Rorug06G0395200

cwf21 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002497
Physical Location & Seq
Forward (+)
54044565 .. 54045305
741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0395200.1

Sequence Viewer

Length: 606 bp
ATGGCCCAGACCATTGGTGCAACCAACAGATCCAACCCGTCACCCTTCGAGGCCCTAGCTGGGCTCTTTTGGGTCTGCATTAGCAAGGGGATGAGAAATGAGCTTATCAACATGTCTATATGCATGGATATGAGAAAGGTTTTAGGTTGGGACAAAGGCTTCTTTGGAAATTGCATGGTATATAACAAGGTACATCAAGATGGAATCCCACAAAACAACTTATCAGAAGCTGTGAGTTCTATAGGTGAGTTGGTAGCAAAAATGGACAACGAGGGTATCATGGATTTGATCGAGTGGCTCGAATGTACCGATGACCAATGTCCTTCGCTGATGAACGATTCAGATCTCATTTGTGCTAGCTTAGAGGCTGTAGACCCGTTTTGGACAGTTTTCGAAGATGGGATTGCACCCATTCGCATGTCGTATTATGTAGAGCCGGTGTTGGGGTTAGGGCAGGTTCTAATTTTTCCGGCAAAGCCGGAAGACGGTGAATTGAGTAGGGTGGTAATAATTACACTTTCACATGACGAGATGGTCAAGTTATGTGAAGACGATCTTATTTTACTCCTATCTCCGAATATCTTTATGAACGTGAATAAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cwf21

Protein Analysis

201

Amino Acids

22.4

Weight (kDa)

4.28

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 11 - 183 3.5e-08 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 533
Acc36I ACCTGC 1 cut(s) 445
AccI GTMKAC 1 cut(s) 372
AclWI GGATC 1 cut(s) 24
AfaI GTAC 2 cut(s) 192, 307
AfiI CCNNNNNNNGG 3 cut(s) 60, 443, 485
AflIII ACRYGT 1 cut(s) 111
AluBI AGCT 4 cut(s) 59, 103, 230, 360
AluI AGCT 4 cut(s) 59, 103, 230, 360
AlwI GGATC 1 cut(s) 24
AlwNI CAGNNNCTG 1 cut(s) 230
AoxI GGCC 2 cut(s) 3, 51
AspS9I GGNCC 2 cut(s) 4, 52
AsuHPI GGTGA 3 cut(s) 33, 257, 500
AsuII TTCGAA 1 cut(s) 393
AsuNHI GCTAGC 1 cut(s) 356
BaeI ACNNNNGTAYC 2 cut(s) 259, 292
BanII GRGCYC 1 cut(s) 66
BbsI GAAGAC 2 cut(s) 489, 555
BccI CCATC 3 cut(s) 194, 392, 526
BfaI CTAG 3 cut(s) 56, 357, 604
BfmI CTRYAG 2 cut(s) 240, 369
BfuAI ACCTGC 1 cut(s) 445
BglII AGATCT 1 cut(s) 343
BmgT120I GGNCC 2 cut(s) 4, 52
BmtI GCTAGC 1 cut(s) 360
BoxI GACNNNNGTC 1 cut(s) 318
BpiI GAAGAC 2 cut(s) 489, 555
Bpu14I TTCGAA 1 cut(s) 393
BsaBI GATNNNNATC 1 cut(s) 342
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 443, 485
Bse118I RCCGGY 1 cut(s) 436
Bse8I GATNNNNATC 1 cut(s) 342
BseGI GGATG 1 cut(s) 96
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 3 cut(s) 60, 443, 485
BseYI CCCAGC 1 cut(s) 59
BshFI GGCC 2 cut(s) 5, 53
BsiSI CCGG 3 cut(s) 437, 470, 479
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 3 cut(s) 60, 443, 485
BsmFI GGGAC 1 cut(s) 164
BsnI GGCC 2 cut(s) 5, 53
Bsp119I TTCGAA 1 cut(s) 393
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 4 cut(s) 29, 288, 343, 553
BspANI GGCC 2 cut(s) 5, 53
BspMI ACCTGC 1 cut(s) 445
BspOI GCTAGC 1 cut(s) 360
BspPI GGATC 1 cut(s) 24
BspT104I TTCGAA 1 cut(s) 393
BsrFI RCCGGY 1 cut(s) 436
BssAI RCCGGY 1 cut(s) 436
BssMI GATC 4 cut(s) 29, 288, 343, 553
Bst4CI ACNGT 2 cut(s) 388, 488
BstBI TTCGAA 1 cut(s) 393
BstC8I GCNNGC 1 cut(s) 358
BstDEI CTNAG 1 cut(s) 361
BstF5I GGATG 1 cut(s) 96
BstKTI GATC 4 cut(s) 32, 291, 346, 556
BstMBI GATC 4 cut(s) 29, 288, 343, 553
BstNSI RCATGY 2 cut(s) 115, 421
BstPAI GACNNNNGTC 1 cut(s) 318
BstSFI CTRYAG 2 cut(s) 240, 369
BstV2I GAAGAC 2 cut(s) 489, 555
BstX2I RGATCY 2 cut(s) 29, 343
BstXI CCANNNNNNTGG 1 cut(s) 14
BstYI RGATCY 2 cut(s) 29, 343
BsuRI GGCC 2 cut(s) 5, 53
BtsCI GGATG 1 cut(s) 96
BveI ACCTGC 1 cut(s) 445
Cac8I GCNNGC 1 cut(s) 358
CaiI CAGNNNCTG 1 cut(s) 230
Cfr10I RCCGGY 1 cut(s) 436
Cfr13I GGNCC 2 cut(s) 4, 52
Csp6I GTAC 2 cut(s) 191, 306
CviAII CATG 6 cut(s) 112, 124, 175, 280, 418, 524
CviQI GTAC 2 cut(s) 191, 306
DdeI CTNAG 1 cut(s) 361
DpnI GATC 4 cut(s) 31, 290, 345, 555
DpnII GATC 4 cut(s) 29, 288, 343, 553
DrdI GACNNNNNNGTC 1 cut(s) 533
DseDI GACNNNNNNGTC 1 cut(s) 533
Eco24I GRGCYC 1 cut(s) 66
EcoO109I RGGNCCY 1 cut(s) 52
EcoT22I ATGCAT 1 cut(s) 125
EcoT38I GRGCYC 1 cut(s) 66
FaeI CATG 6 cut(s) 115, 127, 178, 283, 421, 527
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FaqI GGGAC 1 cut(s) 164
FatI CATG 6 cut(s) 111, 123, 174, 279, 417, 523
FblI GTMKAC 1 cut(s) 372
FokI GGATG 1 cut(s) 103
FriOI GRGCYC 1 cut(s) 66
FspBI CTAG 3 cut(s) 56, 357, 604
GsaI CCCAGC 1 cut(s) 63
HaeIII GGCC 2 cut(s) 5, 53
HapII CCGG 3 cut(s) 437, 470, 479
Hin1II CATG 6 cut(s) 115, 127, 178, 283, 421, 527
HinfI GANTC 2 cut(s) 204, 338
HpaII CCGG 3 cut(s) 437, 470, 479
HphI GGTGA 3 cut(s) 33, 257, 500
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 3 cut(s) 226, 343, 576
Hpy188III TCNNGA 1 cut(s) 197
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 2 cut(s) 55, 333
HpyCH4III ACNGT 2 cut(s) 388, 488
HpyCH4IV ACGT 1 cut(s) 591
HpyCH4V TGCA 5 cut(s) 20, 78, 123, 174, 407
HpyF3I CTNAG 1 cut(s) 361
HpySE526I ACGT 1 cut(s) 591
Hsp92II CATG 6 cut(s) 115, 127, 178, 283, 421, 527
Kzo9I GATC 4 cut(s) 29, 288, 343, 553
LpnPI CCDG 6 cut(s) 20, 45, 440, 450, 483, 492
MaeI CTAG 3 cut(s) 56, 357, 604
MaeII ACGT 1 cut(s) 591
MaeIII GTNAC 1 cut(s) 39
MalI GATC 4 cut(s) 31, 290, 345, 555
MboI GATC 4 cut(s) 29, 288, 343, 553
MboII GAAGA 3 cut(s) 407, 494, 560
MflI RGATCY 2 cut(s) 29, 343
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 4 cut(s) 169, 462, 491, 510
MmeI TCCRAC 1 cut(s) 57
MnlI CCTC 3 cut(s) 43, 265, 358
Mph1103I ATGCAT 1 cut(s) 125
MslI CAYNNNNRTG 3 cut(s) 128, 198, 416
MspI CCGG 3 cut(s) 437, 470, 479
NdeII GATC 4 cut(s) 29, 288, 343, 553
NheI GCTAGC 1 cut(s) 356
NlaIII CATG 6 cut(s) 115, 127, 178, 283, 421, 527
NmuCI GTSAC 1 cut(s) 39
NsiI ATGCAT 1 cut(s) 125
NspI RCATGY 2 cut(s) 115, 421
NspV TTCGAA 1 cut(s) 393
PciI ACATGT 1 cut(s) 111
PcsI WCGNNNNNNNCGW 2 cut(s) 297, 306
PfeI GAWTC 2 cut(s) 204, 338
PscI ACATGT 1 cut(s) 111
PshAI GACNNNNGTC 1 cut(s) 318
PspFI CCCAGC 1 cut(s) 59
PspPI GGNCC 2 cut(s) 4, 52
PstNI CAGNNNCTG 1 cut(s) 230
PsuI RGATCY 2 cut(s) 29, 343
RsaI GTAC 2 cut(s) 192, 307
RsaNI GTAC 2 cut(s) 191, 306
RseI CAYNNNNRTG 3 cut(s) 128, 198, 416
Sau3AI GATC 4 cut(s) 29, 288, 343, 553
Sau96I GGNCC 2 cut(s) 4, 52
SduI GDGCHC 1 cut(s) 66
SfcI CTRYAG 2 cut(s) 240, 369
SfuI TTCGAA 1 cut(s) 393
SmiMI CAYNNNNRTG 3 cut(s) 128, 198, 416
Sse9I AATT 4 cut(s) 169, 462, 491, 510
SspMI CTAG 3 cut(s) 56, 357, 604
TaaI ACNGT 2 cut(s) 388, 488
TaiI ACGT 1 cut(s) 594
TaqI TCGA 4 cut(s) 48, 291, 300, 393
TasI AATT 4 cut(s) 169, 462, 491, 510
TfiI GAWTC 2 cut(s) 204, 338
TseFI GTSAC 1 cut(s) 39
Tsp45I GTSAC 1 cut(s) 39
TspDTI ATGAA 2 cut(s) 347, 602
XceI RCATGY 2 cut(s) 115, 421
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 3 cut(s) 56, 357, 604
Zsp2I ATGCAT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.