FvH4_2g30620

cwf21 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002497
Physical Location & Seq
Forward (+)
23673278 .. 23676733
3456 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30620.t3

Sequence Viewer

Length: 2385 bp
ATGTACAACGGCATAGGGTTACAGACTCCGAGAGGGTCAGGAACCAATGGCTACATCCAGACGAACAAGTTCTTCATCAAATCCAAGACCGGCAAGGCCGACGGTAACTCCCGAGGCTTTGAAGGCGACCAAGGCATGGCCGGCGTCTCCAAGAAGGCGAACCGTGACATCCTCGAGCATGACCGCAAGCGCCAGATTGAGCTCAAGCTCGTCGTGCTGGAAGACAAGCTGACCGATCAGGGATTCACCGACGATGAGATCGCCGAGAAGGTCGCCGAAGTTAGGAGGACTCTCGAAGCCGCCGCCGCCTCGGAGGAGATGGGCGGGCCCACTGCCATTGTTCTCGGAGGAGATAAGAAGGTTTCGGATACACAGACCCACCAGATTGCAGCTAGGAAGGAGAAGCAGATGGAGAACCTAAGAGATGCTCTTAAGCTAAAGCCTGAAGTTACAGAAACAAATGCTGAGGAGATTGAGGAGGGAATGATAATTAGTCCGAGGAATGATGGTAGTAGGTATTATGAGAGGAAAGAGCATTCGTTTTTGGATAGGGATAATGGGTGGAAGAAGTCTGTGGAGGAGAAGCAGAAGGTTGAGAAAGATAAGAAAAAGAGTCTGAAGGAGTCTAGGCGGACGAAAAAGAAAGGAAGCAGGAAGAGAAGGCATAAGGATGATTCTTCTGATACAGATAGCAGTGGTAGCCCTCTGAAACCTGATAAGAAGAAAAAGCGTTGTAGTAGAAGTAGCAGCAGTGAAGAAGATGATTCTGAAGTTGATACATCGAAGAAGGTTGAGAAATACAATAAGAAGAGGTATGATTCAGAAGAAGATGATTCAGATGTTGATGTTGATAAGAAGCGGAAGGTTGTAAAGAAGCATAGTAGAAGCCGAGACGATAGAGCTGATGATTCTGATACCAGTGAGGATGCGGGGGATAGATCCAGAAAGGAAATTAAGAAATCCAAACAACCTCGTAGGAGGCATGATTCAGATGAAGATGATTCTGGAGATGATGTTGACAAGAAGAAAAAGGTTGTAAAGAAGCATAGTAAAAGGCGAGACGATAGAGCTGATGATTCTGATACCAGTGAGGATGCGGGGGCTAGATCCAGAAAGGATATTAAGAAATCCAAACAACCTCGTAGGAGGCATGAATCAGATGAAGATGATTCTGGAGATGATGTTGACAAGAAGAAAAAGGCTGTAAAGAAGCATAGTAAAAGCAGAAACGATGATACTGATGATTCCAGTGCCAGTGAAGATGCCAAAGATAGATCCATAAGGGAAGTCAAGAAACCCGAACAACCTCGTAGGAGGCATGATGCAGATGTAGATGGTTCTGAAGATGATGGTGACAAGAAAAGGAAGGTTGTAAAGAAGCATAGTAAAAGCAGAAATGATGATACTGATGATTCTGCTGCCAGTGAAGATGCCGAGGATAGATCCGCAAAGGAAGTCAAGAAATCACAACAACCTCGCAGGAGGCATGATTCAGATGATGATTCTGGTTATCCTGAAGAATTGCCGAAGAGCAGAATTGAAAAGGGCAAACAACATGTGAAACCAAACAAAAGGCATGACTCTGGGCATGAGGCTGATCTTGACAGCAGAATGGAAAGGAAAAGTAGCCAAATTGAGCTGCAAAGAAATCATCAGGGCATCCGCAGGGTAGATAAAGATGATTCTGACTATGAAAGATCCAGAAAGAGTAGAGGTGCAATAGCAGAAAAGAGTAGAAGAAGTGGCAGAAATGATACTAATGATGATGATACTAATATTGGGAGGAAACCTGGAAGGGTTGAAGATGTTGATAGTGAATCTGACTATGAAAGATCCAGAAAAAGTAGAGGTGCAATTACAGAAAAAAGTAGAAGAAGTGACAGGAATGATGCTAATGATGATACTAATATTGAGAGGAAACCGGAAAGGGTTGAAGAGGGTGATGGAGGACGTGGAAGGCACTACAATGGTGAACTTCAAAGAGGAAGGAAGCATGAAAGAGATGAAGTGGACCATGATTATAGAAGGCCCAGGGAAGGTCTGGAACAGGGTCTTGAAAGTAGAAAGCGAGGTAATAGAGAGGAAAAGGGGGCAAATGAGGATATTGAGAGGGATAGACATATGGATTACAAGAGAGCAAAATATGATGAGTCTAGATCAAGTGAGAGGAGAAGGCATGAAACTGTCAGACATAATGAAGATGGGCAGAAGTCTAGAAGGCATGGAGAAGATGAAGGGGAGCGTAGATACAGAAGACATGAAAACCTGGAGGAACAACGGGAAAATAAGGAGACTGAGAGAGATAGACAGGATTCCAGATCAAGTGAGAGAAGAAGGTACGGGAATGACAAGCACAATGACAGTCGGTCTAGGCATCGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cwf21

Protein Analysis

795

Amino Acids

92.23

Weight (kDa)

9.11

Isoelectric Point (pI)

69.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf21 PF08312 58 - 100 8.9e-16 cwf21 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 136
AclWI GGATC 6 cut(s) 933, 1101, 1269, 1437, 1692, 1827
AcoI YGGCCR 1 cut(s) 138
AcuI CTGAAG 5 cut(s) 465, 638, 789, 1362, 1536
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 2 cut(s) 5, 2339
AfiI CCNNNNNNNGG 3 cut(s) 136, 282, 2036
AflII CTTAAG 1 cut(s) 431
AflIII ACRYGT 1 cut(s) 1555
AgsI TTSAA 6 cut(s) 122, 1541, 1802, 1934, 1979, 2057
AhdI GACNNNNNGTC 1 cut(s) 2365
AjiI CACGTC 1 cut(s) 1952
AjnI CCWGG 3 cut(s) 1789, 2030, 2265
AluBI AGCT 8 cut(s) 202, 208, 229, 392, 436, 902, 1070, 1639
AluI AGCT 8 cut(s) 202, 208, 229, 392, 436, 902, 1070, 1639
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 4 cut(s) 151, 885, 1053, 2285
AlwI GGATC 6 cut(s) 933, 1101, 1269, 1437, 1692, 1827
Ama87I CYCGRG 2 cut(s) 111, 173
AoxI GGCC 4 cut(s) 96, 138, 326, 2027
ApaI GGGCCC 1 cut(s) 330
ApeKI GCWGC 4 cut(s) 389, 747, 1418, 1639
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 4 cut(s) 326, 327, 2011, 2028
AsuHPI GGTGA 4 cut(s) 238, 1364, 1952, 1982
AvaI CYCGRG 2 cut(s) 111, 173
AvaII GGWCC 1 cut(s) 2011
BaeGI GKGCMC 1 cut(s) 330
BanII GRGCYC 2 cut(s) 204, 330
BbsI GAAGAC 2 cut(s) 228, 2260
Bbv12I GWGCWC 1 cut(s) 204
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 4 cut(s) 401, 759, 1405, 1626
BccI CCATC 7 cut(s) 313, 403, 500, 1328, 1343, 1937, 2195
BceAI ACGGC 1 cut(s) 25
BciT130I CCWGG 3 cut(s) 1791, 2032, 2267
BciVI GTATCC 1 cut(s) 361
BcoDI GTCTC 4 cut(s) 151, 885, 1053, 2285
BfaI CTAG 6 cut(s) 393, 627, 1104, 2154, 2214, 2370
BfoI RGCGCY 1 cut(s) 193
BfrI CTTAAG 1 cut(s) 431
BfuI GTATCC 1 cut(s) 361
BisI GCNGC 7 cut(s) 300, 303, 306, 390, 748, 1419, 1640
BlsI GCNGC 7 cut(s) 301, 304, 307, 391, 749, 1420, 1641
Bme1390I CCNGG 3 cut(s) 1791, 2032, 2267
Bme18I GGWCC 1 cut(s) 2011
BmeRI GACNNNNNGTC 1 cut(s) 2365
BmeT110I CYCGRG 2 cut(s) 111, 173
BmgBI CACGTC 1 cut(s) 1952
BmgT120I GGNCC 4 cut(s) 326, 327, 2011, 2028
BmiI GGNNCC 2 cut(s) 43, 328
BmrFI CCNGG 3 cut(s) 1791, 2032, 2267
BmsI GCATC 8 cut(s) 415, 916, 1084, 1252, 1312, 1420, 1668, 1879
BpiI GAAGAC 2 cut(s) 228, 2260
BpmI CTGGAG 3 cut(s) 1026, 1194, 2288
Bpu10I CCTNAGC 1 cut(s) 465
BpuEI CTTGAG 1 cut(s) 188
BsaHI GRCGYC 1 cut(s) 144
BsaJI CCNNGG 7 cut(s) 112, 130, 309, 497, 1434, 2030, 2031
BsaWI WCCGGW 1 cut(s) 1921
BsaXI ACNNNNNCTCC 6 cut(s) 92, 122, 999, 1029, 1167, 1197
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 282, 2036
Bse118I RCCGGY 2 cut(s) 89, 140
Bse1I ACTGG 5 cut(s) 918, 1086, 1248, 1254, 1422
BseBI CCWGG 3 cut(s) 1791, 2032, 2267
BseDI CCNNGG 7 cut(s) 112, 130, 309, 497, 1434, 2030, 2031
BseGI GGATG 6 cut(s) 54, 168, 676, 931, 1099, 1659
BseLI CCNNNNNNNGG 3 cut(s) 136, 282, 2036
BseMII CTCAG 2 cut(s) 456, 2286
BseNI ACTGG 5 cut(s) 918, 1086, 1248, 1254, 1422
BseRI GAGGAG 6 cut(s) 329, 363, 482, 491, 593, 2182
BseSI GKGCMC 1 cut(s) 330
BseXI GCAGC 4 cut(s) 401, 759, 1405, 1626
BshFI GGCC 4 cut(s) 98, 140, 328, 2029
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 2 cut(s) 111, 173
BsiSI CCGG 3 cut(s) 90, 141, 1922
BslI CCNNNNNNNGG 3 cut(s) 136, 282, 2036
BsmAI GTCTC 4 cut(s) 151, 885, 1053, 2285
BsmBI CGTCTC 3 cut(s) 151, 885, 1053
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 4 cut(s) 98, 140, 328, 2029
BsoBI CYCGRG 2 cut(s) 111, 173
Bsp120I GGGCCC 1 cut(s) 326
Bsp1286I GDGCHC 2 cut(s) 204, 330
Bsp1407I TGTACA 1 cut(s) 3
BspANI GGCC 4 cut(s) 98, 140, 328, 2029
BspCNI CTCAG 2 cut(s) 457, 2287
BspLI GGNNCC 2 cut(s) 43, 328
BspPI GGATC 6 cut(s) 933, 1101, 1269, 1437, 1692, 1827
BspQI GCTCTTC 1 cut(s) 1523
BspTI CTTAAG 1 cut(s) 431
BsrFI RCCGGY 2 cut(s) 89, 140
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 5 cut(s) 918, 1086, 1248, 1254, 1422
BssAI RCCGGY 2 cut(s) 89, 140
BssECI CCNNGG 7 cut(s) 112, 130, 309, 497, 1434, 2030, 2031
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 1 cut(s) 130
Bst2UI CCWGG 3 cut(s) 1791, 2032, 2267
Bst4CI ACNGT 4 cut(s) 104, 164, 2185, 2363
Bst6I CTCTTC 4 cut(s) 650, 803, 1523, 1929
BstACI GRCGYC 1 cut(s) 144
BstAFI CTTAAG 1 cut(s) 431
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 3 cut(s) 142, 188, 326
BstDEI CTNAG 3 cut(s) 419, 465, 2295
BstF5I GGATG 6 cut(s) 54, 168, 676, 931, 1099, 1659
BstH2I RGCGCY 1 cut(s) 193
BstHHI GCGC 1 cut(s) 192
BstMAI GTCTC 4 cut(s) 151, 885, 1053, 2285
BstMWI GCNNNNNNNGC 6 cut(s) 123, 132, 141, 214, 305, 699
BstNI CCWGG 3 cut(s) 1791, 2032, 2267
BstNSI RCATGY 1 cut(s) 1559
BstSCI CCNGG 3 cut(s) 1789, 2030, 2265
BstSLI GKGCMC 1 cut(s) 330
BstV1I GCAGC 4 cut(s) 401, 759, 1405, 1626
BstV2I GAAGAC 2 cut(s) 228, 2260
BstX2I RGATCY 6 cut(s) 938, 1106, 1274, 1442, 1697, 1832
BstYI RGATCY 6 cut(s) 938, 1106, 1274, 1442, 1697, 1832
BsuI GTATCC 1 cut(s) 361
BsuRI GGCC 4 cut(s) 98, 140, 328, 2029
BtrI CACGTC 1 cut(s) 1952
BtsCI GGATG 6 cut(s) 54, 168, 676, 931, 1099, 1659
BtsI GCAGTG 3 cut(s) 330, 700, 757
BtsIMutI CAGTG 8 cut(s) 330, 700, 757, 925, 1093, 1255, 1261, 1429
Cac8I GCNNGC 3 cut(s) 142, 188, 326
CfoI GCGC 1 cut(s) 192
Cfr10I RCCGGY 2 cut(s) 89, 140
Cfr13I GGNCC 4 cut(s) 326, 327, 2011, 2028
CseI GACGC 1 cut(s) 133
Csp6I GTAC 2 cut(s) 4, 2338
CspCI CAANNNNNGTGG 2 cut(s) 319, 354
CviQI GTAC 2 cut(s) 4, 2338
DdeI CTNAG 3 cut(s) 419, 465, 2295
DriI GACNNNNNGTC 1 cut(s) 2365
EaeI YGGCCR 1 cut(s) 138
Eam1104I CTCTTC 4 cut(s) 650, 803, 1523, 1929
Eam1105I GACNNNNNGTC 1 cut(s) 2365
EarI CTCTTC 4 cut(s) 650, 803, 1523, 1929
EciI GGCGGA 1 cut(s) 646
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 130
Eco24I GRGCYC 2 cut(s) 204, 330
Eco47I GGWCC 1 cut(s) 2011
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 5 cut(s) 465, 638, 789, 1362, 1536
Eco88I CYCGRG 2 cut(s) 111, 173
EcoICRI GAGCTC 1 cut(s) 202
EcoRII CCWGG 3 cut(s) 1789, 2030, 2265
EcoT14I CCWWGG 1 cut(s) 130
EcoT38I GRGCYC 2 cut(s) 204, 330
ErhI CCWWGG 1 cut(s) 130
Esp3I CGTCTC 3 cut(s) 151, 885, 1053
FauI CCCGC 3 cut(s) 317, 922, 1090
FauNDI CATATG 1 cut(s) 2121
Fnu4HI GCNGC 7 cut(s) 300, 303, 306, 390, 748, 1419, 1640
FokI GGATG 6 cut(s) 41, 155, 683, 938, 1106, 1646
FriOI GRGCYC 2 cut(s) 204, 330
Fsp4HI GCNGC 7 cut(s) 300, 303, 306, 390, 748, 1419, 1640
FspBI CTAG 6 cut(s) 393, 627, 1104, 2154, 2214, 2370
GlaI GCGC 1 cut(s) 191
GluI GCNGC 7 cut(s) 300, 303, 306, 390, 748, 1419, 1640
GsuI CTGGAG 3 cut(s) 1026, 1194, 2288
HaeII RGCGCY 1 cut(s) 193
HaeIII GGCC 4 cut(s) 98, 140, 328, 2029
HapII CCGG 3 cut(s) 90, 141, 1922
HgaI GACGC 1 cut(s) 133
HhaI GCGC 1 cut(s) 192
Hin1I GRCGYC 1 cut(s) 144
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HincII GTYRAC 2 cut(s) 1018, 1186
HindII GTYRAC 2 cut(s) 1018, 1186
HpaII CCGG 3 cut(s) 90, 141, 1922
HphI GGTGA 4 cut(s) 238, 1364, 1952, 1982
Hpy166II GTNNAC 4 cut(s) 1018, 1186, 1973, 2011
Hpy8I GTNNAC 4 cut(s) 1018, 1186, 1973, 2011
Hpy99I CGWCG 3 cut(s) 104, 215, 254
HpyCH4III ACNGT 4 cut(s) 104, 164, 2185, 2363
HpyCH4IV ACGT 1 cut(s) 1951
HpyCH4V TGCA 5 cut(s) 389, 1325, 1642, 1718, 1853
HpyF10VI GCNNNNNNNGC 6 cut(s) 123, 132, 141, 214, 305, 699
HpyF3I CTNAG 3 cut(s) 419, 465, 2295
HpySE526I ACGT 1 cut(s) 1951
Hsp92I GRCGYC 1 cut(s) 144
HspAI GCGC 1 cut(s) 190
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
LguI GCTCTTC 1 cut(s) 1523
LmnI GCTCC 1 cut(s) 2239
Lsp1109I GCAGC 4 cut(s) 401, 759, 1405, 1626
LweI GCATC 8 cut(s) 415, 916, 1084, 1252, 1312, 1420, 1668, 1879
MaeI CTAG 6 cut(s) 393, 627, 1104, 2154, 2214, 2370
MaeII ACGT 1 cut(s) 1951
MaeIII GTNAC 7 cut(s) 18, 104, 164, 448, 1352, 1877, 2378
MflI RGATCY 6 cut(s) 938, 1106, 1274, 1442, 1697, 1832
MhlI GDGCHC 2 cut(s) 204, 330
MluCI AATT 6 cut(s) 489, 951, 1520, 1536, 1632, 1854
MlyI GAGTC 6 cut(s) 19, 283, 622, 632, 1574, 2159
MroNI GCCGGC 1 cut(s) 140
MroXI GAANNNNTTC 1 cut(s) 68
MseI TTAA 3 cut(s) 432, 954, 1122
MslI CAYNNNNRTG 2 cut(s) 669, 1965
MspCI CTTAAG 1 cut(s) 431
MspI CCGG 3 cut(s) 90, 141, 1922
MspR9I CCNGG 3 cut(s) 1791, 2032, 2267
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 3 cut(s) 1791, 2032, 2267
MwoI GCNNNNNNNGC 6 cut(s) 123, 132, 141, 214, 305, 699
NaeI GCCGGC 1 cut(s) 142
NdeI CATATG 1 cut(s) 2121
NgoMIV GCCGGC 1 cut(s) 140
NlaIV GGNNCC 2 cut(s) 43, 328
NmeAIII GCCGAG 3 cut(s) 289, 914, 1459
NmuCI GTSAC 4 cut(s) 164, 1352, 1877, 2378
NspI RCATGY 1 cut(s) 1559
PaeR7I CTCGAG 1 cut(s) 173
PasI CCCWGGG 1 cut(s) 2031
PciI ACATGT 1 cut(s) 1555
PciSI GCTCTTC 1 cut(s) 1523
PctI GAATGC 1 cut(s) 535
PdiI GCCGGC 1 cut(s) 142
PdmI GAANNNNTTC 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 136
PkrI GCNGC 7 cut(s) 301, 304, 307, 391, 749, 1420, 1641
PleI GAGTC 6 cut(s) 19, 283, 621, 631, 1574, 2158
PpsI GAGTC 6 cut(s) 19, 283, 621, 631, 1574, 2158
PscI ACATGT 1 cut(s) 1555
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 3 cut(s) 1789, 2030, 2265
PspGI CCWGG 3 cut(s) 1789, 2030, 2265
PspN4I GGNNCC 2 cut(s) 43, 328
PspOMI GGGCCC 1 cut(s) 326
PspPI GGNCC 4 cut(s) 326, 327, 2011, 2028
PspXI VCTCGAGB 1 cut(s) 173
PsuI RGATCY 6 cut(s) 938, 1106, 1274, 1442, 1697, 1832
RsaI GTAC 2 cut(s) 5, 2339
RsaNI GTAC 2 cut(s) 4, 2338
RseI CAYNNNNRTG 2 cut(s) 669, 1965
SacI GAGCTC 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 1523
SaqAI TTAA 3 cut(s) 432, 954, 1122
SatI GCNGC 7 cut(s) 300, 303, 306, 390, 748, 1419, 1640
Sau96I GGNCC 4 cut(s) 326, 327, 2011, 2028
SchI GAGTC 6 cut(s) 19, 283, 622, 632, 1574, 2159
ScrFI CCNGG 3 cut(s) 1791, 2032, 2267
SduI GDGCHC 2 cut(s) 204, 330
SfaNI GCATC 8 cut(s) 415, 916, 1084, 1252, 1312, 1420, 1668, 1879
Sfr274I CTCGAG 1 cut(s) 173
SinI GGWCC 1 cut(s) 2011
SlaI CTCGAG 1 cut(s) 173
SmiMI CAYNNNNRTG 2 cut(s) 669, 1965
SmlI CTYRAG 3 cut(s) 173, 203, 431
SmoI CTYRAG 3 cut(s) 173, 203, 431
Sse9I AATT 6 cut(s) 489, 951, 1520, 1536, 1632, 1854
SspI AATATT 2 cut(s) 1777, 1909
SspMI CTAG 6 cut(s) 393, 627, 1104, 2154, 2214, 2370
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 3 cut(s) 1789, 2030, 2265
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 4 cut(s) 104, 164, 2185, 2363
TaiI ACGT 1 cut(s) 1954
TaqI TCGA 3 cut(s) 174, 294, 782
TaqII GACCGA 2 cut(s) 248, 2355
TasI AATT 6 cut(s) 489, 951, 1520, 1536, 1632, 1854
TatI WGTACW 1 cut(s) 3
TauI GCSGC 3 cut(s) 302, 305, 308
Tru1I TTAA 3 cut(s) 432, 954, 1122
Tru9I TTAA 3 cut(s) 432, 954, 1122
TscAI CASTG 8 cut(s) 337, 700, 757, 925, 1093, 1255, 1261, 1429
TseFI GTSAC 4 cut(s) 164, 1352, 1877, 2378
TseI GCWGC 4 cut(s) 389, 747, 1418, 1639
Tsp45I GTSAC 4 cut(s) 164, 1352, 1877, 2378
TspRI CASTG 8 cut(s) 337, 700, 757, 925, 1093, 1255, 1261, 1429
Van91I CCANNNNNTGG 1 cut(s) 136
Vha464I CTTAAG 1 cut(s) 431
VpaK11BI GGWCC 1 cut(s) 2011
XbaI TCTAGA 2 cut(s) 2153, 2213
XceI RCATGY 1 cut(s) 1559
XcmI CCANNNNNNNNNTGG 1 cut(s) 2038
XhoI CTCGAG 1 cut(s) 173
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 6 cut(s) 393, 627, 1104, 2154, 2214, 2370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.