MD15G1111100.v1.1

cwf21 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002497
Physical Location & Seq
Reverse (-)
7749460 .. 7750455
996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1111100.v1.1.491

Sequence Viewer

Length: 732 bp
ATGTACAACGGAATAGGGTTACAGACTCCGAGGGGGTCGGGGACCAATGGCTACATCCAGACCAACAAGTTCTTCATCAAATCGGGGACTGGGAAGGTCGCCGACTCCTCCCGCGGTTTCGACGATGACCAGGGCACCGGCGGCGTCACCAAGAAGCCCAACAGAGACATCCTCGAGCACGATCGGAAGCGCCATATTGAGCTCAAGCTTGTCGTGCTCGAAGATAAGCTCATCGATCAGGGTTTTACCGACGCTGAGATCGCCGAGAAACTTGACGAGGCTCGGAAGACTCTCGAAGCTGCTGCGGCTTCGGAGGAGCGTAGCGGACCTGGCGCCATTGTTGTGTCCGATAAGAAGGTTTCAGATACGCAGACCCACCAAATTGCCGCTAGGAAGGAGAAACAAATGGAAACATTAAGAGCTGTTCTCGGGCTTGCTGCGCCCGAATCAGCTGACAAGAATACTGATGATATCGAAGATGGACTGAAAAGTAGTCGAAAGAATGGTCCAGAAGACGATGGCAAGTTTCAAGAGAGAAGTGAGCATGCATTTTTGGACAGGGACTATGGGCGGAAGAAACATGGGGAGGAAAAGCAGAAAGATGAGAAAGATGATAAGAAGAAGAGAGTTAAAGAATCTAAGCGAAACAAGATGGAAGAAGGTAGTAAGAGAAGGCATAAATATGATTCTTCTGATACCGAAAGCAGTGGCAGTCCTCCTCCCAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cwf21

Protein Analysis

244

Amino Acids

27.07

Weight (kDa)

7.81

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf21 PF08312 58 - 100 7.9e-15 cwf21 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 134, 332
AccII CGCG 1 cut(s) 114
AciI CCGC 7 cut(s) 112, 114, 141, 305, 324, 387, 571
AcyI GRCGYC 2 cut(s) 144, 333
AfaI GTAC 1 cut(s) 5
AgsI TTSAA 1 cut(s) 530
AjnI CCWGG 2 cut(s) 129, 328
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 7 cut(s) 202, 208, 229, 299, 422, 452, 727
AluI AGCT 7 cut(s) 202, 208, 229, 299, 422, 452, 727
Alw21I GWGCWC 3 cut(s) 180, 204, 219
Alw26I GTCTC 1 cut(s) 159
Ama87I CYCGRG 2 cut(s) 173, 428
ApeKI GCWGC 3 cut(s) 299, 302, 437
ArsI GACNNNNNNTTYG 2 cut(s) 490, 522
AspLEI GCGC 3 cut(s) 192, 335, 442
AspS9I GGNCC 3 cut(s) 42, 326, 506
AsuHPI GGTGA 1 cut(s) 139
AvaI CYCGRG 2 cut(s) 173, 428
AvaII GGWCC 3 cut(s) 42, 326, 506
BaeGI GKGCMC 1 cut(s) 137
BanI GGYRCC 2 cut(s) 134, 332
BanII GRGCYC 1 cut(s) 204
BbsI GAAGAC 2 cut(s) 293, 519
Bbv12I GWGCWC 3 cut(s) 180, 204, 219
BbvI GCAGC 3 cut(s) 286, 289, 424
BccI CCATC 3 cut(s) 473, 512, 646
BcgI CGANNNNNNTGC 2 cut(s) 284, 318
BciT130I CCWGG 2 cut(s) 131, 330
BcoDI GTCTC 1 cut(s) 159
BfaI CTAG 1 cut(s) 390
BfoI RGCGCY 2 cut(s) 193, 336
BisI GCNGC 6 cut(s) 142, 300, 303, 306, 387, 438
BlsI GCNGC 6 cut(s) 143, 301, 304, 307, 388, 439
Bme1390I CCNGG 2 cut(s) 131, 330
Bme18I GGWCC 3 cut(s) 42, 326, 506
BmeT110I CYCGRG 2 cut(s) 173, 428
BmgT120I GGNCC 3 cut(s) 42, 326, 506
BmiI GGNNCC 3 cut(s) 43, 136, 334
BmrFI CCNGG 2 cut(s) 131, 330
BmrI ACTGGG 1 cut(s) 99
BmuI ACTGGG 1 cut(s) 99
BpiI GAAGAC 2 cut(s) 293, 519
BplI GAGNNNNNCTC 4 cut(s) 156, 188, 411, 443
BpuEI CTTGAG 1 cut(s) 188
Bsa29I ATCGAT 1 cut(s) 234
BsaHI GRCGYC 2 cut(s) 144, 333
BsaJI CCNNGG 3 cut(s) 29, 112, 130
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 1 cut(s) 94
BseBI CCWGG 2 cut(s) 131, 330
BseCI ATCGAT 1 cut(s) 234
BseDI CCNNGG 3 cut(s) 29, 112, 130
BseGI GGATG 2 cut(s) 54, 168
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 3 cut(s) 97, 329, 708
BseSI GKGCMC 1 cut(s) 137
BseXI GCAGC 3 cut(s) 286, 289, 424
Bsh1236I CGCG 1 cut(s) 114
Bsh1285I CGRYCG 1 cut(s) 184
BshNI GGYRCC 2 cut(s) 134, 332
BshVI ATCGAT 1 cut(s) 234
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 3 cut(s) 180, 204, 219
BsiHKCI CYCGRG 2 cut(s) 173, 428
BsiSI CCGG 1 cut(s) 138
BslFI GGGAC 3 cut(s) 55, 100, 575
BsmAI GTCTC 1 cut(s) 159
BsmFI GGGAC 3 cut(s) 55, 100, 575
BsoBI CYCGRG 2 cut(s) 173, 428
Bsp1286I GDGCHC 4 cut(s) 137, 180, 204, 219
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 181, 235, 258
BspACI CCGC 7 cut(s) 112, 114, 141, 305, 324, 387, 571
BspCNI CTCAG 1 cut(s) 247
BspDI ATCGAT 1 cut(s) 234
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 3 cut(s) 43, 136, 334
BspT107I GGYRCC 2 cut(s) 134, 332
BsrFI RCCGGY 1 cut(s) 137
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 94
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 3 cut(s) 29, 112, 130
BssMI GATC 3 cut(s) 181, 235, 258
BssNI GRCGYC 2 cut(s) 144, 333
Bst2UI CCWGG 2 cut(s) 131, 330
Bst6I CTCTTC 1 cut(s) 617
BstACI GRCGYC 2 cut(s) 144, 333
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 435, 546
BstDEI CTNAG 2 cut(s) 255, 639
BstDSI CCRYGG 1 cut(s) 112
BstF5I GGATG 2 cut(s) 54, 168
BstFNI CGCG 1 cut(s) 114
BstH2I RGCGCY 2 cut(s) 193, 336
BstHHI GCGC 3 cut(s) 192, 335, 442
BstKTI GATC 3 cut(s) 184, 238, 261
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 3 cut(s) 181, 235, 258
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 6 cut(s) 141, 214, 260, 305, 330, 439
BstNI CCWGG 2 cut(s) 131, 330
BstNSI RCATGY 1 cut(s) 548
BstSCI CCNGG 2 cut(s) 129, 328
BstSLI GKGCMC 1 cut(s) 137
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 3 cut(s) 286, 289, 424
BstV2I GAAGAC 2 cut(s) 293, 519
Bsu15I ATCGAT 1 cut(s) 234
BsuTUI ATCGAT 1 cut(s) 234
BtgI CCRYGG 1 cut(s) 112
BtsCI GGATG 2 cut(s) 54, 168
BtsI GCAGTG 1 cut(s) 712
BtsIMutI CAGTG 1 cut(s) 712
Cac8I GCNNGC 2 cut(s) 435, 546
CfoI GCGC 3 cut(s) 192, 335, 442
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 3 cut(s) 42, 326, 506
Cfr42I CCGCGG 1 cut(s) 115
ClaI ATCGAT 1 cut(s) 234
CseI GACGC 2 cut(s) 133, 260
Csp6I GTAC 1 cut(s) 4
CviAII CATG 2 cut(s) 545, 581
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 2 cut(s) 255, 639
DinI GGCGCC 1 cut(s) 334
DpnI GATC 3 cut(s) 183, 237, 260
DpnII GATC 3 cut(s) 181, 235, 258
Eam1104I CTCTTC 1 cut(s) 617
EarI CTCTTC 1 cut(s) 617
EciI GGCGGA 1 cut(s) 586
Ecl136II GAGCTC 1 cut(s) 202
Eco24I GRGCYC 1 cut(s) 204
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 3 cut(s) 42, 326, 506
Eco53kI GAGCTC 1 cut(s) 202
Eco88I CYCGRG 2 cut(s) 173, 428
EcoICRI GAGCTC 1 cut(s) 202
EcoRII CCWGG 2 cut(s) 129, 328
EcoRV GATATC 1 cut(s) 472
EcoT22I ATGCAT 1 cut(s) 550
EcoT38I GRGCYC 1 cut(s) 204
EgeI GGCGCC 1 cut(s) 334
EheI GGCGCC 1 cut(s) 334
FaeI CATG 2 cut(s) 548, 584
FaiI YATR 6 cut(s) 195, 546, 567, 582, 678, 684
FaqI GGGAC 3 cut(s) 55, 100, 575
FatI CATG 2 cut(s) 544, 580
FauI CCCGC 1 cut(s) 119
Fnu4HI GCNGC 6 cut(s) 142, 300, 303, 306, 387, 438
FokI GGATG 2 cut(s) 41, 155
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 6 cut(s) 142, 300, 303, 306, 387, 438
FspBI CTAG 1 cut(s) 390
GlaI GCGC 3 cut(s) 191, 334, 441
GluI GCNGC 6 cut(s) 142, 300, 303, 306, 387, 438
HaeII RGCGCY 2 cut(s) 193, 336
HapII CCGG 1 cut(s) 138
HgaI GACGC 2 cut(s) 133, 260
HhaI GCGC 3 cut(s) 192, 335, 442
Hin1I GRCGYC 2 cut(s) 144, 333
Hin1II CATG 2 cut(s) 548, 584
Hin6I GCGC 3 cut(s) 190, 333, 440
HinP1I GCGC 3 cut(s) 190, 333, 440
HindIII AAGCTT 2 cut(s) 206, 725
HinfI GANTC 6 cut(s) 25, 104, 289, 446, 635, 686
HpaII CCGG 1 cut(s) 138
HphI GGTGA 1 cut(s) 139
Hpy188I TCNGA 7 cut(s) 30, 186, 285, 313, 349, 364, 694
Hpy188III TCNNGA 4 cut(s) 58, 293, 509, 530
Hpy99I CGWCG 2 cut(s) 125, 254
HpyAV CCTTC 5 cut(s) 88, 349, 388, 653, 666
HpyCH4V TGCA 1 cut(s) 548
HpyF10VI GCNNNNNNNGC 6 cut(s) 141, 214, 260, 305, 330, 439
HpyF3I CTNAG 2 cut(s) 255, 639
Hsp92I GRCGYC 2 cut(s) 144, 333
Hsp92II CATG 2 cut(s) 548, 584
HspAI GCGC 3 cut(s) 190, 333, 440
KasI GGCGCC 1 cut(s) 332
KspI CCGCGG 1 cut(s) 115
Kzo9I GATC 3 cut(s) 181, 235, 258
LmnI GCTCC 1 cut(s) 316
Lsp1109I GCAGC 3 cut(s) 286, 289, 424
MaeI CTAG 1 cut(s) 390
MaeIII GTNAC 2 cut(s) 18, 145
MalI GATC 3 cut(s) 183, 237, 260
MboI GATC 3 cut(s) 181, 235, 258
MhlI GDGCHC 4 cut(s) 137, 180, 204, 219
MluCI AATT 1 cut(s) 381
Mly113I GGCGCC 1 cut(s) 333
MlyI GAGTC 3 cut(s) 19, 98, 283
MnlI CCTC 8 cut(s) 24, 118, 182, 271, 307, 580, 726, 729
Mph1103I ATGCAT 1 cut(s) 550
MseI TTAA 2 cut(s) 416, 630
MslI CAYNNNNRTG 2 cut(s) 341, 681
MspA1I CMGCKG 2 cut(s) 114, 452
MspI CCGG 1 cut(s) 138
MspR9I CCNGG 2 cut(s) 131, 330
MvaI CCWGG 2 cut(s) 131, 330
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 6 cut(s) 141, 214, 260, 305, 330, 439
NarI GGCGCC 1 cut(s) 333
NdeII GATC 3 cut(s) 181, 235, 258
NlaIII CATG 2 cut(s) 548, 584
NlaIV GGNNCC 3 cut(s) 43, 136, 334
NmeAIII GCCGAG 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 145
NsiI ATGCAT 1 cut(s) 550
NspI RCATGY 1 cut(s) 548
PaeI GCATGC 1 cut(s) 548
PaeR7I CTCGAG 1 cut(s) 173
PfeI GAWTC 3 cut(s) 446, 635, 686
PkrI GCNGC 6 cut(s) 143, 301, 304, 307, 388, 439
Ple19I CGATCG 1 cut(s) 184
PleI GAGTC 3 cut(s) 19, 98, 283
PluTI GGCGCC 1 cut(s) 336
PpsI GAGTC 3 cut(s) 19, 98, 283
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 2 cut(s) 129, 328
PspGI CCWGG 2 cut(s) 129, 328
PspN4I GGNNCC 3 cut(s) 43, 136, 334
PspPI GGNCC 3 cut(s) 42, 326, 506
PspXI VCTCGAGB 1 cut(s) 173
PvuI CGATCG 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 2 cut(s) 341, 681
SacI GAGCTC 1 cut(s) 204
SacII CCGCGG 1 cut(s) 115
SaqAI TTAA 2 cut(s) 416, 630
SatI GCNGC 6 cut(s) 142, 300, 303, 306, 387, 438
Sau3AI GATC 3 cut(s) 181, 235, 258
Sau96I GGNCC 3 cut(s) 42, 326, 506
SchI GAGTC 3 cut(s) 19, 98, 283
ScrFI CCNGG 2 cut(s) 131, 330
SduI GDGCHC 4 cut(s) 137, 180, 204, 219
SfoI GGCGCC 1 cut(s) 334
Sfr274I CTCGAG 1 cut(s) 173
Sfr303I CCGCGG 1 cut(s) 115
SgrAI CRCCGGYG 1 cut(s) 137
SgrBI CCGCGG 1 cut(s) 115
SinI GGWCC 3 cut(s) 42, 326, 506
SlaI CTCGAG 1 cut(s) 173
SmiMI CAYNNNNRTG 2 cut(s) 341, 681
SmlI CTYRAG 2 cut(s) 173, 203
SmoI CTYRAG 2 cut(s) 173, 203
SphI GCATGC 1 cut(s) 548
Sse9I AATT 1 cut(s) 381
SsiI CCGC 7 cut(s) 112, 114, 141, 305, 324, 387, 571
SspDI GGCGCC 1 cut(s) 332
SspMI CTAG 1 cut(s) 390
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 2 cut(s) 129, 328
TaqI TCGA 7 cut(s) 120, 174, 219, 234, 294, 474, 496
TasI AATT 1 cut(s) 381
TatI WGTACW 1 cut(s) 3
TauI GCSGC 3 cut(s) 144, 308, 389
TfiI GAWTC 3 cut(s) 446, 635, 686
Tru1I TTAA 2 cut(s) 416, 630
Tru9I TTAA 2 cut(s) 416, 630
TscAI CASTG 1 cut(s) 712
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 3 cut(s) 299, 302, 437
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 1 cut(s) 64
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 1 cut(s) 712
VpaK11BI GGWCC 3 cut(s) 42, 326, 506
XceI RCATGY 1 cut(s) 548
XhoI CTCGAG 1 cut(s) 173
XspI CTAG 1 cut(s) 390
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.