RLG00000029779

cwf21 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002497
Physical Location & Seq
Forward (+)
48930706 .. 48931424
719 bp
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UTR
Exon/CDS
Intron
RLM00000029779

Sequence Viewer

Length: 612 bp
ATGAAGCATAATAAGACTAAAAACGATGATACTGATGATTATGTTGCCAATGAAGATGCCGAGGGCAAACAACATGTGAAACCAAACATAAGGCATGACTCTGGGAATGAGTCTGACTTTGACAGCAGAATGGAAAGGAGAGGTAGCCAAGTTGAGCAGCAAAGGAATCAGCAGGTCAGCCGTAGGGTAGATCGAGATGATTTTGATGTGGAGAAGGCCAACAATGTTAGAAGTGGAAAAAGCATAAAATCATATGATAGTGATTCTATTTCTGACTATGAAAGATCCAGAAAAAGTAGAGGTGCAATAACAAAAAAAAGTAGAAGAAGTGGCAGGAATGATACTAATGATGATGATACTAATATTGGGAGGAAACCTGCAAGGGTTGAAGAGGGTGATGGAGGACGTGGAAGGCATAACAAGAGTGAACTGCAAAGAGGAAGGAAAAATGAAAGAGATGAAGAGGATCATGATTATAGAAGGCCCAGGGAAGGTCTGGAACAGGAACTTGGAAATAGAAAGCGTGGTACTAGAGAGGAAGAAGAGGCAAATGAGGATATTGAGAGGGATATACATATGGATTACAGGAGAGCAAAATATGATGGAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cwf21

Protein Analysis

204

Amino Acids

23.81

Weight (kDa)

6.35

Isoelectric Point (pI)

67.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 163, 385
AclWI GGATC 2 cut(s) 279, 474
AfaI GTAC 1 cut(s) 529
AfiI CCNNNNNNNGG 1 cut(s) 491
AflIII ACRYGT 1 cut(s) 73
AgsI TTSAA 1 cut(s) 389
AjiI CACGTC 1 cut(s) 407
AjnI CCWGG 1 cut(s) 485
AjuI GAANNNNNNNTTGG 2 cut(s) 492, 524
AlwI GGATC 2 cut(s) 279, 474
AoxI GGCC 2 cut(s) 216, 482
ApeKI GCWGC 1 cut(s) 157
AspS9I GGNCC 1 cut(s) 483
AsuHPI GGTGA 1 cut(s) 407
BbvI GCAGC 1 cut(s) 169
BccI CCATC 2 cut(s) 392, 596
BceAI ACGGC 1 cut(s) 165
BciT130I CCWGG 1 cut(s) 487
BfaI CTAG 2 cut(s) 531, 610
BfuAI ACCTGC 2 cut(s) 163, 385
BisI GCNGC 1 cut(s) 158
BlsI GCNGC 1 cut(s) 159
Bme1390I CCNGG 1 cut(s) 487
BmgBI CACGTC 1 cut(s) 407
BmgT120I GGNCC 1 cut(s) 483
BmrFI CCNGG 1 cut(s) 487
BmsI GCATC 1 cut(s) 46
BsaJI CCNNGG 3 cut(s) 60, 485, 486
Bsc4I CCNNNNNNNGG 1 cut(s) 491
BseBI CCWGG 1 cut(s) 487
BseDI CCNNGG 3 cut(s) 60, 485, 486
BseLI CCNNNNNNNGG 1 cut(s) 491
BseXI GCAGC 1 cut(s) 169
BshFI GGCC 2 cut(s) 218, 484
BslI CCNNNNNNNGG 1 cut(s) 491
BsnI GGCC 2 cut(s) 218, 484
Bsp143I GATC 3 cut(s) 190, 284, 466
BspANI GGCC 2 cut(s) 218, 484
BspHI TCATGA 1 cut(s) 469
BspMI ACCTGC 2 cut(s) 163, 385
BspPI GGATC 2 cut(s) 279, 474
BssECI CCNNGG 3 cut(s) 60, 485, 486
BssMI GATC 3 cut(s) 190, 284, 466
Bst2UI CCWGG 1 cut(s) 487
Bst6I CTCTTC 3 cut(s) 384, 456, 537
BstKTI GATC 3 cut(s) 193, 287, 469
BstMBI GATC 3 cut(s) 190, 284, 466
BstNI CCWGG 1 cut(s) 487
BstNSI RCATGY 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 485
BstV1I GCAGC 1 cut(s) 169
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
BsuRI GGCC 2 cut(s) 218, 484
BtrI CACGTC 1 cut(s) 407
BveI ACCTGC 2 cut(s) 163, 385
CciI TCATGA 1 cut(s) 469
Cfr13I GGNCC 1 cut(s) 483
Csp6I GTAC 1 cut(s) 528
CviAII CATG 3 cut(s) 74, 95, 470
CviJI RGCY 4 cut(s) 147, 180, 218, 484
CviKI_1 RGCY 4 cut(s) 147, 180, 218, 484
CviQI GTAC 1 cut(s) 528
DpnI GATC 3 cut(s) 192, 286, 468
DpnII GATC 3 cut(s) 190, 284, 466
Eam1104I CTCTTC 3 cut(s) 384, 456, 537
EarI CTCTTC 3 cut(s) 384, 456, 537
EcoRII CCWGG 1 cut(s) 485
FaeI CATG 3 cut(s) 77, 98, 473
FatI CATG 3 cut(s) 73, 94, 469
FauNDI CATATG 2 cut(s) 253, 576
Fnu4HI GCNGC 1 cut(s) 158
Fsp4HI GCNGC 1 cut(s) 158
FspBI CTAG 2 cut(s) 531, 610
GluI GCNGC 1 cut(s) 158
HaeIII GGCC 2 cut(s) 218, 484
Hin1II CATG 3 cut(s) 77, 98, 473
HinfI GANTC 5 cut(s) 98, 110, 166, 263, 606
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 1 cut(s) 428
Hpy188I TCNGA 2 cut(s) 115, 274
Hpy188III TCNNGA 4 cut(s) 194, 288, 470, 497
Hpy8I GTNNAC 1 cut(s) 428
HpyAV CCTTC 5 cut(s) 208, 405, 435, 474, 485
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 3 cut(s) 305, 380, 433
HpySE526I ACGT 1 cut(s) 406
Hsp92II CATG 3 cut(s) 77, 98, 473
Kzo9I GATC 3 cut(s) 190, 284, 466
Lsp1109I GCAGC 1 cut(s) 169
LweI GCATC 1 cut(s) 46
MaeI CTAG 2 cut(s) 531, 610
MaeII ACGT 1 cut(s) 406
MalI GATC 3 cut(s) 192, 286, 468
MboI GATC 3 cut(s) 190, 284, 466
MboII GAAGA 6 cut(s) 65, 336, 401, 473, 551, 554
MflI RGATCY 1 cut(s) 284
MlyI GAGTC 2 cut(s) 92, 119
MspR9I CCNGG 1 cut(s) 487
MvaI CCWGG 1 cut(s) 487
NdeI CATATG 2 cut(s) 253, 576
NdeII GATC 3 cut(s) 190, 284, 466
NlaIII CATG 3 cut(s) 77, 98, 473
NmeAIII GCCGAG 1 cut(s) 85
NspI RCATGY 1 cut(s) 77
PagI TCATGA 1 cut(s) 469
PasI CCCWGGG 1 cut(s) 486
PciI ACATGT 1 cut(s) 73
PfeI GAWTC 2 cut(s) 166, 263
PkrI GCNGC 1 cut(s) 159
PleI GAGTC 2 cut(s) 92, 118
PpsI GAGTC 2 cut(s) 92, 118
PscI ACATGT 1 cut(s) 73
Psp6I CCWGG 1 cut(s) 485
PspGI CCWGG 1 cut(s) 485
PspPI GGNCC 1 cut(s) 483
PsuI RGATCY 1 cut(s) 284
RsaI GTAC 1 cut(s) 529
RsaNI GTAC 1 cut(s) 528
SatI GCNGC 1 cut(s) 158
Sau3AI GATC 3 cut(s) 190, 284, 466
Sau96I GGNCC 1 cut(s) 483
SchI GAGTC 2 cut(s) 92, 119
ScrFI CCNGG 1 cut(s) 487
SetI ASST 6 cut(s) 145, 177, 304, 379, 409, 496
SfaNI GCATC 1 cut(s) 46
SspI AATATT 1 cut(s) 364
SspMI CTAG 2 cut(s) 531, 610
StyD4I CCNGG 1 cut(s) 485
TaiI ACGT 1 cut(s) 409
TaqI TCGA 1 cut(s) 193
TfiI GAWTC 2 cut(s) 166, 263
TseI GCWGC 1 cut(s) 157
TspDTI ATGAA 5 cut(s) 17, 66, 294, 465, 474
XceI RCATGY 1 cut(s) 77
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XspI CTAG 2 cut(s) 531, 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.