Rorug06G0395400

cwf21 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002497
Physical Location & Seq
Forward (+)
54070115 .. 54077061
6947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0395400.1

Sequence Viewer

Length: 4218 bp
ATGGAAGTTTTCTCCAAGTCTTCTCGTGAAGAAGACGATGAAGAAGCTCTGAAATGGGCTGCTATTGAGAGATTGCCAACTTACTTGCGCATAAGGAGAGGCATACTCACGGACCAAGACGCGGGTAAAGCCAGAGAAATTGATGTCAAAAATTTAGGACTTTTAGAAAGGAGGAATTTGCTGGAGAGGCTGGTGAAGATTGCAGATGAAGATAATGAGAAGTTCTTGTTCAAGCTCAGGGACCGCATGTATAGAGTTGGACTTGAATTTCCAACAACTGAGGTGCGGTTTGAGCATTTAAGTGTCGAAGCAGAAGCTTATGTAGGGAGTAGGGCATTACCTACAATGCTCAACTTCTCCATTAACATCTTAGAGGGGTTGTTGAATTACCTTCATGTTCTTCCAAGTAGAAAGAAGCGATTGCCAATCCTCCACGGTGTTAGTGGAATTATCAAGCCAAGAAGAATGACACTGCTTTTAGGCCCTCCAAGCTCTGGAAAGACCACATTACTACTGGCTTTGGCTGGAAAACTCGGTAAAGATCTGAAACTTTCGGGGAGAGTTACATACAATGGAGAGGGGATGGAAGAGTTTGTACCAGAGAGGACATCAGCATATATTAGTCAACATGATCTTCACATACCAGAGATGACAGTGAGAGAAACTCTGGCTTTTTCAGCAAGATGTCAAGGAGTTGGACCACGTTATGAAATGTTGGTAGAATTATCAAGGAGAGAAAAGACTGCAAATATAATGCCAGATCCTGATCTTGATGTTTACATGAAGGCAGCTGCACTAGAAGGACAGGAAACCAATGTAATTACAGATTATATACTCAAGGTTTTGGGGCTTGAAGTTTGTGCTGACACTATAGTAGGGGATCAAATGAGGCGAGGTATCTCTGGGGGACAAAGAAAGCGACTCACCACAGGGGAGATGCTGGTTGGACCAGCAAGAGCACTTTTTATGGATGAGATATCCACTGGCTTGGACAGTTCAACAACATTTCAGATAGTGAAGTCACTTAGACAATCCATCCACATTCTCAATGGAACTGCTGTCATCTCTCTCCTGCAGCCAGCGCCAGAAACTTATGATCTTTTCGATGATATAATTCTACTCTCAGATGGGCACATTGTGTATCAAGGTCCCCGGGACAATGTGCTTGAGTTCTTTGAGTACATGGGCTTTAAATGTCCAGAGAGAAAAGGAGTTGCTGATTTTCTACAAGAAGTAACCTCAGGGAAAGATCAAGAGCAATACTGGGCTGATAAAGATAAGCCTTTTATCTTTGTAACTTCCAAGAACTTTGCTGAAGCCTTGCAATCATTTTACATTGGTCGAGAACTTGGTGATGAGCTTTCTATTCCGTTTGACAAGTCTAAAGGTCATCCTGCAGCTTTAACAACCAAGAAATATGGCGTCAGCAAGAAGGAATTATTCAAAGCTTGTATGTCTAGGCAAATTTTGCTTATGAAGAGGAATTCATTTATCCACATTTTCAAAATTTCACAGATGATTCTATTGTCTTGTGTAACAATAACATTATTCCTACGAACTGAGATGCACCGAGATACTGTTGAAGATGGTGGGATATATATGGGAGCATTGTTTTACTCAATGATGATAATTATGTTCAATGGGTTCTCAGAGCTTGCTATGACTGTCATGAAGCTTCCTGTGTTTTTCAAGCAAAGGGACCTTCTCTTCTATCCTGCTTGGGCGTACTCTTTGCCCACATGGCTGATAAGGATCCCTATGACTTTTGTGGAAGTTTCCATTTGGGTGACCATTACGTACTACACTAGGTTCTTCAAACAGTTCCTTTCACTGCTGTTTATTAGCCAAATGGCAACTGGACTGTTCCGACTGATAGGGGCATTAGGAAGAAACATGATTGTTGCAAACACATTTGGATCGGTTGCTTTGCTTGTGCTGTTTGTTCTTGGTGGATTCCTTTTATCACGAGAGGATATGAAAAGCTGGTTGGTGTGGGGATATTATCTGTCACCAATGTCGTATGGACTGAATGCCCTAGCTGTCAATGAATTTCTTGGAAAAAGTTGGAGACATGTTTCTACCAACTCAAAAGAACCATTAGGAATAATGGTCTTGAATTCTCGAGGGGTGTTCCCAGAAGCACGTTGGTACTGGATTGGAGTAGTAGCTTTATTTGGATTTATTCTTCTATTCAACTTCCTTTTCATTTTGGCACTTCAGTATCTTGAACCTCTTGATAAGCCTCAGGCAGTACTTTCCAAAGAGGCCTTGGCAAAGAAACAAGCCACTGAAACTGGAGAGGCCATTGAATTATTGTCAACAGGAAAGAGCTATATTGGTGCAGCTAATGAAAGACTAGACAATGTGTCAGTCAATCTTACTGGCCAGAGAGGAATGGTTCTTCCTTTTGAACCCCTCACAATTACATTTGATGAAATCAGATATGCAGTTGACATGCCACAGGAAATGAAAGCTCAAGGCATAACAGAGAATCGGTTAGAACTTCTGAAGGGTGTAAGTGGTGCTTTTAGGCCTGGAGTCCTAACAGCTCTAATGGGGGTCAGTGGCGCTGGAAAGACTACTCTAATGGATGTCCTGGCAGGAAGGAAAACTGGTGGACATATTGAAGGAAACATTACAATTTCTGGCTATCCAAAGAAGCAAGAAACATTTGCTCGTATATCTGGATATTGTGAACAAACGGATATACACTCTCCACATGTTACAGTCTATGAATCTTTGGTTTATTCTGCTTGGCTCCGGTTGCCCCCTGAGGTTGATTCCCCCACCAGAAAGATGTTTATTGAGGAAATCATGGAACTTGTGGAACTGACCCCAATAAGAGATGCACTTGTTGGATTGCCTGGAGTGAATGGGCTCTCAACTGAGCAGAGCAAAAGACTAACAATCGCAGTAGAGCTTGTTGCCAACCCATCCATAATATTTATGGATGAGCCAACCTCTGGTCTTGATGCCAGGGCTGCAGCAATTGTAATGAGAACTGTGAGGAATACAATGAACACCGGGAGAACTGTAGTCTGCACCATCCACCAGCCAAGCATTGATATATTTGATGCTTTTGATGAGATGCTACTTTTGAAATGGGGAGGAGAAGAAATATATGTTGGTCCTTTAGGCCACCAGTCCTCTCAGTTGATCGAATACTTTGAGGGGATCAGTGGAGTTCCTAAAATGAATGATGGTTACAATCCTGCAACTTGGATGTTAGAGGTTACTTCTGCAGCACAAGAATCAACTCTTGGGGTCAATTTCACGGACATATACAAGAACTCAGAAATGTACAGGAGAAACAAGGAATTGATCAAGGAACTAAGTACTCCACCAGAAAATTCAGTAGAATTGCACTTCACAACACAGTATTCTCAGTCCTTCTTTACCCAATGCATAACTTGCCTATGGAAACAACATCTGTCTTACTGGAGAAACCCGCCATACAGTGCAGCGAGACTTATTTACACAACTATGATGGCTATAGTATTTGGGATATTATTCTGGGGTCTTGGCTCCAAAAGGCAAAAGCAACAAGATCTTTTCAATGTATTAGGCTCTATGTATTCTGCCATACTCTTCATTGGGATACAAAATGCCTCATCAGTGCAGCCAGTTATAGCGATTGAGAGGGTAGTCTTTTACAGAGAAAGGGCAGCTGGAATGTACTCTGCTTTTCCATATGCATTTGGACAGGTTGTCATTGAGATACCATACACTTTGATCCAAACCACATTATATGTGGTTATAGTATACACCATGATCGGATATGATTGGACGGTCAGCAAGTTTGTCTGGTATTTTTTCTTCATGTATTTCAGCTTCTTATACTACACATTATATGGTATGATGATTGTGGCCATTACTCCCAACACTATGGTTGCTGCTGTGCTTTCCTCATCCTTCTACCCATTATGGAACGTCTTTTCTGGATTTATCATTCCCAAAACAAGAATTCCAATATGGTGGAGATGGTTCTATTGGTTGTGCCCGATTTCTTGGACCTTATACGGATTGATTGTTTCGCAGTTTGGAGACATTAAGGACGCACTTGAATCTGGCAAAACTGTGGAGAATTTTATTAGTGATTATTTTGGGTACAAAAAAGACTTTTTAGGTGTTGTTGCAATTGTGCATGTTGGGATTGCAGTGCTCTTTGGTTTCATCTTTGCCTTTTCAATCAAAGCGTTTAATTTCCAAAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cwf21

Protein Analysis

1405

Amino Acids

159.24

Weight (kDa)

7.05

Isoelectric Point (pI)

40.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 69 - 119 8.1e-07 ABC-transporter N-terminal
ABC_tran PF00005 144 - 325 1.3e-14 ABC transporter
ABC2_membrane_7 PF19055 359 - 416 6.6e-06 ABC-2 type transporter
ABC2_membrane PF01061 480 - 684 4.6e-42 ABC-2 type transporter
PDR_assoc PF08370 689 - 751 2.1e-27 Plant PDR ABC transporter associated
ABC_tran PF00005 836 - 988 6.3e-20 ABC transporter
ABC2_membrane PF01061 1133 - 1347 3.4e-60 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 89
AccB7I CCANNNNNTGG 2 cut(s) 494, 2963
AccI GTMKAC 1 cut(s) 3762
AccII CGCG 1 cut(s) 122
AciI CCGC 4 cut(s) 122, 244, 286, 3449
AclWI GGATC 7 cut(s) 755, 888, 1747, 1760, 1924, 3182, 3727
AcoI YGGCCR 2 cut(s) 2383, 3867
AcuI CTGAAG 3 cut(s) 1335, 2201, 2528
AcyI GRCGYC 1 cut(s) 1422
AdeI CACNNNGTG 1 cut(s) 1138
AfiI CCNNNNNNNGG 3 cut(s) 121, 494, 2963
AflIII ACRYGT 2 cut(s) 2071, 2719
AhdI GACNNNNNGTC 2 cut(s) 2365, 3707
AjnI CCWGG 4 cut(s) 2533, 2595, 2863, 2975
AjuI GAANNNNNNNTTGG 6 cut(s) 1970, 2002, 3108, 3140, 3243, 3275
Alw21I GWGCWC 2 cut(s) 961, 4164
Alw26I GTCTC 3 cut(s) 2062, 3460, 4038
AlwI GGATC 7 cut(s) 755, 888, 1747, 1760, 1924, 3182, 3727
AlwNI CAGNNNCTG 1 cut(s) 764
Ama87I CYCGRG 2 cut(s) 1152, 2121
AoxI GGCC 7 cut(s) 481, 2265, 2301, 2383, 2531, 3136, 3867
AspLEI GCGC 3 cut(s) 90, 1084, 2570
AspS9I GGNCC 9 cut(s) 112, 241, 482, 698, 947, 1148, 1699, 3128, 4011
AsuC2I CCSGG 3 cut(s) 1153, 1154, 3025
AsuHPI GGTGA 5 cut(s) 205, 916, 1364, 1798, 2001
AvaI CYCGRG 2 cut(s) 1152, 2121
AvaII GGWCC 8 cut(s) 112, 241, 698, 947, 1148, 1699, 3128, 4011
AxyI CCTNAGG 3 cut(s) 1240, 2244, 2772
BaeGI GKGCMC 2 cut(s) 1134, 4001
BaeI ACNNNNGTAYC 2 cut(s) 2204, 2237
BalI TGGCCA 2 cut(s) 2385, 3869
BamHI GGATCC 1 cut(s) 1752
BanII GRGCYC 1 cut(s) 2880
BarI GAAGNNNNNNTAC 6 cut(s) 579, 611, 3800, 3815, 3832, 3847
BauI CACGAG 2 cut(s) 24, 1965
BbsI GAAGAC 2 cut(s) 12, 39
Bbv12I GWGCWC 2 cut(s) 961, 4164
BccI CCATC 9 cut(s) 577, 1043, 1121, 1580, 2941, 3053, 3194, 3481, 3975
BciT130I CCWGG 4 cut(s) 2535, 2597, 2865, 2977
BciVI GTATCC 1 cut(s) 3591
BclI TGATCA 1 cut(s) 3321
BcnI CCSGG 3 cut(s) 1153, 1154, 3025
BcoDI GTCTC 3 cut(s) 2062, 3460, 4038
BfaI CTAG 5 cut(s) 797, 1458, 1806, 2036, 2357
BfmI CTRYAG 7 cut(s) 870, 1073, 1395, 2982, 3033, 3240, 3492
BfoI RGCGCY 2 cut(s) 1085, 2571
BfuI GTATCC 1 cut(s) 3591
BglI GCCNNNNNGGC 1 cut(s) 1741
BglII AGATCT 2 cut(s) 541, 3547
BmcAI AGTACT 2 cut(s) 2253, 3337
Bme1390I CCNGG 7 cut(s) 1153, 1154, 2535, 2597, 2865, 2977, 3025
Bme18I GGWCC 8 cut(s) 112, 241, 698, 947, 1148, 1699, 3128, 4011
BmeRI GACNNNNNGTC 2 cut(s) 2365, 3707
BmeT110I CYCGRG 2 cut(s) 1152, 2121
BmgT120I GGNCC 9 cut(s) 112, 241, 482, 698, 947, 1148, 1699, 3128, 4011
BmiI GGNNCC 6 cut(s) 242, 1150, 1700, 1754, 2759, 3526
BmrFI CCNGG 7 cut(s) 1153, 1154, 2535, 2597, 2865, 2977, 3025
BmrI ACTGGG 1 cut(s) 1273
BmsI GCATC 6 cut(s) 927, 1554, 2836, 2962, 3064, 3078
BmuI ACTGGG 1 cut(s) 1273
BpiI GAAGAC 2 cut(s) 12, 39
BplI GAGNNNNNCTC 6 cut(s) 90, 122, 649, 681, 2945, 2977
BpmI CTGGAG 5 cut(s) 203, 2316, 2556, 2886, 3460
Bpu10I CCTNAGC 1 cut(s) 236
BpuEI CTTGAG 3 cut(s) 821, 1187, 2460
BpuMI CCSGG 3 cut(s) 1153, 1154, 3025
BsaAI YACGTR 1 cut(s) 1797
BsaBI GATNNNNATC 2 cut(s) 765, 1751
BsaHI GRCGYC 1 cut(s) 1422
BsaJI CCNNGG 5 cut(s) 433, 1151, 1152, 2268, 2976
BsaWI WCCGGW 1 cut(s) 2760
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 494, 2963
Bse21I CCTNAGG 3 cut(s) 1240, 2244, 2772
Bse8I GATNNNNATC 2 cut(s) 765, 1751
BseBI CCWGG 4 cut(s) 2535, 2597, 2865, 2977
BseDI CCNNGG 5 cut(s) 433, 1151, 1152, 2268, 2976
BseJI GATNNNNATC 2 cut(s) 765, 1751
BseLI CCNNNNNNNGG 3 cut(s) 121, 494, 2963
BseRI GAGGAG 1 cut(s) 3123
BseSI GKGCMC 2 cut(s) 1134, 4001
BsgI GTGCAG 5 cut(s) 777, 2361, 3025, 3480, 3638
Bsh1236I CGCG 1 cut(s) 122
BshFI GGCC 7 cut(s) 483, 2267, 2303, 2385, 2533, 3138, 3869
BsiHKAI GWGCWC 2 cut(s) 961, 4164
BsiHKCI CYCGRG 2 cut(s) 1152, 2121
BsiSI CCGG 3 cut(s) 1153, 2761, 3024
BslFI GGGAC 5 cut(s) 254, 921, 1134, 1169, 1712
BslI CCNNNNNNNGG 3 cut(s) 121, 494, 2963
BsmAI GTCTC 3 cut(s) 2062, 3460, 4038
BsmFI GGGAC 5 cut(s) 254, 921, 1134, 1169, 1712
BsmI GAATGC 1 cut(s) 2035
BsnI GGCC 7 cut(s) 483, 2267, 2303, 2385, 2533, 3138, 3869
BsoBI CYCGRG 2 cut(s) 1152, 2121
Bsp1286I GDGCHC 5 cut(s) 961, 1134, 2880, 4001, 4164
Bsp1407I TGTACA 1 cut(s) 3300
BspACI CCGC 4 cut(s) 122, 244, 286, 3449
BspANI GGCC 7 cut(s) 483, 2267, 2303, 2385, 2533, 3138, 3869
BspFNI CGCG 1 cut(s) 122
BspHI TCATGA 1 cut(s) 1668
BspLI GGNNCC 6 cut(s) 242, 1150, 1700, 1754, 2759, 3526
BspMAI CTGCAG 4 cut(s) 1077, 1399, 2986, 3244
BspPI GGATC 7 cut(s) 755, 888, 1747, 1760, 1924, 3182, 3727
BsrGI TGTACA 1 cut(s) 3300
BssECI CCNNGG 5 cut(s) 433, 1151, 1152, 2268, 2976
BssNAI GTATAC 1 cut(s) 3763
BssNI GRCGYC 1 cut(s) 1422
BssSI CACGAG 2 cut(s) 24, 1965
BssT1I CCWWGG 1 cut(s) 2268
Bst1107I GTATAC 1 cut(s) 3763
Bst2BI CACGAG 2 cut(s) 24, 1965
Bst2UI CCWGG 4 cut(s) 2535, 2597, 2865, 2977
Bst6I CTCTTC 4 cut(s) 582, 1472, 1712, 3593
BstACI GRCGYC 1 cut(s) 1422
BstAPI GCANNNNNTGC 2 cut(s) 1468, 3411
BstAUI TGTACA 1 cut(s) 3300
BstBAI YACGTR 1 cut(s) 1797
BstC8I GCNNGC 2 cut(s) 1080, 1656
BstDSI CCRYGG 1 cut(s) 433
BstEII GGTNACC 1 cut(s) 1786
BstFNI CGCG 1 cut(s) 122
BstH2I RGCGCY 2 cut(s) 1085, 2571
BstHHI GCGC 3 cut(s) 90, 1084, 2570
BstMAI GTCTC 3 cut(s) 2062, 3460, 4038
BstNI CCWGG 4 cut(s) 2535, 2597, 2865, 2977
BstNSI RCATGY 5 cut(s) 250, 2075, 2458, 2723, 4148
BstPI GGTNACC 1 cut(s) 1786
BstSCI CCNGG 7 cut(s) 1151, 1152, 2533, 2595, 2863, 2975, 3023
BstSFI CTRYAG 7 cut(s) 870, 1073, 1395, 2982, 3033, 3240, 3492
BstSLI GKGCMC 2 cut(s) 1134, 4001
BstSNI TACGTA 1 cut(s) 1797
BstUI CGCG 1 cut(s) 122
BstV2I GAAGAC 2 cut(s) 12, 39
BstX2I RGATCY 4 cut(s) 541, 760, 1752, 3547
BstXI CCANNNNNNTGG 3 cut(s) 988, 3886, 3975
BstYI RGATCY 4 cut(s) 541, 760, 1752, 3547
BstZ17I GTATAC 1 cut(s) 3763
Bsu36I CCTNAGG 3 cut(s) 1240, 2244, 2772
BsuI GTATCC 1 cut(s) 3591
BsuRI GGCC 7 cut(s) 483, 2267, 2303, 2385, 2533, 3138, 3869
BtgI CCRYGG 1 cut(s) 433
BtsI GCAGTG 3 cut(s) 470, 1829, 4164
Cac8I GCNNGC 2 cut(s) 1080, 1656
CaiI CAGNNNCTG 1 cut(s) 764
CciI TCATGA 1 cut(s) 1668
CfoI GCGC 3 cut(s) 90, 1084, 2570
Cfr13I GGNCC 9 cut(s) 112, 241, 482, 698, 947, 1148, 1699, 3128, 4011
Cfr9I CCCGGG 1 cut(s) 1152
CseI GACGC 3 cut(s) 128, 1411, 4064
DraI TTTAAA 1 cut(s) 1192
DraIII CACNNNGTG 1 cut(s) 1138
DriI GACNNNNNGTC 2 cut(s) 2365, 3707
EaeI YGGCCR 2 cut(s) 2383, 3867
Eam1104I CTCTTC 4 cut(s) 582, 1472, 1712, 3593
Eam1105I GACNNNNNGTC 2 cut(s) 2365, 3707
EarI CTCTTC 4 cut(s) 582, 1472, 1712, 3593
Eco105I TACGTA 1 cut(s) 1797
Eco130I CCWWGG 1 cut(s) 2268
Eco147I AGGCCT 2 cut(s) 2267, 2533
Eco24I GRGCYC 1 cut(s) 2880
Eco32I GATATC 1 cut(s) 978
Eco47I GGWCC 8 cut(s) 112, 241, 698, 947, 1148, 1699, 3128, 4011
Eco57I CTGAAG 3 cut(s) 1335, 2201, 2528
Eco81I CCTNAGG 3 cut(s) 1240, 2244, 2772
Eco88I CYCGRG 2 cut(s) 1152, 2121
Eco91I GGTNACC 1 cut(s) 1786
EcoO109I RGGNCCY 3 cut(s) 482, 1148, 1699
EcoO65I GGTNACC 1 cut(s) 1786
EcoRI GAATTC 3 cut(s) 1483, 2116, 3963
EcoRII CCWGG 4 cut(s) 2533, 2595, 2863, 2975
EcoRV GATATC 1 cut(s) 978
EcoT14I CCWWGG 1 cut(s) 2268
EcoT22I ATGCAT 2 cut(s) 3407, 3697
EcoT38I GRGCYC 1 cut(s) 2880
ErhI CCWWGG 1 cut(s) 2268
FalI AAGNNNNNCTT 2 cut(s) 2509, 2541
FaqI GGGAC 5 cut(s) 254, 921, 1134, 1169, 1712
FauI CCCGC 2 cut(s) 115, 3456
FauNDI CATATG 1 cut(s) 3691
FbaI TGATCA 1 cut(s) 3321
FblI GTMKAC 1 cut(s) 3762
FriOI GRGCYC 1 cut(s) 2880
FspBI CTAG 5 cut(s) 797, 1458, 1806, 2036, 2357
FspI TGCGCA 1 cut(s) 89
GlaI GCGC 3 cut(s) 89, 1083, 2569
GsuI CTGGAG 5 cut(s) 203, 2316, 2556, 2886, 3460
HaeII RGCGCY 2 cut(s) 1085, 2571
HaeIII GGCC 7 cut(s) 483, 2267, 2303, 2385, 2533, 3138, 3869
HapII CCGG 3 cut(s) 1153, 2761, 3024
HgaI GACGC 3 cut(s) 128, 1411, 4064
HhaI GCGC 3 cut(s) 90, 1084, 2570
Hin1I GRCGYC 1 cut(s) 1422
Hin6I GCGC 3 cut(s) 88, 1082, 2568
HinP1I GCGC 3 cut(s) 88, 1082, 2568
HincII GTYRAC 3 cut(s) 626, 2319, 2452
HindII GTYRAC 3 cut(s) 626, 2319, 2452
HindIII AAGCTT 3 cut(s) 315, 1446, 1673
HinfI GANTC 9 cut(s) 921, 1519, 1953, 2491, 2538, 2735, 2780, 3251, 4064
HpaII CCGG 3 cut(s) 1153, 2761, 3024
HphI GGTGA 5 cut(s) 205, 916, 1364, 1798, 2001
Hpy166II GTNNAC 7 cut(s) 626, 778, 2319, 2452, 2618, 2696, 3763
Hpy8I GTNNAC 7 cut(s) 626, 778, 2319, 2452, 2618, 2696, 3763
HpyCH4IV ACGT 4 cut(s) 703, 1796, 2143, 3930
HpySE526I ACGT 4 cut(s) 703, 1796, 2143, 3930
Hsp92I GRCGYC 1 cut(s) 1422
HspAI GCGC 3 cut(s) 88, 1082, 2568
Ksp22I TGATCA 1 cut(s) 3321
LmnI GCTCC 3 cut(s) 1604, 2763, 3530
LweI GCATC 6 cut(s) 927, 1554, 2836, 2962, 3064, 3078
MaeI CTAG 5 cut(s) 797, 1458, 1806, 2036, 2357
MaeII ACGT 4 cut(s) 703, 1796, 2143, 3930
MfeI CAATTG 2 cut(s) 2988, 4137
MflI RGATCY 4 cut(s) 541, 760, 1752, 3547
MhlI GDGCHC 5 cut(s) 961, 1134, 2880, 4001, 4164
MlsI TGGCCA 2 cut(s) 2385, 3869
MluNI TGGCCA 2 cut(s) 2385, 3869
MlyI GAGTC 2 cut(s) 915, 2547
MmeI TCCRAC 7 cut(s) 238, 296, 676, 925, 1891, 2045, 2836
Mox20I TGGCCA 2 cut(s) 2385, 3869
Mph1103I ATGCAT 2 cut(s) 3407, 3697
MscI TGGCCA 2 cut(s) 2385, 3869
MseI TTAA 6 cut(s) 299, 363, 1191, 1403, 4050, 4200
MslI CAYNNNNRTG 3 cut(s) 300, 1784, 3482
Msp20I TGGCCA 2 cut(s) 2385, 3869
MspA1I CMGCKG 2 cut(s) 791, 3668
MspI CCGG 3 cut(s) 1153, 2761, 3024
MspR9I CCNGG 7 cut(s) 1153, 1154, 2535, 2597, 2865, 2977, 3025
MunI CAATTG 2 cut(s) 2988, 4137
Mva1269I GAATGC 1 cut(s) 2035
MvaI CCWGG 4 cut(s) 2535, 2597, 2865, 2977
MvnI CGCG 1 cut(s) 122
NciI CCSGG 3 cut(s) 1153, 1154, 3025
NdeI CATATG 1 cut(s) 3691
NlaIV GGNNCC 6 cut(s) 242, 1150, 1700, 1754, 2759, 3526
NmuCI GTSAC 3 cut(s) 1020, 1786, 2007
NsbI TGCGCA 1 cut(s) 89
NsiI ATGCAT 2 cut(s) 3407, 3697
NspI RCATGY 5 cut(s) 250, 2075, 2458, 2723, 4148
PaeR7I CTCGAG 1 cut(s) 2121
PagI TCATGA 1 cut(s) 1668
PceI AGGCCT 2 cut(s) 2267, 2533
PciI ACATGT 2 cut(s) 2071, 2719
PctI GAATGC 1 cut(s) 2035
PfeI GAWTC 7 cut(s) 1519, 1953, 2491, 2735, 2780, 3251, 4064
PflMI CCANNNNNTGG 2 cut(s) 494, 2963
PleI GAGTC 2 cut(s) 915, 2546
PpsI GAGTC 2 cut(s) 915, 2546
Ppu21I YACGTR 1 cut(s) 1797
PpuMI RGGWCCY 2 cut(s) 1148, 1699
PscI ACATGT 2 cut(s) 2071, 2719
Psp5II RGGWCCY 2 cut(s) 1148, 1699
Psp6I CCWGG 4 cut(s) 2533, 2595, 2863, 2975
PspEI GGTNACC 1 cut(s) 1786
PspGI CCWGG 4 cut(s) 2533, 2595, 2863, 2975
PspN4I GGNNCC 6 cut(s) 242, 1150, 1700, 1754, 2759, 3526
PspPI GGNCC 9 cut(s) 112, 241, 482, 698, 947, 1148, 1699, 3128, 4011
PspPPI RGGWCCY 2 cut(s) 1148, 1699
PstI CTGCAG 4 cut(s) 1077, 1399, 2986, 3244
PstNI CAGNNNCTG 1 cut(s) 764
PsuI RGATCY 4 cut(s) 541, 760, 1752, 3547
PvuII CAGCTG 2 cut(s) 791, 3668
RseI CAYNNNNRTG 3 cut(s) 300, 1784, 3482
SaqAI TTAA 6 cut(s) 299, 363, 1191, 1403, 4050, 4200
Sau96I GGNCC 9 cut(s) 112, 241, 482, 698, 947, 1148, 1699, 3128, 4011
ScaI AGTACT 2 cut(s) 2253, 3337
SchI GAGTC 2 cut(s) 915, 2547
ScrFI CCNGG 7 cut(s) 1153, 1154, 2535, 2597, 2865, 2977, 3025
SduI GDGCHC 5 cut(s) 961, 1134, 2880, 4001, 4164
SfaNI GCATC 6 cut(s) 927, 1554, 2836, 2962, 3064, 3078
SfcI CTRYAG 7 cut(s) 870, 1073, 1395, 2982, 3033, 3240, 3492
Sfr274I CTCGAG 1 cut(s) 2121
SinI GGWCC 8 cut(s) 112, 241, 698, 947, 1148, 1699, 3128, 4011
SlaI CTCGAG 1 cut(s) 2121
SmaI CCCGGG 1 cut(s) 1154
SmiMI CAYNNNNRTG 3 cut(s) 300, 1784, 3482
SmlI CTYRAG 4 cut(s) 836, 1166, 2121, 2475
SmoI CTYRAG 4 cut(s) 836, 1166, 2121, 2475
SnaBI TACGTA 1 cut(s) 1797
SseBI AGGCCT 2 cut(s) 2267, 2533
SsiI CCGC 4 cut(s) 122, 244, 286, 3449
SspI AATATT 1 cut(s) 2943
SspMI CTAG 5 cut(s) 797, 1458, 1806, 2036, 2357
StuI AGGCCT 2 cut(s) 2267, 2533
StyD4I CCNGG 7 cut(s) 1151, 1152, 2533, 2595, 2863, 2975, 3023
StyI CCWWGG 1 cut(s) 2268
TaiI ACGT 4 cut(s) 706, 1799, 2146, 3933
TaqI TCGA 5 cut(s) 306, 1104, 1342, 2122, 3159
TatI WGTACW 5 cut(s) 1179, 2251, 3300, 3335, 3675
TfiI GAWTC 7 cut(s) 1519, 1953, 2491, 2735, 2780, 3251, 4064
Tru1I TTAA 6 cut(s) 299, 363, 1191, 1403, 4050, 4200
Tru9I TTAA 6 cut(s) 299, 363, 1191, 1403, 4050, 4200
TseFI GTSAC 3 cut(s) 1020, 1786, 2007
Tsp45I GTSAC 3 cut(s) 1020, 1786, 2007
TspGWI ACGGA 5 cut(s) 125, 1359, 2717, 3290, 4035
TspMI CCCGGG 1 cut(s) 1152
Van91I CCANNNNNTGG 2 cut(s) 494, 2963
VpaK11BI GGWCC 8 cut(s) 112, 241, 698, 947, 1148, 1699, 3128, 4011
XceI RCATGY 5 cut(s) 250, 2075, 2458, 2723, 4148
XcmI CCANNNNNNNNNTGG 8 cut(s) 440, 511, 1046, 1853, 2142, 2266, 2944, 3748
XhoI CTCGAG 1 cut(s) 2121
XmaI CCCGGG 1 cut(s) 1152
XmiI GTMKAC 1 cut(s) 3762
XspI CTAG 5 cut(s) 797, 1458, 1806, 2036, 2357
ZrmI AGTACT 2 cut(s) 2253, 3337
Zsp2I ATGCAT 2 cut(s) 3407, 3697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.