Rorug06G0395300

cwf21 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002497
Physical Location & Seq
Forward (+)
54058359 .. 54065583
7225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0395300.1

Sequence Viewer

Length: 4356 bp
ATGGAGAGTAGTAGTGGAGATGTATATAGAGTGAGCAGTGCTCGTCTGAGCAGTTCAAACATTTGGAGGAACAGTACCATGGATGTTTTCTCCAAGTCTTCACACGATGAAGACGATGAAGAAGCGCTGAAATGGGCCGCCATTGAAAAACTGCCAACTTACTTGCGTATAAGGAGAGGCATTCTCACCGAGGAAGAAGGCGAAGCGAGAGAGATTGACATCAAGAATCTTGGATTGTTGGAAAGGAAGAATGTGTTGGAGAGGCTGGTGAAAGTTGCAGATGAAGATAATGAGAGGTTCTTGATGAAGCTCAAGGACCGGATTAATAGGGTTGGACTTGATATTCCGACGATTGAAGTCCGGTTTGAGCATTTGAATGTTGAAGCAGAAGCTTATGTAGGAGGGAGGGCGCTGCCTACACTCTATAACTTTTCTGTTAATATCTTGGAGGGATTCCTGAGTTTCTGTCGCATTATTCCAACTAGAAAGCATCCATTACCAATCCTTGATGATGTTAGTGGAATTATCAAACCAAAAAGAATGACACTGCTTTTAGGCCCCCCAAACTCTGGAAAAACCACATTGCTTTTGGCTTTGGCTGGTAAACTTGCTAAAGATCTAAAGTCTTCTGGGAGAGTTGCATATAACGGACATGGGATGGAAGAGTTTGTACCGGAGAGGACATCAGCTTATATTAGTCAGTATGATCTCCACATAGGGGAAATGACAGTAAGAGAAACACTGGCCTTTTCAGCAAGGTGTCAAGGGGTTGGAGGACGTTATGAAATGTTGGCAGAGTTATCAAGGAGAGAAAAGGCAGCAAATATTATGCCAGAGAAAGATCTTGATATCTACATGAAGGCTGCATCATTAGAAGGGCAGGAGGCCAATGTAGTTACAGATTACATACTCAAGATTCTGGGACTTGAAGTTTGTGCTGACACCATGGTGGGGGATGAAATGTTCCGAGGTATATCTGGCGGACAAAAGAAGCGAGTCACAACAGGGGAAATGCTGGTTGGACCAGCAAGAGCACTTTTTATGGATGAGATATCAACTGGTTTAGACAGTTCAACAACATTTCAGATAGTGAATTCCCTCAGACAAACTATCCACATCCTCAATGGAACTGCTCTTATATCTCTTCTCCAGCCAGCACCGGAAACATATGGCCTGTTTGATGACATAATTCTTCTGTCAGATGGGCAAATTGTATACCAAGGTCCCCGCGAAAATGTGCTCGAGTTCTTTGAGTATATGGGCTTCAAATGTCCAGAGAGGAAAGGAGTTGCTGACTTCCTTCAAGAAGTGACTTCAAAGAAGGATCAGGAGCAGTACTGGATACAGAAAGAAGAGCCTTATAATTTTATATCTTCCAAGGAATTTGCTGAAGCATTTCAGTCATTTCATATTGGTCGAAAACTAGGTGATGATCTTGCTACTCCATTTGACAAGTCCAAAGGTCACCCTGCTGCTTTAACAACTAAGAAGTATGGTGTTAGCAAGAGGGAACTGCTCAAAGCCTGCATTGCTAGAGAATATTTGCTGATGAAGAGGAATTCGTTTGTGTACATTTTCAAAATGACCCAGCTCACTCTATTGGCCTTTATATCAATGACACTATTTTTCCGTACTAAGATGCACCGGGATACAGTTGAGGATGGTGGGATTTACATGGGTGCTATGTTCTTCACAGTCATCATAATTATGTTCAATGGGTTCTCCGAGCTTGCATTGACTATCATGAAACTTCCTGTATTTTTCAAGCAAAGAGACCTTCTGTTCTATCCAGCTTGGGCATATTCCTTACCAGCATGGATCCTTAAGATTCCCATCACCTTTGTAGAAGTTGCCATTTGGGTGTTCATGACATACTATGTAATTGGTTTTGATCCAAACCCCGGAAGGTTTTTCAAGCAATATCTTTTGCTCCTATGCATTAACCAGATGGCATCCGGATTATTCCGATTTATGGGGGCATTAGGGAGGAATATAATTGTTGCCAACACATTTGGTTCATTTGCTCTACTTGCAGTTCTGGTTATGGGTGGCTTTATCTTGTCCCGAGAGAATGTGCAGGCGTGGTGGTTATGGGGTTATTGGATCTCACCAATGATGTATGGCCAGAATGCTATAGCTGTCAATGAATTTCTTGGAGACAGTTGGAGTCATATTCCTCCTGACTCAACAGAGTCGTTAGGGATCATGGTCTTGAAGTCTCGTGGGGTTTTCGTACAAGCATATTGGTACTGGATTGGAGTAGGAGCTACAATTGGATATATATTTCTGTTTAATTTCTTCTACACTGTGGCCTTGCATTATCTTGATCCGTTTGGGAAACCTCAGGCTGTACTCTCCAAAGAAGCTTTAGCTGATAAAAACGCTGCTAGAGCTGGAGGAAACATAGAGCTATCATCAAGAGGAAAGAACACTTCAGATGCAAAGAATGATAGTCGAAGAAGTATCTCATCTAGAACACTGTCTGCTAGAGTGGGCAGCTTTAATGAAGCCAATGAAAACAGGAAGAGGGGAATGGTTCTTCCTTTTGAACCCCTTTGGATTACATTTAATGAAATCAAATATTCTGTGGACATGCCCGAGGAAATGAAAACTCAAGGAATAACTGAGGAACGGTTACCACTTCTAAAGGGTGTGACTGGTGCTTTTAGGCCTGGAGTTTTAACAGCTCTAATGGGCATAAGTGGTGCTGGAAAGACTACTCTAATGGATGTCTTGGCAGGCAGAAAAACTGGAGGATATATTGAGGGGGACATAACCATTTCTGGGTATCCAAAAAAGCAAGAAACATTTGCTCGTATATCAGGATATTGTGAGCAAACCGATATACACTCTCCTCATGTTACAGTATATGAGTCTTTGGTTTATTCTGCATGGCTCCGGTTGCCCCCTGAGGTTGATTCCGCAACTAGAAAGATGTTTGTTGAGGAAGTCATGGAGCTTGTGGAACTGACGTCGATAAGGGAGTCTCTTGTGGGATTGCCAGGAGTAAATGGTCTCTCAACCGAGCAGCGTAAAAGGCTAACCATTGCTGTAGAACTTGTTGCTAACCCTTCCATAATATTTATGGATGAGCCAACTTCTGGTCTTGATGCAAGGGCAGCAGCTATTGTAATGAGAACAGTGAGGAATACTGTGGACACAGGGCGAACTGTAGTCTGCACTATCCACCAGCCAAGCATCGACATTTTTGATGCTTTTGATGAGCTGTTTCTATTGAAAAGGGGAGGTGAAGAGATATATGTTGGCCCTCTAGGCCGACATTCTTCCCAGTTGATAAAGTATTTTGAGGAAATTGATGGGGTTCCCAAAATCAAGGATGGTTACAATCCAGCAACTTGGATGTTGGAGGTTACTTCAGCAGCACAGGAAGTAGTTCTCGGTATTAACTTTACCGATATATACAGGAGCTCAGAGCTATATAGGAGAAACAAGGACCTGATCAAGGAACTGAGTACTCCTCCGCAAGGCACAAAAGACTTGTACTTCTCTACTAAATACTCTCAGTCTTTCTACTATCAGTGTATGGCTTGTTTATGGAAACAGCATCTATCATACTGGCGAAACCCACCATACAGTGCAGTGAGACTCCTATTCACCACTTTCATAGCCTTAATGTTTGGAACAATATTCTGGGATCTTGGCTCCAAAAGGAAAAACCAACAAGATCTATTCAATGCTATGGGTTCCATGTACGCCGCTGTTCTCTTCATTGGAGTACAAAATGCCACATCGGTGCAGCCAGTTGTGGCTATCGAGAGGACAGTCTTTTACAGAGAAAGGGCTGCTGGAATGTACTCAGCATTGCCATATGCCTTCGGACAGGTTGTGATTGAGCTTCCATACATCTTTGTCCAGACTGTCATATATGGAGTTATAGTATACGCCATGATTGGATTTGACTGGACAGTGAGCAAGTTCCTTTGGTATCTCTTCTTCATGTACTTCACTTTCTTATACTTTACATTCTATGGGATGATGACCGTGGCTGTTACACCCAACCACAACATTGCCGCCATAGTGTCCTCTGCCTTCTATGCAATATGGAACCTTTTCTCAGGATTCATTGTTCCCCGAACAAGAATGCCAATTTGGTGGAGATGGTACTATTGGATCTGCCCGGTCTCATATACATTGTATGGACTTGTTGCTTCACAGTTTGGAGACATAAAAGAAGTAATGGATTCAGGTGAAAGTGTGGAAGACTTTGTGAGGAACTACTTTGGTTACAGACACGACTTTGTAGGTATTGTTGCAGTTGTGCTTGTTGGGATTTCAGTGCTTTTCGGGTTCACCTTTGCCTTCTCGATCAAGGCATTCAACTTCCAAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cwf21

Protein Analysis

1451

Amino Acids

164.42

Weight (kDa)

7.03

Isoelectric Point (pI)

40.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 94 - 144 1.1e-06 ABC-transporter N-terminal
ABC_tran PF00005 169 - 350 6.3e-14 ABC transporter
ABC2_membrane PF01061 505 - 717 1.2e-47 ABC-2 type transporter
PDR_assoc PF08370 722 - 784 1.1e-27 Plant PDR ABC transporter associated
ABC_tran PF00005 882 - 1034 5.8e-20 ABC transporter
ABC2_membrane PF01061 1179 - 1393 2e-64 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1362
AatII GACGTC 1 cut(s) 2975
AccB7I CCANNNNNTGG 2 cut(s) 569, 3101
AccI GTMKAC 2 cut(s) 1215, 3900
AccII CGCG 1 cut(s) 1230
AccIII TCCGGA 1 cut(s) 1955
AciI CCGC 7 cut(s) 138, 981, 1228, 2922, 3482, 3717, 4032
AclWI GGATC 9 cut(s) 1332, 1813, 1826, 1886, 2111, 2210, 2321, 3663, 4139
AcoI YGGCCR 1 cut(s) 2122
AcsI RAATTY 4 cut(s) 1093, 1382, 1558, 2147
AcuI CTGAAG 3 cut(s) 1410, 2418, 3360
AcyI GRCGYC 1 cut(s) 2972
AfeI AGCGCT 1 cut(s) 126
AfiI CCNNNNNNNGG 9 cut(s) 569, 718, 951, 1901, 1972, 2784, 3101, 3463, 3485
AflII CTTAAG 1 cut(s) 1823
AjnI CCWGG 2 cut(s) 2671, 3001
AjuI GAANNNNNNNTTGG 6 cut(s) 239, 271, 1002, 1034, 4093, 4125
AleI CACNNNNGTG 2 cut(s) 947, 3752
AloI GAACNNNNNNTCC 4 cut(s) 947, 979, 4071, 4103
Alw21I GWGCWC 4 cut(s) 43, 1036, 1242, 3431
Alw26I GTCTC 8 cut(s) 1767, 2151, 2223, 2991, 3020, 3598, 4147, 4176
AlwI GGATC 9 cut(s) 1332, 1813, 1826, 1886, 2111, 2210, 2321, 3663, 4139
Ama87I CYCGRG 3 cut(s) 1241, 2064, 2597
Aor13HI TCCGGA 1 cut(s) 1955
Aor51HI AGCGCT 1 cut(s) 126
ApoI RAATTY 4 cut(s) 1093, 1382, 1558, 2147
ArsI GACNNNNNNTTYG 2 cut(s) 915, 947
AseI ATTAAT 1 cut(s) 324
Asp700I GAANNNNTTC 3 cut(s) 1395, 3393, 4070
AspLEI GCGC 2 cut(s) 127, 412
AspS9I GGNCC 7 cut(s) 135, 316, 557, 1022, 1223, 3266, 3454
AsuC2I CCSGG 3 cut(s) 1646, 1902, 4139
AvaI CYCGRG 3 cut(s) 1241, 2064, 2597
AvaII GGWCC 4 cut(s) 316, 1022, 1223, 3454
AxyI CCTNAGG 2 cut(s) 2343, 2910
BalI TGGCCA 1 cut(s) 2124
BamHI GGATCC 1 cut(s) 1818
BanII GRGCYC 1 cut(s) 3431
BarI GAAGNNNNNNTAC 6 cut(s) 654, 686, 1247, 1279, 3938, 3970
BauI CACGAG 1 cut(s) 2220
BbsI GAAGAC 4 cut(s) 90, 117, 618, 4227
Bbv12I GWGCWC 4 cut(s) 43, 1036, 1242, 3431
BccI CCATC 8 cut(s) 652, 1196, 1655, 1841, 1942, 3311, 3332, 4113
BciT130I CCWGG 2 cut(s) 2673, 3003
BciVI GTATCC 3 cut(s) 1335, 1642, 2799
BclI TGATCA 1 cut(s) 3459
BcnI CCSGG 3 cut(s) 1646, 1902, 4139
BcoDI GTCTC 8 cut(s) 1767, 2151, 2223, 2991, 3020, 3598, 4147, 4176
BfaI CTAG 8 cut(s) 483, 1424, 1533, 2388, 2472, 2487, 2928, 3272
BfmI CTRYAG 3 cut(s) 2133, 3051, 3171
BfoI RGCGCY 2 cut(s) 128, 413
BfrI CTTAAG 1 cut(s) 1823
BfuI GTATCC 3 cut(s) 1335, 1642, 2799
BglI GCCNNNNNGGC 1 cut(s) 3273
BglII AGATCT 3 cut(s) 616, 841, 3685
BmcAI AGTACT 2 cut(s) 1337, 3475
Bme1390I CCNGG 5 cut(s) 1646, 1902, 2673, 3003, 4139
Bme18I GGWCC 4 cut(s) 316, 1022, 1223, 3454
BmeT110I CYCGRG 3 cut(s) 1241, 2064, 2597
BmgT120I GGNCC 7 cut(s) 135, 316, 557, 1022, 1223, 3266, 3454
BmiI GGNNCC 8 cut(s) 559, 1225, 1820, 2897, 3324, 3664, 3706, 4067
BmrFI CCNGG 5 cut(s) 1646, 1902, 2673, 3003, 4139
BmrI ACTGGG 1 cut(s) 3283
BmsI GCATC 9 cut(s) 499, 875, 1629, 1961, 2428, 3100, 3202, 3207, 3574
BmuI ACTGGG 1 cut(s) 3283
BpiI GAAGAC 4 cut(s) 90, 117, 618, 4227
BplI GAGNNNNNCTC 6 cut(s) 25, 57, 168, 200, 3463, 3495
BpmI CTGGAG 4 cut(s) 1133, 2415, 2694, 2772
BpuEI CTTGAG 3 cut(s) 296, 896, 2598
BpuMI CCSGG 3 cut(s) 1646, 1902, 4139
BsaBI GATNNNNATC 2 cut(s) 218, 1832
BsaHI GRCGYC 1 cut(s) 2972
BsaI GGTCTC 3 cut(s) 1767, 3020, 4147
BsaJI CCNNGG 9 cut(s) 78, 189, 945, 967, 1219, 1377, 1900, 2598, 4002
BsaWI WCCGGW 6 cut(s) 318, 360, 673, 1159, 1955, 2898
BsaXI ACNNNNNCTCC 4 cut(s) 58, 88, 3882, 3912
Bsc4I CCNNNNNNNGG 9 cut(s) 569, 718, 951, 1901, 1972, 2784, 3101, 3463, 3485
Bse21I CCTNAGG 2 cut(s) 2343, 2910
Bse3DI GCAATG 5 cut(s) 581, 1527, 3045, 3821, 4026
Bse8I GATNNNNATC 2 cut(s) 218, 1832
BseAI TCCGGA 1 cut(s) 1955
BseBI CCWGG 2 cut(s) 2673, 3003
BseDI CCNNGG 9 cut(s) 78, 189, 945, 967, 1219, 1377, 1900, 2598, 4002
BseJI GATNNNNATC 2 cut(s) 218, 1832
BseLI CCNNNNNNNGG 9 cut(s) 569, 718, 951, 1901, 1972, 2784, 3101, 3463, 3485
BseMI GCAATG 5 cut(s) 581, 1527, 3045, 3821, 4026
BseRI GAGGAG 2 cut(s) 2844, 3468
BseYI CCCAGC 1 cut(s) 1587
BsgI GTGCAG 4 cut(s) 2096, 3163, 3618, 3776
Bsh1236I CGCG 1 cut(s) 1230
BsiHKAI GWGCWC 4 cut(s) 43, 1036, 1242, 3431
BsiHKCI CYCGRG 3 cut(s) 1241, 2064, 2597
BsiSI CCGG 9 cut(s) 319, 361, 674, 1160, 1645, 1902, 1956, 2899, 4139
BslFI GGGAC 4 cut(s) 936, 1209, 2047, 2783
BslI CCNNNNNNNGG 9 cut(s) 569, 718, 951, 1901, 1972, 2784, 3101, 3463, 3485
BsmAI GTCTC 8 cut(s) 1767, 2151, 2223, 2991, 3020, 3598, 4147, 4176
BsmFI GGGAC 4 cut(s) 936, 1209, 2047, 2783
BsmI GAATGC 4 cut(s) 180, 2134, 4107, 4334
Bso31I GGTCTC 3 cut(s) 1767, 3020, 4147
BsoBI CYCGRG 3 cut(s) 1241, 2064, 2597
Bsp1286I GDGCHC 4 cut(s) 43, 1036, 1242, 3431
Bsp13I TCCGGA 1 cut(s) 1955
Bsp1407I TGTACA 1 cut(s) 1569
Bsp19I CCATGG 2 cut(s) 78, 945
BspACI CCGC 7 cut(s) 138, 981, 1228, 2922, 3482, 3717, 4032
BspEI TCCGGA 1 cut(s) 1955
BspFNI CGCG 1 cut(s) 1230
BspHI TCATGA 2 cut(s) 1743, 1866
BspLI GGNNCC 8 cut(s) 559, 1225, 1820, 2897, 3324, 3664, 3706, 4067
BspPI GGATC 9 cut(s) 1332, 1813, 1826, 1886, 2111, 2210, 2321, 3663, 4139
BspQI GCTCTTC 1 cut(s) 1347
BspTI CTTAAG 1 cut(s) 1823
BspTNI GGTCTC 3 cut(s) 1767, 3020, 4147
BsrDI GCAATG 5 cut(s) 581, 1527, 3045, 3821, 4026
BsrGI TGTACA 1 cut(s) 1569
BssECI CCNNGG 9 cut(s) 78, 189, 945, 967, 1219, 1377, 1900, 2598, 4002
BssNAI GTATAC 2 cut(s) 1216, 3901
BssNI GRCGYC 1 cut(s) 2972
BssSI CACGAG 1 cut(s) 2220
BssT1I CCWWGG 4 cut(s) 78, 945, 1219, 1377
Bst1107I GTATAC 2 cut(s) 1216, 3901
Bst2BI CACGAG 1 cut(s) 2220
Bst2UI CCWGG 2 cut(s) 2673, 3003
Bst6I CTCTTC 8 cut(s) 657, 1149, 1347, 1547, 2519, 3246, 3731, 3956
BstACI GRCGYC 1 cut(s) 2972
BstAFI CTTAAG 1 cut(s) 1823
BstAUI TGTACA 1 cut(s) 1569
BstC8I GCNNGC 5 cut(s) 1155, 1525, 1731, 2079, 2740
BstDSI CCRYGG 3 cut(s) 78, 945, 4002
BstEII GGTNACC 2 cut(s) 1463, 2634
BstENI CCTNNNNNAGG 2 cut(s) 3461, 3483
BstFNI CGCG 1 cut(s) 1230
BstH2I RGCGCY 2 cut(s) 128, 413
BstHHI GCGC 2 cut(s) 127, 412
BstMAI GTCTC 8 cut(s) 1767, 2151, 2223, 2991, 3020, 3598, 4147, 4176
BstMWI GCNNNNNNNGC 9 cut(s) 752, 1529, 2030, 2390, 2902, 3037, 3119, 3273, 4055
BstNI CCWGG 2 cut(s) 2673, 3003
BstNSI RCATGY 1 cut(s) 2596
BstPI GGTNACC 2 cut(s) 1463, 2634
BstSCI CCNGG 5 cut(s) 1644, 1900, 2671, 3001, 4137
BstSFI CTRYAG 3 cut(s) 2133, 3051, 3171
BstUI CGCG 1 cut(s) 1230
BstV2I GAAGAC 4 cut(s) 90, 117, 618, 4227
BstX2I RGATCY 7 cut(s) 616, 841, 1818, 2103, 3655, 3685, 4131
BstXI CCANNNNNNTGG 2 cut(s) 3357, 4113
BstYI RGATCY 7 cut(s) 616, 841, 1818, 2103, 3655, 3685, 4131
BstZ17I GTATAC 2 cut(s) 1216, 3901
Bsu36I CCTNAGG 2 cut(s) 2343, 2910
BsuI GTATCC 3 cut(s) 1335, 1642, 2799
BtgI CCRYGG 3 cut(s) 78, 945, 4002
BtsI GCAGTG 3 cut(s) 43, 545, 3606
Cac8I GCNNGC 5 cut(s) 1155, 1525, 1731, 2079, 2740
CciI TCATGA 2 cut(s) 1743, 1866
CfoI GCGC 2 cut(s) 127, 412
Cfr13I GGNCC 7 cut(s) 135, 316, 557, 1022, 1223, 3266, 3454
EaeI YGGCCR 1 cut(s) 2122
Eam1104I CTCTTC 8 cut(s) 657, 1149, 1347, 1547, 2519, 3246, 3731, 3956
EarI CTCTTC 8 cut(s) 657, 1149, 1347, 1547, 2519, 3246, 3731, 3956
EciI GGCGGA 1 cut(s) 996
Ecl136II GAGCTC 1 cut(s) 3429
Eco130I CCWWGG 4 cut(s) 78, 945, 1219, 1377
Eco147I AGGCCT 1 cut(s) 2671
Eco24I GRGCYC 1 cut(s) 3431
Eco31I GGTCTC 3 cut(s) 1767, 3020, 4147
Eco32I GATATC 2 cut(s) 850, 1053
Eco47I GGWCC 4 cut(s) 316, 1022, 1223, 3454
Eco47III AGCGCT 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 3429
Eco57I CTGAAG 3 cut(s) 1410, 2418, 3360
Eco81I CCTNAGG 2 cut(s) 2343, 2910
Eco88I CYCGRG 3 cut(s) 1241, 2064, 2597
Eco91I GGTNACC 2 cut(s) 1463, 2634
EcoICRI GAGCTC 1 cut(s) 3429
EcoNI CCTNNNNNAGG 2 cut(s) 3461, 3483
EcoO109I RGGNCCY 3 cut(s) 557, 1223, 3454
EcoO65I GGTNACC 2 cut(s) 1463, 2634
EcoRI GAATTC 2 cut(s) 1093, 1558
EcoRII CCWGG 2 cut(s) 2671, 3001
EcoRV GATATC 2 cut(s) 850, 1053
EcoT14I CCWWGG 4 cut(s) 78, 945, 1219, 1377
EcoT22I ATGCAT 1 cut(s) 1940
EcoT38I GRGCYC 1 cut(s) 3431
ErhI CCWWGG 4 cut(s) 78, 945, 1219, 1377
FaqI GGGAC 4 cut(s) 936, 1209, 2047, 2783
FauI CCCGC 1 cut(s) 1235
FauNDI CATATG 2 cut(s) 1168, 3829
FbaI TGATCA 1 cut(s) 3459
FblI GTMKAC 2 cut(s) 1215, 3900
FriOI GRGCYC 1 cut(s) 3431
FspBI CTAG 8 cut(s) 483, 1424, 1533, 2388, 2472, 2487, 2928, 3272
GlaI GCGC 2 cut(s) 126, 411
GsaI CCCAGC 1 cut(s) 1591
GsuI CTGGAG 4 cut(s) 1133, 2415, 2694, 2772
HaeII RGCGCY 2 cut(s) 128, 413
HapII CCGG 9 cut(s) 319, 361, 674, 1160, 1645, 1902, 1956, 2899, 4139
HhaI GCGC 2 cut(s) 127, 412
Hin1I GRCGYC 1 cut(s) 2972
Hin6I GCGC 2 cut(s) 125, 410
HinP1I GCGC 2 cut(s) 125, 410
HindIII AAGCTT 2 cut(s) 390, 2364
HpaII CCGG 9 cut(s) 319, 361, 674, 1160, 1645, 1902, 1956, 2899, 4139
Hpy166II GTNNAC 7 cut(s) 605, 1216, 1570, 2590, 3157, 3901, 4311
Hpy8I GTNNAC 7 cut(s) 605, 1216, 1570, 2590, 3157, 3901, 4311
Hpy99I CGWCG 2 cut(s) 352, 2977
HpyCH4IV ACGT 2 cut(s) 778, 2972
HpyF10VI GCNNNNNNNGC 9 cut(s) 752, 1529, 2030, 2390, 2902, 3037, 3119, 3273, 4055
HpySE526I ACGT 2 cut(s) 778, 2972
Hsp92I GRCGYC 1 cut(s) 2972
HspAI GCGC 2 cut(s) 125, 410
Kpn2I TCCGGA 1 cut(s) 1955
Ksp22I TGATCA 1 cut(s) 3459
LguI GCTCTTC 1 cut(s) 1347
LmnI GCTCC 7 cut(s) 1330, 1935, 2264, 2901, 2956, 3426, 3668
LweI GCATC 9 cut(s) 499, 875, 1629, 1961, 2428, 3100, 3202, 3207, 3574
MaeI CTAG 8 cut(s) 483, 1424, 1533, 2388, 2472, 2487, 2928, 3272
MaeII ACGT 2 cut(s) 778, 2972
MfeI CAATTG 1 cut(s) 2271
MflI RGATCY 7 cut(s) 616, 841, 1818, 2103, 3655, 3685, 4131
MhlI GDGCHC 4 cut(s) 43, 1036, 1242, 3431
MlsI TGGCCA 1 cut(s) 2124
MluNI TGGCCA 1 cut(s) 2124
MlyI GAGTC 7 cut(s) 1005, 2176, 2177, 2201, 2882, 2993, 3600
MmeI TCCRAC 9 cut(s) 219, 237, 313, 371, 503, 751, 1000, 2144, 3345
Mox20I TGGCCA 1 cut(s) 2124
Mph1103I ATGCAT 1 cut(s) 1940
MroI TCCGGA 1 cut(s) 1955
MroXI GAANNNNTTC 3 cut(s) 1395, 3393, 4070
MscI TGGCCA 1 cut(s) 2124
MslI CAYNNNNRTG 5 cut(s) 375, 947, 1706, 1859, 3752
Msp20I TGGCCA 1 cut(s) 2124
MspA1I CMGCKG 1 cut(s) 3719
MspCI CTTAAG 1 cut(s) 1823
MspI CCGG 9 cut(s) 319, 361, 674, 1160, 1645, 1902, 1956, 2899, 4139
MspR9I CCNGG 5 cut(s) 1646, 1902, 2673, 3003, 4139
MunI CAATTG 1 cut(s) 2271
Mva1269I GAATGC 4 cut(s) 180, 2134, 4107, 4334
MvaI CCWGG 2 cut(s) 2673, 3003
MvnI CGCG 1 cut(s) 1230
MwoI GCNNNNNNNGC 9 cut(s) 752, 1529, 2030, 2390, 2902, 3037, 3119, 3273, 4055
NciI CCSGG 3 cut(s) 1646, 1902, 4139
NcoI CCATGG 2 cut(s) 78, 945
NdeI CATATG 2 cut(s) 1168, 3829
NlaIV GGNNCC 8 cut(s) 559, 1225, 1820, 2897, 3324, 3664, 3706, 4067
NmuCI GTSAC 4 cut(s) 997, 1309, 1463, 2653
NsiI ATGCAT 1 cut(s) 1940
NspI RCATGY 1 cut(s) 2596
OliI CACNNNNGTG 2 cut(s) 947, 3752
PaeR7I CTCGAG 1 cut(s) 1241
PagI TCATGA 2 cut(s) 1743, 1866
PceI AGGCCT 1 cut(s) 2671
PciSI GCTCTTC 1 cut(s) 1347
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PctI GAATGC 4 cut(s) 180, 2134, 4107, 4334
PdmI GAANNNNTTC 3 cut(s) 1395, 3393, 4070
PfeI GAWTC 7 cut(s) 226, 453, 916, 1828, 2918, 4080, 4202
PflMI CCANNNNNTGG 2 cut(s) 569, 3101
PleI GAGTC 7 cut(s) 1004, 2175, 2177, 2200, 2881, 2992, 3600
PpsI GAGTC 7 cut(s) 1004, 2175, 2177, 2200, 2881, 2992, 3600
PpuMI RGGWCCY 2 cut(s) 1223, 3454
PshBI ATTAAT 1 cut(s) 324
PsiI TTATAA 1 cut(s) 1362
Psp124BI GAGCTC 1 cut(s) 3431
Psp5II RGGWCCY 2 cut(s) 1223, 3454
Psp6I CCWGG 2 cut(s) 2671, 3001
PspEI GGTNACC 2 cut(s) 1463, 2634
PspFI CCCAGC 1 cut(s) 1587
PspGI CCWGG 2 cut(s) 2671, 3001
PspN4I GGNNCC 8 cut(s) 559, 1225, 1820, 2897, 3324, 3664, 3706, 4067
PspPI GGNCC 7 cut(s) 135, 316, 557, 1022, 1223, 3266, 3454
PspPPI RGGWCCY 2 cut(s) 1223, 3454
PspXI VCTCGAGB 1 cut(s) 1241
PsuI RGATCY 7 cut(s) 616, 841, 1818, 2103, 3655, 3685, 4131
RseI CAYNNNNRTG 5 cut(s) 375, 947, 1706, 1859, 3752
SacI GAGCTC 1 cut(s) 3431
SapI GCTCTTC 1 cut(s) 1347
Sau96I GGNCC 7 cut(s) 135, 316, 557, 1022, 1223, 3266, 3454
ScaI AGTACT 2 cut(s) 1337, 3475
SchI GAGTC 7 cut(s) 1005, 2176, 2177, 2201, 2882, 2993, 3600
ScrFI CCNGG 5 cut(s) 1646, 1902, 2673, 3003, 4139
SduI GDGCHC 4 cut(s) 43, 1036, 1242, 3431
SfaNI GCATC 9 cut(s) 499, 875, 1629, 1961, 2428, 3100, 3202, 3207, 3574
SfcI CTRYAG 3 cut(s) 2133, 3051, 3171
SfiI GGCCNNNNNGGCC 1 cut(s) 3273
Sfr274I CTCGAG 1 cut(s) 1241
SinI GGWCC 4 cut(s) 316, 1022, 1223, 3454
SlaI CTCGAG 1 cut(s) 1241
SmiMI CAYNNNNRTG 5 cut(s) 375, 947, 1706, 1859, 3752
SmlI CTYRAG 5 cut(s) 311, 911, 1241, 1823, 2613
SmoI CTYRAG 5 cut(s) 311, 911, 1241, 1823, 2613
SseBI AGGCCT 1 cut(s) 2671
SsiI CCGC 7 cut(s) 138, 981, 1228, 2922, 3482, 3717, 4032
SspI AATATT 5 cut(s) 826, 1541, 2582, 3081, 3648
SspMI CTAG 8 cut(s) 483, 1424, 1533, 2388, 2472, 2487, 2928, 3272
SstI GAGCTC 1 cut(s) 3431
StuI AGGCCT 1 cut(s) 2671
StyD4I CCNGG 5 cut(s) 1644, 1900, 2671, 3001, 4137
StyI CCWWGG 4 cut(s) 78, 945, 1219, 1377
TaiI ACGT 2 cut(s) 781, 2975
TaqI TCGA 7 cut(s) 1242, 1417, 2455, 2975, 3201, 3774, 4325
TatI WGTACW 8 cut(s) 1335, 1569, 2350, 3473, 3501, 3736, 3813, 3960
TauI GCSGC 3 cut(s) 140, 3719, 4034
TfiI GAWTC 7 cut(s) 226, 453, 916, 1828, 2918, 4080, 4202
TseFI GTSAC 4 cut(s) 997, 1309, 1463, 2653
Tsp45I GTSAC 4 cut(s) 997, 1309, 1463, 2653
TspGWI ACGGA 3 cut(s) 663, 1619, 2319
Van91I CCANNNNNTGG 2 cut(s) 569, 3101
Vha464I CTTAAG 1 cut(s) 1823
VpaK11BI GGWCC 4 cut(s) 316, 1022, 1223, 3454
VspI ATTAAT 1 cut(s) 324
XagI CCTNNNNNAGG 2 cut(s) 3461, 3483
XapI RAATTY 4 cut(s) 1093, 1382, 1558, 2147
XbaI TCTAGA 1 cut(s) 2471
XceI RCATGY 1 cut(s) 2596
XcmI CCANNNNNNNNNTGG 3 cut(s) 586, 1121, 3082
XhoI CTCGAG 1 cut(s) 1241
XmiI GTMKAC 2 cut(s) 1215, 3900
XmnI GAANNNNTTC 3 cut(s) 1395, 3393, 4070
XspI CTAG 8 cut(s) 483, 1424, 1533, 2388, 2472, 2487, 2928, 3272
ZraI GACGTC 1 cut(s) 2973
ZrmI AGTACT 2 cut(s) 1337, 3475
Zsp2I ATGCAT 1 cut(s) 1940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.