RchiOBHm_Chr4g0410401

cwf21 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002497
Physical Location & Seq
Forward (+)
34151947 .. 34153171
1225 bp
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UTR
Exon/CDS
Intron
PRQ38132

Sequence Viewer

Length: 324 bp
ATGTACAACGGAATAGGGTTACAGACTCCGAGAGGGTCTGGAACCAATGGCAATATCCAGACGAACAAGTTCTTCATCAGGTCCAAGACTGGCAAGGTTGCTGAAAACTCCAGAGGGTTTGAGGGAGACCAGGGCATGGCTGGCGTCACAAAGAAGGCCAACAGAGACATCCTTGAGCATGATCGCAAGCGCCAGATTGAGCTCAAGCTTGTGGTGCTGGAGGAGAATCTGAGCGATCAGGGATTCACGGAGGCTGAGATTGGTGAGAGAAGGTGGCCGAGGCTCGGAGGACTCTGGAAGCTGCTGCCGCGTCTGAGGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cwf21

Protein Analysis

107

Amino Acids

12.12

Weight (kDa)

10.15

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf21 PF08312 58 - 90 3.7e-11 cwf21 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 136
AccII CGCG 1 cut(s) 310
AciI CCGC 1 cut(s) 308
AcoI YGGCCR 1 cut(s) 275
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 136, 284
AjnI CCWGG 1 cut(s) 129
AluBI AGCT 3 cut(s) 202, 208, 301
AluI AGCT 3 cut(s) 202, 208, 301
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 120, 159
AoxI GGCC 2 cut(s) 156, 275
ApeKI GCWGC 2 cut(s) 301, 304
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 1 cut(s) 81
AsuHPI GGTGA 1 cut(s) 275
AvaII GGWCC 1 cut(s) 81
BanII GRGCYC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 288, 291
BciT130I CCWGG 1 cut(s) 131
BcoDI GTCTC 2 cut(s) 120, 159
BfoI RGCGCY 1 cut(s) 193
BisI GCNGC 3 cut(s) 302, 305, 308
BlsI GCNGC 3 cut(s) 303, 306, 309
Bme1390I CCNGG 1 cut(s) 131
Bme18I GGWCC 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 81
BmiI GGNNCC 1 cut(s) 43
BmrFI CCNGG 1 cut(s) 131
BpmI CTGGAG 2 cut(s) 94, 239
BpuEI CTTGAG 2 cut(s) 188, 194
BsaHI GRCGYC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 130, 278
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 284
Bse1I ACTGG 1 cut(s) 94
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 2 cut(s) 130, 278
BseGI GGATG 1 cut(s) 168
BseLI CCNNNNNNNGG 2 cut(s) 136, 284
BseMII CTCAG 3 cut(s) 221, 246, 305
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 2 cut(s) 288, 291
Bsh1236I CGCG 1 cut(s) 310
BshFI GGCC 2 cut(s) 158, 277
BsiHKAI GWGCWC 1 cut(s) 204
BslI CCNNNNNNNGG 2 cut(s) 136, 284
BsmAI GTCTC 2 cut(s) 120, 159
BsnI GGCC 2 cut(s) 158, 277
Bso31I GGTCTC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 204
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 181, 235
BspACI CCGC 1 cut(s) 308
BspANI GGCC 2 cut(s) 158, 277
BspCNI CTCAG 3 cut(s) 222, 247, 306
BspFNI CGCG 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 43
BspTNI GGTCTC 1 cut(s) 120
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 94
BssECI CCNNGG 2 cut(s) 130, 278
BssMI GATC 2 cut(s) 181, 235
BssNI GRCGYC 1 cut(s) 144
Bst2UI CCWGG 1 cut(s) 131
BstACI GRCGYC 1 cut(s) 144
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 142, 188
BstDEI CTNAG 3 cut(s) 230, 255, 314
BstF5I GGATG 1 cut(s) 168
BstFNI CGCG 1 cut(s) 310
BstH2I RGCGCY 1 cut(s) 193
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 2 cut(s) 184, 238
BstMAI GTCTC 2 cut(s) 120, 159
BstMBI GATC 2 cut(s) 181, 235
BstMWI GCNNNNNNNGC 3 cut(s) 141, 214, 307
BstNI CCWGG 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 129
BstUI CGCG 1 cut(s) 310
BstV1I GCAGC 2 cut(s) 288, 291
BsuRI GGCC 2 cut(s) 158, 277
BtsCI GGATG 1 cut(s) 168
Cac8I GCNNGC 2 cut(s) 142, 188
CfoI GCGC 1 cut(s) 192
Cfr13I GGNCC 1 cut(s) 81
CseI GACGC 2 cut(s) 133, 299
Csp6I GTAC 1 cut(s) 4
CviAII CATG 2 cut(s) 136, 179
CviJI RGCY 8 cut(s) 140, 158, 202, 208, 254, 277, 283, 301
CviKI_1 RGCY 8 cut(s) 140, 158, 202, 208, 254, 277, 283, 301
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 3 cut(s) 230, 255, 314
DpnI GATC 2 cut(s) 183, 237
DpnII GATC 2 cut(s) 181, 235
EaeI YGGCCR 1 cut(s) 275
Ecl136II GAGCTC 1 cut(s) 202
Eco24I GRGCYC 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 81
Eco53kI GAGCTC 1 cut(s) 202
EcoICRI GAGCTC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 129
EcoT38I GRGCYC 1 cut(s) 204
FaeI CATG 2 cut(s) 139, 182
FaiI YATR 2 cut(s) 137, 180
FatI CATG 2 cut(s) 135, 178
Fnu4HI GCNGC 3 cut(s) 302, 305, 308
FokI GGATG 1 cut(s) 155
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 3 cut(s) 302, 305, 308
GlaI GCGC 1 cut(s) 191
GluI GCNGC 3 cut(s) 302, 305, 308
GsuI CTGGAG 2 cut(s) 94, 239
HaeII RGCGCY 1 cut(s) 193
HaeIII GGCC 2 cut(s) 158, 277
HgaI GACGC 2 cut(s) 133, 299
HhaI GCGC 1 cut(s) 192
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 2 cut(s) 139, 182
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 4 cut(s) 25, 226, 243, 291
HphI GGTGA 1 cut(s) 275
Hpy188I TCNGA 4 cut(s) 30, 231, 287, 315
Hpy188III TCNNGA 4 cut(s) 39, 58, 111, 295
HpyAV CCTTC 2 cut(s) 148, 264
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 214, 307
HpyF3I CTNAG 3 cut(s) 230, 255, 314
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 2 cut(s) 139, 182
HspAI GCGC 1 cut(s) 190
Kzo9I GATC 2 cut(s) 181, 235
Lsp1109I GCAGC 2 cut(s) 288, 291
MaeIII GTNAC 2 cut(s) 18, 145
MalI GATC 2 cut(s) 183, 237
MboI GATC 2 cut(s) 181, 235
MboII GAAGA 1 cut(s) 64
MhlI GDGCHC 1 cut(s) 204
MlyI GAGTC 2 cut(s) 19, 285
MnlI CCTC 8 cut(s) 26, 107, 115, 214, 244, 273, 281, 309
MroXI GAANNNNTTC 1 cut(s) 68
MspR9I CCNGG 1 cut(s) 131
MvaI CCWGG 1 cut(s) 131
MvnI CGCG 1 cut(s) 310
MwoI GCNNNNNNNGC 3 cut(s) 141, 214, 307
NdeII GATC 2 cut(s) 181, 235
NlaIII CATG 2 cut(s) 139, 182
NlaIV GGNNCC 1 cut(s) 43
NmeAIII GCCGAG 1 cut(s) 303
NmuCI GTSAC 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 68
PfeI GAWTC 2 cut(s) 226, 243
PflMI CCANNNNNTGG 1 cut(s) 136
PkrI GCNGC 3 cut(s) 303, 306, 309
PleI GAGTC 2 cut(s) 19, 285
PpsI GAGTC 2 cut(s) 19, 285
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 1 cut(s) 43
PspPI GGNCC 1 cut(s) 81
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SacI GAGCTC 1 cut(s) 204
SatI GCNGC 3 cut(s) 302, 305, 308
Sau3AI GATC 2 cut(s) 181, 235
Sau96I GGNCC 1 cut(s) 81
SchI GAGTC 2 cut(s) 19, 285
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 1 cut(s) 204
SetI ASST 6 cut(s) 83, 99, 204, 210, 275, 303
SinI GGWCC 1 cut(s) 81
SmlI CTYRAG 2 cut(s) 173, 203
SmoI CTYRAG 2 cut(s) 173, 203
SsiI CCGC 1 cut(s) 308
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 129
TatI WGTACW 1 cut(s) 3
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 2 cut(s) 226, 243
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 2 cut(s) 301, 304
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 1 cut(s) 64
TspGWI ACGGA 2 cut(s) 24, 263
Van91I CCANNNNNTGG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 81
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XmnI GAANNNNTTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.