmgBI CACGTC 1 cut(s) 1712
BmgT120I GGNCC 4 cut(s) 36, 326, 1788, 2127
BmiI GGNNCC 3 cut(s) 37, 43, 328
BmrFI CCNGG 7 cut(s) 131, 278, 283, 949, 1683, 1792, 2131
BmsI GCATC 4 cut(s) 412, 922, 1090, 1258
BpiI GAAGAC 1 cut(s) 2020
BpmI CTGGAG 2 cut(s) 94, 2048
BpuEI CTTGAG 3 cut(s) 188, 194, 436
BsaHI GRCGYC 1 cut(s) 144
BsaJI CCNNGG 7 cut(s) 130, 276, 312, 1104, 1272, 1790, 1791
BsaWI WCCGGW 1 cut(s) 38
BsaXI ACNNNNNCTCC 4 cut(s) 724, 754, 1955, 1985
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 282, 1796
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 4 cut(s) 94, 924, 1260, 2031
Bse3DI GCAATG 1 cut(s) 1617
BseAI TCCGGA 1 cut(s) 38
BseBI CCWGG 7 cut(s) 131, 278, 283, 949, 1683, 1792, 2131
BseDI CCNNGG 7 cut(s) 130, 276, 312, 1104, 1272, 1790, 1791
BseGI GGATG 4 cut(s) 168, 481, 673, 1432
BseLI CCNNNNNNNGG 3 cut(s) 136, 282, 1796
BseMI GCAATG 1 cut(s) 1617
BseMII CTCAG 2 cut(s) 246, 453
BseNI ACTGG 4 cut(s) 94, 924, 1260, 2031
BseRI GAGGAG 3 cut(s) 479, 1942, 1976
BseXI GCAGC 5 cut(s) 373, 756, 1243, 1487, 1489
Bsh1285I CGRYCG 1 cut(s) 2125
BshFI GGCC 3 cut(s) 140, 281, 1789
BsiEI CGRYCG 1 cut(s) 2125
BsiHKAI GWGCWC 2 cut(s) 204, 290
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 2 cut(s) 39, 141
BslI CCNNNNNNNGG 3 cut(s) 136, 282, 1796
BsmAI GTCTC 1 cut(s) 159
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 3 cut(s) 140, 281, 1789
BsoBI CYCGRG 1 cut(s) 218
Bsp1286I GDGCHC 2 cut(s) 204, 290
Bsp13I TCCGGA 1 cut(s) 38
Bsp1407I TGTACA 1 cut(s) 3
BspACI CCGC 5 cut(s) 300, 303, 306, 865, 935
BspANI GGCC 3 cut(s) 140, 281, 1789
BspCNI CTCAG 2 cut(s) 247, 454
BspEI TCCGGA 1 cut(s) 38
BspHI TCATGA 1 cut(s) 1774
BspLI GGNNCC 3 cut(s) 37, 43, 328
BspMAI CTGCAG 1 cut(s) 1505
BspMI ACCTGC 1 cut(s) 1483
BspPI GGATC 6 cut(s) 585, 939, 1107, 1275, 1584, 1779
BsrDI GCAATG 1 cut(s) 1617
BsrFI RCCGGY 1 cut(s) 140
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 4 cut(s) 94, 924, 1260, 2031
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 7 cut(s) 130, 276, 312, 1104, 1272, 1790, 1791
BssNI GRCGYC 1 cut(s) 144
Bst2UI CCWGG 7 cut(s) 131, 278, 283, 949, 1683, 1792, 2131
Bst4CI ACNGT 1 cut(s) 2123
Bst6I CTCTTC 7 cut(s) 647, 809, 1025, 1193, 1689, 1761, 1953
BstACI GRCGYC 1 cut(s) 144
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 5 cut(s) 142, 188, 273, 512, 1536
BstDEI CTNAG 2 cut(s) 255, 462
BstF5I GGATG 4 cut(s) 168, 481, 673, 1432
BstH2I RGCGCY 1 cut(s) 193
BstHHI GCGC 1 cut(s) 192
BstMAI GTCTC 1 cut(s) 159
BstMCI CGRYCG 1 cut(s) 2125
BstMWI GCNNNNNNNGC 6 cut(s) 123, 132, 141, 260, 305, 696
BstNI CCWGG 7 cut(s) 131, 278, 283, 949, 1683, 1792, 2131
BstNSI RCATGY 1 cut(s) 1397
BstSCI CCNGG 7 cut(s) 129, 276, 281, 947, 1681, 1790, 2129
BstSFI CTRYAG 2 cut(s) 447, 1501
BstV1I GCAGC 5 cut(s) 373, 756, 1243, 1487, 1489
BstV2I GAAGAC 1 cut(s) 2020
BstX2I RGATCY 4 cut(s) 944, 1112, 1280, 1589
BstYI RGATCY 4 cut(s) 944, 1112, 1280, 1589
BsuRI GGCC 3 cut(s) 140, 281, 1789
BtrI CACGTC 1 cut(s) 1712
BtsCI GGATG 4 cut(s) 168, 481, 673, 1432
BtsI GCAGTG 3 cut(s) 330, 697, 754
BtsIMutI CAGTG 6 cut(s) 330, 697, 754, 906, 931, 1267
BveI ACCTGC 1 cut(s) 1483
Cac8I GCNNGC 5 cut(s) 142, 188, 273, 512, 1536
CciI TCATGA 1 cut(s) 1774
CfoI GCGC 1 cut(s) 192
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 4 cut(s) 36, 326, 1788, 2127
CseI GACGC 3 cut(s) 133, 260, 716
Csp6I GTAC 3 cut(s) 4, 820, 1833
CspCI CAANNNNNGTGG 2 cut(s) 319, 354
CviQI GTAC 3 cut(s) 4, 820, 1833
DdeI CTNAG 2 cut(s) 255, 462
DriI GACNNNNNGTC 1 cut(s) 2125
EaeI YGGCCR 1 cut(s) 138
Eam1104I CTCTTC 7 cut(s) 647, 809, 1025, 1193, 1689, 1761, 1953
Eam1105I GACNNNNNGTC 1 cut(s) 2125
EarI CTCTTC 7 cut(s) 647, 809, 1025, 1193, 1689, 1761, 1953
Ecl136II GAGCTC 1 cut(s) 202
Eco24I GRGCYC 1 cut(s) 204
Eco47I GGWCC 3 cut(s) 36, 326, 2127
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 6 cut(s) 462, 786, 864, 1032, 1200, 1374
Eco88I CYCGRG 1 cut(s) 218
EcoICRI GAGCTC 1 cut(s) 202
EcoRII CCWGG 7 cut(s) 129, 276, 281, 947, 1681, 1790, 2129
EcoT38I GRGCYC 1 cut(s) 204
FauNDI CATATG 1 cut(s) 1881
Fnu4HI GCNGC 8 cut(s) 300, 303, 306, 387, 745, 1257, 1478, 1501
FokI GGATG 4 cut(s) 155, 488, 680, 1439
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 8 cut(s) 300, 303, 306, 387, 745, 1257, 1478, 1501
FspBI CTAG 8 cut(s) 390, 624, 648, 1062, 1092, 1836, 1914, 1974
GlaI GCGC 1 cut(s) 191
GluI GCNGC 8 cut(s) 300, 303, 306, 387, 745, 1257, 1478, 1501
GsuI CTGGAG 2 cut(s) 94, 2048
HaeII RGCGCY 1 cut(s) 193
HaeIII GGCC 3 cut(s) 140, 281, 1789
HapII CCGG 2 cut(s) 39, 141
HgaI GACGC 3 cut(s) 133, 260, 716
HhaI GCGC 1 cut(s) 192
Hin1I GRCGYC 1 cut(s) 144
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HincII GTYRAC 3 cut(s) 856, 1024, 1192
HindII GTYRAC 3 cut(s) 856, 1024, 1192
HindIII AAGCTT 1 cut(s) 206
HpaII CCGG 2 cut(s) 39, 141
HphI GGTGA 5 cut(s) 139, 223, 238, 1712, 1742
Hpy166II GTNNAC 4 cut(s) 326, 856, 1024, 1192
Hpy8I GTNNAC 4 cut(s) 326, 856, 1024, 1192
Hpy99I CGWCG 2 cut(s) 254, 732
HpyCH4III ACNGT 1 cut(s) 2123
HpyCH4IV ACGT 1 cut(s) 1711
HpyCH4V TGCA 3 cut(s) 1503, 1610, 1738
HpyF10VI GCNNNNNNNGC 6 cut(s) 123, 132, 141, 260, 305, 696
HpyF3I CTNAG 2 cut(s) 255, 462
HpySE526I ACGT 1 cut(s) 1711
Hsp92I GRCGYC 1 cut(s) 144
HspAI GCGC 1 cut(s) 190
Kpn2I TCCGGA 1 cut(s) 38
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
LmnI GCTCC 5 cut(s) 285, 1531, 1810, 1963, 1999
Lsp1109I GCAGC 5 cut(s) 373, 756, 1243, 1487, 1489
LweI GCATC 4 cut(s) 412, 922, 1090, 1258
MaeI CTAG 8 cut(s) 390, 624, 648, 1062, 1092, 1836, 1914, 1974
MaeII ACGT 1 cut(s) 1711
MaeIII GTNAC 3 cut(s) 18, 145, 2138
MflI RGATCY 4 cut(s) 944, 1112, 1280, 1589
MhlI GDGCHC 2 cut(s) 204, 290
MluCI AATT 5 cut(s) 381, 957, 1301, 1520, 1559
MlyI GAGTC 4 cut(s) 19, 1412, 1439, 1919
MmeI TCCRAC 2 cut(s) 710, 1330
MroI TCCGGA 1 cut(s) 38
MroNI GCCGGC 1 cut(s) 140
MroXI GAANNNNTTC 1 cut(s) 68
MseI TTAA 2 cut(s) 615, 960
MslI CAYNNNNRTG 1 cut(s) 666
MspA1I CMGCKG 1 cut(s) 1500
MspI CCGG 2 cut(s) 39, 141
MspR9I CCNGG 7 cut(s) 131, 278, 283, 949, 1683, 1792, 2131
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 7 cut(s) 131, 278, 283, 949, 1683, 1792, 2131
MwoI GCNNNNNNNGC 6 cut(s) 123, 132, 141, 260, 305, 696
NaeI GCCGGC 1 cut(s) 142
NdeI CATATG 1 cut(s) 1881
NgoMIV GCCGGC 1 cut(s) 140
NlaIV GGNNCC 3 cut(s) 37, 43, 328
NmeAIII GCCGAG 3 cut(s) 127, 1129, 1297
NmuCI GTSAC 2 cut(s) 145, 2138
NspI RCATGY 1 cut(s) 1397
PaeR7I CTCGAG 1 cut(s) 218
PagI TCATGA 1 cut(s) 1774
PasI CCCWGGG 1 cut(s) 1791
PciI ACATGT 1 cut(s) 1393
PctI GAATGC 1 cut(s) 532
PdiI GCCGGC 1 cut(s) 142
PdmI GAANNNNTTC 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 136
PfoI TCCNGGA 1 cut(s) 947
PkrI GCNGC 8 cut(s) 301, 304, 307, 388, 746, 1258, 1479, 1502
PleI GAGTC 4 cut(s) 19, 1412, 1438, 1918
PpsI GAGTC 4 cut(s) 19, 1412, 1438, 1918
PscI ACATGT 1 cut(s) 1393
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 7 cut(s) 129, 276, 281, 947, 1681, 1790, 2129
PspGI CCWGG 7 cut(s) 129, 276, 281, 947, 1681, 1790, 2129
PspN4I GGNNCC 3 cut(s) 37, 43, 328
PspPI GGNCC 4 cut(s) 36, 326, 1788, 2127
PspXI VCTCGAGB 1 cut(s) 218
PsrI GAACNNNNNNTAC 2 cut(s) 1225, 1257
PstI CTGCAG 1 cut(s) 1505
PsuI RGATCY 4 cut(s) 944, 1112, 1280, 1589
PvuII CAGCTG 1 cut(s) 1500
RsaI GTAC 3 cut(s) 5, 821, 1834
RsaNI GTAC 3 cut(s) 4, 820, 1833
RseI CAYNNNNRTG 1 cut(s) 666
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 2 cut(s) 615, 960
SatI GCNGC 8 cut(s) 300, 303, 306, 387, 745, 1257, 1478, 1501
Sau96I GGNCC 4 cut(s) 36, 326, 1788, 2127
SchI GAGTC 4 cut(s) 19, 1412, 1439, 1919
ScrFI CCNGG 7 cut(s) 131, 278, 283, 949, 1683, 1792, 2131
SduI GDGCHC 2 cut(s) 204, 290
SfaNI GCATC 4 cut(s) 412, 922, 1090, 1258
SfcI CTRYAG 2 cut(s) 447, 1501
Sfr274I CTCGAG 1 cut(s) 218
SinI GGWCC 3 cut(s) 36, 326, 2127
SlaI CTCGAG 1 cut(s) 218
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 4 cut(s) 173, 203, 218, 415
SmoI CTYRAG 4 cut(s) 173, 203, 218, 415
Sse9I AATT 5 cut(s) 381, 957, 1301, 1520, 1559
SsiI CCGC 5 cut(s) 300, 303, 306, 865, 935
SspI AATATT 1 cut(s) 1669
SspMI CTAG 8 cut(s) 390, 624, 648, 1062, 1092, 1836, 1914, 1974
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 7 cut(s) 129, 276, 281, 947, 1681, 1790, 2129
TaaI ACNGT 1 cut(s) 2123
TaiI ACGT 1 cut(s) 1714
TaqI TCGA 6 cut(s) 120, 219, 294, 471, 779, 1513
TaqII GACCGA 1 cut(s) 2115
TasI AATT 5 cut(s) 381, 957, 1301, 1520, 1559
TatI WGTACW 1 cut(s) 3
TauI GCSGC 3 cut(s) 302, 305, 308
Tru1I TTAA 2 cut(s) 615, 960
Tru9I TTAA 2 cut(s) 615, 960
TscAI CASTG 6 cut(s) 337, 697, 754, 913, 931, 1267
TseFI GTSAC 2 cut(s) 145, 2138
TseI GCWGC 5 cut(s) 386, 744, 1256, 1477, 1500
Tsp45I GTSAC 2 cut(s) 145, 2138
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 6 cut(s) 337, 697, 754, 913, 931, 1267
Van91I CCANNNNNTGG 1 cut(s) 136
VpaK11BI GGWCC 3 cut(s) 36, 326, 2127
XapI RAATTY 2 cut(s) 1301, 1520
XbaI TCTAGA 2 cut(s) 1913, 1973
XceI RCATGY 1 cut(s) 1397
XhoI CTCGAG 1 cut(s) 218
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 8 cut(s) 390, 624, 648, 1062, 1092, 1836, 1914, 1974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.